BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sinomenium21_contig00001317
         (1568 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   666   0.0  
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    512   e-142
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   474   e-131
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   460   e-127
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              459   e-126
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   459   e-126
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]   459   e-126
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   457   e-126
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   456   e-125
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   455   e-125
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   454   e-125
ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   448   e-123
ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   447   e-123
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              445   e-122
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   445   e-122
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   442   e-121
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   442   e-121
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   441   e-121
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy...   441   e-121
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   440   e-120

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  666 bits (1719), Expect = 0.0
 Identities = 331/456 (72%), Positives = 384/456 (84%), Gaps = 1/456 (0%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRN-HAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDR 1336
            WPL+GNL  +  N   PLHITL ++ARTHGP+M++ LGTQPTV+AST+EAAMEILKTHDR
Sbjct: 35   WPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDR 94

Query: 1335 ALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVLE 1156
              S RHIRMSFR+K   K+SLVWS+CTD WKLLRKI RTEIFSPKMLQ Q HVREQKV E
Sbjct: 95   IFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAE 154

Query: 1155 LVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRELL 976
            L++FLR +E  G+ VKISQFVFGTLLNI+GNVVFSKDVF +  +   D+ G+Q+LIRE+L
Sbjct: 155  LIDFLRSKE--GQVVKISQFVFGTLLNILGNVVFSKDVFVYGDET--DKGGIQNLIREML 210

Query: 975  MIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSESKDMLDVL 796
            MIGA PN+AEFYP LE LDLQGLK++C +R  RV K+WE TVKER+  R+ ESKDMLDVL
Sbjct: 211  MIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMKMWEGTVKERKANRNEESKDMLDVL 270

Query: 795  LDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGLHSQV 616
            L N FND QIN LFLETFGPGSETSSATIEWV+AELIK+P ++AKVR EL +VVG  S +
Sbjct: 271  LANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGT-STI 329

Query: 615  KESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYAIGRD 436
            KES LP+L YLQAC+KE MRLHPAAPFLLP RA ETC VMGYTIPK+ QVLVNAYAIGRD
Sbjct: 330  KESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRD 389

Query: 435  PNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSLVHNF 256
            P +WKDPS F PERF+ES VD++G H++FIPFGSGRR C+GMPLATRT+PLIV SLVHN+
Sbjct: 390  PKSWKDPSTFWPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNY 449

Query: 255  DWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            D+ LP G RPE+L MNEMLSL+LAIDPSL ++PK R
Sbjct: 450  DFGLPGGNRPEDLKMNEMLSLTLAIDPSLCVVPKAR 485


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  512 bits (1318), Expect = e-142
 Identities = 257/351 (73%), Positives = 294/351 (83%)
 Frame = -3

Query: 1200 MLQIQEHVREQKVLELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDE 1021
            MLQ+QEHVREQKV ELVEFL+G+E  G+ VKISQ VFGTLLNI+GNV+FSKDVF +    
Sbjct: 1    MLQVQEHVREQKVAELVEFLKGKE--GQVVKISQLVFGTLLNILGNVIFSKDVFRYV--- 55

Query: 1020 GGDEIGMQSLIRELLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKER 841
            G D   MQ+LI+E+LMIGA+PN+AEF+PI E  DLQGLK+RC  R   V K+W+ TV+ER
Sbjct: 56   GADGDKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEER 115

Query: 840  RLKRSSESKDMLDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAK 661
            ++KR+ E KDMLDVLL N FND QIN +FLETFGPGSETSSAT EW +AELIKNP  LAK
Sbjct: 116  KVKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAK 175

Query: 660  VRTELEQVVGLHSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIP 481
            VR EL+QVVG  S VKES LP L YLQAC+KETMRLHPAAPFLLPHRAVETC VMGYTIP
Sbjct: 176  VRQELDQVVG-KSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIP 234

Query: 480  KDYQVLVNAYAIGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLA 301
            KD QVLVN YAIGRDP TWK+P+ FKPERF+ES VDY G HFEFIPFG+GRR C+GMPLA
Sbjct: 235  KDTQVLVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLA 294

Query: 300  TRTVPLIVSSLVHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            TRT+PLIVS+LV   DWSLP GK P++LVMNE LSLSLA+DP+L++IPK R
Sbjct: 295  TRTIPLIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  474 bits (1219), Expect = e-131
 Identities = 236/457 (51%), Positives = 322/457 (70%), Gaps = 1/457 (0%)
 Frame = -3

Query: 1515 SWPLLGNLLTVLRNHAPLHIT-LTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            +WP++G+L  ++  ++P  +  ++++A+ +GPL+ +  G   ++ AST EAAME+L+T+D
Sbjct: 34   AWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLKFGLHSSIFASTKEAAMEVLQTND 93

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            + LSGR     FRIK    +S++WS+    WK  RKI  TEIFS KMLQ QE  RE+   
Sbjct: 94   KVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKILHTEIFSQKMLQAQEKNRERVAG 153

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
             LV F+  +  +G  V++  ++FG  LN++G+VVFSKDVF +S     DE+GM  LI  +
Sbjct: 154  NLVNFIMTK--VGDVVELRSWLFGCALNVLGHVVFSKDVFEYSDQS--DEVGMDKLIHGM 209

