BLASTX nr result
ID: Sinomenium22_contig00021569
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium22_contig00021569
(1346 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap... 306 1e-80
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 306 1e-80
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 304 7e-80
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 303 1e-79
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 298 3e-78
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 293 2e-76
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ... 284 7e-74
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 281 5e-73
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc... 281 6e-73
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 280 1e-72
gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Pap... 278 5e-72
gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum] gi|388... 275 3e-71
ref|XP_006858228.1| hypothetical protein AMTR_s00062p00190100 [A... 273 1e-70
gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 269 2e-69
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 269 2e-69
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe... 269 2e-69
ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase ... 263 2e-67
sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf... 262 3e-67
gb|ACN88562.1| norreticuline-7-O-methyltransferase [Papaver somn... 262 3e-67
emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera] 261 5e-67
>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 346
Score = 306 bits (785), Expect = 1e-80
Identities = 153/342 (44%), Positives = 224/342 (65%), Gaps = 4/342 (1%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPT-VNQDRL 980
D + QA++W+ +YGFAESL LKCA+QL I + L + +PM+LSELA LP+P VN+DRL
Sbjct: 8 DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVPQPVNEDRL 67
Query: 979 FRIMRYLVHMGLFDLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYEMD---AWHHLS 809
FRIMRYLVHM LF + + +KY+L P + L++G +KS+ ++L + D WHHL
Sbjct: 68 FRIMRYLVHMELFKIDATTQKYSLAPPAKYLLRGWEKSMVD-SILCINDKDFLAPWHHLG 126
Query: 808 AAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXXX 629
+ G +EK G Y +++ NQL + AM T +VT AL CK
Sbjct: 127 DGLTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI-FSDGI 185
Query: 628 XXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETDV 449
A + ++KAFP IKC ++DLPHVIA +PE + +I GDMF S+P D
Sbjct: 186 KTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPSADA 245
Query: 448 VFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMDM 269
+FMK +LHDW DE+C++ILK+CKEA+ K GKV+I+D+V+D +S++ + RL +++DM
Sbjct: 246 IFMKCILHDWNDEECIQILKRCKEAL--PKDGKVIIVDVVIDMDSTHPYAKIRLTLDLDM 303
Query: 268 LVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
++ GGKER++EEW+ LF AAG++ +K+T I A++S+IE +P
Sbjct: 304 MLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 345
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=6OMT
gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
6-O-methyltransferase [Papaver somniferum]
gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
6-O-methyltransferase [synthetic construct]
Length = 346
Score = 306 bits (784), Expect = 1e-80
Identities = 153/342 (44%), Positives = 224/342 (65%), Gaps = 4/342 (1%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPT-VNQDRL 980
D + QA++W+ +YGFAESL LKCA+QL I + L + +PM+LSELA LP+ VN+DRL
Sbjct: 8 DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRL 67
Query: 979 FRIMRYLVHMGLFDLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYEMD---AWHHLS 809
FRIMRYLVHM LF + + +KY+L P + L++G +KS+ ++L + D WHHL
Sbjct: 68 FRIMRYLVHMELFKIDATTQKYSLAPPAKYLLRGWEKSMVD-SILCINDKDFLAPWHHLG 126
