BLASTX nr result
ID: Scutellaria23_contig00000287
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Scutellaria23_contig00000287
(1572 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK84485.1| putative thermostable pectinesterase [Citrus sine... 635 0.0
ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|2... 602 e-174
gb|AFB77928.1| pectin methylesterase [Gossypium hirsutum] 596 e-171
gb|AFQ23194.1| pectin methylesterase [Theobroma cacao] 601 e-170
ref|XP_002532944.1| Pectinesterase-3 precursor, putative [Ricinu... 600 e-170
>gb|AAK84485.1| putative thermostable pectinesterase [Citrus sinensis]
gi|24250751|gb|AAK84486.1| putative thermostable
pectinesterase [Citrus sinensis]
Length = 631
Score = 635 bits (1639), Expect(2) = 0.0
Identities = 321/453 (70%), Positives = 365/453 (80%), Gaps = 7/453 (1%)
Frame = +3
Query: 231 AVAPTSLKSSKDVLITALNYTIYTVERNYFAVKKIGASRAGSLTRREKTALHDCLEMVSD 410
A A + + S KDV+ +LN TI V+ NYFA+KK+ +R +LT+REKT+LHDCLEMV +
Sbjct: 133 AAAVSKVNSPKDVIRLSLNLTITAVQHNYFAIKKLITTRKSTLTKREKTSLHDCLEMVDE 192
Query: 411 TLEELRESEVELKDY--GFKNYSISDHKENLITLLSAAQTNQESCLDGFSHGEADKKVRQ 584
TL+EL ++E EL+ Y N SI++ + L L+SAA TNQE+CLDGFSH ADKK+R+
Sbjct: 193 TLDELYKTEHELQGYPAAANNKSIAEQADELKILVSAAMTNQETCLDGFSHERADKKIRE 252
Query: 585 ALVAGQMHVFRLVSNVMAMVKNISDSERASS---ELIRERRLGEE--WPEWLSAGDRRLL 749
L+ GQMHVF + SN +AM+KN++D + + RRL +E WPEWLSAGDRRLL
Sbjct: 253 ELMEGQMHVFHMCSNALAMIKNMTDGDIGKDIVDHYSKARRLDDETKWPEWLSAGDRRLL 312
Query: 750 QXXXXXXXXXXXXXXXXNYRTVAEAVAAAPEGSSRRYVIRIKAGVYRENVEIGRRKTNLM 929
Q NY TVA AVAAAPEGSSRRY+IRIKAG YRENVE+ ++K NLM
Sbjct: 313 QATTVVPDVTVAADGSGNYLTVAAAVAAAPEGSSRRYIIRIKAGEYRENVEVPKKKINLM 372
Query: 930 FVGDGRTTTIITASRNVVDGSTTFNSATVAVVGGGFLARDITFQNTAGPSKHQAVALRVN 1109
F+GDGRTTTIIT SRNVVDGSTTFNSATVAVVG GFLARDITFQNTAGPSKHQAVALRV
Sbjct: 373 FIGDGRTTTIITGSRNVVDGSTTFNSATVAVVGDGFLARDITFQNTAGPSKHQAVALRVG 432
Query: 1110 ADLCAFYRCDMLAYQDTLYVHSLRQFYTGCIIVGTVDFIFGNANAVLQNCDIHARRPNPG 1289
+DL AFYRCDMLAYQDTLYVHSLRQFYT CII GTVDFIFGNA AV QNCDIHARRPNP
Sbjct: 433 SDLSAFYRCDMLAYQDTLYVHSLRQFYTSCIIAGTVDFIFGNAAAVFQNCDIHARRPNPN 492
Query: 1290 QRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQTEI 1469
