BLASTX nr result
ID: Sinomenium22_contig00015630
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Sinomenium22_contig00015630 (327 letters) Database: ./nr 38,876,450 sequences; 13,856,398,315 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_002322733.2| ubiquitin-protein ligase [Populus trichocarp... 104 1e-20 ref|XP_006380888.1| hypothetical protein POPTR_0006s01330g [Popu... 100 3e-19 ref|XP_002308785.1| ubiquitin-protein ligase [Populus trichocarp... 100 3e-19 ref|XP_002322526.2| hypothetical protein POPTR_0016s01430g [Popu... 99 8e-19 ref|XP_007154018.1| hypothetical protein PHAVU_003G084200g [Phas... 97 2e-18 ref|XP_003549564.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 96 5e-18 ref|XP_006584091.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 96 7e-18 ref|XP_003528704.2| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 96 7e-18 ref|XP_003529662.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 96 7e-18 ref|XP_006365858.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 95 9e-18 ref|XP_004239830.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 95 9e-18 ref|XP_002272059.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 95 1e-17 emb|CBI33105.3| unnamed protein product [Vitis vinifera] 94 2e-17 ref|XP_007214612.1| hypothetical protein PRUPE_ppa001143mg [Prun... 94 3e-17 ref|XP_007026270.1| E3 ubiquitin-protein ligase UPL5 [Theobroma ... 92 1e-16 ref|XP_002532714.1| conserved hypothetical protein [Ricinus comm... 92 1e-16 ref|XP_006449870.1| hypothetical protein CICLE_v10014213mg [Citr... 90 3e-16 ref|XP_004295312.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 89 5e-16 gb|EXB41577.1| E3 ubiquitin-protein ligase UPL5 [Morus notabilis] 89 8e-16 ref|XP_006467329.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-... 89 8e-16 >ref|XP_002322733.2| ubiquitin-protein ligase [Populus trichocarpa] gi|550320938|gb|EEF04494.2| ubiquitin-protein ligase [Populus trichocarpa] Length = 592 Score = 104 bits (259), Expect = 1e-20 Identities = 55/108 (50%), Positives = 75/108 (69%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF+ LL +++CLGK+ D + + E G + Y+ ILKELNNI+K Sbjct: 374 YAKEIEQLHVIFIDLLNIMEKCLGKMQDSSNLKTNGESELNHTGWSQYLFILKELNNIAK 433 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 LY+GAE+K +R R+ L ALI RYAK++DDHQWLL+HKD+T FES Sbjct: 434 LYKGAEEKFWTVLRLRKASLCALIVRYAKQTDDHQWLLQHKDVTDFES 481 >ref|XP_006380888.1| hypothetical protein POPTR_0006s01330g [Populus trichocarpa] gi|550335213|gb|ERP58685.1| hypothetical protein POPTR_0006s01330g [Populus trichocarpa] Length = 860 Score = 100 bits (248), Expect = 3e-19 Identities = 53/108 (49%), Positives = 74/108 (68%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF+ LL+ +++CL K+ D + + E G + Y+ ILKELNNI+K Sbjct: 422 YAKEIEQLHVIFIDLLDIMEKCLEKMQDSSNLKTNGEGELNHTGWSQYLFILKELNNIAK 481 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 LY+GAE+K +R R+ L LI RYAKR+DDHQWLL+HKD+T F+S Sbjct: 482 LYKGAEEKFWTVLRLRKASLCVLIVRYAKRTDDHQWLLQHKDVTDFDS 529 >ref|XP_002308785.1| ubiquitin-protein ligase [Populus trichocarpa] gi|222854761|gb|EEE92308.1| ubiquitin-protein ligase [Populus trichocarpa] Length = 633 Score = 100 bits (248), Expect = 3e-19 Identities = 53/108 (49%), Positives = 74/108 (68%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF+ LL+ +++CL K+ D + + E G + Y+ ILKELNNI+K Sbjct: 422 YAKEIEQLHVIFIDLLDIMEKCLEKMQDSSNLKTNGEGELNHTGWSQYLFILKELNNIAK 481 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 LY+GAE+K +R R+ L LI RYAKR+DDHQWLL+HKD+T F+S Sbjct: 482 LYKGAEEKFWTVLRLRKASLCVLIVRYAKRTDDHQWLLQHKDVTDFDS 529 >ref|XP_002322526.2| hypothetical protein POPTR_0016s01430g [Populus trichocarpa] gi|550320566|gb|EEF04287.2| hypothetical protein POPTR_0016s01430g [Populus trichocarpa] Length = 853 Score = 98.6 bits (244), Expect = 8e-19 Identities = 53/108 (49%), Positives = 72/108 (66%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF LL +++CLGK+ D + E G + Y+ ILKELNNI+K Sbjct: 365 YAKEIEHLHVIFFDLLNIMEKCLGKMQDSSHLKMNGEGELNHTGWSQYLAILKELNNIAK 424 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 LY+GAE+K +R R+ L LI RYAKR++DHQWLL++KD+T FES Sbjct: 425 LYKGAEEKFWTVLRLRKASLCVLIVRYAKRTEDHQWLLRNKDVTDFES 472 >ref|XP_007154018.1| hypothetical protein PHAVU_003G084200g [Phaseolus vulgaris] gi|561027372|gb|ESW26012.1| hypothetical protein PHAVU_003G084200g [Phaseolus vulgaris] Length = 865 Score = 97.1 bits (240), Expect = 2e-18 Identities = 49/105 (46%), Positives = 72/105 (68%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ LH +++QLL KID+CL K+ L+ + + + +HY++ILKEL ISKLY+ Sbjct: 380 EIEYLHCLYIQLLNKIDQCLQKIDQSLVGQEMMEGDDLYTAWSHYLSILKELYQISKLYD 439 Query: 134 GAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 GAE KL +R + L LI RYAKR+D+HQW+L+H+ +T FES Sbjct: 440 GAEDKLWSVLRLHKSVLCLLIVRYAKRTDEHQWILEHRCVTNFES 484 >ref|XP_003549564.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Glycine max] Length = 867 Score = 95.9 bits (237), Expect = 5e-18 Identities = 47/105 (44%), Positives = 71/105 (67%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ LH +++QLL KID+CL K+ L + + + +HY++ILKEL ISKLY+ Sbjct: 382 EIEHLHGLYIQLLNKIDQCLQKMDQNLTGREMMEGDNLYPAWSHYLSILKELYQISKLYD 441 Query: 134 GAEKKL-CFTMRSRQLPLNALIRYAKRSDDHQWLLKHKDLTFFES 3 GAE+KL R R + ++RYAKR+D+HQW+L+H+ +T FES Sbjct: 442 GAEEKLWSILTRQRSVLCLLIVRYAKRTDEHQWILEHRCVTNFES 486 >ref|XP_006584091.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like isoform X2 [Glycine max] Length = 843 Score = 95.5 bits (236), Expect = 7e-18 Identities = 47/105 (44%), Positives = 72/105 (68%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ LH +++QLL KID+CL K+ L + + + +HY++ILKEL ISKLY+ Sbjct: 382 EIEYLHGLYVQLLNKIDQCLQKMDQSLAGQEMMEGDNLYPAWSHYLSILKELYQISKLYD 441 Query: 134 GAEKKLCFTM-RSRQLPLNALIRYAKRSDDHQWLLKHKDLTFFES 3 GAE+KL + R R + ++RYAKR+D+HQW+L+H+ +T FES Sbjct: 442 GAEEKLWGVLTRQRSVLCLLIVRYAKRTDEHQWILEHRYVTNFES 486 >ref|XP_003528704.2| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Glycine max] Length = 746 Score = 95.5 bits (236), Expect = 7e-18 Identities = 47/105 (44%), Positives = 72/105 (68%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ LH +++QLL KID+CL K+ L + + + +HY++ILKEL ISKLY+ Sbjct: 372 EIEYLHGLYVQLLNKIDQCLQKMDQSLAGQEMMEGDNLYPAWSHYLSILKELYQISKLYD 431 Query: 134 GAEKKLCFTM-RSRQLPLNALIRYAKRSDDHQWLLKHKDLTFFES 3 GAE+KL + R R + ++RYAKR+D+HQW+L+H+ +T FES Sbjct: 432 GAEEKLWGVLTRQRSVLCLLIVRYAKRTDEHQWILEHRYVTNFES 476 >ref|XP_003529662.