BLASTX nr result
ID: Zingiber25_contig00019986
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zingiber25_contig00019986
(624 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001055104.1| Os05g0292800 [Oryza sativa Japonica Group] g... 141 1e-31
ref|XP_004246456.1| PREDICTED: uncharacterized protein LOC101262... 131 1e-28
ref|XP_006341011.1| PREDICTED: high-light-induced protein, chlor... 130 3e-28
ref|XP_006654233.1| PREDICTED: high-light-induced protein, chlor... 130 4e-28
gb|EMJ07362.1| hypothetical protein PRUPE_ppa013648mg [Prunus pe... 129 5e-28
ref|NP_001143644.1| hypothetical protein [Zea mays] gi|195623824... 129 9e-28
gb|ACG27460.1| hypothetical protein [Zea mays] 128 1e-27
ref|XP_002531553.1| OHP, putative [Ricinus communis] gi|22352881... 128 1e-27
gb|ABK25530.1| unknown [Picea sitchensis] gi|148906122|gb|ABR162... 128 1e-27
ref|XP_002270357.1| PREDICTED: uncharacterized protein LOC100263... 127 3e-27
gb|AAP23938.1| one helix protein [Ipomoea nil] 127 3e-27
ref|XP_002461455.1| hypothetical protein SORBIDRAFT_02g002950 [S... 126 4e-27
ref|XP_003576368.1| PREDICTED: uncharacterized protein LOC100839... 124 2e-26
gb|EMT00403.1| hypothetical protein F775_28185 [Aegilops tauschii] 124 3e-26
dbj|BAK06217.1| predicted protein [Hordeum vulgare subsp. vulgar... 123 4e-26
ref|XP_002309074.2| hypothetical protein POPTR_0006s08850g [Popu... 122 6e-26
ref|XP_004984567.1| PREDICTED: high-light-induced protein, chlor... 122 6e-26
ref|NP_001234982.1| uncharacterized protein LOC100306434 [Glycin... 121 1e-25
gb|AAM22751.1| one helix protein [Deschampsia antarctica] 121 1e-25
ref|XP_004966884.1| PREDICTED: uncharacterized protein LOC101766... 121 2e-25
>ref|NP_001055104.1| Os05g0292800 [Oryza sativa Japonica Group]
gi|113578655|dbj|BAF17018.1| Os05g0292800 [Oryza sativa
Japonica Group] gi|125551678|gb|EAY97387.1| hypothetical
protein OsI_19311 [Oryza sativa Indica Group]
gi|215765338|dbj|BAG87035.1| unnamed protein product
[Oryza sativa Japonica Group]
gi|222630981|gb|EEE63113.1| hypothetical protein
OsJ_17921 [Oryza sativa Japonica Group]
Length = 112
Score = 141 bits (356), Expect = 1e-31
Identities = 70/104 (67%), Positives = 82/104 (78%), Gaps = 1/104 (0%)
Frame = -2
Query: 503 SPTFLPHQSFPS-VPRRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTR 327
+P+FL HQS S P R L R +S IRAAKLPAGVE+P+ +P+L+EPFLGFTR
Sbjct: 9 APSFLAHQSILSHKPLRKLGLSLELPRTRSVKIRAAKLPAGVEVPRKQPKLSEPFLGFTR 68
Query: 326 TAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
TAE+WNSR CMIGL+G FI EL+ NKG+LQMIGVEVGKGLDLPL
Sbjct: 69 TAEIWNSRACMIGLIGTFIVELVLNKGILQMIGVEVGKGLDLPL 112
>ref|XP_004246456.1| PREDICTED: uncharacterized protein LOC101262449 [Solanum
lycopersicum]
Length = 112
Score = 131 bits (330), Expect = 1e-28
Identities = 63/112 (56%), Positives = 85/112 (75%), Gaps = 5/112 (4%)
Frame = -2
