BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Wisteria21_contig00005480
         (707 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago ...   376   e-102
sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase;...   376   e-102
gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]    366   7e-99
ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   362   2e-97
gb|AFK43950.1| unknown [Medicago truncatula]                          360   5e-97
ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   357   5e-96
emb|CAB56742.1| cytochrome P450 monooxygenase [Cicer arietinum]       355   1e-95
gb|AAO31712.1| cytochrome P450 [Pisum sativum]                        327   6e-87
ref|XP_003611658.1| cytochrome P450 family 81 protein [Medicago ...   314   3e-83
ref|XP_010095333.1| Isoflavone 2'-hydroxylase [Morus notabilis] ...   313   9e-83
ref|XP_007206553.1| hypothetical protein PRUPE_ppa019965mg [Prun...   313   9e-83
ref|XP_009372454.1| PREDICTED: isoflavone 2'-hydroxylase-like [P...   312   1e-82
ref|XP_004287985.1| PREDICTED: cytochrome P450 81E8-like [Fragar...   312   2e-82
gb|KOM25512.1| hypothetical protein LR48_Vigan107s003100 [Vigna ...   311   3e-82
gb|KHN13402.1| Isoflavone 2'-hydroxylase [Glycine soja] gi|94705...   310   4e-82
ref|XP_003548043.2| PREDICTED: isoflavone 2'-hydroxylase [Glycin...   310   4e-82
ref|XP_004509096.1| PREDICTED: cytochrome P450 81E8-like [Cicer ...   310   4e-82
sp|Q6WNQ8.1|C81E8_MEDTR RecName: Full=Cytochrome P450 81E8 [Medi...   310   6e-82
ref|XP_008238660.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   308   2e-81
ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   308   2e-81

>ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago truncatula]
           gi|657397628|gb|KEH37344.1| cytochrome P450 family 81
           protein [Medicago truncatula]
          Length = 503

 Score =  376 bits (965), Expect = e-102
 Identities = 190/235 (80%), Positives = 206/235 (87%), Gaps = 1/235 (0%)
 Frame = -3

Query: 705 DEHRNGN-HSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAE 529
           +EHR GN HS G TMIDHLLKLSESQPEYYS+ +IKGLI  MLLAGTDTSAVTIEWVM+E
Sbjct: 263 EEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSE 322

Query: 528 LLNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSE 349
           LLNHPEVLKKAKEELDTQIGK+KLVDEQDLSKLPYLQNIISETLR          H SSE
Sbjct: 323 LLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSE 382

Query: 348 DCTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACP 169
           DCTIG F VP+DTI+LTNVW IHRDP  W+ A SFKPERFEKE EVNK++AFGLGRRACP
Sbjct: 383 DCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEVNKVMAFGLGRRACP 442

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPI 4
           GL+LAQRTVGFT+GLLIQ FEW+RESEEKLDMMEGKGITMP + P++AMCKALPI
Sbjct: 443 GLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALPI 497


>sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase; AltName: Full=Cytochrome
           P450 81E9, partial [Medicago truncatula]
           gi|33521519|gb|AAQ20041.1| isoflavone 3'-hydroxylase,
           partial [Medicago truncatula]
          Length = 501

 Score =  376 bits (965), Expect = e-102
 Identities = 190/235 (80%), Positives = 206/235 (87%), Gaps = 1/235 (0%)
 Frame = -3

Query: 705 DEHRNGN-HSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAE 529
           +EHR GN HS G TMIDHLLKLSESQPEYYS+ +IKGLI  MLLAGTDTSAVTIEWVM+E
Sbjct: 261 EEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSE 320

Query: 528 LLNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSE 349
           LLNHPEVLKKAKEELDTQIGK+KLVDEQDLSKLPYLQNIISETLR          H SSE
Sbjct: 321 LLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSE 380

Query: 348 DCTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACP 169
           DCTIG F VP+DTI+LTNVW IHRDP  W+ A SFKPERFEKE EVNK++AFGLGRRACP
Sbjct: 381 DCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEVNKVMAFGLGRRACP 440

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPI 4
           GL+LAQRTVGFT+GLLIQ FEW+RESEEKLDMMEGKGITMP + P++AMCKALPI
Sbjct: 441 GLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALPI 495


>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  366 bits (940), Expect = 7e-99
 Identities = 178/237 (75%), Positives = 206/237 (86%), Gaps = 2/237 (0%)
 Frame = -3

