BLASTX nr result
ID: Ziziphus21_contig00005033
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ziziphus21_contig00005033
(302 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|KRH59871.1| hypothetical protein GLYMA_05G207600 [Glycine max] 159 1e-36
ref|XP_007210716.1| hypothetical protein PRUPE_ppa022884mg, part... 159 1e-36
ref|XP_003525397.1| PREDICTED: glutamate--cysteine ligase, chlor... 159 1e-36
ref|XP_010540840.1| PREDICTED: glutamate--cysteine ligase, chlor... 158 2e-36
ref|XP_010540831.1| PREDICTED: glutamate--cysteine ligase, chlor... 158 2e-36
gb|AAF22136.1|AF128454_1 gamma-glutamylcysteine synthetase precu... 157 4e-36
ref|XP_007160058.1| hypothetical protein PHAVU_002G289200g [Phas... 157 4e-36
ref|XP_011654747.1| PREDICTED: glutamate--cysteine ligase, chlor... 156 7e-36
gb|KGN50100.1| hypothetical protein Csa_5G153150 [Cucumis sativus] 156 7e-36
ref|XP_008437270.1| PREDICTED: glutamate--cysteine ligase, chlor... 156 7e-36
ref|NP_001292626.1| glutamate--cysteine ligase, chloroplastic [C... 156 7e-36
gb|KRH41171.1| hypothetical protein GLYMA_08G0143001, partial [G... 155 9e-36
gb|KHN16824.1| Glutamate--cysteine ligase, chloroplastic [Glycin... 155 9e-36
ref|XP_010529570.1| PREDICTED: glutamate--cysteine ligase, chlor... 155 9e-36
ref|XP_008238836.1| PREDICTED: glutamate--cysteine ligase, chlor... 155 9e-36
ref|XP_006586325.1| PREDICTED: glutamate--cysteine ligase, chlor... 155 9e-36
ref|XP_007209899.1| hypothetical protein PRUPE_ppa004151mg [Prun... 155 9e-36
ref|XP_004971557.2| PREDICTED: glutamate--cysteine ligase B, chl... 155 1e-35
ref|XP_012086908.1| PREDICTED: glutamate--cysteine ligase, chlor... 155 1e-35
gb|KDP25439.1| hypothetical protein JCGZ_20595 [Jatropha curcas] 155 1e-35
>gb|KRH59871.1| hypothetical protein GLYMA_05G207600 [Glycine max]
Length = 500
Score = 159 bits (401), Expect = 1e-36
Identities = 72/98 (73%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGFE+YVDYALDVPM FV R+NR+I C GKTFRDF+AG+L
Sbjct: 286 WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKNRYIDCTGKTFRDFLAGRLP 345
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 346 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 383
>ref|XP_007210716.1| hypothetical protein PRUPE_ppa022884mg, partial [Prunus persica]
gi|462406451|gb|EMJ11915.1| hypothetical protein
PRUPE_ppa022884mg, partial [Prunus persica]
Length = 463
Score = 159 bits (401), Expect = 1e-36
Identities = 70/98 (71%), Positives = 80/98 (81%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGM+P F+++FGFE+YV+YALD+PM FV R RH C G TFRDFMAGKL
Sbjct: 245 WTDTDKDRTGMIPFVFDDTFGFEKYVEYALDIPMYFVYRNKRHFDCTGMTFRDFMAGKLR 304
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HLGTIY EVRL+RYLEMRGADGGP
Sbjct: 305 CIPGELPTMNDWEMHLGTIYPEVRLKRYLEMRGADGGP 342
>ref|XP_003525397.1| PREDICTED: glutamate--cysteine ligase, chloroplastic isoform X1
[Glycine max] gi|571457117|ref|XP_006580590.1|
PREDICTED: glutamate--cysteine ligase, chloroplastic
isoform X2 [Glycine max] gi|734382515|gb|KHN23647.1|
Glutamate--cysteine ligase, chloroplastic [Glycine soja]
gi|947111546|gb|KRH59872.1| hypothetical protein
GLYMA_05G207600 [Glycine max]
gi|947111547|gb|KRH59873.1| hypothetical protein
GLYMA_05G207600 [Glycine max]
Length = 504
Score = 159 bits (401), Expect = 1e-36
Identities = 72/98 (73%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGFE+YVDYALDVPM FV R+NR+I C GKTFRDF+AG+L
Sbjct: 286 WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKNRYIDCTGKTFRDFLAGRLP 345
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 346 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 383
>ref|XP_010540840.1| PREDICTED: glutamate--cysteine ligase, chloroplastic isoform X2
[Tarenaya hassleriana]
Length = 508
Score = 158 bits (399), Expect = 2e-36
Identities = 71/98 (72%), Positives = 81/98 (82%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F+NSFGFE+YVDYALDVPM FV R ++I C G TFRDF+AGKL
Sbjct: 306 WTDTDKDRTGMLPFVFDNSFGFEQYVDYALDVPMYFVYRNKKYIDCTGSTFRDFVAGKLP 365
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
R+PGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 366 RLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 403
>ref|XP_010540831.1| PREDICTED: glutamate--cysteine ligase, chloroplastic isoform X1
[Tarenaya hassleriana]
Length = 524
