BLASTX nr result
ID: Scutellaria24_contig00002131
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Scutellaria24_contig00002131
(2113 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec... 874 0.0
dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans] 872 0.0
sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,... 870 0.0
gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus lon... 867 0.0
gb|AFP93564.1| GCS [Cestrum nocturnum] 865 0.0
>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
ligase, chloroplast precursor, putative [Ricinus
communis]
Length = 526
Score = 874 bits (2258), Expect = 0.0
Identities = 424/523 (81%), Positives = 467/523 (89%), Gaps = 4/523 (0%)
Frame = +1
Query: 97 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNLCSPSWSSLKVNKSW 276
M ++S + + ++I + K +N V N++E+ + KE S S +S K+ +
Sbjct: 1 MPVISQAGPSCYVPTDITRCKTGQNAVLGVFNSIETSKLKETCVRFSSLSCNSTKIPQLP 60
Query: 277 SS----VGQRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEK 444
SS V RRG ++ VAASPP EDAVIATEPLTKEDLV YLASGCKPK+ WRIGTEHEK
Sbjct: 61 SSENSWVRSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEK 120
Query: 445 FGFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSG 624
FGFE+GTLRPMKYEQI+ELLNGI+ERFDW+K+MEG+YIIGLKQGKQSISLEPGGQFELSG
Sbjct: 121 FGFELGTLRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSG 180
Query: 625 APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYM 804
APLETLHQTCAEVNSHLYQVKAV EEMGIGFLGIGFQPKW +DIP+MPKGRYEIMRNYM
Sbjct: 181 APLETLHQTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYM 240
Query: 805 PKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDY 984
PKVG+LGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN Y
Sbjct: 241 PKVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGY 300
Query: 985 LSMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD 1164
LSMRSQIWTDTD +R GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC G++FRD
Sbjct: 301 LSMRSQIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRD 360
Query: 1165 FMAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL 1344
F+AGKL +PGE PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+L
Sbjct: 361 FLAGKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLL 420
Query: 1345 YDEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERR 1524
YDE+SLQNVLDMTADWTPEER+MLRNKVPK+GLKTPFRDGLLKHVA++V+ AKDGLERR
Sbjct: 421 YDEISLQNVLDMTADWTPEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERR 480
Query: 1525 GFKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 1653
G+KE GFLNEVTEVV+TGVTPAE+LLELY+GKWGQSVDPVFEE
Sbjct: 481 GYKEIGFLNEVTEVVRTGVTPAERLLELYNGKWGQSVDPVFEE 523
>dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]
Length = 523
Score = 872 bits (2253), Expect = 0.0
Identities = 429/525 (81%), Positives = 473/525 (90%), Gaps = 6/525 (1%)
Frame = +1
Query: 97 MALMSHPASAQSIHSEIIQLK-GRRNGVNSSANTME-SGRSKEVLFNLCSPSWSSLKVNK 270
M LMS + + IH+EI+Q K G + +N ++NT ++ +V F S + LK +
Sbjct: 1 MVLMSQASPSHGIHAEILQSKSGYSSLLNGASNTNAFRHQTSKVAF-----SRNYLKYTQ 55
Query: 271 SW--SSVGQ--RRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEH 438
+ +VG +RG ++ VAASPPTEDAV+ATEPLTKEDLV YLASGCKPK+NWRIGTEH
Sbjct: 56 AMHVDAVGGNFKRGNKVIVAASPPTEDAVVATEPLTKEDLVGYLASGCKPKENWRIGTEH 115
Query: 439 EKFGFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFEL 618
EKFGF++ TLRPM YEQI+ LLN ISERFDW+K+MEG+ IIGLKQGKQSISLEPGGQFEL
Sbjct: 116 EKFGFDLKTLRPMTYEQIAHLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFEL 175
Query: 619 SGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRN 798
SGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGF+GIGFQPKW R+DIP+MPKGRYEIMRN
Sbjct: 176 SGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFIGIGFQPKWERKDIPIMPKGRYEIMRN 235
Query: 799 YMPKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPN 978
YMPKVGSLGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN
Sbjct: 236 YMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPN 295
Query: 979 DYLSMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSF 1158
YLSMRSQIWTDTDN+R+GMLPFVFDDSFGFEQYV+YALDVPMYFVYRKKKYIDCAGLSF
Sbjct: 296 GYLSMRSQIWTDTDNDRSGMLPFVFDDSFGFEQYVEYALDVPMYFVYRKKKYIDCAGLSF 355
Query: 1159 RDFMAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 1338
RDF+AGKL +PGE+PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG
Sbjct: 356 RDFLAGKLPPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 415
Query: 1339 ILYDEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLE 1518
+LYD++SLQNVLDMTADWT EERQMLRNKVP +GLKTPFRDGLLKHVA+EV+ FAKDGLE
Sbjct: 416 VLYDDISLQNVLDMTADWTQEERQMLRNKVPVAGLKTPFRDGLLKHVAEEVLKFAKDGLE 475
Query: 1519 RRGFKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 1653
RRG+KETGFLNEV EVV+TG+TPAEKLLELYHGKWGQSVDPVFEE
Sbjct: 476 RRGYKETGFLNEVAEVVRTGLTPAEKLLELYHGKWGQSVDPVFEE 520
>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
Full=Gamma-ECS; Short=GCS; AltName:
Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
gi|111380512|gb|ABD98695.2| chloroplast
gamma-glutamylcysteine synthetase [Nicotiana tabacum]
Length = 522
Score = 870 bits (2247), Expect = 0.0
Identities = 431/522 (82%), Positives = 463/522 (88%), Gaps = 3/522 (0%)
Frame = +1
Query: 97 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNLCSPSWSSLKVNKSW 276
MALMS S+ I+SE ++ +G +S + ME + K++ F S SS + +
Sbjct: 1 MALMSQAGSSHCIYSEKMKCI---SGHSSITSNMEMLKMKDICFGNISSRNSSKPMQGIY 57
Query: 277 SS---VGQRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEKF 447
V +RRG VAASPPTEDAV+A EPLTKEDLV YLASGCK K+ WRIGTEHEKF
Sbjct: 58 LDRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKF 117
Query: 448 GFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSGA 627
GFE GTLRPMKYEQI+ELLNGI+ERFDW+K+MEG+ IIGLKQGKQSISLEPGGQFELSGA
Sbjct: 118 GFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGA 177
Query: 628 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYMP 807
PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKW +DIPVMPKGRYEIMRNYMP
Sbjct: 178 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMP 237
Query: 808 KVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDYL 987
KVGSLGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN YL
Sbjct: 238 KVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 297
Query: 988 SMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 1167
SMRS IWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAG+SFRDF
Sbjct: 298 SMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDF 357
Query: 1168 MAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 1347
M GKLS +PG++PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY
Sbjct: 358 MNGKLSPIPGDYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 417
Query: 1348 DEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERRG 1527
DEVSLQ VLDMT+DWT EER+MLRNKVP SGLKTPFRDGLLKHVAQ+VV AK+GLERRG
Sbjct: 418 DEVSLQTVLDMTSDWTAEEREMLRNKVPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRG 477
Query: 1528 FKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 1653
+KETGFLNEVTEVV+TGVTPAEKLLELYHGKWG+SVDPVFEE
Sbjct: 478 YKETGFLNEVTEVVRTGVTPAEKLLELYHGKWGRSVDPVFEE 519
>gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus longan]
Length = 511
Score = 867 bits (2240), Expect = 0.0
Identities = 430/519 (82%), Positives = 458/519 (88%)
Frame = +1
Query: 97 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNLCSPSWSSLKVNKSW 276
MAL+S + + SEI G R GV N +E+ R KE S+SSL +
Sbjct: 1 MALVSQGGATYRVRSEITP--GHR-GVFGLVNNVEASRVKESCV-----SFSSLSC---Y 49
Query: 277 SSVGQRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEKFGFE 456
