BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Wisteria21_contig00028124
         (503 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004488267.1| PREDICTED: vacuolar protein sorting-associat...   157   2e-36
ref|XP_013463768.1| vacuolar sorting protein [Medicago truncatul...   147   4e-33
ref|XP_003547564.1| PREDICTED: vacuolar protein sorting-associat...   137   4e-30
gb|KRH37714.1| hypothetical protein GLYMA_09G084100 [Glycine max]     134   3e-29
gb|KRH37713.1| hypothetical protein GLYMA_09G084100 [Glycine max]     134   3e-29
ref|XP_003533830.1| PREDICTED: vacuolar protein sorting-associat...   134   3e-29
ref|XP_007022899.1| VPS54 isoform 6 [Theobroma cacao] gi|5087782...   134   3e-29
ref|XP_007022898.1| VPS54 isoform 5 [Theobroma cacao] gi|5087782...   134   3e-29
ref|XP_007022897.1| VPS54 isoform 4 [Theobroma cacao] gi|5087782...   134   3e-29
ref|XP_007022895.1| VPS54 isoform 2 [Theobroma cacao] gi|5906142...   134   3e-29
ref|XP_007022894.1| VPS54 isoform 1 [Theobroma cacao] gi|5087782...   134   3e-29
gb|KHF97631.1| Vacuolar sorting-associated protein 54 [Gossypium...   131   2e-28
ref|XP_007138542.1| hypothetical protein PHAVU_009G217900g [Phas...   130   4e-28
ref|XP_014501138.1| PREDICTED: vacuolar protein sorting-associat...   130   5e-28
gb|KOM24798.1| hypothetical protein LR48_Vigan2540s000100 [Vigna...   129   6e-28
ref|XP_012454025.1| PREDICTED: vacuolar protein sorting-associat...   129   6e-28
ref|XP_010109308.1| Vacuolar protein sorting-associated protein ...   129   1e-27
ref|XP_008439467.1| PREDICTED: vacuolar protein sorting-associat...   125   1e-26
ref|XP_004134530.1| PREDICTED: vacuolar protein sorting-associat...   124   3e-26
emb|CAN79629.1| hypothetical protein VITISV_002584 [Vitis vinifera]   121   2e-25

>ref|XP_004488267.1| PREDICTED: vacuolar protein sorting-associated protein 54,
           chloroplastic [Cicer arietinum]
          Length = 997

 Score =  157 bits (398), Expect = 2e-36
 Identities = 88/151 (58%), Positives = 100/151 (66%), Gaps = 4/151 (2%)
 Frame = -2

Query: 442 MNSPP----WGRSPTXXXXXXXXXXXXXXXXXXXXXXXXXSDANAIQXXXXXXXXXXXSA 275
           M+SPP    WGRSPT                            +AIQ           S 
Sbjct: 1   MDSPPSQQTWGRSPTLSTHLNRTTSSASSTSSPSF------SKDAIQSLSSILNNPLSST 54

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           TIS PEF+PI S+K+AS+V RSDF+PY+S ++D FHRFDDIRKH+TKEISADVD  GAGE
Sbjct: 55  TISSPEFSPIVSTKSASEVTRSDFIPYLSTVSDPFHRFDDIRKHSTKEISADVD--GAGE 112

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVPSLYFKEDFRLE+GATFRAACP
Sbjct: 113 ALVACLREVPSLYFKEDFRLEEGATFRAACP 143


>ref|XP_013463768.1| vacuolar sorting protein [Medicago truncatula]
           gi|657398181|gb|KEH37803.1| vacuolar sorting protein
           [Medicago truncatula]
          Length = 1001

 Score =  147 bits (370), Expect = 4e-33
 Identities = 79/149 (53%), Positives = 90/149 (60%)
 Frame = -2

Query: 448 PIMNSPPWGRSPTXXXXXXXXXXXXXXXXXXXXXXXXXSDANAIQXXXXXXXXXXXSATI 269
           P    P WGRSPT                          D++ IQ           S TI
Sbjct: 4   PPSQQPSWGRSPTFSTTHLNRTTSTSSSTSSPSFSK---DSSTIQSLSSILNNPLSSTTI 60

