BLASTX nr result
ID: Stemona21_contig00031860
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Stemona21_contig00031860
(527 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN71285.1| hypothetical protein VITISV_007002 [Vitis vinifera] 52 3e-13
emb|CAN62201.1| hypothetical protein VITISV_004238 [Vitis vinifera] 48 1e-10
ref|XP_003571702.1| PREDICTED: uncharacterized protein LOC100832... 51 2e-10
gb|EMS64730.1| Vacuolar cation/proton exchanger 3 [Triticum urartu] 68 1e-09
gb|EMT31698.1| Putative serine/threonine-protein kinase receptor... 45 2e-09
ref|XP_004963559.1| PREDICTED: uncharacterized protein LOC101776... 66 4e-09
ref|XP_004960974.1| PREDICTED: uncharacterized protein LOC101782... 66 4e-09
ref|XP_003560256.1| PREDICTED: uncharacterized protein LOC100824... 66 4e-09
emb|CAN69186.1| hypothetical protein VITISV_041146 [Vitis vinifera] 50 5e-09
ref|XP_003581308.1| PREDICTED: uncharacterized protein LOC100844... 66 5e-09
gb|ACN26116.1| unknown [Zea mays] 65 9e-09
ref|XP_004986883.1| PREDICTED: uncharacterized protein LOC101755... 64 2e-08
ref|XP_004971795.1| PREDICTED: putative nuclease HARBI1-like [Se... 64 2e-08
ref|XP_004963970.1| PREDICTED: putative nuclease HARBI1-like [Se... 64 2e-08
ref|XP_004959394.1| PREDICTED: uncharacterized protein LOC101765... 64 2e-08
ref|XP_004980812.1| PREDICTED: uncharacterized protein LOC101772... 63 4e-08
ref|XP_004959007.1| PREDICTED: putative nuclease HARBI1-like [Se... 63 5e-08
ref|XP_004959251.1| PREDICTED: uncharacterized protein LOC101777... 62 6e-08
ref|XP_004980432.1| PREDICTED: uncharacterized protein LOC101776... 62 8e-08
ref|XP_004978313.1| PREDICTED: uncharacterized protein LOC101783... 62 8e-08
>emb|CAN71285.1| hypothetical protein VITISV_007002 [Vitis vinifera]
Length = 641
Score = 52.4 bits (124), Expect(2) = 3e-13
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = +3
Query: 237 TSFWSFFKDCIGAVDDTHITARVSPDIVARFTGRN 341
T FW +FKDCIGA+D +H +VS D+V R+ GRN
Sbjct: 144 TKFWPYFKDCIGAIDGSHFRVKVSNDVVQRYRGRN 178
Score = 48.1 bits (113), Expect(2) = 3e-13
Identities = 22/42 (52%), Positives = 28/42 (66%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPP 205
GH RNR + YF RSSET SR+F +VL+A+ L D F+ P
Sbjct: 90 GHNLRNRTMKFYFKRSSETISRHFHQVLRAIISLDDVFLKQP 131
>emb|CAN62201.1| hypothetical protein VITISV_004238 [Vitis vinifera]
Length = 696
Score = 48.1 bits (113), Expect(2) = 1e-10
Identities = 22/42 (52%), Positives = 28/42 (66%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPP 205
GH RNR + YF RSSET SR+F +VL+A+ L D F+ P
Sbjct: 163 GHNLRNRTMKFYFKRSSETISRHFHQVLRAIISLDDVFLKQP 204
Score = 43.5 bits (101), Expect(2) = 1e-10
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +3
