BLASTX nr result
ID: Sinomenium21_contig00035874
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium21_contig00035874
(761 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana] 297 2e-78
gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana] 295 2e-77
gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica] 291 2e-76
sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName... 290 5e-76
pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 288 1e-75
gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera] 287 2e-75
dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica] 285 2e-74
gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp.... 283 6e-74
pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a ... 281 2e-73
pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 281 2e-73
pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Comp... 280 4e-73
gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver so... 278 1e-72
pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a ... 277 3e-72
pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ... 276 4e-72
pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a ... 276 4e-72
sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName... 276 6e-72
gb|ACO90226.1| putative berberine bridge enzyme [Papaver bractea... 273 5e-71
gb|ACO90228.1| berberine bridge enzyme-like protein [Papaver bra... 263 4e-68
ref|XP_007099511.1| FAD-binding Berberine family protein [Theobr... 239 6e-61
ref|XP_003555533.1| PREDICTED: reticuline oxidase-like [Glycine ... 239 6e-61
>gb|ACJ76784.1| berberine bridge enzyme 2 [Argemone mexicana]
Length = 536
Score = 297 bits (761), Expect = 2e-78
Identities = 146/218 (66%), Positives = 176/218 (80%), Gaps = 1/218 (0%)
Frame = +2
Query: 110 SFLCVFLSILTNTLAKEDLLSCLSSNSVHNYTT-FSNSNTDYHRLLNLVIQNPMFTRPTF 286
S L +F S+LT L+ +D+LSCL+SN VHNYTT S+SN+DY RL +L IQNP+F + T
Sbjct: 17 SSLFIFFSVLTCALS-DDILSCLTSNGVHNYTTPSSDSNSDYLRLFHLSIQNPLFKKSTI 75
Query: 287 NKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRI 466
+KPS IVLP NKE+L+N + CC RGSWTIR+RSGGHSYEGLS+ ADTPFVLIDLMNLNRI
Sbjct: 76 SKPSLIVLPGNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLNRI 135
Query: 467 EIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYG 646
ID++SETAWVESGAT+GE+YYAIS+++DS GF+AG+CPTV RKYG
Sbjct: 136 SIDMDSETAWVESGATVGELYYAISESTDSFGFTAGWCPTVGTGGHISGGGFGMMSRKYG 195
Query: 647 LAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
LAADNV D +LID+NG ILDR+ MGEDVFWA+RGGGGG
Sbjct: 196 LAADNVEDVILIDSNGAILDRKLMGEDVFWAVRGGGGG 233
>gb|ACJ76783.1| berberine bridge enzyme 1 [Argemone mexicana]
Length = 554
Score = 295 bits (754), Expect = 2e-77
Identities = 147/228 (64%), Positives = 176/228 (77%), Gaps = 1/228 (0%)
Frame = +2
Query: 80 TMKPYHRVTISFLCVFLSILTNTLAKEDLLSCLSSNSVHNYTT-FSNSNTDYHRLLNLVI 256
T Y + I F S+LT L+ +D+LSCL+SN VHNYTT S+SN+DY RL +L I
Sbjct: 12 TKNLYSSLFIIIFFFFFSVLTCALS-DDILSCLTSNGVHNYTTPSSDSNSDYLRLYHLSI 70
Query: 257 QNPMFTRPTFNKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFV 436
QNP+F + T +KPS IVLP NKE+L+N + CC RGSWTIR+RSGGHSYEGLS+ ADTPFV
Sbjct: 71 QNPLFEKSTISKPSLIVLPRNKEELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFV 130
Query: 437 LIDLMNLNRIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXX 616
LIDLMNLNRI ID++SETAWVESGATLGE+YYAI++ +DSLGF+AG+CPTV
Sbjct: 131 LIDLMNLNRISIDIDSETAWVESGATLGELYYAITELTDSLGFTAGWCPTVGSGGHISGG 190
Query: 617 XXXXXXRKYGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
RKYGLAADNV D +LID+ G ILDR+ MGEDVFWA+RGGGGG
Sbjct: 191 GFGMMSRKYGLAADNVEDVILIDSKGAILDRKLMGEDVFWAVRGGGGG 238
>gb|ACO90218.1| berberine bridge enzyme [Eschscholzia californica]
