BLASTX nr result
ID: Wisteria21_contig00000871
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Wisteria21_contig00000871
(672 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus] 317 4e-84
ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago ... 308 1e-81
sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase;... 308 1e-81
ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [C... 301 2e-79
gb|AAO31712.1| cytochrome P450 [Pisum sativum] 301 2e-79
ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [C... 295 2e-77
gb|ABD17289.1| isoflavone 3'-hydroxylase, partial [Astragalus mo... 266 6e-69
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G... 261 3e-67
ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 260 5e-67
gb|AFC88269.1| isoflavone 3'-hydroxylase 1, partial [Astragalus ... 260 5e-67
ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 259 1e-66
dbj|BAT21113.1| cytochrome P450 [synthetic construct] 258 2e-66
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;... 258 2e-66
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata] 257 5e-66
ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum] ... 257 5e-66
ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arieti... 256 7e-66
dbj|BAT21114.1| cytochrome P450 [synthetic construct] 254 3e-65
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus] 254 3e-65
ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago ... 254 3e-65
ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phas... 251 2e-64
>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
Length = 505
Score = 317 bits (812), Expect = 4e-84
Identities = 154/187 (82%), Positives = 169/187 (90%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
ILTVKI+LQ QSRRLKNLPPGPPT+PIIGNLHHLKHPLHRTF TLSQKYGDIISLWFGSR
Sbjct: 15 ILTVKIILQIQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQKYGDIISLWFGSR 74
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
LV+V++SPS+ QECF+KNDV+ ANRP+FLTGK+IFYNYTTLGSA YGDHWRNLRRITTID
Sbjct: 75 LVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTID 134
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLSNHRLNSF G+R+DET RLV KL+NDVVK+ +F VELR RLTEMTFNAMMR
Sbjct: 135 VLSNHRLNSFHGVRKDETLRLVEKLRNDVVKE----GNFSFTDVELRHRLTEMTFNAMMR 190
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 191 MISGKRY 197
>ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago truncatula]
gi|657397628|gb|KEH37344.1| cytochrome P450 family 81
protein [Medicago truncatula]
Length = 503
Score = 308 bits (790), Expect = 1e-81
Identities = 150/187 (80%), Positives = 163/187 (87%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
I+T+KILL+ SRRLKNLPPGPPT+PIIGNLHHLKHPLHRTF TLSQ YGDI SLWFGSR
Sbjct: 15 IITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSR 74
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
LV+V+SSPSL ECF+KND+I ANRPRFLTGK+IFYNYTTLGSA YGDHWRNLRRITTID
Sbjct: 75 LVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTID 134
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLSN+RLNSFLG+RRDET RL+ KL DVV + F KVELR RLTEMTFNAMMR
Sbjct: 135 VLSNNRLNSFLGVRRDETNRLIQKLLKDVVSE-----GFGFTKVELRPRLTEMTFNAMMR 189
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 190 MISGKRY 196
>sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase; AltName: Full=Cytochrome
P450 81E9, partial [Medicago truncatula]
gi|33521519|gb|AAQ20041.1| isoflavone 3'-hydroxylase,
partial [Medicago truncatula]
Length = 501
Score = 308 bits (790), Expect = 1e-81
Identities = 150/187 (80%), Positives = 163/187 (87%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
I+T+KILL+ SRRLKNLPPGPPT+PIIGNLHHLKHPLHRTF TLSQ YGDI SLWFGSR
Sbjct: 13 IITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSR 72
