BLASTX nr result
ID: Sinomenium22_contig00017178
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium22_contig00017178
(1572 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica] 213 2e-68
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp.... 218 1e-67
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera] 208 1e-67
ref|XP_002264336.1| PREDICTED: reticuline oxidase-like [Vitis vi... 213 7e-67
ref|XP_004292414.1| PREDICTED: reticuline oxidase-like [Fragaria... 215 5e-65
ref|XP_003622343.1| Reticuline oxidase [Medicago truncatula] gi|... 204 2e-64
ref|XP_003555533.1| PREDICTED: reticuline oxidase-like [Glycine ... 210 2e-64
gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver so... 203 6e-64
sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName... 207 4e-63
pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 207 4e-63
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 207 4e-63
pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 207 4e-63
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana] 196 3e-62
pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ... 204 5e-62
pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ... 204 5e-62
ref|XP_004495433.1| PREDICTED: reticuline oxidase-like [Cicer ar... 205 6e-62
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ... 203 6e-62
pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a ... 207 6e-62
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana] 193 2e-61
ref|XP_003634619.1| PREDICTED: LOW QUALITY PROTEIN: reticuline o... 210 2e-61
>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
Length = 533
Score = 213 bits (541), Expect(2) = 2e-68
Identities = 113/214 (52%), Positives = 149/214 (69%), Gaps = 1/214 (0%)
Frame = -2
Query: 935 NQPFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSG 756
+ PFV+ID+M LN V +DL+++TAW E GATLGE+YHA+G SS+ LGF AG+C V S G
Sbjct: 114 DSPFVIIDMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGFSAGYCPTVGSGG 173
Query: 755 HIXXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVY 576
HI MSRKYGLA+DNVVD ILI A+G D +SMGEDVFWAIRGGG WG VY
Sbjct: 174 HISGGGFGM-MSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRGGGGGVWGVVY 232
Query: 575 SWKIRLVDVPETVTAPRVTRHGSK-SEAAKLLNKWQWVALDLEDEFTLMASVIVKSETSI 399
+WK++L+ VP+ VT ++T+H S+ EA+KLL KWQ VA +L+D+F+L + +
Sbjct: 233 AWKLQLLPVPKHVTVFKLTKHTSEIDEASKLLYKWQLVAPNLDDDFSLAVLNGAEKDGFW 292
Query: 398 LTVFQGLYHGPRSSAVASITELFLNWMLLQVKAR 297
LT F GLY GP+ AV+S+ + F LL + +
Sbjct: 293 LT-FLGLYLGPKEVAVSSMHQKFPELNLLSEECK 325
Score = 75.5 bits (184), Expect(2) = 2e-68
Identities = 40/97 (41%), Positives = 59/97 (60%), Gaps = 4/97 (4%)
Frame = -3
Query: 1213 SITSGEDDFNFFSCISSRGV-NYITPY--TQAYHHIFSLAADNLRIT-SAYVKPVAIILP 1046
++T + + + SC+ GV NY TP+ Y + ++ N T S + +P IILP
Sbjct: 18 NLTCSQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQNQIFTRSTFPRPSVIILP 77
Query: 1045 ETKEQLPATISCCRKATWAFTYRSGGHSLEGLSYSAE 935
E+K+QL TISCCR+ +W RSGGHS EGLS++A+
Sbjct: 78 ESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTAD 114
Score = 72.8 bits (177), Expect = 4e-10
Identities = 31/72 (43%), Positives = 47/72 (65%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
++W+H ++YM ++ PR Y N V+ D G +D +ISA + +E+ R+WGEKYFL+
Sbjct: 435 INWLHQFYDYMGQFVGNNPRVGYVNHVDFDFGTIDWTNSSISASKAIEIARTWGEKYFLS 494
Query: 57 N*NRLVRAKAAI 22
N +RLV AK I
Sbjct: 495 NYDRLVGAKTLI 506
>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
Length = 535
Score = 218 bits (556), Expect(2) = 1e-67
Identities = 111/209 (53%), Positives = 145/209 (69%), Gaps = 2/209 (0%)
Frame = -2
Query: 935 NQPFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSG 756
+ PFV+IDLM LN + +DL+++TAW+E GATLGE+YHA+G SS + F AG+C S G
Sbjct: 111 DNPFVIIDLMNLNGISIDLDTQTAWVESGATLGEIYHAIGKSSGTMAFSAGYCPTGGSGG 170
Query: 755 HIXXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVY 576
HI MSRKYGLA+DNVVD +L+DA+G LD SMGEDVFWAIRGGG WGAVY
Sbjct: 171 HIAPGGFGM-MSRKYGLAADNVVDALLVDANGVVLDRESMGEDVFWAIRGGGGGVWGAVY 229
Query: 575 SWKIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVIVKSETSIL 396
