BLASTX nr result
ID: Scutellaria24_contig00012796
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Scutellaria24_contig00012796
(1638 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AEZ55689.1| kaurene synthase 2, partial [Salvia miltiorrhiza] 671 0.0
gb|AEF33360.1| ent-kaurene synthase [Scoparia dulcis] 622 e-177
gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima] 528 e-149
emb|CBI24150.3| unnamed protein product [Vitis vinifera] 520 e-147
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis] 512 e-144
>gb|AEZ55689.1| kaurene synthase 2, partial [Salvia miltiorrhiza]
Length = 762
Score = 671 bits (1731), Expect(2) = 0.0
Identities = 337/493 (68%), Positives = 390/493 (79%), Gaps = 4/493 (0%)
Frame = +2
Query: 59 GDDEIFMDASTCALAFRMLRMNGYNVASDPITKFLEECTSSSSCGNMKDINTTLELYRAS 238
G++EIFMDASTCALAFR LR+NGY+V SDP+TK L+EC SSS GNM DINTTLELYRAS
Sbjct: 273 GNEEIFMDASTCALAFRTLRLNGYDVTSDPVTKILQECFSSSFRGNMTDINTTLELYRAS 332
Query: 239 EFILYPDERDLEKQNLRLKHLLXXXXXXXXXXXXXXXRNID----AEVNHALEYPFYAIL 406
E +LYPDERDLEKQNLRLK LL R+I+ ++VN A+EYPFYAI+
Sbjct: 333 ELVLYPDERDLEKQNLRLKLLLEQELSSGLIQSCQLGRSINVLLISQVNQAIEYPFYAIM 392
Query: 407 DRIANRRNIEHYIIDNTRILKTSYCSPNFGNKDILSLSVEDFNQCQAMHREELRELERWI 586
DR+A R++IE Y DNTRILKTSYCSPNFGN+D LS+EDFN+CQA HREEL ELERW+
Sbjct: 393 DRVAKRKSIEIYNFDNTRILKTSYCSPNFGNEDFHFLSIEDFNRCQAAHREELGELERWV 452
Query: 587 VENKLEELKFARNKSAYCYFSAAATFFPPELSDARMSWAKNSVLXXXXXXXXXXXXSMEE 766
VEN+L+ELKFAR+KSAYCYFSAAATFF PEL DAR+SWAKN VL S+EE
Sbjct: 453 VENRLDELKFARSKSAYCYFSAAATFFAPELLDARLSWAKNGVLTTVIDDFFDVGGSVEE 512
Query: 767 LKNLIQLVEKWDADVSIECVSQNVQIIYTALRRTICEIGDKGFKLQQRSITNHIIEIWLD 946
LKNLIQLVE WD D+ EC S NVQII++ALRRTICEIGDK FKLQ R ITNHII IWLD
Sbjct: 513 LKNLIQLVELWDVDICTECYSHNVQIIFSALRRTICEIGDKAFKLQGRCITNHIIAIWLD 572
Query: 947 LLYSMMKEAEWARENYVPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVEHSEYHT 1126
LL SMM+E EWAR+N+VPT+DEYMSNA+VSFALGPIVLPALYLVGPKLSE+MV HSEYH
Sbjct: 573 LLNSMMRETEWARDNFVPTIDEYMSNAHVSFALGPIVLPALYLVGPKLSEDMVNHSEYHN 632
Query: 1127 LFKLMSTSGRLLNDMRSCEREIKDGKLNAIPIYLINSGGEMSKEAATSEMKSIIESQXXX 1306
LFKLMST GRLLND+ ERE+KDGKLNA+ +Y+IN GGE+SKEAA EMKS IE+Q
