BLASTX nr result
ID: Scutellaria22_contig00002960
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Scutellaria22_contig00002960
(1736 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFK79031.1| cytochrome P450 CYP736A54 [Bupleurum chinense] 288 3e-88
ref|XP_002310016.1| cytochrome P450 [Populus trichocarpa] gi|222... 267 2e-81
ref|XP_002310014.1| cytochrome P450 [Populus trichocarpa] gi|222... 267 2e-81
ref|XP_002310008.1| cytochrome P450 [Populus trichocarpa] gi|222... 272 8e-81
ref|XP_003524281.1| PREDICTED: cytochrome P450 71A1-like [Glycin... 268 5e-80
>gb|AFK79031.1| cytochrome P450 CYP736A54 [Bupleurum chinense]
Length = 497
Score = 288 bits (738), Expect(2) = 3e-88
Identities = 141/270 (52%), Positives = 196/270 (72%), Gaps = 6/270 (2%)
Frame = -2
Query: 1687 MVWIW-SILSVLVFVYVVQKLLFGPKSNKKVPPGPRGLPILGHFHLLSSNPHHDLRNMAR 1511
M W W ++L+++V Y+VQ L K+ +K+PPGP+GLPI+GH H+L NPH DL+ +A
Sbjct: 1 MAWTWITLLTLVVLAYLVQSWL-KKKTQRKLPPGPKGLPIIGHLHMLGKNPHQDLQKLAE 59
Query: 1510 KHGPIMLIRFGYVPTVVITSPAAAELILKTHDHVFASRPRNDAAKYIGYEQRNLVSGPYG 1331
KHGPIM +RFG+VP ++++SP AA+ LKTHD FA RP +AAKYI YEQRNL YG
Sbjct: 60 KHGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTYG 119
Query: 1330 PYWRNMRKLVAWELLSSHRITHFQPMRNAEIRLMVDSLRRAEE---AVDLSKTISGLSGD 1160
PYWRNMRKL ELLS+ +I FQ MR EI +VD + +A + AVD+S+ IS ++ D
Sbjct: 120 PYWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISQRISSMNSD 179
Query: 1159 MNCLMVFGRKYSDLE--QIGFKSVIAETMELAARFNLADYFPYIGVLDIQGINRRLKHLS 986
++C MVFG+K+ D E + GFK VI M++A FNL DYFPY+G LD+QG+N+R+K ++
Sbjct: 180 ISCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKRMKAIA 239
Query: 985 KIFDGIVEKIINDHAQNKHKDKENEDFVDT 896
K++D +EKI++DH Q+K + + +DFVDT
Sbjct: 240 KVWDQFLEKILDDHDQSK-EPGQTKDFVDT 268
Score = 65.5 bits (158), Expect(2) = 3e-88
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = -3
Query: 795 AGTDTSATSIEWTMSELLRHPHVMKKLKKELESVVGLGNMVDET 664
A DTS+T+IEWT+SELLRHP VM K++KELE VVG+ MV+E+
Sbjct: 297 ASADTSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEES 340
Score = 145 bits (366), Expect = 3e-32
Identities = 65/110 (59%), Positives = 81/110 (73%)
Frame = -1
Query: 518 SQIIVNAWAIMRDPNVWPDPDTFNPERFVGMDIDVRGLDFKLIPFGSGRRGCPGMXXXXX 339
S+++VN WAI RD NVW D + F PERF+G +ID+RG DF+L+PFGSGRRGCPGM
Sbjct: 385 SRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNIDLRGRDFELLPFGSGRRGCPGMQLGLT 444
