BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sinomenium22_contig00011009
         (422 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    71   1e-11
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...    70   1e-11
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...    70   1e-11
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...    64   2e-09
gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    61   9e-09
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    63   1e-08
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]         65   1e-08
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...    64   3e-08
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...    64   3e-08
gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum] gi|388...    63   5e-08
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...    62   8e-08
gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum f...    62   8e-08
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt...    61   1e-07
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth...    61   1e-07
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap...    60   2e-07
ref|XP_006858228.1| hypothetical protein AMTR_s00062p00190100 [A...    60   3e-07
gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Pap...    60   4e-07
sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...    59   7e-07

>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score = 71.2 bits (173), Expect(2) = 1e-11
 Identities = 38/66 (57%), Positives = 48/66 (72%), Gaps = 1/66 (1%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEI-DGLKRYSVTPAAKLLLRNT 404
           +P+ L DLASKLPVSN + D LYRILRYLV   +L V +  DG K+Y++ P A LL RN 
Sbjct: 48  QPMALADLASKLPVSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNA 107

Query: 405 EKSMVP 422
           ++SMVP
Sbjct: 108 KRSMVP 113



 Score = 23.9 bits (50), Expect(2) = 1e-11
 Identities = 7/13 (53%), Positives = 12/13 (92%)
 Frame = +2

Query: 113 VDVRDQAHLWKLI 151
           +D++ QAH+WK+I
Sbjct: 10  LDIKAQAHVWKII 22


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
           4'-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:3'-hydroxy-N-
           methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
           gi|9711916|dbj|BAB08005.1|
           S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
           4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score = 69.7 bits (169), Expect(2) = 1e-11
 Identities = 37/66 (56%), Positives = 48/66 (72%), Gaps = 1/66 (1%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEI-DGLKRYSVTPAAKLLLRNT 404
           +P+ L DLASKLPVS+ + D LYRILRYLV   +L V +  DG K+Y++ P A LL RN 
Sbjct: 48  QPMALADLASKLPVSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNA 107

Query: 405 EKSMVP 422
           ++SMVP
Sbjct: 108 KRSMVP 113



 Score = 25.4 bits (54), Expect(2) = 1e-11
 Identities = 8/14 (57%), Positives = 13/14 (92%)
 Frame = +2

Query: 110 VVDVRDQAHLWKLI 151
           V+D++ QAH+WK+I
Sbjct: 9   VLDIKAQAHVWKII 22


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
           [Thalictrum flavum subsp. glaucum]
          Length = 348

 Score = 70.1 bits (170), Expect(2) = 1e-11
 Identities = 37/65 (56%), Positives = 48/65 (73%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNTE 407
           +PI L DLASKLP+SN + D L+RILRYLV   LL+ A  D   +Y++ P AKL+LRN +
Sbjct: 49  QPIPLADLASKLPISNVNLDNLFRILRYLVKMELLSYAADD---KYALEPIAKLVLRNEK 105

Query: 408 KSMVP 422
           +SMVP
Sbjct: 106 RSMVP 110



 Score = 25.0 bits (53), Expect(2) = 1e-11
 Identities = 8/13 (61%), Positives = 12/13 (92%)
 Frame = +2

Query: 113 VDVRDQAHLWKLI 151
           VD++ QAH+WK+I
Sbjct: 11  VDIKAQAHVWKII 23


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
           californica]
          Length = 353

 Score = 63.5 bits (153), Expect(2) = 2e-09
 Identities = 34/68 (50%), Positives = 48/68 (70%), Gaps = 5/68 (7%)
 Frame = +3

Query: 234 ITLFDLASKLPVSNTSRDRLYRILRYLVHARLL--TVAEIDG---LKRYSVTPAAKLLLR 398
           IT+ +LASKLP+SN + D LYR+LRYLVH  +L  T + I+G    K YS+ P   LL++
Sbjct: 49  ITVSELASKLPISNVNSDNLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVK 108

Query: 399 NTEKSMVP 422
           + E++MVP
Sbjct: 109 DAERNMVP 116



 Score = 24.3 bits (51), Expect(2) = 2e-09
 Identities = 8/13 (61%), Positives = 11/13 (84%)
 Frame = +2

Query: 113 VDVRDQAHLWKLI 151
           VD++ QAHLW +I
Sbjct: 9   VDIKAQAHLWNII 21


>gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase 1 [Papaver somniferum]
          Length = 354

 Score = 60.8 bits (146), Expect(2) = 9e-09
 Identities = 33/67 (49%), Positives = 45/67 (67%), Gaps = 4/67 (5%)
 Frame = +3

