BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Scutellaria23_contig00007506
         (2278 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADN33838.1| pre-mRNA splicing factor [Cucumis melo subsp. melo]    928   0.0  
ref|XP_002312609.1| predicted protein [Populus trichocarpa] gi|2...   928   0.0  
ref|XP_004155354.1| PREDICTED: LOW QUALITY PROTEIN: pre-mRNA-pro...   928   0.0  
ref|XP_004135243.1| PREDICTED: pre-mRNA-processing factor 17-lik...   928   0.0  
ref|XP_002262666.1| PREDICTED: pre-mRNA-processing factor 17-lik...   926   0.0  

>gb|ADN33838.1| pre-mRNA splicing factor [Cucumis melo subsp. melo]
          Length = 581

 Score =  928 bits (2399), Expect = 0.0
 Identities = 445/596 (74%), Positives = 487/596 (81%), Gaps = 3/596 (0%)
 Frame = +3

Query: 24   MDLLRSYKESEIDDDEKVDNVXXXXXXXXXXXXXXXXXXXXXXLPAKSAAPKVDDTMLSL 203
            MDLL+SY +   DD                             +P+K++APKVDDTML+L
Sbjct: 1    MDLLQSYTDQNDDDGSPKQ------------LTSSPEASPPRLIPSKTSAPKVDDTMLAL 48

Query: 204  TVAGQAHSLTHKPIDPTQHLVSFNPTYDQLWAPIVGPSHPYAKDGLAQGLRNHKLGFVEN 383
            TVA    +L+ KPIDPTQHLV+FNPTYDQLWAPI GPSHPYAKDG+AQG+RNHKLGFVEN
Sbjct: 49   TVANANQTLS-KPIDPTQHLVAFNPTYDQLWAPIYGPSHPYAKDGIAQGMRNHKLGFVEN 107

Query: 384  ANIESFAFDEQYNTFYKFGYAADPSQ---NTYVGDVEGFEKNEGISVYNIPQHXXXXXXX 554
            A+IE F FDEQYNTF+K+GYAADPS    N Y+GD+E  +KN+ ISVYNIPQH       
Sbjct: 108  ASIEPFVFDEQYNTFHKYGYAADPSASAGNNYIGDMEALQKNDAISVYNIPQHEQKKRKI 167

Query: 555  XXXXXXXXXXXGDAEVDKDELGNPATETWLMKNRKSPWAGKREWVQGELTXXXXXXXXXX 734
                        + EV+  E+ NPA++ WLMKNRKSPW+GK+E +Q ELT          
Sbjct: 168  EKKKEMSENEDMEEEVNPAEVDNPASDVWLMKNRKSPWSGKKEGLQTELTEEQKKYAEEY 227

Query: 735  XXXXXXXXXXXXXXXXXXXDKSTFHGKEEKDYQGRSWIAPPKDAKASNDHCYIPKRLVHT 914
                               DKSTFHGKEE+DYQGRSWIAPPKDAKA+NDHCYIPKRLVHT
Sbjct: 228  AKKKGEEKGGEKGEVTA--DKSTFHGKEERDYQGRSWIAPPKDAKATNDHCYIPKRLVHT 285

Query: 915  WTGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHSKAVRDICFSND 1094
            W+GHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHS+AVRDI F ND
Sbjct: 286  WSGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHSQAVRDISFCND 345

Query: 1095 GTKFLSAGYDKNIKYWDTETGQVISTFSTGKVPYVVKLNPDEDKQNVLLAGMSDKKIVQW 1274
            G+KFL+AGYDK IKYWDTETGQVISTFSTGK+PYVVKLNPD+DKQN+LLAGMSDKKIVQW
Sbjct: 346  GSKFLTAGYDKKIKYWDTETGQVISTFSTGKIPYVVKLNPDDDKQNILLAGMSDKKIVQW 405

Query: 1275 DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS 1454
            DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS
Sbjct: 406  DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS 465

Query: 1455 MPSISLHPNSNWLACQSLDNQILIYSTRERFQLNKKKRFAGHIAAGYACQVNFSPDGRFV 1634
            MPSIS+HPN+NWLA QSLDNQILIYSTRERFQLNKKKRFAGHI AGYACQVNFSPDGRFV
Sbjct: 466  MPSISVHPNTNWLAAQSLDNQILIYSTRERFQLNKKKRFAGHIVAGYACQVNFSPDGRFV 525