Query: 338 XXXXXXXXXVHCFDWDLPDGMQPADLDMSEHFGIVTNRAKPLMAIPKYRL 189
+HCFDWDLP+GMQP++LDM+E FG++ RAK LMAIP RL
Sbjct: 445 VVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGLLVGRAKHLMAIPTCRL 494
>ref|XP_002310016.1| cytochrome P450 [Populus trichocarpa] gi|222852919|gb|EEE90466.1|
cytochrome P450 [Populus trichocarpa]
Length = 497
Score = 267 bits (683), Expect(2) = 2e-81
Identities = 136/269 (50%), Positives = 183/269 (68%), Gaps = 6/269 (2%)
Frame = -2
Query: 1687 MVWIWSILSVLVFVYVVQKLLFGPK-SNKKVPPGPRGLPILGHFHLLSSNPHHDLRNMAR 1511
M WI + L+++ + ++ L K + K+PPGP G PI G HLL PHHDL +A+
Sbjct: 1 MAWILTTLALIALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHHDLHQLAK 60
Query: 1510 KHGPIMLIRFGYVPTVVITSPAAAELILKTHDHVFASRPRNDAAKYIGYEQRNLVSGPYG 1331
K+GPIM +R G VPTVV++SP AAELILKT+D VFASRPRN+AAK+I YEQ+NL PYG
Sbjct: 61 KYGPIMYMRLGLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPYG 120
Query: 1330 PYWRNMRKLVAWELLSSHRITHFQPMRNAEIRLMVDSLR---RAEEAVDLSKTISGLSGD 1160
YWRN+RK+ ELLS+H+I F R E+ L++D ++ R AVDL +S LS D
Sbjct: 121 SYWRNVRKMCTLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSAD 180
Query: 1159 MNCLMVFGRKY--SDLEQIGFKSVIAETMELAARFNLADYFPYIGVLDIQGINRRLKHLS 986
++C MVFG+KY + ++ GFK V E M LAA FNL DY P I LD+QG+ +R+K +
Sbjct: 181 ISCRMVFGKKYLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAVG 240
Query: 985 KIFDGIVEKIINDHAQNKHKDKENEDFVD 899
K+FD EKII++H Q K +++ +DFVD
Sbjct: 241 KVFDDFFEKIIDEHIQFKDENR-TKDFVD 268
Score = 63.9 bits (154), Expect(2) = 2e-81
Identities = 27/45 (60%), Positives = 37/45 (82%)
Frame = -3
Query: 798 IAGTDTSATSIEWTMSELLRHPHVMKKLKKELESVVGLGNMVDET 664
+ DTSAT+IEWT+SEL+RHP VMKK++KELE +G+ MV+E+
Sbjct: 297 VGSMDTSATAIEWTLSELIRHPRVMKKVQKELEEKIGMDRMVEES 341
Score = 134 bits (337), Expect = 8e-29
Identities = 62/113 (54%), Positives = 78/113 (69%)
Frame = -1
Query: 521 KSQIIVNAWAIMRDPNVWPDPDTFNPERFVGMDIDVRGLDFKLIPFGSGRRGCPGMXXXX 342
K+++IVN WAI RD + W D + F PERF G +IDVRG DF+L+PFG+GRRGCPGM
Sbjct: 385 KTRVIVNVWAIGRDQSAWTDANKFIPERFAGSNIDVRGRDFQLLPFGAGRRGCPGMHLGL 444
Query: 341 XXXXXXXXXXVHCFDWDLPDGMQPADLDMSEHFGIVTNRAKPLMAIPKYRLNI 183
VHCFDW+LP+ M P +LDM+E FG+VT RA L A P YRL++
Sbjct: 445 TMVRQIVAQLVHCFDWELPNNMLPEELDMTEAFGLVTPRANHLCATPTYRLHL 497
>ref|XP_002310014.1| cytochrome P450 [Populus trichocarpa] gi|222852917|gb|EEE90464.1|
cytochrome P450 [Populus trichocarpa]
