BLASTX nr result
ID: Sinomenium21_contig00040639
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium21_contig00040639
(360 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004294016.1| PREDICTED: uncharacterized protein LOC101293... 134 2e-29
ref|XP_007025610.1| F3H9.11 protein isoform 5 [Theobroma cacao] ... 132 7e-29
ref|XP_007025609.1| F3H9.11 protein isoform 4 [Theobroma cacao] ... 132 7e-29
ref|XP_007025608.1| F3H9.11 protein isoform 3 [Theobroma cacao] ... 132 7e-29
ref|XP_007025607.1| F3H9.11 protein isoform 2 [Theobroma cacao] ... 132 7e-29
ref|XP_007025606.1| F3H9.11 protein isoform 1 [Theobroma cacao] ... 132 7e-29
ref|XP_004505059.1| PREDICTED: uncharacterized protein LOC101507... 131 1e-28
ref|XP_007211416.1| hypothetical protein PRUPE_ppa003865mg [Prun... 131 1e-28
ref|XP_006377366.1| hypothetical protein POPTR_0011s05280g [Popu... 130 2e-28
ref|XP_007159328.1| hypothetical protein PHAVU_002G228900g [Phas... 130 2e-28
gb|EYU43390.1| hypothetical protein MIMGU_mgv1a021937mg [Mimulus... 128 7e-28
ref|XP_006594318.1| PREDICTED: uncharacterized protein LOC100779... 128 7e-28
ref|XP_003542746.2| PREDICTED: uncharacterized protein LOC100779... 128 7e-28
ref|XP_004163444.1| PREDICTED: uncharacterized LOC101209711 [Cuc... 128 7e-28
ref|XP_004146603.1| PREDICTED: LOW QUALITY PROTEIN: uncharacteri... 127 1e-27
ref|XP_006415681.1| hypothetical protein EUTSA_v10007204mg [Eutr... 127 2e-27
ref|XP_002509470.1| conserved hypothetical protein [Ricinus comm... 127 2e-27
ref|XP_006583820.1| PREDICTED: uncharacterized protein LOC100783... 126 3e-27
ref|XP_006583819.1| PREDICTED: uncharacterized protein LOC100783... 126 3e-27
ref|XP_006343239.1| PREDICTED: uncharacterized protein LOC102601... 126 4e-27
>ref|XP_004294016.1| PREDICTED: uncharacterized protein LOC101293376 [Fragaria vesca
subsp. vesca]
Length = 588
Score = 134 bits (336), Expect = 2e-29
Identities = 67/112 (59%), Positives = 77/112 (68%), Gaps = 6/112 (5%)
Frame = +1
Query: 43 PSSSHSMSVSGKNKSRRSPHP-----VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVC 207
P S N S P P +G TLPPGHPC F+L PPP DKKRTGPRPCPVC
Sbjct: 115 PESLAQTEKQSYNVSLALPPPPPAFFLGYTLPPGHPCNIFTLPPPPADKKRTGPRPCPVC 174
Query: 208 YLPVEKAMVSMPAVTSLSPVLRNLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
YLPVE+A+ MP V S SP+L+NLTY+ E+N R TEFGGS FGG+P+LKQR
Sbjct: 175 YLPVEEAIALMPKVPSYSPILKNLTYIYEENLSRETEFGGSDFGGYPTLKQR 226
>ref|XP_007025610.1| F3H9.11 protein isoform 5 [Theobroma cacao]
gi|508780976|gb|EOY28232.1| F3H9.11 protein isoform 5
[Theobroma cacao]
Length = 616
Score = 132 bits (331), Expect = 7e-29
Identities = 61/86 (70%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC FSL PPP DKKRTGPRPCPVCYLPVE A+ MP V S SPV++NLTY
