BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Wisteria21_contig00038878
         (299 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012574911.1| PREDICTED: pentatricopeptide repeat-containi...   186   5e-45
ref|XP_013459241.1| pentatricopeptide (PPR) repeat protein [Medi...   184   2e-44
ref|XP_007148494.1| hypothetical protein PHAVU_006G213400g [Phas...   167   4e-39
gb|KRG99057.1| hypothetical protein GLYMA_18G117600 [Glycine max]     166   5e-39
gb|KHN28518.1| Pentatricopeptide repeat-containing protein [Glyc...   166   5e-39
ref|XP_003551954.1| PREDICTED: pentatricopeptide repeat-containi...   166   5e-39
gb|KOM52137.1| hypothetical protein LR48_Vigan09g079600 [Vigna a...   163   5e-38
ref|XP_014490303.1| PREDICTED: pentatricopeptide repeat-containi...   161   2e-37
gb|KHM99692.1| Pentatricopeptide repeat-containing protein [Glyc...   160   5e-37
ref|XP_003532067.1| PREDICTED: pentatricopeptide repeat-containi...   160   5e-37
ref|XP_007208151.1| hypothetical protein PRUPE_ppa022107mg [Prun...   159   8e-37
ref|XP_007146311.1| hypothetical protein PHAVU_006G029800g [Phas...   159   1e-36
ref|XP_008237400.1| PREDICTED: pentatricopeptide repeat-containi...   157   2e-36
emb|CBI29560.3| unnamed protein product [Vitis vinifera]              155   1e-35
ref|XP_002268807.2| PREDICTED: pentatricopeptide repeat-containi...   155   1e-35
emb|CAN74731.1| hypothetical protein VITISV_037837 [Vitis vinifera]   155   1e-35
ref|XP_008342220.1| PREDICTED: pentatricopeptide repeat-containi...   154   3e-35
ref|XP_004288283.1| PREDICTED: pentatricopeptide repeat-containi...   151   2e-34
ref|XP_009362618.1| PREDICTED: pentatricopeptide repeat-containi...   150   5e-34
ref|XP_004146400.1| PREDICTED: pentatricopeptide repeat-containi...   149   1e-33

>ref|XP_012574911.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           isoform X1 [Cicer arietinum]
           gi|828333203|ref|XP_012574912.1| PREDICTED:
           pentatricopeptide repeat-containing protein At3g49710
           isoform X2 [Cicer arietinum]
          Length = 722

 Score =  186 bits (473), Expect = 5e-45
 Identities = 90/99 (90%), Positives = 94/99 (94%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSG++R+ HVGSGLIDLYSKCAP GMLE
Sbjct: 238 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGFHRNSHVGSGLIDLYSKCAPHGMLE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           C KVFEEIP+PDLVLWNTMISGFSQHEDLSEDAL  FRE
Sbjct: 298 CMKVFEEIPKPDLVLWNTMISGFSQHEDLSEDALSSFRE 336


>ref|XP_013459241.1| pentatricopeptide (PPR) repeat protein [Medicago truncatula]
           gi|657392318|gb|KEH33272.1| pentatricopeptide (PPR)
           repeat protein [Medicago truncatula]
          Length = 724

 Score =  184 bits (468), Expect = 2e-44
 Identities = 89/99 (89%), Positives = 93/99 (93%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSG++R+ HVGSGLIDLYSKCAP GMLE
Sbjct: 239 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGFHRNSHVGSGLIDLYSKCAPHGMLE 298

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           C KVFEEIP+PDLVLWNTMISGFSQHEDL EDAL  FRE
Sbjct: 299 CMKVFEEIPKPDLVLWNTMISGFSQHEDLCEDALSSFRE 337


>ref|XP_007148494.1| hypothetical protein PHAVU_006G213400g [Phaseolus vulgaris]
           gi|561021717|gb|ESW20488.1| hypothetical protein
           PHAVU_006G213400g [Phaseolus vulgaris]
          Length = 722

 Score =  167 bits (422), Expect = 4e-39
 Identities = 80/99 (80%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GGMQFHG M+K+G+N + HVGSGLIDLYSKCA  GM+E
Sbjct: 239 LKVDMFTMASVLTAFTCVKDLVGGMQFHGMMVKTGFNGNSHVGSGLIDLYSKCA-AGMVE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFSQ+EDLSED L CF+E
Sbjct: 298 CRKVFEEIHAPDLVLWNTMISGFSQYEDLSEDGLWCFKE 336


