BLASTX nr result
ID: Sinomenium21_contig00019630
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium21_contig00019630
(283 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 143 2e-32
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum] 130 1e-28
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN... 112 4e-23
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 98 1e-18
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 98 1e-18
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 98 1e-18
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 93 4e-17
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 93 4e-17
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 93 4e-17
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 93 4e-17
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 92 7e-17
emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera] 92 7e-17
ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 92 1e-16
emb|CAN70170.1| hypothetical protein VITISV_006874 [Vitis vinifera] 92 1e-16
ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 89 8e-16
ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 88 1e-15
ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 88 1e-15
ref|XP_006353351.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 88 1e-15
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 88 1e-15
ref|XP_006362519.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 87 2e-15
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 143 bits (361), Expect = 2e-32
Identities = 70/93 (75%), Positives = 81/93 (87%)
Frame = +1
Query: 4 LEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVLF 183
LE LDLQGLK++C +R RV K+WEGTVK+R+ R+EESKDMLDVLL N F+D QIN LF
Sbjct: 225 LEELDLQGLKKKCDERFIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALF 284
Query: 184 LETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
LETFGPGSETSSATIEWVIAELIK+P ++AKVR
Sbjct: 285 LETFGPGSETSSATIEWVIAELIKSPKEMAKVR 317
>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
Length = 346
Score = 130 bits (328), Expect = 1e-28
Identities = 64/94 (68%), Positives = 74/94 (78%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVL 180
I E DLQGLK+RC R V K+W+GTV++R+ R+EE KDMLDVLL N F+D QIN +
Sbjct: 84 IFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKRNEEPKDMLDVLLLNDFNDAQINAV 143
Query: 181 FLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
FLETFGPGSETSSAT EW +AELIKNP LAKVR
Sbjct: 144 FLETFGPGSETSSATSEWALAELIKNPHILAKVR 177
>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
cytochrome P-450 CYP80 [Berberis stolonifera]
Length = 487
Score = 112 bits (281), Expect = 4e-23
Identities = 53/94 (56%), Positives = 71/94 (75%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVL 180
+L DL GLKR+ ++ + + K+WEG V RR R+ E KDMLDVL+ N F++ QIN +
Sbjct: 224 VLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDMLDVLIANDFNEHQINAM 283
Query: 181 FLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
F+ETFGPGS+T+S IEW +A+LIKNPDKLAK+R
Sbjct: 284 FMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLR 317
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 97.8 bits (242), Expect = 1e-18
Identities = 48/96 (50%), Positives = 66/96 (68%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES--KDMLDVLLDNGFDDVQIN 174
IL GLD+QG+ R+ K+ ER+ WE +K+RR RS S +D L+ LL+ GF+D QIN
Sbjct: 774 ILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQIN 833
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
+ LE F G+ETSS T+EW +AELI+N D + K+R
Sbjct: 834 QVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLR 869
Score = 92.0 bits (227), Expect = 7e-17
Identities = 44/96 (45%), Positives = 68/96 (70%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES--KDMLDVLLDNGFDDVQIN 174
IL+GL+L G K++ K+ + R+ WEGT+K+RRK ++ S +D+L+ L+ F+D QIN
Sbjct: 1274 ILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQIN 1333
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
+ LE F G++TS+ TIEW I +LI+NPD + K+R
Sbjct: 1334 QVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLR 1369
Score = 91.7 bits (226), Expect = 1e-16
Identities = 46/95 (48%), Positives = 63/95 (66%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESK--DMLDVLLDNGFDDVQIN 174
IL GLD+QG++++ K+ R+ WE +K+RRK R S D LD LL+ GF+D QIN