Sbjct: 142 LGYTLPPGHPCATFSLPPPPADKKRTGPRPCPVCYLPVEDAIALMPKVPSFSPVVKNLTY 201
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+ E+N + TEFGGS FGG+P+LKQR
Sbjct: 202 IYEENLNKETEFGGSDFGGYPTLKQR 227
>ref|XP_007025609.1| F3H9.11 protein isoform 4 [Theobroma cacao]
gi|508780975|gb|EOY28231.1| F3H9.11 protein isoform 4
[Theobroma cacao]
Length = 516
Score = 132 bits (331), Expect = 7e-29
Identities = 61/86 (70%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC FSL PPP DKKRTGPRPCPVCYLPVE A+ MP V S SPV++NLTY
Sbjct: 72 LGYTLPPGHPCATFSLPPPPADKKRTGPRPCPVCYLPVEDAIALMPKVPSFSPVVKNLTY 131
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+ E+N + TEFGGS FGG+P+LKQR
Sbjct: 132 IYEENLNKETEFGGSDFGGYPTLKQR 157
>ref|XP_007025608.1| F3H9.11 protein isoform 3 [Theobroma cacao]
gi|508780974|gb|EOY28230.1| F3H9.11 protein isoform 3
[Theobroma cacao]
Length = 518
Score = 132 bits (331), Expect = 7e-29
Identities = 61/86 (70%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC FSL PPP DKKRTGPRPCPVCYLPVE A+ MP V S SPV++NLTY
Sbjct: 142 LGYTLPPGHPCATFSLPPPPADKKRTGPRPCPVCYLPVEDAIALMPKVPSFSPVVKNLTY 201
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+ E+N + TEFGGS FGG+P+LKQR
Sbjct: 202 IYEENLNKETEFGGSDFGGYPTLKQR 227
>ref|XP_007025607.1| F3H9.11 protein isoform 2 [Theobroma cacao]
gi|508780973|gb|EOY28229.1| F3H9.11 protein isoform 2
[Theobroma cacao]
Length = 511
Score = 132 bits (331), Expect = 7e-29
Identities = 61/86 (70%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC FSL PPP DKKRTGPRPCPVCYLPVE A+ MP V S SPV++NLTY
Sbjct: 67 LGYTLPPGHPCATFSLPPPPADKKRTGPRPCPVCYLPVEDAIALMPKVPSFSPVVKNLTY 126
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+ E+N + TEFGGS FGG+P+LKQR
Sbjct: 127 IYEENLNKETEFGGSDFGGYPTLKQR 152
>ref|XP_007025606.1| F3H9.11 protein isoform 1 [Theobroma cacao]
gi|508780972|gb|EOY28228.1| F3H9.11 protein isoform 1
[Theobroma cacao]
Length = 586
Score = 132 bits (331), Expect = 7e-29
Identities = 61/86 (70%), Positives = 70/86 (81%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC FSL PPP DKKRTGPRPCPVCYLPVE A+ MP V S SPV++NLTY
Sbjct: 142 LGYTLPPGHPCATFSLPPPPADKKRTGPRPCPVCYLPVEDAIALMPKVPSFSPVVKNLTY 201
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+ E+N + TEFGGS FGG+P+LKQR
Sbjct: 202 IYEENLNKETEFGGSDFGGYPTLKQR 227
>ref|XP_004505059.1| PREDICTED: uncharacterized protein LOC101507527 [Cicer arietinum]
Length = 570
Score = 131 bits (329), Expect = 1e-28
Identities = 66/111 (59%), Positives = 78/111 (70%), Gaps = 4/111 (3%)
Frame = +1
Query: 40 IPSSSHSMSVSGKNKSRRSPHP---VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCY 210
+ S S +S N + P P +G LP GHPC F+L PPP DKKRTGPRPCPVCY
Sbjct: 104 VSMSDSSSEISTANFALLPPPPSYFLGYHLPTGHPCNSFTLPPPPADKKRTGPRPCPVCY 163
Query: 211 LPVEKAMVSMPAVTSLSPVLRNLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
