BLASTX nr result
ID: Zingiber23_contig00024934
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Zingiber23_contig00024934 (310 letters) Database: ./nr 37,332,560 sequences; 13,225,080,153 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_003567920.1| PREDICTED: probable ubiquitin-conjugating en... 139 5e-31 ref|XP_004963607.1| PREDICTED: LOW QUALITY PROTEIN: probable ubi... 138 7e-31 gb|EAY98981.1| hypothetical protein OsI_20939 [Oryza sativa Indi... 134 1e-29 ref|NP_001056290.1| Os05g0557600 [Oryza sativa Japonica Group] g... 134 1e-29 gb|EMS55769.1| putative ubiquitin-conjugating enzyme E2 25 [Trit... 133 3e-29 gb|ACR38043.1| unknown [Zea mays] gi|413946463|gb|AFW79112.1| ub... 133 3e-29 gb|EMT05463.1| Putative ubiquitin carrier protein E2 25 [Aegilop... 132 4e-29 ref|XP_002441499.1| hypothetical protein SORBIDRAFT_09g028100 [S... 130 1e-28 ref|NP_001105287.1| LOC542204 [Zea mays] gi|55783585|gb|AAV65291... 130 2e-28 gb|AFW81061.1| hypothetical protein ZEAMMB73_421471 [Zea mays] 128 9e-28 gb|AFW81060.1| hypothetical protein ZEAMMB73_421471 [Zea mays] 128 9e-28 gb|AFW81059.1| hypothetical protein ZEAMMB73_421471 [Zea mays] 128 9e-28 gb|ACR37734.1| unknown [Zea mays] 128 9e-28 gb|EEC84288.1| hypothetical protein OsI_30763 [Oryza sativa Indi... 127 1e-27 dbj|BAK04862.1| predicted protein [Hordeum vulgare subsp. vulgare] 127 2e-27 ref|NP_001062795.1| Os09g0294300 [Oryza sativa Japonica Group] g... 125 4e-27 ref|XP_002456132.1| hypothetical protein SORBIDRAFT_03g031060 [S... 120 2e-25 ref|XP_006661085.1| PREDICTED: probable ubiquitin-conjugating en... 118 7e-25 gb|EEC71265.1| hypothetical protein OsI_03255 [Oryza sativa Indi... 117 2e-24 gb|EAZ13077.1| hypothetical protein OsJ_02997 [Oryza sativa Japo... 117 2e-24 >ref|XP_003567920.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like [Brachypodium distachyon] Length = 509 Score = 139 bits (349), Expect = 5e-31 Identities = 71/101 (70%), Positives = 83/101 (82%), Gaps = 1/101 (0%) Frame = -1 Query: 301 AAEESKRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDW 125 AAE S + K I IV+D+VD Y +FKQFDTV HSDHY+S+PEL + V VKK PSKDW Sbjct: 187 AAEMSNKTKPIMIVDDKVDEKYNAFKQFDTVDCHSDHYYSKPEL-RRVQVVKK--PSKDW 243 Query: 124 TKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KRIQHEWKVLEKDLP+TI+VR YEDR+D+LRAVI+GPAGT Sbjct: 244 AKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVIMGPAGT 284 >ref|XP_004963607.1| PREDICTED: LOW QUALITY PROTEIN: probable ubiquitin-conjugating enzyme E2 24-like [Setaria italica] Length = 1396 Score = 138 bits (348), Expect = 7e-31 Identities = 69/99 (69%), Positives = 83/99 (83%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K K IV+D+++ YK+FKQFDTV +HSDHYFS+PEL K V VKK PSKDW K Sbjct: 1080 ANKTKPTK--IVDDKIEEKYKAFKQFDTVDDHSDHYFSKPELRK-VQVVKK--PSKDWAK 1134 