BLASTX nr result
ID: Zanthoxylum22_contig00042213
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zanthoxylum22_contig00042213
(329 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006430961.1| hypothetical protein CICLE_v10010885mg [Citr... 190 3e-46
gb|KDO72304.1| hypothetical protein CISIN_1g047869mg [Citrus sin... 190 4e-46
ref|XP_006482440.1| PREDICTED: auxin transport protein BIG-like ... 190 4e-46
ref|XP_007032893.1| Auxin transport protein (BIG) isoform 2 [The... 154 3e-35
ref|XP_007032892.1| Auxin transport protein (BIG) isoform 1 [The... 154 3e-35
ref|XP_011021093.1| PREDICTED: auxin transport protein BIG isofo... 147 4e-33
ref|XP_011021092.1| PREDICTED: auxin transport protein BIG isofo... 147 4e-33
ref|XP_011021091.1| PREDICTED: auxin transport protein BIG isofo... 147 4e-33
gb|KHG12514.1| Auxin transport BIG -like protein [Gossypium arbo... 145 1e-32
gb|KJB10542.1| hypothetical protein B456_001G206500 [Gossypium r... 145 2e-32
gb|KJB10541.1| hypothetical protein B456_001G206500 [Gossypium r... 145 2e-32
gb|KJB10540.1| hypothetical protein B456_001G206500 [Gossypium r... 145 2e-32
gb|KJB10539.1| hypothetical protein B456_001G206500 [Gossypium r... 145 2e-32
ref|XP_012488248.1| PREDICTED: auxin transport protein BIG [Goss... 145 2e-32
ref|XP_006373413.1| hypothetical protein POPTR_0017s13550g [Popu... 144 2e-32
ref|XP_012088111.1| PREDICTED: auxin transport protein BIG [Jatr... 144 3e-32
ref|XP_010660565.1| PREDICTED: auxin transport protein BIG [Viti... 130 4e-28
emb|CBI16927.3| unnamed protein product [Vitis vinifera] 130 4e-28
emb|CAN75802.1| hypothetical protein VITISV_016976 [Vitis vinifera] 130 4e-28
ref|XP_007214891.1| hypothetical protein PRUPE_ppa000002mg [Prun... 129 9e-28
>ref|XP_006430961.1| hypothetical protein CICLE_v10010885mg [Citrus clementina]
gi|557533018|gb|ESR44201.1| hypothetical protein
CICLE_v10010885mg [Citrus clementina]
Length = 5122
Score = 190 bits (483), Expect = 3e-46
Identities = 98/109 (89%), Positives = 100/109 (91%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVMVIKFDKIFESLHEKC IY NLS G ELADYSELFLMKNMEG L DISSREVSD
Sbjct: 1205 LDSVMVIKFDKIFESLHEKCATIYCNLSAGLELADYSELFLMKNMEGYLTDISSREVSDS 1264
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDV+QQVKELYS QRG
Sbjct: 1265 SILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVAQQVKELYSLQRG 1313
>gb|KDO72304.1| hypothetical protein CISIN_1g047869mg [Citrus sinensis]
Length = 2233
Score = 190 bits (482), Expect = 4e-46
Identities = 98/109 (89%), Positives = 100/109 (91%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVMVIKFDKIFESLHEKC IY NLS G ELADYSELFLMKNMEG L DISSREVSD
Sbjct: 1205 LDSVMVIKFDKIFESLHEKCAAIYCNLSAGLELADYSELFLMKNMEGYLTDISSREVSDS 1264
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDV+QQVKELYS QRG
Sbjct: 1265 SILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVAQQVKELYSLQRG 1313
>ref|XP_006482440.1| PREDICTED: auxin transport protein BIG-like [Citrus sinensis]
Length = 5121
Score = 190 bits (482), Expect = 4e-46
Identities = 98/109 (89%), Positives = 100/109 (91%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVMVIKFDKIFESLHEKC IY NLS G ELADYSELFLMKNMEG L DISSREVSD