+WK++LV VP+ VT R+ +H +A+KLL+KWQ VA LED+F+L ++SI
Sbjct: 230 AWKLQLVPVPKNVTIFRLMKHSEVEDASKLLHKWQLVAPKLEDDFSLAVLAGTNKDSSIW 289
Query: 395 TVFQGLYHGPRSSAVASITELF--LNWML 315
F GLY GP+ A +S+ + F LN +L
Sbjct: 290 LTFLGLYLGPKELASSSMHKKFPELNLLL 318
Score = 67.4 bits (163), Expect(2) = 1e-67
Identities = 35/87 (40%), Positives = 51/87 (58%), Gaps = 6/87 (6%)
Frame = -3
Query: 1177 SCISSRGVNYITPY-----TQAYHHIFSLAADNLRIT-SAYVKPVAIILPETKEQLPATI 1016
SC++ GV+ T + + AYH + ++ N T S + +P IILPE+ +QL I
Sbjct: 25 SCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRSTFPQPRVIILPESMDQLANVI 84
Query: 1015 SCCRKATWAFTYRSGGHSLEGLSYSAE 935
SCC + +W RSGGHS EGLS+ A+
Sbjct: 85 SCCTRGSWTIRLRSGGHSYEGLSHIAD 111
Score = 74.3 bits (181), Expect = 1e-10
Identities = 33/72 (45%), Positives = 48/72 (66%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+ W+H + +YM ++S PR Y N V+LDLG +D IS+ +E+ R+WGEKYF++
Sbjct: 432 IHWLHQLFDYMGKFVSNNPRVGYVNHVDLDLGRIDWVNKTISSGRAIEIARTWGEKYFMS 491
Query: 57 N*NRLVRAKAAI 22
N +RLVRAK I
Sbjct: 492 NYDRLVRAKTMI 503
>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
Length = 536
Score = 208 bits (530), Expect(3) = 1e-67
Identities = 107/202 (52%), Positives = 147/202 (72%), Gaps = 2/202 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV+IDLM LN++ +DLESKTAW+E GATLGE+Y A+ +S+ LGF G+C V S GHI
Sbjct: 112 PFVVIDLMNLNRISIDLESKTAWVESGATLGEIYCAISEASDTLGFSGGYCPTVGSGGHI 171
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNV+D +++DA+G LD SMGEDVFWAIRGGG WGA+Y+W
Sbjct: 172 SGGGFGM-MSRKYGLAADNVIDALIVDANGAVLDRSSMGEDVFWAIRGGGGGVWGAIYAW 230
Query: 569 KIRLVDVPETVTAPRVTRH-GSKSEAAKLLNKWQWVALDLEDEFTLMASVIVKSETS-IL 396
K++L+ VP+ VT ++ ++ + EA+K+L+KWQ VA LED+FTL SV+ ++T+ I
Sbjct: 231 KLQLLPVPKQVTVFKLMKNFDNIEEASKMLHKWQVVAPALEDDFTL--SVLAGADTNGIW 288
Query: 395 TVFQGLYHGPRSSAVASITELF 330
F GLY GP+ A++S+ + F
Sbjct: 289 FSFLGLYLGPKELAISSVDQNF 310
Score = 70.9 bits (172), Expect(3) = 1e-67
Identities = 38/98 (38%), Positives = 55/98 (56%), Gaps = 5/98 (5%)
Frame = -3
Query: 1213 SITSGEDDFNFFSCISSRGVNYITPYTQA----YHHIFSLAADNLRITSA-YVKPVAIIL 1049
+IT D N SC++S GV+ T + + YH + ++ N T Y +P IIL
Sbjct: 13 TITCTLGDVNLSSCLTSNGVSNFTALSTSSDSDYHRLLYVSMQNQIFTRPKYPRPSMIIL 72
Query: 1048 PETKEQLPATISCCRKATWAFTYRSGGHSLEGLSYSAE 935
P++KE+L A++ C + W RSGGHS EGLSY A+
Sbjct: 73 PQSKEELAASVVCSNRGLWTIRLRSGGHSYEGLSYVAD 110
Score = 27.3 bits (59), Expect(3) = 1e-67
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -1
Query: 333 FLELDVVASESKEMKWVESTA 271
F EL++V + KEM WVES A
Sbjct: 310 FPELNLVMEDCKEMSWVESFA 330
Score = 73.6 bits (179), Expect = 2e-10
Identities = 36/72 (50%), Positives = 46/72 (63%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+ W+HG + YM +L PR AY N V+LDLG +D I A +E+ R+WGEKYFL+
Sbjct: 431 IGWLHGFYNYMGQFLPSDPRIAYVNHVDLDLGRLDWTNSTI-ASNAIEIARTWGEKYFLS 489
Query: 57 N*NRLVRAKAAI 22
N RLVRAK I
Sbjct: 490 NYERLVRAKTLI 501
>ref|XP_002264336.1| PREDICTED: reticuline oxidase-like [Vitis vinifera]
Length = 540
Score = 213 bits (543), Expect(3) = 7e-67
Identities = 110/210 (52%), Positives = 145/210 (69%), Gaps = 4/210 (1%)
Frame = -2
Query: 947 VLCRNQPFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAV 768
V+ PFV+ID+M LNQV+VDLES+TAW+EGGATLGE Y+AV +SN GF AG C V
Sbjct: 118 VVLDGNPFVIIDMMSLNQVLVDLESETAWVEGGATLGETYYAVAEASNVHGFSAGSCPTV 177
Query: 767 RSSGHIXXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSW 588
GHI +SRKYGLA+DNVVD +LIDADGR +D +MGEDVFWAIRGGG W
Sbjct: 178 GVGGHISGGGFGL-LSRKYGLAADNVVDALLIDADGRLVDREAMGEDVFWAIRGGGGGVW 236
Query: 587 GAVYSWKIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLM----ASVI 420
G VY+W+I+L+ VPE VT+ ++R G+K A+L++KWQ++A LE F L A ++
Sbjct: 237 GIVYAWRIKLLKVPEIVTSCIMSRTGTKLHVAELVHKWQFIAPRLEPSFYLSVFVGAGLL 296
Query: 419 VKSETSILTVFQGLYHGPRSSAVASITELF 330
ET + F+G Y G RS A++ + ++F
Sbjct: 297 GGKETGVSASFKGFYLGSRSKAMSILNQVF 326
Score = 63.5 bits (153), Expect(3) = 7e-67
Identities = 35/84 (41%), Positives = 49/84 (58%), Gaps = 7/84 (8%)
Frame = -3
Query: 1177 SCISSRGVNYIT--PYTQ----AYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPAT 1019
