BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Scutellaria24_contig00010354
         (1972 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]               756   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   738   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   737   0.0  
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   699   0.0  
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   699   0.0  

>gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  756 bits (1952), Expect = 0.0
 Identities = 360/529 (68%), Positives = 435/529 (82%)
 Frame = +2

Query: 2    CEEPAFDWRGEVHPCLPMEKMVIYRLNVTRFTADKSSKLPGDIAGSFSGISAKLQHFKDL 181
            C EP +DW G+V P LPMEK++IYRLNVT+FT DKSSKLP D+AG+FSGIS K  HFKDL
Sbjct: 334  CLEPGYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDL 393

Query: 182  GVNAILLEPAFPFDEQKGPYYPWHFFCPGSLYGPSGDPMTVAKSMKEMVKTLHASGIEVL 361
            GVNA+LLEP FPFDEQKGPY+PWHFF PG++YGPSGDP++  KSMK+MVK LHA+GIEV 
Sbjct: 394  GVNAMLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVF 453

Query: 362  LEVVFTHTSEVAALVEIDNTCYYNVEGGEDLKSKNALNCNHPVVQQLILESLRYWVIEYH 541
            LEVVFTHT+E A L+ +DN  Y  ++GG+ L  +NALNCN+P+VQQ+IL+ LR+WVIE+H
Sbjct: 454  LEVVFTHTAEDAPLMNVDNFSYC-IKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFH 512

Query: 542  IDGFCFINASSLTRGSHGEFLSRPPLVEAIAFDPVLSKVKVIADSWNPHGIETEKIVFPH 721
            IDGF F+NASSL RG +GE LSRPPLVEAIAFDP+LSKVK+IAD+WNP   ++++ +FPH
Sbjct: 513  IDGFVFVNASSLLRGFNGEILSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPH 572

Query: 722  WKRWAEMNAMFYDDVRNFLRGKGLISKLATRVCGSGDLFLGGRGPAFSFNYVTRNVGLTL 901
            W+RWAE+N  F DD+R+FLRG+GL+S LATR+CGSGD+F GGRGPAFSFNY+ RN GLTL
Sbjct: 573  WRRWAEINMRFCDDIRDFLRGEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTL 632

Query: 902  VDLVSFSSDELESQLSWNCGEQGPTNKTVVLETRLKQIRNFLFILFISLGVPVLNMGDEY 1081
            VDLVSFSS+E+ S+LSWNCG++G T   +VLE RLKQ+RNFLFILFISLGVPVLNMGDE 
Sbjct: 633  VDLVSFSSNEVASELSWNCGQEGATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDEC 692

Query: 1082 GQSSRGSLAYADRKPMDWNALRSGFSNQVTQFISFLSTLKIRRSDLLQTRNFLKEDNIEW 1261
            GQSS G  AY  RK + WN L++GF  Q+ QFISFLS L++RRSDLLQ R FLKE+NI+W
Sbjct: 693  GQSSGGPPAYDARKSLGWNTLKTGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQW 752

Query: 1262 HGTNQSPPQWDDSLCKFLAMTLKVDAQSSQSTSGSLNMHGNLFIAFNSADHSEEVSLPPL 1441
            HG++QSPP+WD    KFLAMTLK DA+ SQ+     ++ G+LF+AFN A  SE V LPP 
Sbjct: 753  HGSDQSPPKWDGPSSKFLAMTLKADAEVSQTLVS--DIVGDLFVAFNGAGDSEIVILPPP 810

Query: 1442 AVDRTWVRLVDTALPYPGFFTVDGVPLEDGSVTYEMKSHSCILFEARIL 1588
              D  W RLVDTALP+PGFF   G P+ED  V YEMKSHSC+LFEA+ L
Sbjct: 811  PTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  738 bits (1904), Expect = 0.0
 Identities = 351/531 (66%), Positives = 429/531 (80%), Gaps = 3/531 (0%)
 Frame = +2

Query: 5    EEPAFDWRGEVHPCLPMEKMVIYRLNVTRFTADKSSKLPGDIAGSFSGISAKLQHFKDLG 184
            +EPAF+W  +VHP +PMEK+V+YRLNV  FT D+SS++  D+AG+FSG+  KL HFKDLG
Sbjct: 351  KEPAFNWNDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLG 410