Query: 268 SPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGEAL 89
           S PEF PISS+K+ S + RSDFLPY+S ++D FHRFD+IRKHA  EIS   D  GAGEAL
Sbjct: 61  SSPEFTPISSTKSTSTLTRSDFLPYLSSLSDPFHRFDEIRKHANNEISTISDDGGAGEAL 120

Query: 88  VACLREVPSLYFKEDFRLEDGATFRAACP 2
           VACLREVPSLYFK+DF LE+GATFRAACP
Sbjct: 121 VACLREVPSLYFKQDFHLEEGATFRAACP 149


>ref|XP_003547564.1| PREDICTED: vacuolar protein sorting-associated protein 54-like
           [Glycine max] gi|947063494|gb|KRH12755.1| hypothetical
           protein GLYMA_15G192300 [Glycine max]
          Length = 1029

 Score =  137 bits (344), Expect = 4e-30
 Identities = 81/160 (50%), Positives = 97/160 (60%), Gaps = 13/160 (8%)
 Frame = -2

Query: 442 MNSPP----WGRSPTXXXXXXXXXXXXXXXXXXXXXXXXXSDANAIQXXXXXXXXXXXSA 275
           M+SPP    WGRSPT                          + +A             ++
Sbjct: 1   MDSPPSQHSWGRSPTSLSSSSSSLSKSNSDTIQSLSSILN-NPHASDAASWAGWWSSSAS 59

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKE------ISADVD 113
            +S PEFAPIS+SKAASDV+RSDFLPY+S IAD+FHRF DIR HA+ E       +AD  
Sbjct: 60  AVSVPEFAPISASKAASDVSRSDFLPYLSPIADAFHRFADIRNHASNEQINAAAATADAT 119

Query: 112 GAGA---GEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
            +G+   G+ALVACLREVP+LYFKEDFRLEDGATFRAACP
Sbjct: 120 NSGSVGQGKALVACLREVPALYFKEDFRLEDGATFRAACP 159


>gb|KRH37714.1| hypothetical protein GLYMA_09G084100 [Glycine max]
          Length = 384

 Score =  134 bits (337), Expect = 3e-29
 Identities = 69/100 (69%), Positives = 80/100 (80%), Gaps = 10/100 (10%)
 Frame = -2

Query: 271 ISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKE-ISADVD------ 113
           ++ PEFA I +SKAASDV+RSDFLPY+S I+D+FHRF+DIR HA+ E I+A  D      
Sbjct: 60  VAVPEFAIIPASKAASDVSRSDFLPYLSPISDAFHRFEDIRNHASNEQINASADAATNST 119

Query: 112 ---GAGAGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
              G G GEALVACLREVP+LYFKEDFRLEDGATFRAACP
Sbjct: 120 GSGGGGQGEALVACLREVPALYFKEDFRLEDGATFRAACP 159


>gb|KRH37713.1| hypothetical protein GLYMA_09G084100 [Glycine max]
          Length = 363

 Score =  134 bits (337), Expect = 3e-29
 Identities = 69/100 (69%), Positives = 80/100 (80%), Gaps = 10/100 (10%)
 Frame = -2

Query: 271 ISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKE-ISADVD------ 113
           ++ PEFA I +SKAASDV+RSDFLPY+S I+D+FHRF+DIR HA+ E I+A  D      
Sbjct: 60  VAVPEFAIIPASKAASDVSRSDFLPYLSPISDAFHRFEDIRNHASNEQINASADAATNST 119

Query: 112 ---GAGAGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
              G G GEALVACLREVP+LYFKEDFRLEDGATFRAACP
Sbjct: 120 GSGGGGQGEALVACLREVPALYFKEDFRLEDGATFRAACP 159


>ref|XP_003533830.1| PREDICTED: vacuolar protein sorting-associated protein 54-like
           [Glycine max]
          Length = 1029

 Score =  134 bits (337), Expect = 3e-29
 Identities = 69/100 (69%), Positives = 80/100 (80%), Gaps = 10/100 (10%)
 Frame = -2