Score = 158 bits (399), Expect = 2e-36
Identities = 71/98 (72%), Positives = 81/98 (82%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F+NSFGFE+YVDYALDVPM FV R ++I C G TFRDF+AGKL
Sbjct: 306 WTDTDKDRTGMLPFVFDNSFGFEQYVDYALDVPMYFVYRNKKYIDCTGSTFRDFVAGKLP 365
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
R+PGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 366 RLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 403
>gb|AAF22136.1|AF128454_1 gamma-glutamylcysteine synthetase precursor [Phaseolus vulgaris]
Length = 508
Score = 157 bits (396), Expect = 4e-36
Identities = 71/98 (72%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGFE+YVDYALDVPM FV R++R+I C GKTFRDF+AG+L
Sbjct: 290 WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKHRYIDCTGKTFRDFLAGRLP 349
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 350 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 387
>ref|XP_007160058.1| hypothetical protein PHAVU_002G289200g [Phaseolus vulgaris]
gi|561033473|gb|ESW32052.1| hypothetical protein
PHAVU_002G289200g [Phaseolus vulgaris]
Length = 508
Score = 157 bits (396), Expect = 4e-36
Identities = 71/98 (72%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGFE+YVDYALDVPM FV R++R+I C GKTFRDF+AG+L
Sbjct: 290 WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKHRYIDCTGKTFRDFLAGRLP 349
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 350 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 387
>ref|XP_011654747.1| PREDICTED: glutamate--cysteine ligase, chloroplastic [Cucumis
sativus]
Length = 525
Score = 156 bits (394), Expect = 7e-36
Identities = 68/98 (69%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD+ RTGMLP F++SFGFE+YVDYALDVPM F+ R+ ++I C G +FRDFMAGKL
Sbjct: 307 WTDTDNDRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLP 366
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
+PGE PT+TDWE HL TI+ EVRL+RYLEMRG+DGGP
Sbjct: 367 PVPGELPTLTDWENHLTTIFPEVRLKRYLEMRGSDGGP 404
>gb|KGN50100.1| hypothetical protein Csa_5G153150 [Cucumis sativus]
Length = 501
Score = 156 bits (394), Expect = 7e-36
Identities = 68/98 (69%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD+ RTGMLP F++SFGFE+YVDYALDVPM F+ R+ ++I C G +FRDFMAGKL
Sbjct: 283 WTDTDNDRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLP 342
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
+PGE PT+TDWE HL TI+ EVRL+RYLEMRG+DGGP
Sbjct: 343 PVPGELPTLTDWENHLTTIFPEVRLKRYLEMRGSDGGP 380
>ref|XP_008437270.1| PREDICTED: glutamate--cysteine ligase, chloroplastic [Cucumis melo]
gi|659073821|ref|XP_008437271.1| PREDICTED:
glutamate--cysteine ligase, chloroplastic [Cucumis melo]
gi|659073823|ref|XP_008437272.1| PREDICTED:
glutamate--cysteine ligase, chloroplastic [Cucumis melo]
Length = 525
Score = 156 bits (394), Expect = 7e-36
Identities = 68/98 (69%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD+ RTGMLP F++SFGFE+YVDYALDVPM F+ R+ ++I C G +FRDFMAGKL
Sbjct: 307 WTDTDNDRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLP 366
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
+PGE PT+TDWE HL TI+ EVRL+RYLEMRG+DGGP
Sbjct: 367 PVPGELPTLTDWENHLTTIFPEVRLKRYLEMRGSDGGP 404
>ref|NP_001292626.1| glutamate--cysteine ligase, chloroplastic [Cucumis sativus]
gi|301507724|gb|ADK77882.1| gamma-glutamylcysteine
synthetase [Cucumis sativus]
Length = 525
Score = 156 bits (394), Expect = 7e-36
Identities = 68/98 (69%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD+ RTGMLP F++SFGFE+YVDYALDVPM F+ R+ ++I C G +FRDFMAGKL
Sbjct: 307 WTDTDNDRTGMLPFVFDDSFGFEKYVDYALDVPMYFIYRKKKYIDCSGMSFRDFMAGKLP 366
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
+PGE PT+TDWE HL TI+ EVRL+RYLEMRG+DGGP
Sbjct: 367 PVPGELPTLTDWENHLTTIFPEVRLKRYLEMRGSDGGP 404
>gb|KRH41171.1| hypothetical protein GLYMA_08G0143001, partial [Glycine max]
Length = 500
Score = 155 bits (393), Expect = 9e-36
Identities = 70/98 (71%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGF++YVDYALDVPM FV R++R+I C GKTFRDF+AG+L
Sbjct: 292 WTDTDKDRTGMLPFVFDDSFGFQQYVDYALDVPMYFVYRKHRYIDCTGKTFRDFLAGRLP 351
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 352 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 389
>gb|KHN16824.1| Glutamate--cysteine ligase, chloroplastic [Glycine soja]
Length = 510
Score = 155 bits (393), Expect = 9e-36