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
LV+V+SSPSL ECF+KND+I ANRPRFLTGK+IFYNYTTLGSA YGDHWRNLRRITTID
Sbjct: 73 LVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTID 132
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLSN+RLNSFLG+RRDET RL+ KL DVV + F KVELR RLTEMTFNAMMR
Sbjct: 133 VLSNNRLNSFLGVRRDETNRLIQKLLKDVVSE-----GFGFTKVELRPRLTEMTFNAMMR 187
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 188 MISGKRY 194
>ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
Length = 500
Score = 301 bits (772), Expect = 2e-79
Identities = 144/187 (77%), Positives = 163/187 (87%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
I+T+KI+LQ QSR+LKNLPPGPPT+PIIGNLHHLKHP+HRTF TLSQKYGD++SLWFGSR
Sbjct: 15 IITIKIILQIQSRKLKNLPPGPPTIPIIGNLHHLKHPIHRTFTTLSQKYGDVLSLWFGSR 74
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
LV+V+SSPSLV ECF+KND+I ANRP+FLTGK+IF NYTTLGSA YGDHWRNLRRITTID
Sbjct: 75 LVVVVSSPSLVNECFTKNDIILANRPQFLTGKYIFNNYTTLGSASYGDHWRNLRRITTID 134
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLSN+RLNSFLG+R DET RLV KL + + + F KVELR RLTEMTFN MMR
Sbjct: 135 VLSNNRLNSFLGVRTDETNRLVQKLLKNTISE-------GFTKVELRHRLTEMTFNGMMR 187
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 188 MISGKRY 194
>gb|AAO31712.1| cytochrome P450 [Pisum sativum]
Length = 502
Score = 301 bits (771), Expect = 2e-79
Identities = 147/187 (78%), Positives = 162/187 (86%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
I+T+KILLQTQSRRLKNLPPGPPT+PIIGNLHHLKHPLHRTFATLSQKYGDI+SLWFGSR
Sbjct: 15 IITIKILLQTQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFATLSQKYGDIVSLWFGSR 74
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
LV+VISS SLV+ECF KNDV+ ANRPRFLTGK+IFY+YTTLGS YGDHWRNLRRITTID
Sbjct: 75 LVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLRRITTID 134
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLSN+RL SFLG+R DE RL+ + + GA+ F KVELRS LTEMTFN MMR
Sbjct: 135 VLSNNRLTSFLGVRTDEANRLIQNI-------IKHGASRDFIKVELRSILTEMTFNGMMR 187
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 188 MISGKRY 194
>ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
Length = 500
Score = 295 bits (754), Expect = 2e-77
Identities = 139/187 (74%), Positives = 160/187 (85%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
I+T+KI++Q QSR+LKNLPPGPPT+PIIGNLH LKHP+H TF TLSQKYGD++SLWFGSR
Sbjct: 15 IITIKIIIQIQSRKLKNLPPGPPTIPIIGNLHQLKHPMHHTFTTLSQKYGDVLSLWFGSR 74
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
LV+V+SSPSLVQECF+KND++ ANRP+ L GK+IFYNYTTLGS+ YGDHWRNLRRIT ID
Sbjct: 75 LVVVVSSPSLVQECFNKNDIVLANRPQLLAGKYIFYNYTTLGSSSYGDHWRNLRRITAID 134
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLSN+RLNSFLG+R DET RL+ KL ND V + F KVELR RLTEMTFN MM
Sbjct: 135 VLSNNRLNSFLGVRTDETNRLIQKLLNDTV------SDQGFTKVELRHRLTEMTFNGMMM 188
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 189 MISGKRY 195
>gb|ABD17289.1| isoflavone 3'-hydroxylase, partial [Astragalus mongholicus]
Length = 268
Score = 266 bits (681), Expect = 6e-69
Identities = 129/159 (81%), Positives = 142/159 (89%)
Frame = -2
Query: 479 GNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSLVQECFSKNDVIFANRPRF 300
GNLHHLKHPLHRTF TLSQKYGDIISLWFGSRLV+V++SPS+ QECF+KNDV+ ANRP+F
Sbjct: 1 GNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKF 60
Query: 299 LTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSFLGIRRDETKRLVHKLQND 120
LTGK+IFYNYTTLGSA YGDHWRNLRRITTIDVLSNHRLNSF G+R+DET RLV KL+ND
Sbjct: 61 LTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRND 120
Query: 119 VVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
VVK+ +F VELR RLTEMTFNAMMRMISGKRY
Sbjct: 121 VVKE----GNFSFTDVELRHRLTEMTFNAMMRMISGKRY 155
>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
gi|947093846|gb|KRH42431.1| hypothetical protein