Query: 271 ISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKE-ISADVD------ 113
           ++ PEFA I +SKAASDV+RSDFLPY+S I+D+FHRF+DIR HA+ E I+A  D      
Sbjct: 60  VAVPEFAIIPASKAASDVSRSDFLPYLSPISDAFHRFEDIRNHASNEQINASADAATNST 119

Query: 112 ---GAGAGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
              G G GEALVACLREVP+LYFKEDFRLEDGATFRAACP
Sbjct: 120 GSGGGGQGEALVACLREVPALYFKEDFRLEDGATFRAACP 159


>ref|XP_007022899.1| VPS54 isoform 6 [Theobroma cacao] gi|508778265|gb|EOY25521.1| VPS54
           isoform 6 [Theobroma cacao]
          Length = 956

 Score =  134 bits (336), Expect = 3e-29
 Identities = 63/91 (69%), Positives = 77/91 (84%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           ++SPPEFAP+ S+KA+SD+ RSDF  Y+S ++DS+HRF+DIR H+TKE + DVD    GE
Sbjct: 51  SVSPPEFAPLISTKASSDLTRSDFQSYVSSVSDSYHRFEDIRNHSTKEQTLDVD--NIGE 108

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 109 ALVACLREVPALYFKEDFALEDGPTFRAACP 139


>ref|XP_007022898.1| VPS54 isoform 5 [Theobroma cacao] gi|508778264|gb|EOY25520.1| VPS54
           isoform 5 [Theobroma cacao]
          Length = 757

 Score =  134 bits (336), Expect = 3e-29
 Identities = 63/91 (69%), Positives = 77/91 (84%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           ++SPPEFAP+ S+KA+SD+ RSDF  Y+S ++DS+HRF+DIR H+TKE + DVD    GE
Sbjct: 51  SVSPPEFAPLISTKASSDLTRSDFQSYVSSVSDSYHRFEDIRNHSTKEQTLDVD--NIGE 108

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 109 ALVACLREVPALYFKEDFALEDGPTFRAACP 139


>ref|XP_007022897.1| VPS54 isoform 4 [Theobroma cacao] gi|508778263|gb|EOY25519.1| VPS54
           isoform 4 [Theobroma cacao]
          Length = 849

 Score =  134 bits (336), Expect = 3e-29
 Identities = 63/91 (69%), Positives = 77/91 (84%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           ++SPPEFAP+ S+KA+SD+ RSDF  Y+S ++DS+HRF+DIR H+TKE + DVD    GE
Sbjct: 51  SVSPPEFAPLISTKASSDLTRSDFQSYVSSVSDSYHRFEDIRNHSTKEQTLDVD--NIGE 108

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 109 ALVACLREVPALYFKEDFALEDGPTFRAACP 139


>ref|XP_007022895.1| VPS54 isoform 2 [Theobroma cacao] gi|590614288|ref|XP_007022896.1|
           VPS54 isoform 2 [Theobroma cacao]
           gi|508778261|gb|EOY25517.1| VPS54 isoform 2 [Theobroma
           cacao] gi|508778262|gb|EOY25518.1| VPS54 isoform 2
           [Theobroma cacao]
          Length = 1001

 Score =  134 bits (336), Expect = 3e-29
 Identities = 63/91 (69%), Positives = 77/91 (84%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           ++SPPEFAP+ S+KA+SD+ RSDF  Y+S ++DS+HRF+DIR H+TKE + DVD    GE
Sbjct: 51  SVSPPEFAPLISTKASSDLTRSDFQSYVSSVSDSYHRFEDIRNHSTKEQTLDVD--NIGE 108

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 109 ALVACLREVPALYFKEDFALEDGPTFRAACP 139


>ref|XP_007022894.1| VPS54 isoform 1 [Theobroma cacao] gi|508778260|gb|EOY25516.1| VPS54
           isoform 1 [Theobroma cacao]
          Length = 1002

 Score =  134 bits (336), Expect = 3e-29
 Identities = 63/91 (69%), Positives = 77/91 (84%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           ++SPPEFAP+ S+KA+SD+ RSDF  Y+S ++DS+HRF+DIR H+TKE + DVD    GE
Sbjct: 51  SVSPPEFAPLISTKASSDLTRSDFQSYVSSVSDSYHRFEDIRNHSTKEQTLDVD--NIGE 108