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVLEKDLP+TI+VR YEDR+D+LRAVI+GPAGT Sbjct: 1135 RIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVIMGPAGT 1173 Score = 70.9 bits (172), Expect = 2e-10 Identities = 33/76 (43%), Positives = 49/76 (64%) Frame = -1 Query: 229 KQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTKRIQHEWKVLEKDLPETIYVRVYE 50 K+FD V + SDH+F V N ++ W K++Q EW +L+ DLP+ I+ RV+E Sbjct: 606 KRFDVVTDCSDHHF--------VKESGHENVTRGWVKKVQQEWTILQNDLPDGIHARVFE 657 Query: 49 DRIDILRAVIIGPAGT 2 +R+D+LRA I+G AGT Sbjct: 658 ERMDLLRACIVGAAGT 673 >gb|EAY98981.1| hypothetical protein OsI_20939 [Oryza sativa Indica Group] Length = 509 Score = 134 bits (337), Expect = 1e-29 Identities = 65/101 (64%), Positives = 83/101 (82%), Gaps = 1/101 (0%) Frame = -1 Query: 301 AAEESKRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDW 125 AAE + + K + +++D++D Y +FKQFDTV +HSDHY+S+P+L K V VKK PSK+W Sbjct: 189 AAEMANKTKPVNMMDDKIDEKYSAFKQFDTVDDHSDHYYSKPDLRK-VQVVKK--PSKEW 245 Query: 124 TKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KRIQHEWKVLEKDLP+TI+VR YEDR+D+LRAVI GPAGT Sbjct: 246 AKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVITGPAGT 286 >ref|NP_001056290.1| Os05g0557600 [Oryza sativa Japonica Group] gi|49328017|gb|AAT58718.1| putative ubiquitin-conjugating enzyme [Oryza sativa Japonica Group] gi|113579841|dbj|BAF18204.1| Os05g0557600 [Oryza sativa Japonica Group] gi|222632521|gb|EEE64653.1| hypothetical protein OsJ_19507 [Oryza sativa Japonica Group] Length = 509 Score = 134 bits (337), Expect = 1e-29 Identities = 65/101 (64%), Positives = 83/101 (82%), Gaps = 1/101 (0%) Frame = -1 Query: 301 AAEESKRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDW 125 AAE + + K + +++D++D Y +FKQFDTV +HSDHY+S+P+L K V VKK PSK+W Sbjct: 189 AAEMANKTKPVNMMDDKIDEKYSAFKQFDTVDDHSDHYYSKPDLRK-VQVVKK--PSKEW 245 Query: 124 TKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KRIQHEWKVLEKDLP+TI+VR YEDR+D+LRAVI GPAGT Sbjct: 246 AKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVITGPAGT 286 >gb|EMS55769.1| putative ubiquitin-conjugating enzyme E2 25 [Triticum urartu] Length = 488 Score = 133 bits (334), Expect = 3e-29 Identities = 67/101 (66%), Positives = 82/101 (81%), Gaps = 1/101 (0%) Frame = -1 Query: 301 AAEESKRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDW 125 AAE S +K I I++++VD Y +FKQFDTV H+DHY+ +PEL K + VKK PSKDW Sbjct: 186 AAEMSSNSKPIPILDEKVDERYNTFKQFDTVDGHNDHYYVKPELRKAL-VVKK--PSKDW 242 Query: 124 TKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KRIQHEWKVLEKDLP+TI+VR YEDR+D+LRAVI+GPAGT Sbjct: 243 AKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRAVIMGPAGT 283 >gb|ACR38043.1| unknown [Zea mays] gi|413946463|gb|AFW79112.1| ubiquitin-conjugating enzyme E2 [Zea mays] Length = 507 Score = 133 bits (334), Expect = 3e-29 