BLASTX nr result
ID: Sinomenium22_contig00027599
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium22_contig00027599
(1117 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_007024447.1| Pyridoxal phosphate phosphatase-related prot... 374 e-101
ref|XP_006426489.1| hypothetical protein CICLE_v10026096mg [Citr... 365 1e-98
ref|XP_002280889.1| PREDICTED: inorganic pyrophosphatase 2 [Viti... 364 4e-98
ref|XP_006466070.1| PREDICTED: inorganic pyrophosphatase 1-like ... 363 9e-98
ref|NP_001234003.1| psi14B protein [Solanum lycopersicum] gi|269... 357 5e-96
emb|CAD30864.1| putative phosphatase [Solanum lycopersicum] 356 9e-96
ref|XP_007215797.1| hypothetical protein PRUPE_ppa009904mg [Prun... 355 2e-95
ref|NP_001234501.1| psi14A protein [Solanum lycopersicum] gi|269... 354 3e-95
ref|XP_002277629.1| PREDICTED: inorganic pyrophosphatase 2 [Viti... 353 7e-95
ref|NP_001266264.1| inorganic pyrophosphatase 1-like [Solanum ly... 351 3e-94
ref|XP_006357372.1| PREDICTED: inorganic pyrophosphatase 1-like ... 350 5e-94
ref|XP_002280867.1| PREDICTED: inorganic pyrophosphatase 2 [Viti... 350 6e-94
ref|NP_001241414.1| uncharacterized protein LOC100785878 [Glycin... 348 3e-93
ref|XP_006343003.1| PREDICTED: inorganic pyrophosphatase 1-like ... 346 9e-93
ref|XP_007024470.1| Inorganic pyrophosphatase 2 [Theobroma cacao... 346 9e-93
gb|EXB34846.1| Inorganic pyrophosphatase 1 [Morus notabilis] 346 1e-92
ref|XP_006385338.1| hypothetical protein POPTR_0003s02940g [Popu... 345 2e-92
gb|ACU19181.1| unknown [Glycine max] 345 3e-92
ref|XP_003529939.1| PREDICTED: inorganic pyrophosphatase 2-like ... 344 4e-92
ref|XP_002311810.1| hypothetical protein POPTR_0008s20130g [Popu... 343 8e-92
>ref|XP_007024447.1| Pyridoxal phosphate phosphatase-related protein [Theobroma cacao]
gi|508779813|gb|EOY27069.1| Pyridoxal phosphate
phosphatase-related protein [Theobroma cacao]
Length = 274
Score = 374 bits (959), Expect = e-101
Identities = 181/272 (66%), Positives = 221/272 (81%), Gaps = 1/272 (0%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIIDCDSD+WVV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDCDSDNWVVDELGFTDLFNQLLPTMPWNSLMDRMMKELHARGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DDI E L+R PL PRI+ AIK+A+ALGC+LRIVSDAN+FFIE IL+H GL FSEI +N
Sbjct: 61 DDIAEVLKRTPLHPRIVPAIKAANALGCELRIVSDANLFFIETILEHLGLKEYFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT-EGKKRFIYLGDGK 428
P F+D EG+L I PYHDFT HGC L C NMCKG VIERI+A+ EGKK+ IYLGDG
Sbjct: 121 PGFVDGEGRLRIFPYHDFTKCSHGCNL--CPPNMCKGMVIERIQASLEGKKKIIYLGDGS 178
Query: 427 GDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINL 248
GD+C SLKLGE D++MPRKN+PVW LI +NP+LIKA+IHEWS+GEELE+ LL +INII++
Sbjct: 179 GDYCPSLKLGEADYMMPRKNFPVWDLICRNPMLIKAEIHEWSDGEELERLLLQIINIISV 238
Query: 247 DCRNGRNSAALISPECKFQSVPNGIHDPFPQA 152
+ NG +SA LIS +CK Q++ H+ PQA
Sbjct: 239 EENNG-SSAQLISVDCKLQTISASTHEALPQA 269
>ref|XP_006426489.1| hypothetical protein CICLE_v10026096mg [Citrus clementina]
gi|557528479|gb|ESR39729.1| hypothetical protein
CICLE_v10026096mg [Citrus clementina]
Length = 321
Score = 365 bits (938), Expect = 1e-98
