BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sinomenium21_contig00023086
         (1236 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   405   e-110
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   372   e-100
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   372   e-100
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   372   e-100
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   369   1e-99
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   368   3e-99
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              368   3e-99
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   368   3e-99
emb|CBI34715.3| unnamed protein product [Vitis vinifera]              365   3e-98
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   365   3e-98
ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   364   5e-98
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   363   1e-97
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   363   1e-97
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   360   5e-97
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   359   1e-96
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              358   3e-96
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   358   3e-96
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   358   3e-96
ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   358   3e-96
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   353   8e-95

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  405 bits (1040), Expect = e-110
 Identities = 188/379 (49%), Positives = 279/379 (73%), Gaps = 3/379 (0%)
 Frame = +3

Query: 108  KNLPPGPFSWPLLGTLGL--KSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAA 281
            KNLPPGP  WPL+G L +   + E   H+ L N+A+T+GP+M+L LG +P ++AST +AA
Sbjct: 26   KNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAA 85

Query: 282  MEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLESQA 461
            MEILKT D + S R    S +++ +I+ S+VW+D  ++WK++RKI RTE+FS KML++Q+
Sbjct: 86   MEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQS 145

Query: 462  SIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDT-GMKGM 638
             +RE++V+ELI FLR +EG+VVKIS  +FG ++N+LG+V+F++ V+ +  + D  G++ +
Sbjct: 146  HVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYGDETDKGGIQNL 205

Query: 639  IRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDHSRND 818
            IRE++++ A PN+ +FYP  +  D+QGL++     + R+ + W G VKER+A+R+    D
Sbjct: 206  IREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKMWEGTVKERKANRNEESKD 265

Query: 819  FLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVIG 998
             LDVL+  +F D QI+ALFLE F PGS++++  +EW + EL+++P++  KV+ EL  V+G
Sbjct: 266  MLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVG 325

Query: 999  NRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNAY 1178
                +KESDLP LPYL AC+KE++RLHP   FLLP +A ETC+VM YTIPK +Q++VNAY
Sbjct: 326  T-STIKESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAY 384

Query: 1179 AIGRDPKTWDDPLCFKPER 1235
            AIGRDPK+W DP  F PER
Sbjct: 385  AIGRDPKSWKDPSTFWPER 403


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
            AltName: Full=Cytochrome P450 80B1
            gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  372 bits (955), Expect = e-100
 Identities = 182/386 (47%), Positives = 267/386 (69%), Gaps = 7/386 (1%)
 Frame = +3

Query: 99   SSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDA 278
            S  KNLPPGP  WP++G L L+  EK  H     LAQTYG +  LK+G + V+VAST  A
Sbjct: 23   SGHKNLPPGPKPWPIVGNL-LQLGEKP-HAQFAELAQTYGDIFTLKMGTETVVVASTSSA 80

Query: 279  AMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLESQ 458
            A EILKT D +LS R    S +V+ ++E S+VW+D  E WK +RK+CRTELF+ KM+ESQ
Sbjct: 81   ASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQ 140

Query: 459  ASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDTG---M 629
            A +RE++  E++ +L +++GE VKI +VIFG ++N+ G++IF+Q++++  G  ++G    
Sbjct: 141  AHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFEL-GDPNSGSSEF 199

Query: 630  KGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRD-- 803
            K  +  ++ L  + N  D++P+  +FD+ G R+  + C K +   W  +++ER+ ++   
Sbjct: 200  KEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVD 259

Query: 804  --HSRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQN 977
               S+NDF+DV + +   D QI+AL +E+F  G++++ + +EWAMTEL +NP+   K+++
Sbjct: 260  GYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRS 319

Query: 978  ELKTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGT 1157
            E++TV+G R  VKESD PNLPYL A VKE+LRLHPP   LLP +A ETC ++NYTIPK  
Sbjct: 320  EIQTVVGERS-VKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDC 378

Query: 1158 QLMVNAYAIGRDPKTWDDPLCFKPER 1235
            Q+MVNA+ IGRDPKTW DPL F PER
Sbjct: 379  QIMVNAWGIGRDPKTWTDPLTFSPER 404


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  372 bits (955), Expect = e-100
 Identities = 188/384 (48%), Positives = 270/384 (70%), Gaps = 3/384 (0%)
 Frame = +3

Query: 93   KPSSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTH 272
            + S PKNLPPGP   P++G L L+  +K  H     LAQ YG L  LKLG + V+VAS+ 
Sbjct: 22   RDSRPKNLPPGPRPSPIVGNL-LQLGDKP-HAEFAKLAQKYGELFSLKLGSQTVVVASSP 79

Query: 273  DAAMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLE 452
             AA EILKT D +LSGR    S +V++++E S+VW++ N++WK++RK+CRTELF+ KM+E
Sbjct: 80   AAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIE 139

Query: 453  SQASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKK--DTG 626
            SQ+ IRE +  E++ FLR +EGEVVKI +V+FG ++N+ G++IF++ V+D E        
Sbjct: 140  SQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVE 199

Query: 627  MKGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASR-D 803
            +K  + +L+ +  + N  D++PI  + D+ G RR  +   +++ + W  ++KERR ++  
Sbjct: 200  LKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGS 259

Query: 804  HSRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNEL 983
             S+NDF+DVL+ A   D +I+AL +E+F  G++++ + +EWA+TEL + P    K++ EL
Sbjct: 260  ESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLEL 319

