BLASTX nr result
ID: Wisteria21_contig00016763
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Wisteria21_contig00016763 (360 letters) Database: ./nr 77,306,371 sequences; 28,104,191,420 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|KRH73819.1| hypothetical protein GLYMA_02G295200 [Glycine max] 163 5e-38 gb|KRH73818.1| hypothetical protein GLYMA_02G295200 [Glycine max] 163 5e-38 ref|XP_003519599.1| PREDICTED: vacuolar protein sorting-associat... 163 5e-38 gb|KHN38239.1| Vacuolar protein sorting-associated protein VTA1 ... 160 5e-37 gb|KHN23888.1| Vacuolar protein sorting-associated protein VTA1 ... 159 8e-37 ref|XP_003545111.1| PREDICTED: vacuolar protein sorting-associat... 159 8e-37 ref|XP_003617645.1| hydroxyproline-rich glycoprotein family prot... 157 4e-36 ref|XP_014502520.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST... 155 9e-36 ref|XP_007142345.1| hypothetical protein PHAVU_008G272700g [Phas... 152 1e-34 gb|KOM46565.1| hypothetical protein LR48_Vigan07g026900 [Vigna a... 148 1e-33 ref|XP_004491432.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST... 114 4e-23 ref|XP_002531202.1| Protein C6orf55, putative [Ricinus communis]... 99 1e-18 ref|XP_007020695.1| Hydroxyproline-rich glycoprotein family prot... 88 2e-15 ref|XP_011013801.1| PREDICTED: vacuolar protein sorting-associat... 81 3e-13 ref|XP_010096091.1| hypothetical protein L484_012446 [Morus nota... 81 4e-13 ref|XP_004300450.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST... 80 5e-13 ref|XP_006377238.1| hypothetical protein POPTR_0011s02470g [Popu... 77 4e-12 ref|XP_012461261.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST... 77 5e-12 ref|XP_008235151.1| PREDICTED: vacuolar protein sorting-associat... 76 9e-12 ref|XP_012070918.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST... 74 3e-11 >gb|KRH73819.1| hypothetical protein GLYMA_02G295200 [Glycine max] Length = 401 Score = 163 bits (412), Expect = 5e-38 Identities = 79/108 (73%), Positives = 86/108 (79%), Gaps = 1/108 (0%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHP-APK 183 GPPDGDEDLSVP S+ S+RYDLGTTENT +S G ESDS+ SY N NYQNLPSIHP APK Sbjct: 89 GPPDGDEDLSVPLSSSSDRYDLGTTENTVSSPGPESDSSRSYHNPANYQNLPSIHPAAPK 148 Query: 182 FHDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 FHDT NDQHSANIPPS+ FHDRVD+NKH S+TP VYPSQD Sbjct: 149 FHDTVNDQHSANIPPSMPFHDRVDNNKHSSVVSPSSHSFTPGVYPSQD 196 >gb|KRH73818.1| hypothetical protein GLYMA_02G295200 [Glycine max] Length = 360 Score = 163 bits (412), Expect = 5e-38 Identities = 79/108 (73%), Positives = 86/108 (79%), Gaps = 1/108 (0%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHP-APK 183 GPPDGDEDLSVP S+ S+RYDLGTTENT +S G ESDS+ SY N NYQNLPSIHP APK Sbjct: 155 GPPDGDEDLSVPLSSSSDRYDLGTTENTVSSPGPESDSSRSYHNPANYQNLPSIHPAAPK 214 Query: 182 FHDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 FHDT NDQHSANIPPS+ FHDRVD+NKH S+TP VYPSQD Sbjct: 215 FHDTVNDQHSANIPPSMPFHDRVDNNKHSSVVSPSSHSFTPGVYPSQD 262 >ref|XP_003519599.1| PREDICTED: vacuolar protein sorting-associated protein VTA1 homolog [Glycine max] gi|947125611|gb|KRH73817.1| hypothetical protein GLYMA_02G295200 [Glycine max] Length = 467 Score = 163 bits (412), Expect = 5e-38 Identities = 79/108 (73%), Positives = 86/108 (79%), Gaps = 1/108 (0%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHP-APK 183 GPPDGDEDLSVP S+ S+RYDLGTTENT +S G ESDS+ SY N NYQNLPSIHP APK