BLASTX nr result
ID: Zingiber24_contig00044867
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zingiber24_contig00044867
(402 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001065610.2| Os11g0120700 [Oryza sativa Japonica Group] g... 113 3e-23
gb|EEC67562.1| hypothetical protein OsI_34903 [Oryza sativa Indi... 113 3e-23
gb|EAZ19468.1| hypothetical protein OsJ_35034 [Oryza sativa Japo... 113 3e-23
gb|EAY82074.1| hypothetical protein OsI_37270 [Oryza sativa Indi... 112 5e-23
ref|XP_004980120.1| PREDICTED: protein LURP-one-related 5-like [... 104 1e-20
ref|XP_002450168.1| hypothetical protein SORBIDRAFT_05g001420 [S... 104 1e-20
ref|XP_003577125.1| PREDICTED: protein LURP-one-related 12-like ... 104 1e-20
ref|XP_004305058.1| PREDICTED: protein LURP-one-related 5-like [... 98 1e-18
ref|XP_004978337.1| PREDICTED: protein LURP-one-related 5-like [... 97 2e-18
gb|EXC35205.1| hypothetical protein L484_022759 [Morus notabilis] 97 3e-18
ref|NP_001066020.1| Os12g0120500 [Oryza sativa Japonica Group] g... 97 3e-18
gb|EAZ19469.1| hypothetical protein OsJ_35035 [Oryza sativa Japo... 97 3e-18
ref|XP_006663168.1| PREDICTED: protein LURP-one-related 15-like ... 96 4e-18
ref|XP_006663761.1| PREDICTED: protein LURP-one-related 5-like [... 96 5e-18
ref|XP_002450166.1| hypothetical protein SORBIDRAFT_05g001400 [S... 96 6e-18
emb|CBI31132.3| unnamed protein product [Vitis vinifera] 95 8e-18
emb|CAQ58635.1| Unknown gene [Vitis vinifera] 95 8e-18
ref|XP_002264699.2| PREDICTED: protein LURP-one-related 5-like [... 95 8e-18
emb|CAN63770.1| hypothetical protein VITISV_030104 [Vitis vinifera] 95 8e-18
gb|EAY82076.1| hypothetical protein OsI_37273 [Oryza sativa Indi... 95 8e-18
>ref|NP_001065610.2| Os11g0120700 [Oryza sativa Japonica Group]
gi|255679734|dbj|BAF27455.2| Os11g0120700 [Oryza sativa
Japonica Group]
Length = 372
Score = 113 bits (282), Expect = 3e-23
Identities = 60/114 (52%), Positives = 78/114 (68%), Gaps = 10/114 (8%)
Frame = +1
Query: 10 SANKTQVFSMRRPSIFQSCDGAEVFMEPAKG-------RSPAPDFQTEGCFSRRDCKILG 168
S ++ QVFSMR+ S QS D AEVFM SP+P F+ +GCFS R CKI G
Sbjct: 116 SVSQQQVFSMRKSSALQSSDEAEVFMSTRTSGDSQLPDASPSPSFRIDGCFSMRSCKIRG 175
Query: 169 ADGCEVARMGRKKA---NKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
++G E AR+ RK A ++ ++LGDDVF+LV++P VDV +VMA VV+MDRICRR
Sbjct: 176 SNGEEAARITRKNAGVMSRPVSLGDDVFTLVVRPGVDVAVVMAMVVVMDRICRR 229
