BLASTX nr result
ID: Scutellaria24_contig00000032
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Scutellaria24_contig00000032
(2163 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002269949.1| PREDICTED: phosphoinositide phospholipase C ... 875 0.0
ref|XP_003633437.1| PREDICTED: phosphoinositide phospholipase C ... 874 0.0
ref|XP_002270230.1| PREDICTED: phosphoinositide phospholipase C ... 866 0.0
ref|XP_002311223.1| predicted protein [Populus trichocarpa] gi|2... 845 0.0
ref|XP_002316212.1| predicted protein [Populus trichocarpa] gi|2... 838 0.0
>ref|XP_002269949.1| PREDICTED: phosphoinositide phospholipase C 2 isoform 1 [Vitis
vinifera] gi|147828132|emb|CAN64084.1| hypothetical
protein VITISV_006935 [Vitis vinifera]
Length = 593
Score = 875 bits (2262), Expect = 0.0
Identities = 430/592 (72%), Positives = 477/592 (80%), Gaps = 12/592 (2%)
Frame = +3
Query: 156 TKQTYXXXXXXXXXXXLTASVAPADIKELFTSYSENGVMFADHLQRFLVEVQKHETATVE 335
+KQTY LT S AP +IKELF YSENG+M DHLQRFL+EVQ+ E ATVE
Sbjct: 2 SKQTYRVCFCFRRRFRLTVSEAPQEIKELFDRYSENGMMSIDHLQRFLIEVQQEEKATVE 61
Query: 336 DAQAIIDSL---KHLSMFHRRGLHLEGFFRYLFGDANPPLNPDLGVHHDMTAPLSHYFIY 506
DAQAI+DSL KHL++FHR+ L+LE FF+YLFGD NPPL+ LGVHHDMTAPLSHYF+Y
Sbjct: 62 DAQAILDSLHEFKHLNIFHRKALNLEAFFKYLFGDINPPLSTALGVHHDMTAPLSHYFVY 121
Query: 507 TGHNSYLTGNQLSSDCSDVPIINALHSGVRVIELDIWPNSSNDDVDVLHGRTLTTPVALI 686
TGHNSYLTGNQLSSDCSDVPII+AL GVRVIELDIWPNS+ D+VDVLHGRTLTTPV LI
Sbjct: 122 TGHNSYLTGNQLSSDCSDVPIIHALQRGVRVIELDIWPNSTKDNVDVLHGRTLTTPVELI 181
Query: 687 KCLKSIKEHAFVKSEYPVVITLEDHLTPDLQAKVAEMITQTFEDILFCPGSECLKDFPSP 866
KCLKSIKEHAF SEYPVVITLEDHLTPDLQAKVAEM+TQTF DILF PG ECL +FPSP
Sbjct: 182 KCLKSIKEHAFSASEYPVVITLEDHLTPDLQAKVAEMVTQTFGDILFTPGPECLNEFPSP 241
Query: 867 ESLKRRIIISTKPPKEYLQAXXXXXXXXXXXXGMDATEEEAWGSEIKNF---------SS 1019
ESLK+RIIISTKPPKEYL+A G ++ +EEAWG E+ N S+
Sbjct: 242 ESLKKRIIISTKPPKEYLEAKDVKEKENDSQKGKESADEEAWGKEVSNLKPSTVAVDESN 301
Query: 1020 KXXXXXXXXXXXXXXXTKAQQTEAPEYRRLIAIHAGKGKGGMRDWLKVDPNKVRRLSLSE 1199
K+QQ APEY+RLIAIHAGK KGG+ +WL+VDP+KVRRLSLSE