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSESKDMLDV 799
            LM G   ++A ++P+L   DL GLKR+  ++ + + K+WE  V  RR  R+ E KDMLDV
Sbjct: 210  LMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDMLDV 269

Query: 798  LLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGLHSQ 619
            L+ N FN+ QIN +F+ETFGPGS+T+S  IEW +A+LIKNPDKLAK+R EL++VVG  S 
Sbjct: 270  LIANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSST 329

Query: 618  VKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYAIGR 439
            VKESH   L YLQACVKETMRL+P    ++PHR +ETC+VMGYTIPK   V VNA+AIGR
Sbjct: 330  VKESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGR 389

Query: 438  DPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSLVHN 259
            DP  WKDP +F+PERF++S ++YNG  F+FIPFGSGRRIC G PLA R +PL+++SLVH 
Sbjct: 390  DPKDWKDPLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHA 449

Query: 258  FDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            F W LP G   E+L M E+ +LSL +   L +IPKVR
Sbjct: 450  FGWELPDGVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  460 bits (1184), Expect = e-127
 Identities = 241/458 (52%), Positives = 319/458 (69%), Gaps = 1/458 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            R  P++GNLL +     P H     LA+ +G L  + LG+Q  V+AS+  AA EILKTHD
Sbjct: 34   RPSPIVGNLLQL--GDKP-HAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHD 90

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            + LSGR++  SFR+KE  ++S+VWS C D WKLLRK+ RTE+F+PKM++ Q  +RE K  
Sbjct: 91   KILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAR 150

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+V+FLRG+E  G+ VKI + VFGTL+NI GN++FSKDVF      GG  + ++  + +L
Sbjct: 151  EMVKFLRGKE--GEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGS-VELKEHLWKL 207

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSESK-DMLD 802
            L +G S N A+++PI+  LDL G +R   + ++++  +W   +KERR  + SESK D +D
Sbjct: 208  LDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDFVD 267

Query: 801  VLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGLHS 622
            VLL+ G +D +IN L +E FG G+ETS++TIEW + EL K P  ++K+R EL  VVG  +
Sbjct: 268  VLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG-DN 326

Query: 621  QVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYAIG 442
             VKES LP L YLQA VKET+RLHP  P LLP RA+ETC VM YTIPK+ Q++VNA+AIG
Sbjct: 327  TVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIG 386

Query: 441  RDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSLVH 262
            RDP TW DP  FKPERF+ S VDY GN FE IPFG GRRIC G+PLA++   LIV++LV 
Sbjct: 387  RDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQ 446

Query: 261  NFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            NF+WSLP G    EL M+E   L+L  DP L I+ K R
Sbjct: 447  NFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  459 bits (1181), Expect = e-126
 Identities = 239/460 (51%), Positives = 319/460 (69%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GNLL + +N    H  L +LA+ HGPLM + LGTQ  V+AS+  AAME+LKTHDRA
Sbjct: 585  WPIIGNLLQMGKNP---HAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRA 641

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  S  +K  ++N  S+V++ +C   WK LR I R E+FS K ++ Q  +RE+KV
Sbjct: 642  LSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKV 701

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
             ELVEFL  +E  G+ VK+   VF T+ NI+ N  FS D+  F  DEG     ++ LI +
Sbjct: 702  TELVEFLATKE--GEVVKVMDLVFTTICNILSNKFFSMDLCDFE-DEGRVGGALKDLIHK 758

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSES--KDM 808
                GA+PN++++YPIL GLD+QG+ R+ K+  ER+   WE+ +KERR +RS+ S  +D 
Sbjct: 759  NAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDF 818

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L+ LL+ GF D QIN + LE F  G+ETSS T+EW MAELI+N D + K+R EL Q+VG 
Sbjct: 819  LEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVG- 877

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             S V+ESHLPRL YLQACVKE +RLHP AP LLPH A ETC+VMGYTIPKD Q+ VN +A
Sbjct: 878  ESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWA 937

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            + RDP  W DP  FKPERF++S +D+ GN FE+IPFG+GRRIC G+ L  R VPLI+++ 
Sbjct: 938  MARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATF 997

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            VH F WSLP      +L M E L ++L  +  L ++P+VR
Sbjct: 998  VHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVPRVR 1037



 Score =  451 bits (1161), Expect = e-124
 Identities = 229/460 (49%), Positives = 319/460 (69%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GNLL + +N    H+ L  LA+ HGPLM + LGTQ  V+AS+  AA+E+LKTHDR 
Sbjct: 1090 WPIIGNLLQLGKNP---HVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRT 1146

Query: 1332 LSGRHIRMSFRIKE--MNKHSLVWSN-CTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  S  +K+  +N  SL ++  CT+ WK LR I RTE+FS K ++    +RE+KV
Sbjct: 1147 LSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKV 1206

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
            +ELVEFL  +E  G+ VK+   VF T+ NI+ N  FS D+  F  +      G++  I  
Sbjct: 1207 MELVEFLATKE--GEVVKVMDLVFTTICNILSNTFFSMDLCDFERE------GLKDFIYR 1258