>gb|EEC67562.1| hypothetical protein OsI_34903 [Oryza sativa Indica Group]
gi|222637514|gb|EEE67646.1| hypothetical protein
OsJ_25239 [Oryza sativa Japonica Group]
Length = 266
Score = 113 bits (282), Expect = 3e-23
Identities = 60/114 (52%), Positives = 78/114 (68%), Gaps = 10/114 (8%)
Frame = +1
Query: 10 SANKTQVFSMRRPSIFQSCDGAEVFMEPAKG-------RSPAPDFQTEGCFSRRDCKILG 168
S ++ QVFSMR+ S QS D AEVFM SP+P F+ +GCFS R CKI G
Sbjct: 116 SVSQQQVFSMRKSSALQSSDEAEVFMSTRTSGDSQLPDASPSPSFRIDGCFSMRSCKIRG 175
Query: 169 ADGCEVARMGRKKA---NKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
++G E AR+ RK A ++ ++LGDDVF+LV++P VDV +VMA VV+MDRICRR
Sbjct: 176 SNGEEAARITRKNAGVMSRPVSLGDDVFTLVVRPGVDVAVVMAMVVVMDRICRR 229
>gb|EAZ19468.1| hypothetical protein OsJ_35034 [Oryza sativa Japonica Group]
Length = 265
Score = 113 bits (282), Expect = 3e-23
Identities = 60/113 (53%), Positives = 79/113 (69%), Gaps = 9/113 (7%)
Frame = +1
Query: 10 SANKTQVFSMRRPSIFQSCDGAEVFM------EPAKGRSPAPDFQTEGCFSRRDCKILGA 171
S ++ QVFSMR+ S QS D AEVFM + SP+P F+ +GCFS R CKI G+
Sbjct: 116 SVSQQQVFSMRKSSALQSNDEAEVFMSTRTSGDSQPDASPSPSFRIDGCFSMRSCKIRGS 175
Query: 172 DGCEVARMGRKKA---NKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
+G E AR+ RK A ++ ++LGDDVF+LV++P VDV +VMA VV+MDRICRR
Sbjct: 176 NGEEAARITRKNAGVMSRPVSLGDDVFTLVVRPGVDVAVVMAMVVVMDRICRR 228
>gb|EAY82074.1| hypothetical protein OsI_37270 [Oryza sativa Indica Group]
Length = 265
Score = 112 bits (280), Expect = 5e-23
Identities = 60/113 (53%), Positives = 78/113 (69%), Gaps = 9/113 (7%)
Frame = +1
Query: 10 SANKTQVFSMRRPSIFQSCDGAEVFM------EPAKGRSPAPDFQTEGCFSRRDCKILGA 171
S ++ QVFSMR+ S QS D AEVFM + SP+P F+ +GCFS R CKI G
Sbjct: 116 SVSQQQVFSMRKSSALQSNDEAEVFMSTRTSGDSQPDASPSPSFRIDGCFSMRSCKIRGR 175
Query: 172 DGCEVARMGRKKA---NKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
+G E AR+ RK A ++ ++LGDDVF+LV++P VDV +VMA VV+MDRICRR
Sbjct: 176 NGEEAARITRKNAGVMSRPVSLGDDVFTLVVRPGVDVAVVMAMVVVMDRICRR 228
>ref|XP_004980120.1| PREDICTED: protein LURP-one-related 5-like [Setaria italica]
Length = 235
Score = 104 bits (260), Expect = 1e-20
Identities = 58/105 (55%), Positives = 72/105 (68%), Gaps = 7/105 (6%)
Frame = +1
Query: 28 VFSMRRPSIFQSCDGAEVFMEPAKGRSP----APDFQTEGCFSRRDCKILGADGCEVARM 195
+FSMR+ SI Q+ A+VFM S P F EGCF RR+CKI +DG EVAR+
Sbjct: 122 LFSMRKCSILQNGHEAQVFMSGCSTASDNPGQGPSFLVEGCFRRRNCKIRNSDGEEVARI 181
Query: 196 GRKKA---NKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