Query: 185  VNAILLEPAFPFDEQKGPYYPWHFFCPGSLYGPSGDPMTVAKSMKEMVKTLHASGIEVLL 364
            VNA+LLEP F FDEQKGPY+P+HFF P ++YGPS  P++   S+KEMVK LHA+GIEVLL
Sbjct: 411  VNAVLLEPIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLL 470

Query: 365  EVVFTHTSEVAALVEIDNTCYYNVEGGEDLKSKNALNCNHPVVQQLILESLRYWVIEYHI 544
            EVVFTHT+E  AL  ID++CYY V G  DL  +NALNCN+ +VQQ+I++SLRYWV E+H+
Sbjct: 471  EVVFTHTAESGALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHV 530

Query: 545  DGFCFINASSLTRGSHGEFLSRPPLVEAIAFDPVLSKVKVIADSWNPHGIETEKIVFPHW 724
            DGFCFINASSL RG HGE+LSRPPLVE IAFDP+LSK K+IAD W+P  +  ++I FPHW
Sbjct: 531  DGFCFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHW 590

Query: 725  KRWAEMNAMFYDDVRNFLRGKGLISKLATRVCGSGDLFLGGRGPAFSFNYVTRNVGLTLV 904
            KRWAE+N  F +DVRNFLRG+GL S  ATR+CGSGD+F+ GRGPAFSFN+ T+N GL LV
Sbjct: 591  KRWAEVNTRFCNDVRNFLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLV 649

Query: 905  DLVSFSSDELESQLSWNCGEQGPTNKTVVLETRLKQIRNFLFILFISLGVPVLNMGDEYG 1084
            DLVSFSS EL S+LSWNCG++GPTNKT VLE RLKQIRNFLFIL++SLGVP+LNMGDE G
Sbjct: 650  DLVSFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECG 709

Query: 1085 QSSRGSLAYADRKPMDWNALRSGFSNQVTQFISFLSTLKIRRSDLLQTRNFLKEDNIEWH 1264
            QSS GS AY DRKP +WN++++GF  Q  QFISFLS+L+ RRSDLLQ R+FLKE++I+WH
Sbjct: 710  QSSGGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWH 769

Query: 1265 GTNQSPPQWDDSLCKFLAMTLKVDAQSSQSTSGSLNMHGNLFIAFNSADHSEEVSLPPLA 1444
            G++QSPP+WDD   KFLAMTLK +    Q  S S ++ G+LFIAFN+AD S +V LPP  
Sbjct: 770  GSDQSPPRWDDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPP 829

Query: 1445 VDRTWVRLVDTALPYPGFFTVDGVPL---EDGSVTYEMKSHSCILFEARIL 1588
                W RLVDTALP+PGFFT DG  +   + G VTY+M+SHSC LFEA  L
Sbjct: 830  TGMVWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTL 880


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  737 bits (1902), Expect = 0.0
 Identities = 350/532 (65%), Positives = 426/532 (80%), Gaps = 3/532 (0%)
 Frame = +2

Query: 2    CEEPAFDWRGEVHPCLPMEKMVIYRLNVTRFTADKSSKLPGDIAGSFSGISAKLQHFKDL 181
            CEEPAF+W  ++ P L MEK+++YRLNV RFT  KS KL  DIAG+F+G+  K+ HF++L
Sbjct: 341  CEEPAFEWGSDIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNL 400

Query: 182  GVNAILLEPAFPFDEQKGPYYPWHFFCPGSLYGPSGDPMTVAKSMKEMVKTLHASGIEVL 361
            GVNA+LLEP FPFDEQKGPY+P+HFF P ++YGPSG  ++   SMKEMVK LHA+ IEVL
Sbjct: 401  GVNAVLLEPIFPFDEQKGPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVL 460

Query: 362  LEVVFTHTSEVAALVEIDNTCYYNVEGGEDLKSKNALNCNHPVVQQLILESLRYWVIEYH 541
            LEVVFTHT+E  AL  ID+  YY  +   D  S+NALNCN+P+VQ++IL+SL++WV E+H
Sbjct: 461  LEVVFTHTAEGGALQGIDDFSYYYTKSSMD--SRNALNCNYPIVQRMILDSLQHWVTEFH 518

Query: 542  IDGFCFINASSLTRGSHGEFLSRPPLVEAIAFDPVLSKVKVIADSWNPHGIETEKIVFPH 721
            IDGFCFINAS+L  G HGE LSRPPLVEAIAFDP+LSK K+IAD W+P     ++  FPH
Sbjct: 519  IDGFCFINASALLTGFHGEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPH 578