Query: 234 ITLFDLASKLPVSNTSRDRLYRILRYLVHARLL----TVAEIDGLKRYSVTPAAKLLLRN 401
           ITL +L SKLP+SN + D LYR+LRYLVH  +L      A +D  + YS+ P   LLL++
Sbjct: 52  ITLSELVSKLPLSNVNSDNLYRLLRYLVHLNILGQQTCAAGVD--RVYSLKPVGTLLLKD 109

Query: 402 TEKSMVP 422
           +E+SM P
Sbjct: 110 SERSMAP 116



 Score = 24.3 bits (51), Expect(2) = 9e-09
 Identities = 8/13 (61%), Positives = 11/13 (84%)
 Frame = +2

Query: 113 VDVRDQAHLWKLI 151
           VD++DQA LW +I
Sbjct: 12  VDIKDQAQLWNII 24


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase 2 [Papaver somniferum]
           gi|571330882|gb|AHF27397.1|
           S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'- O-methyltransferase 2 [synthetic construct]
          Length = 357

 Score = 62.8 bits (151), Expect(2) = 1e-08
 Identities = 33/65 (50%), Positives = 44/65 (67%), Gaps = 2/65 (3%)
 Frame = +3

Query: 234 ITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDG--LKRYSVTPAAKLLLRNTE 407
           ITL  LA+KLP++N S D LYR++RYLVH  ++     +G   K YS+ P   LLLR+ E
Sbjct: 55  ITLAQLAAKLPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAE 114

Query: 408 KSMVP 422
           +SMVP
Sbjct: 115 RSMVP 119



 Score = 21.9 bits (45), Expect(2) = 1e-08
 Identities = 7/13 (53%), Positives = 10/13 (76%)
 Frame = +2

Query: 113 VDVRDQAHLWKLI 151
           V ++DQA LW +I
Sbjct: 15  VSIKDQAQLWNII 27


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score = 64.7 bits (156), Expect = 1e-08
 Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLK--RYSVTPAAKLLLRN 401
           KP+TL +LASKLPV     DRLYR++RYLVH +L    E +  +  +YS+ P AK L++ 
Sbjct: 47  KPMTLSELASKLPVQPVDLDRLYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKG 106

Query: 402 TEKSMV 419
            EKSMV
Sbjct: 107 WEKSMV 112


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase; Short=6-OMT
           gi|9711914|dbj|BAB08004.1|
           S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score = 63.5 bits (153), Expect = 3e-08
 Identities = 30/62 (48%), Positives = 42/62 (67%)
 Frame = +3

Query: 234 ITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNTEKS 413
           +TL +L+S+LP    + D LYR++RYLVH +L T A IDG  RY + P AK L++  +K 
Sbjct: 46  MTLSELSSRLPSQPVNEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKC 105

Query: 414 MV 419
           MV
Sbjct: 106 MV 107


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum
           flavum]
          Length = 346

 Score = 63.5 bits (153), Expect = 3e-08
 Identities = 28/63 (44%), Positives = 43/63 (68%)
 Frame = +3

Query: 231 PITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNTEK 410
           P+TL +L+S+LP    ++D LYR++RYLVH +L T + IDG  RY + P AK L++  + 
Sbjct: 41  PMTLSELSSRLPSQPVNQDALYRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDN 100

Query: 411 SMV 419
            M+
Sbjct: 101 CMI 103


>gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum]
           gi|388571230|gb|AFK73711.1| O-methyltransferase [Papaver
           somniferum]
          Length = 339

 Score = 62.8 bits (151), Expect = 5e-08
 Identities = 33/65 (50%), Positives = 44/65 (67%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNTE 407
           KP++L +LASKLP    + DRLYRIL +LVH +L    +    ++YS+ P AK LL+  E
Sbjct: 45  KPMSLSELASKLPAQPVNEDRLYRILHFLVHMKLFN--KDATTQKYSLAPPAKYLLKGWE 102

Query: 408 KSMVP 422
           KSMVP
Sbjct: 103 KSMVP 107


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
           glaucum]
          Length = 350

 Score = 62.0 bits (149), Expect = 8e-08
 Identities = 29/63 (46%), Positives = 42/63 (66%)
 Frame = +3

Query: 231 PITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNTEK 410
           P+TL +L+  LP    ++D LYR+LRYLVH +L T + IDG  RY + P AK L++  +K
Sbjct: 46  PMTLSELSLHLPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDK 105

Query: 411 SMV 419
            M+
Sbjct: 106 CML 108


>gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum flavum]
          Length = 225

 Score = 62.0 bits (149), Expect = 8e-08
 Identities = 29/63 (46%), Positives = 42/63 (66%)
 Frame = +3