Length = 242
Score = 291 bits (744), Expect = 2e-76
Identities = 140/216 (64%), Positives = 174/216 (80%), Gaps = 1/216 (0%)
Frame = +2
Query: 116 LCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNK 292
L +FLS+L L DLLSCL+ N V N+T FS +S++D++RLL+L IQNP+F +K
Sbjct: 11 LSIFLSLLNCALGGNDLLSCLTFNGVRNHTVFSADSDSDFNRLLHLSIQNPLFQNSLISK 70
Query: 293 PSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEI 472
PSAI+LP +KE+L+N I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+L+DLMNLNR+ I
Sbjct: 71 PSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILVDLMNLNRVSI 130
Query: 473 DLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLA 652
DLESETAWVESG+TLGE+YYAI+++S LGF+AG+CPTV RKYGLA
Sbjct: 131 DLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLA 190
Query: 653 ADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
ADNV+DA+LIDANG ILDR++MGEDVFWAIRGGGGG
Sbjct: 191 ADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGG 226
>sp|P30986.1|RETO_ESCCA RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|239110|gb|AAB20352.1| (S)-reticuline:oxygen
oxidoreductase (methylene-bridge-forming) [Eschscholzia
californica] gi|2897944|gb|AAC39358.1| berberine bridge
enzyme [Eschscholzia californica]
Length = 538
Score = 290 bits (741), Expect = 5e-76
Identities = 140/216 (64%), Positives = 173/216 (80%), Gaps = 1/216 (0%)
Frame = +2
Query: 116 LCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNK 292
L +FLS+L L DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +K
Sbjct: 11 LSIFLSLLNCALGGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISK 70
Query: 293 PSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEI 472
PSAI+LP +KE+L+N I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ I
Sbjct: 71 PSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSI 130
Query: 473 DLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLA 652
DLESETAWVESG+TLGE+YYAI+++S LGF+AG+CPTV RKYGLA
Sbjct: 131 DLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLA 190
Query: 653 ADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
ADNV+DA+LIDANG ILDR++MGEDVFWAIRGGGGG
Sbjct: 191 ADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGG 226
>pdb|3D2D|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Reticuline gi|210060956|pdb|3D2H|A Chain A,
Structure Of Berberine Bridge Enzyme From Eschscholzia
Californica, Monoclinic Crystal Form
gi|210060957|pdb|3D2J|A Chain A, Structure Of Berberine
Bridge Enzyme From Eschscholzia Californica, Tetragonal
Crystal Form
Length = 538
Score = 288 bits (738), Expect = 1e-75
Identities = 140/216 (64%), Positives = 173/216 (80%), Gaps = 1/216 (0%)
Frame = +2
Query: 116 LCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNK 292
L +FLS+L A DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +K
Sbjct: 11 LSIFLSLLNCAEAGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISK 70
Query: 293 PSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEI 472
PSAI+LP +KE+L+N I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ I
Sbjct: 71 PSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSI 130
Query: 473 DLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLA 652
DLESETAWVESG+TLGE+YYAI+++S LGF+AG+CPTV RKYGLA
Sbjct: 131 DLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLA 190
Query: 653 ADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
ADNV+DA+LIDANG ILDR++MGEDVFWAIRGGGGG
Sbjct: 191 ADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGG 226
>gb|AAD17487.1| berberine bridge enzyme [Berberis stolonifera]
Length = 536
Score = 287 bits (735), Expect = 2e-75
Identities = 141/220 (64%), Positives = 172/220 (78%), Gaps = 1/220 (0%)