Sbjct: 633 LFKLMSTCGRLLNDIHGYERELKDGKLNALSLYIINHGGEVSKEAAIWEMKSWIETQRRE 692
Query: 1307 XXXXXXXXXNGVLPKSCKKLFWHMSTVLHLFYSKDDGFTSQDLIKVVNEVIHQPIVLKEF 1486
VLPK C++LFWHM +V+HLFYSK DGFTSQDLI++VN +IHQPI+L +
Sbjct: 693 LLRLVLEGKKSVLPKPCRELFWHMCSVVHLFYSKGDGFTSQDLIQLVNTIIHQPILLND- 751
Query: 1487 *GINRPGPGISSV 1525
+ G G+S +
Sbjct: 752 ----QTGAGLSKL 760
Score = 32.0 bits (71), Expect(2) = 0.0
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +3
Query: 3 GISHYFQEEIQRVLDETHR 59
GIS YF EIQ VLDET+R
Sbjct: 250 GISQYFSTEIQNVLDETYR 268
>gb|AEF33360.1| ent-kaurene synthase [Scoparia dulcis]
Length = 790
Score = 622 bits (1603), Expect(2) = e-177
Identities = 310/475 (65%), Positives = 370/475 (77%), Gaps = 1/475 (0%)
Frame = +2
Query: 62 DDEIFMDASTCALAFRMLRMNGYNVASDPITKFLEECTSSSSC-GNMKDINTTLELYRAS 238
D+ IFMDA TCALAFR+LRMNGY+V SD I+K LEE SS G++KDI+ +ELY+ S
Sbjct: 317 DEGIFMDACTCALAFRILRMNGYDVTSDQISKVLEEDWHPSSFRGHVKDISMGIELYKPS 376
Query: 239 EFILYPDERDLEKQNLRLKHLLXXXXXXXXXXXXXXXRNIDAEVNHALEYPFYAILDRIA 418
E I+ PDE LEKQ+L LKHL+ RN+D E NH L+YPFY +++R+A
Sbjct: 377 ELIVSPDESILEKQHLELKHLIEKEILKGPVYSSHLVRNVDQEANHVLQYPFYTLMERMA 436
Query: 419 NRRNIEHYIIDNTRILKTSYCSPNFGNKDILSLSVEDFNQCQAMHREELRELERWIVENK 598
NRRNIEHY +D TR+LKTSY SPNFGN+D L+LSV DFN CQ +HREEL+E+ERW+VEN+
Sbjct: 437 NRRNIEHYNLDYTRVLKTSYSSPNFGNRDFLTLSVADFNNCQELHREELKEIERWVVENR 496
Query: 599 LEELKFARNKSAYCYFSAAATFFPPELSDARMSWAKNSVLXXXXXXXXXXXXSMEELKNL 778
+ELKFAR KSAYCYFSAAAT F PELSDARMSWAKN VL SMEEL+NL
Sbjct: 497 SDELKFARQKSAYCYFSAAATIFSPELSDARMSWAKNGVLTTVVDDFFDGGGSMEELRNL 556
Query: 779 IQLVEKWDADVSIECVSQNVQIIYTALRRTICEIGDKGFKLQQRSITNHIIEIWLDLLYS 958
+ LVE+WD DVS C SQNV IIY+ALRRTICEIGD+ F Q+R++T HII+IWLDLL S
Sbjct: 557 VHLVERWDIDVSTGCSSQNVPIIYSALRRTICEIGDQAFLRQERNVTQHIIDIWLDLLNS 616
Query: 959 MMKEAEWARENYVPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVEHSEYHTLFKL 1138
+KEAEW+ E +PTMDEYMSNAYVSFALGPIVLP LYLVGPKLSEEM+ H+EYH LF+L
Sbjct: 617 TLKEAEWSGEKPMPTMDEYMSNAYVSFALGPIVLPPLYLVGPKLSEEMITHAEYHNLFRL 676
Query: 1139 MSTSGRLLNDMRSCEREIKDGKLNAIPIYLINSGGEMSKEAATSEMKSIIESQXXXXXXX 1318
MST GRLLND+R+ ERE KDG NA+ +Y+ SGG+M+KEAA +EMKS+IESQ
Sbjct: 677 MSTVGRLLNDIRTSERERKDGTPNALTLYI--SGGKMTKEAAIAEMKSLIESQRRELLRL 734
