BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sinomenium21_contig00000230
         (292 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284928.1| PREDICTED: polyneuridine-aldehyde esterase [...   120   3e-25
ref|XP_002284944.1| PREDICTED: polyneuridine-aldehyde esterase [...   118   1e-24
emb|CAN67986.1| hypothetical protein VITISV_010770 [Vitis vinifera]   118   1e-24
ref|XP_002284950.2| PREDICTED: polyneuridine-aldehyde esterase-l...   117   1e-24
emb|CBI32472.3| unnamed protein product [Vitis vinifera]              117   1e-24
ref|XP_002284907.2| PREDICTED: polyneuridine-aldehyde esterase-l...   117   2e-24
ref|XP_006480147.1| PREDICTED: methylesterase 10-like [Citrus si...   113   3e-23
ref|XP_006423079.1| hypothetical protein CICLE_v10028977mg [Citr...   111   9e-23
ref|XP_007199380.1| hypothetical protein PRUPE_ppa025654mg [Prun...   110   3e-22
ref|XP_007200274.1| hypothetical protein PRUPE_ppa014939mg [Prun...   106   3e-21
ref|XP_002313085.2| hypothetical protein POPTR_0009s11070g [Popu...    99   6e-19
ref|XP_006379213.1| hypothetical protein POPTR_0009s11050g [Popu...    96   7e-18
ref|XP_002313084.2| hypothetical protein POPTR_0009s11040g [Popu...    96   7e-18
ref|XP_007042333.1| Methyl esterase 10, putative isoform 1 [Theo...    95   1e-17
ref|XP_006361568.1| PREDICTED: polyneuridine-aldehyde esterase-l...    94   3e-17
ref|XP_004243298.1| PREDICTED: polyneuridine-aldehyde esterase-l...    94   3e-17
ref|XP_004243297.1| PREDICTED: polyneuridine-aldehyde esterase-l...    94   3e-17
gb|ADK38539.1| methylketone synthase Id [Solanum lycopersicum]         94   3e-17
ref|XP_006366937.1| PREDICTED: polyneuridine-aldehyde esterase-l...    93   3e-17
ref|XP_002310759.2| hypothetical protein POPTR_0007s11740g [Popu...    93   4e-17

>ref|XP_002284928.1| PREDICTED: polyneuridine-aldehyde esterase [Vitis vinifera]
           gi|297741350|emb|CBI32481.3| unnamed protein product
           [Vitis vinifera]
          Length = 265

 Score =  120 bits (300), Expect = 3e-25
 Identities = 57/98 (58%), Positives = 73/98 (74%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           K++ AVFVTA MPGP LN ST+ QES RR+GP +D Q+ Y +GP  PPT + FGP+   +
Sbjct: 104 KVSVAVFVTASMPGPTLNISTLNQESLRRQGPLLDSQFTYDNGPNNPPTTFTFGPLFSSL 163

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            +Y LSP EDL LGTML+RP  LFS ED+SK+++LS K
Sbjct: 164 NVYQLSPTEDLALGTMLMRPLRLFSEEDMSKDLMLSKK 201


>ref|XP_002284944.1| PREDICTED: polyneuridine-aldehyde esterase [Vitis vinifera]
           gi|297741344|emb|CBI32475.3| unnamed protein product
           [Vitis vinifera]
          Length = 265

 Score =  118 bits (295), Expect = 1e-24
 Identities = 56/98 (57%), Positives = 72/98 (73%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           K++ AVFVTA MPGP LN ST+ QES RR+GP +D Q+ Y +GP  PPT + FGP+   +
Sbjct: 104 KVSVAVFVTASMPGPTLNISTLNQESLRRQGPLLDSQFTYDNGPNNPPTTFTFGPLFSSL 163

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            +Y LSP EDL LGTML+RP  LFS ED+S +++LS K
Sbjct: 164 NVYQLSPTEDLALGTMLMRPLRLFSEEDMSNDLMLSKK 201


>emb|CAN67986.1| hypothetical protein VITISV_010770 [Vitis vinifera]
          Length = 674

 Score =  118 bits (295), Expect = 1e-24
 Identities = 56/98 (57%), Positives = 72/98 (73%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           K++ AVFVTA MPGP LN ST+ QES RR+GP +D Q+ Y +GP  PPT + FGP+   +
Sbjct: 513 KVSVAVFVTASMPGPTLNISTLNQESLRRQGPLLDSQFTYDNGPNNPPTTFTFGPLFSSL 572

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            +Y LSP EDL LGTML+RP  LFS ED+S +++LS K
Sbjct: 573 NVYQLSPTEDLALGTMLMRPLRLFSEEDMSNDLMLSKK 610


