BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Scutellaria23_contig00009361
         (2658 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003554958.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   934   0.0  
ref|XP_003554957.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   933   0.0  
ref|XP_002281380.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   929   0.0  
ref|XP_002306060.1| predicted protein [Populus trichocarpa] gi|2...   927   0.0  
ref|XP_003544005.1| PREDICTED: probable N(2),N(2)-dimethylguanos...   926   0.0  

>ref|XP_003554958.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like isoform 2 [Glycine max]
          Length = 645

 Score =  934 bits (2413), Expect = 0.0
 Identities = 469/582 (80%), Positives = 510/582 (87%), Gaps = 9/582 (1%)
 Frame = +3

Query: 111  DASEATPFDINDFTVIKEGEAEILMHVKNEVFYNKAQVNNRDMSIAVLRTFISKRKQEHE 290
            D       D++D+ +IKEGEAEILMH KNEVFYNK QVNNRD+SIAVLRTFISKRKQEHE
Sbjct: 61   DIGRTMSSDLSDYKIIKEGEAEILMHAKNEVFYNKTQVNNRDISIAVLRTFISKRKQEHE 120

Query: 291  ANLSRKMKNGPKVAEE----PVVESELPTDNVERD--SNGE--VSEEICQDEPCSNSADL 446
            A LS++ K   KV+E      +V+ E+P      D  SNGE  V EEI  +E CS   + 
Sbjct: 121  AYLSKRTKGAQKVSENGDSSELVKEEVPDSTAPEDHKSNGECEVEEEISPEEQCSTMEED 180

Query: 447  AAN-HEGKGRGELKPPCVLEALSASGLRALRYAREVDGIGQVVALDNDKASVESCRRNIK 623
            + N  E   + ELKPP VLEALSASGLR+LRYAREV+GIGQV+A+DNDKASVE+CRRNIK
Sbjct: 181  SVNVTEECRKRELKPPRVLEALSASGLRSLRYAREVEGIGQVIAVDNDKASVEACRRNIK 240

Query: 624  FNGSVACSKVESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSIVDGGILMCTA 803
            FNGSVA SKVESHLADARVYMLTH KEFDVVDLDPYGSPS+FLDSAVQS+VDGG+LMCTA
Sbjct: 241  FNGSVAASKVESHLADARVYMLTHAKEFDVVDLDPYGSPSMFLDSAVQSVVDGGMLMCTA 300

Query: 804  TDMAVLCGSNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMD 983
            TDMAVLCG NGEVCYSKYGSYPLRGKYCHEMALRI+LACIESHANRYKRYIVPVLSVQMD
Sbjct: 301  TDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIESHANRYKRYIVPVLSVQMD 360

Query: 984  FYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTVSKNTSVRYLPGFGPLV 1163
            FY+RVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRT+SKNTSVRYLPGFGP+V
Sbjct: 361  FYLRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTISKNTSVRYLPGFGPVV 420

Query: 1164 GQECSDCGKKYNMGGPIWSAPIHDMEWVTSILEDVRSMKDRYPAFGRISAVLTTISEELP 1343
             QEC DCGKK+NMGGPIWSAPIHD EWV +IL DV+SMKDRYPA+ RISAVLTTISEELP
Sbjct: 421  PQECRDCGKKFNMGGPIWSAPIHDQEWVAAILADVKSMKDRYPAYDRISAVLTTISEELP 480

Query: 1344 DVPLFLSLHNLCATLKCTSPSAVVFRSAVINAGYRISGSHVNPLGLKTDAPMDVIWDIMR 1523
            DVPLFLSLHNLCATLKCTSPSA++FRSAVINAGYRISG+HVNPLGLK+DAPMDVIWDIMR
Sbjct: 481  DVPLFLSLHNLCATLKCTSPSAIIFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMR 540

Query: 1524 SWVKNHPVKTQPSDQAGSVILAKEPELQVNFTRAVASLSRAQAKKVARFLPNPERHWGPK 1703
             WVKNHPVK QP+DQ GS+ILAKEP LQ NF RAVASLS+AQAKKVARFLPNPERHWGPK
Sbjct: 541  CWVKNHPVKPQPADQPGSIILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPK 600