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 109 ALVACLREVPALYFKEDFALEDGPTFRAACP 139


>gb|KHF97631.1| Vacuolar sorting-associated protein 54 [Gossypium arboreum]
          Length = 972

 Score =  131 bits (329), Expect = 2e-28
 Identities = 62/91 (68%), Positives = 76/91 (83%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           +++PPEFAP++S+KAA D+ RSDF  Y+S I+DS++RF+DIR H TKE + DVD    GE
Sbjct: 50  SVAPPEFAPLTSTKAACDLTRSDFQSYVSSISDSYYRFEDIRNHTTKEQTLDVD--NIGE 107

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 108 ALVACLREVPALYFKEDFALEDGGTFRAACP 138


>ref|XP_007138542.1| hypothetical protein PHAVU_009G217900g [Phaseolus vulgaris]
           gi|561011629|gb|ESW10536.1| hypothetical protein
           PHAVU_009G217900g [Phaseolus vulgaris]
          Length = 1026

 Score =  130 bits (327), Expect = 4e-28
 Identities = 63/96 (65%), Positives = 79/96 (82%), Gaps = 4/96 (4%)
 Frame = -2

Query: 277 ATISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGA--- 107
           + ++PPEFAPI+++KAAS+V+RSDF  Y+  IA+++HRF+DIR H +KE   D+  A   
Sbjct: 61  SAVAPPEFAPIAAAKAASEVSRSDFQHYVVPIAEAYHRFEDIRNHTSKEQINDLANAAAS 120

Query: 106 -GAGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
            G GEALVACLREVP+LYFKEDFRLEDGATFRAACP
Sbjct: 121 SGQGEALVACLREVPALYFKEDFRLEDGATFRAACP 156


>ref|XP_014501138.1| PREDICTED: vacuolar protein sorting-associated protein 54,
           chloroplastic [Vigna radiata var. radiata]
          Length = 1019

 Score =  130 bits (326), Expect = 5e-28
 Identities = 63/94 (67%), Positives = 79/94 (84%), Gaps = 4/94 (4%)
 Frame = -2

Query: 271 ISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGA----G 104
           ++PPEFAPI+ +KAAS+V+RSDF  Y++ IA+++HRF+DIR H++KE   D+  A    G
Sbjct: 59  VAPPEFAPIAVAKAASEVSRSDFHNYVAPIAEAYHRFEDIRNHSSKEQINDLANAAAASG 118

Query: 103 AGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
            GEALVACLREVP+LYFKEDFRLEDGATFRAACP
Sbjct: 119 QGEALVACLREVPALYFKEDFRLEDGATFRAACP 152


>gb|KOM24798.1| hypothetical protein LR48_Vigan2540s000100 [Vigna angularis]
          Length = 320

 Score =  129 bits (325), Expect = 6e-28
 Identities = 64/94 (68%), Positives = 77/94 (81%), Gaps = 4/94 (4%)
 Frame = -2

Query: 271 ISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGA----G 104
           ++PPEFAPI+ +KAAS+V+RSDF  Y+  IA+++HRF+DIR H +KE   D+  A    G
Sbjct: 59  VAPPEFAPIAVAKAASEVSRSDFHNYVVPIAEAYHRFEDIRNHTSKEQINDLANAAAASG 118

Query: 103 AGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
            GEALVACLREVPSLYFKEDFRLEDGATFRAACP
Sbjct: 119 QGEALVACLREVPSLYFKEDFRLEDGATFRAACP 152


>ref|XP_012454025.1| PREDICTED: vacuolar protein sorting-associated protein 54,
           chloroplastic [Gossypium raimondii]
           gi|763802148|gb|KJB69086.1| hypothetical protein
           B456_011G004800 [Gossypium raimondii]
           gi|763802149|gb|KJB69087.1| hypothetical protein
           B456_011G004800 [Gossypium raimondii]
          Length = 991