Identities = 175/276 (63%), Positives = 216/276 (78%), Gaps = 3/276 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
MSG+VV+FDFDKTIIDCDSD++VV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 47 MSGIVVIFDFDKTIIDCDSDNFVVDELGATDLFNQLLPTMPWNSLMDRMMKELHSQGKTI 106
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E L+R P+ PR++ AIKSAHALGC+LRIVSDAN+FFIE IL+H G+ CFSEI +N
Sbjct: 107 EDILEVLKRIPIHPRVVPAIKSAHALGCELRIVSDANLFFIETILEHLGIRDCFSEINTN 166
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGD 434
P F+D+EG+L I P+HDFT HGC L C NMCKG VIERI+A+ EG K+ IYLGD
Sbjct: 167 PGFVDEEGRLRIFPFHDFTKCSHGCNL--CPPNMCKGVVIERIQASLSKEGNKKIIYLGD 224
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GD+C SLKL E D VMPRKN+P+W LI +NP+LIKA+IHEW++GEELEQ LLHL+N I
Sbjct: 225 GSGDYCPSLKLSEGDHVMPRKNFPLWDLIIRNPMLIKAEIHEWTDGEELEQILLHLVNTI 284
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQAAS 146
N NSA L+S +CK Q++ H+ PQ S
Sbjct: 285 G--STNNNNSAQLLSADCKLQTISAAAHETLPQVLS 318
>ref|XP_002280889.1| PREDICTED: inorganic pyrophosphatase 2 [Vitis vinifera]
gi|297743107|emb|CBI35974.3| unnamed protein product
[Vitis vinifera]
Length = 273
Score = 364 bits (934), Expect = 4e-98
Identities = 175/262 (66%), Positives = 215/262 (82%), Gaps = 2/262 (0%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIID DSD+WVV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDVDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMKELHSQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DDI E L+R P+ PR++ AIKSAHALGCDL+IVSDAN+FFIE ILKH G+M CFSEI +N
Sbjct: 61 DDIAEVLKRIPIHPRVVPAIKSAHALGCDLKIVSDANLFFIETILKHLGVMDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKATEGKKRFIYLGDGKG 425
PSF+D+EG+L I P+HDFTSS HGC L C NMCKG VI+RI+A+ ++FIYLGDG G
Sbjct: 121 PSFVDEEGRLRIFPHHDFTSSSHGCSL--CPPNMCKGMVIKRIQASISTEKFIYLGDGSG 178
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC SLKLG+ D+VMPRKN+P+W LI +NP LIKA++HEWS+GEELE LLHLI I+ +
Sbjct: 179 DFCPSLKLGDGDYVMPRKNFPLWDLICRNPNLIKAEVHEWSDGEELEHGLLHLIKKISEE 238
Query: 244 CRNGRNSAA--LISPECKFQSV 185
NSAA LIS +CKF+++
Sbjct: 239 NNANNNSAAAQLISVDCKFETM 260
>ref|XP_006466070.1| PREDICTED: inorganic pyrophosphatase 1-like [Citrus sinensis]
Length = 275
Score = 363 bits (931), Expect = 9e-98
Identities = 174/276 (63%), Positives = 215/276 (77%), Gaps = 3/276 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
MSG+VV+FDFDKTIIDCDSD++VV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MSGIVVIFDFDKTIIDCDSDNFVVDELGATDLFNQLLPTMPWNSLMDRMMKELHSQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E L+R P+ PR++ AIKSAHALGC+LRIVSDAN+FFIE IL+H G+ CFSEI +N
Sbjct: 61 EDIVEVLKRIPIHPRVVPAIKSAHALGCELRIVSDANLFFIETILEHLGIRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGD 434
P F+D+EG+L I P+HDFT HGC L C NMCKG VIERI+A+ EG K+ IYLGD
Sbjct: 121 PGFVDEEGRLRIFPFHDFTKCSHGCNL--CPPNMCKGVVIERIQASLSKEGNKKIIYLGD 178
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GD+C SLKL E D VMPRKN+P+W LI +NP+LIKA+I EW++GEELEQ LLHL+N I
Sbjct: 179 GSGDYCPSLKLSEGDHVMPRKNFPLWDLIIRNPMLIKAEIREWTDGEELEQILLHLVNTI 238