Query: 984  KTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQL 1163
              V+G+   VKESDLP+LPYL A VKE+LRLHPP   LLP +A ETC VMNYTIPK  Q+
Sbjct: 320  VNVVGD-NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQI 378

Query: 1164 MVNAYAIGRDPKTWDDPLCFKPER 1235
            MVNA+AIGRDPKTWDDPL FKPER
Sbjct: 379  MVNAWAIGRDPKTWDDPLNFKPER 402


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
            AltName: Full=Cytochrome P450 80B2
            gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  372 bits (954), Expect = e-100
 Identities = 183/386 (47%), Positives = 267/386 (69%), Gaps = 7/386 (1%)
 Frame = +3

Query: 99   SSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDA 278
            S  KNLPPGP  WP++G L L+  EK  H     LAQTYG +  LK+G + V+VAST  A
Sbjct: 24   SGHKNLPPGPKPWPIVGNL-LQLGEKP-HAQFAELAQTYGDIFTLKMGTETVVVASTSSA 81

Query: 279  AMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLESQ 458
            A EILKT D +LS R    S +V+ ++E S+VW+D  E WK +RK+CRTELF+ KM+ESQ
Sbjct: 82   ASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQ 141

Query: 459  ASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDTG---M 629
            A +RE++  E++ +L +++GE VKI +VIFG ++N+ G++IF+Q++++  G  ++G    
Sbjct: 142  AHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFEL-GXPNSGSSEF 200

Query: 630  KGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRD-- 803
            K  +  ++ L  + N  D++P+  +FD+ G R+  + C K +   W  +++ER+ ++   
Sbjct: 201  KEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVD 260

Query: 804  --HSRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQN 977
               S+NDF+DV + +   D QI+AL +E+F  G++++ + +EWAMTEL +NP+   K+++
Sbjct: 261  GYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRS 320

Query: 978  ELKTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGT 1157
            EL+TV+G R  VKESD PNLPYL A VKE+LRLHPP   LLP +A ETC ++NYTIPK  
Sbjct: 321  ELQTVVGERS-VKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDC 379

Query: 1158 QLMVNAYAIGRDPKTWDDPLCFKPER 1235
            Q+MVNA+ IGRDPKTW DPL F PER
Sbjct: 380  QIMVNAWGIGRDPKTWIDPLTFSPER 405


>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2-like [Solanum tuberosum]
          Length = 486

 Score =  369 bits (947), Expect = 1e-99
 Identities = 185/384 (48%), Positives = 267/384 (69%), Gaps = 4/384 (1%)
 Frame = +3

Query: 96   PSSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHD 275
            P SP NLPPGP  WPLLG +      K  H+ L   A+ YGPL+ +KLG + V+VAS+ D
Sbjct: 26   PKSP-NLPPGPRPWPLLGNI--LQIGKSPHISLAQFAKIYGPLISVKLGTQLVVVASSPD 82

Query: 276  AAMEILKTQDHVLSGRCPPHSVQVEDYI--EASVVWA-DRNEHWKMVRKICRTELFSTKM 446
            +A EILKTQD +LS R  P     E  +  + S+V++ D + HWK +R  CRT LFS K 
Sbjct: 83   SAAEILKTQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNHWKFLRAFCRTHLFSPKA 142

Query: 447  LESQASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDT- 623
            +ESQA++RE++VSE+I FLR R+GE VKIS+++F  ++N LG++ F++ + D + + +T 
Sbjct: 143  VESQAALREKKVSEMIHFLRSRKGETVKISEIMFATILNTLGNLFFSKDLCDLDYETNTS 202

Query: 624  GMKGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRD 803
            G+K +IR+ + L A PN+ +FYP+FD  D+QGLR+ T     ++   W+ IVKE+R +  
Sbjct: 203  GIKHVIRKFVELGAMPNISEFYPLFDALDLQGLRKQTEIYQNQLFNIWSDIVKEKRQAIS 262

Query: 804  HSRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNEL 983
             S +DFLDV+I + F+D Q+D L +E+ + GSD+TT+ +EWAM EL+RN     K+Q EL
Sbjct: 263  RSSSDFLDVMIDSGFSDLQVDILLMELISAGSDTTTSTIEWAMAELLRNKGAMHKLQAEL 322

Query: 984  KTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQL 1163
             + IG   ++ ES++  LPYL ACVKE+LR+HPP  FL+P +A ETC++M+YTIPK ++L
Sbjct: 323  TSKIGENDILTESNITELPYLAACVKETLRIHPPTPFLIPRRAPETCKIMDYTIPKNSRL 382

Query: 1164 MVNAYAIGRDPKTWDDPLCFKPER 1235
             VN +AIGR+  TW+D L F+PER
Sbjct: 383  FVNVWAIGRNSNTWEDALSFRPER 406


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  368 bits (945), Expect = 3e-99
 Identities = 183/386 (47%), Positives = 265/386 (68%), Gaps = 5/386 (1%)
 Frame = +3

Query: 93   KPSSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTH 272
            + SSPK LPPGP  WP++G L L+  EK  H     LA+TYG L  LKLG + V+VAST 
Sbjct: 21   RDSSPKGLPPGPKPWPIVGNL-LQLGEKP-HSQFAELAKTYGDLFTLKLGSETVVVASTP 78