Query: 1704 LRAGRQITSKHVSLLGVEAIKGILDSENDEGHEPPSKRKKIE 1829
            LRAGRQITSKHVSLLG EAI G L+ E+DE  EP SK+ K E
Sbjct: 601  LRAGRQITSKHVSLLGPEAINGALNQEDDE--EPKSKKPKTE 640


>ref|XP_003554957.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like isoform 1 [Glycine max]
          Length = 638

 Score =  933 bits (2412), Expect = 0.0
 Identities = 468/581 (80%), Positives = 508/581 (87%), Gaps = 8/581 (1%)
 Frame = +3

Query: 111  DASEATPFDINDFTVIKEGEAEILMHVKNEVFYNKAQVNNRDMSIAVLRTFISKRKQEHE 290
            D       D++D+ +IKEGEAEILMH KNEVFYNK QVNNRD+SIAVLRTFISKRKQEHE
Sbjct: 61   DIGRTMSSDLSDYKIIKEGEAEILMHAKNEVFYNKTQVNNRDISIAVLRTFISKRKQEHE 120

Query: 291  ANLSRKMKNGPKVAEE----PVVESELPTDNVERD--SNGE--VSEEICQDEPCSNSADL 446
            A LS++ K   KV+E      +V+ E+P      D  SNGE  V EEI  +E CS   + 
Sbjct: 121  AYLSKRTKGAQKVSENGDSSELVKEEVPDSTAPEDHKSNGECEVEEEISPEEQCSTMEED 180

Query: 447  AANHEGKGRGELKPPCVLEALSASGLRALRYAREVDGIGQVVALDNDKASVESCRRNIKF 626
            + N E      LKPP VLEALSASGLR+LRYAREV+GIGQV+A+DNDKASVE+CRRNIKF
Sbjct: 181  SVNRE------LKPPRVLEALSASGLRSLRYAREVEGIGQVIAVDNDKASVEACRRNIKF 234

Query: 627  NGSVACSKVESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSIVDGGILMCTAT 806
            NGSVA SKVESHLADARVYMLTH KEFDVVDLDPYGSPS+FLDSAVQS+VDGG+LMCTAT
Sbjct: 235  NGSVAASKVESHLADARVYMLTHAKEFDVVDLDPYGSPSMFLDSAVQSVVDGGMLMCTAT 294

Query: 807  DMAVLCGSNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDF 986
            DMAVLCG NGEVCYSKYGSYPLRGKYCHEMALRI+LACIESHANRYKRYIVPVLSVQMDF
Sbjct: 295  DMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIESHANRYKRYIVPVLSVQMDF 354

Query: 987  YVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTVSKNTSVRYLPGFGPLVG 1166
            Y+RVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRT+SKNTSVRYLPGFGP+V 
Sbjct: 355  YLRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTISKNTSVRYLPGFGPVVP 414

Query: 1167 QECSDCGKKYNMGGPIWSAPIHDMEWVTSILEDVRSMKDRYPAFGRISAVLTTISEELPD 1346
            QEC DCGKK+NMGGPIWSAPIHD EWV +IL DV+SMKDRYPA+ RISAVLTTISEELPD
Sbjct: 415  QECRDCGKKFNMGGPIWSAPIHDQEWVAAILADVKSMKDRYPAYDRISAVLTTISEELPD 474

Query: 1347 VPLFLSLHNLCATLKCTSPSAVVFRSAVINAGYRISGSHVNPLGLKTDAPMDVIWDIMRS 1526
            VPLFLSLHNLCATLKCTSPSA++FRSAVINAGYRISG+HVNPLGLK+DAPMDVIWDIMR 
Sbjct: 475  VPLFLSLHNLCATLKCTSPSAIIFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRC 534

Query: 1527 WVKNHPVKTQPSDQAGSVILAKEPELQVNFTRAVASLSRAQAKKVARFLPNPERHWGPKL 1706
            WVKNHPVK QP+DQ GS+ILAKEP LQ NF RAVASLS+AQAKKVARFLPNPERHWGPKL
Sbjct: 535  WVKNHPVKPQPADQPGSIILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKL 594

Query: 1707 RAGRQITSKHVSLLGVEAIKGILDSENDEGHEPPSKRKKIE 1829
            RAGRQITSKHVSLLG EAI G L+ E+DE  EP SK+ K E
Sbjct: 595  RAGRQITSKHVSLLGPEAINGALNQEDDE--EPKSKKPKTE 633