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQAAS 146
N NSA L+S +CK Q++ H+ PQ S
Sbjct: 239 G--STNNNNSAQLLSADCKLQTISAAAHETLPQVLS 272
>ref|NP_001234003.1| psi14B protein [Solanum lycopersicum] gi|26986118|emb|CAD30862.1|
putative phosphatase [Solanum lycopersicum]
gi|26986124|emb|CAD30865.1| putative phosphatase
[Solanum lycopersicum]
Length = 269
Score = 357 bits (916), Expect = 5e-96
Identities = 173/270 (64%), Positives = 205/270 (75%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIID DSD+WVV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDVDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMKELHTQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DI E L+R P+ PRI+ AIKSAHALGCDLR++SDANVFFIE ILKH G+ CFSEI +N
Sbjct: 61 QDIEEVLKRVPIHPRIVPAIKSAHALGCDLRVISDANVFFIETILKHLGIRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKATEGKKRFIYLGDGKG 425
P ++D EG+L ILPY DF SPHGC L C NMCKG ++ERI+A EGKKR IYLGDG G
Sbjct: 121 PGYVDGEGRLRILPYVDFQKSPHGCNL--CPPNMCKGMIVERIQAKEGKKRMIYLGDGIG 178
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC SLKL E DFVMPRK++P W LI KN L+KA +HEW+NG+ELE LL IN IN++
Sbjct: 179 DFCPSLKLREADFVMPRKDFPAWNLINKNRTLVKAGVHEWTNGKELEHILLQWINTINIE 238
Query: 244 CRNGRNSAALISPECKFQSVPNGIHDPFPQ 155
S L CKFQ+ PN H P+
Sbjct: 239 -----ESQLLSMEYCKFQTKPNADHGALPR 263
>emb|CAD30864.1| putative phosphatase [Solanum lycopersicum]
Length = 269
Score = 356 bits (914), Expect = 9e-96
Identities = 172/271 (63%), Positives = 204/271 (75%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIID DSD+WVV ELG TDLFN LL TMPWN+LMNR+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDVDSDNWVVDELGATDLFNQLLPTMPWNSLMNRMMKELHTQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DI E L+R P+ PRI+ AIKSAHALGCDLR++SDANVFF+E ILKH G+ CFSEI +N
Sbjct: 61 QDIEEVLKRVPIHPRIVPAIKSAHALGCDLRVISDANVFFLETILKHLGIRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKATEGKKRFIYLGDGKG 425
P ++D EG+L ILPY DF SPHGC L C NMCKG ++ERI+A EGKKR IYLGDG G
Sbjct: 121 PGYVDGEGRLRILPYVDFQKSPHGCNL--CPPNMCKGMIVERIQAKEGKKRMIYLGDGIG 178
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC SLKL E DFVMPRK++P W LI KN L+KA +HEW+NG+ELE LL LIN IN++
Sbjct: 179 DFCPSLKLSEADFVMPRKDFPAWNLINKNRTLVKATVHEWTNGQELEHILLQLINTINME 238
Query: 244 CRNGRNSAALISPECKFQSVPNGIHDPFPQA 152
S L CK ++ H PQA
Sbjct: 239 -----ESQLLSVDYCKLVTMSKAAHGALPQA 264
>ref|XP_007215797.1| hypothetical protein PRUPE_ppa009904mg [Prunus persica]
gi|462411947|gb|EMJ16996.1| hypothetical protein
PRUPE_ppa009904mg [Prunus persica]
Length = 272
Score = 355 bits (911), Expect = 2e-95
Identities = 174/274 (63%), Positives = 216/274 (78%), Gaps = 3/274 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTII+CDSD+WVV ELG TDLFN LL TMPWN+LM+R+MEE+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIECDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMEELHSQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E L+R P+ PR++ AIK+AHALGCDLRIVSDAN+FFIE IL H GL FSEI +N
Sbjct: 61 EDIAEVLKRTPIHPRVVPAIKAAHALGCDLRIVSDANLFFIETILNHLGLEEYFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIK---ATEGKKRFIYLGD 434