Query: 722  WKRWAEMNAMFYDDVRNFLRGKGLISKLATRVCGSGDLFLGGRGPAFSFNYVTRNVGLTL 901
            WKRWAE+N  F  DVRNFLRG+ L+  LATR+CGSGD+F  GRGPAFSFNY+ RN GL L
Sbjct: 579  WKRWAEINPKFCIDVRNFLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPL 638

Query: 902  VDLVSFSSDELESQLSWNCGEQGPTNKTVVLETRLKQIRNFLFILFISLGVPVLNMGDEY 1081
            VDLVSFS  EL S+LSWNCGE+GPTNKT VLE RLKQIRN+LFIL++SLGVPVLNMGDE 
Sbjct: 639  VDLVSFSGGELGSELSWNCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDEC 698

Query: 1082 GQSSRGSLAYADRKPMDWNALRSGFSNQVTQFISFLSTLKIRRSDLLQTRNFLKEDNIEW 1261
            GQSSRGS++Y DRKP DWNAL + F NQ+TQFISFLS+L++RRSDLLQ RNFLKE+NI+W
Sbjct: 699  GQSSRGSISYGDRKPFDWNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDW 758

Query: 1262 HGTNQSPPQWDDSLCKFLAMTLKVDAQSSQSTSGSLNMHGNLFIAFNSADHSEEVSLPPL 1441
            HG +QSPP+W+D  CKFLAMTLK+D   SQ +S   N+ G+LF+AFN+A H+E V LPP+
Sbjct: 759  HGNDQSPPRWEDPTCKFLAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPV 818

Query: 1442 AVDRTWVRLVDTALPYPGFFTVDGVPLED---GSVTYEMKSHSCILFEARIL 1588
                 W RLVDTALP+PGFF+ DG P+ +   G + Y+M SHSC LFEA I+
Sbjct: 819  PEGMIWRRLVDTALPFPGFFSEDGEPVVEQIAGLIAYKMNSHSCTLFEAGIM 870


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  699 bits (1805), Expect = 0.0
 Identities = 327/526 (62%), Positives = 418/526 (79%), Gaps = 2/526 (0%)
 Frame = +2

Query: 11   PAFDWRGEVHPCLPMEKMVIYRLNVTRFTADKSSKLPGDIAGSFSGISAKLQHFKDLGVN 190
            P FDW GEVHP LPMEK+ +YRLNV RFT DKSS+LP DIAG+FSG++ KL HFK+LGVN
Sbjct: 357  PTFDWDGEVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 416

Query: 191  AILLEPAFPFDEQKGPYYPWHFFCPGSLYGPSGDPMTVAKSMKEMVKTLHASGIEVLLEV 370
            A+LLEP F FDE++GPY+P+HFF P + YGPSG  ++   SMKEMVK LHA+G+EV+LEV
Sbjct: 417  AVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEV 476

Query: 371  VFTHTSEVAALVEIDNTCYYNVEGGEDLKSKNALNCNHPVVQQLILESLRYWVIEYHIDG 550
            V+THTS   AL  ID++ YY      +L+ K+ALNCN+P+VQQL+L+SLRYWV E+H+DG
Sbjct: 477  VYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDG 536

Query: 551  FCFINASSLTRGSHGEFLSRPPLVEAIAFDPVLSKVKVIADSWNPHGIETEKIVFPHWKR 730
            FCF+NAS L RG HGE LSRPP VEAIAFDP+LSK K++AD W+P  +E+++  FPHWKR
Sbjct: 537  FCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 596

Query: 731  WAEMNAMFYDDVRNFLRGKGLISKLATRVCGSGDLFLGGRGPAFSFNYVTRNVGLTLVDL 910
            WAE+N+ F  D+R+F RG+GLIS LATR+CGSGD+F  GRGPAFSFN++ RNVGL LVDL
Sbjct: 597  WAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 656

Query: 911  VSFSSDELESQLSWNCGEQGPTNKTVVLETRLKQIRNFLFILFISLGVPVLNMGDEYGQS 1090
            VSFS+  L S+LSWNCGE+GPT+   VLE RLKQIRNF+F+LF+SLGVPVLNMGDE GQS
Sbjct: 657  VSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 716

