BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sinomenium21_contig00027978
         (311 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006466653.1| PREDICTED: pentatricopeptide repeat-containi...   162   4e-38
ref|XP_006425825.1| hypothetical protein CICLE_v10024955mg [Citr...   162   4e-38
ref|XP_002324099.1| hypothetical protein POPTR_0017s12720g [Popu...   159   3e-37
ref|XP_003635569.1| PREDICTED: pentatricopeptide repeat-containi...   155   4e-36
ref|XP_003632466.1| PREDICTED: pentatricopeptide repeat-containi...   155   4e-36
emb|CBI25911.3| unnamed protein product [Vitis vinifera]              155   4e-36
ref|XP_004511479.1| PREDICTED: LOW QUALITY PROTEIN: pentatricope...   154   9e-36
gb|EYU39396.1| hypothetical protein MIMGU_mgv1a026743mg, partial...   154   2e-35
ref|XP_007047157.1| Pentatricopeptide repeat (PPR) superfamily p...   154   2e-35
ref|XP_007150279.1| hypothetical protein PHAVU_005G140500g [Phas...   153   2e-35
ref|XP_007203614.1| hypothetical protein PRUPE_ppa002292mg [Prun...   152   5e-35
ref|XP_003542017.1| PREDICTED: pentatricopeptide repeat-containi...   151   1e-34
ref|XP_004301849.1| PREDICTED: pentatricopeptide repeat-containi...   148   7e-34
gb|EXC24858.1| hypothetical protein L484_013224 [Morus notabilis]     147   2e-33
ref|XP_004229908.1| PREDICTED: pentatricopeptide repeat-containi...   144   1e-32
ref|XP_006339636.1| PREDICTED: pentatricopeptide repeat-containi...   143   3e-32
ref|XP_004152308.1| PREDICTED: pentatricopeptide repeat-containi...   142   6e-32
ref|XP_003610927.1| Pentatricopeptide repeat-containing protein ...   142   6e-32
gb|EMT07616.1| hypothetical protein F775_11615 [Aegilops tauschii]    141   8e-32
ref|XP_006651199.1| PREDICTED: pentatricopeptide repeat-containi...   140   1e-31

>ref|XP_006466653.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Citrus sinensis]
          Length = 695

 Score =  162 bits (410), Expect = 4e-38
 Identities = 75/103 (72%), Positives = 87/103 (84%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATYVT+ANIYA+A  W EV  +RK+MDDRGVVK PG SWIE+ R
Sbjct: 511 AKRAAEALFEIEPENPATYVTMANIYASAGKWSEVASIRKLMDDRGVVKKPGLSWIEIQR 570

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
             H FLVGD SHP++KEIH+FL K+S RMKEEGY+P T+FVLH
Sbjct: 571 DAHVFLVGDTSHPRSKEIHEFLGKLSKRMKEEGYVPDTNFVLH 613


>ref|XP_006425825.1| hypothetical protein CICLE_v10024955mg [Citrus clementina]
           gi|557527815|gb|ESR39065.1| hypothetical protein
           CICLE_v10024955mg [Citrus clementina]
          Length = 759

 Score =  162 bits (410), Expect = 4e-38
 Identities = 75/103 (72%), Positives = 87/103 (84%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATYVT+ANIYA+A  W EV  +RK+MDDRGVVK PG SWIE+ R
Sbjct: 575 AKRAAEALFEIEPENPATYVTMANIYASAGKWSEVASIRKLMDDRGVVKKPGLSWIEIQR 634

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
             H FLVGD SHP++KEIH+FL K+S RMKEEGY+P T+FVLH
Sbjct: 635 DAHVFLVGDTSHPRSKEIHEFLGKLSKRMKEEGYVPDTNFVLH 677


>ref|XP_002324099.1| hypothetical protein POPTR_0017s12720g [Populus trichocarpa]
           gi|222867101|gb|EEF04232.1| hypothetical protein
           POPTR_0017s12720g [Populus trichocarpa]
          Length = 676

 Score =  159 bits (403), Expect = 3e-37
 Identities = 75/103 (72%), Positives = 87/103 (84%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A+RAAEALF+IEPEN ATYVTLANIYA A MW EV  +RK MDDRGVVK PG SWI + R
Sbjct: 492 AQRAAEALFEIEPENPATYVTLANIYATAGMWSEVAKIRKTMDDRGVVKKPGLSWIAIKR 551