Query: 273  DAAMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLE 452
             AA EILKT D VLSGR    S +V++++E S+VW++ NE WK +RK+CR ELF+ KM+E
Sbjct: 79   LAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIE 138

Query: 453  SQASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDF--EGKKDTG 626
            SQA IRE +  E++ FL+R +G  VKI +V+FG ++N+ G++IF+Q+++    E      
Sbjct: 139  SQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVE 198

Query: 627  MKGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH 806
            MK  +  ++ L  + N  D++P   RFD+ G R+  + C + +   W  ++KER+ ++ H
Sbjct: 199  MKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLH 258

Query: 807  S---RNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQN 977
            +   +NDF+++L+ +   D QI+AL +EIF  G++++ + +EWA++EL +NPE    +++
Sbjct: 259  NNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRS 318

Query: 978  ELKTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGT 1157
            EL +V+G R  VKESD+PN+PYL A VKE+LRLHP    LLP +A ETC+V+NYTIPK  
Sbjct: 319  ELLSVVGKRP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKEC 377

Query: 1158 QLMVNAYAIGRDPKTWDDPLCFKPER 1235
            Q+MVNA+ IGRDPK W DPL F PER
Sbjct: 378  QIMVNAWGIGRDPKRWTDPLKFAPER 403


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  368 bits (944), Expect = 3e-99
 Identities = 185/380 (48%), Positives = 263/380 (69%), Gaps = 6/380 (1%)
 Frame = +3

Query: 114  LPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEIL 293
            LPPGP  WP+LG L    N    H+ L   +Q+YGPL+ L+LG + ++VAST  AAMEIL
Sbjct: 477  LPPGPSPWPILGNLLHLGN--MPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEIL 534

Query: 294  KTQDHVLSGRCPPHSVQVE--DYIEASVVWA-DRNEHWKMVRKICRTELFSTKMLESQAS 464
            KT D VLSGR  PH+V  +  +    S+ WA + N+ WK +R +CR ELFSTK++ESQA 
Sbjct: 535  KTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAW 594

Query: 465  IREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGK-KDTGMKGMI 641
            + E++V E++ F+  +EGEV+K+ +V+F  ++N L +V+ ++    FE   KD GMKG++
Sbjct: 595  VGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLV 654

Query: 642  RELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERR--ASRDHSRN 815
            R++M+  A PNL DFY IF   D+QGL + T     R+   W  +++ERR  AS D S+ 
Sbjct: 655  RKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ 714

Query: 816  DFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVI 995
            DFL++LI++ ++D QI+ LF+E+   G+D++++ +EWAM EL+++PE   KV  EL   I
Sbjct: 715  DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREI 774

Query: 996  GNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNA 1175
             +  + K SDLP+LPYL ACVKE+LRLHP    LLP +A+ +C+VMNYTIPK +Q+ VNA
Sbjct: 775  SDN-LPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNA 833

Query: 1176 YAIGRDPKTWDDPLCFKPER 1235
            +AIGRDP  W+DPL FKPER
Sbjct: 834  WAIGRDPMNWEDPLVFKPER 853


>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2-like [Vitis vinifera]
          Length = 503

 Score =  368 bits (944), Expect = 3e-99
 Identities = 185/380 (48%), Positives = 263/380 (69%), Gaps = 6/380 (1%)
 Frame = +3

Query: 114  LPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEIL 293
            LPPGP  WP+LG L    N    H+ L   +Q+YGPL+ L+LG + ++VAST  AAMEIL
Sbjct: 44   LPPGPSPWPILGNLLHLGN--MPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEIL 101

Query: 294  KTQDHVLSGRCPPHSVQVE--DYIEASVVWA-DRNEHWKMVRKICRTELFSTKMLESQAS 464
            KT D VLSGR  PH+V  +  +    S+ WA + N+ WK +R +CR ELFSTK++ESQA 
Sbjct: 102  KTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAW 161

Query: 465  IREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGK-KDTGMKGMI 641
            + E++V E++ F+  +EGEV+K+ +V+F  ++N L +V+ ++    FE   KD GMKG++
Sbjct: 162  VGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLV 221

Query: 642  RELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERR--ASRDHSRN 815
            R++M+  A PNL DFY IF   D+QGL + T     R+   W  +++ERR  AS D S+ 
Sbjct: 222  RKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ 281

Query: 816  DFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVI 995
            DFL++LI++ ++D QI+ LF+E+   G+D++++ +EWAM EL+++PE   KV  EL   I
Sbjct: 282  DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREI 341

Query: 996  GNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNA 1175
             +  + K SDLP+LPYL ACVKE+LRLHP    LLP +A+ +C+VMNYTIPK +Q+ VNA
Sbjct: 342  SDN-LPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNA 400

Query: 1176 YAIGRDPKTWDDPLCFKPER 1235
            +AIGRDP  W+DPL FKPER
Sbjct: 401  WAIGRDPMNWEDPLVFKPER 420


>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
          Length = 1076

 Score =  365 bits (936), Expect = 3e-98
 Identities = 184/380 (48%), Positives = 262/380 (68%), Gaps = 6/380 (1%)
 Frame = +3

Query: 114  LPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEIL 293
            LPPGP  WP+LG L    N    H+ L   +Q+YGPL+ L+LG + ++VAST  AAMEIL
Sbjct: 608  LPPGPSPWPILGNLLHLGN--MPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEIL 665