Sbjct: 302 LEEEENIDEEDLDEGDPKSQQNAAPEYKRLIAIHAGKVKGGLTEWLRVDPDKVRRLSLSE 361
Query: 1200 QELEKAIITYGKDIVRFTQRNLLRIYPKGIRLDSSNYNPLIGWSHGAQMVAFNMQGYGRS 1379
QELEKA++T+GK+IVRFTQRNLLR+YPKGIR DSSNYNP+IGW HGAQMVAFNMQGYGRS
Sbjct: 362 QELEKAVLTHGKEIVRFTQRNLLRVYPKGIRFDSSNYNPVIGWMHGAQMVAFNMQGYGRS 421
Query: 1380 LWVMQGMFKCNGGCGYVKKPEFLLRADAYGDVFDPKAXXXXXXXXXXXXYMGEGWYSDFK 1559
LW+M GMFK NGGCGYVKKP+FLL ++ VFDPKA YMGEGWY DF+
Sbjct: 422 LWIMHGMFKANGGCGYVKKPDFLLNNGSHDGVFDPKAKLPVKTTLKVTVYMGEGWYFDFR 481
Query: 1560 HTHFDTYSPPDFYARVGIAGVPADTIMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIE 1739
HTHFD YSPPDFYARVGIAGVPADTIMKKTKTLEDNW P WDE FEFPLTVPELALLR+E
Sbjct: 482 HTHFDAYSPPDFYARVGIAGVPADTIMKKTKTLEDNWTPNWDEHFEFPLTVPELALLRVE 541
Query: 1740 VHEYDMSEKDDFAGQTCLPISELRKGIRAVPLHTRKGTKYENVKLLMRFEFV 1895
VHEYDMSEKDDF GQTCLP+SELR+GIRAVPL +RKG KY +VKLLMRFEFV
Sbjct: 542 VHEYDMSEKDDFGGQTCLPVSELRRGIRAVPLFSRKGDKYPSVKLLMRFEFV 593
>ref|XP_003633437.1| PREDICTED: phosphoinositide phospholipase C 2 [Vitis vinifera]
Length = 580
Score = 874 bits (2258), Expect = 0.0
Identities = 429/583 (73%), Positives = 475/583 (81%), Gaps = 3/583 (0%)
Frame = +3
Query: 156 TKQTYXXXXXXXXXXXLTASVAPADIKELFTSYSENGVMFADHLQRFLVEVQKHETATVE 335
+KQTY LT S AP +IKELF YSENG+M DHLQRFL+EVQ+ E ATVE
Sbjct: 2 SKQTYRVCFCFRRRFRLTVSEAPQEIKELFDRYSENGMMSIDHLQRFLIEVQQEEKATVE 61
Query: 336 DAQAIIDSL---KHLSMFHRRGLHLEGFFRYLFGDANPPLNPDLGVHHDMTAPLSHYFIY 506
DAQAI+DSL KHL++FHR+ L+LE FF+YLFGD NPPL+ LGVHHDMTAPLSHYF+Y
Sbjct: 62 DAQAILDSLHEFKHLNIFHRKALNLEAFFKYLFGDINPPLSTALGVHHDMTAPLSHYFVY 121
Query: 507 TGHNSYLTGNQLSSDCSDVPIINALHSGVRVIELDIWPNSSNDDVDVLHGRTLTTPVALI 686
TGHNSYLTGNQLSSDCSDVPII+AL GVRVIELDIWPNS+ D+VDVLHGRTLTTPV LI
Sbjct: 122 TGHNSYLTGNQLSSDCSDVPIIHALQRGVRVIELDIWPNSTKDNVDVLHGRTLTTPVELI 181
Query: 687 KCLKSIKEHAFVKSEYPVVITLEDHLTPDLQAKVAEMITQTFEDILFCPGSECLKDFPSP 866
KCLKSIKEHAF SEYPVVITLEDHLTPDLQAKVAEM+TQTF DILF PG ECL +FPSP
Sbjct: 182 KCLKSIKEHAFSASEYPVVITLEDHLTPDLQAKVAEMVTQTFGDILFTPGPECLNEFPSP 241
Query: 867 ESLKRRIIISTKPPKEYLQAXXXXXXXXXXXXGMDATEEEAWGSEIKNFSSKXXXXXXXX 1046