>gb|KRG99057.1| hypothetical protein GLYMA_18G117600 [Glycine max]
          Length = 383

 Score =  166 bits (421), Expect = 5e-39
 Identities = 81/99 (81%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GGMQFHG MIKSG++ + HVGSGLIDLYSKCA  GM+E
Sbjct: 239 LKVDMFTMASVLTAFTCVKDLVGGMQFHGMMIKSGFHGNSHVGSGLIDLYSKCAG-GMVE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFSQ+EDLSED + CFRE
Sbjct: 298 CRKVFEEIAAPDLVLWNTMISGFSQYEDLSEDGIWCFRE 336


>gb|KHN28518.1| Pentatricopeptide repeat-containing protein [Glycine soja]
          Length = 523

 Score =  166 bits (421), Expect = 5e-39
 Identities = 81/99 (81%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GGMQFHG MIKSG++ + HVGSGLIDLYSKCA  GM+E
Sbjct: 40  LKVDMFTMASVLTAFTCVKDLVGGMQFHGMMIKSGFHGNSHVGSGLIDLYSKCAG-GMVE 98

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFSQ+EDLSED + CFRE
Sbjct: 99  CRKVFEEIAAPDLVLWNTMISGFSQYEDLSEDGIWCFRE 137


>ref|XP_003551954.1| PREDICTED: pentatricopeptide repeat-containing protein
           At3g49710-like [Glycine max]
          Length = 722

 Score =  166 bits (421), Expect = 5e-39
 Identities = 81/99 (81%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GGMQFHG MIKSG++ + HVGSGLIDLYSKCA  GM+E
Sbjct: 239 LKVDMFTMASVLTAFTCVKDLVGGMQFHGMMIKSGFHGNSHVGSGLIDLYSKCAG-GMVE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFSQ+EDLSED + CFRE
Sbjct: 298 CRKVFEEIAAPDLVLWNTMISGFSQYEDLSEDGIWCFRE 336


>gb|KOM52137.1| hypothetical protein LR48_Vigan09g079600 [Vigna angularis]
          Length = 722

 Score =  163 bits (412), Expect = 5e-38
 Identities = 79/99 (79%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GGMQFHG M+KSG++ + HVGSGLIDLYSKCA  GM+E
Sbjct: 239 LKVDMFTMASVLTAFTCVKDLVGGMQFHGMMVKSGFHGNSHVGSGLIDLYSKCAG-GMVE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVF+EI  PDLVLWNTMISGFSQ+EDLSED L CF+E
Sbjct: 298 CRKVFKEIRVPDLVLWNTMISGFSQYEDLSEDGLWCFQE 336


>ref|XP_014490303.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Vigna radiata var. radiata]
          Length = 722

 Score =  161 bits (408), Expect = 2e-37
 Identities = 78/99 (78%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GGMQFHG ++KSG++ + HVGSGLIDLYSKCA  GM+E
Sbjct: 239 LKVDMFTMASVLTAFTCVKDLVGGMQFHGMLVKSGFHGNSHVGSGLIDLYSKCAG-GMVE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVF+EI  PDLVLWNTMISGFSQ+EDLSED L CF+E
Sbjct: 298 CRKVFKEICVPDLVLWNTMISGFSQYEDLSEDGLWCFQE 336


>gb|KHM99692.1| Pentatricopeptide repeat-containing protein [Glycine soja]
          Length = 713

 Score =  160 bits (404), Expect = 5e-37
 Identities = 79/99 (79%), Positives = 86/99 (86%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GG QFHG MIKSG++ + HVGSGLIDLYSKCA   M+E
Sbjct: 230 LKVDMFTMASVLTAFTCVKDLVGGRQFHGMMIKSGFHGNSHVGSGLIDLYSKCAG-SMVE 288

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFS +EDLSED L CFRE
Sbjct: 289 CRKVFEEITAPDLVLWNTMISGFSLYEDLSEDGLWCFRE 327


>ref|XP_003532067.1| PREDICTED: pentatricopeptide repeat-containing protein
           At3g49710-like [Glycine max] gi|947097355|gb|KRH45940.1|
           hypothetical protein GLYMA_08G302300 [Glycine max]
          Length = 722