PS++D++G+L I P+HDFT HGC L C NMCKG VIERI+ +TEGKK+ IYLGD
Sbjct: 121 PSYVDEQGRLRISPHHDFTKFSHGCSL--CPPNMCKGVVIERIQTSVSTEGKKKIIYLGD 178
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GD+C SLKL E DFVMPRKN+P++ LI K+PLLIKA IHEW++GEELEQ LL LIN I
Sbjct: 179 GSGDYCPSLKLKEGDFVMPRKNFPLFDLICKDPLLIKAGIHEWTDGEELEQILLSLINTI 238
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQA 152
+++ +A IS +CK Q++ H+ FP+A
Sbjct: 239 SME-----ENAQFISADCKLQTISASAHEAFPKA 267
>ref|NP_001234501.1| psi14A protein [Solanum lycopersicum] gi|26986120|emb|CAD30863.1|
putative phosphatase [Solanum lycopersicum]
Length = 269
Score = 354 bits (909), Expect = 3e-95
Identities = 171/271 (63%), Positives = 204/271 (75%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIID DSD+WVV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDVDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMKELHTQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DI E L+R P+ PRI+ AIKSAHALGCDLR++SDANVFF+E ILKH G+ CFSEI +N
Sbjct: 61 QDIEEVLKRVPIHPRIVPAIKSAHALGCDLRVISDANVFFLETILKHLGIRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKATEGKKRFIYLGDGKG 425
P ++D EG+L ILPY DF SPHGC L C NMCKG ++ERI+A EGKKR IYLGDG G
Sbjct: 121 PGYVDGEGRLRILPYVDFQKSPHGCNL--CPPNMCKGMIVERIQAKEGKKRMIYLGDGIG 178
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC SLKL E DFVMPRK++P W LI KN L+KA +HEW+NG+ELE LL LIN IN++
Sbjct: 179 DFCPSLKLSEADFVMPRKDFPAWNLINKNRTLVKATVHEWTNGQELEHILLQLINTINME 238
Query: 244 CRNGRNSAALISPECKFQSVPNGIHDPFPQA 152
S L CK ++ H PQA
Sbjct: 239 -----ESQLLSVDYCKLVTMSKAAHGALPQA 264
>ref|XP_002277629.1| PREDICTED: inorganic pyrophosphatase 2 [Vitis vinifera]
gi|296081623|emb|CBI20628.3| unnamed protein product
[Vitis vinifera]
Length = 269
Score = 353 bits (906), Expect = 7e-95
Identities = 173/264 (65%), Positives = 208/264 (78%), Gaps = 7/264 (2%)
Frame = -1
Query: 955 VVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTTDDI 776
VVVVFDFDKTIIDCDSD+WVV ELG+T LF LL TMPWN+LM+R+ E+H +GKT ++I
Sbjct: 3 VVVVFDFDKTIIDCDSDNWVVDELGLTPLFTQLLPTMPWNSLMDRMFMELHSQGKTMEEI 62
Query: 775 TECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSNPSF 596
ECL+RAPL PRIISAIKSAHA GCDLRIVSDAN FFIE +LKH+GLM CFSEI +NPSF
Sbjct: 63 AECLKRAPLHPRIISAIKSAHASGCDLRIVSDANAFFIETVLKHHGLMDCFSEIKTNPSF 122
Query: 595 IDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIK---ATEGKKRFIYLGDGKG 425
+D EG+L ILPYHD SS HGC C NMCKG V+ER++ + EGKKRFIYLGDG
Sbjct: 123 VDGEGRLRILPYHDLKSSSHGC--NFCPPNMCKGLVMERVRTSVSAEGKKRFIYLGDGSA 180
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC+ LKLGE D++MPRKNYP+W++I NP LIK+KI+EWSNGEELE+ LLHLI I+++
Sbjct: 181 DFCSGLKLGEGDYLMPRKNYPIWEIICSNPKLIKSKINEWSNGEELEKILLHLITTISIE 240
Query: 244 ----CRNGRNSAALISPECKFQSV 185
R+ + A+ + FQSV
Sbjct: 241 EKCNMRSDKCQASSMDSHKTFQSV 264
>ref|NP_001266264.1| inorganic pyrophosphatase 1-like [Solanum lycopersicum]
gi|11837862|gb|AAG40473.1|AF305968_1 putative acid
phosphatase [Solanum lycopersicum]
gi|26986116|emb|CAD30861.1| putative phosphatase
[Solanum lycopersicum] gi|26986126|emb|CAD30866.1|
putative phosphatase [Solanum lycopersicum]
Length = 269
Score = 351 bits (901), Expect = 3e-94