Query: 808 AAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXXX 629
+ G +EK G Y + + NQL + AM T +VT AL CK
Sbjct: 127 DGLTGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKSI-FSDGI 185
Query: 628 XXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETDV 449
A +A++KAFP IKC ++DLPHVIA +PE + +I GDMF S+P D
Sbjct: 186 STLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPSADA 245
Query: 448 VFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMDM 269
+FMK +LHDW D++C++ILK+CKEA+ KGGKV+I+D+V+D +S++ + RL +++DM
Sbjct: 246 IFMKCILHDWNDDECIQILKRCKEAL--PKGGKVIIVDVVIDMDSTHPYAKIRLTLDLDM 303
Query: 268 LVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
++ GGKER++EEW+ LF AAG++ +K+T I A++S+IE +P
Sbjct: 304 MLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase [Papaver
somniferum]
Length = 346
Score = 304 bits (778), Expect = 7e-80
Identities = 152/342 (44%), Positives = 224/342 (65%), Gaps = 4/342 (1%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPT-VNQDRL 980
D + QA++W+ +YGFAESL LKCA+QL I + L + +PM+LSELA LP+ VN+DRL
Sbjct: 8 DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRL 67
Query: 979 FRIMRYLVHMGLFDLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYEMD---AWHHLS 809
FRIMRYLVHM LF + + +KY+L P + L++G +KS+ ++L + D WHHL
Sbjct: 68 FRIMRYLVHMELFKIDATTQKYSLAPPAKYLLRGWEKSMVD-SILCINDKDFLAPWHHLG 126
Query: 808 AAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXXX 629
+ G +EK G Y +++ NQL + AM T +VT AL CK
Sbjct: 127 DGLTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI-FSDGI 185
Query: 628 XXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETDV 449
A +A++KAFP IKC ++DLPHVIA +PE + +I GDMF S+P D
Sbjct: 186 STLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPSADA 245
Query: 448 VFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMDM 269
+FMK +LHDW D++C++ILK+CKEA+ K GKV+I+D+V+D +S++ + RL +++DM
Sbjct: 246 IFMKCILHDWNDDECIQILKRCKEAL--PKVGKVIIVDVVIDMDSTHPYAKIRLTLDLDM 303
Query: 268 LVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
++ GGKER++EEW+ LF AAG++ +K+T I A++S+IE +P
Sbjct: 304 MLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 303 bits (776), Expect = 1e-79
Identities = 152/344 (44%), Positives = 221/344 (64%), Gaps = 6/344 (1%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRLF 977
D QA++W+++YGFAESL LK A++L I D + HG+PMTLSELA LP+ V+ DRL+
Sbjct: 10 DQATQAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLY 69
Query: 976 RIMRYLVHMGLF----DLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYE--MDAWHH 815
R+MRYLVHM LF D + KY+L P + LIKG +KS+ S LL + M WHH
Sbjct: 70 RVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHH 129
Query: 814 LSAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXX 635
L + ++K G +Y + + N++ ++ M + +V+ ALV C
Sbjct: 130 LKEGLVNNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNEC--GNIFQ 187
Query: 634 XXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPET 455
A +AV+KAFP IKC+++DLPHVI+ +P+ + +IEG+MF S+P
Sbjct: 188 GIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSA 247
Query: 454 DVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEM 275
D + MK +LHDW D++C++ILKKCKEA+ ++GGKV+I+DIVLD ES + + RL +++
Sbjct: 248 DAILMKCILHDWNDDECIEILKKCKEAV-PKEGGKVIIVDIVLDMESKHPYSKVRLTLDL 306
Query: 274 DMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
DM++ GKER+EEEW+KL AG++ YKIT I A++S+IE FP
Sbjct: 307 DMMLNTEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFP 350
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 298 bits (764), Expect = 3e-78
Identities = 152/348 (43%), Positives = 223/348 (64%), Gaps = 8/348 (2%)
Frame = -2
Query: 1162 EEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDR 983
+E++ +QA++W ++YGFA+SL LK A+QL + +I+ HG PMTLSEL+ LP VNQD