SC++ VN T P+ Q AY+ + + + NLR KPVAI+LP+++EQL T
Sbjct: 33 SCLTRHNVNNFTVLPHKQNESPAYYRLLNFSIQNLRYAVPTAPKPVAIVLPQSREQLVNT 92
Query: 1018 ISCCRKATWAFTYRSGGHSLEGLS 947
+SCCR+ + R GGHS EG S
Sbjct: 93 VSCCREGLFEIRVRCGGHSYEGTS 116
Score = 27.3 bits (59), Expect(3) = 7e-67
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 348 INN*AFLELDVVASESKEMKWVESTAF 268
I N F EL + E +EM W+ES A+
Sbjct: 321 ILNQVFPELGIEIEECREMSWIESIAY 347
Score = 81.6 bits (200), Expect = 9e-13
Identities = 38/72 (52%), Positives = 52/72 (72%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+DW+ G ++ MTP++S GPRAAY N ++LDLGVM+ + S+ + VE+ R+WGEKYFL
Sbjct: 448 IDWIRGFYKSMTPHVSWGPRAAYVNYMDLDLGVMEMVNSSFSSGDPVEIARAWGEKYFLN 507
Query: 57 N*NRLVRAKAAI 22
N RLVR K I
Sbjct: 508 NYERLVRVKTLI 519
>ref|XP_004292414.1| PREDICTED: reticuline oxidase-like [Fragaria vesca subsp. vesca]
Length = 552
Score = 215 bits (548), Expect(3) = 5e-65
Identities = 111/207 (53%), Positives = 141/207 (68%), Gaps = 7/207 (3%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV+ID+M LN V VDLE++ AW+EGGATLGE YHA+ +S+ GF AG C V + GHI
Sbjct: 123 PFVIIDMMNLNTVSVDLETEMAWVEGGATLGETYHAIAKASSVHGFSAGSCPTVGAGGHI 182
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
+SRKYGLA+DNVVD +LIDADGR LD R MGEDVFWAIRGGG WG VY+W
Sbjct: 183 AGGGFGL-LSRKYGLAADNVVDALLIDADGRLLDRRGMGEDVFWAIRGGGGGVWGIVYAW 241
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTL-------MASVIVKS 411
KI+L+ VP+TVT +R G+KS A L++KWQ VA LED+F + +
Sbjct: 242 KIQLLRVPQTVTVFVASRAGNKSHVANLVDKWQHVAPYLEDDFYISCFVGAGLPEAKTSG 301
Query: 410 ETSILTVFQGLYHGPRSSAVASITELF 330
T I F+G Y GPRSSA++++ ++F
Sbjct: 302 TTGISATFKGFYLGPRSSAMSTLDQVF 328
Score = 54.3 bits (129), Expect(3) = 5e-65
Identities = 30/84 (35%), Positives = 45/84 (53%), Gaps = 7/84 (8%)
Frame = -3
Query: 1177 SCISSRGVNYITPY------TQAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPAT 1019
SC++ +N T + + +Y + + + NLR V KP+AIILPE+ EQ+ +
Sbjct: 32 SCLTLHNINNFTTFPNTENDSDSYFKLLNFSIQNLRFAEPTVPKPIAIILPESVEQIINS 91
Query: 1018 ISCCRKATWAFTYRSGGHSLEGLS 947
+ C R+ A R GGHS EG S
Sbjct: 92 VYCSREVFLAIRVRCGGHSYEGTS 115
Score = 28.5 bits (62), Expect(3) = 5e-65
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -1
Query: 333 FLELDVVASESKEMKWVESTAF 268
F ++ +V + KEM W+EST F
Sbjct: 328 FPDIGIVKEDCKEMSWIESTVF 349
Score = 70.9 bits (172), Expect = 2e-09
Identities = 36/73 (49%), Positives = 48/73 (65%), Gaps = 3/73 (4%)
Frame = -1
Query: 231 WVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISAD---ELVELERSWGEKYFL 61
W+ ++ MTPY+S GPR AY N ++LDLGVM + + + + VE+ R WGEKYFL
Sbjct: 453 WIRRLYNSMTPYVSWGPRTAYINYIDLDLGVMRLVETRVPTEKDKDAVEIARVWGEKYFL 512
Query: 60 AN*NRLVRAKAAI 22
N +RLVRAK I
Sbjct: 513 NNYDRLVRAKTII 525
>ref|XP_003622343.1| Reticuline oxidase [Medicago truncatula]
gi|355497358|gb|AES78561.1| Reticuline oxidase [Medicago
truncatula]
Length = 548
Score = 204 bits (519), Expect(3) = 2e-64
Identities = 105/203 (51%), Positives = 138/203 (67%), Gaps = 4/203 (1%)
Frame = -2
Query: 926 FVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHIX 747
FV+ID+M LN V V++E+K AW+EGGATLGE Y+A+ +S+ GF AG C V GHI
Sbjct: 129 FVIIDMMNLNHVWVNMETKIAWVEGGATLGETYYAISQASDAYGFSAGSCPTVGVGGHIG 188
Query: 746 XXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSWK 567
+SRKYGLA+DNVVD +L+DADGR L+ +MGEDVFWAIRGGG WG VY+WK
Sbjct: 189 GGGFGL-LSRKYGLAADNVVDALLVDADGRVLERATMGEDVFWAIRGGGGGLWGIVYAWK 247
Query: 566 IRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLM----ASVIVKSETSI 399
I+L+ VP+ VT+ +R G+K+ AKL+NKWQ VA +LEDEF L A + +
Sbjct: 248 IQLLKVPQVVTSFIASRTGTKNHIAKLVNKWQHVAPNLEDEFYLSCFVGAGLPEAKRIGL 307
Query: 398 LTVFQGLYHGPRSSAVASITELF 330
T F+G Y GP S A++ + + F
Sbjct: 308 STTFKGFYLGPMSKAISILNQDF 330
Score = 60.5 bits (145), Expect(3) = 2e-64
Identities = 33/94 (35%), Positives = 49/94 (52%), Gaps = 10/94 (10%)
Frame = -3
Query: 1186 NFFSCISSRGVNYIT---------PYTQAYHHIFSLAADNLRITSAYV-KPVAIILPETK 1037
NF C+ + + T Y Y+ I + + NLR T + KP+AI+LP+T
Sbjct: 31 NFKFCLDNNKIKNFTVFPYKEHDQSYANDYYKILNFSVQNLRFTEPNIPKPIAIVLPKTL 90
Query: 1036 EQLPATISCCRKATWAFTYRSGGHSLEGLSYSAE 935
QL +++CCR+ + RSGGHS EG S A+
