BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Wisteria21_contig00009858
         (1450 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic [Cice...   483   e-133
ref|XP_013449701.1| isoamylase-type starch debranching enzyme [M...   468   e-129
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   447   e-122
ref|XP_007162398.1| hypothetical protein PHAVU_001G148700g [Phas...   432   e-118
gb|KHN19871.1| Isoamylase 2, chloroplastic [Glycine soja]             429   e-117
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ...   428   e-117
ref|XP_014496282.1| PREDICTED: isoamylase 2, chloroplastic [Vign...   420   e-114
gb|KOM38909.1| hypothetical protein LR48_Vigan03g229100 [Vigna a...   419   e-114
ref|XP_007149089.1| hypothetical protein PHAVU_005G040300g [Phas...   399   e-108
gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]                   393   e-106
ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prun...   334   1e-88
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun...   330   1e-87
ref|XP_009347699.1| PREDICTED: isoamylase 2, chloroplastic-like ...   327   1e-86
ref|XP_012092290.1| PREDICTED: isoamylase 2, chloroplastic [Jatr...   322   4e-85
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   322   5e-85
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic [Frag...   320   3e-84
ref|XP_010090367.1| Isoamylase 2 [Morus notabilis] gi|587849097|...   319   3e-84
ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50...   318   8e-84
ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like ...   318   1e-83
gb|KDO68805.1| hypothetical protein CISIN_1g047308mg [Citrus sin...   318   1e-83

>ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic [Cicer arietinum]
          Length = 858

 Score =  483 bits (1244), Expect = e-133
 Identities = 269/391 (68%), Positives = 297/391 (75%), Gaps = 13/391 (3%)
 Frame = -2

Query: 1140 MANLVLSSFSFSVIPRNHY-KYGTKLSFFHNSSIQRNXXXXXXL-------INPFSHSLT 985
            MANL L SFSFS+IP +H+  +GTK SFFH   IQ                I+PFSH+LT
Sbjct: 1    MANL-LPSFSFSLIPLDHHHNHGTKCSFFHTPFIQIKHQHQLVGKNLQDLIISPFSHNLT 59

Query: 984  SKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIE 805
            SKLCATSRLSVEETEQ+ + FTQSE +K ALSYLFRTEIG+GLV+VYV K+KV+Y VYIE
Sbjct: 60   SKLCATSRLSVEETEQKFSTFTQSEDIKTALSYLFRTEIGEGLVKVYVKKKKVTYFVYIE 119

Query: 804  VSSLEMELSGGE---LCWGVYRXXXXXXXXVGTGMNVSPLVQTSPGKFAVELEFDVKQVP 634
            VSSLE+  + GE   LCWGVYR         G GMNVSP VQ S GKF+VELEFDV+QVP
Sbjct: 120  VSSLELVRAEGETLVLCWGVYRDDSLSVNV-GKGMNVSPFVQNSLGKFSVELEFDVEQVP 178

Query: 633  XXXXXXXXXXXLEIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGVVL 454
                       LEIR+H KRNFCVPVG   GCP  LGLSFSPD S+NFAV+SR AE VVL
Sbjct: 179  FYLSFLLRYSGLEIRTHMKRNFCVPVGFYKGCPSLLGLSFSPDESVNFAVFSRRAESVVL 238

Query: 453  CLYDD--SGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSSYAA 280
            CLYDD    VEKPALEL+LDPYVNRSGDIWHISFES   FV YGYR   +++D  +SYA 
Sbjct: 239  CLYDDDNDSVEKPALELDLDPYVNRSGDIWHISFESVGNFVRYGYRLGGAHRD--NSYA- 295

Query: 279  ERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRLNVK 100
               VVLDPYARI+GNSFP NGIGSVKNLG L K+PAFDWGDD H NL MEKL+VYRLNVK
Sbjct: 296  -ECVVLDPYARIVGNSFP-NGIGSVKNLGFLKKDPAFDWGDDYHLNLDMEKLLVYRLNVK 353

Query: 99   RFTEHESSQLLGDLAGTFSGLAKKVQHFKDL 7
            RFTEHESSQL GDLAGTFSGLAKKVQHFKDL
Sbjct: 354  RFTEHESSQLPGDLAGTFSGLAKKVQHFKDL 384


>ref|XP_013449701.1| isoamylase-type starch debranching enzyme [Medicago truncatula]
            gi|657379349|gb|KEH23729.1| isoamylase-type starch
            debranching enzyme [Medicago truncatula]
          Length = 891

 Score =  468 bits (1203), Expect = e-129
 Identities = 261/391 (66%), Positives = 294/391 (75%), Gaps = 11/391 (2%)
 Frame = -2

Query: 1140 MANLVLSSFSFSVIPRNHYKYGTKLSFF----HNSSIQRNXXXXXXLINPFSHSLTSKLC 973
            MANL L SFSFS+IP NH+KY TK SF     HN  + +        I PFSH+ TSKL 
Sbjct: 1    MANL-LPSFSFSLIPLNHHKYETKSSFIQIKHHNQFLSKKIQDL---IIPFSHNHTSKLF 56

Query: 972  ATS-RLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSS 796
            ATS RLS+EETEQ+L PFTQS  +K + SYLFRTEIG+GLV+VYV K+K  + VYIEVSS
Sbjct: 57   ATSSRLSIEETEQKLIPFTQSVDIKTSFSYLFRTEIGEGLVKVYVKKKKDCFFVYIEVSS 116

Query: 795  LEMELSGGE---LCWGVYRXXXXXXXXVGTGMNVSPLVQTSPGKFAVELEFDVKQVPXXX 625
            LE+    GE   LCWG+YR         G GMNVSP V+ S GKF+VELEFDV+QVP   
Sbjct: 117  LELSNVKGETLVLCWGLYRDDSSSVDD-GKGMNVSPFVENSVGKFSVELEFDVEQVPLYL 175

Query: 624  XXXXXXXXLEIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGVVLCLY 445
                    LEIR+H KRNFCVPVG L G PDPLG+SFS DGS+NFAV+SRHAE VVLCLY
Sbjct: 176  SFLLRFSGLEIRTHLKRNFCVPVGFLRGRPDPLGISFSRDGSINFAVFSRHAESVVLCLY 235