Query: 231 PITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNTEK 410
           P+TL +L+  LP    ++D LYR+LRYLVH +L T + IDG  RY + P AK L++  +K
Sbjct: 46  PMTLSELSLHLPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDK 105

Query: 411 SMV 419
            M+
Sbjct: 106 CML 108


>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
           [Papaver somniferum]
          Length = 346

 Score = 61.2 bits (147), Expect = 1e-07
 Identities = 34/65 (52%), Positives = 45/65 (69%), Gaps = 1/65 (1%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSN-TSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNT 404
           KP++L +LASKLPV+   + DRL+RI+RYLVH  L  +      ++YS+ P AK LLR  
Sbjct: 45  KPMSLSELASKLPVAQPVNEDRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGW 102

Query: 405 EKSMV 419
           EKSMV
Sbjct: 103 EKSMV 107


>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=6OMT
           gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
           6-O-methyltransferase [Papaver somniferum]
           gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
           6-O-methyltransferase [synthetic construct]
          Length = 346

 Score = 61.2 bits (147), Expect = 1e-07
 Identities = 34/65 (52%), Positives = 45/65 (69%), Gaps = 1/65 (1%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSN-TSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNT 404
           KP++L +LASKLPV+   + DRL+RI+RYLVH  L  +      ++YS+ P AK LLR  
Sbjct: 45  KPMSLSELASKLPVAQPVNEDRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGW 102

Query: 405 EKSMV 419
           EKSMV
Sbjct: 103 EKSMV 107


>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 346

 Score = 60.5 bits (145), Expect = 2e-07
 Identities = 34/65 (52%), Positives = 44/65 (67%), Gaps = 1/65 (1%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSN-TSRDRLYRILRYLVHARLLTVAEIDGLKRYSVTPAAKLLLRNT 404
           KP++L +LASKLPV    + DRL+RI+RYLVH  L  +      ++YS+ P AK LLR  
Sbjct: 45  KPMSLSELASKLPVPQPVNEDRLFRIMRYLVHMELFKIDAT--TQKYSLAPPAKYLLRGW 102

Query: 405 EKSMV 419
           EKSMV
Sbjct: 103 EKSMV 107


>ref|XP_006858228.1| hypothetical protein AMTR_s00062p00190100 [Amborella trichopoda]
           gi|548862331|gb|ERN19695.1| hypothetical protein
           AMTR_s00062p00190100 [Amborella trichopoda]
          Length = 353

 Score = 60.1 bits (144), Expect = 3e-07
 Identities = 33/67 (49%), Positives = 47/67 (70%), Gaps = 4/67 (5%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVAEIDGL----KRYSVTPAAKLLL 395
           +PITLF LAS LP+   + DRL+R++R+LVH  L   A+  GL    + Y +TP+++LLL
Sbjct: 52  RPITLFHLASLLPIQPVNPDRLHRLMRFLVHTHLF--AKTTGLNGDEETYWLTPSSQLLL 109

Query: 396 RNTEKSM 416
           R+ EKSM
Sbjct: 110 RHAEKSM 116


>gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 357

 Score = 59.7 bits (143), Expect = 4e-07
 Identities = 33/75 (44%), Positives = 47/75 (62%), Gaps = 10/75 (13%)
 Frame = +3

Query: 228 KPITLFDLASKLPVSN-TSRDRLYRILRYLVHARLLTVAEIDGLK---------RYSVTP 377
           KP++L +LASKLP+    + DRLYR++R+LVH +L     I  L          +YS+ P
Sbjct: 45  KPMSLSELASKLPIDQPVNIDRLYRVMRFLVHMKLFNKENISILSGETAQVVEDKYSLAP 104

Query: 378 AAKLLLRNTEKSMVP 422
            AK L+R +E+SMVP
Sbjct: 105 PAKYLIRGSEQSMVP 119


>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT;
           AltName: Full=Tetrahydrocolumbamine
           2-O-methyltransferase gi|24210466|dbj|BAC22084.1|
           columbamine O-methyltransferase [Coptis japonica]
          Length = 351

 Score = 58.9 bits (141), Expect = 7e-07
 Identities = 28/66 (42%), Positives = 44/66 (66%), Gaps = 2/66 (3%)
 Frame = +3

Query: 231 PITLFDLASKLPVSNTSRDRLYRILRYLVHARLLTVA--EIDGLKRYSVTPAAKLLLRNT 404
           P+TL  L + LP+ +TS DR +  +RYLVH +L T++  +I    +Y +TPA+KLL+   
Sbjct: 49  PVTLSQLVTHLPLKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGH 108

Query: 405 EKSMVP 422
           +KS+ P
Sbjct: 109 QKSLAP 114