Query: 294  KTQDHVLSGRCPPHSVQVE--DYIEASVVWA-DRNEHWKMVRKICRTELFSTKMLESQAS 464
            KT D VLSGR  PH+V  +  +    S+ WA + N  WK +R +CR ELFSTK++ESQA 
Sbjct: 666  KTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAW 725

Query: 465  IREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEG-KKDTGMKGMI 641
            + E++V E++ F+  +EGEV+K+ +V+F  ++N L +V+ ++    FE   KD GMKG++
Sbjct: 726  VGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLV 785

Query: 642  RELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERR--ASRDHSRN 815
            R++++  A PNL DFY IF   D+QGL + T     R+   W  +++ERR  AS D S+ 
Sbjct: 786  RKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ 845

Query: 816  DFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVI 995
            DFL++LI++ ++D QI+ LF+E+   G+D++++ +EWAM EL+++PE   KV  EL   I
Sbjct: 846  DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREI 905

Query: 996  GNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNA 1175
             +  + K SDLP+LPYL ACVKE+LRLHP    LLP +A+ +C+VMNYTIPK +Q+ VNA
Sbjct: 906  SDN-LPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNA 964

Query: 1176 YAIGRDPKTWDDPLCFKPER 1235
            +AIGRDP  W+DPL FKPER
Sbjct: 965  WAIGRDPMNWEDPLVFKPER 984


>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
            [Vitis vinifera]
          Length = 503

 Score =  365 bits (936), Expect = 3e-98
 Identities = 184/380 (48%), Positives = 262/380 (68%), Gaps = 6/380 (1%)
 Frame = +3

Query: 114  LPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEIL 293
            LPPGP  WP+LG L    N    H+ L   +Q+YGPL+ L+LG + ++VAST  AAMEIL
Sbjct: 44   LPPGPSPWPILGNLLHLGN--MPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEIL 101

Query: 294  KTQDHVLSGRCPPHSVQVE--DYIEASVVWA-DRNEHWKMVRKICRTELFSTKMLESQAS 464
            KT D VLSGR  PH+V  +  +    S+ WA + N  WK +R +CR ELFSTK++ESQA 
Sbjct: 102  KTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAW 161

Query: 465  IREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEG-KKDTGMKGMI 641
            + E++V E++ F+  +EGEV+K+ +V+F  ++N L +V+ ++    FE   KD GMKG++
Sbjct: 162  VGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLV 221

Query: 642  RELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERR--ASRDHSRN 815
            R++++  A PNL DFY IF   D+QGL + T     R+   W  +++ERR  AS D S+ 
Sbjct: 222  RKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ 281

Query: 816  DFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVI 995
            DFL++LI++ ++D QI+ LF+E+   G+D++++ +EWAM EL+++PE   KV  EL   I
Sbjct: 282  DFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREI 341

Query: 996  GNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNA 1175
             +  + K SDLP+LPYL ACVKE+LRLHP    LLP +A+ +C+VMNYTIPK +Q+ VNA
Sbjct: 342  SDN-LPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNA 400

Query: 1176 YAIGRDPKTWDDPLCFKPER 1235
            +AIGRDP  W+DPL FKPER
Sbjct: 401  WAIGRDPMNWEDPLVFKPER 420


>ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2-like [Solanum tuberosum]
          Length = 487

 Score =  364 bits (934), Expect = 5e-98
 Identities = 184/381 (48%), Positives = 260/381 (68%), Gaps = 6/381 (1%)
 Frame = +3

Query: 111  NLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEI 290
            NLPPGP  WP+LG +      K  H+ L   A+ +GPL+ +KLG + V+VAS+  +A EI
Sbjct: 30   NLPPGPRPWPVLGNI--LQLGKNPHISLAKFAEIHGPLISVKLGTQLVVVASSPHSAAEI 87

Query: 291  LKTQDHVLSGRCPPHSVQVEDYI--EASVVWA-DRNEHWKMVRKICRTELFSTKMLESQA 461
            LKTQD +LS R PP  V  E  +  + S+V++ D   HWK +R  CRT LFS K +ESQA
Sbjct: 88   LKTQDRLLSARSPPSVVPYELSVIDQHSIVFSSDLGNHWKFLRAFCRTHLFSPKAVESQA 147

Query: 462  SIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVY---DFEGKKDTGMK 632
            ++R+++VSE+I FLR ++GE VKIS+++FG ++N LG++ F++ +    D+E +  +G+K
Sbjct: 148  ALRDKKVSEMIHFLRSKKGETVKISEILFGTILNTLGNLFFSKDLVCGLDYE-RNPSGIK 206

Query: 633  GMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDHSR 812
             + R+ + L A PN+ DFYP+F+  D+QGLR+ T     R+   W+ IVKE+R +     
Sbjct: 207  RVFRKYIELGAMPNISDFYPLFEALDLQGLRKQTEIYQSRLINIWSEIVKEKRQAISRHS 266

Query: 813  NDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTV 992
            +DFLDV+I + F+D QI+ L  E+   GSD+TT+ +EWAMTEL+RN     K+Q EL + 
Sbjct: 267  SDFLDVMIDSGFSDLQINILVTELMGAGSDTTTSTIEWAMTELLRNNGAMHKLQAELTSK 326

