BLASTX nr result
ID: Zingiber24_contig00050581
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zingiber24_contig00050581
(328 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006447749.1| hypothetical protein CICLE_v10014068mg [Citr... 185 5e-45
ref|XP_002531702.1| histidine kinase 1, 2, 3 plant, putative [Ri... 185 6e-45
gb|ESW14494.1| hypothetical protein PHAVU_008G285800g [Phaseolus... 184 1e-44
ref|XP_004491583.1| PREDICTED: histidine kinase 2-like isoform X... 184 1e-44
ref|XP_004491582.1| PREDICTED: histidine kinase 2-like isoform X... 184 1e-44
ref|XP_002269977.2| PREDICTED: histidine kinase 2-like [Vitis vi... 183 2e-44
emb|CBI28424.3| unnamed protein product [Vitis vinifera] 183 2e-44
emb|CAN76309.1| hypothetical protein VITISV_028333 [Vitis vinifera] 183 2e-44
ref|XP_006575741.1| PREDICTED: histidine kinase 2-like isoform X... 182 4e-44
ref|XP_006575738.1| PREDICTED: histidine kinase 2-like isoform X... 182 4e-44
ref|XP_003545073.1| PREDICTED: histidine kinase 2-like [Glycine ... 182 4e-44
ref|XP_004162383.1| PREDICTED: histidine kinase 2-like [Cucumis ... 181 7e-44
ref|XP_004140009.1| PREDICTED: histidine kinase 2-like [Cucumis ... 181 7e-44
gb|ACE63260.1| histidine kinase 2 [Betula pendula] 181 9e-44
gb|EXC34905.1| Histidine kinase 2 [Morus notabilis] 179 3e-43
gb|AAM14700.1| cytokinin receptor [Catharanthus roseus] 179 3e-43
ref|XP_006849055.1| hypothetical protein AMTR_s00028p00193460 [A... 179 4e-43
gb|EOX93453.1| Histidine kinase 2 isoform 3 [Theobroma cacao] 178 6e-43
gb|EOX93452.1| CHASE domain containing histidine kinase protein,... 178 6e-43
gb|EOX93451.1| CHASE domain containing histidine kinase protein,... 178 6e-43
>ref|XP_006447749.1| hypothetical protein CICLE_v10014068mg [Citrus clementina]
gi|567910873|ref|XP_006447750.1| hypothetical protein
CICLE_v10014068mg [Citrus clementina]
gi|568830457|ref|XP_006469515.1| PREDICTED: histidine
kinase 2-like [Citrus sinensis]
gi|557550360|gb|ESR60989.1| hypothetical protein
CICLE_v10014068mg [Citrus clementina]
gi|557550361|gb|ESR60990.1| hypothetical protein
CICLE_v10014068mg [Citrus clementina]
Length = 1223
Score = 185 bits (470), Expect = 5e-45
Identities = 91/107 (85%), Positives = 99/107 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLT PF LLKSNHLGVVLTFAVYNT LP +ATPE+RI AT
Sbjct: 430 MMSGKEDRENILRARASGKGVLTPPFKLLKSNHLGVVLTFAVYNTDLPQDATPEQRIEAT 489
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
+GYLGAS+DVPSLV+KLLHQLASKQTIVVN+Y TTN SAPI MYGPD
Sbjct: 490 LGYLGASYDVPSLVEKLLHQLASKQTIVVNVYDTTNASAPINMYGPD 536
>ref|XP_002531702.1| histidine kinase 1, 2, 3 plant, putative [Ricinus communis]
gi|223528678|gb|EEF30693.1| histidine kinase 1, 2, 3
plant, putative [Ricinus communis]
Length = 922
Score = 185 bits (469), Expect = 6e-45
Identities = 93/107 (86%), Positives = 98/107 (91%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYN LP NATPEERI AT
Sbjct: 127 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNADLPLNATPEERIEAT 186
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLV+KLLHQLASKQTIVVN+Y TTN SAPI MYG D
Sbjct: 187 VGYLGASYDVPSLVEKLLHQLASKQTIVVNVYDTTNASAPILMYGTD 233