ESLK+RIIISTKPPKEYL+A G ++ +EEAWG E S+
Sbjct: 242 ESLKKRIIISTKPPKEYLEAKDVKEKENDSQKGKESADEEAWGKE----SNLEEEENIDE 297
Query: 1047 XXXXXXXTKAQQTEAPEYRRLIAIHAGKGKGGMRDWLKVDPNKVRRLSLSEQELEKAIIT 1226
K+QQ APEY+RLIAIHAGK KGG+ +WL+VDP+KVRRLSLSEQELEKA++T
Sbjct: 298 EDLDEGDPKSQQNAAPEYKRLIAIHAGKVKGGLTEWLRVDPDKVRRLSLSEQELEKAVLT 357
Query: 1227 YGKDIVRFTQRNLLRIYPKGIRLDSSNYNPLIGWSHGAQMVAFNMQGYGRSLWVMQGMFK 1406
+GK+IVRFTQRNLLR+YPKGIR DSSNYNP+IGW HGAQMVAFNMQGYGRSLW+M GMFK
Sbjct: 358 HGKEIVRFTQRNLLRVYPKGIRFDSSNYNPVIGWMHGAQMVAFNMQGYGRSLWIMHGMFK 417
Query: 1407 CNGGCGYVKKPEFLLRADAYGDVFDPKAXXXXXXXXXXXXYMGEGWYSDFKHTHFDTYSP 1586
NGGCGYVKKP+FLL ++ VFDPKA YMGEGWY DF+HTHFD YSP
Sbjct: 418 ANGGCGYVKKPDFLLNNGSHDGVFDPKAKLPVKTTLKVTVYMGEGWYFDFRHTHFDAYSP 477
Query: 1587 PDFYARVGIAGVPADTIMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIEVHEYDMSEK 1766
PDFYARVGIAGVPADTIMKKTKTLEDNW P WDE FEFPLTVPELALLR+EVHEYDMSEK
Sbjct: 478 PDFYARVGIAGVPADTIMKKTKTLEDNWTPNWDEHFEFPLTVPELALLRVEVHEYDMSEK 537
Query: 1767 DDFAGQTCLPISELRKGIRAVPLHTRKGTKYENVKLLMRFEFV 1895
DDF GQTCLP+SELR+GIRAVPL +RKG KY +VKLLMRFEFV
Sbjct: 538 DDFGGQTCLPVSELRRGIRAVPLFSRKGDKYPSVKLLMRFEFV 580
>ref|XP_002270230.1| PREDICTED: phosphoinositide phospholipase C 2 isoform 2 [Vitis
vinifera]
Length = 592
Score = 866 bits (2238), Expect = 0.0
Identities = 428/592 (72%), Positives = 475/592 (80%), Gaps = 12/592 (2%)
Frame = +3
Query: 156 TKQTYXXXXXXXXXXXLTASVAPADIKELFTSYSENGVMFADHLQRFLVEVQKHETATVE 335
+KQTY LT S AP +IKELF YSENG+M DHLQRFL+EVQ+ E ATVE
Sbjct: 2 SKQTYRVCFCFRRRFRLTVSEAPQEIKELFDRYSENGMMSIDHLQRFLIEVQQEEKATVE 61
Query: 336 DAQAIIDSL---KHLSMFHRRGLHLEGFFRYLFGDANPPLNPDLGVHHDMTAPLSHYFIY 506
DAQAI+DSL KHL++FHR+ L+LE FF+YLFGD NPPL+ VHHDMTAPLSHYF+Y
Sbjct: 62 DAQAILDSLHEFKHLNIFHRKALNLEAFFKYLFGDINPPLSL-FQVHHDMTAPLSHYFVY 120
Query: 507 TGHNSYLTGNQLSSDCSDVPIINALHSGVRVIELDIWPNSSNDDVDVLHGRTLTTPVALI 686
TGHNSYLTGNQLSSDCSDVPII+AL GVRVIELDIWPNS+ D+VDVLHGRTLTTPV LI
Sbjct: 121 TGHNSYLTGNQLSSDCSDVPIIHALQRGVRVIELDIWPNSTKDNVDVLHGRTLTTPVELI 180