LPVE+A+ MP + SLSPVL+NLTYV E+N R EFGGS FGG+P+LKQR
Sbjct: 164 LPVEEAIARMPTLPSLSPVLQNLTYVYEENLSRGDEFGGSEFGGYPTLKQR 214
>ref|XP_007211416.1| hypothetical protein PRUPE_ppa003865mg [Prunus persica]
gi|462407281|gb|EMJ12615.1| hypothetical protein
PRUPE_ppa003865mg [Prunus persica]
Length = 543
Score = 131 bits (329), Expect = 1e-28
Identities = 61/86 (70%), Positives = 71/86 (82%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC F+L PPP DKKRTGPRPCPVCYLPVE+A+ MP V S SPVL+NLTY
Sbjct: 91 LGYTLPPGHPCNLFTLPPPPADKKRTGPRPCPVCYLPVEEAIALMPKVPSDSPVLKNLTY 150
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+ E+N R TEFGGS FGG+P+L+QR
Sbjct: 151 IYEENLSRETEFGGSDFGGYPTLQQR 176
>ref|XP_006377366.1| hypothetical protein POPTR_0011s05280g [Populus trichocarpa]
gi|550327655|gb|ERP55163.1| hypothetical protein
POPTR_0011s05280g [Populus trichocarpa]
Length = 591
Score = 130 bits (327), Expect = 2e-28
Identities = 60/86 (69%), Positives = 69/86 (80%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G TLPPGHPC F+L PPP DKKRTGPRPCPVCYLPVE+A+ MP V S SPV++NLTY
Sbjct: 138 LGYTLPPGHPCNRFTLPPPPADKKRTGPRPCPVCYLPVEEAIALMPKVPSFSPVIKNLTY 197
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
+QE R EFGGS FGG+P+LKQR
Sbjct: 198 IQEDPLSREGEFGGSDFGGYPTLKQR 223
>ref|XP_007159328.1| hypothetical protein PHAVU_002G228900g [Phaseolus vulgaris]
gi|561032743|gb|ESW31322.1| hypothetical protein
PHAVU_002G228900g [Phaseolus vulgaris]
Length = 566
Score = 130 bits (326), Expect = 2e-28
Identities = 65/106 (61%), Positives = 78/106 (73%), Gaps = 1/106 (0%)
Frame = +1
Query: 46 SSSHSMSVSGKNKSRRSPHPVGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEK 225
S++ +M ++ K S + +G LPPGHPC F+L PPP DKKRTGPRPCPVCYLPVE+
Sbjct: 104 STTKAMGLTTKLSSPPPGYFLGYYLPPGHPCNSFTLPPPPADKKRTGPRPCPVCYLPVEE 163
Query: 226 AMVSMPAVTSLSPVLRNLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
A+ MP S SPVLRNLTYV E+N R EFGGS FGG+P+LKQR
Sbjct: 164 AIGLMPKFPSPSPVLRNLTYVYEENLSRDGEFGGSEFGGYPTLKQR 209
>gb|EYU43390.1| hypothetical protein MIMGU_mgv1a021937mg [Mimulus guttatus]
Length = 577
Score = 128 bits (322), Expect = 7e-28
Identities = 67/127 (52%), Positives = 82/127 (64%), Gaps = 16/127 (12%)
Frame = +1
Query: 28 RKVYIPSSSHSMSVSGKNKSRRS-------------PHPV---GSTLPPGHPCQHFSLTP 159
R V P S +SVS + SR + P PV G LPPG+PC+HF+L P
Sbjct: 96 RTVPGPLSEEKVSVSETSSSRITAIERSVNVTLPPPPPPVYFTGYKLPPGNPCEHFTLPP 155
Query: 160 PPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTYVQEKNPRVTEFGGSWFGG 339
PP DKKRTGPRPCPVCYLP+E A+ MP S SPVL++LTY+ ++N +EFGGS FGG
Sbjct: 156 PPADKKRTGPRPCPVCYLPLEDAVTLMPNAPSFSPVLKDLTYIHDENLTKSEFGGSEFGG 215
Query: 340 HPSLKQR 360
+PSL QR
Sbjct: 216 YPSLGQR 222
>ref|XP_006594318.1| PREDICTED: uncharacterized protein LOC100779951 isoform X2 [Glycine
