BLASTX nr result
ID: Ziziphus21_contig00019883
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ziziphus21_contig00019883
(1329 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002510213.1| Protein COQ10 B, mitochondrial precursor, pu... 375 e-101
ref|XP_007017233.1| Polyketide cyclase / dehydrase and lipid tra... 374 e-100
ref|XP_006434763.1| hypothetical protein CICLE_v10002229mg [Citr... 373 e-100
gb|KDO84163.1| hypothetical protein CISIN_1g025224mg [Citrus sin... 370 1e-99
ref|XP_006473323.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 369 3e-99
ref|XP_012445154.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 367 1e-98
gb|KHG17257.1| Coenzyme Q-binding COQ10 B, mitochondrial [Gossyp... 367 2e-98
ref|XP_012071659.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 365 5e-98
ref|XP_008452548.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 360 1e-96
gb|KGN55193.1| hypothetical protein Csa_4G639810 [Cucumis sativus] 359 3e-96
ref|XP_008351995.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 359 3e-96
ref|XP_011654108.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 358 4e-96
ref|XP_008378044.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 358 8e-96
ref|XP_004291106.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 357 1e-95
ref|XP_007227558.1| hypothetical protein PRUPE_ppa010640mg [Prun... 357 1e-95
ref|XP_012451993.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 357 1e-95
ref|XP_008220227.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 357 2e-95
gb|KHG15657.1| Coenzyme Q-binding COQ10, mitochondrial [Gossypiu... 354 8e-95
ref|XP_003523942.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 354 8e-95
ref|XP_010059931.1| PREDICTED: coenzyme Q-binding protein COQ10 ... 353 2e-94
>ref|XP_002510213.1| Protein COQ10 B, mitochondrial precursor, putative [Ricinus communis]
gi|223550914|gb|EEF52400.1| Protein COQ10 B,
mitochondrial precursor, putative [Ricinus communis]
Length = 252
Score = 375 bits (962), Expect = e-101
Identities = 191/254 (75%), Positives = 215/254 (84%), Gaps = 2/254 (0%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF STTKA+ L + ++ + L+R S K SK HQ RC+ S I G+ T +VR+LI
Sbjct: 1 MPPFMSTTKALRFLTTNKNVAKGLIR-SPKNPTSKYNHQIRCYDS-IMGMSTPTVRELID 58
Query: 866 SANQ--DVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVA 693
+ N D +S + +N Q+R+FLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVA
Sbjct: 59 NYNNNNDNNSCTIGRLYNNNSQKRQFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVA 118
Query: 692 AVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDS 513
AVDLYHGFVPWCQRSD++RH+PDGSFDAELEIGFKFLVESY+SHVEL RPK +KTTVSDS
Sbjct: 119 AVDLYHGFVPWCQRSDIIRHHPDGSFDAELEIGFKFLVESYISHVELKRPKSIKTTVSDS 178
Query: 512 TLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCR 333
TLFDHLINIWEFNPGPVPGTC+LYFLVDFKFQSPLYRQVAS+FFKEVVSRLVGSFS+RCR
Sbjct: 179 TLFDHLINIWEFNPGPVPGTCDLYFLVDFKFQSPLYRQVASVFFKEVVSRLVGSFSERCR 238
Query: 332 LVYGPAVPVLENSY 291
LVYGP V VLENSY
Sbjct: 239 LVYGPGVSVLENSY 252
>ref|XP_007017233.1| Polyketide cyclase / dehydrase and lipid transport protein [Theobroma
cacao] gi|508722561|gb|EOY14458.1| Polyketide cyclase /