>gb|ESW14494.1| hypothetical protein PHAVU_008G285800g [Phaseolus vulgaris]
Length = 1222
Score = 184 bits (467), Expect = 1e-44
Identities = 92/107 (85%), Positives = 100/107 (93%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYNT+LP +ATPE+RI AT
Sbjct: 436 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNTNLPLDATPEQRIEAT 495
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLVDKLLHQLASKQTIVVN+Y TT+ SAPI MYG D
Sbjct: 496 VGYLGASYDVPSLVDKLLHQLASKQTIVVNVYDTTDASAPITMYGTD 542
>ref|XP_004491583.1| PREDICTED: histidine kinase 2-like isoform X2 [Cicer arietinum]
Length = 1204
Score = 184 bits (467), Expect = 1e-44
Identities = 92/107 (85%), Positives = 100/107 (93%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVY+++LPPNATPE+RI AT
Sbjct: 410 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYDSNLPPNATPEQRIEAT 469
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLVDKLLHQLASKQTIVVN+Y TTN SA I MYG D
Sbjct: 470 VGYLGASYDVPSLVDKLLHQLASKQTIVVNVYDTTNASAHITMYGID 516
>ref|XP_004491582.1| PREDICTED: histidine kinase 2-like isoform X1 [Cicer arietinum]
Length = 1208
Score = 184 bits (467), Expect = 1e-44
Identities = 92/107 (85%), Positives = 100/107 (93%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVY+++LPPNATPE+RI AT
Sbjct: 414 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYDSNLPPNATPEQRIEAT 473
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLVDKLLHQLASKQTIVVN+Y TTN SA I MYG D
Sbjct: 474 VGYLGASYDVPSLVDKLLHQLASKQTIVVNVYDTTNASAHITMYGID 520
>ref|XP_002269977.2| PREDICTED: histidine kinase 2-like [Vitis vinifera]
Length = 1272
Score = 183 bits (465), Expect = 2e-44
Identities = 91/105 (86%), Positives = 97/105 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYN LPP+ATPE+RI AT
Sbjct: 477 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNADLPPDATPEQRIEAT 536
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYG 317
VGYLGAS+DVPSLV KLLHQLASKQTIVVN+Y TTN SAPI MYG
Sbjct: 537 VGYLGASYDVPSLVKKLLHQLASKQTIVVNVYDTTNASAPINMYG 581
>emb|CBI28424.3| unnamed protein product [Vitis vinifera]
Length = 1203
Score = 183 bits (465), Expect = 2e-44
Identities = 91/105 (86%), Positives = 97/105 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYN LPP+ATPE+RI AT
Sbjct: 450 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNADLPPDATPEQRIEAT 509
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYG 317
VGYLGAS+DVPSLV KLLHQLASKQTIVVN+Y TTN SAPI MYG
Sbjct: 510 VGYLGASYDVPSLVKKLLHQLASKQTIVVNVYDTTNASAPINMYG 554
>emb|CAN76309.1| hypothetical protein VITISV_028333 [Vitis vinifera]
Length = 1400
Score = 183 bits (465), Expect = 2e-44
Identities = 91/105 (86%), Positives = 97/105 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYN LPP+ATPE+RI AT
Sbjct: 516 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNADLPPDATPEQRIEAT 575
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYG 317
VGYLGAS+DVPSLV KLLHQLASKQTIVVN+Y TTN SAPI MYG
Sbjct: 576 VGYLGASYDVPSLVKKLLHQLASKQTIVVNVYDTTNASAPINMYG 620
>ref|XP_006575741.1| PREDICTED: histidine kinase 2-like isoform X4 [Glycine max]
Length = 1213
Score = 182 bits (462), Expect = 4e-44
