BLASTX nr result
ID: Zanthoxylum22_contig00031517
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Zanthoxylum22_contig00031517
(372 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006447520.1| hypothetical protein CICLE_v10015921mg [Citr... 142 1e-31
emb|CBI28952.3| unnamed protein product [Vitis vinifera] 108 2e-21
ref|XP_002273999.2| PREDICTED: APO protein 4, mitochondrial [Vit... 108 2e-21
ref|XP_006372950.1| hypothetical protein POPTR_0017s06490g [Popu... 108 2e-21
ref|XP_011037010.1| PREDICTED: APO protein 4, mitochondrial [Pop... 104 3e-20
ref|XP_002527241.1| APO protein 4, mitochondrial precursor, puta... 103 5e-20
ref|XP_009365158.1| PREDICTED: APO protein 4, mitochondrial-like... 100 7e-19
ref|XP_009337730.1| PREDICTED: APO protein 4, mitochondrial-like... 99 2e-18
ref|XP_012491256.1| PREDICTED: APO protein 4, mitochondrial [Gos... 97 4e-18
ref|XP_008379620.1| PREDICTED: LOW QUALITY PROTEIN: APO protein ... 97 4e-18
ref|XP_010052452.1| PREDICTED: APO protein 4, mitochondrial [Euc... 97 4e-18
ref|XP_004151879.1| PREDICTED: APO protein 4, mitochondrial [Cuc... 97 4e-18
ref|XP_008455841.1| PREDICTED: APO protein 4, mitochondrial [Cuc... 97 5e-18
ref|XP_007033060.1| APO protein 4 isoform 1 [Theobroma cacao] gi... 97 6e-18
ref|XP_008237745.1| PREDICTED: APO protein 4, mitochondrial-like... 96 1e-17
emb|CAE04242.3| OSJNBa0089N06.3 [Oryza sativa Japonica Group] 92 1e-16
ref|XP_008776097.1| PREDICTED: APO protein 4, mitochondrial isof... 92 1e-16
ref|XP_008230090.1| PREDICTED: APO protein 4, mitochondrial [Pru... 92 1e-16
emb|CAJ86368.1| OSIGBa0117N13.12 [Oryza sativa Indica Group] gi|... 92 1e-16
ref|NP_001053956.1| Os04g0628000 [Oryza sativa Japonica Group] g... 92 1e-16
>ref|XP_006447520.1| hypothetical protein CICLE_v10015921mg [Citrus clementina]
gi|568830870|ref|XP_006469706.1| PREDICTED: APO protein
4, mitochondrial-like [Citrus sinensis]
gi|557550131|gb|ESR60760.1| hypothetical protein
CICLE_v10015921mg [Citrus clementina]
Length = 326
Score = 142 bits (357), Expect = 1e-31
Identities = 71/81 (87%), Positives = 77/81 (95%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARDVLK 66
MALRRKL +F GELS+GQRFYSSKFDLKKLRPMI+KRIENRAKDYP+RDMVPVAR+VLK
Sbjct: 1 MALRRKLWVDFWGELSNGQRFYSSKFDLKKLRPMIMKRIENRAKDYPIRDMVPVAREVLK 60
Query: 65 ARALLIQGVSTLIKVFPVHAC 3
ARALLI+GVSTLIKVFPV AC
Sbjct: 61 ARALLIRGVSTLIKVFPVQAC 81
>emb|CBI28952.3| unnamed protein product [Vitis vinifera]
Length = 200
Score = 108 bits (270), Expect = 2e-21
Identities = 59/84 (70%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSGQ---RFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARD 75
MALR KL F+ E S RFYS K DLKKLRPMILKRIENRAK+YP+ M+PVA+D
Sbjct: 1 MALRGKLWHCFLDEASGFAMYARFYSVKVDLKKLRPMILKRIENRAKEYPISSMIPVAQD 60
Query: 74 VLKARALLIQGVSTLIKVFPVHAC 3
VLKAR+LLIQGVSTL+ VFPV AC
Sbjct: 61 VLKARSLLIQGVSTLMNVFPVMAC 84
>ref|XP_002273999.2| PREDICTED: APO protein 4, mitochondrial [Vitis vinifera]
Length = 329