Query: 1319 XXXXXNGVLPKSCKKLFWHMSTVLHLFYSKDDGFTSQDLIKVVNEVIHQPIVLKE 1483
N VLPK+CK LFWHM+TVLH+FYSKDDG TSQ++I+VVNE+I+QPIVL E
Sbjct: 735 VLDRKNSVLPKACKDLFWHMNTVLHVFYSKDDGSTSQEMIRVVNEIIYQPIVLDE 789
Score = 27.7 bits (60), Expect(2) = e-177
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = +3
Query: 3 GISHYFQEEIQRVLDETH 56
GI YF+ EIQ VLDET+
Sbjct: 293 GIGQYFRTEIQCVLDETY 310
>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
Length = 784
Score = 528 bits (1359), Expect(2) = e-149
Identities = 259/475 (54%), Positives = 344/475 (72%)
Frame = +2
Query: 59 GDDEIFMDASTCALAFRMLRMNGYNVASDPITKFLEECTSSSSCGNMKDINTTLELYRAS 238
G++EIF+D +TCA+AFR+LR+NG++++SDP T+ E+ SSS G MKDI + LEL+RAS
Sbjct: 309 GEEEIFLDTATCAMAFRILRVNGFDISSDPFTQLSEDHFSSSLGGYMKDIGSVLELFRAS 368
Query: 239 EFILYPDERDLEKQNLRLKHLLXXXXXXXXXXXXXXXRNIDAEVNHALEYPFYAILDRIA 418
+ I++PDE LEKQN L + + EV+ AL++P++A L+R++
Sbjct: 369 QIIIHPDEFVLEKQNFWTSQFLIQELSNGSIHADGLNKYVSQEVDDALKFPYHASLERLS 428
Query: 419 NRRNIEHYIIDNTRILKTSYCSPNFGNKDILSLSVEDFNQCQAMHREELRELERWIVENK 598
NRR IE+Y +NTR+LKT+Y S N GN+D L+L+VEDFN CQ++ REEL++L RWI EN+
Sbjct: 429 NRRAIENYNKNNTRVLKTAYSSSNIGNEDFLNLAVEDFNICQSIQREELKDLARWITENR 488
Query: 599 LEELKFARNKSAYCYFSAAATFFPPELSDARMSWAKNSVLXXXXXXXXXXXXSMEELKNL 778
L++LKFAR K AYCYFSAAAT F PELSDAR+SWAKN VL S+EEL NL
Sbjct: 489 LDKLKFARQKLAYCYFSAAATLFSPELSDARISWAKNGVLTTVVDDFFDVGGSVEELVNL 548
Query: 779 IQLVEKWDADVSIECVSQNVQIIYTALRRTICEIGDKGFKLQQRSITNHIIEIWLDLLYS 958
IQLVEKWD DVS +C S+NV+II++AL TIC+ DKG LQ R++ +HII+IWL+LL S
Sbjct: 549 IQLVEKWDVDVSTDCCSENVEIIFSALHSTICDFADKGLTLQGRNVISHIIDIWLNLLKS 608
Query: 959 MMKEAEWARENYVPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVEHSEYHTLFKL 1138
M+KEAEW R+ VP+MDEYM+N YVSFALGPIVLPALY VGPKLSEE+V E H L+++
Sbjct: 609 MLKEAEWLRDKSVPSMDEYMTNGYVSFALGPIVLPALYCVGPKLSEEIVGTPELHHLYEI 668
Query: 1139 MSTSGRLLNDMRSCEREIKDGKLNAIPIYLINSGGEMSKEAATSEMKSIIESQXXXXXXX 1318
MST GRLLND+++ +RE ++GKLNA+ + +I+ GG+ +KE E+KS I +
Sbjct: 669 MSTCGRLLNDIQTFKRESEEGKLNAVSLCMIHGGGDCTKEETIKELKSFIAGKRRELLKL 728
Query: 1319 XXXXXNGVLPKSCKKLFWHMSTVLHLFYSKDDGFTSQDLIKVVNEVIHQPIVLKE 1483
V+P++CK LFW M VLHLFY KDDGFTS ++ VN V+ +PIVL +