dehydrase and lipid transport protein [Theobroma cacao]
Length = 255
Score = 374 bits (960), Expect = e-100
Identities = 187/256 (73%), Positives = 214/256 (83%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF ST+KAV SL+SRR+G + VR+ S++ Q R +FS IAGI+T SV LIG
Sbjct: 1 MPPFLSTSKAVGSLISRRNGISHFVRHVKSGGHSREFDQFR-WFSNIAGIKTPSVHPLIG 59
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
S ++ + + D +Q R FLGCGDGEEGGVLS+VYEER V+GYSPEQLFDVVAAV
Sbjct: 60 SRKEESNFSFKSLCDKYALQSRPFLGCGDGEEGGVLSRVYEERIVMGYSPEQLFDVVAAV 119
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRSD+++ PDGSFDAELEIGFKFLVESYVSHVEL R K +K+T S+S+L
Sbjct: 120 DLYHGFVPWCQRSDIIKRYPDGSFDAELEIGFKFLVESYVSHVELSRAKFVKSTASESSL 179
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEF+PGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLV SFS+RCRL+
Sbjct: 180 FDHLINIWEFSPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVSSFSERCRLI 239
Query: 326 YGPAVPVLENSYGQRA 279
YGP VPVLENSYG+RA
Sbjct: 240 YGPGVPVLENSYGERA 255
>ref|XP_006434763.1| hypothetical protein CICLE_v10002229mg [Citrus clementina]
gi|557536885|gb|ESR48003.1| hypothetical protein
CICLE_v10002229mg [Citrus clementina]
Length = 256
Score = 373 bits (957), Expect = e-100
Identities = 189/256 (73%), Positives = 213/256 (83%), Gaps = 1/256 (0%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF +KA+ SL+SR +G R+L+ + + +KL+Q R GI GIET SV +LIG
Sbjct: 1 MPPFMPVSKALGSLVSRTTGSRHLISSAKSDGQLRKLNQFRRL-CGITGIETSSVHKLIG 59
Query: 866 SANQDVSSLSGAYFDVSNV-QRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAA 690
+ SSL+ + NV QRRRFLG GDGEEG VLSKVYEERRVLGYSPEQLFDVVAA
Sbjct: 60 THQNISSSLANFCQNNGNVLQRRRFLGVGDGEEGDVLSKVYEERRVLGYSPEQLFDVVAA 119
Query: 689 VDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDST 510
VDLYHGFVPWCQRS++L+HNPDGSFDAELEIGFKFLVESYVSHVEL+RPK +KTT SDS+
Sbjct: 120 VDLYHGFVPWCQRSEILKHNPDGSFDAELEIGFKFLVESYVSHVELNRPKFVKTTASDSS 179
Query: 509 LFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRL 330
LFDHLINIWEFNPGP PGTCNLYFLVDFKF SPLYRQ ASMFFKEVVSRLVGSFS+RCRL
Sbjct: 180 LFDHLINIWEFNPGPSPGTCNLYFLVDFKFHSPLYRQAASMFFKEVVSRLVGSFSERCRL 239
Query: 329 VYGPAVPVLENSYGQR 282
+YGP P+LEN+Y QR
Sbjct: 240 IYGPEAPILENTYEQR 255
>gb|KDO84163.1| hypothetical protein CISIN_1g025224mg [Citrus sinensis]
Length = 256
Score = 370 bits (951), Expect = 1e-99
Identities = 188/256 (73%), Positives = 212/256 (82%), Gaps = 1/256 (0%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF +KA+ SL+SR +G R+L+ + + + L+Q R GI GIET SV +LIG
Sbjct: 1 MPPFMPVSKALGSLVSRTTGSRHLISSAKSDGQLRMLNQFRRL-CGITGIETSSVHKLIG 59
Query: 866 SANQDVSSLSGAYFDVSNV-QRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAA 690
+ SSL+ + NV QRRRFLG GDGEEG VLSKVYEERRVLGYSPEQLFDVVAA
Sbjct: 60 AHQNISSSLANFCQNNGNVLQRRRFLGVGDGEEGDVLSKVYEERRVLGYSPEQLFDVVAA 119
Query: 689 VDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDST 510
VDLYHGFVPWCQRS++L+HNPDGSFDAELEIGFKFLVESYVSHVEL+RPK +KTT SDS+
Sbjct: 120 VDLYHGFVPWCQRSEILKHNPDGSFDAELEIGFKFLVESYVSHVELNRPKFVKTTASDSS 179
Query: 509 LFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRL 330
LFDHLINIWEFNPGP PGTCNLYFLVDFKF SPLYRQ ASMFFKEVVSRLVGSFS+RCRL