Query: 444  ---DDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSSYAAER 274
               DDSG+E PALE++LDPYVNRSGDIWHI+FESA  FVSYGYRF  +N++  +SYA   
Sbjct: 236  DDDDDSGLENPALEIDLDPYVNRSGDIWHITFESARNFVSYGYRFRGANRN--NSYA--E 291

Query: 273  VVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRLNVKRF 94
             VVLDPYARI+GNSF  NGIGS KNLG L KEPAFDW DD HPNL MEKLVVYRLNVKRF
Sbjct: 292  GVVLDPYARIVGNSF-QNGIGSAKNLGFLRKEPAFDWSDDYHPNLEMEKLVVYRLNVKRF 350

Query: 93   TEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            TEHESSQL  DLAG FSGLAKK+QHFKDL +
Sbjct: 351  TEHESSQLSSDLAGKFSGLAKKLQHFKDLGV 381


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
            gi|734430146|gb|KHN45374.1| Isoamylase 2, chloroplastic
            [Glycine soja] gi|947045850|gb|KRG95479.1| hypothetical
            protein GLYMA_19G153700 [Glycine max]
          Length = 865

 Score =  447 bits (1150), Expect = e-122
 Identities = 247/396 (62%), Positives = 289/396 (72%), Gaps = 24/396 (6%)
 Frame = -2

Query: 1116 FSFSVIPRNHYKYGTKLSFFHNSSIQR----NXXXXXXLINPFSHSLTS-KLCATSRLSV 952
            FSFSVIPRNH+K   K SF H   IQR    +      L++PFS + TS KLCATSRLS+
Sbjct: 8    FSFSVIPRNHHKL--KRSFSHKPFIQRKQLWHQLGLQSLVSPFSLNPTSSKLCATSRLSI 65

Query: 951  EETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSGG 772
            EETEQQ+   T+ E LK +L+YLFRTE G GLV+V+VT+R   YSVYIE+SSL++    G
Sbjct: 66   EETEQQIGTLTRPEDLKGSLAYLFRTETGGGLVKVHVTRRNDRYSVYIEISSLDISGGVG 125

Query: 771  E---LCWGVYRXXXXXXXXVGT---------GMNVSPLVQTSPGKFAVELEFDVKQVPXX 628
            E   LCWGVYR        + T         GMNVSPLVQ S GKFA+ELEFD K VP  
Sbjct: 126  EALLLCWGVYRSDSSCFVDLDTIGLSENAAMGMNVSPLVQNSDGKFAIELEFDAKHVPLY 185

Query: 627  XXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGVV 457
                         EIRSHR+ NFC+PVGSLPG P PLG+S+SPDGS+NF+++SRHAE VV
Sbjct: 186  LSFFLMSSLDSGLEIRSHRRTNFCMPVGSLPGYPCPLGVSYSPDGSVNFSIFSRHAESVV 245

Query: 456  LCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYR----FLHSNKDDGSS 289
            LCLYD++GVEKPALEL+LDPYVNR+GDIWH+SFESA  FVSYGYR     L  NKDDG +
Sbjct: 246  LCLYDENGVEKPALELDLDPYVNRTGDIWHVSFESAKGFVSYGYRCRRGVLKKNKDDGFA 305

Query: 288  YAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRL 109
                  VVLDPYA+I+GNS+P +G+G VKNLG L KEP FDWG D HP+L+MEKLVVYRL
Sbjct: 306  ----EHVVLDPYAKIVGNSYP-DGVGLVKNLGCLRKEPFFDWGGDRHPDLSMEKLVVYRL 360

Query: 108  NVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            NVKRFT+HESSQL   LAGTF+GLAKKVQHFKDL +
Sbjct: 361  NVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGV 396


>ref|XP_007162398.1| hypothetical protein PHAVU_001G148700g [Phaseolus vulgaris]
            gi|139867055|dbj|BAF52942.1| isoamylase-type
            starch-debranching enzyme 2 [Phaseolus vulgaris]
            gi|561035862|gb|ESW34392.1| hypothetical protein
            PHAVU_001G148700g [Phaseolus vulgaris]
          Length = 865

 Score =  432 bits (1110), Expect = e-118
 Identities = 234/394 (59%), Positives = 280/394 (71%), Gaps = 18/394 (4%)
 Frame = -2

Query: 1128 VLSSFSFSVIPRNHYKYGTKLSFFHNSSIQRNXXXXXXLIN--PFSHSLTSKLCATSRLS 955
            V+S+FSFS+IP N +K+G K   FH   IQR         +  PF  + +SKLCATSRLS
Sbjct: 4    VVSAFSFSLIPFNQHKFGAKCVGFHKPLIQRKQRQLGLQNHFFPFFRNPSSKLCATSRLS 63

Query: 954  VEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSG 775
            +EETEQQ+ PFT+ E LK AL+YLFRTE G GLV+VYVTK+K  Y VYIE+SSL++   G
Sbjct: 64   IEETEQQVEPFTRPEDLKGALAYLFRTETGGGLVKVYVTKKKDRYFVYIEISSLDVNHCG 123

Query: 774  GE----LCWGVYRXXXXXXXXV-GTG--------MNVSPLVQTSPGKFAVELEFDVKQVP 634
                  LCWGVYR        +  TG        MNVSPLVQTS  KF VELEFD K VP
Sbjct: 124  DSETLVLCWGVYRGDSSCFVDMDSTGLSGNAAKRMNVSPLVQTSVCKFGVELEFDAKYVP 183

Query: 633  XXXXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEG 463
                           EI SHR+ NFCVPVG LPG P PLGLS+SPDGS+NFA++SRHAE 
Sbjct: 184  LYLSFFLMSSLDAGLEIISHRRTNFCVPVGLLPGYPGPLGLSYSPDGSVNFAIFSRHAES 243

Query: 462  VVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSSYA 283
            VVLCLYD+ GVEKPALE++LDPYVNRSGDIWH+SFES  +FVSYGYR         +  +
Sbjct: 244  VVLCLYDEKGVEKPALEVDLDPYVNRSGDIWHVSFESVKSFVSYGYRCRGGVHKQNNGDS 303

Query: 282  AERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRLNV 103
            +  +VVLDPYA+I+G+S+P +G+  V+NLG LGKEPAFDWG D  P+L+ME+LVVYRLNV
Sbjct: 304  SAELVVLDPYAKIVGHSYP-SGLELVQNLGWLGKEPAFDWGGDFLPDLSMEELVVYRLNV 362