Identities = 170/270 (62%), Positives = 203/270 (75%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTII+ DSD+WVV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIEVDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMKELHTQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DI E L+R P+ PRI+ AIKSAHALGCDLR++SDANVFFIE ILKH G+ CFSEI +N
Sbjct: 61 QDIEEVLKRVPIHPRIVPAIKSAHALGCDLRVISDANVFFIETILKHLGIRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKATEGKKRFIYLGDGKG 425
P ++D EG+L ILPY DF SPH C L C NMCKG ++ERI+A EGKKR IYLGDG G
Sbjct: 121 PGYVDGEGRLRILPYVDFQKSPHSCNL--CPPNMCKGMIVERIQAKEGKKRMIYLGDGIG 178
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC SLKL E DFVMPRK++P W LI KN L+KA +HEW+NG+ELE LL IN IN++
Sbjct: 179 DFCPSLKLREADFVMPRKDFPAWNLINKNRTLVKAGVHEWTNGKELEHILLQWINTINIE 238
Query: 244 CRNGRNSAALISPECKFQSVPNGIHDPFPQ 155
S L CKFQ+ N H P+
Sbjct: 239 -----ESQLLSMENCKFQTKHNAAHGALPR 263
>ref|XP_006357372.1| PREDICTED: inorganic pyrophosphatase 1-like [Solanum tuberosum]
Length = 272
Score = 350 bits (899), Expect = 5e-94
Identities = 172/275 (62%), Positives = 211/275 (76%), Gaps = 4/275 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VV FDFDKTIID DSD+WVV ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVFFDFDKTIIDVDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMKELHTQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DI E L+R P PRI+ AIKSA+ALGCDLR++SDANVFFIE ILKH G+ CFSEI +N
Sbjct: 61 QDIEEVLKRVPRHPRIVPAIKSAYALGCDLRVISDANVFFIETILKHVGIRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGD 434
P ++D+EG+L ILPY DF +SPHGC L C NMCKG ++ERI+A+ E K+R IYLGD
Sbjct: 121 PGYVDEEGRLRILPYVDFQTSPHGCNL--CPPNMCKGMIVERIQASIAKEAKRRMIYLGD 178
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GDFC SLKL E DFVMPRK++P W LI KN L+KA +HEW++GEELE+ LL LIN I
Sbjct: 179 GIGDFCPSLKLREGDFVMPRKDFPAWNLINKNRTLVKAAVHEWTDGEELERILLQLINTI 238
Query: 253 NLDCRNGRNSAALISPE-CKFQSVPNGIHDPFPQA 152
N++ + L+S E CKFQ++P H PQA
Sbjct: 239 NIE------ESQLLSVEFCKFQTIPKAAHGALPQA 267
>ref|XP_002280867.1| PREDICTED: inorganic pyrophosphatase 2 [Vitis vinifera]
gi|297743106|emb|CBI35973.3| unnamed protein product
[Vitis vinifera]
Length = 273
Score = 350 bits (898), Expect = 6e-94
Identities = 172/262 (65%), Positives = 206/262 (78%), Gaps = 2/262 (0%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIID DSD+WVV ELG TDLFN LL TMPWN+LM+R+M E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDVDSDNWVVDELGATDLFNQLLPTMPWNSLMDRMMRELHSQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DDI E L+R P+ PR + AIKSAHALGCDL+IVSDAN+FFIE ILKH GL CFSEI +N
Sbjct: 61 DDIAEVLKRVPIHPRAVPAIKSAHALGCDLKIVSDANLFFIETILKHLGLEDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKATEGKKRFIYLGDGKG 425
PSF+D+EG+L I P+HDFTSS HGC L C NMCK VI+RI+A+ ++ IYLGDG G
Sbjct: 121 PSFVDEEGRLRIFPHHDFTSSSHGCSL--CPPNMCKSMVIKRIQASISTEKLIYLGDGSG 178
Query: 424 DFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINLD 245
DFC S KLG D+VMPRKN+P+W LI +NP LIKA+IHEWS+GEELE LLHLI I +
Sbjct: 179 DFCPSSKLGAGDYVMPRKNFPLWDLICRNPNLIKAEIHEWSDGEELELVLLHLIKKIPEE 238