Query: 705 DEHRNGNHSG--GDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMA 532
           ++H+N NHS   GDTMIDHLLKL E+QPEYYS+ MIKGLI AMLLAGTDTSAVTIEWVMA
Sbjct: 264 EQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMA 323

Query: 531 ELLNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSS 352
           ELLN+PEVLKKAK+E++T IGK++LV+EQDLS+LPYLQN+ISE LR          H +S
Sbjct: 324 ELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRLHPPAPLLLPHSAS 383

Query: 351 EDCTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRAC 172
           EDCTIGGF VP+DTI+LTN+WAIHRDP +W    SFKPERFEKEGEVNKL++FGLGRRAC
Sbjct: 384 EDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKEGEVNKLLSFGLGRRAC 443

Query: 171 PGLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           PGL+LAQRTVG+T+GLLIQGFEWK ESEEKLD+ EGKGITMP + P++AMCK LPIV
Sbjct: 444 PGLSLAQRTVGYTVGLLIQGFEWKTESEEKLDLAEGKGITMPMKFPLRAMCKPLPIV 500


>ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  362 bits (928), Expect = 2e-97
 Identities = 177/235 (75%), Positives = 203/235 (86%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           +E ++GN + GDTMIDHLLKL ESQPEYYS+ MIKGLI AMLLAGTDTSAV IEWVM+EL
Sbjct: 261 EEIQSGNQNDGDTMIDHLLKLRESQPEYYSDHMIKGLIQAMLLAGTDTSAVAIEWVMSEL 320

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHP+VL KAKEE++TQIG+DKL++E DLSKLPYLQNII+ETLR          H SSED
Sbjct: 321 LNHPDVLNKAKEEIETQIGRDKLIEEHDLSKLPYLQNIINETLRLHPPAPLLLPHYSSED 380

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
            T+GGF VP+DTI+L+NVWAIHRDPN+W    SFKPERFE EGE+NKLIAFGLGRRACPG
Sbjct: 381 FTLGGFNVPKDTIILSNVWAIHRDPNLWSDPLSFKPERFEIEGELNKLIAFGLGRRACPG 440

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           LNLAQR+VG T+G+LIQ FEWKRES+EKLDMME KGITMPK+ P++AMCK LPIV
Sbjct: 441 LNLAQRSVGLTVGMLIQCFEWKRESDEKLDMMEDKGITMPKKVPLEAMCKPLPIV 495


>gb|AFK43950.1| unknown [Medicago truncatula]
          Length = 227

 Score =  360 bits (924), Expect = 5e-97
 Identities = 181/221 (81%), Positives = 196/221 (88%)
 Frame = -3

Query: 666 MIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAELLNHPEVLKKAKEE 487
           MIDHLLKLSESQPEYYS+ +IKGLI  MLLAGTDTSAVTIEWVM+ELLNHPEVLKKAKEE
Sbjct: 1   MIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEE 60

Query: 486 LDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSEDCTIGGFKVPRDTI 307
           LDTQIGK+KLVDEQDLSKLPYLQNIISETLR          H SSEDCTIG F VP+DTI
Sbjct: 61  LDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTI 120

Query: 306 LLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPGLNLAQRTVGFTLG 127
           +LTNVW IHRDP  W+ A SFKPERFEKE EVNK++AFGLGRRACPGL+LAQRTVGFT+G
Sbjct: 121 ILTNVWGIHRDPKHWNDALSFKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVG 180

Query: 126 LLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPI 4
           LLIQ FEW+RESEEKLDMMEGKGITMP + P++AMCKALPI
Sbjct: 181 LLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALPI 221


>ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  357 bits (915), Expect = 5e-96
 Identities = 174/234 (74%), Positives = 198/234 (84%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           +EH++GN S GDTMIDHLL L +SQPEYYS+ MIKGLI AMLLAGTDTSAVTIEWVM+EL
Sbjct: 261 EEHQSGNQSNGDTMIDHLLNLRKSQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMSEL 320

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHP+VL KAKEE++TQIGKDKL++E DLSKLPYLQNII+ETLR          H SSED
Sbjct: 321 LNHPDVLNKAKEEIETQIGKDKLIEEHDLSKLPYLQNIINETLRLHPPAPLSLPHYSSED 380