Sbjct: 91 VQLQNSVACCREFSLEIRVRSGGHSYEGTSSVAD 124
Score = 31.6 bits (70), Expect(3) = 2e-64
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -1
Query: 348 INN*AFLELDVVASESKEMKWVESTAF 268
I N F ELDVV E +EM W+ES F
Sbjct: 325 ILNQDFPELDVVDEECREMSWIESVVF 351
Score = 75.1 bits (183), Expect = 8e-11
Identities = 38/72 (52%), Positives = 50/72 (69%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+DW+ G + MTP++S GPRAAY N ++ DLGVM+ IS D+ + R WGEKYFL+
Sbjct: 453 IDWIRGFYSSMTPFVSYGPRAAYINYMDFDLGVME----LISFDDDLVKARVWGEKYFLS 508
Query: 57 N*NRLVRAKAAI 22
N +RLVRAK I
Sbjct: 509 NYDRLVRAKTLI 520
>ref|XP_003555533.1| PREDICTED: reticuline oxidase-like [Glycine max]
Length = 550
Score = 210 bits (535), Expect(2) = 2e-64
Identities = 104/204 (50%), Positives = 139/204 (68%), Gaps = 4/204 (1%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV+ID+M LN V VD+E++TAW+EGGATLGE Y+A+ SN GF G C V GHI
Sbjct: 127 PFVIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQESNEHGFSGGSCPTVGVGGHI 186
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
+SRKYGLA+DNVVD +L+DA+G+ D +MGEDVFWAIRGGG WG +Y+W
Sbjct: 187 GGGGFGL-LSRKYGLAADNVVDALLVDANGKLFDRETMGEDVFWAIRGGGGGLWGIIYAW 245
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLM----ASVIVKSETS 402
KI+++ VP+ VT+ V+R G+KS A L++KWQ+VA +LED+F L A + T
Sbjct: 246 KIQVLKVPQVVTSFTVSRTGTKSHVANLVHKWQYVAPNLEDDFYLSCLVGAGLPQAKTTG 305
Query: 401 ILTVFQGLYHGPRSSAVASITELF 330
+ T F G Y GPR+SA + + + F
Sbjct: 306 LSTTFNGFYLGPRASATSILNQAF 329
Score = 64.7 bits (156), Expect(2) = 2e-64
Identities = 38/103 (36%), Positives = 55/103 (53%), Gaps = 19/103 (18%)
Frame = -3
Query: 1186 NFFSCISSRGV----------NYIT-PYTQ-------AYHHIFSLAADNLRITSAYV-KP 1064
+ FSC S RG+ N+ T PY + +Y I + + NLR + KP
Sbjct: 21 SLFSCASLRGLASCLDNHNIKNFTTFPYKEHDHSSAYSYFKILNFSIQNLRFAEPVIPKP 80
Query: 1063 VAIILPETKEQLPATISCCRKATWAFTYRSGGHSLEGLSYSAE 935
+AI+LPE+ EQL +++CCR+ + R GGHS EG SY A+
Sbjct: 81 IAIVLPESLEQLQKSVACCREGSMEIRVRCGGHSYEGTSYVAD 123
Score = 76.6 bits (187), Expect = 3e-11
Identities = 37/72 (51%), Positives = 50/72 (69%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+DW+ G + MTP++S GPRAAY N ++ DLGVM+ + + + VE R WGEKYFL+
Sbjct: 452 VDWIRGFYAAMTPFVSWGPRAAYINYMDFDLGVMEGIGNGANMKDAVEHARVWGEKYFLS 511
Query: 57 N*NRLVRAKAAI 22
N +RLVRAK I
Sbjct: 512 NYDRLVRAKTLI 523
>gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver somniferum]
Length = 535
Score = 203 bits (516), Expect(3) = 6e-64
Identities = 105/200 (52%), Positives = 138/200 (69%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV++D+M LN++ +D+ES+TAW+E GATLGE+Y+A+ S+ LGF AG+C V S GHI
Sbjct: 120 PFVIVDMMNLNRISIDVESETAWVESGATLGELYYAIAQSTETLGFTAGWCPTVGSGGHI 179
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILID+ G LD MGEDVFWAIRGGG WGAVY+W
Sbjct: 180 SGGGFGM-MSRKYGLAADNVVDAILIDSTGSILDREKMGEDVFWAIRGGGGGVWGAVYAW 238
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVIVKSETSILTV 390
KI+L+ VPE +T RVT++ A+ LL+KWQ+VA +L+++FT+ V + L +
Sbjct: 239 KIKLLPVPEKLTVFRVTKNVGIEGASSLLHKWQYVADELDEDFTVSVLGGVNGNDAWL-M 297
Query: 389 FQGLYHGPRSSAVASITELF 330
F GLY G +A I E F
Sbjct: 298 FLGLYLGREDAAKTIIDEKF 317
Score = 63.9 bits (154), Expect(3) = 6e-64
Identities = 36/100 (36%), Positives = 54/100 (54%), Gaps = 6/100 (6%)
Frame = -3
Query: 1216 TSITSGEDDFNFFS-CISSRGV-NYITPYTQAYHHIFSLAADNLR----ITSAYVKPVAI 1055
T + G+ + N S C++S GV N+ T T F L +++ KP I
Sbjct: 19 TCVRGGDVNDNLLSSCLNSHGVHNFTTLSTDTNSDYFKLLHASIQNPLFAKPTVSKPSFI 78
Query: 1054 ILPETKEQLPATISCCRKATWAFTYRSGGHSLEGLSYSAE 935
++P +KE+L T+ CC + +W RSGGHS EGLSY+A+
Sbjct: 79 VMPGSKEELSRTVHCCTRESWTIRLRSGGHSYEGLSYTAD 118
Score = 27.3 bits (59), Expect(3) = 6e-64
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -1
Query: 333 FLELDVVASESKEMKWVESTAF 268
F EL V E +EM WV STAF
Sbjct: 317 FPELGSVEEEFQEMSWVASTAF 338
Score = 67.8 bits (164), Expect = 1e-08
Identities = 29/71 (40%), Positives = 48/71 (67%)
Frame = -1
Query: 234 DWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLAN 55
+W+ ++Y+ P++SKGPR Y N ++L++G +D ++ S VE+ R+WGE+YF +N
Sbjct: 439 EWLTKFYDYLEPFVSKGPRVGYVNHIDLEIGGID-WRNKSSTSNAVEIARNWGERYFSSN 497