 Score =  160 bits (404), Expect = 5e-37
 Identities = 79/99 (79%), Positives = 86/99 (86%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL GG QFHG MIKSG++ + HVGSGLIDLYSKCA   M+E
Sbjct: 239 LKVDMFTMASVLTAFTCVKDLVGGRQFHGMMIKSGFHGNSHVGSGLIDLYSKCAG-SMVE 297

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFS +EDLSED L CFRE
Sbjct: 298 CRKVFEEITAPDLVLWNTMISGFSLYEDLSEDGLWCFRE 336


>ref|XP_007208151.1| hypothetical protein PRUPE_ppa022107mg [Prunus persica]
           gi|462403793|gb|EMJ09350.1| hypothetical protein
           PRUPE_ppa022107mg [Prunus persica]
          Length = 720

 Score =  159 bits (402), Expect = 8e-37
 Identities = 72/99 (72%), Positives = 90/99 (90%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFT+ASVLTAFTC++DL GG+QFH K+IK+G++++ HVGSGLIDLYSKCA  GM +
Sbjct: 236 LKVDMFTLASVLTAFTCVEDLLGGLQFHAKLIKTGFHQNSHVGSGLIDLYSKCAAGGMSD 295

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRK+FEEIP PDLVLWNTMISG+SQ+++ SEDAL CFR+
Sbjct: 296 CRKLFEEIPYPDLVLWNTMISGYSQNDEFSEDALDCFRQ 334


>ref|XP_007146311.1| hypothetical protein PHAVU_006G029800g [Phaseolus vulgaris]
           gi|561019534|gb|ESW18305.1| hypothetical protein
           PHAVU_006G029800g [Phaseolus vulgaris]
          Length = 665

 Score =  159 bits (401), Expect = 1e-36
 Identities = 77/99 (77%), Positives = 87/99 (87%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L+VDMFTMASVLTAFTC+KDL   MQFHG M+K+G++ + HVGSGLIDLYSKCA  GM+E
Sbjct: 196 LKVDMFTMASVLTAFTCVKDLVVDMQFHGMMVKTGFHGNSHVGSGLIDLYSKCA-AGMVE 254

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI  PDLVLWNTMISGFSQ+EDLSED L CF+E
Sbjct: 255 CRKVFEEIHAPDLVLWNTMISGFSQYEDLSEDGLWCFKE 293


>ref|XP_008237400.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Prunus mume]
          Length = 720

 Score =  157 bits (398), Expect = 2e-36
 Identities = 71/98 (72%), Positives = 89/98 (90%)
 Frame = -2

Query: 295 EVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLEC 116
           +VDMFT+ASVLTAFTC++DL GG+QFH K+IK+G++++ HVGSGLIDLYSKCA  GM +C
Sbjct: 237 KVDMFTLASVLTAFTCVEDLLGGLQFHAKLIKTGFHQNSHVGSGLIDLYSKCAAGGMSDC 296

Query: 115 RKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           RK+FEEIP PDLVLWNTMISG+SQ+++ SEDAL CFR+
Sbjct: 297 RKLFEEIPYPDLVLWNTMISGYSQNDEFSEDALDCFRQ 334


>emb|CBI29560.3| unnamed protein product [Vitis vinifera]
          Length = 640

 Score =  155 bits (391), Expect = 1e-35
 Identities = 72/99 (72%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L VDMFT+ASVLTAFTCL+DL+GG+QFHG++IK+G++++ HVGSGLIDLYSKC   GM +
Sbjct: 236 LNVDMFTLASVLTAFTCLEDLSGGLQFHGQLIKTGFHQNSHVGSGLIDLYSKCGG-GMSD 294

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI EPDLVLWNTM+SG+SQ+E+  EDAL CFR+
Sbjct: 295 CRKVFEEITEPDLVLWNTMVSGYSQNEEFLEDALECFRQ 333


>ref|XP_002268807.2| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Vitis vinifera]
          Length = 719

 Score =  155 bits (391), Expect = 1e-35
 Identities = 72/99 (72%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L VDMFT+ASVLTAFTCL+DL+GG+QFHG++IK+G++++ HVGSGLIDLYSKC   GM +
Sbjct: 236 LNVDMFTLASVLTAFTCLEDLSGGLQFHGQLIKTGFHQNSHVGSGLIDLYSKCGG-GMSD 294

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI EPDLVLWNTM+SG+SQ+E+  EDAL CFR+
Sbjct: 295 CRKVFEEITEPDLVLWNTMVSGYSQNEEFLEDALECFRQ 333