Query: 993  IGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVN 1172
             G    + ES++  LPYL ACVKE+LRLH P  FL+P +A ETC++MNYTIPK ++L VN
Sbjct: 327  FGENDTITESNISELPYLAACVKETLRLHSPTPFLIPRRAPETCKIMNYTIPKNSKLFVN 386

Query: 1173 AYAIGRDPKTWDDPLCFKPER 1235
            AYAIGRD  TW+D L FKPER
Sbjct: 387  AYAIGRDSNTWEDALSFKPER 407


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  363 bits (931), Expect = 1e-97
 Identities = 178/386 (46%), Positives = 264/386 (68%), Gaps = 5/386 (1%)
 Frame = +3

Query: 93   KPSSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTH 272
            + SSPK LPPGP  WP++G L L+  EK  H     LA+TYG L  LKLG + V+VAST 
Sbjct: 16   RDSSPKGLPPGPKPWPIVGNL-LQLGEKP-HSQFAQLAETYGDLFSLKLGSETVVVASTP 73

Query: 273  DAAMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLE 452
             AA EILKT D VLSGR    S +V++++E S+VW++ NE WK +RK+CRTELF+ KM+E
Sbjct: 74   LAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIE 133

Query: 453  SQASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDF--EGKKDTG 626
            SQA +RE +  E++ +L++  G  VKI++V+FG ++N+ G++IF+Q+++    E      
Sbjct: 134  SQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVE 193

Query: 627  MKGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH 806
            MK  +  ++ L  + N  D++P   +FD+ G  +  + C + +   W  ++KER+ ++ H
Sbjct: 194  MKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQH 253

Query: 807  S---RNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQN 977
            +   +NDF+++L+ +   D QI+AL +EIF  G++++ + +EWA++EL +NP+    ++ 
Sbjct: 254  NNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRL 313

Query: 978  ELKTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGT 1157
            EL +V+G R  VKESD+PN+PYL A VKE+LRLHP    LLP +A ETC+V+NYTIPK  
Sbjct: 314  ELLSVVGKRP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKEC 372

Query: 1158 QLMVNAYAIGRDPKTWDDPLCFKPER 1235
            Q+MVNA+ IGRDPK W DPL F PER
Sbjct: 373  QIMVNAWGIGRDPKRWTDPLKFSPER 398


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  363 bits (931), Expect = 1e-97
 Identities = 183/379 (48%), Positives = 264/379 (69%), Gaps = 3/379 (0%)
 Frame = +3

Query: 108  KNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAME 287
            KNLPPGP   P++G L    ++ +A      LAQ YG L  LKLG + V+VAS+  AA E
Sbjct: 27   KNLPPGPRPLPIVGNLLQLGDKPRAE--FAKLAQKYGQLFSLKLGSQTVVVASSPAAAAE 84

Query: 288  ILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLESQASI 467
            +LKT D +LSGR    S +V++++E S+VW++ N++WK++RK+CRTELF+ KM+ESQ+ I
Sbjct: 85   VLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 144

Query: 468  REERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKK--DTGMKGMI 641
            RE +  E++ FLR +EGEVVKI +V+FG ++N+ G++IF++ V+D E        +K  +
Sbjct: 145  REGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL 204

Query: 642  RELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASR-DHSRND 818
             +L+ +  + N  D++PI  ++D+ G RR  +   +++ + W   +KERR  +   S ND
Sbjct: 205  WKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNND 264

Query: 819  FLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVIG 998
            F+DVL+ A   D +I+AL +E+F  G++++ + +EWA+TEL + P    K++ EL  V+G
Sbjct: 265  FVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 324

Query: 999  NRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNAY 1178
            +   VKESDLP+LPYL A VKE+LRLHPP   LLP +A ETC VMNYTIPK  Q+MVNA+
Sbjct: 325  D-NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAW 383

Query: 1179 AIGRDPKTWDDPLCFKPER 1235
            AIGRDPKTWDDPL FKPER
Sbjct: 384  AIGRDPKTWDDPLNFKPER 402


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
            glaucum]
          Length = 491

 Score =  360 bits (925), Expect = 5e-97
 Identities = 183/381 (48%), Positives = 264/381 (69%), Gaps = 2/381 (0%)
 Frame = +3

Query: 99   SSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDA 278
            S  K+LPPGP   P++G L L+  EK  H     LA+ YG L  LKLG + V+VAS+  A
Sbjct: 25   SRNKDLPPGPRPSPIVGNL-LQLGEKP-HAEFAKLAEKYGELFTLKLGSQTVVVASSPAA 82

Query: 279  AMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLESQ 458
            A EILKT+D +LSGR    S +V +++  S+VW++ NE+WK++RK+CRTELFS KM+ESQ
Sbjct: 83   AAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQ 142

Query: 459  ASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKK--DTGMK 632
            A IRE +  +++ FLR++E + VKI +V+F  ++N+ G++IF++ V+D E        +K
Sbjct: 143  AYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELK 202

Query: 633  GMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDHSR 812
              + +L+ +  + N  D++PI  +FD+ G RR  +   K++ + W  ++KERR+   H  
Sbjct: 203  EHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGHRE 262

Query: 813  NDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTV 992
            NDF +VL+ A   D +I+AL +E+F  G+++T + +EWA+TEL  N     K++ EL  V
Sbjct: 263  NDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINV 322

Query: 993  IGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVN 1172
            +G++  VKES++P+LPYL A VKE+LRLHPP   LLP +A ETC+VMNYTIPK  Q+MVN
Sbjct: 323  VGHK-TVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVN 381