Frame = +2
Query: 104 TISFLCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRP 280
T F V + +T TL +L SCL+SN V N+T S +S++DYHRLL + +QN +FTRP
Sbjct: 3 TALFFLVTICTITCTLGDVNLSSCLTSNGVSNFTALSTSSDSDYHRLLYVSMQNQIFTRP 62
Query: 281 TFNKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLN 460
+ +PS I+LP +KE+LA +++C RG WTIR+RSGGHSYEGLS++ADTPFV+IDLMNLN
Sbjct: 63 KYPRPSMIILPQSKEELAASVVCSNRGLWTIRLRSGGHSYEGLSYVADTPFVVIDLMNLN 122
Query: 461 RIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRK 640
RI IDLES+TAWVESGATLGEIY AIS+ASD+LGFS GYCPTV RK
Sbjct: 123 RISIDLESKTAWVESGATLGEIYCAISEASDTLGFSGGYCPTVGSGGHISGGGFGMMSRK 182
Query: 641 YGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
YGLAADNV+DA+++DANG +LDR SMGEDVFWAIRGGGGG
Sbjct: 183 YGLAADNVIDALIVDANGAVLDRSSMGEDVFWAIRGGGGG 222
>dbj|BAM44344.1| berberine bridge enzyme [Coptis japonica]
Length = 533
Score = 285 bits (728), Expect = 2e-74
Identities = 139/226 (61%), Positives = 171/226 (75%)
Frame = +2
Query: 83 MKPYHRVTISFLCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFSNSNTDYHRLLNLVIQN 262
M+ H + + + +FL LT + +++DL SCL + V NYTT SN+DY RLL + +QN
Sbjct: 1 MRATHTIISTLMLLFLCNLTCSQSEDDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQN 60
Query: 263 PMFTRPTFNKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLI 442
+FTR TF +PS I+LP +K+QL N I CCRRGSWTIR+RSGGHSYEGLSH AD+PFV+I
Sbjct: 61 QIFTRSTFPRPSVIILPESKDQLVNTISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVII 120
Query: 443 DLMNLNRIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXX 622
D+MNLN + IDL+++TAW ESGATLGEIY+AI +SD LGFSAGYCPTV
Sbjct: 121 DMMNLNGVSIDLDTQTAWAESGATLGEIYHAIGVSSDVLGFSAGYCPTVGSGGHISGGGF 180
Query: 623 XXXXRKYGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
RKYGLAADNV+DA+LI ANG + DR+SMGEDVFWAIRGGGGG
Sbjct: 181 GMMSRKYGLAADNVVDAILISANGALYDRKSMGEDVFWAIRGGGGG 226
>gb|AAU20769.1| berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
Length = 535
Score = 283 bits (723), Expect = 6e-74
Identities = 142/220 (64%), Positives = 169/220 (76%), Gaps = 2/220 (0%)
Frame = +2
Query: 107 ISFLCVFLSILTNTLAKEDLLSCLSSNSVHNYTTF-SNSNTD-YHRLLNLVIQNPMFTRP 280
I L VFL+ T + +DL SCL+ + VHNYTT S SN+D YHRLL + +QN +FTR
Sbjct: 4 ICILLVFLNNFTCAIIDDDLPSCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRS 63
Query: 281 TFNKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLN 460
TF +P I+LP + +QLAN I CC RGSWTIR+RSGGHSYEGLSHIAD PFV+IDLMNLN
Sbjct: 64 TFPQPRVIILPESMDQLANVISCCTRGSWTIRLRSGGHSYEGLSHIADNPFVIIDLMNLN 123
Query: 461 RIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRK 640
I IDL+++TAWVESGATLGEIY+AI ++S ++ FSAGYCPT RK
Sbjct: 124 GISIDLDTQTAWVESGATLGEIYHAIGKSSGTMAFSAGYCPTGGSGGHIAPGGFGMMSRK 183
Query: 641 YGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
YGLAADNV+DA+L+DANG +LDRESMGEDVFWAIRGGGGG
Sbjct: 184 YGLAADNVVDALLVDANGVVLDRESMGEDVFWAIRGGGGG 223
>pdb|4EC3|A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
Complex With (S)-Reticuline
Length = 519
Score = 281 bits (718), Expect = 2e-73
Identities = 135/204 (66%), Positives = 166/204 (81%), Gaps = 1/204 (0%)
Frame = +2
Query: 152 AKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQ 328
A DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +KPSAI+LP +KE+
Sbjct: 4 AGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEE 63
Query: 329 LANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESG 508
L+N I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ IDLESETAWVESG
Sbjct: 64 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 123
Query: 509 ATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDA 688
+TLGE+YYAI+++S LGF+AG+CPTV RKYGLAADNV+DA+LIDA