Query: 54 *NRLVRAKAAI 22
RLV+AK I
Sbjct: 498 YERLVKAKTLI 508
>sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|239110|gb|AAB20352.1| (S)-reticuline:oxygen
oxidoreductase (methylene-bridge-forming) [Eschscholzia
californica] gi|2897944|gb|AAC39358.1| berberine bridge
enzyme [Eschscholzia californica]
Length = 538
Score = 207 bits (527), Expect(2) = 4e-63
Identities = 108/201 (53%), Positives = 144/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+C V + GHI
Sbjct: 116 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHI 175
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 176 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 234
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 235 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 292
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 293 TMLGFHFGLKTVAKSTFDLLF 313
Score = 63.2 bits (152), Expect(2) = 4e-63
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 26 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 85
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 86 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 114
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 434 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 492
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 493 NYERLIRAKTLI 504
>pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Reticuline gi|210060956|pdb|3D2H|A Chain A,
Structure Of Berberine Bridge Enzyme From Eschscholzia
Californica, Monoclinic Crystal Form
gi|210060957|pdb|3D2J|A Chain A, Structure Of Berberine
Bridge Enzyme From Eschscholzia Californica, Tetragonal
Crystal Form
Length = 538
Score = 207 bits (527), Expect(2) = 4e-63
Identities = 108/201 (53%), Positives = 144/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+C V + GHI
Sbjct: 116 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHI 175
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 176 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 234
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 235 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 292
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 293 TMLGFHFGLKTVAKSTFDLLF 313
Score = 63.2 bits (152), Expect(2) = 4e-63
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 26 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 85
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 86 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 114
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 434 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 492
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 493 NYERLIRAKTLI 504
>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
Dehydroscoulerine
Length = 519
Score = 207 bits (527), Expect(2) = 4e-63
Identities = 108/201 (53%), Positives = 144/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+C V + GHI
Sbjct: 97 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHI 156
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 157 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 215
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 216 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 273
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 274 TMLGFHFGLKTVAKSTFDLLF 294
Score = 63.2 bits (152), Expect(2) = 4e-63
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 7 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 66
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 67 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 95
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 415 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 473
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 474 NYERLIRAKTLI 485
>pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Scoulerine
Length = 495
Score = 207 bits (527), Expect(2) = 4e-63
Identities = 108/201 (53%), Positives = 144/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+C V + GHI
Sbjct: 91 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHI 150