>ref|XP_002281380.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 2 [Vitis vinifera]
            gi|147815608|emb|CAN65976.1| hypothetical protein
            VITISV_036159 [Vitis vinifera]
            gi|298204459|emb|CBI16939.3| unnamed protein product
            [Vitis vinifera]
          Length = 579

 Score =  929 bits (2402), Expect = 0.0
 Identities = 468/572 (81%), Positives = 504/572 (88%), Gaps = 7/572 (1%)
 Frame = +3

Query: 135  DINDFTVIKEGEAEILMHVKNEVFYNKAQVNNRDMSIAVLRTFISKRKQEHEANLSRKMK 314
            D++D+++IKEGEAEILMH KNEVFYNK QVNNRD+SIAVLRTFISKRKQEHEA LS++ K
Sbjct: 4    DLSDYSIIKEGEAEILMHAKNEVFYNKTQVNNRDISIAVLRTFISKRKQEHEAKLSKRTK 63

Query: 315  NGPKVAEEPVVE---SELPT--DNVERDSNGE--VSEEICQDEPCSNSADLAANHEGKGR 473
               KV+EE   E    E PT  D  E  SNGE  V EE   DEP   S +     E KG+
Sbjct: 64   AAKKVSEEDASEPLAKEAPTESDMHEEKSNGECAVPEETSLDEPHCISEEPVGIMEEKGQ 123

Query: 474  GELKPPCVLEALSASGLRALRYAREVDGIGQVVALDNDKASVESCRRNIKFNGSVACSKV 653
            G+LKPP VLEALSASGLRALRYAREV+G+GQVVALDNDK SVE+CRRNIKFNGSVA SKV
Sbjct: 124  GQLKPPRVLEALSASGLRALRYAREVEGVGQVVALDNDKVSVEACRRNIKFNGSVASSKV 183

Query: 654  ESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSIVDGGILMCTATDMAVLCGSN 833
            +S LADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQ + DGG+LMCTATDMAVLCG N
Sbjct: 184  DSQLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQCVADGGMLMCTATDMAVLCGGN 243

Query: 834  GEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIY 1013
            GE CYSKYGSYPLRGKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVFVRIY
Sbjct: 244  GEACYSKYGSYPLRGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVFVRIY 303

Query: 1014 TSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTVSKNTSVRYLPGFGPLVGQECSDCGKK 1193
            TSASAMK+TPLKLSYVYQCTGCDSFHLQPIGRTVSKN SVRYLPGFGP+V QECSDCGKK
Sbjct: 304  TSASAMKSTPLKLSYVYQCTGCDSFHLQPIGRTVSKNNSVRYLPGFGPVVPQECSDCGKK 363

Query: 1194 YNMGGPIWSAPIHDMEWVTSILEDVRSMKDRYPAFGRISAVLTTISEELPDVPLFLSLHN 1373
            +NMGGPIWSAPIHD EWVTSIL DV+SMKDRYPA+ RISAVLTTISEELPDVPLFLSLHN
Sbjct: 364  FNMGGPIWSAPIHDQEWVTSILADVKSMKDRYPAYNRISAVLTTISEELPDVPLFLSLHN 423

Query: 1374 LCATLKCTSPSAVVFRSAVINAGYRISGSHVNPLGLKTDAPMDVIWDIMRSWVKNHPVKT 1553
            LCATLKCTSPSAV+FRSAV+NAGYRISG+HVNPLG+K+DAPMDVIWDIMR WVKNHPVK 
Sbjct: 424  LCATLKCTSPSAVIFRSAVLNAGYRISGTHVNPLGMKSDAPMDVIWDIMRCWVKNHPVKA 483

Query: 1554 QPSDQAGSVILAKEPELQVNFTRAVASLSRAQAKKVARFLPNPERHWGPKLRAGRQITSK 1733
            Q  DQ+GSVILAKEP LQ NF RAVASLS+AQ KKVARFLPNPERHWGPKLRAGRQITSK
Sbjct: 484  QQPDQSGSVILAKEPVLQANFARAVASLSKAQTKKVARFLPNPERHWGPKLRAGRQITSK 543

Query: 1734 HVSLLGVEAIKGILDSENDEGHEPPSKRKKIE 1829
            HVSLLG EA+ G ++ + DE  EP SKR+K +
Sbjct: 544  HVSLLGPEAVNGSMNHQEDE--EPNSKRQKTD 573