Sbjct: 1205 LDSVMVIKFDKIFESLHEKCAAIYCNLSAGLELADYSELFLMKNMEGYLTDISSREVSDS 1264
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDV+QQVKELYS QRG
Sbjct: 1265 SILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVAQQVKELYSLQRG 1313
>ref|XP_007032893.1| Auxin transport protein (BIG) isoform 2 [Theobroma cacao]
gi|508711922|gb|EOY03819.1| Auxin transport protein (BIG)
isoform 2 [Theobroma cacao]
Length = 5136
Score = 154 bits (388), Expect = 3e-35
Identities = 71/109 (65%), Positives = 92/109 (84%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+M +K D+IFESLH KCED NL+ G +L+DY+ELFL+K MEG L+D+ SR++ D
Sbjct: 1235 LDSLMSVKLDRIFESLHGKCEDACLNLNAGLDLSDYTELFLLKRMEGFLRDMHSRDLGDT 1294
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
++LEWV+ KTIDTMD LRKDP KS+IFKFYLGAE++S+Q+KEL+ SQRG
Sbjct: 1295 SVLEWVIMKTIDTMDALRKDPSKSVIFKFYLGAENMSEQLKELHGSQRG 1343
>ref|XP_007032892.1| Auxin transport protein (BIG) isoform 1 [Theobroma cacao]
gi|508711921|gb|EOY03818.1| Auxin transport protein (BIG)
isoform 1 [Theobroma cacao]
Length = 5135
Score = 154 bits (388), Expect = 3e-35
Identities = 71/109 (65%), Positives = 92/109 (84%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+M +K D+IFESLH KCED NL+ G +L+DY+ELFL+K MEG L+D+ SR++ D
Sbjct: 1234 LDSLMSVKLDRIFESLHGKCEDACLNLNAGLDLSDYTELFLLKRMEGFLRDMHSRDLGDT 1293
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
++LEWV+ KTIDTMD LRKDP KS+IFKFYLGAE++S+Q+KEL+ SQRG
Sbjct: 1294 SVLEWVIMKTIDTMDALRKDPSKSVIFKFYLGAENMSEQLKELHGSQRG 1342
>ref|XP_011021093.1| PREDICTED: auxin transport protein BIG isoform X3 [Populus
euphratica]
Length = 5064
Score = 147 bits (370), Expect = 4e-33
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM +KFD+IFESL KCEDI NL GSEL+DY++LFLMK+MEG L++I+ R VSD
Sbjct: 1188 LDSVMTVKFDRIFESLQGKCEDIVGNLGTGSELSDYTDLFLMKHMEGFLREINGRGVSDS 1247
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
NI EW++ K I+T D LRKDP KS+IFKFYLGAED+ + +K+ + QRG
Sbjct: 1248 NIYEWIITKIINTADSLRKDPVKSVIFKFYLGAEDMPEMLKDFWGLQRG 1296
>ref|XP_011021092.1| PREDICTED: auxin transport protein BIG isoform X2 [Populus
euphratica]
Length = 5108
Score = 147 bits (370), Expect = 4e-33
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM +KFD+IFESL KCEDI NL GSEL+DY++LFLMK+MEG L++I+ R VSD
Sbjct: 1187 LDSVMTVKFDRIFESLQGKCEDIVGNLGTGSELSDYTDLFLMKHMEGFLREINGRGVSDS 1246
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
NI EW++ K I+T D LRKDP KS+IFKFYLGAED+ + +K+ + QRG
Sbjct: 1247 NIYEWIITKIINTADSLRKDPVKSVIFKFYLGAEDMPEMLKDFWGLQRG 1295
>ref|XP_011021091.1| PREDICTED: auxin transport protein BIG isoform X1 [Populus
euphratica]
Length = 5109
Score = 147 bits (370), Expect = 4e-33
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM +KFD+IFESL KCEDI NL GSEL+DY++LFLMK+MEG L++I+ R VSD
Sbjct: 1188 LDSVMTVKFDRIFESLQGKCEDIVGNLGTGSELSDYTDLFLMKHMEGFLREINGRGVSDS 1247
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
NI EW++ K I+T D LRKDP KS+IFKFYLGAED+ + +K+ + QRG
Sbjct: 1248 NIYEWIITKIINTADSLRKDPVKSVIFKFYLGAEDMPEMLKDFWGLQRG 1296
>gb|KHG12514.1| Auxin transport BIG -like protein [Gossypium arboreum]
Length = 5101
Score = 145 bits (366), Expect = 1e-32