Sbjct: 180 LFDHLINIWEFNPGPSPGTCNLYFLVDFKFHSPLYRQAASMFFKEVVSRLVGSFSERCRL 239
Query: 329 VYGPAVPVLENSYGQR 282
+YGP P+LEN+Y QR
Sbjct: 240 IYGPEAPILENTYEQR 255
>ref|XP_006473323.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog A,
mitochondrial-like [Citrus sinensis]
Length = 256
Score = 369 bits (948), Expect = 3e-99
Identities = 188/256 (73%), Positives = 211/256 (82%), Gaps = 1/256 (0%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF +KA+ SL+SR +G R+L+ + + L+Q R GI GIET SV +LIG
Sbjct: 1 MPPFMPVSKALGSLVSRTTGSRHLISSVKSDGQLRMLNQFRRL-CGITGIETSSVHKLIG 59
Query: 866 SANQDVSSLSGAYFDVSNV-QRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAA 690
+ SSL+ + NV QRRRFLG GDGEEG VLSKVYEERRVLGYSPEQLFDVVAA
Sbjct: 60 THQNISSSLANFCQNNGNVLQRRRFLGVGDGEEGDVLSKVYEERRVLGYSPEQLFDVVAA 119
Query: 689 VDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDST 510
VDLYHGFVPWCQRS++L+HNPDGSFDAELEIGFKFLVESYVSHVEL+RPK +KTT SDS+
Sbjct: 120 VDLYHGFVPWCQRSEILKHNPDGSFDAELEIGFKFLVESYVSHVELNRPKFVKTTASDSS 179
Query: 509 LFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRL 330
LFDHLINIWEFNPGP PGTCNLYFLVDFKF SPLYRQ ASMFFKEVVSRLVGSFS+RCRL
Sbjct: 180 LFDHLINIWEFNPGPSPGTCNLYFLVDFKFHSPLYRQAASMFFKEVVSRLVGSFSERCRL 239
Query: 329 VYGPAVPVLENSYGQR 282
+YGP P+LEN+Y QR
Sbjct: 240 IYGPEAPILENTYEQR 255
>ref|XP_012445154.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog B, mitochondrial
[Gossypium raimondii] gi|763791483|gb|KJB58479.1|
hypothetical protein B456_009G212100 [Gossypium
raimondii] gi|763791485|gb|KJB58481.1| hypothetical
protein B456_009G212100 [Gossypium raimondii]
Length = 255
Score = 367 bits (942), Expect = 1e-98
Identities = 181/255 (70%), Positives = 210/255 (82%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF ST+KAV S++SRR+ VR++ R+ Q R + + I+GI+ V L+G
Sbjct: 1 MPPFLSTSKAVGSVISRRNVINQFVRHAKNGGRTGNFDQIR-WVTSISGIQMPPVHPLLG 59
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
S D +F+ +QRR+FLGCGDGEEGGVLSKVYEER V+GYSPEQLFDVVAAV
Sbjct: 60 SRKVDSVVPLNYFFNHYTLQRRQFLGCGDGEEGGVLSKVYEERIVMGYSPEQLFDVVAAV 119
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRSDV++H PDGSFDAELEIGFKFLVESYVSHVEL RPK +K+T S S+L
Sbjct: 120 DLYHGFVPWCQRSDVIKHYPDGSFDAELEIGFKFLVESYVSHVELSRPKFVKSTASQSSL 179
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEFNPGPVPG+C+LYFLVDFKFQSPLYRQVASMFFKEVVSR++ SFS+RCRL+
Sbjct: 180 FDHLINIWEFNPGPVPGSCSLYFLVDFKFQSPLYRQVASMFFKEVVSRMISSFSERCRLI 239
Query: 326 YGPAVPVLENSYGQR 282
YGP VPVLENSYG+R
Sbjct: 240 YGPGVPVLENSYGER 254
>gb|KHG17257.1| Coenzyme Q-binding COQ10 B, mitochondrial [Gossypium arboreum]
Length = 255
Score = 367 bits (941), Expect = 2e-98
Identities = 181/255 (70%), Positives = 210/255 (82%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF ST+KAV S++SRR+ V ++ R+ Q R + + I+GI+ SV ++G
Sbjct: 1 MPPFLSTSKAVGSVISRRNVINQFVGHAKNGGRTGNFDQIR-WVTSISGIQMPSVHPVLG 59
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
S D +F+ QRR+FLGCGDGEEGGVLSKVYEER V+GYSPEQLFDVVAAV
Sbjct: 60 SRKVDSIVPLNYFFNHYTFQRRQFLGCGDGEEGGVLSKVYEERIVMGYSPEQLFDVVAAV 119
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRSDV++H PDGSFDAELEIGFKFLVESYVSHVEL RPK +K+T S+S+L