QRNMVTAQGRDDPNQNTGIVIQKCRIGATSDL V+ SF TYLGRPWK YSRTVVMQ++I
Sbjct: 493 QRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLLAVKGSFETYLGRPWKRYSRTVVMQSDI 552
Query: 1470 SDVIHPAGWFEWNGNFALDTLFYAEYQNTGAGA 1568
SDVI+PAGW+EW+GNFALDTLFYAEYQNTGAGA
Sbjct: 553 SDVINPAGWYEWSGNFALDTLFYAEYQNTGAGA 585
Score = 34.3 bits (77), Expect(2) = 0.0
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2
Query: 62 SHGGAAAQAVVKSTCAATLYPEL 130
+H A AQ V++S+C+ATLYPEL
Sbjct: 101 AHAHAYAQPVIRSSCSATLYPEL 123
>ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|222841689|gb|EEE79236.1|
predicted protein [Populus trichocarpa]
Length = 579
Score = 602 bits (1553), Expect(2) = e-174
Identities = 302/452 (66%), Positives = 368/452 (81%), Gaps = 7/452 (1%)
Frame = +3
Query: 237 APTSLKSSKDVLITALNYTIYTVERNYFAVKKIGASRAGSLTRREKTALHDCLEMVSDTL 416
A ++L S KDV+ ++N T V+ N+F V+K+ A++ LT+REKTALHDCLE++ +TL
Sbjct: 86 ATSNLASQKDVIELSINLTTKAVQHNFFTVEKLIATK--KLTKREKTALHDCLEIIDETL 143
Query: 417 EELRESEVELKDYGFKNYSISDHKENLITLLSAAQTNQESCLDGFSHGEADKKVRQALVA 596
+EL E+ V+L DY N S+ H ++L TLLS+A TNQE+CLDGFSH EADKKVR+AL+
Sbjct: 144 DELHEALVDLNDYP-NNKSLKKHADDLKTLLSSAITNQETCLDGFSHDEADKKVRKALLK 202
Query: 597 GQMHVFRLVSNVMAMVKNISDSERASS------ELIRERRLGE-EWPEWLSAGDRRLLQX 755
GQ+HV ++ SNV+AM+KN++D++ A+ +L++E+ E EWPEW+S DRRLLQ
Sbjct: 203 GQIHVEKMCSNVLAMIKNMTDTDVANELKTTNRKLMQEKEGNESEWPEWMSVADRRLLQS 262
Query: 756 XXXXXXXXXXXXXXXNYRTVAEAVAAAPEGSSRRYVIRIKAGVYRENVEIGRRKTNLMFV 935
NY+TV+ AVAAAP+ SS+RY+IRIKAGVYRENV++ + KTN+MF+
Sbjct: 263 SSVTPDVVVAADGSGNYKTVSAAVAAAPKKSSKRYIIRIKAGVYRENVDVPKDKTNIMFM 322
Query: 936 GDGRTTTIITASRNVVDGSTTFNSATVAVVGGGFLARDITFQNTAGPSKHQAVALRVNAD 1115
GDGR TTIITASRNVVDGSTTFNSATVA VG GFLAR ITFQNTAGPSKHQAVALRV +D
Sbjct: 323 GDGRKTTIITASRNVVDGSTTFNSATVAAVGQGFLARGITFQNTAGPSKHQAVALRVGSD 382
Query: 1116 LCAFYRCDMLAYQDTLYVHSLRQFYTGCIIVGTVDFIFGNANAVLQNCDIHARRPNPGQR 1295
L AFY CDMLAYQDTLYVHS RQF+ C++ GTVDFIFGNA VLQ+CDIHARRPN GQ+
Sbjct: 383 LSAFYDCDMLAYQDTLYVHSNRQFFINCLVAGTVDFIFGNAAVVLQDCDIHARRPNSGQK 442
Query: 1296 NMVTAQGRDDPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQTEISD 1475
NMVTAQGR DPNQNTGIVIQK RIGATSDL+PV+SSFPTYLGRPWKEYSRTV+MQ+ I+D
Sbjct: 443 NMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVKSSFPTYLGRPWKEYSRTVIMQSSITD 502