RKKA + S TLGDDVFSLV++PNVD ++MAFVV++DRIC R
Sbjct: 182 MRKKAEAASNSWTLGDDVFSLVVEPNVDCTVIMAFVVVLDRICWR 226
>ref|XP_002450168.1| hypothetical protein SORBIDRAFT_05g001420 [Sorghum bicolor]
gi|241936011|gb|EES09156.1| hypothetical protein
SORBIDRAFT_05g001420 [Sorghum bicolor]
Length = 240
Score = 104 bits (260), Expect = 1e-20
Identities = 56/97 (57%), Positives = 69/97 (71%)
Frame = +1
Query: 25 QVFSMRRPSIFQSCDGAEVFMEPAKGRSPAPDFQTEGCFSRRDCKILGADGCEVARMGRK 204
++FSMR+ S+ Q AEV M G S + F EGCF RR CKI DG EVAR+ RK
Sbjct: 138 RLFSMRKCSVLQKGHEAEVSMS---GSSCSTSFWVEGCFRRRSCKIRNGDGEEVARITRK 194
Query: 205 KANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
K+N S+TLGDDVFSLV+QP VD ++MAFVV++DRIC
Sbjct: 195 KSN-SLTLGDDVFSLVVQPGVDCAMIMAFVVVLDRIC 230
>ref|XP_003577125.1| PREDICTED: protein LURP-one-related 12-like [Brachypodium
distachyon]
Length = 256
Score = 104 bits (259), Expect = 1e-20
Identities = 60/130 (46%), Positives = 75/130 (57%), Gaps = 24/130 (18%)
Frame = +1
Query: 4 GFSANKTQVFSMRRPSIFQSCDGAEVFMEPAKGRSPA--------------PDFQTEGCF 141
G A + ++FSMRR S S D AEV+M + SP P F+ EG F
Sbjct: 116 GCPAPEKKLFSMRRCSALHSSDEAEVYMSSSSPSSPESSSSGRRRCRSPPPPSFRIEGSF 175
Query: 142 SRRDCKILGADGCEVARMGRKKAN----------KSITLGDDVFSLVIQPNVDVELVMAF 291
SRR CKI G+DG E AR+ RKKA + ++LGDDVFSLV++P VD VMA
Sbjct: 176 SRRSCKITGSDGQEAARISRKKAGPTSPATVATLRPVSLGDDVFSLVVRPGVDAATVMAM 235
Query: 292 VVIMDRICRR 321
VV++DRICRR
Sbjct: 236 VVVLDRICRR 245
>ref|XP_004305058.1| PREDICTED: protein LURP-one-related 5-like [Fragaria vesca subsp.
vesca]
Length = 232
Score = 97.8 bits (242), Expect = 1e-18
Identities = 50/108 (46%), Positives = 75/108 (69%), Gaps = 6/108 (5%)
Frame = +1
Query: 10 SANKTQVFSMRRPS--IFQSCDG----AEVFMEPAKGRSPAPDFQTEGCFSRRDCKILGA 171
S+ ++VF+M+ +F + AEVFM ++ SP+PDF+ EGCF RR+CKI +
Sbjct: 116 SSKSSRVFTMKNTGSGLFSRSNKTEATAEVFMG-SETTSPSPDFRIEGCFQRRNCKIRSS 174
Query: 172 DGCEVARMGRKKANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
G VA + RK+ + ++ LG+DVFSLV+QP D++L+MAFVV++DRIC
Sbjct: 175 SGQLVANVARKRVSNTVMLGEDVFSLVVQPGFDLDLIMAFVVVLDRIC 222
>ref|XP_004978337.1| PREDICTED: protein LURP-one-related 5-like [Setaria italica]
Length = 243
Score = 97.1 bits (240), Expect = 2e-18
Identities = 55/111 (49%), Positives = 74/111 (66%), Gaps = 7/111 (6%)
Frame = +1
Query: 10 SANKTQVFSMRRPSIFQSCDGAEVFME----PAKGRSPAPDFQTEGCFSRRDCKILGADG 177