>ref|XP_002284950.2| PREDICTED: polyneuridine-aldehyde esterase-like [Vitis vinifera]
          Length = 288

 Score =  117 bits (294), Expect = 1e-24
 Identities = 56/98 (57%), Positives = 72/98 (73%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           K++ AVFVTA MPGP LN ST+ QE++RR+GP +D Q+ Y +GP  PPT + FGP+ + +
Sbjct: 127 KVSVAVFVTASMPGPTLNISTLNQETSRRQGPLLDSQFTYDNGPNNPPTTFTFGPLFLSL 186

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            LY LSP EDL LGTML+RP  LFS ED S E++L  K
Sbjct: 187 NLYQLSPTEDLALGTMLMRPVRLFSEEDTSNELMLWKK 224


>emb|CBI32472.3| unnamed protein product [Vitis vinifera]
          Length = 314

 Score =  117 bits (294), Expect = 1e-24
 Identities = 56/98 (57%), Positives = 72/98 (73%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           K++ AVFVTA MPGP LN ST+ QE++RR+GP +D Q+ Y +GP  PPT + FGP+ + +
Sbjct: 153 KVSVAVFVTASMPGPTLNISTLNQETSRRQGPLLDSQFTYDNGPNNPPTTFTFGPLFLSL 212

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            LY LSP EDL LGTML+RP  LFS ED S E++L  K
Sbjct: 213 NLYQLSPTEDLALGTMLMRPVRLFSEEDTSNELMLWKK 250


>ref|XP_002284907.2| PREDICTED: polyneuridine-aldehyde esterase-like [Vitis vinifera]
           gi|297741357|emb|CBI32488.3| unnamed protein product
           [Vitis vinifera]
          Length = 265

 Score =  117 bits (293), Expect = 2e-24
 Identities = 55/98 (56%), Positives = 73/98 (74%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           K++ AVFVTA MPGP LN ST+ QES RR+GP +D Q+ Y +GP  PPT ++FGP+ + +
Sbjct: 104 KVSVAVFVTASMPGPTLNISTLNQESLRRQGPLLDSQFTYDNGPNNPPTTFSFGPLFLSL 163

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            +Y LSP EDL LGT+L+RP  LF  ED+S E++LS K
Sbjct: 164 NVYQLSPTEDLALGTVLMRPVRLFIEEDMSNELMLSKK 201


>ref|XP_006480147.1| PREDICTED: methylesterase 10-like [Citrus sinensis]
          Length = 289

 Score =  113 bits (282), Expect = 3e-23
 Identities = 56/96 (58%), Positives = 70/96 (72%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVFVTALMPGP LN ST+ QES  R+GP +D +Y Y DGP  PPT + FGP+ ++ 
Sbjct: 127 KISVAVFVTALMPGPDLNISTLNQESFSRQGPLLDCKYAYDDGPDSPPTTFIFGPLYLKS 186

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            +Y LSP ED  L TML+RP  LFS ED+SKE+ L+
Sbjct: 187 KVYQLSPVEDWALATMLMRPLGLFSEEDMSKELKLT 222


>ref|XP_006423079.1| hypothetical protein CICLE_v10028977mg [Citrus clementina]
           gi|557525013|gb|ESR36319.1| hypothetical protein
           CICLE_v10028977mg [Citrus clementina]
          Length = 289

 Score =  111 bits (278), Expect = 9e-23
 Identities = 55/96 (57%), Positives = 70/96 (72%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVFV+ALMPGP LN ST+ QES  R+GP +D +Y Y DGP  PPT + FGP+ ++ 
Sbjct: 127 KISVAVFVSALMPGPDLNISTLNQESFSRQGPLLDCKYAYDDGPDSPPTTFIFGPLYLKS 186

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            +Y LSP ED  L TML+RP  LFS ED+SKE+ L+
Sbjct: 187 KVYQLSPVEDWALATMLMRPLGLFSEEDMSKELKLT 222


>ref|XP_007199380.1| hypothetical protein PRUPE_ppa025654mg [Prunus persica]
           gi|462394780|gb|EMJ00579.1| hypothetical protein
           PRUPE_ppa025654mg [Prunus persica]
          Length = 268

 Score =  110 bits (274), Expect = 3e-22
 Identities = 55/96 (57%), Positives = 68/96 (70%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVFVTALMPGP LN ST+ QES RR+G  +D +Y Y  GP  PP    FGP+ +  
Sbjct: 106 KISLAVFVTALMPGPTLNISTLNQESFRRQGSLLDSKYTYDQGPNNPPITLTFGPLFLAT 165