Query: 1476 VIHPAGWFEWNGNFALDTLFYAEYQNTGAGAG 1571
VI PAGWFEW+G+FAL+TL+YAEYQN+GAGAG
Sbjct: 503 VIQPAGWFEWSGSFALNTLYYAEYQNSGAGAG 534
Score = 36.2 bits (82), Expect(2) = e-174
Identities = 15/28 (53%), Positives = 20/28 (71%)
Frame = +2
Query: 47 VQSHKSHGGAAAQAVVKSTCAATLYPEL 130
V SHKS A++KS+C++TLYPEL
Sbjct: 48 VNSHKSSKNEGTHAILKSSCSSTLYPEL 75
>gb|AFB77928.1| pectin methylesterase [Gossypium hirsutum]
Length = 567
Score = 596 bits (1536), Expect(2) = e-171
Identities = 300/450 (66%), Positives = 358/450 (79%), Gaps = 6/450 (1%)
Frame = +3
Query: 237 APTSLKSSKDVLITALNYTIYTVERNYFAVKKIGASRAGSLTRREKTALHDCLEMVSDTL 416
A T +K+ KDV+ +LN T+ V+ NY ++KK+ +++ SLT REK AL+DCLE+V +TL
Sbjct: 72 AETKVKNPKDVIELSLNLTVTAVQSNYLSIKKLISTQRKSLTEREKAALNDCLELVDETL 131
Query: 417 EELRESEVELKDYGFKNYSISDHKENLITLLSAAQTNQESCLDGFSHGEADKKVRQALVA 596
+EL +E +L DY N SIS H ++L +LLSAA TNQE+CLDGFSH +ADKKVRQAL+
Sbjct: 132 DELFVAEHDLSDYPSFNKSISQHADDLKSLLSAAMTNQETCLDGFSHDKADKKVRQALLD 191
Query: 597 GQMHVFRLVSNVMAMVKNISDSERASSELIRE--RRLGE----EWPEWLSAGDRRLLQXX 758
GQMHVF + SN +AM+KN++D++ AS R+L E EWP+WLS GDRRLLQ
Sbjct: 192 GQMHVFHMCSNALAMIKNLTDTDMASQGYHPSSGRQLEEQDQTEWPKWLSEGDRRLLQAT 251
Query: 759 XXXXXXXXXXXXXXNYRTVAEAVAAAPEGSSRRYVIRIKAGVYRENVEIGRRKTNLMFVG 938
++ TV+EAVAAAPE S+ RY+I+IKAGVYRENV++ +KTNLMFVG
Sbjct: 252 TVIPNVTVAADGSGDFLTVSEAVAAAPERSTTRYIIKIKAGVYRENVDVPSKKTNLMFVG 311
Query: 939 DGRTTTIITASRNVVDGSTTFNSATVAVVGGGFLARDITFQNTAGPSKHQAVALRVNADL 1118
DGR TIITASRNVVDGSTTF+SATVA VG GFLARDITFQNTAGPSKHQAVALRV +DL
Sbjct: 312 DGRVNTIITASRNVVDGSTTFHSATVAAVGDGFLARDITFQNTAGPSKHQAVALRVGSDL 371
Query: 1119 CAFYRCDMLAYQDTLYVHSLRQFYTGCIIVGTVDFIFGNANAVLQNCDIHARRPNPGQRN 1298
AFYRC +LAYQDTLYVHSLRQFY+ C++ G+VDFIFGNA AVLQ+CDIHARRPNP QRN
Sbjct: 372 SAFYRCGILAYQDTLYVHSLRQFYSQCLVAGSVDFIFGNAAAVLQDCDIHARRPNPNQRN 431
Query: 1299 MVTAQGRDDPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQTEISDV 1478
MVTAQGR DPN+NTGIVIQKCRIGATSDL V+S F TYLGRPWK +SRTV+MQ+ ISD+
Sbjct: 432 MVTAQGRSDPNENTGIVIQKCRIGATSDLEAVKSDFETYLGRPWKTHSRTVIMQSVISDI 491
Query: 1479 IHPAGWFEWNGNFALDTLFYAEYQNTGAGA 1568
IHPAGWF W+ +FALDTL Y EYQNTG GA
Sbjct: 492 IHPAGWFPWDKDFALDTLTYREYQNTGPGA 521