GLYMA_08G089500 [Glycine max]
Length = 502
Score = 261 bits (667), Expect = 3e-67
Identities = 127/186 (68%), Positives = 153/186 (82%)
Frame = -2
Query: 560 LTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRL 381
L +K+LLQ +R+ +NLPPGPP++PIIGNLHHLK PLHRTF LS KYG +ISLWFGSRL
Sbjct: 18 LLLKLLLQ--ARKFQNLPPGPPSLPIIGNLHHLKRPLHRTFRALSDKYGHVISLWFGSRL 75
Query: 380 VMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDV 201
V+V+SS +L QECF+KNDV+ ANRPRFL+GKHIFYNYTTLGS+PYG+HWRNLRRIT +DV
Sbjct: 76 VVVVSSQTLFQECFTKNDVVLANRPRFLSGKHIFYNYTTLGSSPYGEHWRNLRRITALDV 135
Query: 200 LSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRM 21
LS HRL+SF IRRDET RLV KL + ++ +FA+VEL S+ +MTFN +MRM
Sbjct: 136 LSTHRLHSFAAIRRDETHRLVRKL----AEAQGSESSLSFAEVELTSKFYDMTFNNIMRM 191
Query: 20 ISGKRY 3
ISGKRY
Sbjct: 192 ISGKRY 197
>ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
Length = 511
Score = 260 bits (665), Expect = 5e-67
Identities = 124/182 (68%), Positives = 153/182 (84%)
Frame = -2
Query: 548 ILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVI 369
I L QSRR KNLPPGPP++PIIGNLHHLK PLHRTF L++KYGD+ISLWFGSRLV+V+
Sbjct: 20 IRLLFQSRRFKNLPPGPPSLPIIGNLHHLKRPLHRTFKELTKKYGDVISLWFGSRLVVVV 79
Query: 368 SSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNH 189
SS S Q+CF+KNDV+ ANRP+F++GK+IFYNYTTLGSA YG+HWRNLRRIT++DVLSNH
Sbjct: 80 SSLSEFQQCFTKNDVVLANRPKFISGKYIFYNYTTLGSASYGEHWRNLRRITSLDVLSNH 139
Query: 188 RLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGK 9
R+N+F GIR+DET+RL +KK+ E +++ FA+VEL R +MTFN +MRMISGK
Sbjct: 140 RINNFSGIRKDETQRL--------IKKLAEDSSSNFAEVELSFRFYDMTFNNIMRMISGK 191
Query: 8 RY 3
RY
Sbjct: 192 RY 193
>gb|AFC88269.1| isoflavone 3'-hydroxylase 1, partial [Astragalus membranaceus]
Length = 262
Score = 260 bits (665), Expect = 5e-67
Identities = 126/156 (80%), Positives = 139/156 (89%)
Frame = -2
Query: 470 HHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSLVQECFSKNDVIFANRPRFLTG 291
HHLKHPLHRTF TLSQKYGDIISLWFGSRLV+V++SPS+ QECF+KNDV+ ANRP+FLTG
Sbjct: 1 HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG 60
Query: 290 KHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSFLGIRRDETKRLVHKLQNDVVK 111
K+IFYNYTTLGSA YGDHWRNLRRITTIDVLSNHRLNSF G+R+DET RLV KL+NDVVK
Sbjct: 61 KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK 120
Query: 110 KVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
+ +F VELR RLTEMTFNAMMRMISGKRY
Sbjct: 121 E----GNFSFTDVELRHRLTEMTFNAMMRMISGKRY 152
>ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
Length = 498
Score = 259 bits (661), Expect = 1e-66
Identities = 125/182 (68%), Positives = 150/182 (82%)
Frame = -2
Query: 548 ILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVI 369
I L QSR+ KNLPPGPP++PIIGNLHHLK PLHRTF LS+ YGDIISLWFGSRLV+V+
Sbjct: 20 IRLLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVV 79
Query: 368 SSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNH 189
SS S Q+CF+KNDV+ ANRPRFL+GK+IFYNYTTLGS Y +HWRNLRRIT++DVLSNH
Sbjct: 80 SSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNH 139
Query: 188 RLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGK 9
R+N+F G+RRDET+RL+ KL D ++T+FA+VEL R +MTFN +MRMISGK
Sbjct: 140 RINNFSGVRRDETQRLITKLAED--------SSTSFAEVELSFRFYDMTFNNIMRMISGK 191
Query: 8 RY 3
RY
Sbjct: 192 RY 193
>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
Length = 477
Score = 258 bits (660), Expect = 2e-66
Identities = 122/177 (68%), Positives = 149/177 (84%)
Frame = -2
Query: 533 QSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSL 354
++R+ KNLPPGPP++PIIGNLHHLK PLHRTF LS+KYG + SLWFGSRLV+V+SS S
Sbjct: 3 RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASE 62
Query: 353 VQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSF 174