>ref|XP_002306060.1| predicted protein [Populus trichocarpa] gi|222849024|gb|EEE86571.1|
            predicted protein [Populus trichocarpa]
          Length = 582

 Score =  927 bits (2396), Expect = 0.0
 Identities = 462/577 (80%), Positives = 510/577 (88%), Gaps = 7/577 (1%)
 Frame = +3

Query: 135  DINDFTVIKEGEAEILMHVKNEVFYNKAQVNNRDMSIAVLRTFISKRKQEHEANLSRKMK 314
            D+ D+T+IKEGEA+ILM+VKN+VF+NKAQVNNRD+SIAVLRTFISKR++EHEA LS++ K
Sbjct: 4    DLQDYTIIKEGEADILMNVKNQVFFNKAQVNNRDLSIAVLRTFISKRQEEHEAMLSKRTK 63

Query: 315  NGPKVAEE----PVVESELP---TDNVERDSNGEVSEEICQDEPCSNSADLAANHEGKGR 473
            +  K +E+    P VE        DN + +   EV EEI Q+EPCS S +   N EG   
Sbjct: 64   SAKKASEKDASGPAVEEASTGSALDNEKSNGECEVHEEISQNEPCSISEESVKNTEGNHH 123

Query: 474  GELKPPCVLEALSASGLRALRYAREVDGIGQVVALDNDKASVESCRRNIKFNGSVACSKV 653
            GELKPP VLEALSASGLRALRYAREV+GIG+VVALDNDKASVE+C++NIKFNGSVA SKV
Sbjct: 124  GELKPPKVLEALSASGLRALRYAREVEGIGKVVALDNDKASVEACQKNIKFNGSVAISKV 183

Query: 654  ESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSIVDGGILMCTATDMAVLCGSN 833
            ESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQS+VDGG+LMCTATDMAVLCG N
Sbjct: 184  ESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSVVDGGMLMCTATDMAVLCGGN 243

Query: 834  GEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRIY 1013
            GEVCYSKYGSYPLRGKYCHEMALRILLA IESHANRYKRYIVPVLSVQM+FYVRVFVR+Y
Sbjct: 244  GEVCYSKYGSYPLRGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMNFYVRVFVRVY 303

Query: 1014 TSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTVSKNTSVRYLPGFGPLVGQECSDCGKK 1193
            TSASAMKNTPLKLSYVYQC GCDS+HLQPIGRT+SK +SVR+LPGFGP+V QECSDCGKK
Sbjct: 304  TSASAMKNTPLKLSYVYQCIGCDSYHLQPIGRTMSKGSSVRHLPGFGPVVPQECSDCGKK 363

Query: 1194 YNMGGPIWSAPIHDMEWVTSILEDVRSMKDRYPAFGRISAVLTTISEELPDVPLFLSLHN 1373
            YNMGGPIWSAPIHD EWVTS+L+ V+SMKDRYPA+ RISAVLTTISEELPDVPLFLSLHN
Sbjct: 364  YNMGGPIWSAPIHDQEWVTSVLDGVKSMKDRYPAYDRISAVLTTISEELPDVPLFLSLHN 423

Query: 1374 LCATLKCTSPSAVVFRSAVINAGYRISGSHVNPLGLKTDAPMDVIWDIMRSWVKNHPVKT 1553
            LCATLKCTSPSAV+FRSAVINAGYRISG+HVNPLGLK+DAPMDVIWDIMR WVKNHPVK 
Sbjct: 424  LCATLKCTSPSAVIFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKA 483

Query: 1554 QPSDQAGSVILAKEPELQVNFTRAVASLSRAQAKKVARFLPNPERHWGPKLRAGRQITSK 1733
            Q  DQ GS+ILAKEP LQ NF RAVASLS+AQAKKVARFLPNPERHWGPKLRAGRQIT+K
Sbjct: 484  QAPDQPGSIILAKEPVLQANFARAVASLSKAQAKKVARFLPNPERHWGPKLRAGRQITTK 543

Query: 1734 HVSLLGVEAIKGILDSENDEGHEPPSKRKKIEIEDDP 1844
            H+SLLG EA+ G L+ EN E  E  +KR K    +DP
Sbjct: 544  HISLLGPEAVNGHLNHENSE--ETKAKRPKTGDTNDP 578