>emb|CAN74731.1| hypothetical protein VITISV_037837 [Vitis vinifera]
          Length = 719

 Score =  155 bits (391), Expect = 1e-35
 Identities = 72/99 (72%), Positives = 89/99 (89%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L VDMFT+ASVLTAFTCL+DL+GG+QFHG++IK+G++++ HVGSGLIDLYSKC   GM +
Sbjct: 236 LNVDMFTLASVLTAFTCLEDLSGGLQFHGQLIKTGFHQNSHVGSGLIDLYSKCGG-GMSD 294

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVFEEI EPDLVLWNTM+SG+SQ+E+  EDAL CFR+
Sbjct: 295 CRKVFEEITEPDLVLWNTMVSGYSQNEEFLEDALECFRQ 333


>ref|XP_008342220.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Malus domestica]
          Length = 719

 Score =  154 bits (389), Expect = 3e-35
 Identities = 71/98 (72%), Positives = 88/98 (89%)
 Frame = -2

Query: 295 EVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLEC 116
           +VDMFT+ASVLTAFTC++DL GG+QFH K+IK+G++++ HVGSGLIDLYSKCA  GM EC
Sbjct: 237 DVDMFTLASVLTAFTCVEDLLGGLQFHAKLIKTGFHQNSHVGSGLIDLYSKCAG-GMAEC 295

Query: 115 RKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           RKVF+EIP PDLVLWNTM+SG+SQ+ + SEDAL CFR+
Sbjct: 296 RKVFQEIPXPDLVLWNTMVSGYSQNAEFSEDALHCFRK 333


>ref|XP_004288283.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Fragaria vesca subsp. vesca]
          Length = 719

 Score =  151 bits (382), Expect = 2e-34
 Identities = 71/96 (73%), Positives = 85/96 (88%)
 Frame = -2

Query: 292 VDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLECR 113
           VDMFT+ASVLTAFTC++DL GG+QFH K+IK+G++++ HVGSGLIDLYSKCA  GM ECR
Sbjct: 238 VDMFTLASVLTAFTCVEDLLGGLQFHAKLIKTGFHQNSHVGSGLIDLYSKCAG-GMSECR 296

Query: 112 KVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFR 5
           KVFEEIP PDLVLWNTMISG+SQ ++ SEDA+ C R
Sbjct: 297 KVFEEIPAPDLVLWNTMISGYSQSDEFSEDAIDCLR 332


>ref|XP_009362618.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Pyrus x bretschneideri]
          Length = 719

 Score =  150 bits (378), Expect = 5e-34
 Identities = 70/97 (72%), Positives = 87/97 (89%)
 Frame = -2

Query: 292 VDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLECR 113
           VDMFT+ASVLTAFTC++DL GG+QFH K+IK+G++++ HVGSGLIDLYSKCA  GM EC 
Sbjct: 238 VDMFTLASVLTAFTCVEDLLGGLQFHAKLIKTGFHQNSHVGSGLIDLYSKCAG-GMAECG 296

Query: 112 KVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           KVF+EIP PDLVLWNT+ISG+SQ+++ SEDAL CFR+
Sbjct: 297 KVFQEIPFPDLVLWNTIISGYSQNDEFSEDALHCFRK 333


>ref|XP_004146400.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710
           [Cucumis sativus] gi|700199669|gb|KGN54827.1|
           hypothetical protein Csa_4G508540 [Cucumis sativus]
          Length = 720

 Score =  149 bits (375), Expect = 1e-33
 Identities = 71/99 (71%), Positives = 87/99 (87%)
 Frame = -2

Query: 298 LEVDMFTMASVLTAFTCLKDLAGGMQFHGKMIKSGYNRSPHVGSGLIDLYSKCAPCGMLE 119
           L VD+FT+ASVLTAFT ++DL GG+QFH K+IKSGY+++ HVGSGLIDLYSKC  C ML+
Sbjct: 236 LIVDIFTLASVLTAFTNVQDLLGGLQFHAKLIKSGYHQNSHVGSGLIDLYSKCGGC-MLD 294

Query: 118 CRKVFEEIPEPDLVLWNTMISGFSQHEDLSEDALCCFRE 2
           CRKVF+EI  PDLVLWNTMISG+S +EDLS++AL CFR+
Sbjct: 295 CRKVFDEISNPDLVLWNTMISGYSLYEDLSDEALECFRQ 333