Q+CF+KNDV+ ANRPRFL+GK+IFYNYTTLGS YG+HWRNLRRIT +DVLSNHR+NSF
Sbjct: 63 FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 122
Query: 173 LGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
GIRRDET+RL+ +L +D ++T FA++EL SRL +MTFN +MRMISGKRY
Sbjct: 123 SGIRRDETQRLITRLADD--------SSTNFAEMELSSRLYDMTFNNIMRMISGKRY 171
>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
AltName: Full=Cytochrome P450 81E1; AltName:
Full=Cytochrome P450 91A4 [Glycyrrhiza echinata]
gi|2443348|dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza
echinata]
Length = 499
Score = 258 bits (660), Expect = 2e-66
Identities = 122/177 (68%), Positives = 149/177 (84%)
Frame = -2
Query: 533 QSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSL 354
++R+ KNLPPGPP++PIIGNLHHLK PLHRTF LS+KYG + SLWFGSRLV+V+SS S
Sbjct: 25 RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASE 84
Query: 353 VQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSF 174
Q+CF+KNDV+ ANRPRFL+GK+IFYNYTTLGS YG+HWRNLRRIT +DVLSNHR+NSF
Sbjct: 85 FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 144
Query: 173 LGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
GIRRDET+RL+ +L +D ++T FA++EL SRL +MTFN +MRMISGKRY
Sbjct: 145 SGIRRDETQRLITRLADD--------SSTNFAEMELSSRLYDMTFNNIMRMISGKRY 193
>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
Length = 499
Score = 257 bits (656), Expect = 5e-66
Identities = 121/177 (68%), Positives = 148/177 (83%)
Frame = -2
Query: 533 QSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSL 354
++R+ KNLPPGPP++PIIGNLHHLK PLHRTF LS+KYG +ISLWFGSRLV+V+SS S
Sbjct: 25 RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSASE 84
Query: 353 VQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSF 174
Q+CF+KNDV+ ANRPRFL+GK+IFYNYTTLGS YG+HWRNLRRIT +DVLSNHR+NSF
Sbjct: 85 FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 144
Query: 173 LGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
GIRRDET+RL+ +L +D ++T FA++EL R +MTFN +MRMISGKRY
Sbjct: 145 SGIRRDETQRLITRLADD--------SSTNFAEIELSYRFYDMTFNNIMRMISGKRY 193
>ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum]
gi|3850630|emb|CAA10067.1| cytochrome P450 [Cicer
arietinum]
Length = 498
Score = 257 bits (656), Expect = 5e-66
Identities = 124/182 (68%), Positives = 149/182 (81%)
Frame = -2
Query: 548 ILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVI 369
I L QSR+ KNLPPGPP++PIIGNLHHLK PLHRTF LS+ YGDIISLWFGSRLV+V+
Sbjct: 20 IRLLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVV 79
Query: 368 SSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNH 189
SS S Q+CF+KNDV+ ANRPRFL+GK+IFYNYTTLGS Y +HWRNLRRIT++DVLSNH
Sbjct: 80 SSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNH 139
Query: 188 RLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGK 9
R+N+F G+RRDET+RL+ KL D ++T+FA+VEL R +MTFN +MRMI GK
Sbjct: 140 RINNFSGVRRDETQRLITKLAED--------SSTSFAEVELSFRFYDMTFNNIMRMIPGK 191
Query: 8 RY 3
RY
Sbjct: 192 RY 193
>ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arietinum]
gi|4688640|emb|CAB41490.1| cytochrome P450 monooxygenase
[Cicer arietinum]
Length = 498
Score = 256 bits (655), Expect = 7e-66
Identities = 122/177 (68%), Positives = 147/177 (83%)
Frame = -2
Query: 533 QSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSL 354
QSR+ KNLPPGPP++PIIGN HHLK PLHRTF LS+ YGDIISLWFGSRLV+V+SS S
Sbjct: 25 QSRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSE 84
Query: 353 VQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSF 174
Q+CF+KNDV+ ANRPRFL+GK+IFYNYTTLGS Y +HWRNLRRIT++DVLSNHR+N+F
Sbjct: 85 FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRINNF 144
Query: 173 LGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
G+RRDET+RL+ KL D ++T+FA+VEL R +MTFN +MRMISGKRY