Query: 237 TSFWSFFKDCIGAVDDTHITARVSPDIV 320
T FW +FKDCIGA+D +H +VS D+V
Sbjct: 217 TKFWPYFKDCIGAIDGSHFRVKVSNDVV 244
>ref|XP_003571702.1| PREDICTED: uncharacterized protein LOC100832049 [Brachypodium
distachyon]
Length = 173
Score = 51.2 bits (121), Expect(2) = 2e-10
Identities = 26/49 (53%), Positives = 33/49 (67%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK 226
GH RNRVIG F RS+ET RYFR VL A+ +L+ +I P + EV +K
Sbjct: 62 GHNLRNRVIGANFDRSNETVRRYFRLVLHAIGELRTEYIRPSSLEVPTK 110
Score = 39.7 bits (91), Expect(2) = 2e-10
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = +3
Query: 243 FWSFFKDCIGAVDDTHITARVSPDIVARFTGR 338
F +FKDCIGA+D TH+ A V D+ F GR
Sbjct: 117 FDPYFKDCIGAIDGTHVRASVPKDMEPSFRGR 148
>gb|EMS64730.1| Vacuolar cation/proton exchanger 3 [Triticum urartu]
Length = 782
Score = 67.8 bits (164), Expect = 1e-09
Identities = 45/117 (38%), Positives = 63/117 (53%), Gaps = 1/117 (0%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFRDDLFLVIFQ 259
GH RNRVI + F S ET SR+F++VLKA+ L D +I P +EV ++ D +
Sbjct: 105 GHNVRNRVIQNRFQHSGETISRHFKRVLKAINGLCDVYITDPPNEVPNEILGDSRFYPYF 164
Query: 260 GLHRSGR*HTHNS*GIA*HCCEVYREKHS-PT*NFLTVVNFNLEFSYVLAG*EGSTN 427
TH + I YR +H P+ N + VV+F++ FSYV AG EGS +
Sbjct: 165 KNCMGAIDGTHINAKIKLDKQTPYRNRHGYPSQNVMAVVSFDMTFSYVAAGWEGSAS 221
>gb|EMT31698.1| Putative serine/threonine-protein kinase receptor [Aegilops
tauschii]
Length = 1181
Score = 45.1 bits (105), Expect(2) = 2e-09
Identities = 24/51 (47%), Positives = 32/51 (62%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFR 232
GH Q RVI + F RS ET SRYF+++L AVA+L+ I P+ + K R
Sbjct: 89 GHNQWFRVIHNTFRRSMETISRYFKQMLFAVAELRGEMIRRPSGQTPPKIR 139
Score = 42.0 bits (97), Expect(2) = 2e-09
Identities = 18/37 (48%), Positives = 24/37 (64%)
Frame = +3
Query: 243 FWSFFKDCIGAVDDTHITARVSPDIVARFTGRNIVLR 353
++ +FKDCIGA+D TH+TARV A + GR R
Sbjct: 144 WYPYFKDCIGAIDGTHVTARVPRSQSAAYRGRKYYTR 180
>ref|XP_004963559.1| PREDICTED: uncharacterized protein LOC101776865 [Setaria italica]
Length = 283
Score = 66.2 bits (160), Expect = 4e-09
Identities = 45/122 (36%), Positives = 66/122 (54%), Gaps = 6/122 (4%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFR-----DDLF 244
GH +RNRV+ F RSSET SRYFR VL A+ +L++ I PP+ E +K D F
Sbjct: 128 GHNERNRVVATNFGRSSETVSRYFRIVLNAIGELRNDLIQPPSLETPTKIAGNPRWDPYF 187
Query: 245 LVIFQGLHRSGR*HTHNS*GIA*HCCEVYREKHS-PT*NFLTVVNFNLEFSYVLAG*EGS 421
+ TH ++ +R + S T N + V+F+++F+YVLAG EG+
Sbjct: 188 KDCIGAID-----GTHIRASVSKSMELAFRGRKSFATQNVMAAVDFDMKFTYVLAGWEGT 242
Query: 422 TN 427
T+
Sbjct: 243 TH 244
>ref|XP_004960974.1| PREDICTED: uncharacterized protein LOC101782507 [Setaria italica]
Length = 577
Score = 66.2 bits (160), Expect = 4e-09