Identities = 91/107 (85%), Positives = 98/107 (91%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+D ENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYNT+LP +ATPE+R AT
Sbjct: 427 MMSGKEDHENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNTNLPLDATPEQRTEAT 486
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLVDKLLHQLASKQTIVVN+Y TTN SAPI MYG D
Sbjct: 487 VGYLGASYDVPSLVDKLLHQLASKQTIVVNVYDTTNASAPITMYGTD 533
>ref|XP_006575738.1| PREDICTED: histidine kinase 2-like isoform X1 [Glycine max]
gi|571442471|ref|XP_006575739.1| PREDICTED: histidine
kinase 2-like isoform X2 [Glycine max]
gi|571442473|ref|XP_006575740.1| PREDICTED: histidine
kinase 2-like isoform X3 [Glycine max]
Length = 1221
Score = 182 bits (462), Expect = 4e-44
Identities = 91/107 (85%), Positives = 98/107 (91%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+D ENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYNT+LP +ATPE+R AT
Sbjct: 435 MMSGKEDHENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNTNLPLDATPEQRTEAT 494
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLVDKLLHQLASKQTIVVN+Y TTN SAPI MYG D
Sbjct: 495 VGYLGASYDVPSLVDKLLHQLASKQTIVVNVYDTTNASAPITMYGTD 541
>ref|XP_003545073.1| PREDICTED: histidine kinase 2-like [Glycine max]
Length = 1225
Score = 182 bits (462), Expect = 4e-44
Identities = 92/107 (85%), Positives = 99/107 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYNT+LP +AT E+RI AT
Sbjct: 436 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNTNLPLDATLEQRIEAT 495
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLVDKLLHQLASKQTIVVN+Y TTN SAPI MYG D
Sbjct: 496 VGYLGASYDVPSLVDKLLHQLASKQTIVVNVYDTTNASAPITMYGTD 542
>ref|XP_004162383.1| PREDICTED: histidine kinase 2-like [Cucumis sativus]
Length = 1240
Score = 181 bits (460), Expect = 7e-44
Identities = 90/107 (84%), Positives = 99/107 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVY+T LP +ATPE+RI AT
Sbjct: 447 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYSTDLPLDATPEQRIEAT 506
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+D+PSLV+KLLHQLASKQTIVVN+Y TTN SAPI MYG D
Sbjct: 507 VGYLGASYDIPSLVEKLLHQLASKQTIVVNVYDTTNESAPINMYGSD 553
>ref|XP_004140009.1| PREDICTED: histidine kinase 2-like [Cucumis sativus]
Length = 1240
Score = 181 bits (460), Expect = 7e-44
Identities = 90/107 (84%), Positives = 99/107 (92%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVY+T LP +ATPE+RI AT
Sbjct: 447 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYSTDLPLDATPEQRIEAT 506
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+D+PSLV+KLLHQLASKQTIVVN+Y TTN SAPI MYG D
Sbjct: 507 VGYLGASYDIPSLVEKLLHQLASKQTIVVNVYDTTNESAPINMYGSD 553
>gb|ACE63260.1| histidine kinase 2 [Betula pendula]
Length = 1260
Score = 181 bits (459), Expect = 9e-44
Identities = 89/107 (83%), Positives = 98/107 (91%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DR+NILRARA GKGVLTSPF LLKSNHLGVVLTFAVYNT LPP+ATPE+RI AT
Sbjct: 472 MMSGKEDRDNILRARATGKGVLTSPFKLLKSNHLGVVLTFAVYNTDLPPDATPEKRIEAT 531
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLV+KLLHQLASKQ IVVN+Y TT+ S+PI MYG D
Sbjct: 532 VGYLGASYDVPSLVEKLLHQLASKQMIVVNVYDTTDASSPINMYGTD 578
>gb|EXC34905.1| Histidine kinase 2 [Morus notabilis]
Length = 1326
Score = 179 bits (455), Expect = 3e-43