Query: 515 MATISPTFLPHQSFPSVPRRNSSCLP-----RLNRPKSFSIRAAKLPAGVELPKDEPRLT 351
MA++S +FL P+ + + LP ++ + SF+I+AAK+P GVELPK+ P+L+
Sbjct: 1 MASLSSSFLSTTLSPNYSQNHHLFLPNNPNLKITKRTSFNIQAAKVPPGVELPKEVPKLS 60
Query: 350 EPFLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
+P LGFT TAE+WNSR CMIGL+G FI ELIFNKG+L+MIGVE+GKGLD+PL
Sbjct: 61 KPLLGFTNTAEIWNSRACMIGLIGTFIVELIFNKGILEMIGVEIGKGLDIPL 112
>ref|XP_006341011.1| PREDICTED: high-light-induced protein, chloroplastic-like [Solanum
tuberosum]
Length = 112
Score = 130 bits (327), Expect = 3e-28
Identities = 63/112 (56%), Positives = 85/112 (75%), Gaps = 5/112 (4%)
Frame = -2
Query: 515 MATISPTFLPHQSFPSVPRRNSSCLP-----RLNRPKSFSIRAAKLPAGVELPKDEPRLT 351
MA++S +FL + + + LP ++ + SF+I+AAK+PAGVELPK+ P+L+
Sbjct: 1 MASLSSSFLSTTLSANYSQNHHFFLPNNPNLKITKRTSFNIQAAKVPAGVELPKEVPKLS 60
Query: 350 EPFLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
+P LGFT TAE+WNSR CMIGL+G FI ELIFNKG+L+MIGVE+GKGLD+PL
Sbjct: 61 KPLLGFTNTAEIWNSRACMIGLIGTFIVELIFNKGILEMIGVEIGKGLDIPL 112
>ref|XP_006654233.1| PREDICTED: high-light-induced protein, chloroplastic-like [Oryza
brachyantha]
Length = 108
Score = 130 bits (326), Expect = 4e-28
Identities = 59/81 (72%), Positives = 72/81 (88%)
Frame = -2
Query: 437 RLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTRTAEVWNSRVCMIGLVGIFIAELI 258
+L R +S +RAAKLPAGVE+P+++P+L+EPFLGFT+TAEVWNSR CMIGL+G FI EL
Sbjct: 28 QLPRTRSVRVRAAKLPAGVEVPREQPKLSEPFLGFTKTAEVWNSRACMIGLIGTFIVELA 87
Query: 257 FNKGVLQMIGVEVGKGLDLPL 195
NKG+LQMIGVEVGKGLD+PL
Sbjct: 88 LNKGILQMIGVEVGKGLDIPL 108
>gb|EMJ07362.1| hypothetical protein PRUPE_ppa013648mg [Prunus persica]
Length = 111
Score = 129 bits (325), Expect = 5e-28
Identities = 62/108 (57%), Positives = 77/108 (71%)
Frame = -2
Query: 518 SMATISPTFLPHQSFPSVPRRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFL 339
S IS + LP + + + CL R +F ++AAKLPAGV +PK EP+ PFL
Sbjct: 4 SSTIISSSLLPTRVLTANSQNQQLCLLPSKRSAAFRVQAAKLPAGVVVPKVEPKFAAPFL 63
Query: 338 GFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
GFTRTAE+WNSR CMIGL+G FI ELI NKG+LQ+IGVE+GKGLD+PL
Sbjct: 64 GFTRTAEIWNSRACMIGLIGTFIVELILNKGILQLIGVEIGKGLDIPL 111
>ref|NP_001143644.1| hypothetical protein [Zea mays] gi|195623824|gb|ACG33742.1|
hypothetical protein [Zea mays]
gi|414883524|tpg|DAA59538.1| TPA: hypothetical protein
ZEAMMB73_111021 [Zea mays]
Length = 115
Score = 129 bits (323), Expect = 9e-28
Identities = 61/91 (67%), Positives = 75/91 (82%), Gaps = 2/91 (2%)
Frame = -2
Query: 461 RRNSSCLPRLNRPKSFSIRA--AKLPAGVELPKDEPRLTEPFLGFTRTAEVWNSRVCMIG 288
+R + CLP RP + ++RA AKLP GVE P+ +P+L+EPFLGFT+TAE+WNSR CMIG
Sbjct: 25 KRLAPCLPSAPRPGAAALRARAAKLPPGVEAPRVQPKLSEPFLGFTQTAEIWNSRACMIG 84
Query: 287 LVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
L+G FI EL+ NKG+LQMIGVEVGKGLDLPL
Sbjct: 85 LIGTFIVELVLNKGILQMIGVEVGKGLDLPL 115
>gb|ACG27460.1| hypothetical protein [Zea mays]