Query: 1635 MSGDGEGRCWFWDWKSCKVFRTLRCHDGVCIGCEWHPLEQSKVATCGWDGLIKYWD 1802
            MSGDGEG+CWFWDWK+CKVFRTL+CH+GVCIGCEWHPLEQSKVATCGWDGLIKYWD
Sbjct: 526  MSGDGEGKCWFWDWKTCKVFRTLKCHEGVCIGCEWHPLEQSKVATCGWDGLIKYWD 581


>ref|XP_002312609.1| predicted protein [Populus trichocarpa] gi|222852429|gb|EEE89976.1|
            predicted protein [Populus trichocarpa]
          Length = 581

 Score =  928 bits (2399), Expect = 0.0
 Identities = 442/554 (79%), Positives = 476/554 (85%), Gaps = 4/554 (0%)
 Frame = +3

Query: 153  LPAKSAAPKVDDTMLSLTVAGQAHSLTHKPIDPTQHLVSFNPTYDQLWAPIVGPSHPYAK 332
            LP+KSAAPKVDDTML+LTVA Q  S   KPIDP QH V+FNPTYDQLWAP++GP+HPYAK
Sbjct: 33   LPSKSAAPKVDDTMLALTVANQTLS---KPIDPVQHAVAFNPTYDQLWAPVLGPAHPYAK 89

Query: 333  DGLAQGLRNHKLGFVENANIESFAFDEQYNTFYKFGYAADPSQ---NTYVGDVEGFEKNE 503
            DG+AQG+RNHKLGFVENA I+SF FDEQYNTF+K+GYAADPS    N YVGD++  EK  
Sbjct: 90   DGIAQGMRNHKLGFVENAAIDSFVFDEQYNTFHKYGYAADPSASAGNNYVGDLDTLEKTN 149

Query: 504  GISVYNIPQHXXXXXXXXXXXXXXXXXX-GDAEVDKDELGNPATETWLMKNRKSPWAGKR 680
            GISVYNIPQH                   GD  +DK+E+ NPAT+ WLMKNRKSPWAGK+
Sbjct: 150  GISVYNIPQHEQKKRKIEKKREVTEEEGDGDDVMDKEEVENPATDAWLMKNRKSPWAGKK 209

Query: 681  EWVQGELTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDKSTFHGKEEKDYQGRSWIAPPK 860
            E +Q EL+                             DK+TFHGKEE+DYQGRSW+APPK
Sbjct: 210  EGLQTELSEEQQKYAEEHARKKAEKAGGEKGEVVA--DKTTFHGKEERDYQGRSWLAPPK 267

Query: 861  DAKASNDHCYIPKRLVHTWTGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCM 1040
            DAKASNDHCYIPKRLVHTW+GHTKGVSAIRFFPK+GHLILSAGMDTKVKIWDVFNSGKCM
Sbjct: 268  DAKASNDHCYIPKRLVHTWSGHTKGVSAIRFFPKYGHLILSAGMDTKVKIWDVFNSGKCM 327

Query: 1041 RTYMGHSKAVRDICFSNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKVPYVVKLNPDE 1220
            RTYMGHSKAVRDI F NDGTKFL+A YDKNIKYWDTETGQVISTFSTGK+PYVVKLNPD+
Sbjct: 328  RTYMGHSKAVRDISFCNDGTKFLTASYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDD 387

Query: 1221 DKQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVW 1400
            DKQN+LLAGMSDKKIVQWDMN+GQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVW
Sbjct: 388  DKQNILLAGMSDKKIVQWDMNSGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVW 447

Query: 1401 EFGIPVVIKYISEPHMHSMPSISLHPNSNWLACQSLDNQILIYSTRERFQLNKKKRFAGH 1580
            EFGIPVVIKYISEPHMHSMP+ISLHPNSNWLA QSLDNQILIYSTRERFQLNKKKRFAGH
Sbjct: 448  EFGIPVVIKYISEPHMHSMPAISLHPNSNWLAAQSLDNQILIYSTRERFQLNKKKRFAGH 507

