BLASTX nr result
ID: Sinomenium21_contig00002715
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Sinomenium21_contig00002715
(683 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EXC20292.1| 26S proteasome non-ATPase regulatory subunit 11 [... 166 7e-39
ref|XP_007045996.1| 26S proteasome non-ATPase regulatory subunit... 166 9e-39
ref|XP_007222547.1| hypothetical protein PRUPE_ppa006204mg [Prun... 166 9e-39
ref|XP_004143110.1| PREDICTED: 26S proteasome non-ATPase regulat... 165 1e-38
ref|XP_002277010.1| PREDICTED: 26S proteasome non-ATPase regulat... 164 2e-38
ref|XP_002311105.1| 19S proteosome subunit 9 family protein [Pop... 164 3e-38
ref|XP_007137150.1| hypothetical protein PHAVU_009G104000g [Phas... 163 4e-38
ref|XP_002265609.1| PREDICTED: 26S proteasome non-ATPase regulat... 163 4e-38
ref|XP_003603579.1| 26S proteasome non-ATPase regulatory subunit... 163 6e-38
ref|XP_003603578.1| 26S proteasome non-ATPase regulatory subunit... 163 6e-38
gb|AFK35315.1| unknown [Lotus japonicus] 162 7e-38
gb|ACU24389.1| unknown [Glycine max] 162 7e-38
gb|ACU20905.1| unknown [Glycine max] 162 7e-38
ref|XP_006652325.1| PREDICTED: 26S proteasome non-ATPase regulat... 162 9e-38
ref|NP_001052894.1| Os04g0444600 [Oryza sativa Japonica Group] g... 162 9e-38
gb|EAY94274.1| hypothetical protein OsI_16043 [Oryza sativa Indi... 162 9e-38
ref|NP_001050534.1| Os03g0576400 [Oryza sativa Japonica Group] g... 161 2e-37
ref|XP_002319533.1| 19S proteosome subunit 9 family protein [Pop... 161 2e-37
ref|XP_004498550.1| PREDICTED: 26S proteasome non-ATPase regulat... 160 3e-37
ref|XP_003557707.1| PREDICTED: 26S proteasome non-ATPase regulat... 160 4e-37
>gb|EXC20292.1| 26S proteasome non-ATPase regulatory subunit 11 [Morus notabilis]
Length = 425
Score = 166 bits (420), Expect = 7e-39
Identities = 83/86 (96%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAI+
Sbjct: 340 LIEPFSRVEIAHIAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIY 399
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 400 PATLETISNIGKVVDSLYVRSAKIMA 425
>ref|XP_007045996.1| 26S proteasome non-ATPase regulatory subunit 11 [Theobroma cacao]
gi|508709931|gb|EOY01828.1| 26S proteasome non-ATPase
regulatory subunit 11 [Theobroma cacao]
Length = 422
Score = 166 bits (419), Expect = 9e-39
Identities = 82/86 (95%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNIGKVVDSLYVRSAKIMA 422
>ref|XP_007222547.1| hypothetical protein PRUPE_ppa006204mg [Prunus persica]
gi|462419483|gb|EMJ23746.1| hypothetical protein
PRUPE_ppa006204mg [Prunus persica]
Length = 422
Score = 166 bits (419), Expect = 9e-39
Identities = 82/86 (95%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP+DHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPIDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNIGKVVDSLYVRSAKIMA 422
>ref|XP_004143110.1| PREDICTED: 26S proteasome non-ATPase regulatory subunit 11-like
isoform 1 [Cucumis sativus]
gi|449450722|ref|XP_004143111.1| PREDICTED: 26S
proteasome non-ATPase regulatory subunit 11-like isoform
2 [Cucumis sativus] gi|449450724|ref|XP_004143112.1|
PREDICTED: 26S proteasome non-ATPase regulatory subunit
11-like isoform 3 [Cucumis sativus]
gi|449529373|ref|XP_004171674.1| PREDICTED: 26S
proteasome non-ATPase regulatory subunit 11-like isoform
1 [Cucumis sativus] gi|449529375|ref|XP_004171675.1|
PREDICTED: 26S proteasome non-ATPase regulatory subunit
11-like isoform 2 [Cucumis sativus]
gi|449529377|ref|XP_004171676.1| PREDICTED: 26S
proteasome non-ATPase regulatory subunit 11-like isoform
3 [Cucumis sativus]
Length = 422
Score = 165 bits (418), Expect = 1e-38
Identities = 82/86 (95%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP+DHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPLDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNIGKVVDSLYIRSAKIMA 422
>ref|XP_002277010.1| PREDICTED: 26S proteasome non-ATPase regulatory subunit 11 isoform
2 [Vitis vinifera] gi|225451257|ref|XP_002276988.1|
PREDICTED: 26S proteasome non-ATPase regulatory subunit
11 isoform 1 [Vitis vinifera]
gi|359487820|ref|XP_003633654.1| PREDICTED: 26S
proteasome non-ATPase regulatory subunit 11 [Vitis
vinifera] gi|147811910|emb|CAN63723.1| hypothetical
protein VITISV_021756 [Vitis vinifera]