Score = 108 bits (270), Expect = 2e-21
Identities = 59/84 (70%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSGQ---RFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARD 75
MALR KL F+ E S RFYS K DLKKLRPMILKRIENRAK+YP+ M+PVA+D
Sbjct: 1 MALRGKLWHCFLDEASGFAMYARFYSVKVDLKKLRPMILKRIENRAKEYPISSMIPVAQD 60
Query: 74 VLKARALLIQGVSTLIKVFPVHAC 3
VLKAR+LLIQGVSTL+ VFPV AC
Sbjct: 61 VLKARSLLIQGVSTLMNVFPVMAC 84
>ref|XP_006372950.1| hypothetical protein POPTR_0017s06490g [Populus trichocarpa]
gi|550319598|gb|ERP50747.1| hypothetical protein
POPTR_0017s06490g [Populus trichocarpa]
Length = 328
Score = 108 bits (269), Expect = 2e-21
Identities = 57/85 (67%), Positives = 67/85 (78%), Gaps = 4/85 (4%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSG----QRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVAR 78
MALR+KL N + E S RFYSS+ D KKLRPMILKRI+NRAKDYP++ MVPVAR
Sbjct: 1 MALRKKLWENIVEEFSKTYFMHSRFYSSRVDFKKLRPMILKRIQNRAKDYPVKGMVPVAR 60
Query: 77 DVLKARALLIQGVSTLIKVFPVHAC 3
+VL+ R LLIQGVSTL++VFPV AC
Sbjct: 61 EVLEKRKLLIQGVSTLMEVFPVLAC 85
>ref|XP_011037010.1| PREDICTED: APO protein 4, mitochondrial [Populus euphratica]
gi|743883370|ref|XP_011037011.1| PREDICTED: APO protein
4, mitochondrial [Populus euphratica]
gi|743883383|ref|XP_011037012.1| PREDICTED: APO protein
4, mitochondrial [Populus euphratica]
gi|743883387|ref|XP_011037013.1| PREDICTED: APO protein
4, mitochondrial [Populus euphratica]
Length = 328
Score = 104 bits (259), Expect = 3e-20
Identities = 55/85 (64%), Positives = 65/85 (76%), Gaps = 4/85 (4%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSG----QRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVAR 78
MA +KL N + E S RFYSS+ D KKLRPMILKRI+NRAKDYP++ MVPVAR
Sbjct: 1 MAFTKKLWENLVEEFSKTYFMHSRFYSSRVDFKKLRPMILKRIQNRAKDYPVKGMVPVAR 60
Query: 77 DVLKARALLIQGVSTLIKVFPVHAC 3
+VL+ R LLIQGVSTL++VFPV AC
Sbjct: 61 EVLEKRKLLIQGVSTLMEVFPVLAC 85
>ref|XP_002527241.1| APO protein 4, mitochondrial precursor, putative [Ricinus communis]
gi|223533417|gb|EEF35167.1| APO protein 4, mitochondrial
precursor, putative [Ricinus communis]
Length = 325
Score = 103 bits (257), Expect = 5e-20
Identities = 56/84 (66%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Frame = -2
Query: 245 MALRRKLCPNFM---GELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARD 75
MA R+K+ +F G + RFYSS+ D KKLRPMILKRIENRAKDYP+RD+VPVA +
Sbjct: 1 MASRKKIWQDFARFYGNHITHFRFYSSQVDWKKLRPMILKRIENRAKDYPVRDLVPVAHE 60
Query: 74 VLKARALLIQGVSTLIKVFPVHAC 3
VL+AR LLIQGVSTLI+V PV AC
Sbjct: 61 VLQARMLLIQGVSTLIQVIPVVAC 84
>ref|XP_009365158.1| PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri] gi|694377756|ref|XP_009365159.1|
PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri] gi|694377759|ref|XP_009365160.1|
PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri]
Length = 333
Score = 99.8 bits (247), Expect = 7e-19
Identities = 52/84 (61%), Positives = 68/84 (80%), Gaps = 3/84 (3%)
Frame = -2
Query: 245 MALRRKLCP--NFMGEL-SSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARD 75