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRS--SESKDM 808
               +GA+PN+++FYPIL+GL+L G K++ K+ + R+   WE T+KERR +++  S  +D+
Sbjct: 1259 AAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDL 1318

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L+  L+  F D QIN + LE F  G++TS+ TIEW + +LI+NPD + K+R EL +++G 
Sbjct: 1319 LEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIG- 1377

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             S V+ESHLP L YLQACVKET+RLHP AP LLPHRA+ETC+VMGYTIPKD QV VN +A
Sbjct: 1378 ESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWA 1437

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            +GRDP  W DP  F PERF++S +++ GN FE+IPFG+GRRIC GM L  R VPL++++L
Sbjct: 1438 MGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATL 1497

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            VH FDWSLP      ++ M E L ++L  +  L ++PKVR
Sbjct: 1498 VHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVPKVR 1537



 Score =  432 bits (1111), Expect = e-118
 Identities = 227/460 (49%), Positives = 312/460 (67%), Gaps = 7/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GNLL +  N   LH+ L +LA+ HGPLM + LGTQ  V+AS+S AAME+LKTHDR 
Sbjct: 45   WPIIGNLLQMGGN---LHVKLANLAKRHGPLMSLRLGTQIMVVASSSAAAMEVLKTHDRT 101

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWSN-CTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  +  +   ++N  ++ ++  C   W+ L+ I R E+FS K ++ +  +RE+KV
Sbjct: 102  LSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMELFSGKAMESRVELRERKV 161

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIG--MQSLI 988
            +ELVEFL  +E  G+ VK+   V+ T+ NI+ N  FS D   FS  EG D  G  ++ L+
Sbjct: 162  MELVEFLEKKE--GEVVKVMDLVYTTVCNILSNKFFSID---FSDFEGRDVRGVLLKDLL 216

Query: 987  RELLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKR--SSESK 814
             E   +GA+ NI +FYPIL GLD+QG++++ K+   R+   WE+ +KERR +R   S   
Sbjct: 217  NENAELGAT-NILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSHG 275

Query: 813  DMLDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVV 634
            D LD LL+ GF D QIN + +E F  G ETSS T+EW MAELIKN D + K+  EL Q++
Sbjct: 276  DFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQII 335

Query: 633  GLHSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNA 454
            G  S V+ESHLP L YLQACVKET+RLHP  P LLPHRA ETC++MGYTIPKD  + VN 
Sbjct: 336  G-ESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNM 394

Query: 453  YAIGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVS 274
            +A+GRDP TW+DP  FKPERF++S +++ GN FE+IPFG+GRR+C GMPLA R VP+I++
Sbjct: 395  WAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILA 454

Query: 273  SLVHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPK 154
            + V  FDWS P      E+ M E   ++L  +  L ++ K
Sbjct: 455  TFVRLFDWSTPGDMDFAEIDMEERFVITLRKEQPLRLLKK 494


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 498

 Score =  459 bits (1181), Expect = e-126
 Identities = 239/460 (51%), Positives = 319/460 (69%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GNLL + +N    H  L +LA+ HGPLM + LGTQ  V+AS+  AAME+LKTHDRA
Sbjct: 45   WPIIGNLLQMGKNP---HAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRA 101

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  S  +K  ++N  S+V++ +C   WK LR I R E+FS K ++ Q  +RE+KV
Sbjct: 102  LSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKV 161

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
             ELVEFL  +E  G+ VK+   VF T+ NI+ N  FS D+  F  DEG     ++ LI +
Sbjct: 162  TELVEFLATKE--GEVVKVMDLVFTTICNILSNKFFSMDLCDFE-DEGRVGGALKDLIHK 218

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSES--KDM 808
                GA+PN++++YPIL GLD+QG+ R+ K+  ER+   WE+ +KERR +RS+ S  +D 
Sbjct: 219  NAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDF 278

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L+ LL+ GF D QIN + LE F  G+ETSS T+EW MAELI+N D + K+R EL Q+VG 
Sbjct: 279  LEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVG- 337

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             S V+ESHLPRL YLQACVKE +RLHP AP LLPH A ETC+VMGYTIPKD Q+ VN +A
Sbjct: 338  ESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWA 397

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            + RDP  W DP  FKPERF++S +D+ GN FE+IPFG+GRRIC G+ L  R VPLI+++ 
Sbjct: 398  MARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATF 457

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            VH F WSLP      +L M E L ++L  +  L ++P+VR
Sbjct: 458  VHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVPRVR 497


>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
          Length = 498

 Score =  459 bits (1181), Expect = e-126
 Identities = 239/460 (51%), Positives = 319/460 (69%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GNLL + +N    H  L +LA+ HGPLM + LGTQ  V+AS+  AAME+LKTHDRA
Sbjct: 45   WPIIGNLLQMGKNP---HAKLANLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRA 101

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  S  +K  ++N  S+V++ +C   WK LR I R E+FS K ++ Q  +RE+KV
Sbjct: 102  LSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKV 161