S+ G RRG R VAASPPT+DAVIA EPLTKEDLV YLASGCKP++ WRIGTEHEKFGFE
Sbjct: 50 STKGSRRGYRAIVAASPPTDDAVIAAEPLTKEDLVGYLASGCKPREKWRIGTEHEKFGFE 109
Query: 457 IGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSGAPLE 636
IGTLRPMKYEQI+ELLN I+ERFDW+KIME IIGLKQGKQSISLEPGGQFELSGAPLE
Sbjct: 110 IGTLRPMKYEQIAELLNSIAERFDWEKIMEDNNIIGLKQGKQSISLEPGGQFELSGAPLE 169
Query: 637 TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYMPKVG 816
TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW +DIPVMPKGRYEIM+NYMPKVG
Sbjct: 170 TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMKNYMPKVG 229
Query: 817 SLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDYLSMR 996
SLGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN YLSMR
Sbjct: 230 SLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMR 289
Query: 997 SQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFMAG 1176
SQIWTDTDNNR GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC G+SFRDF+ G
Sbjct: 290 SQIWTDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFLVG 349
Query: 1177 KLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEV 1356
KL LPGE PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDEV
Sbjct: 350 KLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEV 409
Query: 1357 SLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERRGFKE 1536
SLQN LD+TADWT EERQMLRNKVPK+GLKTPFRDGLL+HVAQ+++ AKDGLERRGFKE
Sbjct: 410 SLQNALDLTADWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAQDILKLAKDGLERRGFKE 469
Query: 1537 TGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 1653
+GFLN V EVV+TGVTPAEKLLE+YHGKWGQSVDPVFEE
Sbjct: 470 SGFLNAVAEVVRTGVTPAEKLLEMYHGKWGQSVDPVFEE 508
>gb|AFP93564.1| GCS [Cestrum nocturnum]
Length = 518
Score = 865 bits (2236), Expect = 0.0
Identities = 432/523 (82%), Positives = 461/523 (88%), Gaps = 4/523 (0%)
Frame = +1
Query: 97 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNL-CSPSWSS-LKVNK 270
MALMS S+ IH E I+ KG N ME + KE+ F + SP SS K
Sbjct: 1 MALMSQAGSSHCIHPEKIRCKGGHN--------MEIFKLKEICFGVHISPRNSSNAKQGI 52
Query: 271 SWSSVG--QRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEK 444
VG +RRG VAASPPT+DAV+A EPLTKEDLV YLASGCK K+ WRIGTEHEK
Sbjct: 53 CLRRVGAERRRGRLSIVAASPPTDDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEK 112
Query: 445 FGFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSG 624
FGFE GTLRPM YEQI+ELL G+SERFDW+K+MEG+ IIGLKQGKQSISLEPGGQFELSG
Sbjct: 113 FGFEFGTLRPMNYEQIAELLYGVSERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSG 172
Query: 625 APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYM 804
APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW +DIPVMPKGRYEIMRNYM
Sbjct: 173 APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYM 232
Query: 805 PKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDY 984
PKVG+LGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPF EGKPN Y
Sbjct: 233 PKVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFKEGKPNGY 292
Query: 985 LSMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD 1164
LSMRS IWTDTDNNR+GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD
Sbjct: 293 LSMRSHIWTDTDNNRSGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD 352
Query: 1165 FMAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL 1344
F+ GKL+ +PGE+PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL
Sbjct: 353 FLNGKLAPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL 412
Query: 1345 YDEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERR 1524
YDE SLQ+VLDMTADWT EER+MLRNKVPKSGLKTPFRDGLLKHVAQ+V+ AK+GLERR
Sbjct: 413 YDEASLQSVLDMTADWTAEEREMLRNKVPKSGLKTPFRDGLLKHVAQDVMKLAKEGLERR 472
Query: 1525 GFKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 1653
G+KETGFLNEVTEVV+TGVTPAEKLL+LYHGKWGQSVDPVFEE
Sbjct: 473 GYKETGFLNEVTEVVRTGVTPAEKLLDLYHGKWGQSVDPVFEE 515