max]
Length = 549
Score = 128 bits (322), Expect = 7e-28
Identities = 66/113 (58%), Positives = 80/113 (70%), Gaps = 8/113 (7%)
Frame = +1
Query: 46 SSSHSMSVSGKNKSRRSPHP-------VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPV 204
S+S + + + ++RS P +G LPPGHPC F+L PPP DKKRTGPRPCPV
Sbjct: 82 STSENSTTNAMGLTKRSVLPPPPAGYFLGYRLPPGHPCNSFTLPPPPADKKRTGPRPCPV 141
Query: 205 CYLPVEKAMVSMPAVTSLSPVLRNLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
CYLPVE+A+ MPA+ S SPVL NLTYV E+N R EFGGS FGG+P+LKQR
Sbjct: 142 CYLPVEEAIGLMPALPSPSPVLGNLTYVYEENLSRDGEFGGSDFGGYPTLKQR 194
>ref|XP_003542746.2| PREDICTED: uncharacterized protein LOC100779951 isoform X1 [Glycine
max]
Length = 567
Score = 128 bits (322), Expect = 7e-28
Identities = 66/113 (58%), Positives = 80/113 (70%), Gaps = 8/113 (7%)
Frame = +1
Query: 46 SSSHSMSVSGKNKSRRSPHP-------VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPV 204
S+S + + + ++RS P +G LPPGHPC F+L PPP DKKRTGPRPCPV
Sbjct: 100 STSENSTTNAMGLTKRSVLPPPPAGYFLGYRLPPGHPCNSFTLPPPPADKKRTGPRPCPV 159
Query: 205 CYLPVEKAMVSMPAVTSLSPVLRNLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
CYLPVE+A+ MPA+ S SPVL NLTYV E+N R EFGGS FGG+P+LKQR
Sbjct: 160 CYLPVEEAIGLMPALPSPSPVLGNLTYVYEENLSRDGEFGGSDFGGYPTLKQR 212
>ref|XP_004163444.1| PREDICTED: uncharacterized LOC101209711 [Cucumis sativus]
Length = 583
Score = 128 bits (322), Expect = 7e-28
Identities = 59/89 (66%), Positives = 70/89 (78%), Gaps = 1/89 (1%)
Frame = +1
Query: 97 PHPVGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRN 276
P P G TLPPGHPC +F+L PPP DKKRTGPRPCPVCYLPVE+A+ MP +S SPVL+
Sbjct: 137 PPPPGYTLPPGHPCNNFALPPPPADKKRTGPRPCPVCYLPVEEAVALMPNASSDSPVLKK 196
Query: 277 LTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
L Y+ E+N R TEFGGS FGG+P++ QR
Sbjct: 197 LDYIYEENLRRETEFGGSDFGGYPTIAQR 225
>ref|XP_004146603.1| PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein
LOC101209711 [Cucumis sativus]
Length = 581
Score = 127 bits (320), Expect = 1e-27
Identities = 64/113 (56%), Positives = 79/113 (69%), Gaps = 8/113 (7%)
Frame = +1
Query: 46 SSSHSMSVSGKNKSRRSPHP-------VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPV 204
+SS SM+ + S P P +G TLPPGHPC +F+L PPP DKKRTGPRPCPV
Sbjct: 111 NSSSSMAKGIETSSLAPPPPPPPPASALGYTLPPGHPCNNFALPPPPADKKRTGPRPCPV 170
Query: 205 CYLPVEKAMVSMPAVTSLSPVLRNLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
CYLPVE+A+ MP +S SPVL+ L Y+ E+N R TEFGGS FGG+P++ QR
Sbjct: 171 CYLPVEEAVALMPNASSDSPVLKKLDYIYEENLRRETEFGGSDFGGYPTIAQR 223
>ref|XP_006415681.1| hypothetical protein EUTSA_v10007204mg [Eutrema salsugineum]
gi|557093452|gb|ESQ34034.1| hypothetical protein
EUTSA_v10007204mg [Eutrema salsugineum]
Length = 573
Score = 127 bits (318), Expect = 2e-27