Identities = 68/109 (62%), Positives = 88/109 (80%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+ +K D+IFE L KCED++ NL+ G EL+DY+ELFL+K MEG L+ I SRE+ D
Sbjct: 1181 LDSLTSVKLDRIFERLRGKCEDVWLNLNAGLELSDYTELFLLKRMEGFLRYIHSREMGDT 1240
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWV+ KTIDTMD LRKDP+KS +FKFYLGA D+S+ +KEL+ S+RG
Sbjct: 1241 SILEWVITKTIDTMDALRKDPKKSTLFKFYLGAGDMSESLKELHGSERG 1289
>gb|KJB10542.1| hypothetical protein B456_001G206500 [Gossypium raimondii]
Length = 4286
Score = 145 bits (365), Expect = 2e-32
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+ +K D+IFE L KCED+ NL+ G EL+DY+ELFL+K MEG L+ I SRE+ D
Sbjct: 1181 LDSLTSVKLDRIFERLRGKCEDVCLNLNAGLELSDYTELFLLKRMEGFLRYIHSREMGDT 1240
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWV+ KTIDTMD LRKDP+KS +FKFYLGA D+S+ +KEL+ SQRG
Sbjct: 1241 SILEWVITKTIDTMDALRKDPKKSTLFKFYLGAGDMSESLKELHGSQRG 1289
>gb|KJB10541.1| hypothetical protein B456_001G206500 [Gossypium raimondii]
Length = 4336
Score = 145 bits (365), Expect = 2e-32
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+ +K D+IFE L KCED+ NL+ G EL+DY+ELFL+K MEG L+ I SRE+ D
Sbjct: 1181 LDSLTSVKLDRIFERLRGKCEDVCLNLNAGLELSDYTELFLLKRMEGFLRYIHSREMGDT 1240
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWV+ KTIDTMD LRKDP+KS +FKFYLGA D+S+ +KEL+ SQRG
Sbjct: 1241 SILEWVITKTIDTMDALRKDPKKSTLFKFYLGAGDMSESLKELHGSQRG 1289
>gb|KJB10540.1| hypothetical protein B456_001G206500 [Gossypium raimondii]
Length = 4829
Score = 145 bits (365), Expect = 2e-32
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+ +K D+IFE L KCED+ NL+ G EL+DY+ELFL+K MEG L+ I SRE+ D
Sbjct: 1181 LDSLTSVKLDRIFERLRGKCEDVCLNLNAGLELSDYTELFLLKRMEGFLRYIHSREMGDT 1240
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWV+ KTIDTMD LRKDP+KS +FKFYLGA D+S+ +KEL+ SQRG
Sbjct: 1241 SILEWVITKTIDTMDALRKDPKKSTLFKFYLGAGDMSESLKELHGSQRG 1289
>gb|KJB10539.1| hypothetical protein B456_001G206500 [Gossypium raimondii]
Length = 4702
Score = 145 bits (365), Expect = 2e-32
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+ +K D+IFE L KCED+ NL+ G EL+DY+ELFL+K MEG L+ I SRE+ D
Sbjct: 1181 LDSLTSVKLDRIFERLRGKCEDVCLNLNAGLELSDYTELFLLKRMEGFLRYIHSREMGDT 1240
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWV+ KTIDTMD LRKDP+KS +FKFYLGA D+S+ +KEL+ SQRG
Sbjct: 1241 SILEWVITKTIDTMDALRKDPKKSTLFKFYLGAGDMSESLKELHGSQRG 1289
>ref|XP_012488248.1| PREDICTED: auxin transport protein BIG [Gossypium raimondii]
gi|763743039|gb|KJB10538.1| hypothetical protein
B456_001G206500 [Gossypium raimondii]
Length = 5090
Score = 145 bits (365), Expect = 2e-32
Identities = 69/109 (63%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDS+ +K D+IFE L KCED+ NL+ G EL+DY+ELFL+K MEG L+ I SRE+ D
Sbjct: 1181 LDSLTSVKLDRIFERLRGKCEDVCLNLNAGLELSDYTELFLLKRMEGFLRYIHSREMGDT 1240
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+ILEWV+ KTIDTMD LRKDP+KS +FKFYLGA D+S+ +KEL+ SQRG
Sbjct: 1241 SILEWVITKTIDTMDALRKDPKKSTLFKFYLGAGDMSESLKELHGSQRG 1289
>ref|XP_006373413.1| hypothetical protein POPTR_0017s13550g [Populus trichocarpa]
gi|550320235|gb|ERP51210.1| hypothetical protein
POPTR_0017s13550g [Populus trichocarpa]
Length = 4981
Score = 144 bits (364), Expect = 2e-32