Sbjct: 145 SGVRRDETQRLITKLAED--------SSTSFAEVELSFRFYDMTFNNIMRMISGKRY 193
>dbj|BAT21114.1| cytochrome P450 [synthetic construct]
Length = 477
Score = 254 bits (650), Expect = 3e-65
Identities = 122/176 (69%), Positives = 144/176 (81%)
Frame = -2
Query: 530 SRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSLV 351
SR+ KNLPPGP ++PIIGNLHHLK PLHRTF LS+KYGD+ SLWFG+RLV+V+SS + V
Sbjct: 4 SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADV 63
Query: 350 QECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSFL 171
QECF+KNDV+ ANRPRFL+GK+IFYNYTTLGS YG+HWRNLRRIT++DVLSNHR+NSF
Sbjct: 64 QECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFS 123
Query: 170 GIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
IRRDET RL+ KL D K F++VEL SR +MTFN +MRMISGKRY
Sbjct: 124 PIRRDETTRLIRKLAEDSAKN--------FSEVELTSRFFDMTFNNIMRMISGKRY 171
>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
Length = 499
Score = 254 bits (650), Expect = 3e-65
Identities = 122/176 (69%), Positives = 144/176 (81%)
Frame = -2
Query: 530 SRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVISSPSLV 351
SR+ KNLPPGP ++PIIGNLHHLK PLHRTF LS+KYGD+ SLWFG+RLV+V+SS + V
Sbjct: 26 SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADV 85
Query: 350 QECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNHRLNSFL 171
QECF+KNDV+ ANRPRFL+GK+IFYNYTTLGS YG+HWRNLRRIT++DVLSNHR+NSF
Sbjct: 86 QECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFS 145
Query: 170 GIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGKRY 3
IRRDET RL+ KL D K F++VEL SR +MTFN +MRMISGKRY
Sbjct: 146 PIRRDETTRLIRKLAEDSAKN--------FSEVELTSRFFDMTFNNIMRMISGKRY 193
>ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago truncatula]
gi|75293247|sp|Q6WNR0.1|C81E7_MEDTR RecName:
Full=Isoflavone 2'-hydroxylase; AltName:
Full=4'-methoxyisoflavone 2'-hydroxylase; AltName:
Full=Cytochrome P450 81E7 gi|33521517|gb|AAQ20040.1|
isoflavone 2'-hydroxylase [Medicago truncatula]
gi|657389785|gb|KEH31433.1| cytochrome P450 family 81
protein [Medicago truncatula]
Length = 498
Score = 254 bits (649), Expect = 3e-65
Identities = 123/182 (67%), Positives = 150/182 (82%)
Frame = -2
Query: 548 ILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSRLVMVI 369
I L QSR+ KNLPPGP ++PIIGNLHHLK PL+RTF L++KYG++ISLWFGSRLV+V+
Sbjct: 20 IRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVV 79
Query: 368 SSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTIDVLSNH 189
SS S QECF+KNDV+ ANRPRFL+GK+IFYNYTTLGS YG+HWRNLRRIT++DVLSNH
Sbjct: 80 SSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNH 139
Query: 188 RLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMRMISGK 9
R+N+F IRRDET+RL +KK+ E ++T FA+VEL R +MTFN +MRMISGK
Sbjct: 140 RINNFAPIRRDETQRL--------IKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGK 191
Query: 8 RY 3
RY
Sbjct: 192 RY 193
>ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
gi|561032273|gb|ESW30852.1| hypothetical protein
PHAVU_002G187800g [Phaseolus vulgaris]
Length = 500
Score = 251 bits (642), Expect = 2e-64
Identities = 125/187 (66%), Positives = 147/187 (78%)
Frame = -2
Query: 563 ILTVKILLQTQSRRLKNLPPGPPTVPIIGNLHHLKHPLHRTFATLSQKYGDIISLWFGSR 384
I T+K LLQ RRLKNLPP PPT+PIIGNLH +K PLHR+F +LSQKYG I SLWFGSR
Sbjct: 15 IFTLKFLLQR--RRLKNLPPHPPTLPIIGNLHLIKPPLHRSFLSLSQKYGPIFSLWFGSR 72
Query: 383 LVMVISSPSLVQECFSKNDVIFANRPRFLTGKHIFYNYTTLGSAPYGDHWRNLRRITTID 204
V+VI+SP+LVQECF+K D + ANRPR LTGK++FYNYT++GS+PYG+HWRNLRRI TID
Sbjct: 73 YVVVITSPTLVQECFTKYDTVLANRPRLLTGKYLFYNYTSMGSSPYGEHWRNLRRIITID 132
Query: 203 VLSNHRLNSFLGIRRDETKRLVHKLQNDVVKKVPEGAATAFAKVELRSRLTEMTFNAMMR 24
VLS RLNSF IRR+ET R++ KL + K F V++R RLTEMTFN MMR
Sbjct: 133 VLSTQRLNSFFEIRREETMRVIQKLVRETCK--------GFTHVQIRPRLTEMTFNNMMR 184
Query: 23 MISGKRY 3
MISGKRY
Sbjct: 185 MISGKRY 191