Query: 102  KRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            KRFT+H SSQL    AGTF+GLA+KVQHFKDL +
Sbjct: 363  KRFTQHNSSQLPSGSAGTFTGLAEKVQHFKDLGV 396


>gb|KHN19871.1| Isoamylase 2, chloroplastic [Glycine soja]
          Length = 866

 Score =  429 bits (1104), Expect = e-117
 Identities = 242/396 (61%), Positives = 277/396 (69%), Gaps = 24/396 (6%)
 Frame = -2

Query: 1116 FSFSVIPRNHYKYGTKLSFFHNSSIQRNXXXXXXL----INPFSHSLTS-KLCATSRLSV 952
            FSFSVIP +H+    K S FH   IQR            I+PFS + TS KLCATSRLS+
Sbjct: 8    FSFSVIPGSHHSK-PKFSCFHEPFIQRKHLWPKLGLQNPISPFSRNPTSSKLCATSRLSI 66

Query: 951  EETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSGG 772
            EETEQQL   ++ E LK + +YLFRTE G GLV+VYVTKRK  Y VY E+SSL++  S G
Sbjct: 67   EETEQQLGTLSRPEYLKGSSAYLFRTETGGGLVKVYVTKRKDRYFVYTEISSLDISGSVG 126

Query: 771  E---LCWGVYRXXXXXXXXV---------GTGMNVSPLVQTSPGKFAVELEFDVKQVPXX 628
            E   LCWGVYR        +          TGMNVSPLVQ S G FAVELEFD K VP  
Sbjct: 127  ETLLLCWGVYRSDSSCFVDLDTIGLRENAATGMNVSPLVQNSDGNFAVELEFDAKHVPLY 186

Query: 627  XXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGVV 457
                         EIRSHR  NFCVPVG LPG P PLGL  SPDGS+NFA++SR AE VV
Sbjct: 187  LSFFLMSSLDAGMEIRSHRGTNFCVPVGLLPGYPGPLGLFCSPDGSVNFAIFSRRAESVV 246

Query: 456  LCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYR----FLHSNKDDGSS 289
            LCLYD++ +EKPALEL+LDPYVNR+GDIWH+SFESA  F+SYGY      L  NKDDG +
Sbjct: 247  LCLYDENDMEKPALELDLDPYVNRTGDIWHVSFESAKGFMSYGYSCRGGVLKRNKDDGFA 306

Query: 288  YAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRL 109
                  VVLDPYA+I+GNS+P +G+G VKNLG LGKEP FDWG DCH +L+MEKLVVYRL
Sbjct: 307  ----EHVVLDPYAKIVGNSYP-DGVGFVKNLGWLGKEPDFDWGGDCHLDLSMEKLVVYRL 361

Query: 108  NVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            NVKRFT+HESSQL   LAGTF+GLAKKVQHFKDL +
Sbjct: 362  NVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGV 397


>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
            gi|947118911|gb|KRH67160.1| hypothetical protein
            GLYMA_03G151200 [Glycine max]
          Length = 866

 Score =  428 bits (1101), Expect = e-117
 Identities = 241/396 (60%), Positives = 277/396 (69%), Gaps = 24/396 (6%)
 Frame = -2

Query: 1116 FSFSVIPRNHYKYGTKLSFFHNSSIQRNXXXXXXL----INPFSHSLTS-KLCATSRLSV 952
            FSFSVIP +H+    K S FH   IQR            I+PFS + TS KLCATSRLS+
Sbjct: 8    FSFSVIPGSHHSK-PKFSCFHEPFIQRKHLWPKLGLQNPISPFSRNPTSSKLCATSRLSI 66

Query: 951  EETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSGG 772
            EETEQQL   ++ E LK + +YLFRTE G GLV+VYVTKRK  Y VY E+SSL++  S G
Sbjct: 67   EETEQQLGTLSRPEYLKGSSAYLFRTETGGGLVKVYVTKRKDRYFVYTEISSLDISGSVG 126

Query: 771  E---LCWGVYRXXXXXXXXV---------GTGMNVSPLVQTSPGKFAVELEFDVKQVPXX 628
            E   LCWGVYR        +          TGMNVSPLVQ S G FAVELEFD K VP  
Sbjct: 127  ETLLLCWGVYRSDSSCFVDLDTIGLRENAATGMNVSPLVQNSDGNFAVELEFDAKHVPLY 186

Query: 627  XXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGVV 457
                         EIRSHR  NFCVPVG LPG P PLGL  SPDGS+NFA++SR AE VV
Sbjct: 187  LSFFLMSSLDAGMEIRSHRGTNFCVPVGLLPGYPGPLGLFCSPDGSVNFAIFSRRAESVV 246

Query: 456  LCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYR----FLHSNKDDGSS 289
            LCLYD++ +EKPALEL+LDPYVNR+GDIWH++FESA  F+SYGY      L  NKDDG +
Sbjct: 247  LCLYDENDMEKPALELDLDPYVNRTGDIWHVAFESAKGFMSYGYSCRGGVLKRNKDDGFA 306

Query: 288  YAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRL 109
                  VVLDPYA+I+GNS+P +G+G VKNLG LGKEP FDWG DCH +L+MEKLVVYRL
Sbjct: 307  ----EHVVLDPYAKIVGNSYP-DGVGFVKNLGWLGKEPDFDWGGDCHLDLSMEKLVVYRL 361

Query: 108  NVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            NVKRFT+HESSQL   LAGTF+GLAKKVQHFKDL +
Sbjct: 362  NVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGV 397


>ref|XP_014496282.1| PREDICTED: isoamylase 2, chloroplastic [Vigna radiata var. radiata]
          Length = 895

 Score =  420 bits (1079), Expect = e-114
 Identities = 230/393 (58%), Positives = 272/393 (69%), Gaps = 18/393 (4%)
 Frame = -2

Query: 1125 LSSFSFSVIPRNHYKYGTKLSFFHNSSIQ--RNXXXXXXLINPFSHSLTSKLCATSRLSV 952
            +S FS S+ P N +K+  K   FH   IQ   +      L  PF  + +SKLCATSRLS+
Sbjct: 35   MSLFSLSLTPFNQHKFEAKSFCFHKPLIQSKHHQLGSQNLFYPFFRNPSSKLCATSRLSI 94