Identities = 68/109 (62%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM +KFD+IFESL KCEDI RNL GSEL+DY++LFLMK+MEG L++I+ R VSD
Sbjct: 1060 LDSVMTVKFDRIFESLQGKCEDIVRNLGTGSELSDYTDLFLMKHMEGFLREINGRGVSDS 1119
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+I EW++ K I+T D L+KDP KS+IFKFYLGAED+ + +K+ QRG
Sbjct: 1120 SIYEWIITKIINTADSLKKDPIKSVIFKFYLGAEDMPEMLKDFCGLQRG 1168
>ref|XP_012088111.1| PREDICTED: auxin transport protein BIG [Jatropha curcas]
Length = 5103
Score = 144 bits (363), Expect = 3e-32
Identities = 70/109 (64%), Positives = 87/109 (79%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
L+SVM+IKFD+IFES+H +CE I RNLS EL+DY+ELFL+K+MEG L+ I++ E D
Sbjct: 1182 LNSVMIIKFDRIFESIHGECESIVRNLSAEFELSDYTELFLLKHMEGFLRQINASEDRDS 1241
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
++LEWV+ KTIDT D LRKDP KS IFKFYLGAEDVS+ VK+ QRG
Sbjct: 1242 SMLEWVITKTIDTADNLRKDPSKSAIFKFYLGAEDVSEPVKDFCGLQRG 1290
>ref|XP_010660565.1| PREDICTED: auxin transport protein BIG [Vitis vinifera]
Length = 5101
Score = 130 bits (327), Expect = 4e-28
Identities = 66/109 (60%), Positives = 82/109 (75%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM IK D+IFESLH CE IY +LS G E +D+S LF MK MEG L+DI++ EVSD
Sbjct: 1173 LDSVMTIKCDRIFESLHGNCEAIYHSLSAGMEGSDFSYLFQMKQMEGFLRDINAGEVSDG 1232
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+I E +V K ID MD+LRKDP ++IFKFY+ DVS++V+ELY QRG
Sbjct: 1233 SIHECIVTKAIDMMDILRKDPSLAVIFKFYVSMVDVSEKVEELYGLQRG 1281
>emb|CBI16927.3| unnamed protein product [Vitis vinifera]
Length = 4380
Score = 130 bits (327), Expect = 4e-28
Identities = 66/109 (60%), Positives = 82/109 (75%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM IK D+IFESLH CE IY +LS G E +D+S LF MK MEG L+DI++ EVSD
Sbjct: 1151 LDSVMTIKCDRIFESLHGNCEAIYHSLSAGMEGSDFSYLFQMKQMEGFLRDINAGEVSDG 1210
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+I E +V K ID MD+LRKDP ++IFKFY+ DVS++V+ELY QRG
Sbjct: 1211 SIHECIVTKAIDMMDILRKDPSLAVIFKFYVSMVDVSEKVEELYGLQRG 1259
>emb|CAN75802.1| hypothetical protein VITISV_016976 [Vitis vinifera]
Length = 5622
Score = 130 bits (327), Expect = 4e-28
Identities = 66/109 (60%), Positives = 82/109 (75%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LDSVM IK D+IFESLH CE IY +LS G E +D+S LF MK MEG L+DI++ EVSD
Sbjct: 1992 LDSVMTIKCDRIFESLHGNCEAIYHSLSAGMEGSDFSYLFQMKQMEGFLRDINAGEVSDG 2051
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+I E +V K ID MD+LRKDP ++IFKFY+ DVS++V+ELY QRG
Sbjct: 2052 SIHECIVTKAIDMMDILRKDPSLAVIFKFYVSMVDVSEKVEELYGLQRG 2100
>ref|XP_007214891.1| hypothetical protein PRUPE_ppa000002mg [Prunus persica]
gi|462411041|gb|EMJ16090.1| hypothetical protein
PRUPE_ppa000002mg [Prunus persica]
Length = 4979
Score = 129 bits (324), Expect = 9e-28
Identities = 63/109 (57%), Positives = 82/109 (75%)
Frame = -2
Query: 328 LDSVMVIKFDKIFESLHEKCEDIYRNLSDGSELADYSELFLMKNMEGILKDISSREVSDK 149
LD+V+ IKFD+IFES+H KCE IY +LS G +DY+ L L++++EG L+DI++R VSD
Sbjct: 1055 LDTVVTIKFDRIFESIHGKCETIYESLSAGLGGSDYANLILLEHLEGFLRDINARGVSDN 1114
Query: 148 NILEWVVAKTIDTMDVLRKDPQKSLIFKFYLGAEDVSQQVKELYSSQRG 2
+I E ++ K ID MD LRKDP K IFKFYLG EDV +QVK L+ QRG
Sbjct: 1115 SIYECIITKAIDMMDSLRKDPTKVDIFKFYLGVEDVPEQVKALFGVQRG 1163