Sbjct: 120 DLYHGFVPWCQRSDVIKHYPDGSFDAELEIGFKFLVESYVSHVELSRPKFVKSTASESSL 179
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEFNPGPVPGTC+LYFLVDFKFQSPLYRQVASMFFKEVVSR++ SFS+RCRL+
Sbjct: 180 FDHLINIWEFNPGPVPGTCSLYFLVDFKFQSPLYRQVASMFFKEVVSRMISSFSERCRLI 239
Query: 326 YGPAVPVLENSYGQR 282
YGP VPVLENSYG+R
Sbjct: 240 YGPGVPVLENSYGER 254
>ref|XP_012071659.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Jatropha curcas] gi|643730996|gb|KDP38334.1|
hypothetical protein JCGZ_04259 [Jatropha curcas]
Length = 252
Score = 365 bits (937), Expect = 5e-98
Identities = 180/256 (70%), Positives = 211/256 (82%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF S++KAV SL++ ++ L+R + + + + RC+ S I GI SV ++I
Sbjct: 1 MPPFMSSSKAVRSLITSKNVAEYLIR---SAKNNSRFDRIRCY-SSIVGISAPSVGEVID 56
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
+L Y + S Q+R+FLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV
Sbjct: 57 RKKNFSFTLGSLYNNASAAQKRQFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 116
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRS++L++ PDGSFDAELEIGFKFLVESY+SHVEL+RPK +KTTVSDST+
Sbjct: 117 DLYHGFVPWCQRSEILKYYPDGSFDAELEIGFKFLVESYISHVELNRPKFIKTTVSDSTI 176
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEFNPGPVPGTC+L+F VDFKFQSPLYRQVASMFFKEVVSRLVGSFS+RCRL+
Sbjct: 177 FDHLINIWEFNPGPVPGTCDLHFFVDFKFQSPLYRQVASMFFKEVVSRLVGSFSERCRLI 236
Query: 326 YGPAVPVLENSYGQRA 279
YGP VPVLE SY Q+A
Sbjct: 237 YGPGVPVLEKSYEQKA 252
>ref|XP_008452548.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Cucumis melo] gi|659103266|ref|XP_008452550.1|
PREDICTED: coenzyme Q-binding protein COQ10 homolog,
mitochondrial [Cucumis melo]
gi|659103268|ref|XP_008452551.1| PREDICTED: coenzyme
Q-binding protein COQ10 homolog, mitochondrial [Cucumis
melo]
Length = 252
Score = 360 bits (925), Expect = 1e-96
Identities = 180/259 (69%), Positives = 209/259 (80%), Gaps = 3/259 (1%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYS---GKVERSKKLHQSRCFFSGIAGIETGSVRQ 876
MPPF ST+K + SL+SRR G R +RY+ + + + + RC I G+ET S+
Sbjct: 1 MPPFLSTSKILESLISRRGGIRRAIRYARCNAEPWKYRNVRHLRC----IPGVETLSIHG 56
Query: 875 LIGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVV 696
L +QD S + D+S VQ RRFLGCGDG E GVLSK+YEERRV+GYSPEQLFDVV
Sbjct: 57 LF---DQDRKVFSENFRDISAVQSRRFLGCGDGAETGVLSKIYEERRVMGYSPEQLFDVV 113
Query: 695 AAVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSD 516
AAVDLYH FVPWCQRS+VL+ PDGSFDAELEIGFKFLVESY+SHVE++RPK +K+TVS
Sbjct: 114 AAVDLYHDFVPWCQRSEVLKKYPDGSFDAELEIGFKFLVESYISHVEMNRPKSIKSTVSR 173
Query: 515 STLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRC 336
S LFDHLIN WEFNPGPVPGTCNLYFLVDFKFQSPLYRQVAS+FFKEVVS+LVGSF++RC
Sbjct: 174 SALFDHLINTWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASVFFKEVVSKLVGSFNERC 233
Query: 335 RLVYGPAVPVLENSYGQRA 279
RL+YGP VPVLENSY +RA
Sbjct: 234 RLIYGPGVPVLENSYSERA 252
>gb|KGN55193.1| hypothetical protein Csa_4G639810 [Cucumis sativus]
Length = 667
Score = 359 bits (921), Expect = 3e-96
Identities = 177/260 (68%), Positives = 209/260 (80%), Gaps = 3/260 (1%)
Frame = -2
Query: 1049 SMPPFTSTTKAVLSLLSRRSGFRNLVRYS---GKVERSKKLHQSRCFFSGIAGIETGSVR 879