Query: 951  EETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSGG 772
            EETEQQ+   T+ E LK ALSYLFRTE G GLV+VYVTK+K  Y VY+E+SSL++   G 
Sbjct: 95   EETEQQVGTLTRPEDLKGALSYLFRTETGGGLVKVYVTKKKDRYFVYVEISSLDVNHCGE 154

Query: 771  E----LCWGVYRXXXXXXXXV-GTG--------MNVSPLVQTSPGKFAVELEFDVKQVPX 631
                 LCWGVYR        +  TG        MNVSPLVQTS GKF VELEFD K VP 
Sbjct: 155  SETLVLCWGVYRGDASCFVDMDSTGLSGIAAKRMNVSPLVQTSVGKFGVELEFDAKYVPL 214

Query: 630  XXXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGV 460
                          EIRSHR+ NFCVPVG LPG P PLGLS+SPDGS+NFA++SRHAE V
Sbjct: 215  YLSFFLMSSLDAGLEIRSHRRTNFCVPVGLLPGYPSPLGLSYSPDGSVNFAIFSRHAESV 274

Query: 459  VLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSSYAA 280
            VLCLYD+ GVEKPALE++LDPYVNRSGD+WH+SFES  +FVSYG+R            ++
Sbjct: 275  VLCLYDEKGVEKPALEVDLDPYVNRSGDMWHVSFESVESFVSYGFRCRGGVHKQNRGDSS 334

Query: 279  ERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRLNVK 100
               VVLDPYA+I+ NS P  G+G V+NLG L KEPAFDWG D HP+L+ME+LVVYRLNVK
Sbjct: 335  AEHVVLDPYAKIVANSCP-GGLGLVQNLGWLRKEPAFDWGGDFHPDLSMEELVVYRLNVK 393

Query: 99   RFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            RFT+HESSQL    AGTF+GLA+KVQHFKDL +
Sbjct: 394  RFTQHESSQLPRGSAGTFTGLAEKVQHFKDLGV 426


>gb|KOM38909.1| hypothetical protein LR48_Vigan03g229100 [Vigna angularis]
          Length = 859

 Score =  419 bits (1078), Expect = e-114
 Identities = 228/393 (58%), Positives = 274/393 (69%), Gaps = 18/393 (4%)
 Frame = -2

Query: 1125 LSSFSFSVIPRNHYKYGTKLSFFHNSSIQR--NXXXXXXLINPFSHSLTSKLCATSRLSV 952
            +S FS S+IP N +K+  K   FH   IQR  +      L  PF  + +SKLCATSRLS+
Sbjct: 5    MSLFSLSLIPFNQHKFQAKSFCFHKPLIQRKHHQFGSQNLFFPFFRNPSSKLCATSRLSI 64

Query: 951  EETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSGG 772
            EETEQQ+   T+ E LK AL+YLFRTE G GLV+VYVTK+K  Y VY+E+SSL++   G 
Sbjct: 65   EETEQQVGTSTRPEDLKGALAYLFRTETGGGLVKVYVTKKKDRYFVYVEISSLDVNHCGE 124

Query: 771  E----LCWGVYRXXXXXXXXV-GTG--------MNVSPLVQTSPGKFAVELEFDVKQVPX 631
                 LCWGVYR        +  TG        MNVSPLVQTS GKF VELEFD K VP 
Sbjct: 125  SETLVLCWGVYRGDASCFVDMDSTGLSGIAAKRMNVSPLVQTSVGKFGVELEFDAKYVPL 184

Query: 630  XXXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEGV 460
                          EIRSHR+ NFCVPVG LPG P PLGLS+SPDGS+NF+++SRHAE V
Sbjct: 185  YLSFFLMSSLNAGLEIRSHRRTNFCVPVGLLPGYPSPLGLSYSPDGSVNFSIFSRHAESV 244

Query: 459  VLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSSYAA 280
            VLCLYD+ GVEKPALE++LDPYVNRSGD+WH+S E+  +FVSYG+R            ++
Sbjct: 245  VLCLYDEKGVEKPALEVDLDPYVNRSGDMWHVSLENVKSFVSYGFRCRGGVHKQNRGDSS 304

Query: 279  ERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRLNVK 100
               VVLDPYA+I+ NS P  G+G V+NLG L KEPAFDWGDD HP+L+ME+LVVYRLNVK
Sbjct: 305  AEHVVLDPYAKIVANSCP-GGLGLVQNLGWLRKEPAFDWGDDFHPDLSMEELVVYRLNVK 363

Query: 99   RFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            RFT+HESSQL    AGTF+GLA+KVQHFKD+ +
Sbjct: 364  RFTQHESSQLPSGSAGTFTGLAEKVQHFKDIGV 396


>ref|XP_007149089.1| hypothetical protein PHAVU_005G040300g [Phaseolus vulgaris]
            gi|561022353|gb|ESW21083.1| hypothetical protein
            PHAVU_005G040300g [Phaseolus vulgaris]
          Length = 853

 Score =  399 bits (1026), Expect = e-108
 Identities = 223/394 (56%), Positives = 270/394 (68%), Gaps = 18/394 (4%)
 Frame = -2

Query: 1128 VLSSFSFSVIPRNHYKYGTKLSFFHNSSIQR--NXXXXXXLINPFSHSLTSKLCATSRLS 955
            V+S FSFS+I  N +++G K   FH S IQR  +      L  PF  +  SKLCATS LS
Sbjct: 4    VVSGFSFSLISFNQHRFGAKCFGFHKSLIQRKHHQLGLQNLFFPFFRNPPSKLCATSLLS 63

Query: 954  VEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEVSSLEMELSG 775
            +EE+EQQ+   T+ E LK A+ YLFRTE G GLV+ YVT +K    VYIE+SSL +   G
Sbjct: 64   IEESEQQVGTVTRPEELKGAVPYLFRTETGGGLVKAYVTNKKDRCFVYIEISSLNVNHYG 123

Query: 774  GE----LCWGVYRXXXXXXXXV-GTGM--------NVSPLVQTSPGKFAVELEFDVKQVP 634
                  LCWGVYR        +  TG+        NVS LVQTS GKF VELEF+ K VP
Sbjct: 124  DSETLVLCWGVYRSYSFCFVDMDSTGLSGNLAKRVNVSRLVQTSVGKFGVELEFEAKYVP 183