Query: 1581 IAAGYACQVNFSPDGRFVMSGDGEGRCWFWDWKSCKVFRTLRCHDGVCIGCEWHPLEQSK 1760
            I AGYACQVNFSPDGRFVMSGDGEG+CWFWDWKSCKVFRTL+CH+GVCIGCEWHPLEQSK
Sbjct: 508  IVAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVCIGCEWHPLEQSK 567

Query: 1761 VATCGWDGLIKYWD 1802
            VATCGWDGLIKYWD
Sbjct: 568  VATCGWDGLIKYWD 581


>ref|XP_004155354.1| PREDICTED: LOW QUALITY PROTEIN: pre-mRNA-processing factor 17-like
            [Cucumis sativus]
          Length = 580

 Score =  928 bits (2398), Expect = 0.0
 Identities = 447/595 (75%), Positives = 487/595 (81%), Gaps = 3/595 (0%)
 Frame = +3

Query: 24   MDLLRSYKESEIDDDEKVDNVXXXXXXXXXXXXXXXXXXXXXXLPAKSAAPKVDDTMLSL 203
            MDLL+SY +   DD                             LP+K++APKVDDTML+L
Sbjct: 1    MDLLQSYTDQNDDDGSPKQ------------LTSSPEASPPRLLPSKTSAPKVDDTMLAL 48

Query: 204  TVAGQAHSLTHKPIDPTQHLVSFNPTYDQLWAPIVGPSHPYAKDGLAQGLRNHKLGFVEN 383
            TVA    +L+ KPIDPTQHLV+FNPTYDQLWAPI GPSHPYAKDG+AQG+RNHKLGFVEN
Sbjct: 49   TVANANQTLS-KPIDPTQHLVAFNPTYDQLWAPIYGPSHPYAKDGIAQGMRNHKLGFVEN 107

Query: 384  ANIESFAFDEQYNTFYKFGYAADPSQ---NTYVGDVEGFEKNEGISVYNIPQHXXXXXXX 554
            A+IE F FDEQYNTF+K+GYAADPS    N Y+GD+E  EKN+ ISVYNIPQH       
Sbjct: 108  ASIEPFVFDEQYNTFHKYGYAADPSASAGNNYIGDMEALEKNDAISVYNIPQHEQKKRKI 167

Query: 555  XXXXXXXXXXXGDAEVDKDELGNPATETWLMKNRKSPWAGKREWVQGELTXXXXXXXXXX 734
                        + EV+  E+ NPA++ WLMKNRKSPW+GK+E +Q ELT          
Sbjct: 168  EKKKKXSENEDMEEEVNPAEVDNPASDVWLMKNRKSPWSGKKEGLQTELTEEQKKYAEEY 227

Query: 735  XXXXXXXXXXXXXXXXXXXDKSTFHGKEEKDYQGRSWIAPPKDAKASNDHCYIPKRLVHT 914
                               DKSTFHGKEE+DYQGRSWIAPPKDAKA+NDHCYIPKRLVHT
Sbjct: 228  AKKKGEEKGGEKGEVTS--DKSTFHGKEERDYQGRSWIAPPKDAKATNDHCYIPKRLVHT 285

Query: 915  WTGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHSKAVRDICFSND 1094
            W+GHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHS+AVRDI F ND
Sbjct: 286  WSGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHSQAVRDISFCND 345

Query: 1095 GTKFLSAGYDKNIKYWDTETGQVISTFSTGKVPYVVKLNPDEDKQNVLLAGMSDKKIVQW 1274
            G+KFL+AGYDK IKYWDTETGQVISTFSTGK+PYVVKLNPD+DKQN+LLAGMSDKKIVQW
Sbjct: 346  GSKFLTAGYDKKIKYWDTETGQVISTFSTGKIPYVVKLNPDDDKQNILLAGMSDKKIVQW 405

Query: 1275 DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS 1454
            DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS
Sbjct: 406  DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS 465

Query: 1455 MPSISLHPNSNWLACQSLDNQILIYSTRERFQLNKKKRFAGHIAAGYACQVNFSPDGRFV 1634
            MPSIS+HPN+NWLA QSLDNQILIYSTRERFQLNKKKRFAGHI AGYACQVNFSPDGRFV
Sbjct: 466  MPSISVHPNTNWLAAQSLDNQILIYSTRERFQLNKKKRFAGHIVAGYACQVNFSPDGRFV 525

