BLASTX nr result
ID: Scutellaria24_contig00014247
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Scutellaria24_contig00014247
(1174 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|2... 222 1e-55
sp|Q70PR7.2|VINSY_RAUSE RecName: Full=Vinorine synthase gi|60594... 215 2e-53
ref|XP_002514981.1| Salutaridinol 7-O-acetyltransferase, putativ... 214 4e-53
ref|XP_002875299.1| transferase family protein [Arabidopsis lyra... 212 1e-52
ref|XP_003538872.1| PREDICTED: vinorine synthase-like [Glycine max] 207 5e-51
>ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|222864679|gb|EEF01810.1|
predicted protein [Populus trichocarpa]
Length = 430
Score = 222 bits (566), Expect = 1e-55
Identities = 127/335 (37%), Positives = 204/335 (60%), Gaps = 15/335 (4%)
Frame = +2
Query: 5 FVECNDRGVKFVEAQVHARLSQVIDEPQMEELKQLVPMDSFEHSESAILAVKTNVFDCGG 184
FVECND G+ F +A+V RL QV++ P+ EL +L+P + + +E L ++ N+F+CGG
Sbjct: 86 FVECNDEGIPFFQAEVKCRLPQVVENPEPSELNKLIPF-ALDDAEELPLGIQYNIFECGG 144
Query: 185 MAVGVCLSHKVADFASSVVFMDAWASMCRGESS--TQPLFDLALHFPPRDSTLSNFLP-- 352
+ +G+C+SHKV D +S F+ WA+ RGE+ ++P F A FPP + +S F P
Sbjct: 145 IVIGLCISHKVGDASSLFTFIKYWAATARGEADHISRPEFISATLFPPIN--ISGFKPAT 202
Query: 353 --DNENNKTQRFVFDKEKLEMLQRRTS--SKEVEN---PTKVEALTVFLSQQFIEATKTI 511
E+ T+RFVF +E+L+ + S S +EN P++VEAL+VF+ Q+F A+K
Sbjct: 203 GITKEDVVTKRFVFRSSSIELLKEKCSPASGSLENQRPPSRVEALSVFIWQRFTAASKVE 262
Query: 512 SSTQNKFLIHHAMNLRSRTIPPIPDHTIGN-CITSFH--AIITSDDSSDLMISKLRAAKN 682
S + + + HA+NLRSR PP+P+++ GN C +F +I T +++ +L S++R +
Sbjct: 263 SRPERIYSMVHAVNLRSRMEPPLPEYSFGNYCRIAFTIPSIDTGEENYNLA-SQIRDSIG 321
Query: 683 SVDNNFIKEVINTKEYLGTIDKLTELVMSG-AESLIFTSLWRYPVYDVDFGWGKPIWVSD 859
VD ++K++ E+LG + + + G L FTSL R+P+Y+ DFGW KPIWV
Sbjct: 322 KVDKEYVKKLQKGNEHLGFMKEQAARFLRGEVVPLNFTSLCRFPLYEADFGWAKPIWVGS 381
Query: 860 STVASKNTITFTTTSSKDGIEAWINMPPDFLKMIE 964
++ KN + F T+S DGIEA +++ + + E
Sbjct: 382 PSLTFKNLVVFMDTASGDGIEALVHLKEEDMAKFE 416
>sp|Q70PR7.2|VINSY_RAUSE RecName: Full=Vinorine synthase gi|60594431|pdb|2BGH|A Chain A,
Crystal Structure Of Vinorine Synthase
gi|60594432|pdb|2BGH|B Chain B, Crystal Structure Of
Vinorine Synthase gi|57635335|emb|CAD89104.2| vinorine
synthase [Rauvolfia serpentina]
Length = 421
Score = 215 bits (547), Expect = 2e-53
Identities = 129/339 (38%), Positives = 192/339 (56%), Gaps = 11/339 (3%)
Frame = +2
Query: 2 SFVECNDRGVKFVEAQVHARLSQVIDEP-QMEELKQLVPMDSF-----EHSESAILAVKT 163
S V+CND GV FVEA+V A+LSQ I ++E+L Q +P ++ E +E LAVK
Sbjct: 85 SSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLPSAAYPGGKIEVNEDVPLAVKI 144