+MPPF S +K + SL+SRR G + ++Y+ + + + + RC I G+ET S+
Sbjct: 415 AMPPFLSNSKVLESLISRRGGIKRAIKYARCNAEPWKYRNVRHLRC----IPGVETSSIH 470
Query: 878 QLIGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDV 699
L +QD S + D+S VQ RRFLGCGDG E GVLSK+YEERRV+GYSPEQLFDV
Sbjct: 471 GLF---DQDRKMFSENFRDISAVQSRRFLGCGDGAETGVLSKIYEERRVMGYSPEQLFDV 527
Query: 698 VAAVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVS 519
VAAVDLYH FVPWCQRS+VL+ PDGSFDAELEIGFKFLVESY+SHVE++RPK +K+TVS
Sbjct: 528 VAAVDLYHDFVPWCQRSEVLKKYPDGSFDAELEIGFKFLVESYISHVEMNRPKSVKSTVS 587
Query: 518 DSTLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDR 339
S LFDHLIN WEFNPGPVPGTCNLYFLVDFKFQSPLYRQVAS+FFKEVVS+LVGSF++R
Sbjct: 588 RSALFDHLINTWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASVFFKEVVSKLVGSFNER 647
Query: 338 CRLVYGPAVPVLENSYGQRA 279
CRL+YGP VPVLENSY +RA
Sbjct: 648 CRLIYGPGVPVLENSYSERA 667
>ref|XP_008351995.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog,
mitochondrial-like [Malus domestica]
gi|658063501|ref|XP_008367671.1| PREDICTED: coenzyme
Q-binding protein COQ10 homolog, mitochondrial-like
[Malus domestica]
Length = 243
Score = 359 bits (921), Expect = 3e-96
Identities = 188/260 (72%), Positives = 213/260 (81%), Gaps = 4/260 (1%)
Frame = -2
Query: 1046 MPPFTSTT--KAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIET--GSVR 879
MPPF S+T KAV SLLSRR G R+LVR S+KL RCF S I GIET SV
Sbjct: 1 MPPFWSSTTSKAVGSLLSRRIGPRHLVR------SSRKLDPIRCF-SSIVGIETPSSSVH 53
Query: 878 QLIGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDV 699
+ + D + S RR+FLGCGDGEEGGVLSKVYEERRV+GYSPEQL++V
Sbjct: 54 KWTTDSQXDFNYRS----------RRQFLGCGDGEEGGVLSKVYEERRVMGYSPEQLYBV 103
Query: 698 VAAVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVS 519
VAAVDLYHGFVPWCQRSD+++ PDGSFDAELEIGFKFLVESY SHVEL+RPKR+K+TVS
Sbjct: 104 VAAVDLYHGFVPWCQRSDIIKAYPDGSFDAELEIGFKFLVESYTSHVELERPKRIKSTVS 163
Query: 518 DSTLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDR 339
+S+LFDHLINIWEFNPGPVPG+C+LYFLVDFKFQSPLYRQVAS+FFKEVVS+LVGSF++R
Sbjct: 164 NSSLFDHLINIWEFNPGPVPGSCSLYFLVDFKFQSPLYRQVASVFFKEVVSKLVGSFNER 223
Query: 338 CRLVYGPAVPVLENSYGQRA 279
CRL+YGP V VLENSYGQRA
Sbjct: 224 CRLIYGPGVRVLENSYGQRA 243
>ref|XP_011654108.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Cucumis sativus]
Length = 252
Score = 358 bits (920), Expect = 4e-96
Identities = 177/259 (68%), Positives = 208/259 (80%), Gaps = 3/259 (1%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYS---GKVERSKKLHQSRCFFSGIAGIETGSVRQ 876
MPPF S +K + SL+SRR G + ++Y+ + + + + RC I G+ET S+
Sbjct: 1 MPPFLSNSKVLESLISRRGGIKRAIKYARCNAEPWKYRNVRHLRC----IPGVETSSIHG 56
Query: 875 LIGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVV 696
L +QD S + D+S VQ RRFLGCGDG E GVLSK+YEERRV+GYSPEQLFDVV
Sbjct: 57 LF---DQDRKMFSENFRDISAVQSRRFLGCGDGAETGVLSKIYEERRVMGYSPEQLFDVV 113
Query: 695 AAVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSD 516
AAVDLYH FVPWCQRS+VL+ PDGSFDAELEIGFKFLVESY+SHVE++RPK +K+TVS
Sbjct: 114 AAVDLYHDFVPWCQRSEVLKKYPDGSFDAELEIGFKFLVESYISHVEMNRPKSVKSTVSR 173
Query: 515 STLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRC 336
S LFDHLIN WEFNPGPVPGTCNLYFLVDFKFQSPLYRQVAS+FFKEVVS+LVGSF++RC