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
             ELVEFL  +E  G+ VK+   VF T+ NI+ N  FS D+  F  DEG     ++ LI +
Sbjct: 162  TELVEFLATKE--GEVVKVMDLVFTTICNILSNKFFSMDLCDFE-DEGRVGGALKDLIHK 218

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSES--KDM 808
                GA+PN++++YPIL GLD+QG+ R+ K+  ER+   WE+ +KERR +RS+ S  +D 
Sbjct: 219  NAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDF 278

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L+ LL+ GF D QIN + LE F  G+ETSS T+EW MAELI+N D + K+R EL Q+VG 
Sbjct: 279  LEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVG- 337

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             S V+ESHLPRL YLQACVKE +RLHP AP LLPH A ETC+VMGYTIPKD Q+ VN +A
Sbjct: 338  ESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWA 397

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            + RDP  W DP  FKPERF++S +D+ GN FE+IPFG+GRRIC G+ L  R VPLI+++ 
Sbjct: 398  MARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATF 457

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            VH F WSLP      +L M E L ++L  +  L ++P+VR
Sbjct: 458  VHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLVPRVR 497


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  457 bits (1176), Expect = e-126
 Identities = 228/460 (49%), Positives = 317/460 (68%), Gaps = 3/460 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            + WP++GNLL +     P H     LA T+G L  + LG++  V+AST  AA EILKTHD
Sbjct: 28   KPWPIVGNLLQL--GEKP-HSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHD 84

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            R LSGR++  SFR+KE  ++S+VWS C +TWK LRK+ RTE+F+ KM++ Q  VRE K +
Sbjct: 85   RVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRESKAM 144

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+VE+L+  + +G  VKI++ VFGTL+NI GN++FS+++F    DE    + M+  +  +
Sbjct: 145  EMVEYLK--KNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLG-DESSGSVEMKEHLWRM 201

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKR---SSESKDM 808
            L +G S N A+++P L   DL G  +   D ++ +  +W   +KER++ +   +S+  D 
Sbjct: 202  LELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDF 261

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            +++LLD+G +D QIN L +E FG G+ETS++TIEW ++EL KNP   A +R EL  VVG 
Sbjct: 262  VEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGK 321

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
               VKES +P + YLQA VKET+RLHPA P LLP RA+ETC+V+ YTIPK+ Q++VNA+ 
Sbjct: 322  RP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 380

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            IGRDP  W DP +F PERF+ SS+D+ GN FE IPFG+GRRIC G+PLAT+ + LIVSSL
Sbjct: 381  IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSL 440

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            V NFDW  P G  P +L+M E   L+L  +P L I+PK R
Sbjct: 441  VQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  456 bits (1173), Expect = e-125
 Identities = 226/460 (49%), Positives = 319/460 (69%), Gaps = 3/460 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            + WP++GNLL +     P H    +LA+T+G L  + LG++  V+AST  AA EILKTHD
Sbjct: 33   KPWPIVGNLLQL--GEKP-HSQFAELAKTYGDLFTLKLGSETVVVASTPLAASEILKTHD 89

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            R LSGR++  SFR+KE  ++S+VWS C +TWK LRK+ R E+F+ KM++ Q  +RE K +
Sbjct: 90   RVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEIRESKAM 149

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+VEFL+  +  G  VKI + VFGTL+NI GN++FS+++F    DE    + M+  +  +
Sbjct: 150  EMVEFLKRNQ--GSEVKIVEVVFGTLVNIFGNLIFSQNIFKLG-DESSGSVEMKEHLWRM 206

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKR---SSESKDM 808
            L +G S N A+++P L   DL G ++   D ++ +  +W   +KER++ +   +S+  D 
Sbjct: 207  LELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDF 266

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            +++LLD+G +D QIN L +E FG G+ETS++TIEW ++EL KNP+  A +R+EL  VVG 
Sbjct: 267  VEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGK 326

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
               VKES +P + YLQA VKET+RLHPA P LLP RA+ETC+V+ YTIPK+ Q++VNA+ 
Sbjct: 327  RP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 385

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            IGRDP  W DP +F PERF+ SS+D+ GN FE IPFG+GRRIC G+PLAT+ + LIV +L
Sbjct: 386  IGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVPTL 445

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            V NFDW LP G  P +L+M E   L+L  +P L I+PK R
Sbjct: 446  VQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKNR 485


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  455 bits (1171), Expect = e-125
 Identities = 227/460 (49%), Positives = 317/460 (68%), Gaps = 3/460 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            + WP++GNLL +     P H     LA T+G L  + LG++  V+AST  AA EILKTHD
Sbjct: 28   KPWPIVGNLLQL--GEKP-HSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKTHD 84

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            R LSGR++  SFR+KE  ++S+VWS C +TWK LRK+ RT++F+ KM++ Q  VRE K +
Sbjct: 85   RVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRESKAM 144

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+VE+L+  + +G  VKI++ VFGTL+NI GN++FS+++F    DE    + M+  +  +
Sbjct: 145  EMVEYLK--KNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLG-DESSGSVEMKEHLWRM 201