Identities = 70/98 (71%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGF++YVDYALDVPM FV R++R+I C GKTFRDF+AG+L
Sbjct: 292 WTDTDKDRTGMLPFVFDDSFGFQQYVDYALDVPMYFVYRKHRYIDCTGKTFRDFLAGRLP 351
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 352 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 389
>ref|XP_010529570.1| PREDICTED: glutamate--cysteine ligase, chloroplastic-like [Tarenaya
hassleriana]
Length = 524
Score = 155 bits (393), Expect = 9e-36
Identities = 70/98 (71%), Positives = 80/98 (81%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD R+GMLP FE+SFGFE+YVDYALDVPM FV R ++I C G TFRDFM GKL
Sbjct: 306 WTDTDKDRSGMLPFVFEDSFGFEQYVDYALDVPMYFVYRNKKYIDCTGSTFRDFMVGKLP 365
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
R+PGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 366 RLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 403
>ref|XP_008238836.1| PREDICTED: glutamate--cysteine ligase, chloroplastic-like [Prunus
mume] gi|645266926|ref|XP_008238837.1| PREDICTED:
glutamate--cysteine ligase, chloroplastic-like [Prunus
mume] gi|645266928|ref|XP_008238838.1| PREDICTED:
glutamate--cysteine ligase, chloroplastic-like [Prunus
mume] gi|645266930|ref|XP_008238840.1| PREDICTED:
glutamate--cysteine ligase, chloroplastic-like [Prunus
mume]
Length = 526
Score = 155 bits (393), Expect = 9e-36
Identities = 70/98 (71%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD++RTGMLP F++SFGFE+YVDYALDVPM FV R+ ++I C G TFRDF+AGKL
Sbjct: 308 WTDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLS 367
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 368 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 405
>ref|XP_006586325.1| PREDICTED: glutamate--cysteine ligase, chloroplastic-like [Glycine
max]
Length = 507
Score = 155 bits (393), Expect = 9e-36
Identities = 70/98 (71%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGF++YVDYALDVPM FV R++R+I C GKTFRDF+AG+L
Sbjct: 292 WTDTDKDRTGMLPFVFDDSFGFQQYVDYALDVPMYFVYRKHRYIDCTGKTFRDFLAGRLP 351
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 352 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 389
>ref|XP_007209899.1| hypothetical protein PRUPE_ppa004151mg [Prunus persica]
gi|462405634|gb|EMJ11098.1| hypothetical protein
PRUPE_ppa004151mg [Prunus persica]
Length = 526
Score = 155 bits (393), Expect = 9e-36
Identities = 70/98 (71%), Positives = 83/98 (84%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD++RTGMLP F++SFGFE+YVDYALDVPM FV R+ ++I C G TFRDF+AGKL
Sbjct: 308 WTDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLS 367
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 368 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 405
>ref|XP_004971557.2| PREDICTED: glutamate--cysteine ligase B, chloroplastic-like
[Setaria italica] gi|944239417|gb|KQL03725.1|
hypothetical protein SETIT_001772mg [Setaria italica]
Length = 393
Score = 155 bits (392), Expect = 1e-35
Identities = 69/98 (70%), Positives = 81/98 (82%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
+T+TDD RTGMLP F +SFGFERYVDY LDVPM FV R NR + C G +FRDFMAGKL
Sbjct: 175 YTDTDDDRTGMLPFVFNSSFGFERYVDYVLDVPMYFVYRNNRPVDCTGMSFRDFMAGKLP 234
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
++PGE PT+ DWE HLG+IY EVRL+R++EMRGADGGP
Sbjct: 235 QLPGERPTLKDWEIHLGSIYPEVRLKRFIEMRGADGGP 272
>ref|XP_012086908.1| PREDICTED: glutamate--cysteine ligase, chloroplastic [Jatropha
curcas]
Length = 526
Score = 155 bits (392), Expect = 1e-35
Identities = 71/98 (72%), Positives = 81/98 (82%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGFE+YVDYALDVPM FV R+ ++I C G TFRDFMAGKL
Sbjct: 308 WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFMAGKLP 367
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 368 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 405
>gb|KDP25439.1| hypothetical protein JCGZ_20595 [Jatropha curcas]
Length = 471
Score = 155 bits (392), Expect = 1e-35
Identities = 71/98 (72%), Positives = 81/98 (82%)
Frame = -2
Query: 295 WTETDDSRTGMLPIAFENSFGFERYVDYALDVPMIFVRRQNRHIGCGGKTFRDFMAGKLE 116
WT+TD RTGMLP F++SFGFE+YVDYALDVPM FV R+ ++I C G TFRDFMAGKL
Sbjct: 253 WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFMAGKLP 312
Query: 115 RIPGETPTITDWECHLGTIYTEVRLRRYLEMRGADGGP 2
IPGE PT+ DWE HL TI+ EVRL+RYLEMRGADGGP
Sbjct: 313 CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGP 350