Sbjct: 174 SALFDHLINTWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASVFFKEVVSKLVGSFNERC 233
Query: 335 RLVYGPAVPVLENSYGQRA 279
RL+YGP VPVLENSY +RA
Sbjct: 234 RLIYGPGVPVLENSYSERA 252
>ref|XP_008378044.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Malus domestica]
Length = 243
Score = 358 bits (918), Expect = 8e-96
Identities = 188/258 (72%), Positives = 211/258 (81%), Gaps = 2/258 (0%)
Frame = -2
Query: 1046 MPPFTSTT--KAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQL 873
MPPF S+T KAV SL SR+ G R LVR +K Q RCF S IAGIET S
Sbjct: 1 MPPFWSSTTSKAVRSLHSRKIGPRYLVR------SGRKFDQIRCF-SSIAGIETPSSSVH 53
Query: 872 IGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVA 693
+A+ + D + RR+FLGCGDGEEGGVLSKVYEERRVLGYSPEQL+DVVA
Sbjct: 54 KWTADSQL--------DFNYRSRRQFLGCGDGEEGGVLSKVYEERRVLGYSPEQLYDVVA 105
Query: 692 AVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDS 513
AVDLYHGFVPWCQRS +++ PDGSFDAELEIGFKFLVESYVSHVEL+RPKR+KTTVS+S
Sbjct: 106 AVDLYHGFVPWCQRSAIIKTYPDGSFDAELEIGFKFLVESYVSHVELERPKRIKTTVSNS 165
Query: 512 TLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCR 333
+LFDHLINIWEFNPGPVPG+C+LYFLVDFKFQSPLYRQVAS+FFKEVVS+LVGSF++RCR
Sbjct: 166 SLFDHLINIWEFNPGPVPGSCSLYFLVDFKFQSPLYRQVASVFFKEVVSKLVGSFNERCR 225
Query: 332 LVYGPAVPVLENSYGQRA 279
L+YGP V VLENSYGQRA
Sbjct: 226 LIYGPGVRVLENSYGQRA 243
>ref|XP_004291106.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Fragaria vesca subsp. vesca]
Length = 251
Score = 357 bits (917), Expect = 1e-95
Identities = 186/257 (72%), Positives = 207/257 (80%), Gaps = 2/257 (0%)
Frame = -2
Query: 1046 MPPFTSTT--KAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQL 873
MPPF S+T KAV SLLSR G ++ ++ + S IAGIET SVR+
Sbjct: 1 MPPFWSSTASKAVGSLLSRGHGAKHHHHLYTSARNFNRIRR----LSSIAGIETPSVRKW 56
Query: 872 IGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVA 693
G VS+ S + + QRRRFLGCGDGEEGGVLSKVYEE+RVLGYSPEQLFDVVA
Sbjct: 57 TGDG---VSNTSRVLTNPFHEQRRRFLGCGDGEEGGVLSKVYEEKRVLGYSPEQLFDVVA 113
Query: 692 AVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDS 513
AVDLYHGFVPWCQRS+++ DGSFDAELEIGFKFLVESYVSHVEL+RPKR+KTTVS+S
Sbjct: 114 AVDLYHGFVPWCQRSEIVHTYADGSFDAELEIGFKFLVESYVSHVELERPKRIKTTVSNS 173
Query: 512 TLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCR 333
TLFDHLINIWEFNPGPVPG CNLYFLVDFKFQSPLYRQVAS+FFKEVVS+LVGSF+DRCR
Sbjct: 174 TLFDHLINIWEFNPGPVPGACNLYFLVDFKFQSPLYRQVASVFFKEVVSKLVGSFNDRCR 233
Query: 332 LVYGPAVPVLENSYGQR 282
L+YGP V V ENSYGQR
Sbjct: 234 LIYGPGVQVHENSYGQR 250
>ref|XP_007227558.1| hypothetical protein PRUPE_ppa010640mg [Prunus persica]
gi|462424494|gb|EMJ28757.1| hypothetical protein
PRUPE_ppa010640mg [Prunus persica]
Length = 242
Score = 357 bits (917), Expect = 1e-95
Identities = 185/259 (71%), Positives = 213/259 (82%), Gaps = 3/259 (1%)
Frame = -2
Query: 1046 MPPFTSTT--KAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIET-GSVRQ 876
MPPF S+T KAV SLLSRR G R+L+R +K RCF S IAGIET S+ +
Sbjct: 1 MPPFWSSTTSKAVGSLLSRRIGPRHLIR------SGRKYDPVRCF-SNIAGIETPSSIHK 53
Query: 875 LIGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVV 696
G + D + S RR+FLGCGDGEEGGVLSKVYEE+RVLGYSPEQLFDVV
Sbjct: 54 WTGDSRLDYNYRS----------RRQFLGCGDGEEGGVLSKVYEEKRVLGYSPEQLFDVV 103