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
            T+GGF VP+DTI+LTNVWAIHRDP  W  A SFKPERFE +GE+NKLIAFGLGRRACPG
Sbjct: 381 FTLGGFNVPKDTIILTNVWAIHRDPKYWSDASSFKPERFEIDGELNKLIAFGLGRRACPG 440

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPI 4
            +LA RTVG TLGLLIQ FEWKRE+EEK+D+ EGKG+TMP   P++AMCK LPI
Sbjct: 441 QSLAHRTVGLTLGLLIQCFEWKRETEEKIDLREGKGLTMPMGVPLKAMCKPLPI 494


>emb|CAB56742.1| cytochrome P450 monooxygenase [Cicer arietinum]
          Length = 240

 Score =  355 bits (912), Expect = 1e-95
 Identities = 175/232 (75%), Positives = 198/232 (85%)
 Frame = -3

Query: 696 RNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAELLNH 517
           R GN + GDTMIDHLLKL  +QPEYYS+ MIKGLI AMLLAGTDTSAV IEWVM+ELLNH
Sbjct: 4   RGGNQNDGDTMIDHLLKLRVTQPEYYSDHMIKGLIQAMLLAGTDTSAVAIEWVMSELLNH 63

Query: 516 PEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSEDCTI 337
           P+VL KAKEE++TQIG+DKL +E DLSKLPYLQNII+ETLR          H SSED T+
Sbjct: 64  PDVLNKAKEEIETQIGRDKLKEEHDLSKLPYLQNIINETLRLHPPAPLLLPHYSSEDFTL 123

Query: 336 GGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPGLNL 157
           GGF VP+DTI+L+NVWAIHRDPN+W    SFKPERFE EGE+NKLIAFGLGRRACPGLNL
Sbjct: 124 GGFNVPKDTIILSNVWAIHRDPNLWSDPLSFKPERFEIEGELNKLIAFGLGRRACPGLNL 183

Query: 156 AQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           AQR+VG T+G+LIQ FEWKRES+EKLDMME KGITMPK+ P++AMCK LPIV
Sbjct: 184 AQRSVGLTVGMLIQCFEWKRESDEKLDMMEDKGITMPKKVPLEAMCKPLPIV 235


>gb|AAO31712.1| cytochrome P450 [Pisum sativum]
          Length = 502

 Score =  327 bits (837), Expect = 6e-87
 Identities = 164/235 (69%), Positives = 186/235 (79%), Gaps = 1/235 (0%)
 Frame = -3

Query: 702 EHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAELL 523
           EHR+ NH  GDTMIDHLLKLSE Q       +IKGLI AMLLAGTDTSA+ IEWVM+ELL
Sbjct: 262 EHRSANHGNGDTMIDHLLKLSEVQLNIIPSHIIKGLIQAMLLAGTDTSALAIEWVMSELL 321

Query: 522 NHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSEDC 343
           NHPEVL KAKEE++TQ+GK+K++DEQDL KLPYLQNIISE L+          H SSEDC
Sbjct: 322 NHPEVLNKAKEEIETQVGKNKIIDEQDLPKLPYLQNIISEALKLHPPAPLLLPHYSSEDC 381

Query: 342 TIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPGL 163
           TIGGF VP+DTI+LTNVWAIHRDP  W  A SFKPERFEKEGEVNKL+AFG+GRRACPGL
Sbjct: 382 TIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGEVNKLMAFGIGRRACPGL 441

Query: 162 NLAQRTVGFTLGLL-IQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           +LAQRTVG   G    Q FEWKRESEEKLDMMEGK +   KR+P +A+CK   ++
Sbjct: 442 SLAQRTVGLNCGACWFQCFEWKRESEEKLDMMEGKVLPCLKRSPFKALCKGTTLL 496


>ref|XP_003611658.1| cytochrome P450 family 81 protein [Medicago truncatula]
           gi|355512993|gb|AES94616.1| cytochrome P450 family 81
           protein [Medicago truncatula]
          Length = 499

 Score =  314 bits (805), Expect = 3e-83
 Identities = 152/230 (66%), Positives = 183/230 (79%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEHR G  +  +TMIDHLL   +SQPEYY++Q+IKGL++ MLLAGTDTS+VTIEW M+ L
Sbjct: 260 DEHRFGKRNS-NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNL 318

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE++KKAK ELDT IG D+ VDE D+SKLPYLQ+I+ ETLR          H SSED
Sbjct: 319 LNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSED 378