Score = 33.9 bits (76), Expect(2) = e-171
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 47 VQSHKSHGGAAAQAVVKSTCAATLYPEL 130
+ KS AA +++KS+C++TLYPEL
Sbjct: 34 ISKKKSSNTVAAHSIIKSSCSSTLYPEL 61
>gb|AFQ23194.1| pectin methylesterase [Theobroma cacao]
Length = 582
Score = 601 bits (1549), Expect(2) = e-170
Identities = 308/457 (67%), Positives = 359/457 (78%), Gaps = 9/457 (1%)
Frame = +3
Query: 228 AAVAPTS--LKSSKDVLITALNYTIYTVERNYFAVKKIGASRAGSLTRREKTALHDCLEM 401
AA A TS +KS KDV+ +LN T VE NYF +KK+ A + LT REKTALHDCLE
Sbjct: 84 AAPAATSKKVKSKKDVIELSLNITTTAVEHNYFKIKKLLAKKG--LTEREKTALHDCLET 141
Query: 402 VSDTLEELRESEVELKDYGFKNYSISDHKENLITLLSAAQTNQESCLDGFSHGEADKKVR 581
+ +TL+EL E+ +L +Y K S++ H ++L TL+SAA TNQE+CLDGFSH ADKK+R
Sbjct: 142 IDETLDELHEAVEDLHEYPNKK-SLTQHADDLKTLMSAAMTNQETCLDGFSHDAADKKIR 200
Query: 582 QALVAGQMHVFRLVSNVMAMVKNISDSERASSEL-------IRERRLGEEWPEWLSAGDR 740
+ L+ G+ +V R+ SN +AM+KN++D++ A+ L ++E+ G WPEWLSAGDR
Sbjct: 201 KVLIDGEKYVERMCSNALAMIKNMTDTDIANEMLKTSSNRKLKEQENGIAWPEWLSAGDR 260
Query: 741 RLLQXXXXXXXXXXXXXXXXNYRTVAEAVAAAPEGSSRRYVIRIKAGVYRENVEIGRRKT 920
RLLQ NY+TV+EAVA AP+ SS+RYVI+IKAGVYRENVE+ ++KT
Sbjct: 261 RLLQSSSVTPDVVVAADGSGNYKTVSEAVAKAPQRSSKRYVIKIKAGVYRENVEVPKKKT 320
Query: 921 NLMFVGDGRTTTIITASRNVVDGSTTFNSATVAVVGGGFLARDITFQNTAGPSKHQAVAL 1100
N+MF+GDGRT TIIT SRNVVDGSTTF+SATVAVVG FLAR ITFQNTAGPSKHQAVAL
Sbjct: 321 NIMFLGDGRTETIITGSRNVVDGSTTFHSATVAVVGERFLARSITFQNTAGPSKHQAVAL 380
Query: 1101 RVNADLCAFYRCDMLAYQDTLYVHSLRQFYTGCIIVGTVDFIFGNANAVLQNCDIHARRP 1280
RV ADL AFY CDMLAYQDTLY HS RQFY CII GTVDFIFGNA AV QNCDIHARRP
Sbjct: 381 RVGADLSAFYECDMLAYQDTLYAHSNRQFYVNCIIAGTVDFIFGNAAAVFQNCDIHARRP 440
Query: 1281 NPGQRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQ 1460
N GQ+NMVTAQGR DPNQNTGIVIQKCRIGATSDL+ V S+FPTYLGRPWKEYSRTVVMQ
Sbjct: 441 NSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVTSNFPTYLGRPWKEYSRTVVMQ 500
Query: 1461 TEISDVIHPAGWFEWNGNFALDTLFYAEYQNTGAGAG 1571
+ ISDVIHPAGW EW+ +FAL TLFY EYQNTGAGAG
Sbjct: 501 SVISDVIHPAGWHEWSESFALRTLFYGEYQNTGAGAG 537
Score = 24.6 bits (52), Expect(2) = e-170
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2
Query: 86 AVVKSTCAATLYPEL 130
A+VKS C+ T YP+L
Sbjct: 63 AIVKSACSITRYPDL 77