Sbjct: 6 KENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDA 65
Query: 982 LFRIMRYLVHMGLF--DLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYEMD---AWH 818
L+R++RYLVHM LF +D + +Y L P + L+KG DK + A+L + D WH
Sbjct: 66 LYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLG-AILTITDKDFMAPWH 124
Query: 817 HLSAAV--EGGV-TPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKA 647
+L + +G T +EK G + +Y A+ NQL ++ M + T ++ ALV+ C +
Sbjct: 125 YLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKEC--S 182
Query: 646 KXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVS 467
A R +AKAFP IKC V+DLPHVIA +P E+ I+GDMF
Sbjct: 183 SMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKY 242
Query: 466 VPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARL 287
+P D + MK +LHDW D++C++ILK+CK+A+ R GGKV+I+DI+LD +S + + RL
Sbjct: 243 IPNADAIMMKCILHDWDDKECIEILKRCKDAV-PRDGGKVIIIDIILDVKSEHPYTKMRL 301
Query: 286 GMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
+++DM++ GGKER+EEEW+KL AGY YKIT I A++S+IE +P
Sbjct: 302 TLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 293 bits (749), Expect = 2e-76
Identities = 150/348 (43%), Positives = 219/348 (62%), Gaps = 5/348 (1%)
Frame = -2
Query: 1171 EKMEEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVN 992
E ++++ +QA++W ++YGFAESL LKCA+QL + +I+ G MTLSEL+ LP VN
Sbjct: 2 EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61
Query: 991 QDRLFRIMRYLVHMGLFDL--VDSDKKYALNPASNLLIKGQDKSLAS--FALLQYYEMDA 824
+D L+R+MRYLVHM LF +D + +Y L P + L+KG DK + A+ M
Sbjct: 62 EDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAP 121
Query: 823 WHHLSAAVEG-GVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKA 647
WH+L + G T +EK G + Y A+ NQL ++AM + + ++ ALV+ C
Sbjct: 122 WHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKEC--G 179
Query: 646 KXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVS 467
A R +A AFP IKC V+DLPHVIA +P +EV + GDMF
Sbjct: 180 NIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKF 239
Query: 466 VPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARL 287
+P+ D + MK +LHDW D++C++ILK+CKEA+ KGGKV+I+DIVL+ +S + + RL
Sbjct: 240 IPKADAIMMKCILHDWDDKECIEILKRCKEAV-PVKGGKVIIVDIVLNVQSEHPYTKMRL 298
Query: 286 GMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
+++DM++ GGKER+EEEW+KL AGY +KIT I A++S+IE +P
Sbjct: 299 TLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346
>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum flavum]
Length = 346
Score = 284 bits (726), Expect = 7e-74
Identities = 145/347 (41%), Positives = 214/347 (61%), Gaps = 8/347 (2%)
Frame = -2
Query: 1159 EDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRL 980
ED+ +QA++W Y+YGFA+SL LKCA+QL + +I+ HG PMTLSEL+ LP VNQD L
Sbjct: 2 EDLSSQAKLWNYIYGFADSLVLKCAVQLDLANIIHDHGTPMTLSELSSRLPSQPVNQDAL 61
Query: 979 FRIMRYLVHMGLF--DLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYE--MDAWHHL 812
+R+MRYLVH+ LF +D + +Y L P + L+KG D + LL M +H++
Sbjct: 62 YRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMVDTDFMAPYHYI 121
Query: 811 SAAV---EGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKX 641
+ +T +EK G + EY A+ NQL ++ M + + + L++ C +
Sbjct: 122 KDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMSGLMKDC--SST 179
Query: 640 XXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVP 461
+ +AKAFP IK V+DLPHVIA AP E+ +EGDMF +P
Sbjct: 180 FEGLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEINFVEGDMFKYIP 239
Query: 460 ETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGM 281
D + MK VLHDW D +C++ILK+CK+A+ R GGKV+I+D+VL+ +S + + RL +
Sbjct: 240 NADAILMKGVLHDWDDNECIEILKRCKDAV-PRDGGKVIIIDVVLNVKSEHPYTKMRLTL 298
Query: 280 EMDMLVTVGGKERSEEEWQKL-FKAAGYSRYKITPIVAIESIIELFP 143
++DM++ GKER+EE+W+KL + AGY YKIT I A++S+IE +P