Query: 1635 MSGDGEGRCWFWDWKSCKVFRTLRCHDGVCIGCEWHPLEQSKVATCGWDGLIKYW 1799
            MSGDGEG+CWFWDWK+CKVFRTL+CH+GVCIGCEWHPLEQSKVATCGWDGLIKYW
Sbjct: 526  MSGDGEGKCWFWDWKTCKVFRTLKCHEGVCIGCEWHPLEQSKVATCGWDGLIKYW 580


>ref|XP_004135243.1| PREDICTED: pre-mRNA-processing factor 17-like [Cucumis sativus]
          Length = 580

 Score =  928 bits (2398), Expect = 0.0
 Identities = 446/595 (74%), Positives = 486/595 (81%), Gaps = 3/595 (0%)
 Frame = +3

Query: 24   MDLLRSYKESEIDDDEKVDNVXXXXXXXXXXXXXXXXXXXXXXLPAKSAAPKVDDTMLSL 203
            MDLL+SY +   DD                             LP+K++APKVDDTML+L
Sbjct: 1    MDLLQSYTDQNDDDGSPKQ------------LTSSPEASPPRLLPSKTSAPKVDDTMLAL 48

Query: 204  TVAGQAHSLTHKPIDPTQHLVSFNPTYDQLWAPIVGPSHPYAKDGLAQGLRNHKLGFVEN 383
            TVA    +L+ KPIDPTQHLV+FNPTYDQLWAPI GPSHPYAKDG+AQG+RNHKLGFVEN
Sbjct: 49   TVANANQTLS-KPIDPTQHLVAFNPTYDQLWAPIYGPSHPYAKDGIAQGMRNHKLGFVEN 107

Query: 384  ANIESFAFDEQYNTFYKFGYAADPSQ---NTYVGDVEGFEKNEGISVYNIPQHXXXXXXX 554
            A+IE F FDEQYNTF+K+GYAADPS    N Y+GD+E  EKN+ ISVYNIPQH       
Sbjct: 108  ASIEPFVFDEQYNTFHKYGYAADPSASAGNNYIGDMEALEKNDAISVYNIPQHEQKKRKI 167

Query: 555  XXXXXXXXXXXGDAEVDKDELGNPATETWLMKNRKSPWAGKREWVQGELTXXXXXXXXXX 734
                        + EV+  E+ NPA++ WLMKNRKSPW+GK+E +Q ELT          
Sbjct: 168  EKKKEMSENEDMEEEVNPAEVDNPASDVWLMKNRKSPWSGKKEGLQTELTEEQKKYAEEY 227

Query: 735  XXXXXXXXXXXXXXXXXXXDKSTFHGKEEKDYQGRSWIAPPKDAKASNDHCYIPKRLVHT 914
                               DKSTFHGKEE+DYQGRSWIAPPKDAKA+NDHCYIPKRLVHT
Sbjct: 228  AKKKGEEKGGEKGEVTS--DKSTFHGKEERDYQGRSWIAPPKDAKATNDHCYIPKRLVHT 285

Query: 915  WTGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHSKAVRDICFSND 1094
            W+GHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHS+AVRDI F ND
Sbjct: 286  WSGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMRTYMGHSQAVRDISFCND 345

Query: 1095 GTKFLSAGYDKNIKYWDTETGQVISTFSTGKVPYVVKLNPDEDKQNVLLAGMSDKKIVQW 1274
            G+KFL+AGYDK IKYWDTETGQVISTFSTGK+PYVVKLNPD+DKQN+LLAGMSDKKIVQW
Sbjct: 346  GSKFLTAGYDKKIKYWDTETGQVISTFSTGKIPYVVKLNPDDDKQNILLAGMSDKKIVQW 405

Query: 1275 DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS 1454
            DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS
Sbjct: 406  DMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEFGIPVVIKYISEPHMHS 465

Query: 1455 MPSISLHPNSNWLACQSLDNQILIYSTRERFQLNKKKRFAGHIAAGYACQVNFSPDGRFV 1634
            MPSIS+HPN+NWLA QSLDNQILIYSTRERFQLNKKKRFAGHI AGYACQVNFSPDGRFV
Sbjct: 466  MPSISVHPNTNWLAAQSLDNQILIYSTRERFQLNKKKRFAGHIVAGYACQVNFSPDGRFV 525