Sbjct: 124 STLGELYYAITESSSKLGFTAGWCPTVGTGGAISGGGFGMMSRKYGLAADNVVDAILIDA 183
Query: 689 NGNILDRESMGEDVFWAIRGGGGG 760
NG ILDR++MGEDVFWAIRGGGGG
Sbjct: 184 NGAILDRQAMGEDVFWAIRGGGGG 207
>pdb|3GSY|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
Dehydroscoulerine
Length = 519
Score = 281 bits (718), Expect = 2e-73
Identities = 135/204 (66%), Positives = 166/204 (81%), Gaps = 1/204 (0%)
Frame = +2
Query: 152 AKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQ 328
A DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +KPSAI+LP +KE+
Sbjct: 4 AGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEE 63
Query: 329 LANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESG 508
L+N I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ IDLESETAWVESG
Sbjct: 64 LSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 123
Query: 509 ATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDA 688
+TLGE+YYAI+++S LGF+AG+CPTV RKYGLAADNV+DA+LIDA
Sbjct: 124 STLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDA 183
Query: 689 NGNILDRESMGEDVFWAIRGGGGG 760
NG ILDR++MGEDVFWAIRGGGGG
Sbjct: 184 NGAILDRQAMGEDVFWAIRGGGGG 207
>pdb|3FW9|A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Scoulerine
Length = 495
Score = 280 bits (716), Expect = 4e-73
Identities = 134/201 (66%), Positives = 165/201 (82%), Gaps = 1/201 (0%)
Frame = +2
Query: 161 DLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQLAN 337
DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +KPSAI+LP +KE+L+N
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 338 AILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESGATL 517
I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ IDLESETAWVESG+TL
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTL 120
Query: 518 GEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDANGN 697
GE+YYAI+++S LGF+AG+CPTV RKYGLAADNV+DA+LIDANG
Sbjct: 121 GELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGA 180
Query: 698 ILDRESMGEDVFWAIRGGGGG 760
ILDR++MGEDVFWAIRGGGGG
Sbjct: 181 ILDRQAMGEDVFWAIRGGGGG 201
>gb|AGL44333.1| FAD-dependent oxidoreductase, partial [Papaver somniferum]
Length = 535
Score = 278 bits (712), Expect = 1e-72
Identities = 131/200 (65%), Positives = 165/200 (82%), Gaps = 1/200 (0%)
Frame = +2
Query: 164 LLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQLANA 340
L SCL+S+ VHN+TT S ++N+DY +LL+ IQNP+F +PT +KPS IV+P +KE+L+
Sbjct: 31 LSSCLNSHGVHNFTTLSTDTNSDYFKLLHASIQNPLFAKPTVSKPSFIVMPGSKEELSRT 90
Query: 341 ILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESGATLG 520
+ CC R SWTIR+RSGGHSYEGLS+ ADTPFV++D+MNLNRI ID+ESETAWVESGATLG
Sbjct: 91 VHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLNRISIDVESETAWVESGATLG 150
Query: 521 EIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDANGNI 700
E+YYAI+Q++++LGF+AG+CPTV RKYGLAADNV+DA+LID+ G+I
Sbjct: 151 ELYYAIAQSTETLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILIDSTGSI 210
Query: 701 LDRESMGEDVFWAIRGGGGG 760
LDRE MGEDVFWAIRGGGGG
Sbjct: 211 LDREKMGEDVFWAIRGGGGG 230
>pdb|3FW7|A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
Length = 498
Score = 277 bits (708), Expect = 3e-72
Identities = 134/204 (65%), Positives = 165/204 (80%), Gaps = 1/204 (0%)
Frame = +2
Query: 152 AKEDLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQ 328
A DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +KPSAI+LP +KE+
Sbjct: 1 AGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEE 60
Query: 329 LANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESG 508
L+N I C R+GSWTIR+RSGG SYEGLS+ +DTPF+LIDLMNLNR+ IDLESETAWVESG
Sbjct: 61 LSNTIRCIRKGSWTIRLRSGGASYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESG 120