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKR---SSESKDM 808
            L +G S N A+++P L   DL G  +   D ++ +  +W   +KE ++ +   +S+  D 
Sbjct: 202  LELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKNDF 261

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            +++LLD+G +D QIN L +E FG G+ETS++TIEW ++EL KNP   A +R EL  VVG 
Sbjct: 262  VEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGK 321

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
               VKES +P + YLQA VKET+RLHPA P LLP RA+ETC+V+ YTIPK+ Q++VNA+ 
Sbjct: 322  RP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 380

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            IGRDP  W DP +F PERF+ SS+D+ GN FE IPFG+GRRIC G+PLAT+ + LIVSSL
Sbjct: 381  IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSL 440

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            V NFDW LP G  P +L+M E   L+L  +P L I+PK R
Sbjct: 441  VQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  454 bits (1169), Expect = e-125
 Identities = 237/458 (51%), Positives = 315/458 (68%), Gaps = 1/458 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            R  P++GNLL +     P       LA+ +G L  + LG+Q  V+AS+  AA E+LKTHD
Sbjct: 34   RPLPIVGNLLQL--GDKP-RAEFAKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLKTHD 90

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            + LSGR++  SFR+KE  ++S+VWS C D WKLLRK+ RTE+F+PKM++ Q  +RE K  
Sbjct: 91   KILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREGKAR 150

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+V+FLR +E  G+ VKI + VFGTL+NI GN++FSKDVF      GG  + ++  + +L
Sbjct: 151  EMVKFLRSKE--GEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGS-VELKEHLWKL 207

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSESK-DMLD 802
            L +G S N A+++PI+   DL G +R   D ++++  +W   +KERR  + SES  D +D
Sbjct: 208  LEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDFVD 267

Query: 801  VLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGLHS 622
            VLL+ G  D +IN L +E FG G+ETS++TIEW + EL K P  ++K+R EL  VVG  +
Sbjct: 268  VLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG-DN 326

Query: 621  QVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYAIG 442
             VKES LP L YLQA VKET+RLHP  P LLP RA+ETC VM YTIPK+ Q++VNA+AIG
Sbjct: 327  TVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIG 386

Query: 441  RDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSLVH 262
            RDP TW DP  FKPERF+ S VDY GN FE IPFG GRRIC G+PLA++ + LIV++LV 
Sbjct: 387  RDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLVQ 446

Query: 261  NFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            NF+WSLP G    EL M+E   L+L  DP L I+ K R
Sbjct: 447  NFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484


>ref|XP_002274530.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like
            [Vitis vinifera]
          Length = 515

 Score =  448 bits (1152), Expect = e-123
 Identities = 227/458 (49%), Positives = 317/458 (69%), Gaps = 5/458 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GNLL + +N    H+ L  LA+ HGPLM + LGTQ  V+AS+  AA+E+LKTHDR 
Sbjct: 45   WPIIGNLLQLGKNP---HVKLASLAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRT 101

Query: 1332 LSGRHIRMSFRIKE--MNKHSLVWSN-CTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  S  +K+  +N  SL ++  CT+ WK LR I RTE+FS K ++    +RE+KV
Sbjct: 102  LSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKV 161

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
            +ELVEFL  +E  G+ VK+   VF T+ NI+ N  FS D+  F  +      G++  I  
Sbjct: 162  MELVEFLATKE--GEVVKVMDLVFTTICNILSNTFFSMDLCDFERE------GLKDFIYR 213

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRS--SESKDM 808
               +GA+PN+++FYPIL+GL+L G K++ K+ + R+   WE T+KERR +++  S  +D+
Sbjct: 214  AAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDL 273

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L+  L+  F D QIN + LE F  G++TS+ TIEW + +LI+NPD + K+R EL +++G 
Sbjct: 274  LEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIG- 332

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             S V+ESHLP L YLQACVKET+RLHP AP LLPHRA+ETC+VMGYTIPKD QV VN +A
Sbjct: 333  ESPVRESHLPHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWA 392

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            +GRDP  W DP  F PERF++S +++ GN FE+IPFG+GRRIC GM L  R VPL++++L
Sbjct: 393  MGRDPKVWDDPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATL 452

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPK 154
            VH FDWSLP      ++ M E L ++L  +  L ++PK
Sbjct: 453  VHLFDWSLPDNMDSAQIDMEEWLVITLRKENPLRLVPK 490


>ref|XP_006358870.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2-like [Solanum tuberosum]
          Length = 495

 Score =  447 bits (1149), Expect = e-123
 Identities = 227/461 (49%), Positives = 323/461 (70%), Gaps = 6/461 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP++GN+  + +     HITLT+ A+ +GPLM + LGTQ  V+ S+S AA+EILKTHDR 
Sbjct: 41   WPVIGNIFHMGKMP---HITLTNFAKIYGPLMSLRLGTQCLVVGSSSSAAIEILKTHDRI 97

Query: 1332 LSGRHI--RMSFRIKEMNKHSLVW-SNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGRH+   M  +  +++K S+ W S C + W+ LR + +TE+FS K+L+ Q ++RE+KV
Sbjct: 98   LSGRHVPNAMPAKRSDLDKISMGWNSECHNEWRCLRTLCKTELFSGKVLESQAYLREKKV 157