Query: 695 AAVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSD 516
AAVDLYHGFVPWCQRSD+++ PDGSFDAELEIGFKFLVESYVSHVEL+R KR+KTTVS+
Sbjct: 104 AAVDLYHGFVPWCQRSDIIKTFPDGSFDAELEIGFKFLVESYVSHVELERAKRIKTTVSN 163
Query: 515 STLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRC 336
S+LFDHLINIWEFNPGPVPG+CNLYFLVDFKF+SP+YRQVAS+FFKEVVS+LVGSF++RC
Sbjct: 164 SSLFDHLINIWEFNPGPVPGSCNLYFLVDFKFRSPIYRQVASVFFKEVVSKLVGSFNERC 223
Query: 335 RLVYGPAVPVLENSYGQRA 279
RL+YGP VP+ E++YGQRA
Sbjct: 224 RLIYGPGVPIHEHTYGQRA 242
>ref|XP_012451993.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog,
mitochondrial-like [Gossypium raimondii]
gi|763796880|gb|KJB63835.1| hypothetical protein
B456_010G019900 [Gossypium raimondii]
Length = 255
Score = 357 bits (916), Expect = 1e-95
Identities = 180/255 (70%), Positives = 207/255 (81%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF+ST+KAV SL+SRR + VR + S+ Q RC S I+G ET S L+
Sbjct: 1 MPPFSSTSKAVGSLISRRIRITHFVRNANNGGCSRSSDQIRCV-STISGTETLSSHPLLA 59
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
S +D S D +Q+RRFLGC DGEEGG LSKV+EE+ ++GYSPEQLFDVVAAV
Sbjct: 60 SPKEDSFVPSNKISDRYTLQKRRFLGCSDGEEGGALSKVHEEKIIVGYSPEQLFDVVAAV 119
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRSD+++ P+GSFDAELEIGFKFLVESYVS VEL RPK +K+TVS+STL
Sbjct: 120 DLYHGFVPWCQRSDIVKQYPNGSFDAELEIGFKFLVESYVSRVELSRPKFVKSTVSESTL 179
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEFNPGPV GTCNL+FLVDFKFQSPLYRQVASMFFKEVVSRLV SFS+RCRL+
Sbjct: 180 FDHLINIWEFNPGPVLGTCNLHFLVDFKFQSPLYRQVASMFFKEVVSRLVSSFSERCRLI 239
Query: 326 YGPAVPVLENSYGQR 282
YGPA+PVLENSYG+R
Sbjct: 240 YGPAIPVLENSYGER 254
>ref|XP_008220227.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Prunus mume]
Length = 242
Score = 357 bits (915), Expect = 2e-95
Identities = 184/259 (71%), Positives = 213/259 (82%), Gaps = 3/259 (1%)
Frame = -2
Query: 1046 MPPFTSTT--KAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIET-GSVRQ 876
MPPF S+T KAV SLLSRR G R+L+R +K RCF S IAGIET S+ +
Sbjct: 1 MPPFWSSTTSKAVGSLLSRRIGPRHLIR------SGRKYDPVRCF-SSIAGIETPSSIHK 53
Query: 875 LIGSANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVV 696
G + D + S RR+FLGCGDGEEGGVLS+VYEE+RVLGYSPEQLFDVV
Sbjct: 54 WTGDSRLDYNYRS----------RRQFLGCGDGEEGGVLSRVYEEKRVLGYSPEQLFDVV 103
Query: 695 AAVDLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSD 516
AAVDLYHGFVPWC+RSD+++ PDGSFDAELEIGFKFLVESYVSHVEL+R KR+KTTVS+
Sbjct: 104 AAVDLYHGFVPWCERSDIIKTFPDGSFDAELEIGFKFLVESYVSHVELERAKRIKTTVSN 163
Query: 515 STLFDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRC 336
S+LFDHLINIWEFNPGPVPG+CNLYFLVDFKFQSP+YRQVAS+FFKEVVS+LVGSF++RC
Sbjct: 164 SSLFDHLINIWEFNPGPVPGSCNLYFLVDFKFQSPIYRQVASVFFKEVVSKLVGSFNERC 223
Query: 335 RLVYGPAVPVLENSYGQRA 279
RL+YGP VP+ E++YGQRA
Sbjct: 224 RLIYGPGVPIHEHTYGQRA 242
>gb|KHG15657.1| Coenzyme Q-binding COQ10, mitochondrial [Gossypium arboreum]
Length = 255
Score = 354 bits (909), Expect = 8e-95
Identities = 178/255 (69%), Positives = 207/255 (81%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF+ST+KAV SL+SRR + VR + S+ Q RC S I+G ET S L+
Sbjct: 1 MPPFSSTSKAVGSLISRRIRITHFVRNANNGGCSRSSDQIRCV-STISGTETLSSHPLLA 59