Query: 687 KCLKSIKEHAFVKSEYPVVITLEDHLTPDLQAKVAEMITQTFEDILFCPGSECLKDFPSP 866
KCLKSIKEHAF SEYPVVITLEDHLTPDLQAKVAEM+TQTF DILF PG ECL +FPSP
Sbjct: 181 KCLKSIKEHAFSASEYPVVITLEDHLTPDLQAKVAEMVTQTFGDILFTPGPECLNEFPSP 240
Query: 867 ESLKRRIIISTKPPKEYLQAXXXXXXXXXXXXGMDATEEEAWGSEIKNF---------SS 1019
ESLK+RIIISTKPPKEYL+A G ++ +EEAWG E+ N S+
Sbjct: 241 ESLKKRIIISTKPPKEYLEAKDVKEKENDSQKGKESADEEAWGKEVSNLKPSTVAVDESN 300
Query: 1020 KXXXXXXXXXXXXXXXTKAQQTEAPEYRRLIAIHAGKGKGGMRDWLKVDPNKVRRLSLSE 1199
K+QQ APEY+RLIAIHAGK KGG+ +WL+VDP+KVRRLSLSE
Sbjct: 301 LEEEENIDEEDLDEGDPKSQQNAAPEYKRLIAIHAGKVKGGLTEWLRVDPDKVRRLSLSE 360
Query: 1200 QELEKAIITYGKDIVRFTQRNLLRIYPKGIRLDSSNYNPLIGWSHGAQMVAFNMQGYGRS 1379
QELEKA++T+GK+IVRFTQRNLLR+YPKGIR DSSNYNP+IGW HGAQMVAFNMQGYGRS
Sbjct: 361 QELEKAVLTHGKEIVRFTQRNLLRVYPKGIRFDSSNYNPVIGWMHGAQMVAFNMQGYGRS 420
Query: 1380 LWVMQGMFKCNGGCGYVKKPEFLLRADAYGDVFDPKAXXXXXXXXXXXXYMGEGWYSDFK 1559
LW+M GMFK NGGCGYVKKP+FLL ++ VFDPKA YMGEGWY DF+
Sbjct: 421 LWIMHGMFKANGGCGYVKKPDFLLNNGSHDGVFDPKAKLPVKTTLKVTVYMGEGWYFDFR 480
Query: 1560 HTHFDTYSPPDFYARVGIAGVPADTIMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIE 1739
HTHFD YSPPDFYARVGIAGVPADTIMKKTKTLEDNW P WDE FEFPLTVPELALLR+E
Sbjct: 481 HTHFDAYSPPDFYARVGIAGVPADTIMKKTKTLEDNWTPNWDEHFEFPLTVPELALLRVE 540
Query: 1740 VHEYDMSEKDDFAGQTCLPISELRKGIRAVPLHTRKGTKYENVKLLMRFEFV 1895
VHEYDMSEKDDF GQTCLP+SELR+GIRAVPL +RKG KY +VKLLMRFEFV
Sbjct: 541 VHEYDMSEKDDFGGQTCLPVSELRRGIRAVPLFSRKGDKYPSVKLLMRFEFV 592
>ref|XP_002311223.1| predicted protein [Populus trichocarpa] gi|222851043|gb|EEE88590.1|
predicted protein [Populus trichocarpa]
Length = 590
Score = 845 bits (2184), Expect = 0.0
Identities = 418/591 (70%), Positives = 471/591 (79%), Gaps = 11/591 (1%)
Frame = +3
Query: 156 TKQTYXXXXXXXXXXXLTASVAPADIKELFTSYSENGVMFADHLQRFLVEVQKHETATVE 335
+KQTY L + P +IK LF YSENG+M ADH+ RFL+EVQK E AT E
Sbjct: 2 SKQTYRVCFCFRRRFKLAVAEVPDEIKALFDLYSENGIMTADHIHRFLIEVQKQEKATFE 61
Query: 336 DAQAIIDSLKHLSMFHRRGLHLEGFFRYLFGDANPPLNPDLGVHHDMTAPLSHYFIYTGH 515
+AQ+I++SLKHLS+FHR+GLHLE FF+YLFGD NPPL+ LGVHHDMTAPLSHYFIYTGH