Sbjct: 155 GPPDGDEDLSVPLSSSSDRYDLGTTENTVSSPGPESDSSRSYHNPANYQNLPSIHPAAPK 214 Query: 182 FHDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 FHDT NDQHSANIPPS+ FHDRVD+NKH S+TP VYPSQD Sbjct: 215 FHDTVNDQHSANIPPSMPFHDRVDNNKHSSVVSPSSHSFTPGVYPSQD 262 >gb|KHN38239.1| Vacuolar protein sorting-associated protein VTA1 like [Glycine soja] Length = 467 Score = 160 bits (404), Expect = 5e-37 Identities = 78/108 (72%), Positives = 85/108 (78%), Gaps = 1/108 (0%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHP-APK 183 GPPDGDEDLSVP S+ S+RYDLGTTENT +S G ESDS+ SY N NYQNLPSIHP APK Sbjct: 155 GPPDGDEDLSVPLSSSSDRYDLGTTENTVSSPGPESDSSRSYHNPANYQNLPSIHPAAPK 214 Query: 182 FHDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 FHDT NDQHSANIP S+ FHDRVD+NKH S+TP VYPSQD Sbjct: 215 FHDTVNDQHSANIPSSMPFHDRVDNNKHSSVVSPSSHSFTPGVYPSQD 262 >gb|KHN23888.1| Vacuolar protein sorting-associated protein VTA1 like [Glycine soja] Length = 523 Score = 159 bits (402), Expect = 8e-37 Identities = 76/107 (71%), Positives = 84/107 (78%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPPDGDEDLSVP S+ S+RYDLGTTE T +S G ESDS+HSY + VNYQNLPSI P PKF Sbjct: 155 GPPDGDEDLSVPLSSSSDRYDLGTTETTVSSPGPESDSSHSYHSPVNYQNLPSIQPTPKF 214 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HDT NDQHSA+IPPS+ FHDRVD+NKH SYT VYPSQD Sbjct: 215 HDTVNDQHSADIPPSMPFHDRVDNNKHSSVVSPSSHSYTLGVYPSQD 261 >ref|XP_003545111.1| PREDICTED: vacuolar protein sorting-associated protein VTA1 homolog [Glycine max] gi|947065167|gb|KRH14310.1| hypothetical protein GLYMA_14G018200 [Glycine max] Length = 487 Score = 159 bits (402), Expect = 8e-37 Identities = 76/107 (71%), Positives = 84/107 (78%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPPDGDEDLSVP S+ S+RYDLGTTE T +S G ESDS+HSY + VNYQNLPSI P PKF Sbjct: 155 GPPDGDEDLSVPLSSSSDRYDLGTTETTVSSPGPESDSSHSYHSPVNYQNLPSIQPTPKF 214 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HDT NDQHSA+IPPS+ FHDRVD+NKH SYT VYPSQD Sbjct: 215 HDTVNDQHSADIPPSMPFHDRVDNNKHSSVVSPSSHSYTLGVYPSQD 261 >ref|XP_003617645.1| hydroxyproline-rich glycoprotein family protein [Medicago truncatula] gi|355518980|gb|AET00604.1| hydroxyproline-rich glycoprotein family protein [Medicago truncatula] Length = 487 Score = 157 bits (396), Expect = 4e-36 Identities = 76/107 (71%), Positives = 84/107 (78%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GDEDLSV S+PSN YD+GTTE + + GSESDSTH Y N VNYQNLPSI PAPKF Sbjct: 154 GPPAGDEDLSVLSSSPSNIYDIGTTETSASGAGSESDSTHGYHNPVNYQNLPSIPPAPKF 213 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 +DT NDQ+SANIPPS+QFHDRV+DNKH SYTP VYPSQD Sbjct: 214 NDTLNDQNSANIPPSLQFHDRVNDNKHSSIVSPSSHSYTPGVYPSQD 260 >ref|XP_014502520.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5 [Vigna radiata var. radiata] Length = 487 Score = 155 bits (393), Expect = 9e-36 Identities = 75/107 (70%), Positives = 84/107 (78%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GDEDLS+PFS+ S RYD GTTE T ++ G ESDS+ SY N VNYQN P+IHPAPKF Sbjct: 155 GPPAGDEDLSIPFSSSSGRYDPGTTETTVSNPGPESDSSPSYHNPVNYQN-PNIHPAPKF 213 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HDT N+QHSANIPPS+QFHDRVD+NKH SYTP VYPSQD Sbjct: 214 HDTVNEQHSANIPPSMQFHDRVDNNKHSSVVSPSSHSYTPGVYPSQD 260 >ref|XP_007142345.1| hypothetical protein PHAVU_008G272700g [Phaseolus vulgaris] gi|561015478|gb|ESW14339.1| hypothetical protein PHAVU_008G272700g [Phaseolus vulgaris] Length = 478 Score = 152 bits (384), Expect = 