 Score =  129 bits (325), Expect = 6e-28
 Identities = 61/91 (67%), Positives = 75/91 (82%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAGE 95
           +++PPEF P++S+KAA D+ RSDF  Y+S I+DS++RF+DIR H TKE + DVD    GE
Sbjct: 50  SVAPPEFTPLTSTKAACDLTRSDFQSYVSSISDSYYRFEDIRNHTTKEQTLDVD--NIGE 107

Query: 94  ALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           ALVACLREVP+LYFKEDF LEDG TFRAACP
Sbjct: 108 ALVACLREVPALYFKEDFALEDGGTFRAACP 138


>ref|XP_010109308.1| Vacuolar protein sorting-associated protein 54 [Morus notabilis]
           gi|587934837|gb|EXC21740.1| Vacuolar protein
           sorting-associated protein 54 [Morus notabilis]
          Length = 995

 Score =  129 bits (323), Expect = 1e-27
 Identities = 63/92 (68%), Positives = 80/92 (86%), Gaps = 1/92 (1%)
 Frame = -2

Query: 274 TISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGA-G 98
           +++ PEFAP+SS KAASDV+RSDF PY++ I++ +HRF+D+R H++KE S D+DG G  G
Sbjct: 61  SVAAPEFAPLSS-KAASDVSRSDFQPYVASISEPYHRFEDVRNHSSKE-SLDLDGIGGQG 118

Query: 97  EALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           EALVACLREVP+LYFKEDF LEDGATFR+ACP
Sbjct: 119 EALVACLREVPALYFKEDFALEDGATFRSACP 150


>ref|XP_008439467.1| PREDICTED: vacuolar protein sorting-associated protein 54,
           chloroplastic [Cucumis melo]
          Length = 1014

 Score =  125 bits (314), Expect = 1e-26
 Identities = 60/92 (65%), Positives = 74/92 (80%)
 Frame = -2

Query: 277 ATISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAG 98
           +T++PPEF P+SSS A+S+V R DF  Y ++I+DSFHRF+DIR H++KE        G G
Sbjct: 63  STVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDSIGGQG 122

Query: 97  EALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           EALVACLREVP+LYFKEDF LE+GATFRAACP
Sbjct: 123 EALVACLREVPALYFKEDFALEEGATFRAACP 154


>ref|XP_004134530.1| PREDICTED: vacuolar protein sorting-associated protein 54,
           chloroplastic [Cucumis sativus]
           gi|700194318|gb|KGN49522.1| hypothetical protein
           Csa_6G526560 [Cucumis sativus]
          Length = 1014

 Score =  124 bits (311), Expect = 3e-26
 Identities = 59/92 (64%), Positives = 74/92 (80%)
 Frame = -2

Query: 277 ATISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKEISADVDGAGAG 98
           +T++PPEF P+SS+ A+S+V R DF  Y ++I+DSFHRF+DIR H++KE        G G
Sbjct: 63  STVNPPEFMPLSSTIASSEVTRFDFNNYTALISDSFHRFEDIRNHSSKENGGLDSIGGQG 122

Query: 97  EALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           EALVACLREVP+LYFKEDF LE+GATFRAACP
Sbjct: 123 EALVACLREVPALYFKEDFALEEGATFRAACP 154


>emb|CAN79629.1| hypothetical protein VITISV_002584 [Vitis vinifera]
          Length = 964

 Score =  121 bits (304), Expect = 2e-25
 Identities = 60/98 (61%), Positives = 76/98 (77%), Gaps = 8/98 (8%)
 Frame = -2

Query: 271 ISPPEFAPISSSKAASDVARSDFLPYISVIADSFHRFDDIRKHATKE--------ISADV 116
           +SPPEF+P+ S KA+S+VARSDF PY++ I++ + RF+DIR H +KE        +S + 
Sbjct: 75  VSPPEFSPLVSGKASSEVARSDFQPYLASISEPYGRFEDIRNHKSKENGELEGFGMSKNG 134

Query: 115 DGAGAGEALVACLREVPSLYFKEDFRLEDGATFRAACP 2
           +  G GEALVACLREVPSLYFKEDF LE+GATFRAACP
Sbjct: 135 EIQGQGEALVACLREVPSLYFKEDFALEEGATFRAACP 172