Sbjct: 233 ILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSHGDFLDALLETGFEDDQIN 292
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
+ +E F G ETSS T+EW +AELIKN D + K+
Sbjct: 293 HVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKL 327
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 97.8 bits (242), Expect = 1e-18
Identities = 48/96 (50%), Positives = 66/96 (68%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES--KDMLDVLLDNGFDDVQIN 174
IL GLD+QG+ R+ K+ ER+ WE +K+RR RS S +D L+ LL+ GF+D QIN
Sbjct: 234 ILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQIN 293
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
+ LE F G+ETSS T+EW +AELI+N D + K+R
Sbjct: 294 QVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLR 329
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 97.8 bits (242), Expect = 1e-18
Identities = 48/96 (50%), Positives = 66/96 (68%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES--KDMLDVLLDNGFDDVQIN 174
IL GLD+QG+ R+ K+ ER+ WE +K+RR RS S +D L+ LL+ GF+D QIN
Sbjct: 234 ILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQIN 293
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
+ LE F G+ETSS T+EW +AELI+N D + K+R
Sbjct: 294 QVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLR 329
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 92.8 bits (229), Expect = 4e-17
Identities = 41/95 (43%), Positives = 68/95 (71%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEE--SKDMLDVLLDNGFDDVQIN 174
I GLDLQGL ++ K+ + R+ +WE +++RR+ S++ +D L++L+ +G+ D QIN
Sbjct: 803 IFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQIN 862
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
LF+E G++TSS+T+EW +AELIK+P+ + KV
Sbjct: 863 QLFMELLTAGADTSSSTLEWAMAELIKSPESMKKV 897
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 92.8 bits (229), Expect = 4e-17
Identities = 41/95 (43%), Positives = 68/95 (71%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEE--SKDMLDVLLDNGFDDVQIN 174
I GLDLQGL ++ K+ + R+ +WE +++RR+ S++ +D L++L+ +G+ D QIN
Sbjct: 672 IFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQIN 731
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
LF+E G++TSS+T+EW +AELIK+P+ + KV
Sbjct: 732 QLFMELLTAGADTSSSTLEWAMAELIKSPESMKKV 766
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 92.8 bits (229), Expect = 4e-17
Identities = 41/95 (43%), Positives = 68/95 (71%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEE--SKDMLDVLLDNGFDDVQIN 174
I GLDLQGL ++ K+ + R+ +WE +++RR+ S++ +D L++L+ +G+ D QIN
Sbjct: 239 IFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQIN 298
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
LF+E G++TSS+T+EW +AELIK+P+ + KV
Sbjct: 299 QLFMELLTAGADTSSSTLEWAMAELIKSPESMKKV 333
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 92.8 bits (229), Expect = 4e-17
Identities = 41/95 (43%), Positives = 68/95 (71%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEE--SKDMLDVLLDNGFDDVQIN 174
I GLDLQGL ++ K+ + R+ +WE +++RR+ S++ +D L++L+ +G+ D QIN
Sbjct: 239 IFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQIN 298
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
LF+E G++TSS+T+EW +AELIK+P+ + KV
Sbjct: 299 QLFMELLTAGADTSSSTLEWAMAELIKSPESMKKV 333
>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Vitis vinifera]
Length = 515
Score = 92.0 bits (227), Expect = 7e-17
Identities = 44/96 (45%), Positives = 68/96 (70%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES--KDMLDVLLDNGFDDVQIN 174
IL+GL+L G K++ K+ + R+ WEGT+K+RRK ++ S +D+L+ L+ F+D QIN
Sbjct: 229 ILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQIN 288
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
+ LE F G++TS+ TIEW I +LI+NPD + K+R
Sbjct: 289 QVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLR 324
>emb|CAN62646.1| hypothetical protein VITISV_013218 [Vitis vinifera]
Length = 475
Score = 92.0 bits (227), Expect = 7e-17
Identities = 44/96 (45%), Positives = 68/96 (70%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES--KDMLDVLLDNGFDDVQIN 174
IL+GL+L G K++ K+ + R+ WEGT+K+RRK ++ S +D+L+ L+ F+D QIN
Sbjct: 211 ILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQIN 270
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
+ LE F G++TS+ TIEW I +LI+NPD + K+R
Sbjct: 271 QVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLR 306