Length = 116
Score = 128 bits (322), Expect = 1e-27
Identities = 61/91 (67%), Positives = 75/91 (82%), Gaps = 2/91 (2%)
Frame = -2
Query: 461 RRNSSCLPRLNRPKSFSIRA--AKLPAGVELPKDEPRLTEPFLGFTRTAEVWNSRVCMIG 288
+R + CLP RP + ++RA AKLP GVE P+ +P+L+EPFLGFT+TAE+WNSR CMIG
Sbjct: 26 KRLAPCLPSSPRPGAAALRARAAKLPPGVEAPRVQPKLSEPFLGFTQTAEIWNSRACMIG 85
Query: 287 LVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
L+G FI EL+ NKG+LQMIGVEVGKGLDLPL
Sbjct: 86 LIGTFIVELVLNKGILQMIGVEVGKGLDLPL 116
>ref|XP_002531553.1| OHP, putative [Ricinus communis] gi|223528814|gb|EEF30819.1| OHP,
putative [Ricinus communis]
Length = 115
Score = 128 bits (321), Expect = 1e-27
Identities = 65/115 (56%), Positives = 84/115 (73%), Gaps = 8/115 (6%)
Frame = -2
Query: 515 MATISPTFLPHQSFPSVPR-------RNSSCLPRL-NRPKSFSIRAAKLPAGVELPKDEP 360
MA++S + L +S P+ +NS+ + + +P S ++RAAKLP GVELPK +P
Sbjct: 1 MASVSSSLLATKSLTIQPQNQQLLLFKNSAQIRIIPKKPSSLTVRAAKLPPGVELPKVQP 60
Query: 359 RLTEPFLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
+ PFLGFTRTAE+WNSR CMIGL+G FI ELI NKG+LQ+IGV+VGKGLDLPL
Sbjct: 61 KFVAPFLGFTRTAEIWNSRACMIGLIGTFIVELILNKGILQVIGVDVGKGLDLPL 115
>gb|ABK25530.1| unknown [Picea sitchensis] gi|148906122|gb|ABR16219.1| unknown
[Picea sitchensis]
Length = 117
Score = 128 bits (321), Expect = 1e-27
Identities = 63/104 (60%), Positives = 76/104 (73%), Gaps = 5/104 (4%)
Frame = -2
Query: 491 LPHQSFPSVP-----RRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTR 327
LP SF P + N L R+ SF++RAAKLP GVE P++EP+L PFLGFTR
Sbjct: 14 LPSSSFLRSPLCRICKGNEVRLNRIATRPSFTVRAAKLPTGVEAPREEPKLPSPFLGFTR 73
Query: 326 TAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
TAE+WNSR CMIGL+G FI EL+ KG+LQ+IGVE+GKGLDLPL
Sbjct: 74 TAEIWNSRACMIGLIGTFIVELLIRKGILQVIGVEIGKGLDLPL 117
>ref|XP_002270357.1| PREDICTED: uncharacterized protein LOC100263187 [Vitis vinifera]
gi|297740337|emb|CBI30519.3| unnamed protein product
[Vitis vinifera]
Length = 121
Score = 127 bits (319), Expect = 3e-27
Identities = 62/101 (61%), Positives = 75/101 (74%)
Frame = -2
Query: 497 TFLPHQSFPSVPRRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTRTAE 318
+ L Q P R S + + + SF ++AAKLP GVELPK EP+ PFLGFTRTAE
Sbjct: 21 SLLNQQLSPFNHRSPCSTISQPKKKVSFRVQAAKLPPGVELPKVEPKFEAPFLGFTRTAE 80
Query: 317 VWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
+WNSR CMIGL+GIFI ELI NKG+LQ+IGV++GKGLDLPL
Sbjct: 81 IWNSRACMIGLIGIFIVELILNKGILQVIGVDIGKGLDLPL 121
>gb|AAP23938.1| one helix protein [Ipomoea nil]
Length = 116
Score = 127 bits (319), Expect = 3e-27
Identities = 66/111 (59%), Positives = 85/111 (76%), Gaps = 3/111 (2%)
Frame = -2
Query: 518 SMATISPTFLP--HQSFPSVPRRNSSCLPRLNRP-KSFSIRAAKLPAGVELPKDEPRLTE 348
S ++S T P +Q F R ++S + ++P SF ++AAKLPAGVELPK++P+L
Sbjct: 8 SFLSLSTTHTPQHNQLFFFTNRIHTSTIS--HKPISSFKLQAAKLPAGVELPKEQPKLEP 65
Query: 347 PFLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