>ref|XP_003544005.1| PREDICTED: probable N(2),N(2)-dimethylguanosine tRNA
            methyltransferase 1-like [Glycine max]
          Length = 582

 Score =  926 bits (2392), Expect = 0.0
 Identities = 464/575 (80%), Positives = 502/575 (87%), Gaps = 22/575 (3%)
 Frame = +3

Query: 135  DINDFTVIKEGEAEILMHVKNEVFYNKAQVNNRDMSIAVLRTFISKRKQEHEANLSRKMK 314
            D++D+ +IKEGEAEILMH KNEVFYNK QVNNRD+SIAVLRTFISKRKQEHEA LS++ K
Sbjct: 4    DLSDYKIIKEGEAEILMHAKNEVFYNKTQVNNRDISIAVLRTFISKRKQEHEAYLSKRTK 63

Query: 315  NGPKVAEE----PVVESELPTDNVER--DSNGE--VSEEICQDEPCSN----------SA 440
               K +E      +V+ E+P    +    SNGE  V EEI  +E CS             
Sbjct: 64   GAQKASENGDSSELVKEEVPDTTAQEYHKSNGECEVEEEISPEERCSTVEEGSVKVTEEC 123

Query: 441  DLAANH----EGKGRGELKPPCVLEALSASGLRALRYAREVDGIGQVVALDNDKASVESC 608
              A  H    EGKG+ ELKPP VLEALSASGLR+LRYAREV+GIGQV+A+DNDKASVE+C
Sbjct: 124  STAEGHTNLSEGKGKRELKPPRVLEALSASGLRSLRYAREVEGIGQVIAVDNDKASVEAC 183

Query: 609  RRNIKFNGSVACSKVESHLADARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSIVDGGI 788
            RRNIKFNGSVA SKVESHL DARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQS VDGG+
Sbjct: 184  RRNIKFNGSVAASKVESHLDDARVYMLTHPKEFDVVDLDPYGSPSVFLDSAVQSTVDGGM 243

Query: 789  LMCTATDMAVLCGSNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVL 968
            LMCTATDMAVLCG NGEVCYSKYGSYPLRGKYCHEMALRI+LACIESHANRYKRYIVPVL
Sbjct: 244  LMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIESHANRYKRYIVPVL 303

Query: 969  SVQMDFYVRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTVSKNTSVRYLPG 1148
            SVQMDFY+RVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRT+SKNTSVRYLPG
Sbjct: 304  SVQMDFYLRVFVRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQPIGRTISKNTSVRYLPG 363

Query: 1149 FGPLVGQECSDCGKKYNMGGPIWSAPIHDMEWVTSILEDVRSMKDRYPAFGRISAVLTTI 1328
            FGP+V QECSDCGKK+NMGGPIWSAPIHD EWV +IL DV+ MKDRYPA+ RISAVLTTI
Sbjct: 364  FGPVVPQECSDCGKKFNMGGPIWSAPIHDQEWVAAILADVKYMKDRYPAYDRISAVLTTI 423

Query: 1329 SEELPDVPLFLSLHNLCATLKCTSPSAVVFRSAVINAGYRISGSHVNPLGLKTDAPMDVI 1508
            SEELPDVPLFLSLHNLCATLKCTSPSA++FRSAVINAGYRISG+HVNPLGLK+DAPMDVI
Sbjct: 424  SEELPDVPLFLSLHNLCATLKCTSPSAIIFRSAVINAGYRISGTHVNPLGLKSDAPMDVI 483

Query: 1509 WDIMRSWVKNHPVKTQPSDQAGSVILAKEPELQVNFTRAVASLSRAQAKKVARFLPNPER 1688
            WDIMR WVKNHPVK QP+DQ GS+ILAKEP LQ NF RAVASLS+AQAKKVARFLPNPER
Sbjct: 484  WDIMRCWVKNHPVKPQPADQPGSIILAKEPALQANFARAVASLSKAQAKKVARFLPNPER 543

Query: 1689 HWGPKLRAGRQITSKHVSLLGVEAIKGILDSENDE 1793
            HWGPKLRAGRQITSKHVSLLG +AI G L+ E+DE
Sbjct: 544  HWGPKLRAGRQITSKHVSLLGPDAINGALNQEDDE 578