1e-34 Identities = 72/107 (67%), Positives = 81/107 (75%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GDEDLS+PFS+ S+RYDLGTTE T +S G SDS+ SY N VNYQN P IHPAPKF Sbjct: 155 GPPAGDEDLSIPFSSSSDRYDLGTTETTVSSPGPASDSSPSYHNPVNYQNPPGIHPAPKF 214 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HDT N+QHS N PS+Q+HDRVD+NKH SYTP VYPSQD Sbjct: 215 HDTVNEQHSGNNQPSMQYHDRVDNNKHSSVVSPSSHSYTPGVYPSQD 261 >gb|KOM46565.1| hypothetical protein LR48_Vigan07g026900 [Vigna angularis] Length = 441 Score = 148 bits (374), Expect = 1e-33 Identities = 71/107 (66%), Positives = 83/107 (77%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GDEDLS+PFS+ S+ YD GTTE T ++ G ESDS+ SY N VNYQN P+IHPAPKF Sbjct: 119 GPPAGDEDLSIPFSSSSDGYDPGTTETTVSNPGPESDSSPSYHNPVNYQN-PNIHPAPKF 177 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HD+ N+QHSANIPPS+QFHD +D+NKH SYTP VYPSQD Sbjct: 178 HDSANEQHSANIPPSMQFHDGLDNNKHSSVVSPSSHSYTPGVYPSQD 224 >ref|XP_004491432.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5 [Cicer arietinum] Length = 462 Score = 114 bits (284), Expect = 4e-23 Identities = 60/108 (55%), Positives = 67/108 (62%), Gaps = 1/108 (0%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GDEDLSVP S+PSNRYD+GTTE + ++ S+SDSTHSY N VNYQN+PSIHPAPKF Sbjct: 155 GPPAGDEDLSVPASSPSNRYDIGTTETSASNAESDSDSTHSYHNPVNYQNMPSIHPAPKF 214 Query: 179 HDTP-NDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HDT ND S YTP VYPSQD Sbjct: 215 HDTTVNDTQS----------------------------YTPGVYPSQD 234 >ref|XP_002531202.1| Protein C6orf55, putative [Ricinus communis] gi|223529204|gb|EEF31179.1| Protein C6orf55, putative [Ricinus communis] Length = 422 Score = 99.0 bits (245), Expect = 1e-18 Identities = 52/107 (48%), Positives = 62/107 (57%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GDEDLS+P S P N YDLGTTE TS ESD + + N + SI P+ +F Sbjct: 152 GPPAGDEDLSIPSSAPGNGYDLGTTETVATSPRQESDQNSHFHDQFNDHHSTSIQPSVQF 211 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 H+T N+QHSA++ QFHD V DN H SYT A YP D Sbjct: 212 HETVNNQHSAHMASQPQFHDSV-DNHHPANIPPSHPSYTSAGYPPHD 257 >ref|XP_007020695.1| Hydroxyproline-rich glycoprotein family protein [Theobroma cacao] gi|508720323|gb|EOY12220.1| Hydroxyproline-rich glycoprotein family protein [Theobroma cacao] Length = 420 Score = 88.2 bits (217), Expect = 2e-15 Identities = 48/107 (44%), Positives = 58/107 (54%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP+GDEDLS+P STPS YDLG +E TS +SD P+P+F Sbjct: 155 GPPNGDEDLSIPSSTPSGAYDLGPSEPAVTSPRRDSD------------------PSPQF 196 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HD N+QH NIPPS FHD++ D +H SY A YPS D Sbjct: 197 HDEVNNQHYTNIPPSPHFHDKI-DRQHSSNISPTPPSYPSASYPSHD 242 >ref|XP_011013801.1| PREDICTED: vacuolar protein sorting-associated protein VTA1 homolog [Populus euphratica] Length = 427 Score = 81.3 bits (199), Expect = 3e-13 Identities = 43/107 (40%), Positives = 60/107 (56%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP DE+LS+P STPS YDLG +E+ ++ ESD + + + VN + +I P+P F Sbjct: 155 GPPADDENLSIPSSTPSGGYDLGPSESAASNARPESDPSPQFHDQVNDEPYTNIPPSPMF 214 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HD N+ HSA +PP F++ N+H Y A YPS D Sbjct: 215 HDNLNNHHSAQVPPPSLFYNSA-SNQH-LTDTPPSSFYPAAGYPSHD 259 >ref|XP_010096091.1| hypothetical protein L484_012446 [Morus notabilis] gi|587874103|gb|EXB63256.1| hypothetical protein L484_012446 [Morus notabilis] Length = 440 Score = 80.9 bits (198), Expect = 4e-13 Identities = 41/87 (47%), Positives = 52/87 (59%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GD+DLS+P S P YD +E+ + E D + YD SVN + +I P KF Sbjct: 155 GPPSGDDDLSIPSSNPDGAYDTAPSESASVNSRQEPDPSQFYD-SVNDKLSSNIPPPAKF 213 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKH 99 HD NDQHS NIPPS Q+ D+N+H Sbjct: 214 HDKVNDQHSTNIPPSFQYQ---DNNQH 237 >ref|XP_004300450.