PFLGFTRTAE+WNSR CMIGL+G FI ELI N+G+LQ+IGV+VGKGLDLPL
Sbjct: 66 PFLGFTRTAEIWNSRACMIGLIGTFIVELILNRGILQLIGVDVGKGLDLPL 116
>ref|XP_002461455.1| hypothetical protein SORBIDRAFT_02g002950 [Sorghum bicolor]
gi|241924832|gb|EER97976.1| hypothetical protein
SORBIDRAFT_02g002950 [Sorghum bicolor]
Length = 111
Score = 126 bits (317), Expect = 4e-27
Identities = 64/108 (59%), Positives = 79/108 (73%), Gaps = 2/108 (1%)
Frame = -2
Query: 512 ATISPTFLPHQSFPSVPRRNSSCLP--RLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFL 339
A +P H+ P R + CLP R R + + AAKLP GVE+P+ +P+L+EPFL
Sbjct: 6 ALSAPCLSAHR--PLCRNRLAPCLPSPRPARAVALRVSAAKLPPGVEVPRVQPKLSEPFL 63
Query: 338 GFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
GFT+TAE+WNSR CMIGL+G FI EL+ NKG+LQMIGVEVGKGLDLPL
Sbjct: 64 GFTQTAEIWNSRACMIGLIGTFIVELVLNKGILQMIGVEVGKGLDLPL 111
>ref|XP_003576368.1| PREDICTED: uncharacterized protein LOC100839853 [Brachypodium
distachyon]
Length = 110
Score = 124 bits (311), Expect = 2e-26
Identities = 60/107 (56%), Positives = 77/107 (71%)
Frame = -2
Query: 515 MATISPTFLPHQSFPSVPRRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLG 336
++T FL PS R + + R + +RAAKLPAGV++P+ +P+L+EPFLG
Sbjct: 4 VSTAHQPFLQANQAPSGNRLQKNAVACSPRASTLRVRAAKLPAGVQVPRVQPKLSEPFLG 63
Query: 335 FTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
FT TAEVWNSR CM+GL+G FI ELI NKGVL++IG EVGKGLD+PL
Sbjct: 64 FTNTAEVWNSRACMMGLIGTFIVELILNKGVLEIIGFEVGKGLDIPL 110
>gb|EMT00403.1| hypothetical protein F775_28185 [Aegilops tauschii]
Length = 109
Score = 124 bits (310), Expect = 3e-26
Identities = 57/87 (65%), Positives = 73/87 (83%)
Frame = -2
Query: 455 NSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTRTAEVWNSRVCMIGLVGI 276
++ C PR +F +RAAKLPAGV++P+ +P+L+EPFLGFT+TAEVWNSR CM+GL+G
Sbjct: 26 SARCSPRAT---AFRVRAAKLPAGVQVPRAQPKLSEPFLGFTKTAEVWNSRACMMGLIGT 82
Query: 275 FIAELIFNKGVLQMIGVEVGKGLDLPL 195
FI ELI NKGVL++IG EVGKGLD+PL
Sbjct: 83 FIVELILNKGVLEIIGFEVGKGLDIPL 109
>dbj|BAK06217.1| predicted protein [Hordeum vulgare subsp. vulgare]
gi|326521100|dbj|BAJ96753.1| predicted protein [Hordeum
vulgare subsp. vulgare]
Length = 108
Score = 123 bits (309), Expect = 4e-26
Identities = 59/103 (57%), Positives = 80/103 (77%)
Frame = -2
Query: 503 SPTFLPHQSFPSVPRRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTRT 324
+P+ L Q+F + ++ R R +F +RAAKLPAGV++P+ +P+L+EPFLGFT+T
Sbjct: 9 APSLLAVQTFRG---QKNAAANRSPRAAAFRVRAAKLPAGVQVPRAQPKLSEPFLGFTKT 65
Query: 323 AEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
AEVWNSR CM+GL+G FI ELI +KGVL++IG EVGKGLD+PL
Sbjct: 66 AEVWNSRACMMGLIGTFIVELILHKGVLEIIGFEVGKGLDIPL 108
>ref|XP_002309074.2| hypothetical protein POPTR_0006s08850g [Populus trichocarpa]
gi|550335811|gb|EEE92597.2| hypothetical protein
POPTR_0006s08850g [Populus trichocarpa]
Length = 119
Score = 122 bits (307), Expect = 6e-26