Query: 244 CRNGRNSAA--LISPECKFQSV 185
NSAA LI +CKF+++
Sbjct: 239 NNPNNNSAAAQLIPVDCKFETM 260
>ref|NP_001241414.1| uncharacterized protein LOC100785878 [Glycine max]
gi|255641141|gb|ACU20848.1| unknown [Glycine max]
Length = 272
Score = 348 bits (892), Expect = 3e-93
Identities = 169/274 (61%), Positives = 211/274 (77%), Gaps = 3/274 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
MSG V+VFDFDKTI+D DSD+WV+ ELG TDLFN LL TMPWN+LM+R+M E+H GKT
Sbjct: 1 MSGTVIVFDFDKTIVDVDSDNWVIDELGFTDLFNQLLPTMPWNSLMDRMMMELHSNGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E LRR PL PR+I A+++AHALGCDLRIVSDANVFFIE ILKH G+ FSEI +N
Sbjct: 61 EDIEEVLRRIPLHPRVIPALQAAHALGCDLRIVSDANVFFIETILKHLGIREYFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIK---ATEGKKRFIYLGD 434
P ++++EG+L ILPYHDF + HGC L C NMCKG +I+RI+ + EGKKR IYLGD
Sbjct: 121 PGYVNEEGRLRILPYHDFNKASHGCTL--CPPNMCKGLIIDRIQDSISEEGKKRMIYLGD 178
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GD+C SL+L E DF+MPRKN+PVW LI K+PLL+KA+IH WS+GEELEQ LLHLIN I
Sbjct: 179 GSGDYCPSLRLKERDFMMPRKNFPVWDLICKDPLLVKAEIHGWSDGEELEQVLLHLINKI 238
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQA 152
+++ +A IS +CK Q++ + P+A
Sbjct: 239 SME-----ENAQFISSDCKLQTLSVSALEGLPKA 267
>ref|XP_006343003.1| PREDICTED: inorganic pyrophosphatase 1-like [Solanum tuberosum]
Length = 271
Score = 346 bits (888), Expect = 9e-93
Identities = 169/275 (61%), Positives = 214/275 (77%), Gaps = 4/275 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+G+VVVFDFDKTIID DSD+WV+ ELG TDLFN LL TMPWN+LM+R+M+E+H +GKT
Sbjct: 1 MAGIVVVFDFDKTIIDLDSDNWVIDELGATDLFNRLLPTMPWNSLMDRMMKELHDQGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
DDI + L+R P+ PR++ AIK+AHALGCDLRIVSDANVF+IE ILKH G+ CF+EI +N
Sbjct: 61 DDIEQVLKRVPVIPRVVPAIKAAHALGCDLRIVSDANVFYIETILKHLGIYDCFTEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGD 434
P ++D+EGKL ILP+HDF HGC TC NMCKG +IERI+A+ EGKKR IYLGD
Sbjct: 121 PGYVDEEGKLRILPHHDF---HHGCTFNTCPPNMCKGLIIERIQASLAKEGKKRMIYLGD 177
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GDFC SLKL E+DFVMPRK++PVW+LI KN LI+A+IH WS+GEE +Q LL +I I
Sbjct: 178 GAGDFCPSLKLKEQDFVMPRKDFPVWKLINKNRHLIRARIHGWSDGEEQQQILLQIIKAI 237
Query: 253 NLDCRNGRNSAALISPECKFQSVP-NGIHDPFPQA 152
++ + L + CKFQ++P N +H+ P+A
Sbjct: 238 TIE-----ENQILSTDCCKFQTIPINTVHEALPKA 267
>ref|XP_007024470.1| Inorganic pyrophosphatase 2 [Theobroma cacao]
gi|508779836|gb|EOY27092.1| Inorganic pyrophosphatase 2
[Theobroma cacao]
Length = 279
Score = 346 bits (888), Expect = 9e-93
Identities = 172/279 (61%), Positives = 208/279 (74%), Gaps = 3/279 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M +VV+FDFDKTIIDCDSD+WV+ ELG T LFN LL TMPWN LM+R+M+E+H +G
Sbjct: 1 MVDIVVIFDFDKTIIDCDSDNWVLDELGATKLFNQLLPTMPWNPLMDRMMKELHSQGTKI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI L+R P+ PRII AIKSA+ALGCDL+IVSDAN FFIE ILKH+GL FSEI +N
Sbjct: 61 EDIKAVLKRTPIHPRIIQAIKSAYALGCDLKIVSDANAFFIETILKHHGLREYFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGD 434
P F+D+EG+L I P+HDFT SPHGC C NMCKG VIERI+A+ +GKK IYLGD
Sbjct: 121 PGFVDEEGRLRIFPHHDFTQSPHGC-HHPCPPNMCKGTVIERIQASMSMKGKKTIIYLGD 179