Query: 164 NVFDCGGMAVGVCLSHKVADFASSVVFMDAWASMCRGESS-TQPLFDLAL-HFPPRDSTL 337
+ F+CGG A+GV LSHK+AD S F++AW + CRGE+ P FDLA HFPP D+T
Sbjct: 145 SFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGETEIVLPNFDLAARHFPPVDNTP 204
Query: 338 SNFLPDNENNKTQRFVFDKEKLEMLQRRTSS-KEVENPTKVEALTVFLSQQFIEATKTIS 514
S L +EN +RFVFDKEK+ L+ + SS E +N ++V+ + ++ + I+ T+
Sbjct: 205 SPELVPDENVVMKRFVFDKEKIGALRAQASSASEEKNFSRVQLVVAYIWKHVIDVTRAKY 264
Query: 515 STQNKFLIHHAMNLRSRTIPPIPDHTIGNCITSFHAIITSDDSSDL--MISKLRAAKNSV 688
+NKF++ A+NLRSR PP+P + +GN T A + ++ D +I LR +
Sbjct: 265 GAKNKFVVVQAVNLRSRMNPPLPHYAMGNIATLLFAAVDAEWDKDFPDLIGPLRTSLEKT 324
Query: 689 DNNFIKEVINTKEYLGTIDKLTELVMSGAESLIFTSLWRYPVYDVDFGWGKPIWVSDSTV 868
+++ E++ L ++ E L FTS R YD+DFGWGKP+ +T
Sbjct: 325 EDDHNHELLKGMTCLYELEP--------QELLSFTSWCRLGFYDLDFGWGKPLSACTTTF 376
Query: 869 ASKNTITFTTTSSKDGIEAWINMPPDFLKMIEINYNKLL 985
+N T S DG+EAW+ M D + M+ + L+
Sbjct: 377 PKRNAALLMDTRSGDGVEAWLPMAEDEMAMLPVELLSLV 415
>ref|XP_002514981.1| Salutaridinol 7-O-acetyltransferase, putative [Ricinus communis]
gi|223546032|gb|EEF47535.1| Salutaridinol
7-O-acetyltransferase, putative [Ricinus communis]
Length = 430
Score = 214 bits (544), Expect = 4e-53
Identities = 126/329 (38%), Positives = 195/329 (59%), Gaps = 17/329 (5%)
Frame = +2
Query: 2 SFVECNDRGVKFVEAQVHA-RLSQVIDEPQMEELKQLVPMDSFEHSESAILAVKTNVFDC 178
+ V+CND GV F++AQ A RLS ++ P E ++L+P + E E A ++ N+FDC
Sbjct: 85 NLVDCNDEGVPFLQAQAIACRLSDAVNNPMPTEFRKLLPFELDEPQEFAF-GIQLNIFDC 143
Query: 179 GGMAVGVCLSHKVADFASSVVFMDAWASMCRGESS--TQPLFDLALHFPPRDSTLSNFLP 352
GG+ + CLSHK+AD S++VF+ WA++ RGE+ P F A FPP++ LS F P
Sbjct: 144 GGICIAFCLSHKIADALSTLVFLKTWAAITRGETDDIVCPEFISATLFPPKN--LSGFNP 201
Query: 353 ----DNENNKTQRFVFDKEKLEMLQRR--TSSKEVEN-----PTKVEALTVFLSQQFIEA 499
EN T+RFVF +E L + TS+ +EN P+++EAL+VF+ +F+ A
Sbjct: 202 AIGITKENVVTKRFVFRLSSIESLIEKYSTSTTSIENQQQKSPSRIEALSVFIWSRFMAA 261
Query: 500 TKTISSTQNKFLIHHAMNLRSRTIPPIPDHTIGNCITSFHAIITSD--DSSDLMISKLRA 673
TK S +L+ HA+NLR+R P +P+H+ GN I +SD + S ++ ++R
Sbjct: 262 TKVESEPGRVYLMLHAVNLRTRMNPQLPEHSFGNYYRVAITIPSSDTEEESYNLVRRMRD 321
Query: 674 AKNSVDNNFIKEVINTKEYLGTIDKLTELVMSGA-ESLIFTSLWRYPVYDVDFGWGKPIW 850
+ + +D +++K++ + E+L I + E G +L FTSL R+P+Y+ DFGW KPIW
Sbjct: 322 SISKIDKDYVKKLQDGNEHLHFIKERAESFTRGEMATLNFTSLCRFPLYEADFGWSKPIW 381
Query: 851 VSDSTVASKNTITFTTTSSKDGIEAWINM 937
++ KN + F T S DGIEA I++
Sbjct: 382 AGAPSLTFKNLVVFMDTVSGDGIEALIHL 410
>ref|XP_002875299.1| transferase family protein [Arabidopsis lyrata subsp. lyrata]
gi|297321137|gb|EFH51558.1| transferase family protein
[Arabidopsis lyrata subsp. lyrata]
Length = 442
Score = 212 bits (540), Expect = 1e-52