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
            VH FLVGD+SHPK+KEI++FL K+S RMKEEG++P T+FVLH
Sbjct: 552 DVHVFLVGDDSHPKSKEINEFLGKLSKRMKEEGFVPDTNFVLH 594


>ref|XP_003635569.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like, partial [Vitis vinifera]
          Length = 287

 Score =  155 bits (393), Expect = 4e-36
 Identities = 74/103 (71%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATY TLANIYA A +W  V  +RK+MD RGVVK PG SWIE+ R
Sbjct: 103 AKRAAEALFEIEPENPATYTTLANIYATAGLWGGVAEVRKVMDARGVVKKPGLSWIEIKR 162

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD SH K+KEIH+FL K+S RMKEEGY+P T+FVLH
Sbjct: 163 EVHVFLVGDTSHAKSKEIHEFLGKLSKRMKEEGYVPDTNFVLH 205


>ref|XP_003632466.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like, partial [Vitis vinifera]
          Length = 621

 Score =  155 bits (393), Expect = 4e-36
 Identities = 74/103 (71%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATY TLANIYA A +W  V  +RK+MD RGVVK PG SWIE+ R
Sbjct: 511 AKRAAEALFEIEPENPATYTTLANIYATAGLWGGVAEVRKVMDARGVVKKPGLSWIEIKR 570

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD SH K+KEIH+FL K+S RMKEEGY+P T+FVLH
Sbjct: 571 EVHVFLVGDTSHAKSKEIHEFLGKLSKRMKEEGYVPDTNFVLH 613


>emb|CBI25911.3| unnamed protein product [Vitis vinifera]
          Length = 288

 Score =  155 bits (393), Expect = 4e-36
 Identities = 74/103 (71%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATY TLANIYA A +W  V  +RK+MD RGVVK PG SWIE+ R
Sbjct: 104 AKRAAEALFEIEPENPATYTTLANIYATAGLWGGVAEVRKVMDARGVVKKPGLSWIEIKR 163

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD SH K+KEIH+FL K+S RMKEEGY+P T+FVLH
Sbjct: 164 EVHVFLVGDTSHAKSKEIHEFLGKLSKRMKEEGYVPDTNFVLH 206


>ref|XP_004511479.1| PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing
           protein At4g37170-like [Cicer arietinum]
          Length = 700

 Score =  154 bits (390), Expect = 9e-36
 Identities = 69/103 (66%), Positives = 89/103 (86%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A+RAA+ALF+IEPEN ATY+TLANIYA A +W +V  +RK M++RG+VK PG SWIE+ R
Sbjct: 481 AERAAKALFEIEPENPATYITLANIYANAGLWTKVAKVRKDMENRGIVKKPGKSWIEIKR 540

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD+SHPKT +IH+FL ++ST+MKEEGY+P T+FVLH
Sbjct: 541 QVHVFLVGDKSHPKTSDIHEFLGELSTKMKEEGYVPDTNFVLH 583


>gb|EYU39396.1| hypothetical protein MIMGU_mgv1a026743mg, partial [Mimulus
           guttatus]
          Length = 670

 Score =  154 bits (388), Expect = 2e-35
 Identities = 72/103 (69%), Positives = 86/103 (83%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A+RAAEALFQIEPENAATYVTLANIYA A  W EV  +RK+MD++GVVK PG SWI + +
Sbjct: 486 AERAAEALFQIEPENAATYVTLANIYATAGKWGEVANVRKLMDEKGVVKKPGMSWINIKK 545

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           KVH FLVGD+SH K++EI  FL ++S RMKEEGY+P TD+VLH
Sbjct: 546 KVHVFLVGDQSHLKSREIFDFLGEVSKRMKEEGYVPHTDYVLH 588


>ref|XP_007047157.1| Pentatricopeptide repeat (PPR) superfamily protein [Theobroma
           cacao] gi|508699418|gb|EOX91314.1| Pentatricopeptide
           repeat (PPR) superfamily protein [Theobroma cacao]
          Length = 684

 Score =  154 bits (388), Expect = 2e-35
 Identities = 71/103 (68%), Positives = 87/103 (84%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A++AAEALF+IEPEN ATYVT+ANIYA A  W EV  +RK MDD+GVVK PG SWIEV R
Sbjct: 500 AEKAAEALFEIEPENPATYVTMANIYATAGRWDEVAKIRKKMDDKGVVKKPGLSWIEVKR 559