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 151 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 209
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 210 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 267
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 268 TMLGFHFGLKTVAKSTFDLLF 288
Score = 63.2 bits (152), Expect(2) = 4e-63
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 89
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 409 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 467
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 468 NYERLIRAKTLI 479
>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
Length = 554
Score = 196 bits (498), Expect(2) = 3e-62
Identities = 100/201 (49%), Positives = 144/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV+IDLM LN++ +D++S+TAW+E GATLGE+Y+A+ ++ LGF AG+C V S GHI
Sbjct: 128 PFVLIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHI 187
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNV D ILID+ G LD + MGEDVFWA+RGGG WGA+Y+W
Sbjct: 188 SGGGFGM-MSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGGVWGAIYAW 246
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVIVKSE-TSILT 393
KI+L+ VP+ VT RVT++ + EA+ L++KWQ+VA +L+D+FT+ S++ + +
Sbjct: 247 KIKLLPVPKKVTVFRVTKNVNIEEASFLIHKWQYVADELDDDFTV--SILGGANGNEVWV 304
Query: 392 VFQGLYHGPRSSAVASITELF 330
+F GL+ G ++ A + I + F
Sbjct: 305 IFLGLHLGCKTVAKSIIDKKF 325
Score = 71.6 bits (174), Expect(2) = 3e-62
Identities = 38/89 (42%), Positives = 53/89 (59%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGV-NYITPYTQA---YHHIFSLAADN-LRITSAYVKPVAIILPETKEQLPA 1022
+ SC++S GV NY TP + + Y ++ L+ N L S KP I+LP KE+L
Sbjct: 38 DILSCLTSNGVHNYTTPSSDSNSDYLRLYHLSIQNPLFEKSTISKPSLIVLPRNKEELSN 97
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
T+ CC + +W RSGGHS EGLSY+A+
Sbjct: 98 TVRCCTRGSWTIRLRSGGHSYEGLSYTAD 126
Score = 75.1 bits (183), Expect = 8e-11
Identities = 34/71 (47%), Positives = 49/71 (69%)
Frame = -1
Query: 234 DWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLAN 55
DW+ I++YM ++SK PR Y N ++LDLG +D N S++ +E+ R+WGEKYFL+N
Sbjct: 447 DWLRNIYDYMEMFVSKNPRVGYVNHIDLDLGGIDWSDKN-SSNNAIEIARNWGEKYFLSN 505
Query: 54 *NRLVRAKAAI 22
RL+RAK I
Sbjct: 506 YERLIRAKTLI 516
>pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
Complex With (S)-Reticuline
Length = 497
Score = 204 bits (518), Expect(2) = 5e-62
Identities = 107/201 (53%), Positives = 143/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+ V + GHI
Sbjct: 91 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWAPTVGTGGHI 150
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 151 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 209
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 210 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 267
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 268 TMLGFHFGLKTVAKSTFDLLF 288
Score = 63.2 bits (152), Expect(2) = 5e-62
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 89
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 409 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 467
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 468 NYERLIRAKTLI 479
>pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant
Length = 495
Score = 204 bits (518), Expect(2) = 5e-62
Identities = 107/201 (53%), Positives = 143/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+ V + GHI
Sbjct: 91 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWAPTVGTGGHI 150
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 151 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 209
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 210 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 267
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 268 TMLGFHFGLKTVAKSTFDLLF 288
Score = 63.2 bits (152), Expect(2) = 5e-62
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 89
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 409 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 467
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 468 NYERLIRAKTLI 479