Identities = 125/338 (36%), Positives = 199/338 (58%), Gaps = 19/338 (5%)
Frame = +2
Query: 8 VECNDRGVKFVEAQVHARLSQVIDEPQMEELKQLVP--MDSFEHSESAILAVKTNVFDCG 181
++CND G F+EA+V++ LS ++ EP + L+QL+P +DS E LA + + F+CG
Sbjct: 83 IDCNDSGADFLEAEVNSPLSSLLQEPSSDSLQQLIPTSVDSIETRTRLFLA-QASFFECG 141
Query: 182 GMAVGVCLSHKVADFASSVVFMDAWASMC-RGESSTQ--PLFDLALHFPPRDSTLSNFLP 352
MA+GVC+SHK+AD S +FM +WA++ +G T P+FD A FPP + + ++ P
Sbjct: 142 SMAIGVCISHKLADATSIGLFMKSWAAISSQGSIKTVGFPVFDTAKIFPPGNFSETSPAP 201
Query: 353 DNEN----NKT--QRFVFDKEKLEMLQRRTSSKEVENPTKVEALTVFLSQQFIEATKTIS 514
E N+T +RFVFD ++ LQ + SS EV PT+VEA++ + + ++AT+T+S
Sbjct: 202 VVEPEIMMNQTLSKRFVFDSSSIQALQAKASSFEVNQPTRVEAVSALIWKTAMKATRTVS 261
Query: 515 STQNKFLIHHAMNLRSRTIPPIPDHTIGNCITSFHAII---TSDDSSDLMISKLRAAKNS 685
T ++ ++ +LRSR PP ++IGN ++ F A T+ ++SK+R AK
Sbjct: 262 GTSKPSILANSASLRSRVSPPFTKNSIGNLVSYFAAKAEEGTNQTKLQTLVSKIRKAKQW 321
Query: 686 VDNNFIKEVI---NTKEYLGTIDK-LTELVMSG-AESLIFTSLWRYPVYDVDFGWGKPIW 850
+N I +++ N E + + K +++ SG + IF+S R+ +YD DFGWGKP+W
Sbjct: 322 FRDNHIPKLVGNPNATEIICSYQKEAGDMIASGDFDFYIFSSACRFGLYDTDFGWGKPVW 381
Query: 851 VSDSTVASKNTITFTTTSSKDGIEAWINMPPDFLKMIE 964
V TV KN +T T GIEAW+N+ + + E
Sbjct: 382 VGIPTVRQKNIVTLLDTKEAGGIEAWVNLYEQEMNLFE 419
>ref|XP_003538872.1| PREDICTED: vinorine synthase-like [Glycine max]
Length = 433
Score = 207 bits (526), Expect = 5e-51
Identities = 120/340 (35%), Positives = 189/340 (55%), Gaps = 10/340 (2%)
Frame = +2
Query: 2 SFVECNDRGVKFVEAQVHARLSQVIDEPQMEELKQLVPMDSFEHSESAILAVKTNVFDCG 181
+F+ECND G ++EA+V +L+ V++ P E+ L+P + ++ L V+ NVF+CG
Sbjct: 89 AFIECNDEGALYLEAKVRCKLNDVVESPIPNEVTNLLPFGMDDIVDTP-LGVQLNVFECG 147
Query: 182 GMAVGVCLSHKVADFASSVVFMDAWASMCRGESSTQPLFDLALHFPPRDSTL--SNFLPD 355
G+A+G C+SHK+AD S VF+ WA++ R + + F A FPPRD N
Sbjct: 148 GIAIGACMSHKIADAMSFFVFIQTWAAIARDYNEIKTHFVSASLFPPRDIPWYDPNKTIT 207
Query: 356 NENNKTQRFVFDKEKLEMLQRRTSSKEV--ENPTKVEALTVFLSQQFIEATKTISSTQNK 529
N ++ FVFD ++ L+ + + K + P++VEAL+ F+ +F+ +T+ +S +K
Sbjct: 208 KPNTVSRIFVFDASVIDGLKAKYAEKMALQKPPSRVEALSTFIWTRFMASTQVAASESSK 267
Query: 530 F-LIHHAMNLRSRTIPPIPDHTIGNCITSFHAIITSDDSSDL--MISKLRAAKNSVDNNF 700
F ++ H +NLRSR PP+P H GN + A + DD + ++ KLR +DN +
Sbjct: 268 FYVVAHTVNLRSRMDPPLPAHAFGNYYRAVKAFPSLDDKGECYELVEKLREEIRKIDNEY 327
Query: 701 IKEVINTKEYLGTID---KLTELVMSGAESLIFTSLWRYPVYDVDFGWGKPIWVSDSTVA 871
I ++ EYL ++ + E + FT+L R+PVYD DFGWGKPIW
Sbjct: 328 ILKLQEGSEYLSSLREDLRRFENIKGEIVPFTFTALCRFPVYDADFGWGKPIWACPPAWK 387
Query: 872 SKNTITFTTTSSKDGIEAWINMPPDFLKMIEINYNKLLLN 991
KN + FT T GIEA I+M + + + N +LLL+
Sbjct: 388 VKNVVVFTDTKFGGGIEAHISMMEEDMARFQ-NDKELLLH 426