S+ + +FSMR+ S+ QS D AEV + + S AP F+ G F RR CKI +G
Sbjct: 124 SSRRQHLFSMRKCSLVQSNDEAEVHVSGCTTSSDHDSQAPSFRVHGSFWRRSCKIRKGNG 183
Query: 178 CEVARMGRKKA---NKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
EVAR+ RKKA ++++TL +DVFSL I PNVD ++MAFVVI+DRIC+R
Sbjct: 184 EEVARITRKKAGALSETVTLSEDVFSLTIMPNVDRTMIMAFVVILDRICQR 234
>gb|EXC35205.1| hypothetical protein L484_022759 [Morus notabilis]
Length = 441
Score = 96.7 bits (239), Expect = 3e-18
Identities = 51/113 (45%), Positives = 70/113 (61%), Gaps = 11/113 (9%)
Frame = +1
Query: 10 SANKTQVFSMRRPSIFQSCDG------AEVFMEPAKGRSPA-----PDFQTEGCFSRRDC 156
S ++FSM + F S + A+VF+E G++ PDF+ EGCF RR+C
Sbjct: 135 SPKPNKIFSMIKSKSFFSHNSDKNIAEAQVFLERRSGQNKVIIDQTPDFRIEGCFWRRNC 194
Query: 157 KILGADGCEVARMGRKKANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
KI +G VA + RKK N + LGDDVFSLV+QP D +L+MAFV+++DRIC
Sbjct: 195 KIKNRNGEVVANIARKKVNDRVLLGDDVFSLVVQPGFDAQLIMAFVIVLDRIC 247
>ref|NP_001066020.1| Os12g0120500 [Oryza sativa Japonica Group]
gi|77552879|gb|ABA95675.1| expressed protein [Oryza
sativa Japonica Group] gi|113648527|dbj|BAF29039.1|
Os12g0120500 [Oryza sativa Japonica Group]
gi|215737126|dbj|BAG96055.1| unnamed protein product
[Oryza sativa Japonica Group]
Length = 228
Score = 96.7 bits (239), Expect = 3e-18
Identities = 55/112 (49%), Positives = 68/112 (60%), Gaps = 8/112 (7%)
Frame = +1
Query: 4 GFSANKTQVFSMRRPSIFQSCDGAEVFMEPAKGRSP-----APDFQTEGCFSRRDCKILG 168
G A Q+FSMR+ S+ QS AEV M S P F EG F RR CKI
Sbjct: 106 GKRARSQQLFSMRKCSVMQSSHEAEVHMSGCTHASSDRTGHVPAFSIEGSFKRRSCKIRN 165
Query: 169 ADGCEVARMGRKKANK---SITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
+ G EVAR+ RKKA S+TL +DVFSL +QPNVD ++MAFV+++DRIC
Sbjct: 166 SGGEEVARITRKKAGAASLSLTLAEDVFSLEVQPNVDCAMIMAFVIVLDRIC 217
>gb|EAZ19469.1| hypothetical protein OsJ_35035 [Oryza sativa Japonica Group]
Length = 193
Score = 96.7 bits (239), Expect = 3e-18
Identities = 55/112 (49%), Positives = 68/112 (60%), Gaps = 8/112 (7%)
Frame = +1
Query: 4 GFSANKTQVFSMRRPSIFQSCDGAEVFMEPAKGRSP-----APDFQTEGCFSRRDCKILG 168
G A Q+FSMR+ S+ QS AEV M S P F EG F RR CKI
Sbjct: 71 GKRARSQQLFSMRKCSVMQSSHEAEVHMSGCTHASSDRTGHVPAFSIEGSFKRRSCKIRN 130
Query: 169 ADGCEVARMGRKKANK---SITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
+ G EVAR+ RKKA S+TL +DVFSL +QPNVD ++MAFV+++DRIC
Sbjct: 131 SGGEEVARITRKKAGAASLSLTLAEDVFSLEVQPNVDCAMIMAFVIVLDRIC 182