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
            ++GG+ +P++TIL+ N W IHRDPN+W     FKPERFEKEGEVNKL++FGLGRRACPG
Sbjct: 379 FSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGEVNKLLSFGLGRRACPG 438

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCK 16
            NLAQRTVG TLGLLIQ FEWKR  EEK+DM+E KGIT+ K+T + AMCK
Sbjct: 439 ENLAQRTVGLTLGLLIQCFEWKRIGEEKIDMVEAKGITVGKKTSLNAMCK 488


>ref|XP_010095333.1| Isoflavone 2'-hydroxylase [Morus notabilis]
           gi|587870254|gb|EXB59544.1| Isoflavone 2'-hydroxylase
           [Morus notabilis]
          Length = 507

 Score =  313 bits (801), Expect = 9e-83
 Identities = 156/236 (66%), Positives = 182/236 (77%), Gaps = 1/236 (0%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           +EHR+   S  +TMIDHLL L ESQPEYY++Q+IKG IL +L AGT TSAVT+EW M+ L
Sbjct: 262 EEHRSRKESR-NTMIDHLLSLQESQPEYYTDQIIKGFILVLLFAGTHTSAVTLEWAMSNL 320

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHP +LKKAK E+D ++G + LVDEQDLSKL YLQNIISETLR          H SS D
Sbjct: 321 LNHPHILKKAKAEIDAEVGPENLVDEQDLSKLQYLQNIISETLRLYPAAPLLVPHYSSHD 380

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFE-KEGEVNKLIAFGLGRRACP 169
           CTIGG+ VPRDTILL N WAI RDP +WD A SFKPERFE  EG+ NK++ FGLGRRACP
Sbjct: 381 CTIGGYDVPRDTILLVNAWAIQRDPKLWDDATSFKPERFEISEGDTNKMMPFGLGRRACP 440

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           G  LAQR VG TLG LIQ FEWKR +EE++DM EG+G TMPK  P++AMCKA PI+
Sbjct: 441 GAGLAQRVVGQTLGSLIQCFEWKRVNEEEVDMTEGRGFTMPKAMPLEAMCKARPIM 496


>ref|XP_007206553.1| hypothetical protein PRUPE_ppa019965mg [Prunus persica]
           gi|462402195|gb|EMJ07752.1| hypothetical protein
           PRUPE_ppa019965mg [Prunus persica]
          Length = 499

 Score =  313 bits (801), Expect = 9e-83
 Identities = 155/236 (65%), Positives = 187/236 (79%), Gaps = 1/236 (0%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEH++   +G  TMIDHLL L ESQPEYY+ Q+IKGLIL MLLAGTDTSAVT+EW M+ L
Sbjct: 260 DEHKSKGKNG-TTMIDHLLSLQESQPEYYTNQIIKGLILVMLLAGTDTSAVTLEWAMSNL 318

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LN+P VLKKA+ ELD Q+G++ LVDE DLSKLPYLQNIISETLR          H SS+D
Sbjct: 319 LNNPHVLKKARVELDAQLGEENLVDEPDLSKLPYLQNIISETLRLCPAAPLLVPHFSSDD 378

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEV-NKLIAFGLGRRACP 169
           CTIGGF VPRDT++L N WA+HRDP +WD  +SF PERFE  G++ +KLI FGLGRRACP
Sbjct: 379 CTIGGFDVPRDTMILINAWALHRDPQLWDDPESFMPERFESGGDLSHKLIPFGLGRRACP 438

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           GL LAQR VG TLG LIQ FEW+R ++E++DM EGKG+TMPK  P++AMC+A  ++
Sbjct: 439 GLGLAQRVVGLTLGSLIQCFEWERITKEEIDMAEGKGLTMPKVVPLEAMCRARSVM 494


>ref|XP_009372454.1| PREDICTED: isoflavone 2'-hydroxylase-like [Pyrus x bretschneideri]
          Length = 499

 Score =  312 bits (800), Expect = 1e-82
 Identities = 154/236 (65%), Positives = 182/236 (77%), Gaps = 1/236 (0%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEHR+   +GG +MIDHLL L ESQPEYY++Q+IKGLI+ MLLAGTDTSAVT+EW M+ L
Sbjct: 260 DEHRSKGRNGG-SMIDHLLSLQESQPEYYTDQIIKGLIMVMLLAGTDTSAVTLEWAMSNL 318