Length = 497
Score = 267 bits (683), Expect(2) = 2e-81
Identities = 136/269 (50%), Positives = 183/269 (68%), Gaps = 6/269 (2%)
Frame = -2
Query: 1687 MVWIWSILSVLVFVYVVQKLLFGPK-SNKKVPPGPRGLPILGHFHLLSSNPHHDLRNMAR 1511
M WI + L+++ + ++ L K + K+PPGP G PI G HLL PHHDL +A+
Sbjct: 1 MAWILTTLALIALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHHDLHQLAK 60
Query: 1510 KHGPIMLIRFGYVPTVVITSPAAAELILKTHDHVFASRPRNDAAKYIGYEQRNLVSGPYG 1331
K+GPIM +R G VPTVV++SP AAELILKT+D VFASRPRN+AAK+I YEQ+NL PYG
Sbjct: 61 KYGPIMYMRLGLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPYG 120
Query: 1330 PYWRNMRKLVAWELLSSHRITHFQPMRNAEIRLMVDSLR---RAEEAVDLSKTISGLSGD 1160
YWRN+RK+ ELLS+H+I F R E+ L++D ++ R AVDL +S LS D
Sbjct: 121 SYWRNVRKMCTLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSAD 180
Query: 1159 MNCLMVFGRKY--SDLEQIGFKSVIAETMELAARFNLADYFPYIGVLDIQGINRRLKHLS 986
++C MVFG+KY + ++ GFK V E M LAA FNL DY P I LD+QG+ +R+K +
Sbjct: 181 ISCRMVFGKKYLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAVG 240
Query: 985 KIFDGIVEKIINDHAQNKHKDKENEDFVD 899
K+FD EKII++H Q K +++ +DFVD
Sbjct: 241 KVFDDFFEKIIDEHIQFKDENR-TKDFVD 268
Score = 63.9 bits (154), Expect(2) = 2e-81
Identities = 27/45 (60%), Positives = 37/45 (82%)
Frame = -3
Query: 798 IAGTDTSATSIEWTMSELLRHPHVMKKLKKELESVVGLGNMVDET 664
+ DTSAT+IEWT+SEL+RHP VMKK++KELE +G+ MV+E+
Sbjct: 297 VGSMDTSATAIEWTLSELIRHPRVMKKVQKELEEKIGMDRMVEES 341
Score = 134 bits (337), Expect = 8e-29
Identities = 62/113 (54%), Positives = 78/113 (69%)
Frame = -1
Query: 521 KSQIIVNAWAIMRDPNVWPDPDTFNPERFVGMDIDVRGLDFKLIPFGSGRRGCPGMXXXX 342
K+++IVN WAI RD + W D + F PERF G +IDVRG DF+L+PFG+GRRGCPGM
Sbjct: 385 KTRVIVNVWAIGRDQSAWTDANKFIPERFAGSNIDVRGRDFQLLPFGAGRRGCPGMHLGL 444
Query: 341 XXXXXXXXXXVHCFDWDLPDGMQPADLDMSEHFGIVTNRAKPLMAIPKYRLNI 183
VHCFDW+LP+ M P +LDM+E FG+VT RA L A P YRL++
Sbjct: 445 TMVRQIVAQLVHCFDWELPNNMLPEELDMTEAFGLVTPRANHLCATPTYRLHL 497
>ref|XP_002310008.1| cytochrome P450 [Populus trichocarpa] gi|222852911|gb|EEE90458.1|
cytochrome P450 [Populus trichocarpa]
Length = 493
Score = 272 bits (695), Expect(2) = 8e-81
Identities = 137/268 (51%), Positives = 186/268 (69%), Gaps = 5/268 (1%)
Frame = -2
Query: 1687 MVWIWSILSVLVFVYVVQKLLFGPKSNKKVPPGPRGLPILGHFHLLSSNPHHDLRNMARK 1508
M WIW+ L+ + ++++Q L NK++PPGPRG PI G HLL PH L +A+K
Sbjct: 1 MAWIWTSLAFVALIFLLQWL---STKNKRLPPGPRGFPIFGSLHLLGKFPHRALHQLAQK 57