Query: 1635 MSGDGEGRCWFWDWKSCKVFRTLRCHDGVCIGCEWHPLEQSKVATCGWDGLIKYW 1799
            MSGDGEG+CWFWDWK+CKVFRTL+CH+GVCIGCEWHPLEQSKVATCGWDGLIKYW
Sbjct: 526  MSGDGEGKCWFWDWKTCKVFRTLKCHEGVCIGCEWHPLEQSKVATCGWDGLIKYW 580


>ref|XP_002262666.1| PREDICTED: pre-mRNA-processing factor 17-like [Vitis vinifera]
          Length = 583

 Score =  926 bits (2394), Expect = 0.0
 Identities = 442/552 (80%), Positives = 473/552 (85%), Gaps = 3/552 (0%)
 Frame = +3

Query: 153  LPAKSAAPKVDDTMLSLTVAGQAHSLTHKPIDPTQHLVSFNPTYDQLWAPIVGPSHPYAK 332
            LP+KS+APKVDDT L+L +A    +   KPIDP QH+V FNPTYDQLWAPI GP+HPYAK
Sbjct: 39   LPSKSSAPKVDDTTLALAIA----NTNDKPIDPIQHVVPFNPTYDQLWAPIYGPAHPYAK 94

Query: 333  DGLAQGLRNHKLGFVENANIESFAFDEQYNTFYKFGYAADPSQ---NTYVGDVEGFEKNE 503
            DG+AQG+RNHKLGFVENA IE F FDEQYNTF+K+GYAADPS    N YVGD+E  EKN+
Sbjct: 95   DGIAQGMRNHKLGFVENAAIEPFIFDEQYNTFHKYGYAADPSASAGNNYVGDLEALEKND 154

Query: 504  GISVYNIPQHXXXXXXXXXXXXXXXXXXGDAEVDKDELGNPATETWLMKNRKSPWAGKRE 683
            GISVYNIPQH                   D  VD +E+ NPAT+TWL+KN+KSPWAGK+E
Sbjct: 155  GISVYNIPQHEQKKRKIEKKKESESEENED--VDVEEVQNPATDTWLLKNKKSPWAGKKE 212

Query: 684  WVQGELTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDKSTFHGKEEKDYQGRSWIAPPKD 863
             +Q ELT                             DKSTFHGKEE+DYQGRSWIAPPKD
Sbjct: 213  GLQTELTEEQKKYAEEYAKKKGEEKGAAEKGEFVT-DKSTFHGKEERDYQGRSWIAPPKD 271

Query: 864  AKASNDHCYIPKRLVHTWTGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMR 1043
            AKA+NDHCYIPKRLVHTW+GHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMR
Sbjct: 272  AKATNDHCYIPKRLVHTWSGHTKGVSAIRFFPKHGHLILSAGMDTKVKIWDVFNSGKCMR 331

Query: 1044 TYMGHSKAVRDICFSNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKVPYVVKLNPDED 1223
            TYMGHSKAVRDI F NDGTKFL+AGYDKNIKYWDTETGQVISTFSTGK+PYVVKLNPD+D
Sbjct: 332  TYMGHSKAVRDISFCNDGTKFLTAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDDD 391

Query: 1224 KQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWE 1403
            KQN+LLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWE
Sbjct: 392  KQNILLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWE 451

Query: 1404 FGIPVVIKYISEPHMHSMPSISLHPNSNWLACQSLDNQILIYSTRERFQLNKKKRFAGHI 1583
            FGIPVVIKYISEPHMHSMPSISLHPN+NWLA QSLDNQILIYSTRERFQLNKKKRFAGHI
Sbjct: 452  FGIPVVIKYISEPHMHSMPSISLHPNTNWLAAQSLDNQILIYSTRERFQLNKKKRFAGHI 511

Query: 1584 AAGYACQVNFSPDGRFVMSGDGEGRCWFWDWKSCKVFRTLRCHDGVCIGCEWHPLEQSKV 1763
            AAGYACQVNFSPDGRFVMSGDGEG+CWFWDWKSCKVFRTL+CH+GVCIGCEWHPLEQSKV
Sbjct: 512  AAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVCIGCEWHPLEQSKV 571

Query: 1764 ATCGWDGLIKYW 1799
            ATCGWDGLIKYW
Sbjct: 572  ATCGWDGLIKYW 583