>ref|XP_004495433.1| PREDICTED: reticuline oxidase-like [Cicer arietinum]
Length = 546
Score = 205 bits (521), Expect(2) = 6e-62
Identities = 106/214 (49%), Positives = 147/214 (68%), Gaps = 4/214 (1%)
Frame = -2
Query: 926 FVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHIX 747
FV+ID+M LN V VD+E++TAW+EGGATLGE Y+A+ +S+ GF AG C V GHI
Sbjct: 129 FVIIDMMNLNHVWVDMETETAWVEGGATLGETYYAISQASDAHGFSAGSCPTVGVGGHIG 188
Query: 746 XXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSWK 567
+SRKYGLA+DNV+D +L+DA+GR LD +MGEDVFWAIRGGG WG VY+WK
Sbjct: 189 GGGFGL-LSRKYGLAADNVLDALLVDANGRLLDRETMGEDVFWAIRGGGGGLWGIVYAWK 247
Query: 566 IRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASV---IVKSET-SI 399
I+L+ VP+ VT V+R G+KS+ AKL+NKWQ VA +LE +F L V + +++T +
Sbjct: 248 IKLLKVPQIVTGFVVSRTGTKSDIAKLVNKWQHVAPNLEHDFYLSCFVGAGLPEAKTIGL 307
Query: 398 LTVFQGLYHGPRSSAVASITELFLNWMLLQVKAR 297
F+G Y GP+S A++ + F +++ + R
Sbjct: 308 SATFKGFYLGPKSKAISILNHAFPELGVIEEECR 341
Score = 61.6 bits (148), Expect(2) = 6e-62
Identities = 38/102 (37%), Positives = 57/102 (55%), Gaps = 10/102 (9%)
Frame = -3
Query: 1210 ITSGEDDFNFFSCISSRGVNYIT--PY-----TQAYHH--IFSLAADNLRITSAYV-KPV 1061
++S +DF F C+ + T PY + AY++ I + + NLR KP+
Sbjct: 25 LSSSLEDFTF--CLDDSKIKNFTAFPYKDRDQSSAYNYFKILNFSIQNLRFAEPTTPKPI 82
Query: 1060 AIILPETKEQLPATISCCRKATWAFTYRSGGHSLEGLSYSAE 935
AI+LPE+ EQL T++CCR+ ++ RSGGHS EG S A+
Sbjct: 83 AIVLPESLEQLQKTVACCREFSFEIRVRSGGHSYEGTSSVAD 124
Score = 71.2 bits (173), Expect = 1e-09
Identities = 37/72 (51%), Positives = 50/72 (69%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+DW+ G + MT ++S GPRAAY N ++ DLGVM+ I+ D +V+ R WGEKYFL+
Sbjct: 453 IDWIRGFYSSMTLFVSWGPRAAYINYIDFDLGVME----RITIDGMVK-ARVWGEKYFLS 507
Query: 57 N*NRLVRAKAAI 22
N +RLVRAK I
Sbjct: 508 NYDRLVRAKTLI 519
>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
Complex With (S)-Reticuline
Length = 519
Score = 203 bits (517), Expect(2) = 6e-62
Identities = 107/201 (53%), Positives = 143/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+C V + G I
Sbjct: 97 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGAI 156
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 157 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 215
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 216 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 273
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 274 TMLGFHFGLKTVAKSTFDLLF 294
Score = 63.2 bits (152), Expect(2) = 6e-62
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 7 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 66
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGGHS EGLSY+++
Sbjct: 67 TIRCIRKGSWTIRLRSGGHSYEGLSYTSD 95
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 415 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 473
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 474 NYERLIRAKTLI 485
>pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
Length = 498
Score = 207 bits (527), Expect(2) = 6e-62
Identities = 108/201 (53%), Positives = 144/201 (71%), Gaps = 1/201 (0%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PF++IDLM LN+V +DLES+TAW+E G+TLGE+Y+A+ SS+ LGF AG+C V + GHI
Sbjct: 94 PFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHI 153
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNVVD ILIDA+G LD ++MGEDVFWAIRGGG WGA+Y+W
Sbjct: 154 SGGGFGM-MSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAW 212
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVI-VKSETSILT 393
KI+L+ VPE VT RVT++ + EA LL+KWQ+VA +LE++FTL SV+ E +
Sbjct: 213 KIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTL--SVLGGADEKQVWL 270
Query: 392 VFQGLYHGPRSSAVASITELF 330
G + G ++ A ++ LF
Sbjct: 271 TMLGFHFGLKTVAKSTFDLLF 291
Score = 59.3 bits (142), Expect(2) = 6e-62
Identities = 33/89 (37%), Positives = 50/89 (56%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGVNYITPYT----QAYHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPA 1022
+ SC++ GV T ++ ++ L+ N ++ + KP AIILP +KE+L
Sbjct: 4 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 63