Query: 633  XXXXXXXXXXXL---EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHAEG 463
                            I SHR+ NFCVPVG LPG P PLGLS+SPDGS+NFA++SRHAEG
Sbjct: 184  LYLSFFLKSSLNGGLAIISHRETNFCVPVGMLPGYPGPLGLSYSPDGSVNFAIFSRHAEG 243

Query: 462  VVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSSYA 283
            VVLCLYD+ GVEKPALE++LDP++NRSGD+WH+SFES  ++VSYGYR       +G S A
Sbjct: 244  VVLCLYDEKGVEKPALEVDLDPFMNRSGDMWHVSFESVKSYVSYGYR-CRGGVHNGDSSA 302

Query: 282  AERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRLNV 103
                VVLDPYA+I+G+S+P  G+G V+NLG L KEPAFDWG D HP+L ME+LVVYRLNV
Sbjct: 303  EH--VVLDPYAKIVGHSYP-GGLGLVQNLGWLRKEPAFDWGGDFHPDLPMEELVVYRLNV 359

Query: 102  KRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            KRFT+H+SSQL    AG F+GLA+KVQHFKDL +
Sbjct: 360  KRFTQHKSSQLPSGSAGIFTGLAEKVQHFKDLGV 393


>gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]
          Length = 857

 Score =  393 bits (1009), Expect = e-106
 Identities = 239/400 (59%), Positives = 271/400 (67%), Gaps = 20/400 (5%)
 Frame = -2

Query: 1140 MANLVLSSFSFSVIPRNHYKYGTKLSFFHNS--SIQRNXXXXXXLIN----PFSHSLTSK 979
            M NL L +FSFSV P   +KY TK SFFH S   IQ         +     PFS +LTSK
Sbjct: 1    MTNL-LPAFSFSVTP---HKYETKCSFFHTSFTCIQHQNQLVSKNLQDLKIPFSQNLTSK 56

Query: 978  LCATS-RLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEV 802
            LCATS RLS+EET Q+L  FTQSE +K AL YLFRTE G+GLV+VYV K+K +Y VYIEV
Sbjct: 57   LCATSSRLSIEETGQKLISFTQSEDIKTALPYLFRTETGEGLVKVYVKKKKDTYFVYIEV 116

Query: 801  SSLEMELSGGE---LCWGVYRXXXXXXXXVGTGMNVSPLVQTSPGKFAVELEFDVKQVPX 631
            SSLE+    GE   LCWGVY          G GMNVSPLV+ S GKF+VELEFDV+QVP 
Sbjct: 117  SSLELSNVEGETFVLCWGVYSDDSSSLGS-GKGMNVSPLVKNSLGKFSVELEFDVEQVPL 175

Query: 630  XXXXXXXXXXLEIRSHRKRNFCVPVGSLPGCPDPLGL-------SFSPDGSMNFAVYSRH 472
                       EI +H ++ F      L  C   LG+        F   G      +   
Sbjct: 176  YLSFLLRLSGSEITTHAEKKF------LRACLVFLGVVHLRWGSPFLLTGLSILQFFRGM 229

Query: 471  AEGVVLCLYD---DSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKD 301
             +GVVLCLYD   D+GV+KPALEL+LDPYVNRSGDIWHIS E+A +FVSY YRF  +N+D
Sbjct: 230  QQGVVLCLYDNNVDTGVKKPALELDLDPYVNRSGDIWHISLENAKSFVSYCYRFRGANRD 289

Query: 300  DGSSYAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLV 121
            +  SYA    VVLDPYARI+GNSFPN GIGSVKNLG L KEPAFDWGDD H NL MEKLV
Sbjct: 290  N--SYA--ECVVLDPYARIVGNSFPN-GIGSVKNLGFLRKEPAFDWGDDYHLNLDMEKLV 344

Query: 120  VYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            VYRLNVK FTEHESSQL GDLAGTFSGLAKK+QHFKDL +
Sbjct: 345  VYRLNVKHFTEHESSQLSGDLAGTFSGLAKKLQHFKDLGV 384


>ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prunus mume]
          Length = 883

 Score =  334 bits (857), Expect = 1e-88
 Identities = 184/352 (52%), Positives = 232/352 (65%), Gaps = 17/352 (4%)
 Frame = -2

Query: 1005 PFSHSLTSKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKV 826
            P  H+L  K+ A S++SVE  EQ+ +  T++E + +  +YLFRTEIGD +V V+V KR  
Sbjct: 64   PRDHNL--KVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGD-VVNVFVRKRNA 120

Query: 825  SYSVYIEVSSLEMELSGGELC--WGVYRXXXXXXXXVG---------TGMNVSPLVQTSP 679
             Y V IEV SL +  S   L   WG+YR        +          T    +P +QTS 
Sbjct: 121  KYIVNIEVPSLYLSSSDRRLVLHWGMYRADSSCFMPLDFKSSTPNDTTTTLETPFIQTSS 180

Query: 678  GKFAVELEFDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLS 517
            G+F +ELEF+ KQ+P                  EIRSHRK NFCVPVG   G P PLGL+
Sbjct: 181  GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 516  FSPDGSMNFAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFV 337
            FS DGSMNFA++SR+AE VVLCLYD++  EKPALEL+LDPYVNRSGDIWH+SF SAWTFV
Sbjct: 241  FSNDGSMNFAIFSRNAESVVLCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWTFV 300

Query: 336  SYGYRFLHSNKDDGSSYAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGD 157
            SYGY+F  +      +   E  V+LDPYA++I  S PNN    +K LGRL +EPAFDW  
Sbjct: 301  SYGYKFKRNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAG 360

Query: 156  DCHPNLAMEKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            D  P+L+MEKLVVYRLNV RFTEH+SSQL  ++ G+FSGL +K++HFKDL +
Sbjct: 361  DVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGV 412


>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
            gi|462397150|gb|EMJ02949.1| hypothetical protein
            PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score =  330 bits (847), Expect = 1e-87
 Identities = 181/352 (51%), Positives = 232/352 (65%), Gaps = 17/352 (4%)
 Frame = -2

