BLASTX nr result
ID: Ziziphus21_contig00025098
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Ziziphus21_contig00025098 (407 letters) Database: ./nr 77,306,371 sequences; 28,104,191,420 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_007038731.1| ATP binding protein, putative [Theobroma cac... 125 2e-26 ref|XP_002272050.1| PREDICTED: uncharacterized protein At3g49055... 120 3e-25 ref|XP_002513685.1| ATP binding protein, putative [Ricinus commu... 118 2e-24 ref|XP_011048737.1| PREDICTED: uncharacterized protein At3g49055... 115 1e-23 ref|XP_006421957.1| hypothetical protein CICLE_v10006475mg, part... 115 1e-23 ref|XP_012440140.1| PREDICTED: uncharacterized protein At3g49055... 114 2e-23 gb|KHG09822.1| hypothetical protein F383_13172 [Gossypium arboreum] 114 2e-23 ref|XP_008343766.1| PREDICTED: uncharacterized protein At3g49055... 114 4e-23 ref|XP_008382404.1| PREDICTED: uncharacterized protein At3g49055... 114 4e-23 ref|XP_002322480.2| hypothetical protein POPTR_0015s12920g [Popu... 114 4e-23 ref|XP_006490478.1| PREDICTED: uncharacterized protein At3g49055... 113 5e-23 ref|XP_004309199.1| PREDICTED: uncharacterized protein At3g49055... 113 6e-23 ref|XP_009340052.1| PREDICTED: uncharacterized protein At3g49055... 112 8e-23 ref|XP_008376601.1| PREDICTED: uncharacterized protein At3g49055... 112 1e-22 ref|XP_010060758.1| PREDICTED: uncharacterized protein At3g49055... 112 1e-22 gb|KCW90677.1| hypothetical protein EUGRSUZ_A02769 [Eucalyptus g... 112 1e-22 ref|XP_008234212.1| PREDICTED: uncharacterized protein At3g49055... 111 2e-22 ref|XP_009342947.1| PREDICTED: uncharacterized protein At3g49055... 110 3e-22 ref|XP_010108356.1| hypothetical protein L484_006512 [Morus nota... 107 3e-21 ref|XP_007219022.1| hypothetical protein PRUPE_ppa004385mg [Prun... 107 3e-21 >ref|XP_007038731.1| ATP binding protein, putative [Theobroma cacao] gi|508775976|gb|EOY23232.1| ATP binding protein, putative [Theobroma cacao] Length = 542 Score = 125 bits (313), Expect = 2e-26 Identities = 67/120 (55%), Positives = 83/120 (69%), Gaps = 1/120 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK E+EER+KVV IL QELEKTK AL+ISN Sbjct: 410 LEVEAGKKEVEERDKVVVILKQELEKTKAALEISNGKLKLKEELAAAAMAAQAAAERSLQ 469 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLS-TATTYNRAKNVRR 3 ADS AAGLR+R+EEL+KQLEEAES++R+R K+RH+CWPWRALK++ T T NR ++V+R Sbjct: 470 LADSRAAGLRDRIEELTKQLEEAESKERSRRKVRHICWPWRALKMNITNNTNNRVQDVKR 529 >ref|XP_002272050.1| PREDICTED: uncharacterized protein At3g49055 [Vitis vinifera] gi|302143838|emb|CBI22699.3| unnamed protein product [Vitis vinifera] Length = 529 Score = 120 bits (302), Expect = 3e-25 Identities = 67/120 (55%), Positives = 81/120 (67%), Gaps = 1/120 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK+ +EER+KV IL QELEKTKTALDISN Sbjct: 400 LEVEAGKNVVEERDKVTLILKQELEKTKTALDISNGKLKLKEDLAAAAMAAQAAAERSLQ 459 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYN-RAKNVRR 3 ADS AA LRER+EEL++QLEEAES+DRNR ++RH+CWPWRALK++ A+ N R +VRR Sbjct: 460 LADSRAAELRERIEELTRQLEEAESRDRNRLRVRHVCWPWRALKVNPASNTNTRVGSVRR 519 >ref|XP_002513685.1| ATP binding protein, putative [Ricinus communis] gi|223547593|gb|EEF49088.1| ATP binding protein, putative [Ricinus communis] Length = 550 Score = 118 bits (295), Expect = 2e-24 Identities = 63/119 (52%), Positives = 80/119 (67%), Gaps = 1/119 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGKHEIEER+KVV