Sbjct: 62 EAQSIVESLKHLSLFHRKGLHLEAFFKYLFGDTNPPLDLKLGVHHDMTAPLSHYFIYTGH 121
Query: 516 NSYLTGNQLSSDCSDVPIINALHSGVRVIELDIWPNSSNDDVDVLHGRTLTTPVALIKCL 695
NSYLTGNQLSS+CSDVPIINAL GVRVIELDIWPNS NDDV+VLHGRTLTTPV LIKCL
Sbjct: 122 NSYLTGNQLSSECSDVPIINALKKGVRVIELDIWPNSDNDDVEVLHGRTLTTPVQLIKCL 181
Query: 696 KSIKEHAFVKSEYPVVITLEDHLTPDLQAKVAEMITQTFEDILFCPGSECLKDFPSPESL 875
+SI EHAF SE+PVVITLEDHLTPDLQAKVA+M+TQTF DILF PGSE LK+FPSP+SL
Sbjct: 182 RSINEHAFTASEFPVVITLEDHLTPDLQAKVAQMVTQTFGDILFTPGSERLKEFPSPDSL 241
Query: 876 KRRIIISTKPPKEYLQAXXXXXXXXXXXXGMDATEEEAWGSEI-----------KNFSSK 1022
KRRIIISTKPPKEYL+A G A++EEAWG EI KN S K
Sbjct: 242 KRRIIISTKPPKEYLEAKEIKDKESHYQKGNAASDEEAWGKEIPILNGHILADDKNESDK 301
Query: 1023 XXXXXXXXXXXXXXXTKAQQTEAPEYRRLIAIHAGKGKGGMRDWLKVDPNKVRRLSLSEQ 1202
K Q APEY+RLIAIHAGK KGG+ + LKVDP+KVRRLSLSEQ
Sbjct: 302 DDDDAEEDLDEGDH--KLQHDIAPEYKRLIAIHAGKPKGGLDECLKVDPDKVRRLSLSEQ 359
Query: 1203 ELEKAIITYGKDIVRFTQRNLLRIYPKGIRLDSSNYNPLIGWSHGAQMVAFNMQGYGRSL 1382
+LEKA T+GK+IVRFTQRN+LR+YPKG R++SSNYNPLIGW HGAQMVAFNMQGYGRSL
Sbjct: 360 QLEKAAETHGKEIVRFTQRNILRVYPKGTRVNSSNYNPLIGWMHGAQMVAFNMQGYGRSL 419
Query: 1383 WVMQGMFKCNGGCGYVKKPEFLLRADAYGDVFDPKAXXXXXXXXXXXXYMGEGWYSDFKH 1562
W+MQGMF+ NGGCG+VKKP FLL++ +G+VFD KA YMGEGW+ DF+H
Sbjct: 420 WLMQGMFRANGGCGFVKKPSFLLKSGPHGEVFDTKAKLPMQKTLKVKIYMGEGWFYDFQH 479
Query: 1563 THFDTYSPPDFYARVGIAGVPADTIMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIEV 1742
THFD YSPPDFYARVGIAGVPADT+MKKTKTLED+WIP W+E FEFPLTVPELALLRIEV
Sbjct: 480 THFDAYSPPDFYARVGIAGVPADTVMKKTKTLEDSWIPFWNEEFEFPLTVPELALLRIEV 539
Query: 1743 HEYDMSEKDDFAGQTCLPISELRKGIRAVPLHTRKGTKYENVKLLMRFEFV 1895
HEYDMSEKDDF GQTCLP+ ELR+GIRAVPLH RKG +Y+ VKLL+R EFV
Sbjct: 540 HEYDMSEKDDFGGQTCLPVWELREGIRAVPLHDRKGERYKCVKLLVRLEFV 590
>ref|XP_002316212.1| predicted protein [Populus trichocarpa] gi|222865252|gb|EEF02383.1|
predicted protein [Populus trichocarpa]
Length = 587
Score = 838 bits (2166), Expect = 0.0
Identities = 410/586 (69%), Positives = 463/586 (79%), Gaps = 6/586 (1%)
Frame = +3