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            +Y LSP ED+ LGT+L+RP  LFS EDLSKE+ L+
Sbjct: 166 NMYQLSPTEDVALGTILMRPQRLFSEEDLSKELKLT 201


>ref|XP_007200274.1| hypothetical protein PRUPE_ppa014939mg [Prunus persica]
           gi|462395674|gb|EMJ01473.1| hypothetical protein
           PRUPE_ppa014939mg [Prunus persica]
          Length = 269

 Score =  106 bits (265), Expect = 3e-21
 Identities = 54/96 (56%), Positives = 66/96 (68%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+  VF+TALMPGP LN ST+ QES RR+   +D +Y Y  GP  PP    FGP+ +  
Sbjct: 107 KISLVVFITALMPGPTLNISTLNQESFRRQRSLLDSKYTYDQGPNYPPITLTFGPLFLAT 166

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            +Y LSP EDL LGTML+RP  LFS EDLSKE+ L+
Sbjct: 167 NVYQLSPTEDLALGTMLMRPQRLFSEEDLSKELKLT 202


>ref|XP_002313085.2| hypothetical protein POPTR_0009s11070g [Populus trichocarpa]
           gi|550331488|gb|EEE87040.2| hypothetical protein
           POPTR_0009s11070g [Populus trichocarpa]
          Length = 289

 Score = 99.0 bits (245), Expect = 6e-19
 Identities = 49/96 (51%), Positives = 69/96 (71%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF+TA+MPGP+LN ST+ QE  RR    +D +Y +G+GP  PPT+  FGP  + +
Sbjct: 127 KISVAVFLTAVMPGPSLNISTLSQELARRLTDMLDTRYTFGNGPNNPPTSLTFGPKYLLL 186

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            LY LSP ED TL T L+R T LF+ ++LS+++VL+
Sbjct: 187 RLYQLSPIEDWTLATTLMRETRLFTDQELSRDLVLT 222


>ref|XP_006379213.1| hypothetical protein POPTR_0009s11050g [Populus trichocarpa]
           gi|550331486|gb|ERP57010.1| hypothetical protein
           POPTR_0009s11050g [Populus trichocarpa]
          Length = 289

 Score = 95.5 bits (236), Expect = 7e-18
 Identities = 47/96 (48%), Positives = 68/96 (70%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF+TA MPGP+LN ST+ QE  RR    +D ++ + +GP  PPT++ FGP  + +
Sbjct: 127 KISVAVFLTAFMPGPSLNISTLNQERVRRLTDMLDTRFTFDNGPNNPPTSFIFGPKHLLL 186

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            LY LSP ED TL T L+R T LF+ ++LS+++VL+
Sbjct: 187 RLYQLSPIEDWTLATTLMRETRLFTDQELSRDLVLT 222


>ref|XP_002313084.2| hypothetical protein POPTR_0009s11040g [Populus trichocarpa]
           gi|550331485|gb|EEE87039.2| hypothetical protein
           POPTR_0009s11040g [Populus trichocarpa]
          Length = 289

 Score = 95.5 bits (236), Expect = 7e-18
 Identities = 47/96 (48%), Positives = 69/96 (71%), Gaps = 1/96 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF+TA+MPGP+LN ST+ QE  RR+   +D ++ + +GP  PPT+  FGP  + +
Sbjct: 127 KISVAVFLTAVMPGPSLNISTLNQERVRRQTDMLDTRFTFDNGPNNPPTSLIFGPKFLLL 186

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLS 8
            LY LSP ED TL T L+R T LF+ ++LS+++VL+
Sbjct: 187 RLYQLSPIEDWTLATTLMRETRLFTDQELSRDLVLT 222


>ref|XP_007042333.1| Methyl esterase 10, putative isoform 1 [Theobroma cacao]
           gi|508706268|gb|EOX98164.1| Methyl esterase 10, putative
           isoform 1 [Theobroma cacao]
          Length = 300

 Score = 94.7 bits (234), Expect = 1e-17
 Identities = 47/98 (47%), Positives = 65/98 (66%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF+TALMPGP LN ST+ Q + + +  ++D  Y Y +GP   PT + FGP+ +  
Sbjct: 138 KISVAVFLTALMPGPTLNISTLSQRAFKEQDSQMDSLYTYDNGPNNAPTTFIFGPLYLSS 197

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
            +Y LSP ED  L  +L+RP  L+S ED+S E+VLS K
Sbjct: 198 QVYQLSPREDWALANVLMRPIPLYSMEDMSTELVLSKK 235


>ref|XP_006361568.1| PREDICTED: polyneuridine-aldehyde esterase-like [Solanum tuberosum]
          Length = 266