Query: 1173 AYAIGRDPKTWDDPLCFKPER 1235
            A+AIGRDPKTWDDPL FKPER
Sbjct: 382  AWAIGRDPKTWDDPLTFKPER 402


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  359 bits (922), Expect = 1e-96
 Identities = 176/386 (45%), Positives = 263/386 (68%), Gaps = 5/386 (1%)
 Frame = +3

Query: 93   KPSSPKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTH 272
            + SSPK LPPGP  WP++G L L+  EK  H     LA+TYG L  LKLG + V+VAST 
Sbjct: 16   RDSSPKGLPPGPKPWPIVGNL-LQLGEKP-HSQFAQLAETYGDLFSLKLGSETVVVASTP 73

Query: 273  DAAMEILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLE 452
             AA EILKT D VLSGR    S +V++++E S+VW++ NE WK +RK+CRT+LF+ KM+E
Sbjct: 74   LAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIE 133

Query: 453  SQASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDF--EGKKDTG 626
            SQA +RE +  E++ +L++  G  VKI++V+FG ++N+ G++IF+Q+++    E      
Sbjct: 134  SQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVE 193

Query: 627  MKGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH 806
            MK  +  ++ L  + N  D++P   +FD+ G  +  + C + +   W  ++KE + ++ H
Sbjct: 194  MKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQH 253

Query: 807  S---RNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQN 977
            +   +NDF+++L+ +   D QI+AL +EIF  G++++ + +EWA++EL +NP+    ++ 
Sbjct: 254  NNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRL 313

Query: 978  ELKTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGT 1157
            EL +V+G R  VKESD+PN+PYL A VKE+LRLHP    LLP +A ETC+V+NYTIPK  
Sbjct: 314  ELLSVVGKRP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKEC 372

Query: 1158 QLMVNAYAIGRDPKTWDDPLCFKPER 1235
            Q+MVNA+ IGRDPK W DPL F PER
Sbjct: 373  QIMVNAWGIGRDPKRWTDPLKFSPER 398


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  358 bits (919), Expect = 3e-96
 Identities = 185/384 (48%), Positives = 254/384 (66%), Gaps = 7/384 (1%)
 Frame = +3

Query: 105  PKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAM 284
            P  LPPGP+ WP++G L      K  H  L NLA+ +GPLM L+LG + ++VAS+  AAM
Sbjct: 575  PPPLPPGPYPWPIIGNL--LQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAM 632

Query: 285  EILKTQDHVLSGRCPPHSVQVED--YIEASVVWA-DRNEHWKMVRKICRTELFSTKMLES 455
            E+LKT D  LSGR    SV V++      S+V+A D N +WK +R ICR ELFS K +ES
Sbjct: 633  EVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMES 692

Query: 456  QASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFE--GKKDTGM 629
            Q  +RE +V+EL+ FL  +EGEVVK+ D++F  + N+L +  F+  + DFE  G+    +
Sbjct: 693  QVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGAL 752

Query: 630  KGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH- 806
            K +I +     ATPNL D+YPI    DIQG+ R     ++R+  +W  I+KERR  R + 
Sbjct: 753  KDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNR 812

Query: 807  -SRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNEL 983
             S  DFL+ L++  F D QI+ + LE+F+ G+++++  +EWAM EL+RN +   K++ EL
Sbjct: 813  SSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGEL 872

Query: 984  KTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQL 1163
            + ++G   V +ES LP LPYL ACVKE+LRLHPP   LLPH A ETCQVM YTIPK +Q+
Sbjct: 873  RQIVGESPV-RESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQI 931

Query: 1164 MVNAYAIGRDPKTWDDPLCFKPER 1235
             VN +A+ RDPK WDDPL FKPER
Sbjct: 932  FVNIWAMARDPKIWDDPLSFKPER 955



 Score =  350 bits (898), Expect = 7e-94
 Identities = 179/382 (46%), Positives = 251/382 (65%), Gaps = 5/382 (1%)
 Frame = +3

Query: 105  PKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAM 284
            P  LPPGP+ WP++G L      K  HV L +LA+ +GPLM L+LG + ++VAS+  AA+
Sbjct: 1080 PPPLPPGPYPWPIIGNL--LQLGKNPHVKLASLAKLHGPLMSLRLGTQLMVVASSPAAAL 1137

Query: 285  EILKTQDHVLSGRCPPHSVQVED--YIEASVVWADR-NEHWKMVRKICRTELFSTKMLES 455
            E+LKT D  LSGR    S+ V+D      S+ +A     +WK +R ICRTE+FS K +ES
Sbjct: 1138 EVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMES 1197

Query: 456  QASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDTGMKG 635
               +RE +V EL+ FL  +EGEVVK+ D++F  + N+L +  F+  + DFE +   G+K 
Sbjct: 1198 HVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFERE---GLKD 1254

Query: 636  MIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRD--HS 809
             I     L ATPNL DFYPI D  ++ G ++ +     R+  +W G +KERR  ++   S
Sbjct: 1255 FIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSS 1314

Query: 810  RNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKT 989
              D L+  ++  F D QI+ + LE+F+ G+D++T  +EWA+T+L+RNP+   K+++EL  
Sbjct: 1315 HRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTK 1374