Identities = 88/107 (82%), Positives = 97/107 (90%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA GKGVLTSPF LLKSN+LGVVLTFAVYNT LPP ATPE+RI AT
Sbjct: 498 MMSGKEDRENILRARATGKGVLTSPFKLLKSNYLGVVLTFAVYNTDLPPEATPEQRIEAT 557
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLV+KLLHQLASK+TIVVN+Y T+ SAP+ MYG D
Sbjct: 558 VGYLGASYDVPSLVEKLLHQLASKETIVVNVYDITDASAPVNMYGTD 604
>gb|AAM14700.1| cytokinin receptor [Catharanthus roseus]
Length = 1041
Score = 179 bits (455), Expect = 3e-43
Identities = 90/108 (83%), Positives = 98/108 (90%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA+GKGVLTSPF LLKSNHLGVVLTFAVYNT LPP AT E+RI AT
Sbjct: 248 MMSGKEDRENILRARASGKGVLTSPFKLLKSNHLGVVLTFAVYNTDLPPEATREQRINAT 307
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPDA 326
VGYLGAS+DVPSLV+KLLHQLASKQTI VN+Y TT+ SAPI+MYG A
Sbjct: 308 VGYLGASYDVPSLVEKLLHQLASKQTIAVNVYDTTDKSAPIKMYGDAA 355
>ref|XP_006849055.1| hypothetical protein AMTR_s00028p00193460 [Amborella trichopoda]
gi|548852528|gb|ERN10636.1| hypothetical protein
AMTR_s00028p00193460 [Amborella trichopoda]
Length = 1004
Score = 179 bits (453), Expect = 4e-43
Identities = 90/107 (84%), Positives = 95/107 (88%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRAR + KGVLTSPF LLKSNHLGVVLTFAVYN LP ATPEERI+AT
Sbjct: 189 MMSGKEDRENILRARTSAKGVLTSPFKLLKSNHLGVVLTFAVYNKDLPSTATPEERIQAT 248
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
GYLGASFDVPSLVDKLL QLASKQTIVVN+Y TTN SAPI MYGP+
Sbjct: 249 QGYLGASFDVPSLVDKLLQQLASKQTIVVNVYDTTNASAPINMYGPN 295
>gb|EOX93453.1| Histidine kinase 2 isoform 3 [Theobroma cacao]
Length = 1047
Score = 178 bits (452), Expect = 6e-43
Identities = 89/107 (83%), Positives = 95/107 (88%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA GKGVLTSPF LLKSNHLGVVLTFAVYN LPP+ATP +R AT
Sbjct: 252 MMSGKEDRENILRARATGKGVLTSPFKLLKSNHLGVVLTFAVYNKDLPPSATPRQRTEAT 311
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLV+KLLHQLASKQTIVVN+Y TTN SA I MYG D
Sbjct: 312 VGYLGASYDVPSLVEKLLHQLASKQTIVVNVYDTTNASAAISMYGTD 358
>gb|EOX93452.1| CHASE domain containing histidine kinase protein, putative isoform
2 [Theobroma cacao]
Length = 1271
Score = 178 bits (452), Expect = 6e-43
Identities = 89/107 (83%), Positives = 95/107 (88%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA GKGVLTSPF LLKSNHLGVVLTFAVYN LPP+ATP +R AT
Sbjct: 476 MMSGKEDRENILRARATGKGVLTSPFKLLKSNHLGVVLTFAVYNKDLPPSATPRQRTEAT 535
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLV+KLLHQLASKQTIVVN+Y TTN SA I MYG D
Sbjct: 536 VGYLGASYDVPSLVEKLLHQLASKQTIVVNVYDTTNASAAISMYGTD 582
>gb|EOX93451.1| CHASE domain containing histidine kinase protein, putative isoform
1 [Theobroma cacao]
Length = 1314
Score = 178 bits (452), Expect = 6e-43
Identities = 89/107 (83%), Positives = 95/107 (88%)
Frame = +3
Query: 3 MMSGKQDRENILRARANGKGVLTSPFNLLKSNHLGVVLTFAVYNTSLPPNATPEERIRAT 182
MMSGK+DRENILRARA GKGVLTSPF LLKSNHLGVVLTFAVYN LPP+ATP +R AT
Sbjct: 476 MMSGKEDRENILRARATGKGVLTSPFKLLKSNHLGVVLTFAVYNKDLPPSATPRQRTEAT 535
Query: 183 VGYLGASFDVPSLVDKLLHQLASKQTIVVNLYHTTNVSAPIRMYGPD 323
VGYLGAS+DVPSLV+KLLHQLASKQTIVVN+Y TTN SA I MYG D
Sbjct: 536 VGYLGASYDVPSLVEKLLHQLASKQTIVVNVYDTTNASAAISMYGTD 582