 Score = 93.6 bits (231), Expect = 3e-17
 Identities = 50/98 (51%), Positives = 66/98 (67%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF++ +MPGP ++AS V  E+      E+D +  Y +GP+ PPT +  GP  +  
Sbjct: 104 KISVAVFLSGVMPGPNISASIVYTETINAIIREVDNRVTYHNGPENPPTTFILGPKFLET 163

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
             YHLSP EDL L T LVRP  L+S+ED+SKEIVLSSK
Sbjct: 164 NAYHLSPIEDLALATTLVRPFYLYSAEDVSKEIVLSSK 201


>ref|XP_004243298.1| PREDICTED: polyneuridine-aldehyde esterase-like isoform 2 [Solanum
           lycopersicum]
          Length = 325

 Score = 93.6 bits (231), Expect = 3e-17
 Identities = 50/98 (51%), Positives = 66/98 (67%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF++ +MPGP ++AS V  E+      E+D +  Y +G + PPT +N GP  +  
Sbjct: 165 KISVAVFLSGVMPGPNISASIVYTEAINAIIRELDNRVTYHNGSENPPTTFNLGPKFLET 224

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
             YHLSP EDL L T LVRP  L+S+ED+SKEIVLSSK
Sbjct: 225 NAYHLSPIEDLALATTLVRPFYLYSAEDVSKEIVLSSK 262


>ref|XP_004243297.1| PREDICTED: polyneuridine-aldehyde esterase-like isoform 1 [Solanum
           lycopersicum]
          Length = 327

 Score = 93.6 bits (231), Expect = 3e-17
 Identities = 50/98 (51%), Positives = 66/98 (67%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF++ +MPGP ++AS V  E+      E+D +  Y +G + PPT +N GP  +  
Sbjct: 165 KISVAVFLSGVMPGPNISASIVYTEAINAIIRELDNRVTYHNGSENPPTTFNLGPKFLET 224

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
             YHLSP EDL L T LVRP  L+S+ED+SKEIVLSSK
Sbjct: 225 NAYHLSPIEDLALATTLVRPFYLYSAEDVSKEIVLSSK 262


>gb|ADK38539.1| methylketone synthase Id [Solanum lycopersicum]
          Length = 264

 Score = 93.6 bits (231), Expect = 3e-17
 Identities = 50/98 (51%), Positives = 66/98 (67%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF++ +MPGP ++AS V  E+      E+D +  Y +G + PPT +N GP  +  
Sbjct: 102 KISVAVFLSGVMPGPNISASIVYTEAINAIIRELDNRVTYHNGSENPPTTFNLGPKFLET 161

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
             YHLSP EDL L T LVRP  L+S+ED+SKEIVLSSK
Sbjct: 162 NAYHLSPIEDLALATTLVRPFYLYSAEDVSKEIVLSSK 199


>ref|XP_006366937.1| PREDICTED: polyneuridine-aldehyde esterase-like [Solanum tuberosum]
          Length = 266

 Score = 93.2 bits (230), Expect = 3e-17
 Identities = 50/98 (51%), Positives = 66/98 (67%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+ AVF++ +MPGP ++AS V  E+      E+D +  Y +GP+ PPT +  GP  +  
Sbjct: 104 KISVAVFLSGVMPGPNISASIVYTETINAIIRELDNRVTYHNGPENPPTTFILGPKFLET 163

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
             YHLSP EDL L T LVRP  L+S+ED+SKEIVLSSK
Sbjct: 164 NAYHLSPIEDLALATTLVRPFYLYSAEDVSKEIVLSSK 201


>ref|XP_002310759.2| hypothetical protein POPTR_0007s11740g [Populus trichocarpa]
           gi|550334687|gb|EEE91209.2| hypothetical protein
           POPTR_0007s11740g [Populus trichocarpa]
          Length = 333

 Score = 92.8 bits (229), Expect = 4e-17
 Identities = 45/98 (45%), Positives = 66/98 (67%), Gaps = 1/98 (1%)
 Frame = -1

Query: 292 KIAAAVFVTALMPGPALNASTVRQESTRRRGPEIDFQYIYGDGPQKPPTAYNFGPITMRI 113
           KI+AAVF  A+MPGP L+   + ++S++     +D QY++G+GP  PPTA   GP  M  
Sbjct: 174 KISAAVFAAAVMPGPDLSFKAIAEKSSQTSVSYMDTQYVFGNGPGNPPTAVVLGPNYMAS 233

Query: 112 -LYHLSPPEDLTLGTMLVRPTSLFSSEDLSKEIVLSSK 2
             YHLSPPEDLTL T+LVRP  ++SS +  K ++++ +
Sbjct: 234 RFYHLSPPEDLTLATLLVRPFPIYSSLETEKAVIVTKE 271