Length = 422
Score = 164 bits (416), Expect = 2e-38
Identities = 82/86 (95%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIF+DPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFEDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNIGKVVDSLYVRSAKIMA 422
>ref|XP_002311105.1| 19S proteosome subunit 9 family protein [Populus trichocarpa]
gi|222850925|gb|EEE88472.1| 19S proteosome subunit 9
family protein [Populus trichocarpa]
Length = 422
Score = 164 bits (414), Expect = 3e-38
Identities = 79/86 (91%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+A+LIELP+DHVEKKLSQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIADLIELPIDHVEKKLSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNVGKVVDSLYVRSAKIMA 422
>ref|XP_007137150.1| hypothetical protein PHAVU_009G104000g [Phaseolus vulgaris]
gi|561010237|gb|ESW09144.1| hypothetical protein
PHAVU_009G104000g [Phaseolus vulgaris]
Length = 422
Score = 163 bits (413), Expect = 4e-38
Identities = 80/86 (93%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELPVDHVE+KLSQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPVDHVERKLSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIM+
Sbjct: 397 PATLETISNIGKVVDSLYVRSAKIMS 422
>ref|XP_002265609.1| PREDICTED: 26S proteasome non-ATPase regulatory subunit 11 [Vitis
vinifera] gi|147800876|emb|CAN73333.1| hypothetical
protein VITISV_035135 [Vitis vinifera]
Length = 422
Score = 163 bits (413), Expect = 4e-38
Identities = 81/86 (94%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFS+VEIAH+AELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIF+DPKTDAI+
Sbjct: 337 LIEPFSKVEIAHIAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFEDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNIGKVVDSLYVRSAKIMA 422
>ref|XP_003603579.1| 26S proteasome non-ATPase regulatory subunit [Medicago truncatula]
gi|355492627|gb|AES73830.1| 26S proteasome non-ATPase
regulatory subunit [Medicago truncatula]
Length = 378
Score = 163 bits (412), Expect = 6e-38
Identities = 78/86 (90%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP+DHVE+K+SQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 293 LIEPFSRVEIAHIAELIELPIDHVERKMSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 352
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 353 PATLETISNVGKVVDSLYVRSAKIMA 378
>ref|XP_003603578.1| 26S proteasome non-ATPase regulatory subunit [Medicago truncatula]
gi|355492626|gb|AES73829.1| 26S proteasome non-ATPase
regulatory subunit [Medicago truncatula]
Length = 671
Score = 163 bits (412), Expect = 6e-38
Identities = 78/86 (90%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP+DHVE+K+SQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 586 LIEPFSRVEIAHIAELIELPIDHVERKMSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 645
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 646 PATLETISNVGKVVDSLYVRSAKIMA 671
>gb|AFK35315.1| unknown [Lotus japonicus]
Length = 422
Score = 162 bits (411), Expect = 7e-38
Identities = 81/86 (94%), Positives = 85/86 (98%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP+DHVE+KLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAI
Sbjct: 337 LIEPFSRVEIAHIAELIELPIDHVERKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIC 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISNIGKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNIGKVVDSLYVRSAKIMA 422
>gb|ACU24389.1| unknown [Glycine max]
Length = 422
Score = 162 bits (411), Expect = 7e-38
Identities = 79/86 (91%), Positives = 85/86 (98%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP DHVE+KLSQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPTDHVERKLSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNVGKVVDSLYVRSAKIMA 422
>gb|ACU20905.1| unknown [Glycine max]
Length = 487
Score = 162 bits (411), Expect = 7e-38
Identities = 79/86 (91%), Positives = 85/86 (98%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP DHVE+KLSQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIAELIELPTDHVERKLSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNVGKVVDSLYVRSAKIMA 422
>ref|XP_006652325.1| PREDICTED: 26S proteasome non-ATPase regulatory subunit 11 homolog
[Oryza brachyantha]
Length = 425
Score = 162 bits (410), Expect = 9e-38