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHP VL+KA  ELD ++G++ LVDE D+SKLPYLQ+IISETLR          H SS+D
Sbjct: 319 LNHPRVLQKANSELDAKVGRENLVDEPDISKLPYLQSIISETLRLCPAAPMLVPHLSSDD 378

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEK-EGEVNKLIAFGLGRRACP 169
           C IGGF VPRDT++L N WA+HRDP +WD  +SFKPERFE  E   +KLI FG+GRRACP
Sbjct: 379 CIIGGFDVPRDTMVLVNAWAVHRDPELWDEPESFKPERFESGEDFSHKLIPFGMGRRACP 438

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           G  LAQR VGFTLG LIQ F+W R  EE +DM EGKG T PK  P++AMCKA PIV
Sbjct: 439 GAGLAQRVVGFTLGSLIQCFDWNRVGEEMVDMTEGKGTTTPKAIPLEAMCKARPIV 494


>ref|XP_004287985.1| PREDICTED: cytochrome P450 81E8-like [Fragaria vesca subsp. vesca]
          Length = 499

 Score =  312 bits (799), Expect = 2e-82
 Identities = 154/236 (65%), Positives = 183/236 (77%), Gaps = 1/236 (0%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEHR    +   TMIDHLL L ESQPEYY++Q+IKGLI+ +LLAGTDTSAVT+EW MA L
Sbjct: 262 DEHRGTKRN---TMIDHLLSLQESQPEYYTDQIIKGLIVVLLLAGTDTSAVTLEWAMANL 318

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHP VLKKA+ ELD+ +G D+LVDE D+ KLPYLQ+I+SETLR          H SS+D
Sbjct: 319 LNHPHVLKKARTELDSLLGPDRLVDEPDVPKLPYLQSIVSETLRLYPAAPLLVPHLSSDD 378

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEK-EGEVNKLIAFGLGRRACP 169
           C IGGF VPRDT++L N WAIHRDP +W+  +SFKPERFE+ EGE  KL+ FGLGRRACP
Sbjct: 379 CVIGGFDVPRDTMVLINAWAIHRDPEVWEDPESFKPERFERGEGESQKLMPFGLGRRACP 438

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           G  LAQR +G TLG LIQ FEW+R  E ++DM EGKG+TMPK  P++AMCKA PIV
Sbjct: 439 GAGLAQRVLGLTLGSLIQCFEWERVDETEIDMTEGKGLTMPKAVPLEAMCKAHPIV 494


>gb|KOM25512.1| hypothetical protein LR48_Vigan107s003100 [Vigna angularis]
          Length = 500

 Score =  311 bits (797), Expect = 3e-82
 Identities = 150/234 (64%), Positives = 181/234 (77%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           +EHR+G H   +TMI+HLL + E+QPEYYS+ +IKGLI  MLLAGTDT+AVT+EW MA L
Sbjct: 258 EEHRSGKHKT-NTMIEHLLTMQETQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAMASL 316

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE+LKKA++E+D  +G+D+LVDE D+  LPY+QNII ETLR          H SSE 
Sbjct: 317 LNHPEILKKAQDEIDKCVGQDRLVDESDIPNLPYIQNIIYETLRLFSPAPMLLPHYSSEQ 376

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
           CT+GGF VPRDTI+L N WAI RDP  W  A  FKPERF +EGE NKLI FGLGRRACPG
Sbjct: 377 CTLGGFTVPRDTIVLINAWAIQRDPQTWSDATCFKPERFGQEGEANKLIPFGLGRRACPG 436

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPI 4
           + LA R++G TLGLLIQ FEWKR S+E++DM E KG+ MPK  P++AMCKA PI
Sbjct: 437 IGLAHRSMGLTLGLLIQCFEWKRPSDEEIDMRENKGVAMPKLIPLEAMCKARPI 490


>gb|KHN13402.1| Isoflavone 2'-hydroxylase [Glycine soja]
           gi|947059027|gb|KRH08433.1| hypothetical protein
           GLYMA_16G149300 [Glycine max]
          Length = 498

 Score =  310 bits (795), Expect = 4e-82
 Identities = 148/230 (64%), Positives = 180/230 (78%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           D+HRNG H   +TMIDHLL   +SQPEYY++Q+IKGL L MLLAGTDTSAVT+EW M+ L
Sbjct: 257 DQHRNGKHRA-NTMIDHLLAQQQSQPEYYTDQIIKGLALVMLLAGTDTSAVTLEWAMSNL 315