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like isoform X1 [Glycine max] Length = 867 Score = 95.5 bits (236), Expect = 7e-18 Identities = 47/105 (44%), Positives = 72/105 (68%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ LH +++QLL KID+CL K+ L + + + +HY++ILKEL ISKLY+ Sbjct: 382 EIEYLHGLYVQLLNKIDQCLQKMDQSLAGQEMMEGDNLYPAWSHYLSILKELYQISKLYD 441 Query: 134 GAEKKLCFTM-RSRQLPLNALIRYAKRSDDHQWLLKHKDLTFFES 3 GAE+KL + R R + ++RYAKR+D+HQW+L+H+ +T FES Sbjct: 442 GAEEKLWGVLTRQRSVLCLLIVRYAKRTDEHQWILEHRYVTNFES 486 >ref|XP_006365858.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Solanum tuberosum] Length = 891 Score = 95.1 bits (235), Expect = 9e-18 Identities = 49/107 (45%), Positives = 72/107 (67%), Gaps = 1/107 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y ++K LHDI+ LLEK++ CL K+ + L + E + G + Y ILKE+N ISK Sbjct: 403 YRQQIKYLHDIYFDLLEKLELCLKKMEESLALKEKGEGEPLVSGWSQYFAILKEINAISK 462 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFE 6 LY+G+E + MR R++ L LI R+AKRS+DH+W+L+HK++T FE Sbjct: 463 LYKGSEDEFWNRMRQRKVSLCFLIVRFAKRSEDHRWILEHKEVTNFE 509 >ref|XP_004239830.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Solanum lycopersicum] Length = 891 Score = 95.1 bits (235), Expect = 9e-18 Identities = 49/107 (45%), Positives = 72/107 (67%), Gaps = 1/107 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y ++K LHDI+ LLEK++ CL K+ + L + E + G + Y ILKE+N ISK Sbjct: 403 YRQQIKCLHDIYFDLLEKLELCLKKMEESLALKEKGEGEPLVSGWSQYFAILKEINAISK 462 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFE 6 LY+G+E + MR R++ L LI R+AKRS+DH+W+L+HK++T FE Sbjct: 463 LYKGSEDEFWNRMRHRKVSLCFLIMRFAKRSEDHRWILEHKEVTNFE 509 >ref|XP_002272059.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Vitis vinifera] Length = 893 Score = 94.7 bits (234), Expect = 1e-17 Identities = 52/108 (48%), Positives = 69/108 (63%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF+ L+ K+D CL K+ L D Y+ +LKELN+ISK Sbjct: 408 YGEEIEFLHGIFIDLMTKMDGCLHKMEQCLAGEGGVDHHTVW---PQYLAVLKELNSISK 464 Query: 143 LYEGAEKKLCFTMRSRQLPLNAL-IRYAKRSDDHQWLLKHKDLTFFES 3 LY GAE++ MR R++ + +L IRYAKRSDDH WLL+HKD+T FES Sbjct: 465 LYHGAEEEFWTFMRRRKIAVCSLMIRYAKRSDDHSWLLEHKDVTDFES 512 >emb|CBI33105.3| unnamed protein product [Vitis vinifera] Length = 831 Score = 94.0 bits (232), Expect = 2e-17 Identities = 51/105 (48%), Positives = 68/105 (64%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ LH IF+ L+ K+D CL K+ L D Y+ +LKELN+ISKLY Sbjct: 349 EIEFLHGIFIDLMTKMDGCLHKMEQCLAGEGGVDHHTVW---PQYLAVLKELNSISKLYH 405 Query: 134 GAEKKLCFTMRSRQLPLNAL-IRYAKRSDDHQWLLKHKDLTFFES 3 GAE++ MR R++ + +L IRYAKRSDDH WLL+HKD+T FES Sbjct: 406 GAEEEFWTFMRRRKIAVCSLMIRYAKRSDDHSWLLEHKDVTDFES 450 >ref|XP_007214612.1| hypothetical protein PRUPE_ppa001143mg [Prunus persica] gi|462410477|gb|EMJ15811.1| hypothetical protein PRUPE_ppa001143mg [Prunus persica] Length = 897 Score = 93.6 bits (231), Expect = 3e-17 Identities = 51/108 (47%), Positives = 71/108 (65%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ L DI LL K+DECLGK+G+ L + + G + Y++ILKEL+ I Sbjct: 408 YGEEIELLRDIQADLLLKMDECLGKMGEFLAGKGKGEGDIVHSGWSQYLSILKELSGICI 467 Query: 143 LYEGAEKKLCFTMRSRQLPLNAL-IRYAKRSDDHQWLLKHKDLTFFES 3 LY+G E++L +R R+ L AL ++ AKRSDDHQWL++HKDL FES Sbjct: 468 LYQGGEEQLKNILRLRRASLCALVVKCAKRSDDHQWLIQHKDLLDFES 515 >ref|XP_007026270.1| E3 ubiquitin-protein ligase UPL5 [Theobroma cacao] gi|508781636|gb|EOY28892.1| E3 ubiquitin-protein ligase UPL5 [Theobroma cacao] Length = 899 Score = 91.7 bits (226), Expect = 1e-16 Identities = 47/108 (43%), Positives = 72/108 (66%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF LL K+++CL ++ + +D G + Y+ ILKELN ISK Sbjct: 411 YGEEIEFLHAIFNDLLAKMEKCLVRMEENFAVRGSRDGGVVHSGSSQYLAILKELNGISK 470 Query: 143 LYEGAEKKLCFTMRSRQLPL-NALIRYAKRSDDHQWLLKHKDLTFFES 3 LYEGAE++ +R+R+ L + +I +A+R+DD++WLL+HKD+T FES Sbjct: 471 LYEGAEEQFWMVLRNRKSSLCSLIISFARRTDDNRWLLEHKDVTDFES 518 >ref|XP_002532714.1| conserved hypothetical protein [Ricinus communis] gi|223527541|gb|EEF29663.1| conserved hypothetical protein [Ricinus communis] Length = 876 Score = 91.7 bits (226), Expect = 1e-16 Identities = 48/108 (44%), Positives = 72/108 (66%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E+++L+DIF+ L+ K+D CL K+ D L + E+ + Y+ ILKELNNI+K Sbjct: 420 YAEEIENLYDIFVDLMMKMDWCLTKMEDFLPMKPNGEGESACTRWSQYLAILKELNNIAK 479 Query: 143 LYEGAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 Y+ AE++ ++ + L LI +YAKR+DD+QWLL+HKD+T FES Sbjct: 480 HYKKAEEEFWSVLKRTKASLCVLIVKYAKRNDDNQWLLQHKDVTDFES 527 >ref|XP_006449870.1| hypothetical protein CICLE_v10014213mg [Citrus clementina] gi|557552481|gb|ESR63110.1| hypothetical protein CICLE_v10014213mg [Citrus clementina] Length = 889 Score = 90.1 bits (222), Expect = 3e-16 Identities = 46/105 (43%), Positives = 76/105 (72%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 ++ S++ +F LL K+D+CL +V + ++A + + E G + Y+TIL+EL+ SKL++ Sbjct: 404 QLDSIYGMFCDLLIKMDKCLVRVQERVIARANGEGEINYSGWSQYLTILRELHATSKLFQ 463 Query: 134 GAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 GAE++ +R+R+ PL LI RYA+RSDD++WLL+HKD+T F+S Sbjct: 464 GAEEEFWKLLRNRKSPLGDLILRYARRSDDNRWLLEHKDVTTFDS 508 >ref|XP_004295312.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Fragaria vesca subsp. vesca] Length = 907 Score = 89.4 bits (220), Expect = 5e-16 Identities = 46/105 (43%), Positives = 68/105 (64%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 E++ L +I LL ++ ECL KV + L + D + + Y+ +LKEL+ I KLY+ Sbjct: 422 EIELLRNILTDLLNRMAECLTKVVEYLAGKAKGDSDIVHTRWSQYLAVLKELHGICKLYQ 481 Query: 134 GAEKKLCFTMRSRQLPLNAL-IRYAKRSDDHQWLLKHKDLTFFES 3 G E++ T+R R+ + AL ++YAKRSDDHQWLL+HKD+ FES Sbjct: 482 GGEEQFWTTLRLRKTAICALVVKYAKRSDDHQWLLEHKDVLDFES 526 >gb|EXB41577.1| E3 ubiquitin-protein ligase UPL5 [Morus notabilis] Length = 1023 Score = 88.6 bits (218), Expect = 8e-16 Identities = 48/108 (44%), Positives = 69/108 (63%), Gaps = 1/108 (0%) Frame = -2 Query: 323 YVVEVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISK 144 Y E++ LH IF QLL ++D+CL K+ D L D + + Y+ ILKELN+ISK Sbjct: 430 YGEEIEFLHRIFRQLLCRMDQCLLKMEDHLAGKGKGDGDIAHTRWSQYLAILKELNSISK 489 Query: 143 LYEGAEKKLCFTMRSRQLPLNAL-IRYAKRSDDHQWLLKHKDLTFFES 3 LYE AE++ +R R+ AL + YA+R+DD+QW++ HKD+ FES Sbjct: 490 LYEDAEERFWAVLRLRRSSFCALVVNYARRTDDNQWIVNHKDVLDFES 537 >ref|XP_006467329.1| PREDICTED: E3 ubiquitin-protein ligase UPL5-like [Citrus sinensis] Length = 889 Score = 88.6 bits (218), Expect = 8e-16 Identities = 46/105 (43%), Positives = 75/105 (71%), Gaps = 1/105 (0%) Frame = -2 Query: 314 EVKSLHDIFLQLLEKIDECLGKVGDILMANSVKDLEACLIGCTHYITILKELNNISKLYE 135 ++ S++ +F LL K+D+CL +V + ++A + + E G + Y+TIL+EL+ SKL++ Sbjct: 404 QLDSIYGMFCDLLIKMDKCLVRVQERVIARANGEGEINYSGWSQYLTILRELHATSKLFQ 463 Query: 134 GAEKKLCFTMRSRQLPLNALI-RYAKRSDDHQWLLKHKDLTFFES 3 GAE++ +R+R PL LI RYA+RSDD++WLL+HKD+T F+S Sbjct: 464 GAEEEFWKLLRNRISPLGDLILRYARRSDDNRWLLEHKDVTTFDS 508