Query: 156 TKQTYXXXXXXXXXXXLTASVAPADIKELFTSYSENGVMFADHLQRFLVEVQKHETATVE 335
+KQTY L + AP +I+ LF YS+NG+M +HL RFL+EVQK E AT+E
Sbjct: 2 SKQTYRVCLCFSRRFKLAVAEAPEEIRALFNQYSDNGIMTDNHLHRFLIEVQKQEKATLE 61
Query: 336 DAQAIIDSLKHLSMFHRRGLHLEGFFRYLFGDANPPLNPDLGVHHDMTAPLSHYFIYTGH 515
+AQAII+SLKHL++FHR+GL+LE FF+YLFGD NPPL+ LG HHDMTAP+SHYFIYTGH
Sbjct: 62 EAQAIIESLKHLAIFHRKGLNLEAFFKYLFGDNNPPLDLKLGAHHDMTAPISHYFIYTGH 121
Query: 516 NSYLTGNQLSSDCSDVPIINALHSGVRVIELDIWPNSSNDDVDVLHGRTLTTPVALIKCL 695
NSYLTGNQLSSDCSDVPIINAL GVRVIELDIWPNS DDV+VLHGRTLTTPV LIKCL
Sbjct: 122 NSYLTGNQLSSDCSDVPIINALKKGVRVIELDIWPNSDKDDVEVLHGRTLTTPVQLIKCL 181
Query: 696 KSIKEHAFVKSEYPVVITLEDHLTPDLQAKVAEMITQTFEDILFCPGSECLKDFPSPESL 875
+SIKEHAF SE+PVVITLEDHLTPDLQ+KV++M+TQTF D LF PGSECLK+FPSPESL
Sbjct: 182 RSIKEHAFTASEFPVVITLEDHLTPDLQSKVSKMVTQTFGDTLFSPGSECLKEFPSPESL 241
Query: 876 KRRIIISTKPPKEYLQAXXXXXXXXXXXXGMDATEEEAWGSEIKNFSSKXXXXXXXXXXX 1055
KRRIIISTKPPKEYL+A G A +EEAWG EI N
Sbjct: 242 KRRIIISTKPPKEYLEAKEIKDKESDSQKGNAAPDEEAWGKEILNLKGADDKNELDEDDN 301
Query: 1056 XXXXT------KAQQTEAPEYRRLIAIHAGKGKGGMRDWLKVDPNKVRRLSLSEQELEKA 1217
K APEY+RLIAI AGK KGG+ + LKVDP+K RRLSLSEQ+LE A
Sbjct: 302 DAEEDPGEGDHKLPHDIAPEYKRLIAIPAGKPKGGLEECLKVDPDKARRLSLSEQQLENA 361
Query: 1218 IITYGKDIVRFTQRNLLRIYPKGIRLDSSNYNPLIGWSHGAQMVAFNMQGYGRSLWVMQG 1397
T+GK+IVRFTQRN+LR+YPKGIR++SSNYNPLIGW HGAQMVAFNMQGYGRSLW+MQG
Sbjct: 362 AETHGKEIVRFTQRNILRVYPKGIRVNSSNYNPLIGWMHGAQMVAFNMQGYGRSLWMMQG 421
Query: 1398 MFKCNGGCGYVKKPEFLLRADAYGDVFDPKAXXXXXXXXXXXXYMGEGWYSDFKHTHFDT 1577
MF+ NGGCG+VKKP+FLL++ +G+VFDPKA YMGEGWY DF HTHFD
Sbjct: 422 MFRANGGCGFVKKPDFLLKSGPHGEVFDPKAKLPVQKTLKVKIYMGEGWYYDFHHTHFDA 481
Query: 1578 YSPPDFYARVGIAGVPADTIMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIEVHEYDM 1757
YSPPDFY RVGIAGVPADT MKKT+TLEDNWIP WDE FEFPLTVP+LALLRIEVHEYDM
Sbjct: 482 YSPPDFYVRVGIAGVPADTGMKKTRTLEDNWIPVWDEGFEFPLTVPDLALLRIEVHEYDM 541
Query: 1758 SEKDDFAGQTCLPISELRKGIRAVPLHTRKGTKYENVKLLMRFEFV 1895
SEKDDF GQTCLP+ ELR+GIRAVPLH RKG KY +VKLL+R EFV
Sbjct: 542 SEKDDFGGQTCLPVRELREGIRAVPLHDRKGEKYNSVKLLVRLEFV 587