Sbjct: 729 VLQEKGSVVPRACKDLFWKMIKVLHLFYMKDDGFTSHEMFNSVNAVLEEPIVLNK 783
Score = 27.7 bits (60), Expect(2) = e-149
Identities = 11/19 (57%), Positives = 16/19 (84%)
Frame = +3
Query: 3 GISHYFQEEIQRVLDETHR 59
GI +F++EI+ VLDET+R
Sbjct: 286 GIDRHFRKEIKSVLDETYR 304
>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
Length = 826
Score = 520 bits (1340), Expect(2) = e-147
Identities = 259/475 (54%), Positives = 335/475 (70%)
Frame = +2
Query: 62 DDEIFMDASTCALAFRMLRMNGYNVASDPITKFLEECTSSSSCGNMKDINTTLELYRASE 241
++EIF D +TCA+AFR+LR+NGY+++S P+ +F E+ + KD+ LEL+RASE
Sbjct: 352 EEEIFADRATCAIAFRILRLNGYDISSVPLAQFAEDDQYFKFGQDFKDLGAALELFRASE 411
Query: 242 FILYPDERDLEKQNLRLKHLLXXXXXXXXXXXXXXXRNIDAEVNHALEYPFYAILDRIAN 421
I++PDE LEKQN H L + I EV AL +P+YA LDRIAN
Sbjct: 412 MIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQEVEDALRFPYYANLDRIAN 471
Query: 422 RRNIEHYIIDNTRILKTSYCSPNFGNKDILSLSVEDFNQCQAMHREELRELERWIVENKL 601
RR+IEHY +D+TRILKT+Y S + NKD L L+VEDFN CQ++H+ EL++LERWI+EN+L
Sbjct: 472 RRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQSIHQNELKQLERWIIENRL 531
Query: 602 EELKFARNKSAYCYFSAAATFFPPELSDARMSWAKNSVLXXXXXXXXXXXXSMEELKNLI 781
++LKFAR K AYCYFSAAAT F PE SDAR+SWAKNSVL S EEL NLI
Sbjct: 532 DKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLI 591
Query: 782 QLVEKWDADVSIECVSQNVQIIYTALRRTICEIGDKGFKLQQRSITNHIIEIWLDLLYSM 961
QLVEKWD DV+++C S+ V+I+++AL TI EIG K Q R++T+HII+IWL LL SM
Sbjct: 592 QLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSM 651
Query: 962 MKEAEWARENYVPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVEHSEYHTLFKLM 1141
++EA+W PTMDEYM+NAYVSFALGPIVLPALY VGPKLSEE+VE E H L+KLM
Sbjct: 652 LQEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLM 711
Query: 1142 STSGRLLNDMRSCEREIKDGKLNAIPIYLINSGGEMSKEAATSEMKSIIESQXXXXXXXX 1321
ST GRLLND+ S +RE K+GK NA+ +++I+ G ++E A EMK +++SQ
Sbjct: 712 STCGRLLNDIHSFKRESKEGKANALALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLV 771
Query: 1322 XXXXNGVLPKSCKKLFWHMSTVLHLFYSKDDGFTSQDLIKVVNEVIHQPIVLKEF 1486
+P+ CK LFW MS VLH FY KDDGFTS D+++ V VI++P++L EF
Sbjct: 772 LQEKGSTVPRICKDLFWKMSKVLHTFYEKDDGFTSHDMLRAVKSVIYEPVLLAEF 826
Score = 28.5 bits (62), Expect(2) = e-147