Sbjct: 299 DLDMMLNNKGKERTEEDWKKLIYDDAGYKGYKITQISALQSVIEAYP 345
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
flavum subsp. glaucum]
Length = 348
Score = 281 bits (719), Expect = 5e-73
Identities = 145/341 (42%), Positives = 213/341 (62%), Gaps = 3/341 (0%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRLF 977
D+KAQA VW+ +YGFA+SL L+CA++LGI DI+ + +P+ L++LA LP+ VN D LF
Sbjct: 12 DIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPISNVNLDNLF 71
Query: 976 RIMRYLVHMGLFDLVDSDKKYALNPASNLLIKGQDKSLASFAL--LQYYEMDAWHHLSAA 803
RI+RYLV M L +D KYAL P + L+++ + +S+ L Q M WH +
Sbjct: 72 RILRYLVKMELLSYA-ADDKYALEPIAKLVLRNEKRSMVPMVLGMTQKDFMTPWHSMKDG 130
Query: 802 V-EGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXXXX 626
+ + GVT +EK G+ EY +QL ++ M T ++T +L+ G K
Sbjct: 131 LTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSKDM--FQSID 188
Query: 625 XXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETDVV 446
+A++ AFP IKC +FDLPHVIA + + +ERI GDMF +P +
Sbjct: 189 SLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKGMPSAQAI 248
Query: 445 FMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMDML 266
+K +LHDW DED +KILK+C++A+ + GGKV+I+D+ LD +S +E RL +++DML
Sbjct: 249 LLKLILHDWNDEDSIKILKQCRKAV-PKDGGKVIIVDVALDEDSDHELSSTRLILDIDML 307
Query: 265 VTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
V GGKER++E+W+KL K AG+S KI I AI+S+IE+FP
Sbjct: 308 VNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348
>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
gi|9711916|dbj|BAB08005.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
4'-O-methyltransferase [Coptis japonica]
Length = 350
Score = 281 bits (718), Expect = 6e-73
Identities = 146/343 (42%), Positives = 210/343 (61%), Gaps = 5/343 (1%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRLF 977
D+KAQA VW+ +YGFA+SL L+CA++LGI DI+ + +PM L++LA LP+ VN D L+
Sbjct: 11 DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLY 70
Query: 976 RIMRYLVHMGLFDLVDSD---KKYALNPASNLLIKGQDKSLASFAL--LQYYEMDAWHHL 812
RI+RYLV M + + SD KKYAL P + LL + +S+ L Q M WH +
Sbjct: 71 RILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSM 130
Query: 811 SAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXX 632
+ T +EK G+ EY +QL ++ M T ++T +L+ G +
Sbjct: 131 KDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDM--FQG 188
Query: 631 XXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETD 452
+A++ AFP IKC +FDLPHVIA + + +ERI GDMF SVP
Sbjct: 189 IDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSAQ 248
Query: 451 VVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMD 272
+ +K +LHDW DED +KILK+C+ A+ + GGKV+I+D+ LD ES +E RL +++D
Sbjct: 249 AIILKLILHDWNDEDSIKILKQCRNAV-PKDGGKVIIVDVALDEESDHELSSTRLILDID 307
Query: 271 MLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
MLV GGKER++E W+K+ K+AG+S KI I AI+S+IE+FP
Sbjct: 308 MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350
>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase [Coptis chinensis]
Length = 350
Score = 280 bits (716), Expect = 1e-72
Identities = 146/343 (42%), Positives = 210/343 (61%), Gaps = 5/343 (1%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRLF 977
D+KAQA VW+ +YGFA+SL L+CA++LGI DI+ + +PM L++LA LP+ VN D L+
Sbjct: 11 DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLPVSNVNCDNLY 70
Query: 976 RIMRYLVHMGLFDLVDSD---KKYALNPASNLLIKGQDKSLASFAL--LQYYEMDAWHHL 812
RI+RYLV M + + SD KKYAL P + LL + +S+ L Q M WH +
Sbjct: 71 RILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSM 130
Query: 811 SAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXX 632
+ T +EK G+ EY +QL ++ M T ++T +L+ G +
Sbjct: 131 KDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDM--FQG 188