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
TI C RK +W RSGG S EGLSY+++
Sbjct: 64 TIRCIRKGSWTIRLRSGGASYEGLSYTSD 92
Score = 77.4 bits (189), Expect = 2e-11
Identities = 34/72 (47%), Positives = 49/72 (68%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
LDW+ ++E+M P++SK PR Y N ++LDLG +D G + + +E+ RSWGE YFL+
Sbjct: 412 LDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTV-VNNAIEISRSWGESYFLS 470
Query: 57 N*NRLVRAKAAI 22
N RL+RAK I
Sbjct: 471 NYERLIRAKTLI 482
>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
Length = 536
Score = 193 bits (490), Expect(2) = 2e-61
Identities = 97/200 (48%), Positives = 142/200 (71%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV+IDLM LN++ +D++S+TAW+E GAT+GE+Y+A+ S++ GF AG+C V + GHI
Sbjct: 123 PFVLIDLMNLNRISIDMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHI 182
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
MSRKYGLA+DNV D ILID++G LD + MGEDVFWA+RGGG WGA+Y+W
Sbjct: 183 SGGGFGM-MSRKYGLAADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGGVWGAIYAW 241
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASVIVKSETSILTV 390
KI+L+ VP+ VT RV ++ + EA+ L++KWQ+VA +L+D+FT+ + L V
Sbjct: 242 KIKLLPVPKKVTVFRVMKNVNIEEASFLIHKWQYVADELDDDFTVTILGGANGNGAWL-V 300
Query: 389 FQGLYHGPRSSAVASITELF 330
F GL+ G ++ A + + ++F
Sbjct: 301 FLGLHLGCKTVAKSIMDKMF 320
Score = 72.0 bits (175), Expect(2) = 2e-61
Identities = 39/89 (43%), Positives = 53/89 (59%), Gaps = 5/89 (5%)
Frame = -3
Query: 1186 NFFSCISSRGV-NYITPYTQA---YHHIFSLAADN-LRITSAYVKPVAIILPETKEQLPA 1022
+ SC++S GV NY TP + + Y +F L+ N L S KP I+LP KE+L
Sbjct: 33 DILSCLTSNGVHNYTTPSSDSNSDYLRLFHLSIQNPLFKKSTISKPSLIVLPGNKEELSN 92
Query: 1021 TISCCRKATWAFTYRSGGHSLEGLSYSAE 935
T+ CC + +W RSGGHS EGLSY+A+
Sbjct: 93 TVRCCTRGSWTIRLRSGGHSYEGLSYTAD 121
Score = 75.9 bits (185), Expect = 5e-11
Identities = 34/71 (47%), Positives = 49/71 (69%)
Frame = -1
Query: 234 DWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLAN 55
DW+ I++YM ++SK PR Y N ++LDLG +D N S++ +E+ R+WGEKYFL+N
Sbjct: 442 DWLRNIYDYMEEFVSKNPRVGYVNNIDLDLGGIDWSNKN-SSNNAIEIARNWGEKYFLSN 500
Query: 54 *NRLVRAKAAI 22
RL+RAK I
Sbjct: 501 YERLIRAKTLI 511
>ref|XP_003634619.1| PREDICTED: LOW QUALITY PROTEIN: reticuline oxidase-like [Vitis
vinifera]
Length = 712
Score = 210 bits (535), Expect(3) = 2e-61
Identities = 110/205 (53%), Positives = 141/205 (68%), Gaps = 5/205 (2%)
Frame = -2
Query: 929 PFVMIDLMKLNQVVVDLESKTAWIEGGATLGEVYHAVGTSSNHLGFPAGFCLAVRSSGHI 750
PFV+ID+M LNQV VD+ES+TAW+EGGATLGE Y+AV +SN GF AG C V GHI
Sbjct: 244 PFVIIDMMSLNQVSVDVESETAWVEGGATLGETYYAVAEASNVHGFSAGSCPTVGVGGHI 303
Query: 749 XXXXXXXFMSRKYGLASDNVVDGILIDADGRFLDCRSMGEDVFWAIRGGGARSWGAVYSW 570
+SRKYGLA+DNVVD +LIDADGR LD ++MGEDVFWAIRGGG WG VY+W
Sbjct: 304 AGGGFGL-LSRKYGLAADNVVDALLIDADGRVLDRKAMGEDVFWAIRGGGGGDWGIVYAW 362
Query: 569 KIRLVDVPETVTAPRVTRHGSKSEAAKLLNKWQWVALDLEDEFTLMASV-----IVKSET 405
KI+L+ VPETVT+ ++R +K A+L++KWQ++A LE F L V V ET
Sbjct: 363 KIKLLKVPETVTSCIMSRTRTKLHVAELVHKWQFIAPRLEPSFYLSVFVGAGLQGVDEET 422
Query: 404 SILTVFQGLYHGPRSSAVASITELF 330
+ F+G Y G R+ A++ + +F
Sbjct: 423 GVSASFKGFYLGSRNEAMSILNRVF 447
Score = 50.8 bits (120), Expect(3) = 2e-61
Identities = 24/61 (39%), Positives = 37/61 (60%), Gaps = 1/61 (1%)
Frame = -3
Query: 1126 YHHIFSLAADNLRITSAYV-KPVAIILPETKEQLPATISCCRKATWAFTYRSGGHSLEGL 950
Y+ + + + NLR KPVAI +P++ +QL ++ CCR+ + F R GGHS EG+
Sbjct: 176 YYRLLNFSLQNLRYAVPTAPKPVAIAIPQSLKQLVNSMRCCREGWYEFRVRCGGHSYEGI 235
Query: 949 S 947
S
Sbjct: 236 S 236
Score = 24.6 bits (52), Expect(3) = 2e-61
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = -1
Query: 348 INN*AFLELDVVASESKEMKWVESTAF 268
I N F EL V + +EM W+ES +
Sbjct: 442 ILNRVFPELGVEKEDCREMSWIESILY 468
Score = 79.3 bits (194), Expect = 4e-12
Identities = 37/72 (51%), Positives = 53/72 (73%)
Frame = -1
Query: 237 LDWVHGIHEYMTPYLSKGPRAAYENEVNLDLGVMD*GKHNISADELVELERSWGEKYFLA 58
+DW+ G +++M PY+S+GPRAAY N ++LDLG M+ +IS+++ VE R WGEKYFL
Sbjct: 570 IDWIRGFYKWMMPYVSQGPRAAYVNYMDLDLGQMN---SSISSNDPVEAARDWGEKYFLN 626
Query: 57 N*NRLVRAKAAI 22
N +RLV+ K I
Sbjct: 627 NYDRLVKVKTCI 638