Identities = 66/99 (66%), Positives = 82/99 (82%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K +K V+D+++ YK+FK+FDTV +HSDHY+S+PE K V VKK PSKDW K Sbjct: 191 ANKTKPSKA--VDDKIEEKYKAFKRFDTVDDHSDHYYSKPESRK-VQAVKK--PSKDWVK 245 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVLEKDLPETI+VR YEDR+D+LRAVI+GPAGT Sbjct: 246 RIQHEWKVLEKDLPETIFVRAYEDRMDLLRAVIMGPAGT 284 >gb|EMT05463.1| Putative ubiquitin carrier protein E2 25 [Aegilops tauschii] Length = 1095 Score = 132 bits (333), Expect = 4e-29 Identities = 67/101 (66%), Positives = 81/101 (80%), Gaps = 1/101 (0%) Frame = -1 Query: 301 AAEESKRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDW 125 AAE S +K I I++++VD Y +FKQFDTV HSDHY+ +PEL K + VKK PSKDW Sbjct: 195 AAEMSSNSKPIPILDEKVDERYSTFKQFDTVDGHSDHYYVKPELRKAL-VVKK--PSKDW 251 Query: 124 TKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KRIQHEWKVLEKDLP+TI+VR YEDR+D+LR VI+GPAGT Sbjct: 252 AKRIQHEWKVLEKDLPDTIFVRAYEDRMDLLRVVIMGPAGT 292 >ref|XP_002441499.1| hypothetical protein SORBIDRAFT_09g028100 [Sorghum bicolor] gi|241946784|gb|EES19929.1| hypothetical protein SORBIDRAFT_09g028100 [Sorghum bicolor] Length = 507 Score = 130 bits (328), Expect = 1e-28 Identities = 61/88 (69%), Positives = 76/88 (86%) Frame = -1 Query: 265 VEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTKRIQHEWKVLEK 86 V+D+++ YK+FK+FDTV +HSDHY+S+PE + V VKK PSKDW KRIQHEWKVLEK Sbjct: 200 VDDKIEEKYKAFKRFDTVDDHSDHYYSKPEK-RRVQVVKK--PSKDWVKRIQHEWKVLEK 256 Query: 85 DLPETIYVRVYEDRIDILRAVIIGPAGT 2 DLP+TI+VR YEDR+D+LRAVI+GPAGT Sbjct: 257 DLPDTIFVRAYEDRMDLLRAVIMGPAGT 284 >ref|NP_001105287.1| LOC542204 [Zea mays] gi|55783585|gb|AAV65291.1| ubiquitin-conjugating enzyme E2 [Zea mays] Length = 505 Score = 130 bits (327), Expect = 2e-28 Identities = 64/99 (64%), Positives = 82/99 (82%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K +K V+D+++ YK+FK+FDTV +HSDHY+S+PE K V VKK PSKDW K Sbjct: 191 ANKTKPSKA--VDDKIEEKYKAFKRFDTVDDHSDHYYSKPESRK-VQAVKK--PSKDWVK 245 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVL+KDLP+TI+VR YEDR+D+LRAVI+GPAGT Sbjct: 246 RIQHEWKVLKKDLPDTIFVRAYEDRMDLLRAVIMGPAGT 284 >gb|AFW81061.1| hypothetical protein ZEAMMB73_421471 [Zea mays] Length = 520 Score = 128 bits (321), Expect = 9e-28 Identities = 62/99 (62%), Positives = 80/99 (80%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K K +V+D+++ YK+FK+FDTV +HSDHY+S+P K V VKK PSKDW K Sbjct: 184 ANKTKPTK--VVDDKIEEKYKAFKRFDTVDDHSDHYYSKPTSRK-VQVVKK--PSKDWVK 238 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVLE+DLP+TI+VR YEDR+D+LR VI+GPAGT Sbjct: 239 RIQHEWKVLERDLPDTIFVRAYEDRMDLLRGVIMGPAGT 277 >gb|AFW81060.1| hypothetical protein ZEAMMB73_421471 [Zea mays] Length = 504 Score = 128 bits (321), Expect = 9e-28 Identities = 62/99 (62%), Positives = 80/99 (80%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K