Query: 631 XXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETD 452
+A++ AFP IKC +FDLPHVIA + + +ERI GDMF SVP
Sbjct: 189 IDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSAQ 248
Query: 451 VVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMD 272
+ +K +LHDW DED +KILK+C+ A+ + GGKV+I+D+ LD ES +E RL +++D
Sbjct: 249 AILLKLILHDWNDEDSIKILKQCRNAV-PKDGGKVIIVDVALDEESDHELSSTRLILDID 307
Query: 271 MLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
MLV GGKER++E W+K+ K+AG+S KI I AI+S+IE+FP
Sbjct: 308 MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350
>gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 357
Score = 278 bits (710), Expect = 5e-72
Identities = 145/355 (40%), Positives = 218/355 (61%), Gaps = 17/355 (4%)
Frame = -2
Query: 1162 EEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPT-VNQD 986
+ D + QA +W+ +YGFAESL LKCA+QL I + + HG+PM+LSELA LP+ VN D
Sbjct: 6 QTDQENQAIIWKQIYGFAESLVLKCAVQLEIAETIHNHGKPMSLSELASKLPIDQPVNID 65
Query: 985 RLFRIMRYLVHMGLFDLVDS-----------DKKYALNPASNLLIKGQDKSLASFALLQY 839
RL+R+MR+LVHM LF+ + + KY+L P + LI+G ++S+ + L
Sbjct: 66 RLYRVMRFLVHMKLFNKENISILSGETAQVVEDKYSLAPPAKYLIRGSEQSMVPWVLGIT 125
Query: 838 YE--MDAWHHLSAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALV 665
E + WH L + G +EK G + +Y +++ NQ+ ++ M + +VT ALV
Sbjct: 126 DEDLVAPWHWLKEGITGNCNVFEKVLGKSHADYMSENPKKNQMFNELMACDSRLVTSALV 185
Query: 664 EGCKKAKXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIE 485
C+ A A++KAFP I+C +FDLPHVI +PE V +
Sbjct: 186 NECRSV-FSDGINTLVDVGGGTGTAVSAISKAFPNIQCTLFDLPHVIGDSPEIPNVTKTS 244
Query: 484 GDMFVSVPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYE 305
GDMF S+P D +FMK++LHDW D++CV+ILK+CKE + GGKV+++++V+D E ++
Sbjct: 245 GDMFQSIPSADAIFMKNILHDWNDDECVQILKRCKEVVS--VGGKVILVEMVMDTELAHP 302
Query: 304 FKGARLGMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPI---VAIESIIEL 149
+ RL +++DMLV GGKE +EEEW+KL AAG++ YKIT + A +S+IE+
Sbjct: 303 YSKLRLTLDLDMLVNNGGKEGTEEEWKKLIYAAGFTSYKITQMSAGFAAQSVIEV 357
>gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum]
gi|388571230|gb|AFK73711.1| O-methyltransferase [Papaver
somniferum]
Length = 339
Score = 275 bits (703), Expect = 3e-71
Identities = 142/341 (41%), Positives = 214/341 (62%), Gaps = 3/341 (0%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRLF 977
D + QA++W+ ++GFAESL LKCA+QL I + L + +PM+LSELA LP VN+DRL+
Sbjct: 8 DQENQAKIWKQIFGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPAQPVNEDRLY 67
Query: 976 RIMRYLVHMGLFDLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYEMD---AWHHLSA 806
RI+ +LVHM LF+ + +KY+L P + L+KG +KS+ ++L + D W+HL
Sbjct: 68 RILHFLVHMKLFNKDATTQKYSLAPPAKYLLKGWEKSMVP-SILSVTDKDFTAPWNHLGD 126
Query: 805 AVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXXXXXX 626
+ G +EK G R Y ++ +QL ++ M T + ALV CK
Sbjct: 127 GLTGNCNAFEKALGKGIRVYMRENPEKDQLFNEGMACDTRLFASALVNECKSI-FSDGIN 185
Query: 625 XXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPETDVV 446
A +A++KAFP IKC + DLP V ++ +I D+F SVP D +
Sbjct: 186 TLAGVGRGTGTAVKAISKAFPDIKCTIHDLPEV------TSKNSKIPRDVFKSVPSADAI 239
Query: 445 FMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGMEMDML 266
FMKS+LH+W DE+C++ILK+CKEAI KGGKV+I D+V+D +S++ + +RL M++ M+
Sbjct: 240 FMKSILHEWNDEECIQILKRCKEAI--PKGGKVIIADVVIDMDSTHPYSKSRLAMDLAMM 297
Query: 265 VTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
+ GGKER+EE+W+KL AAG++ KIT + A++S+IE +P
Sbjct: 298 LHTGGKERTEEDWKKLIDAAGFASCKITKLSALQSVIEAYP 338
>ref|XP_006858228.1| hypothetical protein AMTR_s00062p00190100 [Amborella trichopoda]
gi|548862331|gb|ERN19695.1| hypothetical protein
AMTR_s00062p00190100 [Amborella trichopoda]
Length = 353
Score = 273 bits (698), Expect = 1e-70
Identities = 141/345 (40%), Positives = 217/345 (62%), Gaps = 5/345 (1%)