Identities = 43/117 (36%), Positives = 63/117 (53%), Gaps = 1/117 (0%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFRDDLFLVIFQ 259
GH RNR++G F RS ET SRYF KVL+A+ +L+ I PP+ + SK D +
Sbjct: 78 GHNLRNRLVGTNFDRSGETVSRYFNKVLRAIGELRGELIRPPSLDTPSKIAGDNRWDPYF 137
Query: 260 GLHRSGR*HTHNS*GIA*HCCEVYREKHS-PT*NFLTVVNFNLEFSYVLAG*EGSTN 427
TH + + +R + S T N + V+F+L+F+YVLAG EG+ +
Sbjct: 138 KDCIGAIDGTHVRASVPKNIEHAFRSRKSFCTQNVMAAVDFDLQFTYVLAGWEGAAH 194
>ref|XP_003560256.1| PREDICTED: uncharacterized protein LOC100824549 [Brachypodium
distachyon]
Length = 764
Score = 66.2 bits (160), Expect = 4e-09
Identities = 46/122 (37%), Positives = 64/122 (52%), Gaps = 6/122 (4%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFR-----DDLF 244
GH RNRVIG F RS+E SRYFR VL A+ +L+ +I PP+ E +K D F
Sbjct: 303 GHNLRNRVIGANFDRSNERVSRYFRLVLHAIGELRAEYIRPPSLETPAKIAGKPRFDPYF 362
Query: 245 LVIFQGLHRSGR*HTHNS*GIA*HCCEVYREKHS-PT*NFLTVVNFNLEFSYVLAG*EGS 421
+ TH ++ +R + S PT N + V+F+L F+YVLAG EG+
Sbjct: 363 KDCIGAID-----GTHVRASVSKDMEPSFRRRKSFPTQNVMAAVDFDLRFTYVLAGWEGT 417
Query: 422 TN 427
+
Sbjct: 418 AH 419
>emb|CAN69186.1| hypothetical protein VITISV_041146 [Vitis vinifera]
Length = 317
Score = 50.1 bits (118), Expect(2) = 5e-09
Identities = 19/34 (55%), Positives = 25/34 (73%)
Frame = +3
Query: 237 TSFWSFFKDCIGAVDDTHITARVSPDIVARFTGR 338
T FW +FKDCIGA+D +H +VS D+V R+ GR
Sbjct: 190 TKFWPYFKDCIGAIDGSHFCVKVSNDVVQRYRGR 223
Score = 35.8 bits (81), Expect(2) = 5e-09
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = +2
Query: 338 KHSPT*NFLTVVNFNLEFSYVLAG*EGST 424
K+ PT NFL +F+L+F+YVL G +GST
Sbjct: 224 KYYPTQNFLAACSFDLKFTYVLPGWQGST 252
>ref|XP_003581308.1| PREDICTED: uncharacterized protein LOC100844952 [Brachypodium
distachyon]
Length = 550
Score = 65.9 bits (159), Expect = 5e-09
Identities = 46/119 (38%), Positives = 63/119 (52%), Gaps = 6/119 (5%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFR-----DDLF 244
GH RNRVIG F +S+ET SRYFR VL A+ +L+ +I PP+ E +K D F
Sbjct: 42 GHNLRNRVIGANFDKSNETVSRYFRLVLHAIGELRAEYISPPSLETPAKIAGNPRFDPYF 101
Query: 245 LVIFQGLHRSGR*HTHNS*GIA*HCCEVYREKHS-PT*NFLTVVNFNLEFSYVLAG*EG 418
+ TH ++ +R + S PT N + V+F+L F+YVLAG EG
Sbjct: 102 KDCIGAID-----GTHVRASVSKDMKPSFRGRKSFPTQNVMAAVDFDLRFTYVLAGWEG 155
>gb|ACN26116.1| unknown [Zea mays]
Length = 298
Score = 65.1 bits (157), Expect = 9e-09
Identities = 45/122 (36%), Positives = 64/122 (52%), Gaps = 6/122 (4%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFR-----DDLF 244
GH RNR++G + RS ET SRYF KVL+A+ +L+ I PP+ E SK D F
Sbjct: 15 GHNLRNRLVGTNYGRSGETVSRYFNKVLRAIGELRAELIRPPSLETPSKIAGNPRWDPYF 74
Query: 245 LVIFQGLHRSGR*HTHNS*GIA*HCCEVYREKHS-PT*NFLTVVNFNLEFSYVLAG*EGS 421
+ TH ++ +R + S + N + V+F+L F+YVLAG EGS