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GDFC SLKLG+ D+VMPRKN+PVW LI +N LIKA+I EWSNGEE E LLH+IN I
Sbjct: 180 GVGDFCPSLKLGDGDYVMPRKNFPVWDLICENRRLIKAEICEWSNGEEFEHVLLHVINRI 239
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQAASQVP 137
++D N N+A L S +CK Q++P + A VP
Sbjct: 240 SIDRNNSDNTARLYSIDCKLQTLPGAAQEKAFSHALYVP 278
>gb|EXB34846.1| Inorganic pyrophosphatase 1 [Morus notabilis]
Length = 270
Score = 346 bits (887), Expect = 1e-92
Identities = 166/263 (63%), Positives = 208/263 (79%), Gaps = 2/263 (0%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
M+ ++VVFDFDKTII+CDSD+WVV+ELG TDLFN LL TMPWN+LM+R+M+E+H GKT
Sbjct: 1 MADIIVVFDFDKTIIECDSDNWVVEELGYTDLFNQLLPTMPWNSLMDRMMKELHELGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E L+R P+ PR++ AI +AHALGCDLRIVSDAN+FFIE IL+H GL CFSEI +N
Sbjct: 61 EDIVEVLKRTPIHPRVVPAITAAHALGCDLRIVSDANLFFIETILEHLGLRDCFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT--EGKKRFIYLGDG 431
P F+D+EG+L ILP+HDF HGC C NMCKG ++ERI+A+ EG KR IYLGDG
Sbjct: 121 PGFVDEEGRLRILPFHDFNKGSHGCP-GLCPPNMCKGVILERIQASLAEGNKRLIYLGDG 179
Query: 430 KGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIIN 251
GD+C SL+L E DFVMPRKN+PVW LI KNPLLIKAKIHEW++GEELE+ LL LI I+
Sbjct: 180 VGDYCPSLRLKEADFVMPRKNFPVWDLISKNPLLIKAKIHEWTDGEELEKILLSLIETIS 239
Query: 250 LDCRNGRNSAALISPECKFQSVP 182
+ +N+AA IS + + ++P
Sbjct: 240 FE----KNAAAFISTDYRLPTIP 258
>ref|XP_006385338.1| hypothetical protein POPTR_0003s02940g [Populus trichocarpa]
gi|550342280|gb|ERP63135.1| hypothetical protein
POPTR_0003s02940g [Populus trichocarpa]
Length = 283
Score = 345 bits (886), Expect = 2e-92
Identities = 170/274 (62%), Positives = 208/274 (75%), Gaps = 3/274 (1%)
Frame = -1
Query: 958 GVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTTDD 779
G+VVVFDFDKTIID DSD+WV+ ELG TDLFN LL TMPWN+LM+R+M+EIH GKT +D
Sbjct: 10 GIVVVFDFDKTIIDVDSDNWVIDELGFTDLFNQLLHTMPWNSLMDRMMKEIHSHGKTIED 69
Query: 778 ITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSNPS 599
I E LRR P+ P++ISAIK+AHALGC+LRIVSDAN+FFIE IL H GL FSEI +NP
Sbjct: 70 IAEVLRRIPIHPQVISAIKAAHALGCELRIVSDANMFFIETILNHLGLKDYFSEINTNPG 129
Query: 598 FIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGDGK 428
F+D++ +L I PYHDFT S H C L C NMCKG +IERI+A+ +G K+ IYLGDG
Sbjct: 130 FVDEQERLRISPYHDFTQSSHCCSL--CPPNMCKGLIIERIQASISKDGSKKIIYLGDGA 187
Query: 427 GDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIINL 248
GD+C SLKL E D+VMPRKN+PVW LI +NPLL+KA+IHEW NG ELE+ LL +I I+
Sbjct: 188 GDYCPSLKLTEADYVMPRKNFPVWDLISENPLLVKAEIHEWINGAELERVLLQIIERIST 247
Query: 247 DCRNGRNSAALISPECKFQSVPNGIHDPFPQAAS 146
D NSA L+S +CK Q++ H+ PQ S
Sbjct: 248 D-EISSNSAQLLSADCKLQTISIAAHEGLPQPLS 280
>gb|ACU19181.1| unknown [Glycine max]
Length = 272
Score = 345 bits (884), Expect = 3e-92
Identities = 168/274 (61%), Positives = 210/274 (76%), Gaps = 3/274 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
MSG V+VFDFDKTI+D DSD+WV+ ELG TDLFN LL TMPWN+LM+R+M E+H GKT
Sbjct: 1 MSGTVIVFDFDKTIVDVDSDNWVIDELGFTDLFNQLLPTMPWNSLMDRMMMELHSNGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E LRR PL PR+I A+++AHALGCDLRIVSDANVFFIE ILKH G+ FSEI +N