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
            +ELVEFLR +E  G+ V I + VF T+LN++ NV+ SKD+     +   ++ G+++LIR 
Sbjct: 158  MELVEFLRSKE--GQVVNIGELVFATVLNMLSNVLISKDMVNLEKET--EDGGIRNLIRG 213

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKR---SSESKD 811
            L+   ++PN+++FYP+L  LDLQGL++R +  + ++   WE  ++ERR  +   SS  +D
Sbjct: 214  LVEAVSAPNLSDFYPVLGKLDLQGLRKRARYVMTKIRSNWEPILEERRKNKVSGSSSQQD 273

Query: 810  MLDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVG 631
             L+ LLDNG  +  I+ LF+E    GS+TS++TIEW MAELIKN + + KV+ ELE  + 
Sbjct: 274  FLEALLDNGLTNDCIHQLFVELVAAGSDTSTSTIEWAMAELIKNVESMKKVQEELEIELS 333

Query: 630  LHSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAY 451
                  ES L ++ Y+QAC+KET+RLHP APFLLPHRA+ETC+VM YTIPKD Q+LVN +
Sbjct: 334  ESDYPNESQLLQMSYVQACMKETLRLHPPAPFLLPHRAIETCQVMSYTIPKDAQILVNVW 393

Query: 450  AIGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSS 271
            AI RDP  W+DP  F+P+RF+ S +D+ GN FEF+PFG+GRRIC G+P+A   VPL+++S
Sbjct: 394  AITRDPLIWEDPEMFRPQRFLSSDMDFKGNDFEFLPFGAGRRICPGLPMAAIKVPLVLAS 453

Query: 270  LVHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            LVH FDW LP GKRP EL M E   ++L     L +IPK R
Sbjct: 454  LVHFFDWELPHGKRPTELDMTEKFGVTLQKKEPLLLIPKAR 494


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  445 bits (1144), Expect = e-122
 Identities = 229/460 (49%), Positives = 322/460 (70%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP+LGNLL +   + P HI+L   ++++GPL+ + LG+Q  V+ASTS AAMEILKTHDR 
Sbjct: 484  WPILGNLLHL--GNMP-HISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRV 540

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  +   K  E+N+ SL W+  C D WK LR + R E+FS K+++ Q  V E+KV
Sbjct: 541  LSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKV 600

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
            +E+V F+  +E  G+ +K+ + VF T+LN +  V+ S+D   F  D    + GM+ L+R+
Sbjct: 601  MEMVRFVSTKE--GEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDY--KDGGMKGLVRK 656

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSE--SKDM 808
            ++M  A+PN+ +FY I  GLDLQGL ++ K+ + R+  +WE  ++ERR   S +   +D 
Sbjct: 657  MMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDF 716

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L++L+ +G++D QIN LF+E    G++TSS+T+EW MAELIK+P+ + KV  EL + +  
Sbjct: 717  LNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREIS- 775

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             +  K S LP L YLQACVKET+RLHP+AP LLP RA  +C VM YTIPKD Q+ VNA+A
Sbjct: 776  DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWA 835

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            IGRDP  W+DP  FKPERF+ S+VD+ GN+ EFIPFG+GRRIC G+P+A R +PLI++SL
Sbjct: 836  IGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASL 895

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
             H FDWSLP G  P+EL MN+   ++L  +  L IIPKVR
Sbjct: 896  THFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 935


>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2-like [Vitis vinifera]
          Length = 503

 Score =  445 bits (1144), Expect = e-122
 Identities = 229/460 (49%), Positives = 322/460 (70%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP+LGNLL +   + P HI+L   ++++GPL+ + LG+Q  V+ASTS AAMEILKTHDR 
Sbjct: 51   WPILGNLLHL--GNMP-HISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRV 107

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  +   K  E+N+ SL W+  C D WK LR + R E+FS K+++ Q  V E+KV
Sbjct: 108  LSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKV 167

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
            +E+V F+  +E  G+ +K+ + VF T+LN +  V+ S+D   F  D    + GM+ L+R+
Sbjct: 168  MEMVRFVSTKE--GEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDY--KDGGMKGLVRK 223

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSE--SKDM 808
            ++M  A+PN+ +FY I  GLDLQGL ++ K+ + R+  +WE  ++ERR   S +   +D 
Sbjct: 224  MMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDF 283

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L++L+ +G++D QIN LF+E    G++TSS+T+EW MAELIK+P+ + KV  EL + +  
Sbjct: 284  LNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREIS- 342

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             +  K S LP L YLQACVKET+RLHP+AP LLP RA  +C VM YTIPKD Q+ VNA+A
Sbjct: 343  DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWA 402

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            IGRDP  W+DP  FKPERF+ S+VD+ GN+ EFIPFG+GRRIC G+P+A R +PLI++SL
Sbjct: 403  IGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASL 462

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
             H FDWSLP G  P+EL MN+   ++L  +  L IIPKVR
Sbjct: 463  THFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 502