>ref|XP_002274586.2| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
[Vitis vinifera]
Length = 498
Score = 91.7 bits (226), Expect = 1e-16
Identities = 46/95 (48%), Positives = 63/95 (66%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESK--DMLDVLLDNGFDDVQIN 174
IL GLD+QG++++ K+ R+ WE +K+RRK R S D LD LL+ GF+D QIN
Sbjct: 233 ILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSHGDFLDALLETGFEDDQIN 292
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
+ +E F G ETSS T+EW +AELIKN D + K+
Sbjct: 293 HVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKL 327
>emb|CAN70170.1| hypothetical protein VITISV_006874 [Vitis vinifera]
Length = 559
Score = 91.7 bits (226), Expect = 1e-16
Identities = 46/95 (48%), Positives = 63/95 (66%), Gaps = 2/95 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESK--DMLDVLLDNGFDDVQIN 174
IL GLD+QG++++ K+ R+ WE +K+RRK R S D LD LL+ GF+D QIN
Sbjct: 233 ILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSHGDFLDALLETGFEDDQIN 292
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKV 279
+ +E F G ETSS T+EW +AELIKN D + K+
Sbjct: 293 HVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKL 327
>ref|XP_006353349.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 487
Score = 88.6 bits (218), Expect = 8e-16
Identities = 39/94 (41%), Positives = 64/94 (68%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVL 180
+ E LDLQGL+++ + R+ +W VK++R+ S S D LDV++D+GF D+QIN+L
Sbjct: 227 LFEALDLQGLRKQTEIYQSRLINIWSEIVKEKRQAISRHSSDFLDVMIDSGFSDLQINIL 286
Query: 181 FLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
E G GS+T+++TIEW + EL++N + K++
Sbjct: 287 VTELMGAGSDTTTSTIEWAMTELLRNNGAMHKLQ 320
>ref|XP_006358914.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 496
Score = 88.2 bits (217), Expect = 1e-15
Identities = 44/97 (45%), Positives = 64/97 (65%), Gaps = 3/97 (3%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEES---KDMLDVLLDNGFDDVQI 171
IL LDLQGL+++ D V + +WE +++RR +R +S +D L+ LLDNGF + I
Sbjct: 230 ILGKLDLQGLRKKSTDLVTKFCSIWEPIIEERRNSRENDSSSQQDFLETLLDNGFTNDHI 289
Query: 172 NVLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
N LF+E GS+TS+ IEW +AELIKN + + KV+
Sbjct: 290 NQLFMELLSAGSDTSTTAIEWAMAELIKNVETMKKVQ 326
>ref|XP_004234365.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Solanum lycopersicum]
Length = 486
Score = 88.2 bits (217), Expect = 1e-15
Identities = 39/94 (41%), Positives = 64/94 (68%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVL 180
+LE LDLQGL ++ K ++ +W VK++R+ + S D LDV++D+GF D+QIN+L
Sbjct: 226 LLEALDLQGLSKQTKMYQNQLINIWSQIVKEKRQAVNRRSSDFLDVMIDSGFSDLQINIL 285
Query: 181 FLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
E GS+T+++TIEW +AEL++N + K++
Sbjct: 286 LTELISAGSDTTTSTIEWAMAELLRNKGAMHKLQ 319
>ref|XP_006353351.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 491
Score = 87.8 bits (216), Expect = 1e-15
Identities = 39/94 (41%), Positives = 62/94 (65%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVL 180
+ E LDLQGL+++ + R+ +W VK++R S S D LDV++D+GF D+QIN+L
Sbjct: 231 LFEALDLQGLRKQTEIYQSRLINIWSEIVKEKRHAISRHSSDFLDVMIDSGFSDLQINIL 290
Query: 181 FLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
E G GS+T++AT EW + EL++N + K++
Sbjct: 291 LTELIGAGSDTTTATTEWAMTELLRNKGAMHKLQ 324
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 87.8 bits (216), Expect = 1e-15
Identities = 44/96 (45%), Positives = 66/96 (68%), Gaps = 2/96 (2%)
Frame = +1
Query: 1 ILEGLDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESK--DMLDVLLDNGFDDVQIN 174
+L LD QG+KR+ K+ +ER+ +W+ +K+RR+ ++++ D LDV L NGFDD QIN
Sbjct: 232 VLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQIN 291
Query: 175 VLFLETFGPGSETSSATIEWVIAELIKNPDKLAKVR 282
L LE F G++T++ T+EW +AEL+K L KVR
Sbjct: 292 WLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVR 327
>ref|XP_006362519.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 435
Score = 87.4 bits (215), Expect = 2e-15
Identities = 38/89 (42%), Positives = 64/89 (71%)
Frame = +1
Query: 13 LDLQGLKRRCKDRVERVNKLWEGTVKDRRKTRSEESKDMLDVLLDNGFDDVQINVLFLET 192
+D+QG K++ K ++ + K+WEG +K+RR++ E++ D LD++L NGF D QIN + LE
Sbjct: 167 VDIQGKKKKAKKCLDNLFKIWEGIIKERRESYVEKNGDFLDLMLANGFSDDQINYMLLEI 226
Query: 193 FGPGSETSSATIEWVIAELIKNPDKLAKV 279
F G+ T ++TIEW +AEL++N + + K+
Sbjct: 227 FPAGTGTLTSTIEWAMAELVRNKEVMKKL 255