Identities = 55/85 (64%), Positives = 70/85 (82%)
Frame = -2
Query: 449 SCLPRLNRPKSFSIRAAKLPAGVELPKDEPRLTEPFLGFTRTAEVWNSRVCMIGLVGIFI 270
S + + ++ F I+AAKLP GVELPK EP+ PFLGFTRTAE+WNSR CM+GL+G+F+
Sbjct: 35 SQITKTSKEPVFRIQAAKLPPGVELPKVEPKFQAPFLGFTRTAEIWNSRACMMGLIGVFV 94
Query: 269 AELIFNKGVLQMIGVEVGKGLDLPL 195
ELI NKG+LQ+IGV++GKGLDLPL
Sbjct: 95 VELIINKGILQVIGVDIGKGLDLPL 119
>ref|XP_004984567.1| PREDICTED: high-light-induced protein, chloroplastic-like [Setaria
italica]
Length = 112
Score = 122 bits (307), Expect = 6e-26
Identities = 63/110 (57%), Positives = 82/110 (74%), Gaps = 4/110 (3%)
Frame = -2
Query: 512 ATISPTFLPHQSF--PSVPRRNSSCLPRLNRPKSFSIR--AAKLPAGVELPKDEPRLTEP 345
A SP+ L Q + ++ + CLP P++ +R AAKLP GVE+P+ +P+L+EP
Sbjct: 6 ALSSPSCLVRQPLCGNKLGKKLTPCLPS---PRAVRVRVSAAKLPPGVEVPRVQPKLSEP 62
Query: 344 FLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
FLGFT+TAE+WNSR CMIGL+G FI EL+ NKG+LQ+IGVEVGKGLDLPL
Sbjct: 63 FLGFTQTAEIWNSRACMIGLIGTFIVELVLNKGILQIIGVEVGKGLDLPL 112
>ref|NP_001234982.1| uncharacterized protein LOC100306434 [Glycine max]
gi|255628527|gb|ACU14608.1| unknown [Glycine max]
Length = 118
Score = 121 bits (304), Expect = 1e-25
Identities = 66/118 (55%), Positives = 81/118 (68%), Gaps = 11/118 (9%)
Frame = -2
Query: 515 MATISPTFLPHQSFP-SVPRRNSSCLP-------RLNRPK---SFSIRAAKLPAGVELPK 369
MAT S T L P S+P NS LNRPK SF ++AAK P GVE+PK
Sbjct: 1 MATSSTTLLLSSLIPASIPSFNSGNHSVFLLQNRSLNRPKKRVSFVVQAAKPPIGVEIPK 60
Query: 368 DEPRLTEPFLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
+P+ PFLGFT+TAEVWNSR CMIG++G+FI E I NKG+LQ+IGV+VGKGL++PL
Sbjct: 61 VQPQFKPPFLGFTKTAEVWNSRACMIGIIGVFIVEFIINKGILQVIGVDVGKGLNIPL 118
>gb|AAM22751.1| one helix protein [Deschampsia antarctica]
Length = 110
Score = 121 bits (304), Expect = 1e-25
Identities = 63/113 (55%), Positives = 82/113 (72%), Gaps = 6/113 (5%)
Frame = -2
Query: 515 MATIS----PTFLPHQSFPS--VPRRNSSCLPRLNRPKSFSIRAAKLPAGVELPKDEPRL 354
MA IS P+ L Q+ S + S+C R +F +RA KLPAGV++P+ +P+L
Sbjct: 1 MAVISTACPPSLLAVQTLRSHSFQKNGSTCSTRAT---TFRVRAVKLPAGVQVPRAQPKL 57
Query: 353 TEPFLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
+EPFLGFT+TAEVWNSR CM+GL+G FI ELI NKGVL++IG E+GKGLD+PL
Sbjct: 58 SEPFLGFTKTAEVWNSRACMMGLIGTFIVELILNKGVLEIIGFEMGKGLDIPL 110
>ref|XP_004966884.1| PREDICTED: uncharacterized protein LOC101766786 [Setaria italica]
Length = 212
Score = 121 bits (303), Expect = 2e-25
Identities = 62/110 (56%), Positives = 81/110 (73%), Gaps = 4/110 (3%)
Frame = -2
Query: 512 ATISPTFLPHQSF--PSVPRRNSSCLPRLNRPKSFSIR--AAKLPAGVELPKDEPRLTEP 345
A SP+ L Q + ++ + CLP P++ +R AAKLP GVE+P+ +P+L+EP
Sbjct: 106 ALSSPSCLVRQPLCGNKLGKKLTPCLPS---PRAVRVRVSAAKLPPGVEVPRVQPKLSEP 162
Query: 344 FLGFTRTAEVWNSRVCMIGLVGIFIAELIFNKGVLQMIGVEVGKGLDLPL 195
FLGFT+T E+WNSR CMIGL+G FI EL+ NKG+LQ+IGVEVGKGLDLPL
Sbjct: 163 FLGFTQTVEIWNSRACMIGLIGTFIVELVLNKGILQIIGVEVGKGLDLPL 212