Query: 1091 SRGSLAYADRKPMDWNALRSGFSNQVTQFISFLSTLKIRRSDLLQTRNFLKEDNIEWHGT 1270
            S GS+A+ D++  +W+ L++ F  Q TQFI+FLS+ + RR DL Q RNFLK +NI+W   
Sbjct: 717  SGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDN 776

Query: 1271 NQSPPQWDDSLCKFLAMTLKVDAQSSQSTSGSLNMHGNLFIAFNSADHSEEVSLPPLAVD 1450
            NQSPPQW+D+ CKFLA+ L+ D + ++S + +     N+F+ FN++D SE V+LP     
Sbjct: 777  NQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEG 836

Query: 1451 RTWVRLVDTALPYPGFFTVDG--VPLEDGSVTYEMKSHSCILFEAR 1582
             +W R+VDTALP+PGFF+ DG  VP+  GSVTYE+++HSC LFEA+
Sbjct: 837  TSWFRVVDTALPFPGFFSSDGELVPM-TGSVTYEIQAHSCALFEAK 881


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  699 bits (1805), Expect = 0.0
 Identities = 327/526 (62%), Positives = 418/526 (79%), Gaps = 2/526 (0%)
 Frame = +2

Query: 11   PAFDWRGEVHPCLPMEKMVIYRLNVTRFTADKSSKLPGDIAGSFSGISAKLQHFKDLGVN 190
            P FDW GEVHP LPMEK+ +YRLNV RFT DKSS+LP DIAG+FSG++ KL HFK+LGVN
Sbjct: 357  PTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVN 416

Query: 191  AILLEPAFPFDEQKGPYYPWHFFCPGSLYGPSGDPMTVAKSMKEMVKTLHASGIEVLLEV 370
            A+LLEP F FDE++GPY+P+HFF P + YGPSG  ++   SMKEMVK LHA+G+EV+LEV
Sbjct: 417  AVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEV 476

Query: 371  VFTHTSEVAALVEIDNTCYYNVEGGEDLKSKNALNCNHPVVQQLILESLRYWVIEYHIDG 550
            V+THTS   AL  ID++ YY      +L+ K+ALNCN+P+VQQL+L+SLRYWV E+H+DG
Sbjct: 477  VYTHTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDG 536

Query: 551  FCFINASSLTRGSHGEFLSRPPLVEAIAFDPVLSKVKVIADSWNPHGIETEKIVFPHWKR 730
            FCF+NAS L RG HGE LSRPP VEAIAFDP+LSK K++AD W+P  +E+++  FPHWKR
Sbjct: 537  FCFVNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKR 596

Query: 731  WAEMNAMFYDDVRNFLRGKGLISKLATRVCGSGDLFLGGRGPAFSFNYVTRNVGLTLVDL 910
            WAE+N+ F  D+R+F RG+GLIS LATR+CGSGD+F  GRGPAFSFN++ RNVGL LVDL
Sbjct: 597  WAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDL 656

Query: 911  VSFSSDELESQLSWNCGEQGPTNKTVVLETRLKQIRNFLFILFISLGVPVLNMGDEYGQS 1090
            VSFS+  L S+LSWNCGE+GPT+   VLE RLKQIRNF+F+LF+SLGVPVLNMGDE GQS
Sbjct: 657  VSFSNSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQS 716

Query: 1091 SRGSLAYADRKPMDWNALRSGFSNQVTQFISFLSTLKIRRSDLLQTRNFLKEDNIEWHGT 1270
            S GS+A+ D++  +W+ L++ F  Q TQFI+FLS+ + RR DL Q RNFLK +NI+W   
Sbjct: 717  SGGSVAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDN 776

Query: 1271 NQSPPQWDDSLCKFLAMTLKVDAQSSQSTSGSLNMHGNLFIAFNSADHSEEVSLPPLAVD 1450
            NQSPPQW+D+ CKFLA+ L+ D + ++S + +     N+F+ FN++D SE V+LP     
Sbjct: 777  NQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEG 836

Query: 1451 RTWVRLVDTALPYPGFFTVDG--VPLEDGSVTYEMKSHSCILFEAR 1582
             +W R+VDTALP+PGFF+ DG  VP+  GSVTYE+++HSC LFEA+
Sbjct: 837  TSWFRVVDTALPFPGFFSSDGELVPM-TGSVTYEIQAHSCALFEAK 881