K +V+D+++ YK+FK+FDTV +HSDHY+S+P K V VKK PSKDW K Sbjct: 184 ANKTKPTK--VVDDKIEEKYKAFKRFDTVDDHSDHYYSKPTSRK-VQVVKK--PSKDWVK 238 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVLE+DLP+TI+VR YEDR+D+LR VI+GPAGT Sbjct: 239 RIQHEWKVLERDLPDTIFVRAYEDRMDLLRGVIMGPAGT 277 >gb|AFW81059.1| hypothetical protein ZEAMMB73_421471 [Zea mays] Length = 424 Score = 128 bits (321), Expect = 9e-28 Identities = 62/99 (62%), Positives = 80/99 (80%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K K +V+D+++ YK+FK+FDTV +HSDHY+S+P K V VKK PSKDW K Sbjct: 184 ANKTKPTK--VVDDKIEEKYKAFKRFDTVDDHSDHYYSKPTSRK-VQVVKK--PSKDWVK 238 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVLE+DLP+TI+VR YEDR+D+LR VI+GPAGT Sbjct: 239 RIQHEWKVLERDLPDTIFVRAYEDRMDLLRGVIMGPAGT 277 >gb|ACR37734.1| unknown [Zea mays] Length = 420 Score = 128 bits (321), Expect = 9e-28 Identities = 62/99 (62%), Positives = 80/99 (80%) Frame = -1 Query: 298 AEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTK 119 A ++K K +V+D+++ YK+FK+FDTV +HSDHY+S+P K V VKK PSKDW K Sbjct: 184 ANKTKPTK--VVDDKIEEKYKAFKRFDTVDDHSDHYYSKPTSRK-VQVVKK--PSKDWVK 238 Query: 118 RIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 RIQHEWKVLE+DLP+TI+VR YEDR+D+LR VI+GPAGT Sbjct: 239 RIQHEWKVLERDLPDTIFVRAYEDRMDLLRGVIMGPAGT 277 >gb|EEC84288.1| hypothetical protein OsI_30763 [Oryza sativa Indica Group] Length = 433 Score = 127 bits (320), Expect = 1e-27 Identities = 63/96 (65%), Positives = 80/96 (83%), Gaps = 1/96 (1%) Frame = -1 Query: 286 KRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTKRIQ 110 +++K+I IV++++D YK+FKQFDTV +HSDHY+S VP VKK PSKDW +RIQ Sbjct: 118 RKHKEIKIVDNKIDEKYKAFKQFDTVTDHSDHYYSMSGK-GNVPEVKK--PSKDWVRRIQ 174 Query: 109 HEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 HEWKVLEKDLP TI+VRVYEDR+++LRAVI+GPAGT Sbjct: 175 HEWKVLEKDLPGTIFVRVYEDRMELLRAVIVGPAGT 210 >dbj|BAK04862.1| predicted protein [Hordeum vulgare subsp. vulgare] Length = 530 Score = 127 bits (319), Expect = 2e-27 Identities = 63/101 (62%), Positives = 76/101 (75%) Frame = -1 Query: 304 PAAEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDW 125 PA +R + IVE E+D YK+FKQFDTV +HSDH++ + TVKK PSKDW Sbjct: 218 PADSTQQRKQVKIVESEIDEKYKAFKQFDTVGDHSDHFYLK------FRTVKK--PSKDW 269 Query: 124 TKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KRIQHEWKVL KDLP+TI+VR YEDR+D+LRAVI+GPAGT Sbjct: 270 VKRIQHEWKVLGKDLPDTIFVRAYEDRMDLLRAVIVGPAGT 310 >ref|NP_001062795.1| Os09g0294300 [Oryza sativa Japonica Group] gi|50726452|dbj|BAD34060.1| ubiquitin-conjugating enzyme-like protein [Oryza sativa Japonica Group] gi|113631028|dbj|BAF24709.1| Os09g0294300 [Oryza sativa Japonica Group] gi|222641250|gb|EEE69382.1| hypothetical protein OsJ_28735 [Oryza sativa Japonica Group] Length = 433 Score = 125 bits (315), Expect = 4e-27 Identities = 62/96 (64%), Positives = 79/96 (82%), Gaps = 1/96 (1%) Frame = -1 Query: 286 KRNKKI-IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTKRIQ 110 +++K+I V++++D YK+FKQFDTV +HSDHY+S VP VKK PSKDW +RIQ Sbjct: 118 RKHKEIKTVDNKIDEKYKAFKQFDTVTDHSDHYYSMSGK-GNVPEVKK--PSKDWVRRIQ 174 Query: 109 HEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 HEWKVLEKDLP TI+VRVYEDR+++LRAVI+GPAGT Sbjct: 175 HEWKVLEKDLPGTIFVRVYEDRMELLRAVIVGPAGT 210 >ref|XP_002456132.1| hypothetical protein SORBIDRAFT_03g031060 [Sorghum bicolor] gi|241928107|gb|EES01252.1| hypothetical protein SORBIDRAFT_03g031060 [Sorghum bicolor] Length = 495 Score = 120 bits (301), Expect = 2e-25 Identities = 59/89 (66%), Positives = 72/89 (80%) Frame = -1 Query: 268 IVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSKDWTKRIQHEWKVLE 89 +V E+D YK+FKQFDTV ++SD++++ + V VKK PSK W KRIQHEWKVLE Sbjct: 191 VVNTEIDEKYKTFKQFDTVSDYSDNFYALTGQ-RNVHAVKK--PSKAWVKRIQHEWKVLE 247 Query: 88 KDLPETIYVRVYEDRIDILRAVIIGPAGT 2 KDLPETIYVRVYEDR+D+LRAVI+GPAGT Sbjct: 248 KDLPETIYVRVYEDRMDLLRAVIVGPAGT 276 >ref|XP_006661085.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 26-like [Oryza brachyantha] Length = 407 Score = 118 bits (296), Expect = 7e-25 Identities = 61/103 (59%), Positives = 75/103 (72%) Frame = -1 Query: 310 KNPAAEESKRNKKIIVEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKINPSK 131 KN AE+ K V+ + + YK+FKQFDTV +HSDH++S V T PSK Sbjct: 89 KNGLAEDVKT-----VDSKSNEKYKTFKQFDTVTDHSDHFYSMLGEGNDVVT----KPSK 139 Query: 130 DWTKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 DW KRIQHEWKVLEKDLP+TI+VRVYE+R+D+LRAVI+GPAGT Sbjct: 140 DWVKRIQHEWKVLEKDLPDTIFVRVYENRMDLLRAVIVGPAGT 182 >gb|EEC71265.1| hypothetical protein OsI_03255 [Oryza sativa Indica Group] Length = 420 Score = 117 bits (293), Expect = 2e-24 Identities = 58/102 (56%), Positives = 78/102 (76%), Gaps = 2/102 (1%) Frame = -1 Query: 301 AAEESKRNKKII-VEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKIN-PSKD 128 +AE+ ++K++ V+ ++D Y FKQFD V +HSDH++S P K V+ +N P KD Sbjct: 114 SAEKPHQHKQMKHVDPKIDEKYVEFKQFDIVGDHSDHFYSNPRERK----VQVVNEPGKD 169 Query: 127 WTKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 W KRIQHEWKVLEKDLP+ I+VRVYEDR+++LRAVIIGP+GT Sbjct: 170 WVKRIQHEWKVLEKDLPDNIFVRVYEDRLELLRAVIIGPSGT 211 >gb|EAZ13077.1| hypothetical protein OsJ_02997 [Oryza sativa Japonica Group] Length = 430 Score = 117 bits (293), Expect = 2e-24 Identities = 58/102 (56%), Positives = 78/102 (76%), Gaps = 2/102 (1%) Frame = -1 Query: 301 AAEESKRNKKII-VEDEVDVNYKSFKQFDTVREHSDHYFSRPELLKTVPTVKKIN-PSKD 128 +AE+ ++K++ V+ ++D Y FKQFD V +HSDH++S P K V+ +N P KD Sbjct: 124 SAEKPHQHKQMKHVDPKIDEKYVEFKQFDIVGDHSDHFYSNPRERK----VQVVNEPGKD 179 Query: 127 WTKRIQHEWKVLEKDLPETIYVRVYEDRIDILRAVIIGPAGT 2 W KRIQHEWKVLEKDLP+ I+VRVYEDR+++LRAVIIGP+GT Sbjct: 180 WVKRIQHEWKVLEKDLPDNIFVRVYEDRLELLRAVIIGPSGT 221