MALR+KLC NF G++ S R+YSSK DL+KL+PMI KRI+NRAKDYP+ M+PVA++
Sbjct: 1 MALRKKLCERSNFNGKIMDSFARYYSSKMDLRKLKPMIQKRIQNRAKDYPVPAMLPVAQE 60
Query: 74 VLKARALLIQGVSTLIKVFPVHAC 3
VL +R L++QGVSTL+K+ PV AC
Sbjct: 61 VLDSRRLVMQGVSTLLKMVPVLAC 84
>ref|XP_009337730.1| PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri] gi|694419552|ref|XP_009337732.1|
PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri] gi|694419555|ref|XP_009337733.1|
PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri] gi|694419557|ref|XP_009337734.1|
PREDICTED: APO protein 4, mitochondrial-like [Pyrus x
bretschneideri]
Length = 333
Score = 98.6 bits (244), Expect = 2e-18
Identities = 52/84 (61%), Positives = 67/84 (79%), Gaps = 3/84 (3%)
Frame = -2
Query: 245 MALRRKLCP--NFMGEL-SSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARD 75
MALR+KLC NF G++ S R+YS K DL+KL+PMI KRI+NRAKDYP+ MVPVA++
Sbjct: 1 MALRKKLCERSNFNGKIMDSFARYYSLKMDLRKLKPMIQKRIQNRAKDYPVPAMVPVAQE 60
Query: 74 VLKARALLIQGVSTLIKVFPVHAC 3
VL +R L++QGVSTL+K+ PV AC
Sbjct: 61 VLDSRRLVMQGVSTLLKMVPVLAC 84
>ref|XP_012491256.1| PREDICTED: APO protein 4, mitochondrial [Gossypium raimondii]
gi|823190792|ref|XP_012491257.1| PREDICTED: APO protein
4, mitochondrial [Gossypium raimondii]
gi|763775889|gb|KJB43012.1| hypothetical protein
B456_007G179700 [Gossypium raimondii]
Length = 331
Score = 97.4 bits (241), Expect = 4e-18
Identities = 48/62 (77%), Positives = 54/62 (87%)
Frame = -2
Query: 188 RFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARDVLKARALLIQGVSTLIKVFPVH 9
R YSSK DLKKLRPMILKRIENRAKDYP+ M+PVA++VLKAR +L QGV TLIK+FPV
Sbjct: 10 RSYSSKIDLKKLRPMILKRIENRAKDYPVPGMIPVAQEVLKAREMLFQGVLTLIKLFPVV 69
Query: 8 AC 3
AC
Sbjct: 70 AC 71
>ref|XP_008379620.1| PREDICTED: LOW QUALITY PROTEIN: APO protein 4, mitochondrial [Malus
domestica]
Length = 343
Score = 97.4 bits (241), Expect = 4e-18
Identities = 51/84 (60%), Positives = 65/84 (77%), Gaps = 3/84 (3%)
Frame = -2
Query: 245 MALRRKLCP--NFMGELSSG-QRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARD 75
MALR+KLC NF G++ R+Y SK DL+KL+PMI KRI+NRAKDYP+ MVPVA +
Sbjct: 1 MALRKKLCERSNFNGKIMDNFARYYRSKMDLRKLKPMIQKRIQNRAKDYPVPAMVPVAXE 60
Query: 74 VLKARALLIQGVSTLIKVFPVHAC 3
VL +R L++QGVSTL+K+ PV AC
Sbjct: 61 VLDSRRLVMQGVSTLLKMVPVLAC 84
>ref|XP_010052452.1| PREDICTED: APO protein 4, mitochondrial [Eucalyptus grandis]
gi|702245137|ref|XP_010052460.1| PREDICTED: APO protein
4, mitochondrial [Eucalyptus grandis]
gi|702245142|ref|XP_010052467.1| PREDICTED: APO protein
4, mitochondrial [Eucalyptus grandis]
gi|629125180|gb|KCW89605.1| hypothetical protein
EUGRSUZ_A01889 [Eucalyptus grandis]
Length = 328
Score = 97.4 bits (241), Expect = 4e-18
Identities = 54/87 (62%), Positives = 64/87 (73%), Gaps = 6/87 (6%)
Frame = -2
Query: 245 MALRRKLCP------NFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPV 84
M RR+LC +F G + R YS++ DLKKLRPMILKRIE RAKDYP+ MVPV
Sbjct: 1 MVPRRELCRILLEGRHFCGAYA---RLYSTEVDLKKLRPMILKRIEGRAKDYPVPGMVPV 57