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE+LKKAK ELDT IG+D+LVDE D+ KLPYLQ+I+ ETLR          H SSED
Sbjct: 316 LNHPEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHPAAPMLVPHLSSED 375

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
           CTIG + +P++TILL N WAIHRDP +W     FKPERFE E E NKL+ FGLGRRACPG
Sbjct: 376 CTIGEYNIPQNTILLVNAWAIHRDPKLWSDPTHFKPERFENESEANKLLPFGLGRRACPG 435

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCK 16
            NLAQRT+  TL LLIQ FEWKR +++++DM EGKG+T+ K+ P++AMC+
Sbjct: 436 ANLAQRTLSLTLALLIQCFEWKRTTKKEIDMTEGKGLTVSKKYPLEAMCQ 485


>ref|XP_003548043.2| PREDICTED: isoflavone 2'-hydroxylase [Glycine max]
          Length = 519

 Score =  310 bits (795), Expect = 4e-82
 Identities = 148/230 (64%), Positives = 180/230 (78%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           D+HRNG H   +TMIDHLL   +SQPEYY++Q+IKGL L MLLAGTDTSAVT+EW M+ L
Sbjct: 278 DQHRNGKHRA-NTMIDHLLAQQQSQPEYYTDQIIKGLALVMLLAGTDTSAVTLEWAMSNL 336

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE+LKKAK ELDT IG+D+LVDE D+ KLPYLQ+I+ ETLR          H SSED
Sbjct: 337 LNHPEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHPAAPMLVPHLSSED 396

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
           CTIG + +P++TILL N WAIHRDP +W     FKPERFE E E NKL+ FGLGRRACPG
Sbjct: 397 CTIGEYNIPQNTILLVNAWAIHRDPKLWSDPTHFKPERFENESEANKLLPFGLGRRACPG 456

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCK 16
            NLAQRT+  TL LLIQ FEWKR +++++DM EGKG+T+ K+ P++AMC+
Sbjct: 457 ANLAQRTLSLTLALLIQCFEWKRTTKKEIDMTEGKGLTVSKKYPLEAMCQ 506


>ref|XP_004509096.1| PREDICTED: cytochrome P450 81E8-like [Cicer arietinum]
          Length = 506

 Score =  310 bits (795), Expect = 4e-82
 Identities = 150/231 (64%), Positives = 178/231 (77%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEHR      G+TMIDHLLKL ESQPEYY++Q+IKG++L MLL GT+TSA T+EW M+ L
Sbjct: 257 DEHRR-KKKNGNTMIDHLLKLQESQPEYYTDQIIKGIVLVMLLGGTETSATTLEWAMSAL 315

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE+L KA+EE+DT IG D+LV+E D+S LPYLQNII+ET R          H SS+D
Sbjct: 316 LNHPEILNKAREEIDTNIGLDRLVEESDISNLPYLQNIINETFRLHPAFALLAPHFSSKD 375

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
           CTIGG+ VP+ TILL N WAIHRD  +W+    FKPERFEKEGE +KLI FGLGRRACPG
Sbjct: 376 CTIGGYNVPQGTILLVNAWAIHRDSKLWNDPTQFKPERFEKEGETDKLIPFGLGRRACPG 435

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKA 13
            +L QRTVG TL L IQ FEWKR SE+++DM EGKG T PK  P++AMCKA
Sbjct: 436 ASLGQRTVGLTLALFIQCFEWKRISEKEIDMTEGKGATTPKLIPLEAMCKA 486


>sp|Q6WNQ8.1|C81E8_MEDTR RecName: Full=Cytochrome P450 81E8 [Medicago truncatula]
           gi|33521521|gb|AAQ20042.1| CYP81E8 [Medicago truncatula]
          Length = 499

 Score =  310 bits (794), Expect = 6e-82
 Identities = 150/230 (65%), Positives = 181/230 (78%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEHR G  +  +TMIDHLL   +SQPEYY++Q+IKGL++ MLLAGTDTS+VTIEW M+ L
Sbjct: 260 DEHRFGKRNS-NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNL 318

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE++KKAK ELDT IG D+ VDE D+SKLPYLQ+I+ ETLR          H SSED
Sbjct: 319 LNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSED 378