Query: 990  VIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMV 1169
            +IG   V +ES LP+LPYL ACVKE+LRLHPP   LLPH+A ETCQVM YTIPK +Q+ V
Sbjct: 1375 IIGESPV-RESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFV 1433

Query: 1170 NAYAIGRDPKTWDDPLCFKPER 1235
            N +A+GRDPK WDDPL F PER
Sbjct: 1434 NIWAMGRDPKVWDDPLSFTPER 1455



 Score =  335 bits (859), Expect = 2e-89
 Identities = 176/381 (46%), Positives = 246/381 (64%), Gaps = 7/381 (1%)
 Frame = +3

Query: 114  LPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEIL 293
            LPPGP+ WP++G L         HV L NLA+ +GPLM L+LG + ++VAS+  AAME+L
Sbjct: 38   LPPGPYPWPIIGNLLQMGGN--LHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVL 95

Query: 294  KTQDHVLSGRCPPHSVQVEDYIEASVVWADR---NEHWKMVRKICRTELFSTKMLESQAS 464
            KT D  LSGR    ++ V       +  A     N  W+ ++ ICR ELFS K +ES+  
Sbjct: 96   KTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVE 155

Query: 465  IREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDTG--MKGM 638
            +RE +V EL+ FL ++EGEVVK+ D+++  + N+L +  F+    DFEG+   G  +K +
Sbjct: 156  LRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVRGVLLKDL 215

Query: 639  IRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH--SR 812
            + E   L AT N+ DFYPI    DIQG+R+     ++R+  +W  I+KERR  R H  S 
Sbjct: 216  LNENAELGAT-NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSH 274

Query: 813  NDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTV 992
             DFLD L++  F D QI+ + +E+F  G ++++  +EWAM EL++N +   K+ NEL  +
Sbjct: 275  GDFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQI 334

Query: 993  IGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVN 1172
            IG   V +ES LP+LPYL ACVKE+LRLHP    LLPH+ATETCQ+M YTIPK + + VN
Sbjct: 335  IGESPV-RESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVN 393

Query: 1173 AYAIGRDPKTWDDPLCFKPER 1235
             +A+GRDP TW+DPL FKPER
Sbjct: 394  MWAMGRDPGTWEDPLSFKPER 414


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 498

 Score =  358 bits (919), Expect = 3e-96
 Identities = 185/384 (48%), Positives = 254/384 (66%), Gaps = 7/384 (1%)
 Frame = +3

Query: 105  PKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAM 284
            P  LPPGP+ WP++G L      K  H  L NLA+ +GPLM L+LG + ++VAS+  AAM
Sbjct: 35   PPPLPPGPYPWPIIGNL--LQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAM 92

Query: 285  EILKTQDHVLSGRCPPHSVQVED--YIEASVVWA-DRNEHWKMVRKICRTELFSTKMLES 455
            E+LKT D  LSGR    SV V++      S+V+A D N +WK +R ICR ELFS K +ES
Sbjct: 93   EVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMES 152

Query: 456  QASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFE--GKKDTGM 629
            Q  +RE +V+EL+ FL  +EGEVVK+ D++F  + N+L +  F+  + DFE  G+    +
Sbjct: 153  QVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGAL 212

Query: 630  KGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH- 806
            K +I +     ATPNL D+YPI    DIQG+ R     ++R+  +W  I+KERR  R + 
Sbjct: 213  KDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNR 272

Query: 807  -SRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNEL 983
             S  DFL+ L++  F D QI+ + LE+F+ G+++++  +EWAM EL+RN +   K++ EL
Sbjct: 273  SSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGEL 332

Query: 984  KTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQL 1163
            + ++G   V +ES LP LPYL ACVKE+LRLHPP   LLPH A ETCQVM YTIPK +Q+
Sbjct: 333  RQIVGESPV-RESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQI 391

Query: 1164 MVNAYAIGRDPKTWDDPLCFKPER 1235
             VN +A+ RDPK WDDPL FKPER
Sbjct: 392  FVNIWAMARDPKIWDDPLSFKPER 415


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  358 bits (919), Expect = 3e-96
 Identities = 185/384 (48%), Positives = 254/384 (66%), Gaps = 7/384 (1%)
 Frame = +3

Query: 105  PKNLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAM 284
            P  LPPGP+ WP++G L      K  H  L NLA+ +GPLM L+LG + ++VAS+  AAM
Sbjct: 35   PPPLPPGPYPWPIIGNL--LQMGKNPHAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAM 92

Query: 285  EILKTQDHVLSGRCPPHSVQVED--YIEASVVWA-DRNEHWKMVRKICRTELFSTKMLES 455
            E+LKT D  LSGR    SV V++      S+V+A D N +WK +R ICR ELFS K +ES
Sbjct: 93   EVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMES 152

Query: 456  QASIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFE--GKKDTGM 629
            Q  +RE +V+EL+ FL  +EGEVVK+ D++F  + N+L +  F+  + DFE  G+    +
Sbjct: 153  QVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGGAL 212

Query: 630  KGMIRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDH- 806
            K +I +     ATPNL D+YPI    DIQG+ R     ++R+  +W  I+KERR  R + 
Sbjct: 213  KDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNR 272

Query: 807  -SRNDFLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNEL 983
             S  DFL+ L++  F D QI+ + LE+F+ G+++++  +EWAM EL+RN +   K++ EL
Sbjct: 273  SSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGEL 332