Identities = 69/148 (46%), Positives = 83/148 (56%), Gaps = 28/148 (18%)
Frame = +1
Query: 1 LLAAKEQDDRKVYIPSSSHSMSVSGKNKSRRSP--------------------------- 99
L K +D ++V +P S H S N S P
Sbjct: 75 LYVGKGEDGQEVQVPPSLHFNGSSVVNHSNMLPTNEDQNINIRNISFLGTNVLVSPTPPM 134
Query: 100 HPVGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNL 279
H +G TLP GHPC F+L PPP D+KRTGPRPCPVCYLPVE+A+ MP S SPVL+NL
Sbjct: 135 HFLGYTLPQGHPCNSFTLPPPPADRKRTGPRPCPVCYLPVEEAIALMPNAPSFSPVLKNL 194
Query: 280 TYV-QEKNPRVTEFGGSWFGGHPSLKQR 360
TY+ +E R TEFGGS FGG+P+LK R
Sbjct: 195 TYIYEEPLNRETEFGGSDFGGYPTLKHR 222
>ref|XP_002509470.1| conserved hypothetical protein [Ricinus communis]
gi|223549369|gb|EEF50857.1| conserved hypothetical
protein [Ricinus communis]
Length = 587
Score = 127 bits (318), Expect = 2e-27
Identities = 59/90 (65%), Positives = 70/90 (77%), Gaps = 1/90 (1%)
Frame = +1
Query: 94 SPHPVGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLR 273
+P+ +G TLPPGHPC F+L PPP DKKRTGPRPCPVCYLPVE+A+ MP S SPV++
Sbjct: 139 TPYFLGYTLPPGHPCNSFTLPPPPADKKRTGPRPCPVCYLPVEEAIALMPKFPSYSPVVK 198
Query: 274 NLTYVQEKN-PRVTEFGGSWFGGHPSLKQR 360
NLTY+ E R EFGGS FGG+P+LKQR
Sbjct: 199 NLTYIYEDPLSRDGEFGGSDFGGYPTLKQR 228
>ref|XP_006583820.1| PREDICTED: uncharacterized protein LOC100783387 isoform X2 [Glycine
max]
Length = 564
Score = 126 bits (317), Expect = 3e-27
Identities = 61/86 (70%), Positives = 69/86 (80%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G LPPGHPC F+L PPP DKKRTGPRPCPVCYLPVE+A+ MPA+ S SPVL NLTY
Sbjct: 126 LGYHLPPGHPCNSFTLPPPPADKKRTGPRPCPVCYLPVEEAIGLMPALPSPSPVLGNLTY 185
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
V E+N R EFGGS FGG+P+LKQR
Sbjct: 186 VYEENLSRDGEFGGSDFGGYPTLKQR 211
>ref|XP_006583819.1| PREDICTED: uncharacterized protein LOC100783387 isoform X1 [Glycine
max]
Length = 590
Score = 126 bits (317), Expect = 3e-27
Identities = 61/86 (70%), Positives = 69/86 (80%), Gaps = 1/86 (1%)
Frame = +1
Query: 106 VGSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTY 285
+G LPPGHPC F+L PPP DKKRTGPRPCPVCYLPVE+A+ MPA+ S SPVL NLTY
Sbjct: 126 LGYHLPPGHPCNSFTLPPPPADKKRTGPRPCPVCYLPVEEAIGLMPALPSPSPVLGNLTY 185
Query: 286 VQEKN-PRVTEFGGSWFGGHPSLKQR 360
V E+N R EFGGS FGG+P+LKQR
Sbjct: 186 VYEENLSRDGEFGGSDFGGYPTLKQR 211
>ref|XP_006343239.1| PREDICTED: uncharacterized protein LOC102601452 [Solanum tuberosum]
Length = 580
Score = 126 bits (316), Expect = 4e-27
Identities = 57/84 (67%), Positives = 66/84 (78%)
Frame = +1
Query: 109 GSTLPPGHPCQHFSLTPPPTDKKRTGPRPCPVCYLPVEKAMVSMPAVTSLSPVLRNLTYV 288
G TLPPG+PC+ F+L PPP DKKRTGPRPCPVCYLPVE+A+ MP S SP + NLTY+
Sbjct: 140 GYTLPPGNPCEGFTLPPPPADKKRTGPRPCPVCYLPVEQAIALMPDAPSFSPGVSNLTYI 199
Query: 289 QEKNPRVTEFGGSWFGGHPSLKQR 360
E+N TEFGGS FGG+PSL QR
Sbjct: 200 HEENQSKTEFGGSDFGGYPSLIQR 223