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           ++H FLVGD +HPK+KEI++FL K+S RM+EEGY+P T+FVLH
Sbjct: 560 ELHVFLVGDTTHPKSKEINEFLVKLSKRMREEGYVPNTNFVLH 602


>ref|XP_007150279.1| hypothetical protein PHAVU_005G140500g [Phaseolus vulgaris]
           gi|561023543|gb|ESW22273.1| hypothetical protein
           PHAVU_005G140500g [Phaseolus vulgaris]
          Length = 681

 Score =  153 bits (387), Expect = 2e-35
 Identities = 70/103 (67%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAA+AL+ IEPEN ATY+TLANIYA A +W EV  +RK MD+RG+VK PG SWIE+ R
Sbjct: 497 AKRAAKALYDIEPENPATYITLANIYANAGLWTEVAKVRKDMDNRGIVKKPGKSWIEIKR 556

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD SHPKT  IH+FL ++S +MKEEGY+P T+FVLH
Sbjct: 557 QVHVFLVGDTSHPKTSHIHEFLVELSKKMKEEGYVPDTNFVLH 599


>ref|XP_007203614.1| hypothetical protein PRUPE_ppa002292mg [Prunus persica]
           gi|462399145|gb|EMJ04813.1| hypothetical protein
           PRUPE_ppa002292mg [Prunus persica]
          Length = 691

 Score =  152 bits (384), Expect = 5e-35
 Identities = 71/103 (68%), Positives = 84/103 (81%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATY+TLANIYA   MW EV  +RK MD+RGV+K PG SWIE+ R
Sbjct: 507 AKRAAEALFEIEPENPATYITLANIYATGGMWDEVTKVRKTMDERGVIKKPGLSWIEIKR 566

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD+SH +  EIH FL ++S RMKEEGY+P T+FVLH
Sbjct: 567 EVHVFLVGDKSHLRYDEIHFFLHELSKRMKEEGYVPDTNFVLH 609


>ref|XP_003542017.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Glycine max]
          Length = 693

 Score =  151 bits (381), Expect = 1e-34
 Identities = 68/103 (66%), Positives = 86/103 (83%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAA+AL++IEPEN ATY+TLANIYA A +W EV  +RK MD+ G+VK PG SWIE+ R
Sbjct: 509 AKRAAKALYEIEPENPATYITLANIYANAGLWSEVANVRKDMDNMGIVKKPGKSWIEIKR 568

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD SHPKT +IH+FL ++S ++KEEGY+P T+FVLH
Sbjct: 569 QVHVFLVGDTSHPKTSDIHEFLGELSKKIKEEGYVPDTNFVLH 611


>ref|XP_004301849.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Fragaria vesca subsp. vesca]
          Length = 757

 Score =  148 bits (374), Expect = 7e-34
 Identities = 70/103 (67%), Positives = 81/103 (78%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF IEPEN ATYVTLANIYA A MW EV  +RK MD+ G+ K PG SWIE+ R
Sbjct: 573 AKRAAEALFVIEPENPATYVTLANIYATAGMWSEVTNVRKKMDESGITKKPGLSWIEIKR 632

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           ++H FLVGD+SHP+  EI  FL ++S RMKEEGY+P T FVLH
Sbjct: 633 EMHVFLVGDQSHPRYNEIDHFLSELSKRMKEEGYVPDTKFVLH 675


>gb|EXC24858.1| hypothetical protein L484_013224 [Morus notabilis]
          Length = 742

 Score =  147 bits (370), Expect = 2e-33
 Identities = 70/103 (67%), Positives = 80/103 (77%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPEN ATYVTL NIYAAA MW EV  +RK MD R V K PG SWIE+ R
Sbjct: 510 AKRAAEALFEIEPENPATYVTLGNIYAAAGMWGEVAKVRKTMDKRKVAKKPGLSWIEIKR 569

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           + H F +GD SHP+  EI  FL+K+S RM+EEGYIP T+FVLH
Sbjct: 570 EKHAFTIGDMSHPRFNEIDMFLQKLSKRMREEGYIPNTNFVLH 612


>ref|XP_004229908.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Solanum lycopersicum]
          Length = 695

 Score =  144 bits (364), Expect = 1e-32
 Identities = 66/103 (64%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPENAATYVT+AN+YA A  W EV  +R++M+++GVVK PG SWI + R
Sbjct: 511 AKRAAEALFEIEPENAATYVTIANVYATAGKWTEVAKIRRVMEEKGVVKKPGISWINLQR 570