Sbjct: 75 KDCIGAID-----GTHIRASVSIDMEPSFRGRKSYASQNVMAAVDFDLRFTYVLAGWEGS 129
Query: 422 TN 427
T+
Sbjct: 130 TH 131
>ref|XP_004986883.1| PREDICTED: uncharacterized protein LOC101755964 [Setaria italica]
Length = 612
Score = 63.9 bits (154), Expect = 2e-08
Identities = 46/127 (36%), Positives = 65/127 (51%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI ++ RS ET RYF++VL A+ +L+ I P++E K F
Sbjct: 64 GHNQRFRVIHQHWRRSIETVHRYFKEVLYAIGELRQEMIRAPSNETPVKISNSPRWYPYF 123
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH PT N L V+F+L+F+YVLA
Sbjct: 124 KDCVGAI--DGTHVYAR--------VPAKIQAAFRGRKHYPTQNVLATVDFDLKFTYVLA 173
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 174 GWEGSAH 180
>ref|XP_004971795.1| PREDICTED: putative nuclease HARBI1-like [Setaria italica]
Length = 393
Score = 63.9 bits (154), Expect = 2e-08
Identities = 46/127 (36%), Positives = 65/127 (51%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI ++ RS ET RYF++VL A+ +L+ I P++E K F
Sbjct: 110 GHNQRFRVIHQHWRRSIETVHRYFKEVLYAIGELRQEMIRAPSNETPVKISNSPRWYPYF 169
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH PT N L V+F+L+F+YVLA
Sbjct: 170 KDCVGAI--DGTHVYAR--------VPAKIQAAFRGRKHYPTQNVLAAVDFDLKFTYVLA 219
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 220 GWEGSAH 226
>ref|XP_004963970.1| PREDICTED: putative nuclease HARBI1-like [Setaria italica]
Length = 347
Score = 63.9 bits (154), Expect = 2e-08
Identities = 46/127 (36%), Positives = 65/127 (51%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI ++ RS ET RYF++VL A+ +L+ I P++E K F
Sbjct: 64 GHNQRFRVIHQHWRRSIETVHRYFKEVLYAIGELRQEMIRAPSNETPVKISNSPRWYPYF 123
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH PT N L V+F+L+F+YVLA
Sbjct: 124 KDCVGAI--DGTHVYAR--------VPAKIQAAFRGRKHYPTQNVLAAVDFDLKFTYVLA 173
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 174 GWEGSAH 180
>ref|XP_004959394.1| PREDICTED: uncharacterized protein LOC101765357 [Setaria italica]
Length = 999
Score = 63.9 bits (154), Expect = 2e-08
Identities = 46/127 (36%), Positives = 65/127 (51%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI ++ RS ET RYF++VL A+ +L+ I P++E K F
Sbjct: 64 GHNQRFRVIHQHWRRSIETVHRYFKEVLYAIGELRQEMIRAPSNETPVKISNSPRWYPYF 123
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH PT N L V+F+L+F+YVLA
Sbjct: 124 KDCVGAI--DGTHVYAR--------VPAKIQAAFRGRKHYPTQNVLAAVDFDLKFTYVLA 173
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 174 GWEGSAH 180
>ref|XP_004980812.1| PREDICTED: uncharacterized protein LOC101772526 [Setaria italica]
Length = 494
Score = 63.2 bits (152), Expect = 4e-08
Identities = 47/116 (40%), Positives = 59/116 (50%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSKFRDDLFLVIFQ 259
GH QR RVI F RS ET SR+F++VL AV +L+ I PP+ V K +
Sbjct: 67 GHNQRFRVINLSFRRSFETISRHFQEVLYAVGELRQEMIQPPSTVVHPKID---CIGAID 123
Query: 260 GLHRSGR*HTHNS*GIA*HCCEVYREKHSPT*NFLTVVNFNLEFSYVLAG*EGSTN 427
G H R S A KH+ T N L V+F+L F+YVLAG EGS +
Sbjct: 124 GTHVLAR--VPRSIRAA-----FLGRKHTTTQNVLVAVDFDLRFTYVLAGWEGSAH 172
>ref|XP_004959007.1| PREDICTED: putative nuclease HARBI1-like [Setaria italica]
Length = 393
Score = 62.8 bits (151), Expect = 5e-08
Identities = 45/127 (35%), Positives = 65/127 (51%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI ++ RS ET RYF++VL A+ +L+ I P++E K F
Sbjct: 110 GHNQRFRVIHQHWRRSIETVHRYFKEVLYAIGELRQEMIRAPSNETPVKISNSPRWYPYF 169
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH PT N L V+F+++F+YVLA
Sbjct: 170 KDCVGAI--DGTHVYAR--------VPAKIQAAFRGRKHYPTQNVLAAVDFDVKFTYVLA 219
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 220 GWEGSAH 226
>ref|XP_004959251.1| PREDICTED: uncharacterized protein LOC101777656 [Setaria italica]
Length = 625
Score = 62.4 bits (150), Expect = 6e-08
Identities = 47/127 (37%), Positives = 64/127 (50%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI F RS ET SR+F +VL AV +L++ I PP+ V + F
Sbjct: 106 GHNQRFRVINMTFRRSPETISRFFHQVLYAVGELRNELIVPPSTSVHPRILGSRRWNPYF 165
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH+ T N L V+F+L F+YVLA
Sbjct: 166 KDCIGAI--DGTHVLAR--------VPLKMQAAFRGRKHTITQNVLAAVDFDLRFTYVLA 215
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 216 GWEGSAH 222
>ref|XP_004980432.1| PREDICTED: uncharacterized protein LOC101776047 [Setaria italica]
Length = 665
Score = 62.0 bits (149), Expect = 8e-08
Identities = 47/127 (37%), Positives = 64/127 (50%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI F RS ET SR+F +VL AV +L++ I PP+ V + F
Sbjct: 106 GHNQRFRVINMTFRRSIETISRFFHQVLYAVGELRNELIVPPSTSVHPRILGSRRWNPYF 165
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH+ T N L V+F+L F+YVLA
Sbjct: 166 KDCIGAI--DGTHVLAR--------VPLKMQAAFRGRKHTITQNVLAAVDFDLRFTYVLA 215
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 216 GWEGSAH 222
>ref|XP_004978313.1| PREDICTED: uncharacterized protein LOC101783187 [Setaria italica]
Length = 889
Score = 62.0 bits (149), Expect = 8e-08
Identities = 47/127 (37%), Positives = 64/127 (50%), Gaps = 11/127 (8%)
Frame = +2
Query: 80 GHCQRNRVIGHYFMRSSETTSRYFRKVLKAVAKLKDTFIHPPTDEVQSK----------F 229
GH QR RVI F RS ET SR+F +VL AV +L++ I PP+ V + F
Sbjct: 106 GHNQRFRVINMTFRRSIETISRFFHQVLYAVGELRNELIVPPSTSVHPRILGSRRWNPYF 165
Query: 230 RDDLFLVIFQGLHRSGR*HTHNS*GIA*HCCEVYR-EKHSPT*NFLTVVNFNLEFSYVLA 406
+D + + G H R + +R KH+ T N L V+F+L F+YVLA
Sbjct: 166 KDCIGAI--DGTHVLAR--------VPLKMQAAFRGRKHTITQNVLAAVDFDLRFTYVLA 215
Query: 407 G*EGSTN 427
G EGS +
Sbjct: 216 GWEGSAH 222