>ref|XP_002532944.1| Pectinesterase-3 precursor, putative [Ricinus communis]
gi|223527273|gb|EEF29428.1| Pectinesterase-3 precursor,
putative [Ricinus communis]
Length = 577
Score = 600 bits (1546), Expect(2) = e-170
Identities = 306/454 (67%), Positives = 361/454 (79%), Gaps = 9/454 (1%)
Frame = +3
Query: 237 APTSLKSSKDVLITALNYTIYTVERNYFAVKKIGASRAGSLTRREKTALHDCLEMVSDTL 416
A + L S KDV+ +LN T VE N+F V+K+ A R G LT+REKTALHDCLE + +TL
Sbjct: 87 ATSKLVSLKDVIEVSLNLTTTAVEHNFFTVEKL-AKRKG-LTKREKTALHDCLETIDETL 144
Query: 417 EELRESEVELKDYGFKNYSISDHKENLITLLSAAQTNQESCLDGFSHGEADKKVRQALVA 596
+EL ++ +LK+Y K S+S H ++L TLLS+A TNQE+CLDGFSH ADK +R+AL+A
Sbjct: 145 DELHKAMDDLKEYPNKK-SLSQHADDLKTLLSSAITNQETCLDGFSHDGADKHIREALLA 203
Query: 597 GQMHVFRLVSNVMAMVKNISDSERASSELI---RERRLGEE------WPEWLSAGDRRLL 749
GQ+HV ++ SN +AM+KN++D++ A+ + + R+L EE WPEWLSAGDRRLL
Sbjct: 204 GQVHVEKMCSNALAMIKNMTDTDIANELKLSGSKNRKLKEEKDQESVWPEWLSAGDRRLL 263
Query: 750 QXXXXXXXXXXXXXXXXNYRTVAEAVAAAPEGSSRRYVIRIKAGVYRENVEIGRRKTNLM 929
Q +Y+TV+ AVAAAP SS+RY+IRIKAGVY+ENVE+ + KTNLM
Sbjct: 264 QSSSVTPNVVVAADGSGDYKTVSAAVAAAPSKSSKRYIIRIKAGVYKENVEVPKGKTNLM 323
Query: 930 FVGDGRTTTIITASRNVVDGSTTFNSATVAVVGGGFLARDITFQNTAGPSKHQAVALRVN 1109
F+GDGR TTIIT SRNVVDGSTTFNSATVA VG GFLAR +TF+N AGPSKHQAVALRV
Sbjct: 324 FLGDGRKTTIITGSRNVVDGSTTFNSATVAAVGQGFLARGVTFENKAGPSKHQAVALRVG 383
Query: 1110 ADLCAFYRCDMLAYQDTLYVHSLRQFYTGCIIVGTVDFIFGNANAVLQNCDIHARRPNPG 1289
ADL AFY CDM+AYQDTLYVHS RQF+ C I GTVDFIFGN+ AV Q+CDIHAR+PN G
Sbjct: 384 ADLAAFYECDMIAYQDTLYVHSNRQFFINCYIAGTVDFIFGNSAAVFQDCDIHARKPNSG 443
Query: 1290 QRNMVTAQGRDDPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQTEI 1469
Q+NMVTAQGR DPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQT I
Sbjct: 444 QKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQSSFPTYLGRPWKEYSRTVVMQTAI 503
Query: 1470 SDVIHPAGWFEWNGNFALDTLFYAEYQNTGAGAG 1571
SDVIHPAGW EW+G+FAL TLFY EYQN+GAGAG
Sbjct: 504 SDVIHPAGWHEWSGSFALSTLFYGEYQNSGAGAG 537
Score = 25.8 bits (55), Expect(2) = e-170
Identities = 12/32 (37%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
Frame = +2
Query: 38 ITIVQSHKSHGGAA-AQAVVKSTCAATLYPEL 130
+T V S+K A+ + +++KS+C+ T YP+L
Sbjct: 45 VTGVNSNKKDTTASPSHSILKSSCSTTRYPDL 76