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
S +D S D +Q+RRFLGCGDGEEGG LSKV+EE+ ++GYSPEQLFDVVAAV
Sbjct: 60 SPKEDSFVPSNKISDRYTLQKRRFLGCGDGEEGGALSKVHEEKIIMGYSPEQLFDVVAAV 119
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRSD+++ P+GSFDAELEIGFKFLVESYVS V L RPK +K+TVS+STL
Sbjct: 120 DLYHGFVPWCQRSDIVKQYPNGSFDAELEIGFKFLVESYVSRVVLSRPKFVKSTVSESTL 179
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEFNPGPV GTCNL+FLVDFKFQSPLYRQVASMFFKEVVSRLV SFS+RC+L+
Sbjct: 180 FDHLINIWEFNPGPVLGTCNLHFLVDFKFQSPLYRQVASMFFKEVVSRLVSSFSERCQLI 239
Query: 326 YGPAVPVLENSYGQR 282
YGPA+PVLEN+YG+R
Sbjct: 240 YGPAIPVLENAYGER 254
>ref|XP_003523942.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog B, mitochondrial
isoform X1 [Glycine max] gi|83853823|gb|ABC47856.1|
aromatic-rich family protein [Glycine max]
gi|734322443|gb|KHN04498.1| Coenzyme Q-binding protein
COQ10 like, mitochondrial [Glycine soja]
gi|947112991|gb|KRH61293.1| hypothetical protein
GLYMA_04G039100 [Glycine max]
Length = 251
Score = 354 bits (909), Expect = 8e-95
Identities = 181/256 (70%), Positives = 206/256 (80%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF ST+KA+ SL SR+SG L+R S + S KL RC + I G IG
Sbjct: 1 MPPFLSTSKALCSLASRKSGVSQLIRSS---KSSWKLDGCRCITTAITGHHIQPSFSRIG 57
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
+ L + + + VQ R+FLGCGDGEEG +LS+ YEERRVLGYSPEQLFDVV+AV
Sbjct: 58 FSPL-TGGLCNSNTNYNVVQTRQFLGCGDGEEG-ILSRNYEERRVLGYSPEQLFDVVSAV 115
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
D YHGFVPWCQRS++LRH PDGSFDAELEIGFKFLVESYVSHVELDRPKR+KTTVS STL
Sbjct: 116 DFYHGFVPWCQRSEILRHYPDGSFDAELEIGFKFLVESYVSHVELDRPKRIKTTVSQSTL 175
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
F+HLINIWEFNPGPVPG+C+LYFLVDFKFQSPLYRQ+ASMFFKEV SR+VGSF++RCRLV
Sbjct: 176 FEHLINIWEFNPGPVPGSCDLYFLVDFKFQSPLYRQIASMFFKEVASRMVGSFTERCRLV 235
Query: 326 YGPAVPVLENSYGQRA 279
YGP V VLENSYG+RA
Sbjct: 236 YGPEVRVLENSYGKRA 251
>ref|XP_010059931.1| PREDICTED: coenzyme Q-binding protein COQ10 homolog, mitochondrial
[Eucalyptus grandis] gi|629100945|gb|KCW66414.1|
hypothetical protein EUGRSUZ_F00224 [Eucalyptus grandis]
Length = 250
Score = 353 bits (905), Expect = 2e-94
Identities = 180/256 (70%), Positives = 207/256 (80%)
Frame = -2
Query: 1046 MPPFTSTTKAVLSLLSRRSGFRNLVRYSGKVERSKKLHQSRCFFSGIAGIETGSVRQLIG 867
MPPF ST++AV S LSRR+ + LVR R + RC +GIAG++ + R+
Sbjct: 1 MPPFLSTSRAVRSSLSRRNDIKRLVRSGWSDSRQPVFDRIRCL-AGIAGLDASAGRKA-S 58
Query: 866 SANQDVSSLSGAYFDVSNVQRRRFLGCGDGEEGGVLSKVYEERRVLGYSPEQLFDVVAAV 687
A +DV SG +QRRRFLGCGDGEEGGVL+KVYEE+RVLGYSPEQLFDVVAAV
Sbjct: 59 DAPKDVLVGSGQPI----MQRRRFLGCGDGEEGGVLAKVYEEKRVLGYSPEQLFDVVAAV 114
Query: 686 DLYHGFVPWCQRSDVLRHNPDGSFDAELEIGFKFLVESYVSHVELDRPKRMKTTVSDSTL 507
DLYHGFVPWCQRS+++R+NPDGSFDAELEIGFKFLVESYVSHVEL RP+ +KTT SDS+L
Sbjct: 115 DLYHGFVPWCQRSEIVRNNPDGSFDAELEIGFKFLVESYVSHVELTRPRYIKTTASDSSL 174
Query: 506 FDHLINIWEFNPGPVPGTCNLYFLVDFKFQSPLYRQVASMFFKEVVSRLVGSFSDRCRLV 327
FDHLINIWEFNPGPVPG+C+LYFLVDFKFQSPLYRQVAS+FFKEVV RLV SF++RCRL+
Sbjct: 175 FDHLINIWEFNPGPVPGSCSLYFLVDFKFQSPLYRQVASVFFKEVVGRLVSSFNERCRLI 234
Query: 326 YGPAVPVLENSYGQRA 279
YGP V V E S QRA
Sbjct: 235 YGPGVRVNEESCEQRA 250