Query: 83 ARDVLKARALLIQGVSTLIKVFPVHAC 3
A++VLKAR LLIQGVSTL++ FPV AC
Sbjct: 58 AQEVLKARTLLIQGVSTLLRFFPVAAC 84
>ref|XP_004151879.1| PREDICTED: APO protein 4, mitochondrial [Cucumis sativus]
gi|700208150|gb|KGN63269.1| hypothetical protein
Csa_2G421010 [Cucumis sativus]
Length = 330
Score = 97.4 bits (241), Expect = 4e-18
Identities = 51/81 (62%), Positives = 64/81 (79%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARDVLK 66
MA+RRK N + E QR YSSK +LKKLRPMILKRI++RAK+YP++ M PVA+ VL+
Sbjct: 7 MAIRRKFRDNVVQEFML-QRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLE 65
Query: 65 ARALLIQGVSTLIKVFPVHAC 3
ARA+LI GVSTL+K FPV +C
Sbjct: 66 ARAMLIHGVSTLLKSFPVLSC 86
>ref|XP_008455841.1| PREDICTED: APO protein 4, mitochondrial [Cucumis melo]
gi|659111658|ref|XP_008455842.1| PREDICTED: APO protein
4, mitochondrial [Cucumis melo]
Length = 334
Score = 97.1 bits (240), Expect = 5e-18
Identities = 51/81 (62%), Positives = 63/81 (77%)
Frame = -2
Query: 245 MALRRKLCPNFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARDVLK 66
MA+RRK N + E QR YSSK +LKKLRPMILKRI++RAK YP++ M PVA+ VL+
Sbjct: 7 MAIRRKFRDNVVQEFML-QRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVAQQVLE 65
Query: 65 ARALLIQGVSTLIKVFPVHAC 3
ARA+LI GVSTL+K FPV +C
Sbjct: 66 ARAMLIHGVSTLLKSFPVLSC 86
>ref|XP_007033060.1| APO protein 4 isoform 1 [Theobroma cacao]
gi|590652090|ref|XP_007033061.1| APO protein 4 isoform 1
[Theobroma cacao] gi|590652093|ref|XP_007033062.1| APO
protein 4 isoform 1 [Theobroma cacao]
gi|508712089|gb|EOY03986.1| APO protein 4 isoform 1
[Theobroma cacao] gi|508712090|gb|EOY03987.1| APO
protein 4 isoform 1 [Theobroma cacao]
gi|508712091|gb|EOY03988.1| APO protein 4 isoform 1
[Theobroma cacao]
Length = 312
Score = 96.7 bits (239), Expect = 6e-18
Identities = 48/62 (77%), Positives = 54/62 (87%)
Frame = -2
Query: 188 RFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVARDVLKARALLIQGVSTLIKVFPVH 9
R YSSK DLKKLRPMILKRIENRAKDYP+ M+PVA++VL ARALL QGVS L+K+FPV
Sbjct: 10 RSYSSKVDLKKLRPMILKRIENRAKDYPVPGMIPVAQEVLMARALLFQGVSILLKLFPVL 69
Query: 8 AC 3
AC
Sbjct: 70 AC 71
>ref|XP_008237745.1| PREDICTED: APO protein 4, mitochondrial-like [Prunus mume]
Length = 327
Score = 95.5 bits (236), Expect = 1e-17
Identities = 49/85 (57%), Positives = 67/85 (78%), Gaps = 4/85 (4%)
Frame = -2
Query: 245 MALRRKLCP--NFMGEL--SSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVAR 78
MALR+KLC NF G+ + + F S+K DL+KL+PMI KRI++RAKDYP++ M+PVA+
Sbjct: 1 MALRKKLCESSNFNGKFMYTFSRYFSSNKMDLRKLKPMIQKRIQDRAKDYPVQGMIPVAQ 60
Query: 77 DVLKARALLIQGVSTLIKVFPVHAC 3
+VL +R L++QGVSTL+KV PV AC
Sbjct: 61 EVLNSRRLIMQGVSTLLKVLPVMAC 85
>emb|CAE04242.3| OSJNBa0089N06.3 [Oryza sativa Japonica Group]
Length = 580
Score = 92.4 bits (228), Expect = 1e-16
Identities = 45/86 (52%), Positives = 65/86 (75%), Gaps = 5/86 (5%)
Frame = -2
Query: 245 MALRRK-----LCPNFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVA 81
MAL R+ +C G + + QR YSS+ D K+LRPMILKRI+NRAK+YP++ M+PVA