Query: 1005 PFSHSLTSKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKV 826
            P  H+L  K+ A S++SVE  EQ+ +  T++E + +  +YLFRTEIGD +V V+V KR  
Sbjct: 64   PRDHNL--KVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGD-VVNVFVRKRNA 120

Query: 825  SYSVYIEVSSLEMELSGGELC--WGVYRXXXXXXXXVG---------TGMNVSPLVQTSP 679
             Y+V IEV S  +  +   L   WG+YR        +          T    +PL+QTS 
Sbjct: 121  KYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTSS 180

Query: 678  GKFAVELEFDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLS 517
            G+F +ELEF+ KQ+P                  EIRSHRK NFCVPVG   G P PLGL+
Sbjct: 181  GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 516  FSPDGSMNFAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFV 337
            FS DGSMNFA++SR+AE V LCLYD++  EKPALEL+LDPYVNRSGDIWH+SF SAW+FV
Sbjct: 241  FSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFV 300

Query: 336  SYGYRFLHSNKDDGSSYAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGD 157
            SYGY+F  +      +   E  V+LDPYA++I  S PNN    +K LGRL +EPAFDW  
Sbjct: 301  SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAG 360

Query: 156  DCHPNLAMEKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            D  P+L+MEKLVVYRLNV RFTEH+SSQL  ++ G+FSGL +K++HFKDL +
Sbjct: 361  DVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGV 412


>ref|XP_009347699.1| PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x bretschneideri]
          Length = 869

 Score =  327 bits (839), Expect = 1e-86
 Identities = 187/344 (54%), Positives = 225/344 (65%), Gaps = 17/344 (4%)
 Frame = -2

Query: 981  KLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEV 802
            K+ ATSR+SVE  EQ+++  T++E   +  +YLFRT+ GD LV V+V KR   Y+V IEV
Sbjct: 71   KVYATSRVSVEPMEQRVSTGTETEEPSKVSTYLFRTDTGD-LVNVFVRKRNAKYTVNIEV 129

Query: 801  SSLEMELSGGELC--WGVYRXXXXXXXXVG---------TGMNVSPLVQTSPGKFAVELE 655
            S L    S   L   WG+YR                   T    +P   TS GKF +ELE
Sbjct: 130  SLLHPSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFTGTSSGKFTLELE 189

Query: 654  FDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMN 493
            F+ KQ+P                  EIRSHRK NFC PVG   GCP PLGLSFS DGSMN
Sbjct: 190  FEAKQIPFYLSFVLKSPADADLSDLEIRSHRKTNFCFPVGFGRGCPAPLGLSFSNDGSMN 249

Query: 492  FAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLH 313
            FA++SR+AE VVLCLYD++  EKPALEL+LDPYVNRSGDIWH SFE AW FVSYGYRF  
Sbjct: 250  FAIFSRNAESVVLCLYDETTAEKPALELDLDPYVNRSGDIWHASFEKAWDFVSYGYRF-- 307

Query: 312  SNKDDGSSYAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAM 133
               D+G+       V+LDPYA+II  S P +G GS K LGRL +EPAFDW  D  PNL +
Sbjct: 308  ---DEGN-------VLLDPYAKIIAKSVP-HGTGS-KYLGRLREEPAFDWAGDVLPNLTL 355

Query: 132  EKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            EKLVVYRLNVKRFTEH+SS+L  ++AGTFSGL +K++HFK L +
Sbjct: 356  EKLVVYRLNVKRFTEHKSSKLPTNIAGTFSGLTQKLEHFKALGV 399


>ref|XP_012092290.1| PREDICTED: isoamylase 2, chloroplastic [Jatropha curcas]
            gi|643704433|gb|KDP21497.1| hypothetical protein
            JCGZ_21968 [Jatropha curcas]
          Length = 865

 Score =  322 bits (826), Expect = 4e-85
 Identities = 179/347 (51%), Positives = 232/347 (66%), Gaps = 16/347 (4%)
 Frame = -2

Query: 993  SLTSKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSV 814
            +L S+  A +R+ +++TE+  +  T+ +  K+  +YLFR++IG G V+V+V K+   ++V
Sbjct: 66   TLHSRAFAAARVPIQQTERMFSTSTEVDEWKKVSTYLFRSQIG-GHVKVFVRKKNGKHAV 124

Query: 813  YIEVSSLEMELSGGELC--WGVYRXXXXXXXXVGTG-------MNVSPLVQTSPGKFAVE 661
            YIEVSSL++     +L   WG+YR        + +           +P VQ S G+FA+E
Sbjct: 125  YIEVSSLDISTRDYKLTLIWGIYRSDSACFMPLDSQHLDPDARTMDTPFVQNSFGRFALE 184

Query: 660  LEFDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGS 499
            LEF+ KQ P                  EIR+HR+ NFCVP+G   G P+PLGLSFS DGS
Sbjct: 185  LEFEAKQTPIYLSFLLKSMFNSDSSSLEIRNHRQTNFCVPIGFSSGYPNPLGLSFSNDGS 244

Query: 498  MNFAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRF 319
            MNFA +SR+AEGVVLCLYDDS  +KPALEL+LDPYVNRSGD+WH S ESAWTF SYGYR 
Sbjct: 245  MNFAFFSRNAEGVVLCLYDDSTTDKPALELDLDPYVNRSGDVWHASLESAWTFTSYGYRC 304

Query: 318  LHSNKDDGSSYAAERVVVLDPYARIIGNSFPNNGIG-SVKNLGRLGKEPAFDWGDDCHPN 142
              + K D      ER V+LDPYARII N   ++G G S K LGRL + PAF+WGDD  PN
Sbjct: 305  KGTGKVD-----VER-VLLDPYARIIVNCTADDGSGLSTKYLGRLCEVPAFEWGDDVRPN 358

Query: 141  LAMEKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            LAMEKLVVYRLNVK FTE +SS+L  D+ GTF+GL +K+ HFK+L +
Sbjct: 359  LAMEKLVVYRLNVKHFTESKSSKLYSDIDGTFAGLTEKLNHFKNLGV 405


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  322 bits (825), Expect = 5e-85
 Identities = 173/348 (49%), Positives = 227/348 (65%), Gaps = 17/348 (4%)
 Frame = -2