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
            AltName: Full=Cytochrome P450 80B1
            gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  442 bits (1138), Expect = e-121
 Identities = 221/461 (47%), Positives = 313/461 (67%), Gaps = 4/461 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            + WP++GNLL +     P H    +LA+T+G +  + +GT+  V+ASTS AA EILKTHD
Sbjct: 33   KPWPIVGNLLQL--GEKP-HAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHD 89

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            R LS R++  SFR+K   ++S+VWS+CT+TWK LRK+ RTE+F+ KM++ Q HVRE+K  
Sbjct: 90   RILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCE 149

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+VE+L  ++  G+ VKI + +FGTL+NI GN++FS+++F       G     + L R +
Sbjct: 150  EMVEYLMKKQ--GEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWR-M 206

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSES----KD 811
            L +G S N A+++P+L   DL G ++   + ++ +  +W   ++ER+L +  +      D
Sbjct: 207  LELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKND 266

Query: 810  MLDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVG 631
             +DV LD+G ND QIN L +E FG G+ETS++TIEW M EL KNP   AK+R+E++ VVG
Sbjct: 267  FVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVG 326

Query: 630  LHSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAY 451
              S VKES  P L YL+A VKET+RLHP  P LLP RA+ETC ++ YTIPKD Q++VNA+
Sbjct: 327  ERS-VKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 385

Query: 450  AIGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSS 271
             IGRDP TW DP  F PERF+ SSVD+ GN F  IPFG+GRRIC G+P+A + + L+V++
Sbjct: 386  GIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVAT 445

Query: 270  LVHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
             V N DW LP G   + L++ E   L+L  +P L I+PK R
Sbjct: 446  FVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 486


>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223547255|gb|EEF48750.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  442 bits (1136), Expect = e-121
 Identities = 221/460 (48%), Positives = 321/460 (69%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            R WP+LGN+L V +N    HI++ + A+ HGPL+ + LGT+  V+AS+  AA EILKTHD
Sbjct: 42   RQWPILGNILQVGKNP---HISMANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHD 98

Query: 1338 RALSGRHIRMSFRIKE--MNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQ 1168
            R LSGR+I  +   ++  +++ +LVW+ +C+D WK LR + R+E+FS K ++ Q  +RE+
Sbjct: 99   RLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREK 158

Query: 1167 KVLELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLI 988
            K+ E+++FL  ++  G+ V I + VF T  N I N++FSKD+  F       E+  ++LI
Sbjct: 159  KLTEMLDFLTSKQ--GQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAGEL--KTLI 214

Query: 987  RELLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSESK-- 814
              L+ +   PNIA+FYP+L  LD QG+KR+ K+ +ER+  +W+  +KERR +   +++  
Sbjct: 215  STLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHVKDARKT 274

Query: 813  DMLDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVV 634
            D LDV L NGF+D QIN L LE F  G++T++ T+EW MAEL+K    L KVR ELE  +
Sbjct: 275  DFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEI 334

Query: 633  GLHSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNA 454
            G    ++ESH+P+L YL ACVKET+RLHP  PFL+P RA E C VM YTIPK  Q++VN 
Sbjct: 335  G-KDMIRESHIPQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNV 393

Query: 453  YAIGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVS 274
            +AIGRDP+ W+DP  ++PERF++S++D+ G++FEF+PFGSGRRIC G+P+ TR +PLI++
Sbjct: 394  WAIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILA 453

Query: 273  SLVHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPK 154
            SLVH FDWSL  G  P  L MN+  S++L  +  L ++PK
Sbjct: 454  SLVHCFDWSLQNGDDPAMLDMNDKFSITLEKEQHLLVVPK 493


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
            AltName: Full=Cytochrome P450 80B2
            gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  441 bits (1135), Expect = e-121
 Identities = 222/461 (48%), Positives = 313/461 (67%), Gaps = 4/461 (0%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            + WP++GNLL +     P H    +LA+T+G +  + +GT+  V+ASTS AA EILKTHD
Sbjct: 34   KPWPIVGNLLQL--GEKP-HAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHD 90

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            R LS R++  SFR+K   ++S+VWS+CT+TWK LRK+ RTE+F+ KM++ Q HVRE+K  
Sbjct: 91   RILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCE 150

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            E+VE+L  ++  G+ VKI + +FGTL+NI GN++FS+++F       G     + L R +
Sbjct: 151  EMVEYLMKKQ--GEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWR-M 207

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSES----KD 811
            L +G S N A+++P+L   DL G ++   + ++ +  +W   ++ER+L +  +      D
Sbjct: 208  LELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKND 267

Query: 810  MLDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVG 631
             +DV LD+G ND QIN L +E FG G+ETS++TIEW M EL KNP   AK+R+EL+ VVG
Sbjct: 268  FVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVG 327

Query: 630  LHSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAY 451
              S VKES  P L YL+A VKET+RLHP  P LLP RA+ETC ++ YTIPKD Q++VNA+
Sbjct: 328  ERS-VKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 386

Query: 450  AIGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSS 271
             IGRDP TW DP  F PERF+ SSVD+ GN F  IPFG+GRRIC G+P+A + + L+V++
Sbjct: 387  GIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVAT 446