I+ QEL++TK AL+ISN Sbjct: 418 LEVEAGKHEIEERDKVVFIMKQELDRTKAALEISNGKLKLKEDLASAAMAAQAASEKSLQ 477 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYN-RAKNVR 6 AD+ AAGL+ER+EEL KQLEEAES++R+R K+RH+CWPWR LK ++A N R ++VR Sbjct: 478 LADTRAAGLQERIEELRKQLEEAESKERSRRKVRHICWPWRTLKQNSANIANERVQSVR 536 >ref|XP_011048737.1| PREDICTED: uncharacterized protein At3g49055 [Populus euphratica] Length = 554 Score = 115 bits (289), Expect = 1e-23 Identities = 63/120 (52%), Positives = 80/120 (66%), Gaps = 1/120 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEA K IEER+K+V IL QELEKTK L+ISN Sbjct: 421 LEVEAAKKAIEERDKLVVILKQELEKTKANLEISNGKLKLKDELAVAAMAAQAAAERSLQ 480 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATT-YNRAKNVRR 3 ADS AAGLR+R+EEL++Q+EEAES++R +K+RH+CWPWRA+K +TA+T NR +NVRR Sbjct: 481 LADSRAAGLRQRIEELTRQVEEAESKERRCNKVRHICWPWRAIKAATASTANNRVQNVRR 540 >ref|XP_006421957.1| hypothetical protein CICLE_v10006475mg, partial [Citrus clementina] gi|557523830|gb|ESR35197.1| hypothetical protein CICLE_v10006475mg, partial [Citrus clementina] Length = 515 Score = 115 bits (289), Expect = 1e-23 Identities = 63/120 (52%), Positives = 78/120 (65%), Gaps = 1/120 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK EIEER+K+V IL QELEKTK AL++SN Sbjct: 383 LEVEAGKFEIEERDKLVFILKQELEKTKAALEVSNGKIKLKEELAAAAMAAQAAAETSLQ 442 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTA-TTYNRAKNVRR 3 AD AA LRER+EEL+KQLEEAES++++RHK+R++CWPWR +L+ A T R NVRR Sbjct: 443 LADGRAAELRERIEELTKQLEEAESKEQSRHKVRYICWPWRLFRLNAANNTDGRVPNVRR 502 >ref|XP_012440140.1| PREDICTED: uncharacterized protein At3g49055 isoform X1 [Gossypium raimondii] gi|763785693|gb|KJB52764.1| hypothetical protein B456_008G275800 [Gossypium raimondii] Length = 536 Score = 114 bits (286), Expect = 2e-23 Identities = 61/119 (51%), Positives = 77/119 (64%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK E+EER K+V IL QEL+KTKTAL+ISN Sbjct: 407 LEVEAGKKEVEEREKLVVILKQELDKTKTALEISNGKLKLKEELAAAAIAAQAAAERSVQ 466 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS A GLR+R+EEL+KQLEEAE ++R+R K+RH+CWPW LK++ T RA+N +R Sbjct: 467 LADSRAVGLRDRIEELTKQLEEAEGKERSRRKVRHICWPWEILKMNITNT--RAQNGKR 523 >gb|KHG09822.1| hypothetical protein F383_13172 [Gossypium arboreum] Length = 536 Score = 114 bits (286), Expect = 2e-23 Identities = 61/119 (51%), Positives = 77/119 (64%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK E+EER K+V IL QEL+KTKTAL+ISN Sbjct: 407 LEVEAGKKEVEEREKLVVILKQELDKTKTALEISNGKLKLKEELAAAAIAAQAAAERSVQ 466 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS A GLR+R+EEL+KQLEEAE ++R+R K+RH+CWPW LK++ T RA+N +R Sbjct: 467 LADSRAVGLRDRIEELTKQLEEAEGKERSRRKVRHICWPWEILKMNITNT--RAQNGKR 523 >ref|XP_008343766.1| PREDICTED: uncharacterized protein At3g49055-like [Malus domestica] Length = 533 Score = 114 bits (284), Expect = 4e-23 Identities = 64/119 (53%), Positives = 75/119 (63%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK EI ER KV++IL QELEKTK ALDISN Sbjct: 403 LEVEAGKTEIGEREKVIAILKQELEKTKAALDISNGKLKMKEELAXTAMAAQAAAERSLQ 462 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS A+GLR R+EEL+KQ+EEAES++RN ++RH+CWPWR LK S N K VRR Sbjct: 463 LADSRASGLRARIEELTKQVEEAESRERNNRRVRHICWPWRLLKASN-NAQNSIKPVRR 520 >ref|XP_008382404.1| PREDICTED: uncharacterized protein At3g49055-like [Malus domestica] Length = 258 Score = 114 bits (284), Expect = 4e-23 Identities = 64/119 (53%), Positives = 