Frame = -2
Query: 1162 EEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDR 983
+E+++AQA +W +++G A+++ L CA++LG+ DI+ HGRP+TL LA LP+ VN DR
Sbjct: 13 KEEVEAQAVIWEHMFGMAKTMALNCAVRLGLADIIHAHGRPITLFHLASLLPIQPVNPDR 72
Query: 982 LFRIMRYLVHMGLF----DLVDSDKKYALNPASNLLIKGQDKSLASFALLQYYEMDAWHH 815
L R+MR+LVH LF L ++ Y L P+S LL++ +KS++ FA + + W
Sbjct: 73 LHRLMRFLVHTHLFAKTTGLNGDEETYWLTPSSQLLLRHAEKSMSGFARVHEMTVSPWLS 132
Query: 814 LSAAVEGGV-TPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXX 638
L +E GV + +EK +G+ EY A N+ ++AM +V A+V GC++
Sbjct: 133 LGPCLEEGVASAFEKTYGMSRWEYSASHPDENRAFNEAMACLARLVMPAIVGGCERL--L 190
Query: 637 XXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPE 458
A A+AKAFP +K ++DLPHV+AT E V+ + GDMF ++P+
Sbjct: 191 QGARSVVDVGGGTGTAVGALAKAFPSVKFTLYDLPHVVATVGELNGVDTVGGDMFSAIPK 250
Query: 457 TDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGME 278
DVVF+ +LHDWGD++C++ILK+ ++AI + GKV+I+D+VL E + F+ AR+G++
Sbjct: 251 ADVVFLMRILHDWGDKECLEILKQIRKAI--PEEGKVIIVDVVLKEEEEHVFRHARMGLD 308
Query: 277 MDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
M M+V GGKER E EW KL AG+ +Y++ PI+A+ SII FP
Sbjct: 309 MVMMVQTGGKERDEGEWAKLLHDAGFKKYEVVPIMAVYSIIVAFP 353
>gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 1 [Papaver somniferum]
Length = 354
Score = 269 bits (688), Expect = 2e-69
Identities = 140/351 (39%), Positives = 210/351 (59%), Gaps = 7/351 (1%)
Frame = -2
Query: 1174 SEKMEEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTV 995
+E E D+K QAQ+W +YG+A+SL L+C +++GI DI+ + +TLSEL LPL V
Sbjct: 7 AETHEVDIKDQAQLWNIIYGYADSLVLRCTVEIGIADIIKNNNGSITLSELVSKLPLSNV 66
Query: 994 NQDRLFRIMRYLVHMGLFD----LVDSDKKYALNPASNLLIKGQDKSLAS--FALLQYYE 833
N D L+R++RYLVH+ + D+ Y+L P LL+K ++S+A L Q
Sbjct: 67 NSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILGLSQKDF 126
Query: 832 MDAWHHLSAAV-EGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGC 656
+ W+ + + G T +EK G+D +Y + +QL + T ++T+ L+ C
Sbjct: 127 LFVWNFVKEGLGTGSTTAFEKAMGMDMWKYLEVNPNQSQLFDEGQAGETRLLTKTLLVDC 186
Query: 655 KKAKXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDM 476
+ +A+ +AFP IKC ++DLPHVIA + + + ++ GDM
Sbjct: 187 RDT--FQGMDSLVDVGGGNGTTIKAIHEAFPHIKCTLYDLPHVIANSDDHPNILKVPGDM 244
Query: 475 FVSVPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKG 296
F+SVP V+ +K VLHDW DE CV ILKKCKEAI ++ GKV+I+D+ L+ ES +E
Sbjct: 245 FMSVPSAQVLLLKCVLHDWTDEHCVNILKKCKEAI-PKETGKVIIVDVALEEESEHELTK 303
Query: 295 ARLGMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
ARL +++DMLV GG+ER+ E+W+ L K AG+ +KI PI AI+S+IE FP
Sbjct: 304 ARLILDIDMLVNTGGRERTAEDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 354
>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-
O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 269 bits (688), Expect = 2e-69
Identities = 136/352 (38%), Positives = 215/352 (61%), Gaps = 7/352 (1%)
Frame = -2
Query: 1177 ASEKMEEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPT 998
A+ E +K QAQ+W +YGFA+SL L+CA+++GI DI+ + +TL++LA LP+
Sbjct: 9 AAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITN 68
Query: 997 VNQDRLFRIMRYLVHMGLFDLVDS----DKKYALNPASNLLIKGQDKSLASFAL--LQYY 836
V+ D L+R++RYLVH+ + + +K Y+L P LL++ ++S+ L Q
Sbjct: 69 VSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKD 128
Query: 835 EMDAWHHLSAAV-EGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEG 659
M +WH + + G T +EK G+D +Y + +QL ++ M T ++T+ L+E
Sbjct: 129 FMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIED 188
Query: 658 CKKAKXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGD 479
C+ +A+ +AFP IKC ++DLPHV+A + + +E++ GD
Sbjct: 189 CRDT--FQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGD 246
Query: 478 MFVSVPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFK 299