Query: 270  LVHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
             V N DW LP G   + L++ E   L+L  +P L I+PK R
Sbjct: 447  FVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487


>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
            [Vitis vinifera]
          Length = 503

 Score =  441 bits (1134), Expect = e-121
 Identities = 228/460 (49%), Positives = 320/460 (69%), Gaps = 5/460 (1%)
 Frame = -3

Query: 1512 WPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHDRA 1333
            WP+LGNLL +   + P HI+L   ++++GPL+ + LG+Q  V+ASTS AAMEILKTHDR 
Sbjct: 51   WPILGNLLHL--GNMP-HISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRV 107

Query: 1332 LSGRHIRMSFRIK--EMNKHSLVWS-NCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKV 1162
            LSGR++  +   K  E+N  SL W+  C   WK LR + R E+FS K+++ Q  V E+KV
Sbjct: 108  LSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKV 167

Query: 1161 LELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIRE 982
            +E+V F+  +E  G+ +K+ + VF T+LN +  V+ S+D   F  D    + GM+ L+R+
Sbjct: 168  MEMVRFVSTKE--GEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDN--KDGGMKGLVRK 223

Query: 981  LLMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSE--SKDM 808
            ++M  A+PN+ +FY I  GLDLQGL ++ K+ + R+  +WE  ++ERR   S +   +D 
Sbjct: 224  MVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQDF 283

Query: 807  LDVLLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGL 628
            L++L+ +G++D QIN LF+E    G++TSS+T+EW MAELIK+P+ + KV  EL + +  
Sbjct: 284  LNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREIS- 342

Query: 627  HSQVKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYA 448
             +  K S LP L YLQACVKET+RLHP+AP LLP RA  +C VM YTIPKD Q+ VNA+A
Sbjct: 343  DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWA 402

Query: 447  IGRDPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSL 268
            IGRDP  W+DP  FKPERF+ S+VD+ GN+ EFIPFG+GRRIC G+P+A R +PLI++SL
Sbjct: 403  IGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASL 462

Query: 267  VHNFDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
             H FDWSLP G  P+EL MN+   ++L  +  L IIPKVR
Sbjct: 463  THFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVR 502


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
            glaucum]
          Length = 491

 Score =  440 bits (1131), Expect = e-120
 Identities = 229/457 (50%), Positives = 306/457 (66%)
 Frame = -3

Query: 1518 RSWPLLGNLLTVLRNHAPLHITLTDLARTHGPLMLVTLGTQPTVLASTSEAAMEILKTHD 1339
            R  P++GNLL +     P H     LA  +G L  + LG+Q  V+AS+  AA EILKT D
Sbjct: 35   RPSPIVGNLLQL--GEKP-HAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAEILKTRD 91

Query: 1338 RALSGRHIRMSFRIKEMNKHSLVWSNCTDTWKLLRKIARTEIFSPKMLQIQEHVREQKVL 1159
            + LSGR++  SFR+ E   +S+VWS C + WKLLRK+ RTE+FSPKM++ Q ++RE K L
Sbjct: 92   KILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYIREAKAL 151

Query: 1158 ELVEFLRGREYLGKAVKISQFVFGTLLNIIGNVVFSKDVFGFSSDEGGDEIGMQSLIREL 979
            ++V FLR +E   + VKI + VF TL+NI GN++FSKDVF      GG    ++  + +L
Sbjct: 152  DMVRFLRKKE--NQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSA-ELKEHLWKL 208

Query: 978  LMIGASPNIAEFYPILEGLDLQGLKRRCKDRVERVNKLWEETVKERRLKRSSESKDMLDV 799
            L +G S N A+++PI+   DL G +R     ++++  +W   +KERR        D  +V
Sbjct: 209  LDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGHRENDFANV 268

Query: 798  LLDNGFNDVQINVLFLETFGPGSETSSATIEWVMAELIKNPDKLAKVRTELEQVVGLHSQ 619
            LL+ G +D +IN L +E FG G+ET+++TIEW + EL  N   ++K+R EL  VVG H  
Sbjct: 269  LLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVG-HKT 327

Query: 618  VKESHLPRLHYLQACVKETMRLHPAAPFLLPHRAVETCRVMGYTIPKDYQVLVNAYAIGR 439
            VKES++P L YLQA VKET+RLHP  P LLP RA+ETC+VM YTIPK+ Q++VNA+AIGR
Sbjct: 328  VKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWAIGR 387

Query: 438  DPNTWKDPSRFKPERFMESSVDYNGNHFEFIPFGSGRRICIGMPLATRTVPLIVSSLVHN 259
            DP TW DP  FKPERFM S+VDY GN FE IPFG GRRIC G+PLA++ + LIV++LV N
Sbjct: 388  DPKTWDDPLTFKPERFMNSTVDYKGNDFELIPFGGGRRICPGLPLASQFLSLIVATLVQN 447

Query: 258  FDWSLPAGKRPEELVMNEMLSLSLAIDPSLSIIPKVR 148
            FDWS P G  P E+ M+E   L L  DP L I+ K R
Sbjct: 448  FDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLIVLKAR 484