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           K + FLVGD+SHP++KEI++FL ++  RMKEEGY+P  D VLH
Sbjct: 571 KDYVFLVGDKSHPRSKEIYEFLGELWRRMKEEGYVPAIDNVLH 613


>ref|XP_006339636.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Solanum tuberosum]
          Length = 695

 Score =  143 bits (360), Expect = 3e-32
 Identities = 66/103 (64%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAAEALF+IEPENAATYVT+AN+YA A  W EV  +R++M+++GVVK PG SWI + R
Sbjct: 511 AKRAAEALFEIEPENAATYVTIANVYATAGKWTEVAKIRQVMEEKGVVKKPGISWINLLR 570

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           K + FLVGD+SHP++KEI++FL ++  RMKEEGY+P  D VLH
Sbjct: 571 KDYVFLVGDKSHPRSKEIYEFLGELWRRMKEEGYVPDIDNVLH 613


>ref|XP_004152308.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Cucumis sativus]
           gi|449484855|ref|XP_004156999.1| PREDICTED:
           pentatricopeptide repeat-containing protein
           At4g37170-like [Cucumis sativus]
          Length = 724

 Score =  142 bits (357), Expect = 6e-32
 Identities = 67/103 (65%), Positives = 83/103 (80%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           AKRAA++LF+IEPEN ATYVTLANIYA+A M  E   +R+ MD RG+VK PG SWIE+ R
Sbjct: 540 AKRAAKSLFEIEPENPATYVTLANIYASAGMRAEEANIRETMDSRGIVKKPGMSWIEIRR 599

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH F VGD SHPK+KEI ++L ++S RMKE GY+P T+FVLH
Sbjct: 600 EVHVFSVGDNSHPKSKEILEYLSELSKRMKEVGYVPDTNFVLH 642


>ref|XP_003610927.1| Pentatricopeptide repeat-containing protein [Medicago truncatula]
           gi|355512262|gb|AES93885.1| Pentatricopeptide
           repeat-containing protein [Medicago truncatula]
          Length = 802

 Score =  142 bits (357), Expect = 6e-32
 Identities = 63/103 (61%), Positives = 83/103 (80%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A+RAA+ALF++EPEN ATY+TL+NIYA A +W E   +R  MD+RG+VK PG SWIE+ R
Sbjct: 485 AERAAKALFELEPENPATYITLSNIYANAGLWTEETKVRNDMDNRGIVKKPGKSWIEIKR 544

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD SHPK  +IH++L ++S +MKEEGY+  T+FVLH
Sbjct: 545 QVHVFLVGDTSHPKISDIHEYLGELSKKMKEEGYVADTNFVLH 587


>gb|EMT07616.1| hypothetical protein F775_11615 [Aegilops tauschii]
          Length = 438

 Score =  141 bits (356), Expect = 8e-32
 Identities = 66/103 (64%), Positives = 85/103 (82%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A+RAAEALF+IEPEN ATYVTLANIYA+  ++ EVE +RKIM+ +G+ K P SSWIEV R
Sbjct: 254 ARRAAEALFEIEPENPATYVTLANIYASVGLFDEVEGVRKIMETKGITKMPASSWIEVGR 313

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD+SHP+T+EI+  L+K+  +M+EEGY+  T FVLH
Sbjct: 314 RVHVFLVGDKSHPRTEEIYALLKKLYGKMREEGYVADTGFVLH 356


>ref|XP_006651199.1| PREDICTED: pentatricopeptide repeat-containing protein
           At4g37170-like [Oryza brachyantha]
          Length = 637

 Score =  140 bits (354), Expect = 1e-31
 Identities = 65/103 (63%), Positives = 84/103 (81%)
 Frame = -2

Query: 310 AKRAAEALFQIEPENAATYVTLANIYAAASMWKEVEMLRKIMDDRGVVKNPGSSWIEVNR 131
           A+RAAE LF+IEPEN ATYVTLANIYA+  ++ EVE +R+IM+ +GV K P SSWIEV R
Sbjct: 453 ARRAAEVLFEIEPENPATYVTLANIYASVGLFDEVEDVRRIMESKGVTKMPASSWIEVGR 512

Query: 130 KVHKFLVGDESHPKTKEIHQFLEKISTRMKEEGYIPKTDFVLH 2
           +VH FLVGD+SHP+  EI+  L+K+S +M+EEGY+  T+FVLH
Sbjct: 513 RVHVFLVGDKSHPQADEIYALLKKLSVKMREEGYVADTEFVLH 555