>ref|XP_006663168.1| PREDICTED: protein LURP-one-related 15-like [Oryza brachyantha]
Length = 182
Score = 96.3 bits (238), Expect = 4e-18
Identities = 54/104 (51%), Positives = 71/104 (68%), Gaps = 7/104 (6%)
Frame = +1
Query: 25 QVFSMRRPSIFQSCDGAEVFMEP---AKGRSP-APDFQTEGCFSRRDCKILGADGCEVAR 192
Q+FSMR+ SI Q+ AEV+M A GR+ P F EG F RR CKI ++G +VAR
Sbjct: 68 QLFSMRKCSIMQTSHEAEVYMSGPTNAPGRTGHVPSFCVEGSFRRRSCKIRNSNGEQVAR 127
Query: 193 MGRKKANK---SITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
+ RKKA S+TL +DVFSL +QPNVD ++MAFV+++DRIC
Sbjct: 128 ITRKKAGAAALSLTLAEDVFSLEVQPNVDCAMIMAFVIVLDRIC 171
>ref|XP_006663761.1| PREDICTED: protein LURP-one-related 5-like [Oryza brachyantha]
Length = 232
Score = 95.9 bits (237), Expect = 5e-18
Identities = 54/104 (51%), Positives = 71/104 (68%), Gaps = 7/104 (6%)
Frame = +1
Query: 25 QVFSMRRPSIFQSCDGAEVFMEP---AKGRSP-APDFQTEGCFSRRDCKILGADGCEVAR 192
Q+FSMR+ SI Q+ AEV+M A GR+ P F EG F RR CKI ++G +VAR
Sbjct: 118 QLFSMRKCSIMQTSHEAEVYMSGPTNAPGRTGHVPSFCVEGSFRRRSCKIRNSNGEQVAR 177
Query: 193 MGRKKANK---SITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
+ RKKA S+TL +DVFSL +QPNVD ++MAFV+++DRIC
Sbjct: 178 ITRKKAGGAALSLTLAEDVFSLEVQPNVDCAMIMAFVIVLDRIC 221
>ref|XP_002450166.1| hypothetical protein SORBIDRAFT_05g001400 [Sorghum bicolor]
gi|241936009|gb|EES09154.1| hypothetical protein
SORBIDRAFT_05g001400 [Sorghum bicolor]
Length = 286
Score = 95.5 bits (236), Expect = 6e-18
Identities = 54/111 (48%), Positives = 71/111 (63%), Gaps = 10/111 (9%)
Frame = +1
Query: 19 KTQVFSMRRPSIFQSCDGAEVFMEP-----AKGRSPAPDFQTEGCFSRRDCKIL-GADGC 180
+ +FSMR+ S+ + D AEV+M A G P F +G F RR CKI G DG
Sbjct: 160 RQHLFSMRKCSLVKGTDEAEVYMASGSAAAAHGVQQVPSFTVQGSFWRRSCKIRKGGDGE 219
Query: 181 EVARMGRKK----ANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRICRR 321
EVAR+ RKK A++ +TLG+DVFSL + P+ D +VMAFVV+MDRIC+R
Sbjct: 220 EVARITRKKVGAAASQPVTLGEDVFSLTVMPDADCAMVMAFVVVMDRICQR 270
>emb|CBI31132.3| unnamed protein product [Vitis vinifera]
Length = 187
Score = 95.1 bits (235), Expect = 8e-18
Identities = 53/99 (53%), Positives = 67/99 (67%), Gaps = 3/99 (3%)
Frame = +1
Query: 28 VFSMRRPS--IFQSCDGAEVFMEPAKGRSPAPDFQTEGCFSRRDCKILGA-DGCEVARMG 198
+FSMRR S I + AEVFM AK + PDF+ EG F RR+CKI A G VA++
Sbjct: 79 LFSMRRRSGLIQSKQEEAEVFMGGAKKQRTTPDFRIEGSFRRRNCKIRSAVTGELVAKIA 138
Query: 199 RKKANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
RKK N ++ L DDVFSL +QP D +L+MAFV+I+DRIC