Identities = 79/86 (91%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEP+SRVEIAH+AE+IELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIF+DPKT+AIF
Sbjct: 340 LIEPYSRVEIAHIAEMIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFEDPKTEAIF 399
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 400 PATLETISNVGKVVDSLYMRSAKIMA 425
>ref|NP_001052894.1| Os04g0444600 [Oryza sativa Japonica Group]
gi|38344536|emb|CAE02442.2| OSJNBa0027P08.4 [Oryza
sativa Japonica Group] gi|38344584|emb|CAE02431.2|
OSJNBa0058G03.7 [Oryza sativa Japonica Group]
gi|113564465|dbj|BAF14808.1| Os04g0444600 [Oryza sativa
Japonica Group] gi|125590511|gb|EAZ30861.1| hypothetical
protein OsJ_14933 [Oryza sativa Japonica Group]
gi|215694007|dbj|BAG89206.1| unnamed protein product
[Oryza sativa Japonica Group]
Length = 425
Score = 162 bits (410), Expect = 9e-38
Identities = 79/86 (91%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEP+SRVEIAH+AE+IELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIF+DPKT+AIF
Sbjct: 340 LIEPYSRVEIAHIAEMIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFEDPKTEAIF 399
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 400 PATLETISNVGKVVDSLYMRSAKIMA 425
>gb|EAY94274.1| hypothetical protein OsI_16043 [Oryza sativa Indica Group]
Length = 310
Score = 162 bits (410), Expect = 9e-38
Identities = 79/86 (91%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEP+SRVEIAH+AE+IELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIF+DPKT+AIF
Sbjct: 225 LIEPYSRVEIAHIAEMIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFEDPKTEAIF 284
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 285 PATLETISNVGKVVDSLYMRSAKIMA 310
>ref|NP_001050534.1| Os03g0576400 [Oryza sativa Japonica Group]
gi|38093742|gb|AAR10858.1| putative proteosome subunit
[Oryza sativa Japonica Group]
gi|108709455|gb|ABF97250.1| PCI domain containing
protein, expressed [Oryza sativa Japonica Group]
gi|108709456|gb|ABF97251.1| PCI domain containing
protein, expressed [Oryza sativa Japonica Group]
gi|113549005|dbj|BAF12448.1| Os03g0576400 [Oryza sativa
Japonica Group] gi|125586907|gb|EAZ27571.1| hypothetical
protein OsJ_11518 [Oryza sativa Japonica Group]
Length = 310
Score = 161 bits (407), Expect = 2e-37
Identities = 78/86 (90%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEP++RVEIAH+AE+IELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIF+DPKT+AIF
Sbjct: 225 LIEPYARVEIAHIAEMIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFEDPKTEAIF 284
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 285 PATLETISNVGKVVDSLYMRSAKIMA 310
>ref|XP_002319533.1| 19S proteosome subunit 9 family protein [Populus trichocarpa]
gi|222857909|gb|EEE95456.1| 19S proteosome subunit 9
family protein [Populus trichocarpa]
Length = 422
Score = 161 bits (407), Expect = 2e-37
Identities = 79/86 (91%), Positives = 85/86 (98%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+A+LIEL VDHVEKKLSQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 337 LIEPFSRVEIAHIADLIELSVDHVEKKLSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 396
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSLY+RSAKIMA
Sbjct: 397 PATLETISNVGKVVDSLYVRSAKIMA 422
>ref|XP_004498550.1| PREDICTED: 26S proteasome non-ATPase regulatory subunit 11-like
[Cicer arietinum]
Length = 421
Score = 160 bits (406), Expect = 3e-37
Identities = 77/86 (89%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEPFSRVEIAH+AELIELP+DHVE+K+SQMILDKKFAGTLDQGAGCL+IFDDPKTDAI+
Sbjct: 336 LIEPFSRVEIAHIAELIELPMDHVERKMSQMILDKKFAGTLDQGAGCLVIFDDPKTDAIY 395
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVVDSL++RSAKIMA
Sbjct: 396 PATLETISNVGKVVDSLFVRSAKIMA 421
>ref|XP_003557707.1| PREDICTED: 26S proteasome non-ATPase regulatory subunit 11-like
[Brachypodium distachyon]
Length = 428
Score = 160 bits (405), Expect = 4e-37
Identities = 77/86 (89%), Positives = 86/86 (100%)
Frame = +2
Query: 2 LIEPFSRVEIAHVAELIELPVDHVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIF 181
LIEP+SRVEIAHVAE+IELP+DHVEKKLSQMILDKKFAGTLDQGAGCL+IF+DPKT+AIF
Sbjct: 343 LIEPYSRVEIAHVAEMIELPIDHVEKKLSQMILDKKFAGTLDQGAGCLVIFEDPKTEAIF 402
Query: 182 PATLETISNIGKVVDSLYLRSAKIMA 259
PATLETISN+GKVV+SLY+RSAKIMA
Sbjct: 403 PATLETISNVGKVVESLYMRSAKIMA 428