Sbjct: 61 EDIEEVLRRIPLHPRVIPALQAAHALGCDLRIVSDANVFFIETILKHLGIREYFSEINTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIK---ATEGKKRFIYLGD 434
P ++++EG+L ILPYHDF + HGC L C NMCKG +I+RI+ + EGKKR IYLGD
Sbjct: 121 PGYVNEEGRLRILPYHDFNKASHGCTL--CPPNMCKGLIIDRIQDSISEEGKKRMIYLGD 178
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GD+C SL+L E DF+M RKN+PVW LI K+PLL+KA+IH WS+GEELEQ LLHLIN I
Sbjct: 179 GSGDYCPSLRLKERDFMMSRKNFPVWDLICKDPLLVKAEIHGWSDGEELEQVLLHLINKI 238
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQA 152
+++ +A IS +CK Q++ + P+A
Sbjct: 239 SME-----ENAQFISSDCKLQTLSVSALEGLPKA 267
>ref|XP_003529939.1| PREDICTED: inorganic pyrophosphatase 2-like [Glycine max]
Length = 272
Score = 344 bits (882), Expect = 4e-92
Identities = 166/273 (60%), Positives = 209/273 (76%), Gaps = 3/273 (1%)
Frame = -1
Query: 964 MSGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTT 785
MSG+VVVFDFDKTI+D DSD+WV+ ELG TDLFN LL TMPWN+LM+++M E+H GKT
Sbjct: 1 MSGIVVVFDFDKTIVDVDSDNWVIDELGFTDLFNQLLPTMPWNSLMDKMMMELHSNGKTI 60
Query: 784 DDITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSN 605
+DI E L R PL PR+I AI++AH LGCDLRIVSDAN+FFIE ILKH G+ FSEI +N
Sbjct: 61 EDIEEVLHRIPLHPRVIPAIQAAHTLGCDLRIVSDANMFFIETILKHLGIREYFSEISTN 120
Query: 604 PSFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGD 434
P ++++E +L ILPYHDF + HGC L C NMCKG +I+RI+ + EGKKR IYLGD
Sbjct: 121 PGYVNEEERLRILPYHDFNKASHGCSL--CPPNMCKGLIIDRIQDSILEEGKKRMIYLGD 178
Query: 433 GKGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINII 254
G GD+C SL+L E DF+MPRKN+PVW LI K+PLL+KA+IH WS+GEELEQ LLHLIN I
Sbjct: 179 GSGDYCPSLRLKERDFMMPRKNFPVWDLICKDPLLVKAEIHGWSDGEELEQVLLHLINKI 238
Query: 253 NLDCRNGRNSAALISPECKFQSVPNGIHDPFPQ 155
+++ +A IS +CK Q++ H+ P+
Sbjct: 239 SME-----ENAQFISSDCKLQTLSVSAHEDLPK 266
>ref|XP_002311810.1| hypothetical protein POPTR_0008s20130g [Populus trichocarpa]
gi|222851630|gb|EEE89177.1| hypothetical protein
POPTR_0008s20130g [Populus trichocarpa]
Length = 277
Score = 343 bits (880), Expect = 8e-92
Identities = 169/275 (61%), Positives = 210/275 (76%), Gaps = 3/275 (1%)
Frame = -1
Query: 961 SGVVVVFDFDKTIIDCDSDDWVVKELGMTDLFNDLLFTMPWNTLMNRIMEEIHLKGKTTD 782
+ +VVVFDFDKTIID DSD+WV+ ELG T+ FN LL TM WN+LM+R+M+E+H GKT +
Sbjct: 3 AAIVVVFDFDKTIIDVDSDNWVIDELGFTESFNQLLPTMSWNSLMDRMMKELHANGKTME 62
Query: 781 DITECLRRAPLSPRIISAIKSAHALGCDLRIVSDANVFFIENILKHYGLMGCFSEICSNP 602
DI E L+R P+ P++ISAIK+AHALGC+LRIVSDAN+FFIE ILKH GL FSEI +NP
Sbjct: 63 DIAEVLKRIPIHPQVISAIKAAHALGCELRIVSDANMFFIETILKHLGLKDYFSEINTNP 122
Query: 601 SFIDKEGKLSILPYHDFTSSPHGCVLETCSLNMCKGQVIERIKAT---EGKKRFIYLGDG 431
F+D+EG+L I PYHDFT S HGC L C NMCKG +IERI+A+ EG K+ IYLGDG
Sbjct: 123 GFVDEEGRLRISPYHDFTQSSHGCSL--CPPNMCKGLIIERIQASISKEGSKKIIYLGDG 180
Query: 430 KGDFCASLKLGEEDFVMPRKNYPVWQLIYKNPLLIKAKIHEWSNGEELEQTLLHLINIIN 251
GD+C SLKL E D++MPRKN+PVW LI KNP LIKA+IHEW++G E+E+ LL +I I+
Sbjct: 181 AGDYCPSLKLTEADYMMPRKNFPVWDLISKNPKLIKAEIHEWNDGAEMERVLLQIIERIS 240
Query: 250 LDCRNGRNSAALISPECKFQSVPNGIHDPFPQAAS 146
+ N NSA L S +CK Q++ HD PQA S
Sbjct: 241 REEINS-NSAKLFSADCKLQTISIAGHDAMPQALS 274