Query: 993  SLTSKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSV 814
            SL S++C+ +R+ V++TE + +     + L +  +YLFRT+ G G V+V V K+   Y+V
Sbjct: 57   SLDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFG-GHVKVLVRKKNAKYAV 115

Query: 813  YIEVSSLEMELSGGELC--WGVYRXXXXXXXXVGTGMNVSP--------LVQTSPGKFAV 664
            YIEVSSLE+  +   L   WG+YR        + +  N +P        LVQ S G FA+
Sbjct: 116  YIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDS-QNFAPNARKMDTALVQNSFGTFAL 174

Query: 663  ELEFDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDG 502
            ELEF+ KQ P                  EI++H+  NFCVP+G   G P PLGLSFS DG
Sbjct: 175  ELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFSTDG 234

Query: 501  SMNFAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYR 322
            SMNFA +SR+ EG+VLCLYDDS  +KPALEL+LDPYVNR+GD+WH S E AWTF SYGYR
Sbjct: 235  SMNFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYGYR 294

Query: 321  FLHSNKDDGSSYAAERVVVLDPYARIIGNSFPNNGIG-SVKNLGRLGKEPAFDWGDDCHP 145
               +     +S      V+LDPYAR+I +S  ++G   S K LGRL +EPAF+WG D  P
Sbjct: 295  CKGAILQGNTSKVDMECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEWGSDIRP 354

Query: 144  NLAMEKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            NLAMEKL+VYRLNVKRFTEH+S +L  D+AGTF+GL +K+ HF++L +
Sbjct: 355  NLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGV 402


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic [Fragaria vesca subsp. vesca]
          Length = 868

 Score =  320 bits (819), Expect = 3e-84
 Identities = 175/336 (52%), Positives = 224/336 (66%), Gaps = 8/336 (2%)
 Frame = -2

Query: 984  SKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIE 805
            S++ A S +S+E  EQ     T +E   +  +YLFRTE GD L+ V+V    V+YSVY+E
Sbjct: 67   SRVQAASGVSIEPMEQNFP--TGTEETNKVSTYLFRTENGD-LINVFVRDNTVNYSVYVE 123

Query: 804  VSSLEMELSGGELC--WGVYRXXXXXXXXVGTGMNVSPLVQTSPGKFAVELEFDVKQVPX 631
            +SSL++  +G  L   WG+YR        +      +P  +TS G F +ELEF+ KQ P 
Sbjct: 124  LSSLQLSSAGDRLVISWGMYRADSSSLKPLD--FIETPFTKTSSGSFTLELEFEAKQTPF 181

Query: 630  XXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAVYSRHA 469
                             EIRSHRK NFCVPVG   GCP PLGLS+S DGS+NFA++SR+A
Sbjct: 182  YLSFTVKSLADANLSGLEIRSHRKTNFCVPVGFGRGCPTPLGLSYSSDGSINFAIFSRNA 241

Query: 468  EGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNKDDGSS 289
            E VVLCLYDD   ++PALEL+LDPYVNRSGDIWH SFESAWTF+SYGYRF  ++  + +S
Sbjct: 242  ESVVLCLYDDPTAQEPALELDLDPYVNRSGDIWHASFESAWTFLSYGYRFKGTSLRNTNS 301

Query: 288  YAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAMEKLVVYRL 109
               E  V+LDPYA++I  S  NN    +K LGRL +EP FDW  D  P L +EKLVVYRL
Sbjct: 302  -LDEGNVLLDPYAKVIDESIANNRGTGLKLLGRLCEEPVFDWDGDVRPLLPIEKLVVYRL 360

Query: 108  NVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            N+KRFTEH+SS+L  ++AGTFSGL +K+ HF+DL +
Sbjct: 361  NLKRFTEHKSSKLPTNVAGTFSGLTQKLDHFQDLGV 396


>ref|XP_010090367.1| Isoamylase 2 [Morus notabilis] gi|587849097|gb|EXB39337.1| Isoamylase
            2 [Morus notabilis]
          Length = 825

 Score =  319 bits (818), Expect = 3e-84
 Identities = 176/347 (50%), Positives = 231/347 (66%), Gaps = 16/347 (4%)
 Frame = -2

Query: 993  SLTSKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSV 814
            +L+ ++ ATSR+S+E  +++L+  T+ E  ++  +YLFRTE+G GLV V+V K+KV+ +V
Sbjct: 18   NLSVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVG-GLVNVFVRKKKVNCTV 76

Query: 813  YIEVSSLEMELSGGEL--CWGVYRXXXXXXXXVGTGMNV---SPLVQTSPGKFAVELEFD 649
            Y+EVSSLE+  S G L   WG+YR             +    +P  + S G++ VE EF+
Sbjct: 77   YVEVSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETSTPVETPFTKNSFGRYTVEFEFE 136

Query: 648  VKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFA 487
             K++P                  +IRSHRK NFCVP+G   G P PLGLSFS DGS+NF+
Sbjct: 137  AKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLGLSFSRDGSLNFS 196

Query: 486  VYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRF---- 319
            ++SR AE VVLCLYDD+  + PALEL+LDPYVNR+GDIWH SFES+ TFVSYGYR     
Sbjct: 197  IFSRSAESVVLCLYDDTTADNPALELDLDPYVNRTGDIWHASFESSSTFVSYGYRLKGSR 256

Query: 318  LHSNKDDGSSYAAERVVVLDPYARIIGNS-FPNNGIGSVKNLGRLGKEPAFDWGDDCHPN 142
            L   KD+ +       +VLDPYA+IIG S   ++GIG  K LGR+ KEP FDW  D  PN
Sbjct: 257  LKGKKDEDAR------IVLDPYAKIIGKSTSSDHGIGP-KYLGRISKEPGFDWSGDIRPN 309

Query: 141  LAMEKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            L +EKL VYRLNV  FTEH+SSQL  D+AGTFSGL +K++HF DL +
Sbjct: 310  LPIEKLTVYRLNVMHFTEHKSSQLSPDIAGTFSGLIEKLEHFTDLGV 356


>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
            Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  318 bits (815), Expect = 8e-84
 Identities = 186/343 (54%), Positives = 229/343 (66%), Gaps = 12/343 (3%)
 Frame = -2