Query: 984  KTVIGNRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQL 1163
            + ++G   V +ES LP LPYL ACVKE+LRLHPP   LLPH A ETCQVM YTIPK +Q+
Sbjct: 333  RQIVGESPV-RESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQI 391

Query: 1164 MVNAYAIGRDPKTWDDPLCFKPER 1235
             VN +A+ RDPK WDDPL FKPER
Sbjct: 392  FVNIWAMARDPKIWDDPLSFKPER 415


>ref|XP_004234364.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
            [Solanum lycopersicum]
          Length = 486

 Score =  358 bits (918), Expect = 3e-96
 Identities = 180/379 (47%), Positives = 261/379 (68%), Gaps = 4/379 (1%)
 Frame = +3

Query: 111  NLPPGPFSWPLLGTLGLKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAMEI 290
            NLPPGP  W LLG L      K  HV L   AQ +GPL+ +KLG + V+VAS+  +A EI
Sbjct: 30   NLPPGPRPWHLLGNL--LQIGKTPHVSLAKFAQIHGPLISVKLGTQLVVVASSPASAAEI 87

Query: 291  LKTQDHVLSGRCPPHSVQVEDYI--EASVVWA-DRNEHWKMVRKICRTELFSTKMLESQA 461
            LK+QD +LS R  P     E  +  + S+V++ D +  WK +R  CRT LFS K +ESQA
Sbjct: 88   LKSQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNQWKFLRAFCRTHLFSPKAVESQA 147

Query: 462  SIREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKDT-GMKGM 638
            ++RE++VSE++ FLR R+GE VKIS+++FG ++N LG++ F++ + D + + +T G+K +
Sbjct: 148  ALREKKVSEMMNFLRSRKGETVKISEILFGTILNTLGNLFFSKDLCDLDYETNTSGIKHV 207

Query: 639  IRELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDHSRND 818
            IR+ + L A PN+ +FYP+FD  D+QGLR+ T     R+   W+ IVKE+R +   S +D
Sbjct: 208  IRKFVELGAMPNISEFYPLFDALDLQGLRKQTEIYQNRLVNIWSEIVKEKRQAISRSSSD 267

Query: 819  FLDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVIG 998
            FLD +I   F+D Q+D L +E+ + GSD+TT+ +EWAM EL+RN     K++ EL + +G
Sbjct: 268  FLDAMIDNGFSDLQVDILLMELISAGSDTTTSTIEWAMAELLRNKGAMRKLKAELASKLG 327

Query: 999  NRQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNAY 1178
               ++ ES++  LPYL ACVKE+LR+HPP  FL+P +A ETC++M+YTIPK ++L VNA+
Sbjct: 328  ENDIITESNVSELPYLAACVKETLRIHPPTPFLIPRRAPETCKIMDYTIPKNSKLFVNAW 387

Query: 1179 AIGRDPKTWDDPLCFKPER 1235
            AIGRD  TW+D L F+PER
Sbjct: 388  AIGRDSNTWEDALSFRPER 406


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  353 bits (906), Expect = 8e-95
 Identities = 169/378 (44%), Positives = 251/378 (66%), Gaps = 3/378 (0%)
 Frame = +3

Query: 111  NLPPGPFSWPLLGTLG--LKSNEKQAHVVLTNLAQTYGPLMLLKLGVKPVIVASTHDAAM 284
            NLPP P +WP++G L   +  N       ++N+AQ YGPL+ LK G+   I AST +AAM
Sbjct: 27   NLPPSPPAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLKFGLHSSIFASTKEAAM 86

Query: 285  EILKTQDHVLSGRCPPHSVQVEDYIEASVVWADRNEHWKMVRKICRTELFSTKMLESQAS 464
            E+L+T D VLSGR P    +++ +I+ S++W+D N +WK  RKI  TE+FS KML++Q  
Sbjct: 87   EVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKILHTEIFSQKMLQAQEK 146

Query: 465  IREERVSELIAFLRRREGEVVKISDVIFGCMINVLGSVIFNQSVYDFEGKKD-TGMKGMI 641
             RE     L+ F+  + G+VV++   +FGC +NVLG V+F++ V+++  + D  GM  +I
Sbjct: 147  NRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKDVFEYSDQSDEVGMDKLI 206

Query: 642  RELMILAATPNLPDFYPIFDRFDIQGLRRTTSACWKRMSESWAGIVKERRASRDHSRNDF 821
              +++     ++  ++P+  RFD+ GL+R     +K + + W G V  RRA+R+    D 
Sbjct: 207  HGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDM 266

Query: 822  LDVLIQANFTDPQIDALFLEIFAPGSDSTTTAMEWAMTELMRNPEKRLKVQNELKTVIGN 1001
            LDVLI  +F + QI+A+F+E F PGSD+ +  +EWA+ +L++NP+K  K++ EL  V+G 
Sbjct: 267  LDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGR 326

Query: 1002 RQVVKESDLPNLPYLHACVKESLRLHPPVTFLLPHKATETCQVMNYTIPKGTQLMVNAYA 1181
               VKES    LPYL ACVKE++RL+PP++ ++PH+  ETCQVM YTIPKG  + VNA+A
Sbjct: 327  SSTVKESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHA 386

Query: 1182 IGRDPKTWDDPLCFKPER 1235
            IGRDPK W DPL F+PER
Sbjct: 387  IGRDPKDWKDPLKFQPER 404