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5 [Fragaria vesca subsp. vesca] Length = 481 Score = 80.5 bits (197), Expect = 5e-13 Identities = 47/107 (43%), Positives = 63/107 (58%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GD+DLSVP T S+ YDLG +E TS G +SDS+ + + VN Q+ +I P+ ++ Sbjct: 155 GPPAGDDDLSVPSRTTSDSYDLGPSETAATSPGPQSDSSSQFYDEVNKQHPTNITPS-QY 213 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HD N Q SA+ S QF DR +D +H S+ A YPS D Sbjct: 214 HDKVNFQRSASFQSSPQFPDRFND-QHSADVLPSSQSFPSAGYPSHD 259 >ref|XP_006377238.1| hypothetical protein POPTR_0011s02470g [Populus trichocarpa] gi|550327432|gb|ERP55035.1| hypothetical protein POPTR_0011s02470g [Populus trichocarpa] Length = 436 Score = 77.4 bits (189), Expect = 4e-12 Identities = 45/116 (38%), Positives = 62/116 (53%), Gaps = 9/116 (7%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSES---------DSTHSYDNSVNYQNL 207 GPP DE+LS+P STPS YDLG +E+ ++ ES D + + + VN + Sbjct: 155 GPPADDENLSIPSSTPSGGYDLGPSESAASNARPESAASNARPESDPSPQFHDQVNDEPY 214 Query: 206 PSIHPAPKFHDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 +I P+P FHD N+ HSA++PP F+D N+H Y A YPSQD Sbjct: 215 TNIPPSPLFHDKVNNHHSAHVPPPSLFYDSA-SNQH-STDTPPPSFYPAAGYPSQD 268 >ref|XP_012461261.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5-like [Gossypium raimondii] gi|763746344|gb|KJB13783.1| hypothetical protein B456_002G093900 [Gossypium raimondii] Length = 420 Score = 77.0 bits (188), Expect = 5e-12 Identities = 45/104 (43%), Positives = 54/104 (51%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP+GDEDLSV ST S YD G E TS +SD P+P+F Sbjct: 155 GPPNGDEDLSVLSSTHSGSYDHGPGELPVTSPRQDSD------------------PSPQF 196 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYP 48 HD N+QH ANIPPS QFHD++ D +H S+ P YP Sbjct: 197 HDEINNQHHANIPPSTQFHDKI-DRQHSFDILASHPSFPPRSYP 239 >ref|XP_008235151.1| PREDICTED: vacuolar protein sorting-associated protein VTA1 homolog [Prunus mume] Length = 438 Score = 76.3 bits (186), Expect = 9e-12 Identities = 45/107 (42%), Positives = 59/107 (55%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP GD+DLSVP S S YDLG +E TS G +SD + + + VN Q+ P P+F Sbjct: 155 GPPAGDDDLSVPSSGMSGSYDLGPSEAAVTSPGPQSDPSPQFYDEVNKQHSTDTLP-PQF 213 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 HD Q SA++ S +FHD V + +H SY+ A Y S D Sbjct: 214 HDNAKIQRSASLQSSPEFHDEV-NGQHSSNNVPPPRSYSTAGYSSHD 259 >ref|XP_012070918.1| PREDICTED: protein HOMOLOG OF MAMMALIAN LYST-INTERACTING PROTEIN 5 [Jatropha curcas] gi|643732007|gb|KDP39199.1| hypothetical protein JCGZ_00956 [Jatropha curcas] Length = 425 Score = 74.3 bits (181), Expect = 3e-11 Identities = 44/107 (41%), Positives = 55/107 (51%) Frame = -2 Query: 359 GPPDGDEDLSVPFSTPSNRYDLGTTENTFTSGGSESDSTHSYDNSVNYQNLPSIHPAPKF 180 GPP DED S+P STP YDLG +E TS ESD + + VN QN SI P+ + Sbjct: 155 GPPADDEDFSIPSSTPGGGYDLGPSETAVTSPHPESDQLSRFYDQVNDQNSTSIPPSSQ- 213 Query: 179 HDTPNDQHSANIPPSVQFHDRVDDNKHXXXXXXXXXSYTPAVYPSQD 39 N+QH +++ QFHD +N H SY A YPS D Sbjct: 214 --KANNQHFSHLASQPQFHDSA-NNHHSTNIMPSPPSYPSAGYPSHD 257