75/119 (63%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK EI ER KV++IL QELEKTK ALDISN Sbjct: 128 LEVEAGKTEIGEREKVIAILKQELEKTKAALDISNGKLKMKEELAXTAMAAQAAAERSLQ 187 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS A+GLR R+EEL+KQ+EEAES++RN ++RH+CWPWR LK S N K VRR Sbjct: 188 LADSRASGLRARIEELTKQVEEAESRERNNRRVRHICWPWRLLKASN-NAQNSIKPVRR 245 >ref|XP_002322480.2| hypothetical protein POPTR_0015s12920g [Populus trichocarpa] gi|550322604|gb|EEF06607.2| hypothetical protein POPTR_0015s12920g [Populus trichocarpa] Length = 536 Score = 114 bits (284), Expect = 4e-23 Identities = 62/120 (51%), Positives = 78/120 (65%), Gaps = 1/120 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEA K IEER K+V IL QELEKTK L+ISN Sbjct: 403 LEVEAAKKAIEEREKLVVILKQELEKTKANLEISNGKLKLKDELAVAAMAAQAAAERSLQ 462 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATT-YNRAKNVRR 3 ADS AAGLR+R+EEL++Q+EEAES++R +K+RH+CWPWRA+K +TA+T NR +N RR Sbjct: 463 LADSRAAGLRQRIEELTRQVEEAESKERRCNKVRHICWPWRAIKAATASTANNRVQNARR 522 >ref|XP_006490478.1| PREDICTED: uncharacterized protein At3g49055-like [Citrus sinensis] Length = 530 Score = 113 bits (283), Expect = 5e-23 Identities = 62/120 (51%), Positives = 77/120 (64%), Gaps = 1/120 (0%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEV AGK EIEER+K+V IL QELEKTK AL++SN Sbjct: 398 LEVAAGKFEIEERDKLVFILKQELEKTKAALEVSNGKIKLKEELAAAAMAAQAAAETSLQ 457 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTA-TTYNRAKNVRR 3 AD AA LRER+EEL+KQLEEAES++++RHK+R++CWPWR +L+ A T R NVRR Sbjct: 458 LADGRAAELRERIEELTKQLEEAESKEQSRHKVRYICWPWRLFRLNAANNTDGRVPNVRR 517 >ref|XP_004309199.1| PREDICTED: uncharacterized protein At3g49055 [Fragaria vesca subsp. vesca] Length = 523 Score = 113 bits (282), Expect = 6e-23 Identities = 57/108 (52%), Positives = 74/108 (68%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK+EIEER K+++IL QELEKT+ ALDISN Sbjct: 396 LEVEAGKNEIEEREKLIAILKQELEKTRAALDISNGKLKLKEELAANAMAAHAAAEKSLQ 455 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTA 36 ADS AAGLR R+EELS+Q+EEAES++R+ ++RH+CWPWR+LK++ A Sbjct: 456 LADSRAAGLRNRIEELSRQVEEAESRERHSRRVRHICWPWRSLKVNPA 503 >ref|XP_009340052.1| PREDICTED: uncharacterized protein At3g49055-like [Pyrus x bretschneideri] Length = 533 Score = 112 bits (281), Expect = 8e-23 Identities = 62/119 (52%), Positives = 74/119 (62%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK EI ER KV++IL QELEKTK ALDISN Sbjct: 403 LEVEAGKTEIGEREKVIAILKQELEKTKAALDISNGKLKMKEELAVTAMAAQAAAERSLQ 462 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS A+GLR R+EEL+KQ+EEAES++RN ++RH+CWPWR LK S N K V+R Sbjct: 463 LADSRASGLRARIEELTKQVEEAESRERNNRRVRHICWPWRLLKASN-NAQNSIKPVKR 520 >ref|XP_008376601.1| PREDICTED: uncharacterized protein At3g49055-like [Malus domestica] Length = 532 Score = 112 bits (280), Expect = 1e-22 Identities = 61/119 (51%), Positives = 74/119 (62%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK EI ER KV++IL QELEKTKTALD+SN Sbjct: 398 LEVEAGKTEIGEREKVIAILKQELEKTKTALDVSNAKLKLKEELAATAMAAQAAAERSLQ 457 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 DS +GLR+R+EELSKQ+EEAES++RN ++R +CWPWR LK + T NR VRR Sbjct: 458 LTDSRTSGLRKRIEELSKQVEEAESRERNNRRVRCICWPWRPLKAAN-TAQNRIGPVRR 515 >ref|XP_010060758.1| PREDICTED: uncharacterized protein At3g49055 isoform X1 [Eucalyptus grandis] gi|629126253|gb|KCW90678.1| hypothetical protein EUGRSUZ_A02769 [Eucalyptus grandis] Length = 541 Score = 112 bits (279), Expect = 1e-22 Identities = 64/121 (52%), Positives = 75/121 (61%), Gaps = 2/121 (1%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEV AG HEIEERNKVV IL QELEKTK ALDISN Sbjct: 408 LEVVAGNHEIEERNKVVIILKQELEKTKAALDISNGKLKLKEDLVTTAMAAQEAAERSLQ 467 Query: 179 XADSTAAGLRERVEELSKQLEEAE-SQDRNRHKIRHLCWPWRALKLSTATTYN-RAKNVR 6 AD AAGLRER+EEL++QLE A+ +++NR ++RH CWPWR LK++ A N R NVR Sbjct: 468 LADRRAAGLRERIEELTRQLEGADGKENKNRRRVRHFCWPWRILKMNPANPANARFPNVR 527 Query: 5 R 3 R Sbjct: 528 R 528 >gb|KCW90677.1| hypothetical protein EUGRSUZ_A02769 [Eucalyptus grandis] Length = 548 Score = 112 bits (279), Expect = 1e-22 Identities = 64/121 (52%), Positives = 75/121 (61%), Gaps = 2/121 (1%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEV AG HEIEERNKVV IL QELEKTK ALDISN Sbjct: 415 LEVVAGNHEIEERNKVVIILKQELEKTKAALDISNGKLKLKEDLVTTAMAAQEAAERSLQ 474 Query: 179 XADSTAAGLRERVEELSKQLEEAE-SQDRNRHKIRHLCWPWRALKLSTATTYN-RAKNVR 6 AD AAGLRER+EEL++QLE A+ +++NR ++RH CWPWR LK++ A N R NVR Sbjct: 475 LADRRAAGLRERIEELTRQLEGADGKENKNRRRVRHFCWPWRILKMNPANPANARFPNVR 534 Query: 5 R 3 R Sbjct: 535 R 535 >ref|XP_008234212.1| PREDICTED: uncharacterized protein At3g49055 [Prunus mume] Length = 539 Score = 111 bits (278), Expect = 2e-22 Identities = 62/119 (52%), Positives = 75/119 (63%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK+EI ER KV++IL QELEKTK ALDISN Sbjct: 409 LEVEAGKNEIGEREKVIAILKQELEKTKAALDISNGKLKLKEELAATAMAAQAAAERSLQ 468 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS AAGLR R+EELS+Q+EEAES++RN ++R +CWPWR LK++ NR VRR Sbjct: 469 LADSRAAGLRLRIEELSRQVEEAESRERNNRRVRRICWPWRPLKVN-PNAQNRVGAVRR 526 >ref|XP_009342947.1| PREDICTED: uncharacterized protein At3g49055-like [Pyrus x bretschneideri] Length = 531 Score = 110 bits (276), Expect = 3e-22 Identities = 59/119 (49%), Positives = 74/119 (62%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK EI ER KV++IL QELEKTK ALD+SN Sbjct: 401 LEVEAGKTEIGEREKVITILKQELEKTKAALDMSNGKLKLKEELAATAMAAQAAAERSLQ 460 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 DS +GLR+R+EELSKQ+EEAES++R+ ++RH+CWPWR LK + NR + VRR Sbjct: 461 LTDSRTSGLRKRIEELSKQVEEAESRERSNRRVRHICWPWRPLKAAN-NAQNRIEPVRR 518 >ref|XP_010108356.1| hypothetical protein L484_006512 [Morus notabilis] gi|587932075|gb|EXC19147.1| hypothetical protein L484_006512 [Morus notabilis] Length = 480 Score = 107 bits (268), Expect = 3e-21 Identities = 57/104 (54%), Positives = 68/104 (65%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK I ER+KV++IL QELEK K ALDISN Sbjct: 373 LEVEAGKLVIGERDKVIAILKQELEKNKAALDISNGKLKLKEELAAAAMAAQAAAEKSLE 432 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALK 48 ADS A GLRER+EELS+QLEEAES++R+ ++RH+CWPWRALK Sbjct: 433 LADSRAVGLRERIEELSRQLEEAESRERSSRRVRHICWPWRALK 476 >ref|XP_007219022.1| hypothetical protein PRUPE_ppa004385mg [Prunus persica] gi|462415484|gb|EMJ20221.1| hypothetical protein PRUPE_ppa004385mg [Prunus persica] Length = 513 Score = 107 bits (268), Expect = 3e-21 Identities = 60/119 (50%), Positives = 74/119 (62%) Frame = -1 Query: 359 LEVEAGKHEIEERNKVVSILSQELEKTKTALDISNRXXXXXXXXXXXXXXXXXXXXXXXX 180 LEVEAGK+EI ER KV++IL QELEKTK ALD+SN Sbjct: 383 LEVEAGKNEIGEREKVIAILKQELEKTKAALDMSNGKLKLKEELAATAMAAQAAAERSLQ 442 Query: 179 XADSTAAGLRERVEELSKQLEEAESQDRNRHKIRHLCWPWRALKLSTATTYNRAKNVRR 3 ADS AAGLR R+EELS+Q+EEAE ++RN ++R +CWPWR LK++ NR VRR Sbjct: 443 LADSRAAGLRLRIEELSRQVEEAEGRERNNRRVRCICWPWRPLKVN-PNAQNRVGAVRR 500