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
            ++GG+ +P++TIL+ N W IHRDPN+W     FKPERFEKEGEVNKL++FGLGRRACPG
Sbjct: 379 FSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGEVNKLLSFGLGRRACPG 438

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCK 16
            NL+QRT G TLGLLIQ FEWKR  EEK+DM+E KGIT  K+T + AMCK
Sbjct: 439 ENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNAMCK 488


>ref|XP_008238660.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Prunus mume]
           gi|645220087|ref|XP_008238667.1| PREDICTED: isoflavone
           2'-hydroxylase-like isoform X1 [Prunus mume]
           gi|645220089|ref|XP_008238676.1| PREDICTED: isoflavone
           2'-hydroxylase-like isoform X2 [Prunus mume]
          Length = 499

 Score =  308 bits (790), Expect = 2e-81
 Identities = 152/236 (64%), Positives = 186/236 (78%), Gaps = 1/236 (0%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           DEH++   +G  TMIDHLL L ESQPEYY+ Q+IKGLIL MLLAGTDTSAVT+EW M+ L
Sbjct: 260 DEHKSKGKNG-TTMIDHLLSLQESQPEYYTNQIIKGLILVMLLAGTDTSAVTLEWAMSNL 318

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LN+P VL+KA+ ELD Q+G++ LVDE DLSKLPYLQ+IISETLR          H SS+D
Sbjct: 319 LNNPHVLEKARVELDAQLGQENLVDEPDLSKLPYLQSIISETLRLCPAAPLLVPHLSSDD 378

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEV-NKLIAFGLGRRACP 169
           CTIGGF VPRDT++L N WA+HRDP +WD  +SF PERFE  G++ +KLI FGLGRRACP
Sbjct: 379 CTIGGFDVPRDTMILINAWALHRDPQLWDDPESFMPERFESGGDLSHKLIPFGLGRRACP 438

Query: 168 GLNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPIV 1
           G+ LAQR VG TLG LIQ FEW+R  +E++DM EGKG+TMPK  P++AMC+A  ++
Sbjct: 439 GIGLAQRVVGLTLGSLIQCFEWERIGKEEIDMAEGKGLTMPKVVPLEAMCRARSVM 494


>ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
           gi|734395408|gb|KHN28918.1| Isoflavone 2'-hydroxylase
           [Glycine soja] gi|947093845|gb|KRH42430.1| hypothetical
           protein GLYMA_08G089400 [Glycine max]
          Length = 500

 Score =  308 bits (789), Expect = 2e-81
 Identities = 148/234 (63%), Positives = 180/234 (76%)
 Frame = -3

Query: 705 DEHRNGNHSGGDTMIDHLLKLSESQPEYYSEQMIKGLILAMLLAGTDTSAVTIEWVMAEL 526
           +EHR+G H   +TMI+HLL + ESQP YYS+ +IKGLI  MLLAGTDT+AV IEW ++ L
Sbjct: 255 EEHRSGKHKA-NTMIEHLLTMQESQPHYYSDHIIKGLIQGMLLAGTDTTAVAIEWAVSSL 313

Query: 525 LNHPEVLKKAKEELDTQIGKDKLVDEQDLSKLPYLQNIISETLRXXXXXXXXXXHCSSED 346
           LNHPE+LKKAK+E+D  +G+D+LVDE D+ KLPYLQNII ETLR          H SSE+
Sbjct: 314 LNHPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFAPAPLLLPHYSSEE 373

Query: 345 CTIGGFKVPRDTILLTNVWAIHRDPNIWDVADSFKPERFEKEGEVNKLIAFGLGRRACPG 166
           CTIGGF +PRDTI+L N WAI RDP  W  A  FKPERFE+EGE NKLI FGLGRRACPG
Sbjct: 374 CTIGGFTIPRDTIVLINAWAIQRDPEHWSDATCFKPERFEQEGEANKLIPFGLGRRACPG 433

Query: 165 LNLAQRTVGFTLGLLIQGFEWKRESEEKLDMMEGKGITMPKRTPVQAMCKALPI 4
           + LA R++G TLGLLIQ FEWKR ++E++DM E KG+ +PK  P++AM K  PI
Sbjct: 434 IGLAHRSMGLTLGLLIQCFEWKRPTDEEIDMRENKGLALPKLIPLEAMFKTRPI 487