Query: 509 ATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDA 688
+TLGE+YYAI+++S LGF+AG+CPTV RKYGLAADNV+DA+LIDA
Sbjct: 121 STLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDA 180
Query: 689 NGNILDRESMGEDVFWAIRGGGGG 760
NG ILDR++MGEDVFWAIRGGGGG
Sbjct: 181 NGAILDRQAMGEDVFWAIRGGGGG 204
>pdb|3FWA|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
Complex With (S)-Reticuline
Length = 497
Score = 276 bits (707), Expect = 4e-72
Identities = 133/201 (66%), Positives = 164/201 (81%), Gaps = 1/201 (0%)
Frame = +2
Query: 161 DLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQLAN 337
DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +KPSAI+LP +KE+L+N
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 338 AILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESGATL 517
I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ IDLESETAWVESG+TL
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTL 120
Query: 518 GEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDANGN 697
GE+YYAI+++S LGF+AG+ PTV RKYGLAADNV+DA+LIDANG
Sbjct: 121 GELYYAITESSSKLGFTAGWAPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGA 180
Query: 698 ILDRESMGEDVFWAIRGGGGG 760
ILDR++MGEDVFWAIRGGGGG
Sbjct: 181 ILDRQAMGEDVFWAIRGGGGG 201
>pdb|3FW8|A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant
Length = 495
Score = 276 bits (707), Expect = 4e-72
Identities = 133/201 (66%), Positives = 164/201 (81%), Gaps = 1/201 (0%)
Frame = +2
Query: 161 DLLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQLAN 337
DLLSCL+ N V N+T FS +S++D++R L+L IQNP+F +KPSAI+LP +KE+L+N
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 338 AILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESGATL 517
I C R+GSWTIR+RSGGHSYEGLS+ +DTPF+LIDLMNLNR+ IDLESETAWVESG+TL
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTL 120
Query: 518 GEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDANGN 697
GE+YYAI+++S LGF+AG+ PTV RKYGLAADNV+DA+LIDANG
Sbjct: 121 GELYYAITESSSKLGFTAGWAPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGA 180
Query: 698 ILDRESMGEDVFWAIRGGGGG 760
ILDR++MGEDVFWAIRGGGGG
Sbjct: 181 ILDRQAMGEDVFWAIRGGGGG 201
>sp|P93479.1|RETO_PAPSO RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
gi|3282517|gb|AAC61839.1| berberine bridge enzyme
[Papaver somniferum]
Length = 535
Score = 276 bits (706), Expect = 6e-72
Identities = 130/200 (65%), Positives = 165/200 (82%), Gaps = 1/200 (0%)
Frame = +2
Query: 164 LLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQLANA 340
L SCL+S+ VHN+TT S ++N+DY +LL+ +QNP+F +PT +KPS IV+P +KE+L++
Sbjct: 31 LSSCLNSHGVHNFTTLSTDTNSDYFKLLHASMQNPLFAKPTVSKPSFIVMPGSKEELSST 90
Query: 341 ILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESGATLG 520
+ CC R SWTIR+RSGGHSYEGLS+ ADTPFV++D+MNLNRI ID+ SETAWVESGATLG
Sbjct: 91 VHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLNRISIDVLSETAWVESGATLG 150
Query: 521 EIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDANGNI 700
E+YYAI+Q++D+LGF+AG+CPTV RKYGLAADNV+DA+LID+NG I
Sbjct: 151 ELYYAIAQSTDTLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILIDSNGAI 210
Query: 701 LDRESMGEDVFWAIRGGGGG 760
LDRE MG+DVFWAIRGGGGG
Sbjct: 211 LDREKMGDDVFWAIRGGGGG 230
>gb|ACO90226.1| putative berberine bridge enzyme [Papaver bracteatum]
Length = 259
Score = 273 bits (698), Expect = 5e-71
Identities = 130/200 (65%), Positives = 164/200 (82%), Gaps = 1/200 (0%)
Frame = +2
Query: 164 LLSCLSSNSVHNYTTFS-NSNTDYHRLLNLVIQNPMFTRPTFNKPSAIVLPYNKEQLANA 340
LLSCL+S+ VHN+TT S ++N++Y +LL+ IQNP+F +PT +KPS IV+P +KE+L++
Sbjct: 22 LLSCLNSHDVHNFTTLSTDTNSEYLKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELSST 81