Sbjct: 1 MALMRRRVGGYICSELCGSVMN-QRLYSSRVDWKQLRPMILKRIKNRAKEYPIKRMIPVA 59
Query: 80 RDVLKARALLIQGVSTLIKVFPVHAC 3
+V+KAR ++ +GVSTL++V P+H+C
Sbjct: 60 EEVVKAREIVTKGVSTLLQVVPIHSC 85
>ref|XP_008776097.1| PREDICTED: APO protein 4, mitochondrial isoform X1 [Phoenix
dactylifera] gi|672193681|ref|XP_008776098.1| PREDICTED:
APO protein 4, mitochondrial isoform X1 [Phoenix
dactylifera]
Length = 336
Score = 92.4 bits (228), Expect = 1e-16
Identities = 48/85 (56%), Positives = 62/85 (72%), Gaps = 3/85 (3%)
Frame = -2
Query: 248 LMALRRKLCPNFMGELSSG---QRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVAR 78
LMA R K+ + +LS R+YSSK D KKLRPMILKRI+NRAKDYP++ M+PVA
Sbjct: 12 LMAFRLKVQHSIWVDLSCSPMHSRYYSSKVDWKKLRPMILKRIQNRAKDYPVKSMIPVAN 71
Query: 77 DVLKARALLIQGVSTLIKVFPVHAC 3
+VLKAR LL +GVS L++V + +C
Sbjct: 72 EVLKARELLTKGVSALLQVIAIKSC 96
>ref|XP_008230090.1| PREDICTED: APO protein 4, mitochondrial [Prunus mume]
gi|645247983|ref|XP_008230091.1| PREDICTED: APO protein
4, mitochondrial [Prunus mume]
Length = 330
Score = 92.4 bits (228), Expect = 1e-16
Identities = 49/86 (56%), Positives = 66/86 (76%), Gaps = 4/86 (4%)
Frame = -2
Query: 248 LMALRRKL--CPNFMGEL--SSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVA 81
+MALR+KL NF G++ + + F S+K DL+KL+PMI KRI+ RAKDYP+ M+PVA
Sbjct: 8 IMALRKKLFESSNFNGKIMYTFSRYFSSNKMDLRKLKPMIQKRIQERAKDYPVPGMIPVA 67
Query: 80 RDVLKARALLIQGVSTLIKVFPVHAC 3
++VL +R LL+QGVSTL+KV PV AC
Sbjct: 68 QEVLNSRRLLMQGVSTLLKVLPVMAC 93
>emb|CAJ86368.1| OSIGBa0117N13.12 [Oryza sativa Indica Group]
gi|116312054|emb|CAJ86418.1| H0303G06.7 [Oryza sativa
Indica Group]
Length = 327
Score = 92.4 bits (228), Expect = 1e-16
Identities = 45/86 (52%), Positives = 65/86 (75%), Gaps = 5/86 (5%)
Frame = -2
Query: 245 MALRRK-----LCPNFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVA 81
MAL R+ +C G + + QR YSS+ D K+LRPMILKRI+NRAK+YP++ M+PVA
Sbjct: 1 MALMRRRVGGYICSELCGSVMN-QRLYSSRVDWKQLRPMILKRIKNRAKEYPIKRMIPVA 59
Query: 80 RDVLKARALLIQGVSTLIKVFPVHAC 3
+V+KAR ++ +GVSTL++V P+H+C
Sbjct: 60 EEVVKAREIVTKGVSTLLQVVPIHSC 85
>ref|NP_001053956.1| Os04g0628000 [Oryza sativa Japonica Group]
gi|113565527|dbj|BAF15870.1| Os04g0628000 [Oryza sativa
Japonica Group] gi|125549840|gb|EAY95662.1| hypothetical
protein OsI_17528 [Oryza sativa Indica Group]
gi|215693911|dbj|BAG89110.1| unnamed protein product
[Oryza sativa Japonica Group]
gi|222629605|gb|EEE61737.1| hypothetical protein
OsJ_16258 [Oryza sativa Japonica Group]
gi|937916217|dbj|BAS91136.1| Os04g0628000 [Oryza sativa
Japonica Group]
Length = 327
Score = 92.4 bits (228), Expect = 1e-16
Identities = 45/86 (52%), Positives = 65/86 (75%), Gaps = 5/86 (5%)
Frame = -2
Query: 245 MALRRK-----LCPNFMGELSSGQRFYSSKFDLKKLRPMILKRIENRAKDYPLRDMVPVA 81
MAL R+ +C G + + QR YSS+ D K+LRPMILKRI+NRAK+YP++ M+PVA
Sbjct: 1 MALMRRRVGGYICSELCGSVMN-QRLYSSRVDWKQLRPMILKRIKNRAKEYPIKRMIPVA 59
Query: 80 RDVLKARALLIQGVSTLIKVFPVHAC 3
+V+KAR ++ +GVSTL++V P+H+C
Sbjct: 60 EEVVKAREIVTKGVSTLLQVVPIHSC 85