MF SVP + +K +LHDW DE+CV ILKKCKEAI ++ GKV+I+D+ L+ ES++E
Sbjct: 247 MFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAI-PKETGKVIIVDVALEEESNHELT 305
Query: 298 GARLGMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
RL +++DMLV GG+ER+ ++W+ L K AG+ +KI PI AI+S+IE FP
Sbjct: 306 KTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
californica]
Length = 353
Score = 269 bits (687), Expect = 2e-69
Identities = 137/349 (39%), Positives = 207/349 (59%), Gaps = 9/349 (2%)
Frame = -2
Query: 1162 EEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDR 983
E D+KAQA +W +YGFA+SL L+ A++LGI DI+ + +T+SELA LP+ VN D
Sbjct: 8 EVDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNSDN 67
Query: 982 LFRIMRYLVHMGLFDLVDSD-------KKYALNPASNLLIKGQDKSLASFAL--LQYYEM 830
L+R++RYLVHMG+ S K Y+L P +LL+K ++++ L Q M
Sbjct: 68 LYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQDFM 127
Query: 829 DAWHHLSAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKK 650
WH++ + G T +EK G+ EY L + M T ++T+ L+E CK
Sbjct: 128 IPWHYIKEGLGEGSTAFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESCKD 187
Query: 649 AKXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFV 470
+A+++AFP IKC+++DLPHV+A + + +E+I GD+F
Sbjct: 188 T--FEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIFK 245
Query: 469 SVPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGAR 290
+P + +K +LHDW DED VKILKKC+EA+ + G+V+I+D+ L+ ES + R
Sbjct: 246 FIPNAQAILLKLILHDWSDEDSVKILKKCREAV-PQDTGRVIIVDVALEEESEHPLTKTR 304
Query: 289 LGMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
L +++DMLV GG+ERSE++W KL K AG+ +KI I A++S+IE FP
Sbjct: 305 LVLDVDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353
>ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase 9 [Vitis vinifera]
Length = 354
Score = 263 bits (671), Expect = 2e-67
Identities = 143/347 (41%), Positives = 212/347 (61%), Gaps = 8/347 (2%)
Frame = -2
Query: 1159 EDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRL 980
E +AQ+ ++++V+ F +S++LKCA+QLGIPD + H +P+TL ELA ++ +P RL
Sbjct: 11 ELFEAQSFIYKHVFSFMDSMSLKCAVQLGIPDAIHNHNQPITLPELASAIQVPPEKTSRL 70
Query: 979 FRIMRYLVHMGLFDLVDSDKK---YALNPASNLLIKGQDKSLASFAL--LQYYEMDAWHH 815
++MR LVH G F + D+ Y L P S LL+KG SLA L L + WH
Sbjct: 71 HQLMRLLVHSGFFAMQKVDENQEGYVLTPPSRLLVKGNATSLAPIVLGMLDPVLVTPWHF 130
Query: 814 LSAAVEGG-VTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXX 638
L + ++G +T +E HG+D Y ++ L+ + M + + M++ A+ E CK+
Sbjct: 131 LGSWLQGSSLTAFEAAHGMDLWNYGNQNPEFFSLIGEIMATDSRMMSLAIRE-CKEI--F 187
Query: 637 XXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPE 458
AR + +AFP +KC V DLP V+A P+ ++ + GDMF S+P
Sbjct: 188 EGLSSLVDVGGGTGTMARGICEAFPHLKCTVLDLPQVVANLPKSENLDYVGGDMFQSIPS 247
Query: 457 TDVVFMKSVLHDWGDEDCVKILKKCKEAI-GGRKGGKVVILDIVL-DAESSYEFKGARLG 284
D +F+KSVLH+WGDEDCVKILK+C+EAI +GGKV+I+D+VL + + +E +L
Sbjct: 248 ADAIFIKSVLHNWGDEDCVKILKRCREAIPSSAEGGKVIIIDLVLSNKKDEHELAKTKLF 307
Query: 283 MEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
+M M+V V GKER EEEW+KLF AG+S YKITP + S+IE++P
Sbjct: 308 NDMMMMVLVAGKERCEEEWEKLFLEAGFSHYKITPRFGVLSLIEVYP 354
>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT; AltName:
Full=Tetrahydrocolumbamine 2-O-methyltransferase
gi|24210466|dbj|BAC22084.1| columbamine
O-methyltransferase [Coptis japonica]
Length = 351
Score = 262 bits (669), Expect = 3e-67
Identities = 140/342 (40%), Positives = 211/342 (61%), Gaps = 6/342 (1%)
Frame = -2
Query: 1162 EEDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDR 983
++++KAQAQVW++++GFAE++ L+ + LGIPDI+ +G P+TLS+L LPL + + DR
Sbjct: 10 DDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKSTSIDR 68
Query: 982 LFRIMRYLVHMGLF----DLVDSDKKYALNPASNLLIKGQDKSLASFALLQYY--EMDAW 821