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +3
Query: 3 GISHYFQEEIQRVLDETHR 59
GI YF+ EI+ VLDET+R
Sbjct: 328 GIDRYFRMEIRSVLDETYR 346
>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
Length = 737
Score = 512 bits (1319), Expect(2) = e-144
Identities = 258/475 (54%), Positives = 341/475 (71%)
Frame = +2
Query: 59 GDDEIFMDASTCALAFRMLRMNGYNVASDPITKFLEECTSSSSCGNMKDINTTLELYRAS 238
GD++I DA+TCA+AFR+LR+NGY+V++DP+T++ E+ +S G MKDI+ LEL+RAS
Sbjct: 263 GDEDILSDAATCAMAFRLLRVNGYDVSADPLTQYSEDRLFNSLGGYMKDIDDALELFRAS 322
Query: 239 EFILYPDERDLEKQNLRLKHLLXXXXXXXXXXXXXXXRNIDAEVNHALEYPFYAILDRIA 418
E I++PDE LEKQ+ H L ++I EV+ AL+ P YAIL R++
Sbjct: 323 ETIIHPDESVLEKQHHWTSHFLKQELSNTLIRGHKLNKHIGLEVDDALKLPRYAILGRLS 382
Query: 419 NRRNIEHYIIDNTRILKTSYCSPNFGNKDILSLSVEDFNQCQAMHREELRELERWIVENK 598
R I++Y D+TRILK+ Y N GN+D L L+V+D N CQ++HREEL L RW+ E +
Sbjct: 383 TRTAIKYYNTDSTRILKSYYRCLNIGNEDFLKLAVDDLNNCQSIHREELNHLTRWVEEYR 442
Query: 599 LEELKFARNKSAYCYFSAAATFFPPELSDARMSWAKNSVLXXXXXXXXXXXXSMEELKNL 778
L++L FAR K AYCYFSAAAT FPPELSDAR+SWAKN VL S EEL NL
Sbjct: 443 LDKLNFARQKQAYCYFSAAATLFPPELSDARISWAKNGVLTTVVDDFFDVGGSEEELINL 502
Query: 779 IQLVEKWDADVSIECVSQNVQIIYTALRRTICEIGDKGFKLQQRSITNHIIEIWLDLLYS 958
IQLVEKWD +V ++C S+NV+II++AL+ TI EIG + F Q RS+T+H+IEIWLDL+ S
Sbjct: 503 IQLVEKWDVNVGVDCCSENVEIIFSALKNTINEIGARAFTRQGRSVTSHVIEIWLDLIKS 562
Query: 959 MMKEAEWARENYVPTMDEYMSNAYVSFALGPIVLPALYLVGPKLSEEMVEHSEYHTLFKL 1138
M KEAEW R VPTM+EYM NAYVSFALGPIVLPALYLVGPKLSEE V SE+ L++L
Sbjct: 563 MFKEAEWLRNKSVPTMEEYMENAYVSFALGPIVLPALYLVGPKLSEEAVGSSEFDHLYRL 622
Query: 1139 MSTSGRLLNDMRSCEREIKDGKLNAIPIYLINSGGEMSKEAATSEMKSIIESQXXXXXXX 1318
MST+GRLLND++ +RE +GKLNA+ + +I+ G +++E A +EMK++I S+
Sbjct: 623 MSTAGRLLNDIQGFKRESAEGKLNAVSLAMIHGNG-VTEEEAINEMKNVIVSKRRELLRL 681
Query: 1319 XXXXXNGVLPKSCKKLFWHMSTVLHLFYSKDDGFTSQDLIKVVNEVIHQPIVLKE 1483
V+P++CK LFW+MS VLHLFY+K DGFT+ D++K VN V+ +PI+L E
Sbjct: 682 VLLEKGSVVPRACKDLFWNMSKVLHLFYAKHDGFTAHDMMKTVNAVMEEPILLSE 736
Score = 29.3 bits (64), Expect(2) = e-144
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +3
Query: 3 GISHYFQEEIQRVLDETHR 59
GI +F+EEI+ VLDET+R
Sbjct: 240 GIDRHFREEIRSVLDETYR 258