Sbjct: 139 RKKVNTTVLLADDVFSLAVQPGFDSQLIMAFVMILDRIC 177
>emb|CAQ58635.1| Unknown gene [Vitis vinifera]
Length = 189
Score = 95.1 bits (235), Expect = 8e-18
Identities = 53/99 (53%), Positives = 67/99 (67%), Gaps = 3/99 (3%)
Frame = +1
Query: 28 VFSMRRPS--IFQSCDGAEVFMEPAKGRSPAPDFQTEGCFSRRDCKILGA-DGCEVARMG 198
+FSMRR S I + AEVFM AK + PDF+ EG F RR+CKI A G VA++
Sbjct: 81 LFSMRRRSGLIQSKQEEAEVFMGGAKKQRTTPDFRIEGSFRRRNCKIRSAVTGELVAKIA 140
Query: 199 RKKANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
RKK N ++ L DDVFSL +QP D +L+MAFV+I+DRIC
Sbjct: 141 RKKVNTTVLLADDVFSLAVQPGFDSQLIMAFVMILDRIC 179
>ref|XP_002264699.2| PREDICTED: protein LURP-one-related 5-like [Vitis vinifera]
gi|239056177|emb|CAQ58611.1| Unknown gene [Vitis
vinifera]
Length = 240
Score = 95.1 bits (235), Expect = 8e-18
Identities = 53/99 (53%), Positives = 67/99 (67%), Gaps = 3/99 (3%)
Frame = +1
Query: 28 VFSMRRPS--IFQSCDGAEVFMEPAKGRSPAPDFQTEGCFSRRDCKILGA-DGCEVARMG 198
+FSMRR S I + AEVFM AK + PDF+ EG F RR+CKI A G VA++
Sbjct: 132 LFSMRRRSGLIQSKQEEAEVFMGGAKKQRTTPDFRIEGSFRRRNCKIRSAVTGELVAKIA 191
Query: 199 RKKANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
RKK N ++ L DDVFSL +QP D +L+MAFV+I+DRIC
Sbjct: 192 RKKVNTTVLLADDVFSLAVQPGFDSQLIMAFVMILDRIC 230
>emb|CAN63770.1| hypothetical protein VITISV_030104 [Vitis vinifera]
Length = 211
Score = 95.1 bits (235), Expect = 8e-18
Identities = 53/99 (53%), Positives = 67/99 (67%), Gaps = 3/99 (3%)
Frame = +1
Query: 28 VFSMRRPS--IFQSCDGAEVFMEPAKGRSPAPDFQTEGCFSRRDCKILGA-DGCEVARMG 198
+FSMRR S I + AEVFM AK + PDF+ EG F RR+CKI A G VA++
Sbjct: 103 LFSMRRRSGLIQSKQEEAEVFMGGAKKQRTTPDFRIEGSFRRRNCKIRSAVTGELVAKIA 162
Query: 199 RKKANKSITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
RKK N ++ L DDVFSL +QP D +L+MAFV+I+DRIC
Sbjct: 163 RKKVNTTVLLADDVFSLAVQPGFDSQLIMAFVMILDRIC 201
>gb|EAY82076.1| hypothetical protein OsI_37273 [Oryza sativa Indica Group]
Length = 193
Score = 95.1 bits (235), Expect = 8e-18
Identities = 54/112 (48%), Positives = 67/112 (59%), Gaps = 8/112 (7%)
Frame = +1
Query: 4 GFSANKTQVFSMRRPSIFQSCDGAEVFMEPAKGRSP-----APDFQTEGCFSRRDCKILG 168
G Q+FSMR+ S+ QS AEV M S P F EG F RR CKI
Sbjct: 71 GKRTRSQQLFSMRKCSVMQSSHEAEVHMSGCTHASSDRTGHVPAFSIEGSFKRRSCKIRN 130
Query: 169 ADGCEVARMGRKKANK---SITLGDDVFSLVIQPNVDVELVMAFVVIMDRIC 315
+ G EVAR+ RKKA S+TL +DVFSL +QPNVD ++MAFV+++DRIC
Sbjct: 131 SGGEEVARITRKKAGAASLSLTLAEDVFSLEVQPNVDCAMIMAFVIVLDRIC 182