MRYLVHM LF D + + KY L PAS LL+ G KSLA + +LQ + E W
Sbjct: 69 FHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVW 128
Query: 820 HHLSAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKX 641
H+ ++G WE + E D N++L+DAMTSH++ + ALV G K
Sbjct: 129 SHVINVLDGKKPYWES-NDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVSGLMKENV 187
Query: 640 XXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVP 461
V A+ + AFP +KC+V DL HVI + +++ + GDMF S+P
Sbjct: 188 LDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMFTSIP 247
Query: 460 ETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKGARLGM 281
D + +KS LH++ D+DC+KIL KEA+ GGKV++++IV+D E+ F ARL M
Sbjct: 248 NADAILLKSTLHNYEDDDCIKILNIAKEALPS-TGGKVILVEIVVDTENLPLFTSARLSM 306
Query: 280 EMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESII 155
MDM++ + GKER+++EW+ L + A ++ +++ PI+AIESII
Sbjct: 307 GMDMML-MSGKERTKKEWEDLLRKANFTSHQVIPIMAIESII 347
>gb|ACN88562.1| norreticuline-7-O-methyltransferase [Papaver somniferum]
Length = 357
Score = 262 bits (669), Expect = 3e-67
Identities = 140/353 (39%), Positives = 209/353 (59%), Gaps = 16/353 (4%)
Frame = -2
Query: 1156 DMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPT-VNQDRL 980
D + QA +W+ +YGF+ESL LKCA+Q I + + HG PM++ ELA LP+ VN DRL
Sbjct: 8 DQENQAIIWKQIYGFSESLLLKCAVQCEIAETIHNHGTPMSILELAAKLPIDQPVNIDRL 67
Query: 979 FRIMRYLVHMGLFD----------LVDSDKKYALNPASNLLIKGQDKSLASFAL--LQYY 836
+R+MRYLVH LF+ V +KY L P + LI+G +S+ L +
Sbjct: 68 YRVMRYLVHQKLFNKEVISTLNGGTVQVTEKYWLAPPAKYLIRGSQQSMVPSVLGIIDED 127
Query: 835 EMDAWHHLSAAVEGGVTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGC 656
WH L ++ G +E G Y +++ NQ+ + AM + +VT LV C
Sbjct: 128 MFAPWHILKDSLTGECNIFETALGKSISVYMSENPEMNQISNGAMAFDSGLVTSHLVNEC 187
Query: 655 KKAKXXXXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDM 476
K A RA++KAFP IKC +FDLPHVIA +PE + ++ GDM
Sbjct: 188 KSV-FGDEIKTLVDVGGGTGTALRAISKAFPNIKCTLFDLPHVIADSPEIPTITKVSGDM 246
Query: 475 FVSVPETDVVFMKSVLHDWGDEDCVKILKKCKEAIGGRKGGKVVILDIVLDAESSYEFKG 296
F S+P D +FMK++LHDW D++C++ILK+CK+ + GGK++++++VLD +S + +
Sbjct: 247 FKSIPSADAIFMKNILHDWNDDECIQILKRCKDVVSA--GGKLIMVEMVLDEDSFHPYSK 304
Query: 295 ARLGMEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPI---VAIESIIELF 146
RL ++DM+V GGKER+E+EW+KLF AAG++ K T + A +SIIE++
Sbjct: 305 LRLTSDIDMMVNNGGKERTEKEWEKLFDAAGFASCKFTQMSVGFAAQSIIEVY 357
>emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera]
Length = 354
Score = 261 bits (667), Expect = 5e-67
Identities = 143/347 (41%), Positives = 212/347 (61%), Gaps = 8/347 (2%)
Frame = -2
Query: 1159 EDMKAQAQVWRYVYGFAESLTLKCAIQLGIPDILLQHGRPMTLSELADSLPLPTVNQDRL 980
E +AQ+ ++++V+ F +S++LKCA+QLGIPD + H +P+TL ELA ++ +P RL
Sbjct: 11 ELFEAQSFIYKHVFSFMDSMSLKCAVQLGIPDAIHNHNQPITLPELASAIQVPPEKTSRL 70
Query: 979 FRIMRYLVHMGLFDLVDSDKK---YALNPASNLLIKGQDKSLASFAL--LQYYEMDAWHH 815
++MR LVH G F + D+ Y L P S LL+KG SLA L L + WH
Sbjct: 71 HQLMRLLVHSGFFAMQKVDENQEGYVLTPPSRLLVKGNATSLAPIVLGMLDPVLVTPWHF 130
Query: 814 LSAAVEGG-VTPWEKCHGVDYREYFAKDSVANQLLSDAMTSHTSMVTEALVEGCKKAKXX 638
L + ++G +T +E HG+D Y ++ + + M + + M++ A+ E CK+
Sbjct: 131 LGSWLQGSSLTAFEAAHGMDLWNYGNQNPEXFSXIGEIMATDSRMMSLAIRE-CKEI--F 187
Query: 637 XXXXXXXXXXXXXXVAARAVAKAFPGIKCAVFDLPHVIATAPECAEVERIEGDMFVSVPE 458
AR + +AFP +KC V DLP V+A P+ ++ + GDMF S+P
Sbjct: 188 EGXSSLVDVGGGTGTMARGICEAFPHLKCTVLDLPQVVANLPKSENLDYVGGDMFQSIPS 247
Query: 457 TDVVFMKSVLHDWGDEDCVKILKKCKEAI-GGRKGGKVVILDIVL-DAESSYEFKGARLG 284
D +F+KSVLH+WGDEDCVKILK+C+EAI +GGKV+I+D+VL + + +E +L
Sbjct: 248 ADAIFIKSVLHNWGDEDCVKILKRCREAIPSSAEGGKVIIIDLVLSNKKDEHELAKTKLF 307
Query: 283 MEMDMLVTVGGKERSEEEWQKLFKAAGYSRYKITPIVAIESIIELFP 143
+M M+V V GKER EEEW+KLF AG+S YKITP + S+IE++P
Sbjct: 308 NDMMMMVLVAGKERCEEEWEKLFLEAGFSHYKITPRFGVLSLIEVYP 354