Query: 341 ILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLMNLNRIEIDLESETAWVESGATLG 520
I CC R SWTIR+RSGGHSYEGLS+ ADTPFV++D+MNL+ I ID ESETAWVESGATLG
Sbjct: 82 IHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLHGISIDFESETAWVESGATLG 141
Query: 521 EIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXXXRKYGLAADNVLDAVLIDANGNI 700
E+YYAI+Q++++LGF+AG+CPTV RKYGLAADNV+DA+LID+ G I
Sbjct: 142 ELYYAIAQSTETLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILIDSKGAI 201
Query: 701 LDRESMGEDVFWAIRGGGGG 760
LDRE MGEDVFWAIRGGGGG
Sbjct: 202 LDREKMGEDVFWAIRGGGGG 221
>gb|ACO90228.1| berberine bridge enzyme-like protein [Papaver bracteatum]
Length = 245
Score = 263 bits (673), Expect = 4e-68
Identities = 130/223 (58%), Positives = 169/223 (75%), Gaps = 4/223 (1%)
Frame = +2
Query: 104 TISFLCVFLSILTNTLAKEDLL-SCLSSNSVHNYTT-FSNSNTDYHRLLNLVIQNPMFTR 277
TI F+ +L ++ +D+L SCL+S+ V NYT S+ N+DY RL ++ I NP+F +
Sbjct: 16 TILFISCYLQLICALGGNDDILISCLASHGVENYTKPSSDPNSDYDRLFHINILNPLFKK 75
Query: 278 PTFN--KPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIADTPFVLIDLM 451
T KPS IV+P +K++L++ I CC R SWTI++RSGGHSYEGLS+I+DTPFVL+D+M
Sbjct: 76 TTSERLKPSLIVMPNSKQELSSTIKCCTRSSWTIKLRSGGHSYEGLSYISDTPFVLVDMM 135
Query: 452 NLNRIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXX 631
NL+RI IDL SETAWVESGAT+G++YYAI +++DS GF+AG+CP V
Sbjct: 136 NLDRISIDLNSETAWVESGATIGQLYYAIHESTDSYGFTAGWCPNVGVGGHLSGGGLGML 195
Query: 632 XRKYGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
RKYGLAADNV+DA+LID+NG ILDR+SMGEDVFWAIRGGGGG
Sbjct: 196 SRKYGLAADNVVDAILIDSNGAILDRQSMGEDVFWAIRGGGGG 238
>ref|XP_007099511.1| FAD-binding Berberine family protein [Theobroma cacao]
gi|508728622|gb|EOY20519.1| FAD-binding Berberine family
protein [Theobroma cacao]
Length = 875
Score = 239 bits (611), Expect = 6e-61
Identities = 124/224 (55%), Positives = 156/224 (69%), Gaps = 6/224 (2%)
Frame = +2
Query: 107 ISFLCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFSNSNTD----YHRLLNLVIQNPMFT 274
ISF+ LS+ T E + SCL+ ++++NYTTFSN + Y+ LLN IQN F
Sbjct: 55 ISFVIPALSVSAPT---EIITSCLNRHNINNYTTFSNLENNESSLYYNLLNFSIQNLRFA 111
Query: 275 RPTFNKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIAD--TPFVLIDL 448
T KP AI+LP +KEQL N++LCC G W IR+R GGH+YEG S +A PFV+ID+
Sbjct: 112 EATNPKPIAIILPESKEQLINSVLCCIAGPWEIRVRCGGHNYEGTSSVASDGAPFVIIDM 171
Query: 449 MNLNRIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXX 628
MNLN + +DLESETAWVE GATLGE Y+AI+++S FSAG CPTV
Sbjct: 172 MNLNLVSVDLESETAWVEGGATLGETYHAIAESSRIHAFSAGSCPTVGAGGHIGGGGFGF 231
Query: 629 XXRKYGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
RKYGLAADNV+DA+L++A+G ILDR++MGEDVFWAIRGGGGG
Sbjct: 232 LSRKYGLAADNVIDALLLNADGEILDRQAMGEDVFWAIRGGGGG 275
>ref|XP_003555533.1| PREDICTED: reticuline oxidase-like [Glycine max]
Length = 550
Score = 239 bits (611), Expect = 6e-61
Identities = 122/223 (54%), Positives = 149/223 (66%), Gaps = 8/223 (3%)
Frame = +2
Query: 116 LCVFLSILTNTLAKEDLLSCLSSNSVHNYTTFSNSNTD------YHRLLNLVIQNPMFTR 277
L F L + + L SCL ++++ N+TTF D Y ++LN IQN F
Sbjct: 15 LLSFAVSLFSCASLRGLASCLDNHNIKNFTTFPYKEHDHSSAYSYFKILNFSIQNLRFAE 74
Query: 278 PTFNKPSAIVLPYNKEQLANAILCCRRGSWTIRIRSGGHSYEGLSHIAD--TPFVLIDLM 451
P KP AIVLP + EQL ++ CCR GS IR+R GGHSYEG S++AD TPFV+ID+M
Sbjct: 75 PVIPKPIAIVLPESLEQLQKSVACCREGSMEIRVRCGGHSYEGTSYVADDGTPFVIIDMM 134
Query: 452 NLNRIEIDLESETAWVESGATLGEIYYAISQASDSLGFSAGYCPTVXXXXXXXXXXXXXX 631
NLN + +D+E+ETAWVE GATLGE YYAISQ S+ GFS G CPTV
Sbjct: 135 NLNHVWVDMETETAWVEGGATLGETYYAISQESNEHGFSGGSCPTVGVGGHIGGGGFGLL 194
Query: 632 XRKYGLAADNVLDAVLIDANGNILDRESMGEDVFWAIRGGGGG 760
RKYGLAADNV+DA+L+DANG + DRE+MGEDVFWAIRGGGGG
Sbjct: 195 SRKYGLAADNVVDALLVDANGKLFDRETMGEDVFWAIRGGGGG 237