Query: 993  SLTSKLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSV 814
            +L  +  A SR+SVE+TEQ  T  TQ + LK+  +YLFRTEIG G V+V+V KR V++ V
Sbjct: 66   NLDLRCFAASRVSVEQTEQIFTS-TQVDELKKLSTYLFRTEIG-GQVKVFVRKRSVNHVV 123

Query: 813  YIEVSSLEMELSGGE----LCWGVYRXXXXXXXXVGTGMNVSPLVQTSPGKFAVELEFDV 646
             IEVSSL+  LSG E    L  G++R            +  +P +  S  + A+ELEF+ 
Sbjct: 124  DIEVSSLQ--LSGDESQLVLSGGIHRSDHDIKNR----IIETPFIAKSSSELALELEFEA 177

Query: 645  KQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMNFAV 484
            K+ P                  EIR+HRK NFCVPVG   G P PLGLSFS DGSMNFAV
Sbjct: 178  KEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLGLSFSTDGSMNFAV 237

Query: 483  YSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLHSNK 304
            YSR+AE +VLCLYDD+  EKPALEL+LDP+VNR+GDIWH S E +WTFV YGYR     K
Sbjct: 238  YSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTFVRYGYRC----K 293

Query: 303  DDGSSYAAERVVVLDPYARIIGNSFPNNGIGS--VKNLGRLGKEPAFDWGDDCHPNLAME 130
             D  ++ AER V+LDPYARIIG+S PN+      +K+LGRL KEPAFDW  D  PNL ME
Sbjct: 294  GDTDAFNAER-VLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSSDVCPNLPME 352

Query: 129  KLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            KLVVYRLNV  FTE +SS+L  D+ GTF+G+ +KVQH KDL +
Sbjct: 353  KLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGV 395


>ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
          Length = 870

 Score =  318 bits (814), Expect = 1e-83
 Identities = 180/344 (52%), Positives = 225/344 (65%), Gaps = 17/344 (4%)
 Frame = -2

Query: 981  KLCATSRLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIEV 802
            K+ ATSR+SVE  EQ+++  T++E + +  +YLFRT+ GD  V V+V KR   Y+V IEV
Sbjct: 71   KVYATSRVSVEPVEQRVSTGTETEEVGKVSTYLFRTDTGDP-VNVFVRKRNAKYAVNIEV 129

Query: 801  SSLEMELSGGELC--WGVYRXXXXXXXXVG---------TGMNVSPLVQTSPGKFAVELE 655
            S L +  S   L   WG+YR                   T    +P   TS GKF +ELE
Sbjct: 130  SLLHLSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFTGTSSGKFTLELE 189

Query: 654  FDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSMN 493
            F+ KQ+P                  EIRSHRK NFC PVG   GCP PLG+SFS DGSMN
Sbjct: 190  FEAKQIPFYLSFVLKSPADADLSDLEIRSHRKTNFCFPVGFGRGCPAPLGVSFSNDGSMN 249

Query: 492  FAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFLH 313
            FA++SR+AE VVLCLY ++  EKP LEL+LDPYVNRSGDIWH SFE+AW FVSYGYRF  
Sbjct: 250  FAIFSRNAESVVLCLYAETTAEKPVLELDLDPYVNRSGDIWHASFENAWDFVSYGYRF-- 307

Query: 312  SNKDDGSSYAAERVVVLDPYARIIGNSFPNNGIGSVKNLGRLGKEPAFDWGDDCHPNLAM 133
               D+G+       V+LDPYA+II  S P +G GS K LGRL +EPAF+W  D  P+L +
Sbjct: 308  ---DEGN-------VLLDPYAKIIAKSVP-HGTGS-KYLGRLCEEPAFEWAGDVXPDLTL 355

Query: 132  EKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            EKLVVYRLNVKRFT+H+SS+L  ++AGTFSGL +K++HFK L +
Sbjct: 356  EKLVVYRLNVKRFTKHKSSKLPTNIAGTFSGLTEKLEHFKALGV 399


>gb|KDO68805.1| hypothetical protein CISIN_1g047308mg [Citrus sinensis]
          Length = 878

 Score =  318 bits (814), Expect = 1e-83
 Identities = 181/348 (52%), Positives = 228/348 (65%), Gaps = 21/348 (6%)
 Frame = -2

Query: 981  KLCATS-RLSVEETEQQLTPFTQSEALKRALSYLFRTEIGDGLVRVYVTKRKVSYSVYIE 805
            + CA+S R+SV+ET+   TP T+ + L++  +YLFRT++G GLV V V ++ V+Y+V I+
Sbjct: 73   RACASSSRVSVDETQPVFTPTTEVDELQKVSTYLFRTDLG-GLVSVSVGEKNVNYAVTID 131

Query: 804  VSSLEMELSGGELC--WGVYRXXXXXXXXVG---------TGMNVSPLVQTSPGKFAVEL 658
            VSSL+    G +L   WGVY         +G         TG   +P   TS   FA+EL
Sbjct: 132  VSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTFALEL 191

Query: 657  EFDVKQVPXXXXXXXXXXXL------EIRSHRKRNFCVPVGSLPGCPDPLGLSFSPDGSM 496
             F+ KQ P                  EIR+H    FCVPVG   G P P+GLSFS DGS+
Sbjct: 192  GFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPMGLSFSTDGSL 251

Query: 495  NFAVYSRHAEGVVLCLYDDSGVEKPALELELDPYVNRSGDIWHISFESAWTFVSYGYRFL 316
            NFA++SRHA+GVVLCLYDD+  ++PALEL+LDPY+NRSGDIWH S ES W FVSYGYRF 
Sbjct: 252  NFAIFSRHAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMESTWNFVSYGYRFK 311

Query: 315  HS-NKDDGSSYAAERVVVLDPYARIIGNSFPN-NGIG-SVKNLGRLGKEPAFDWGDDCHP 145
             S ++ DG     E  V+LDPYA+II NS PN + +G   K LGRL KEP FDWG D H 
Sbjct: 312  GSFSQGDGYKSHLES-VLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFDWGGDVHL 370

Query: 144  NLAMEKLVVYRLNVKRFTEHESSQLLGDLAGTFSGLAKKVQHFKDLEI 1
            NL MEKLVVYRLNV RF+EH+SS+L  D+AGTFSG+ +KV H KDL +
Sbjct: 371  NLPMEKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGV 418