Query: 1507 HGPIMLIRFGYVPTVVITSPAAAELILKTHDHVFASRPRNDAAKYIGYEQRNLVSGPYGP 1328
+GPIM +R G VPT+V++SP AAEL LKTHD VFA RP +++A+YI Y Q+ + YG
Sbjct: 58 YGPIMHLRLGLVPTIVVSSPEAAELFLKTHDLVFAGRPPHESARYISYGQKGMAFAQYGS 117
Query: 1327 YWRNMRKLVAWELLSSHRITHFQPMRNAEIRLMVDSLRRAEE---AVDLSKTISGLSGDM 1157
YWRN+RK+ ELLSS +IT F+PMR E+ L++ ++ A + AVDLS +S LS DM
Sbjct: 118 YWRNIRKMCTVELLSSLKITSFKPMRMEELDLLIKYIQEAAQERVAVDLSAKVSSLSADM 177
Query: 1156 NCLMVFGRKY--SDLEQIGFKSVIAETMELAARFNLADYFPYIGVLDIQGINRRLKHLSK 983
+C MVFG+KY DL++ GFKSV+ E M L+A NL DY P IG LD+QG+ +R+K +SK
Sbjct: 178 SCRMVFGKKYLDEDLDERGFKSVMQEVMHLSAAPNLGDYIPQIGALDLQGLTKRMKAISK 237
Query: 982 IFDGIVEKIINDHAQNKHKDKENEDFVD 899
+ D V KII++HAQ + K K N+DFVD
Sbjct: 238 VLDLFVSKIIDEHAQYQEKGK-NKDFVD 264
Score = 57.0 bits (136), Expect(2) = 8e-81
Identities = 26/45 (57%), Positives = 34/45 (75%)
Frame = -3
Query: 798 IAGTDTSATSIEWTMSELLRHPHVMKKLKKELESVVGLGNMVDET 664
+ DTSAT I+W SEL+++P VMKKL+KELE VVG MV+E+
Sbjct: 293 VGSMDTSATVIDWAFSELIKNPRVMKKLQKELEEVVGKQRMVEES 337
Score = 143 bits (360), Expect = 2e-31
Identities = 65/112 (58%), Positives = 80/112 (71%)
Frame = -1
Query: 521 KSQIIVNAWAIMRDPNVWPDPDTFNPERFVGMDIDVRGLDFKLIPFGSGRRGCPGMXXXX 342
KS +I+N WAI RDP W D + F PERFVG DIDVRG DF+LIPFG+GRR CPGM
Sbjct: 381 KSHVIINVWAIGRDPKAWTDAEKFYPERFVGSDIDVRGRDFQLIPFGTGRRSCPGMQLGL 440
Query: 341 XXXXXXXXXXVHCFDWDLPDGMQPADLDMSEHFGIVTNRAKPLMAIPKYRLN 186
VHCFDW+LP+G+ P+++DMSE FG+V R+K L++IP YRLN
Sbjct: 441 TMVRLVLAQMVHCFDWELPNGILPSEVDMSEEFGLVLCRSKHLVSIPTYRLN 492
>ref|XP_003524281.1| PREDICTED: cytochrome P450 71A1-like [Glycine max]
Length = 536
Score = 268 bits (684), Expect(2) = 5e-80
Identities = 138/268 (51%), Positives = 181/268 (67%), Gaps = 5/268 (1%)
Frame = -2
Query: 1687 MVWIWSILSVLVFVYVVQKLLFGPKSNKKVPPGPRGLPILGHFHLLSSNPHHDLRNMARK 1508
M+WI L L ++++ + KK+PPGPRGLPILG H L NPH DL +A+K
Sbjct: 40 MIWIAIFLVSLAYMWLRR---ISKNKAKKLPPGPRGLPILGSLHKLGPNPHRDLHQLAQK 96
Query: 1507 HGPIMLIRFGYVPTVVITSPAAAELILKTHDHVFASRPRNDAAKYIGYEQRNLVSGPYGP 1328
+GP+M +R G+VPT+V++SP AAEL LKTHD VFASRP +AAKYI +EQRNL YG
Sbjct: 97 YGPVMHLRLGFVPTIVVSSPQAAELFLKTHDLVFASRPPLEAAKYISWEQRNLSFAEYGS 156
Query: 1327 YWRNMRKLVAWELLSSHRITHFQPMRNAEIRLMVDSLRRAEE---AVDLSKTISGLSGDM 1157
YWRN+RK+ ELLS +I F+ MR E+ LMV LR A + VDLS +S LS DM
Sbjct: 157 YWRNVRKMCTLELLSHTKINSFRSMREEELDLMVKLLREAAKDGAVVDLSAKVSTLSADM 216
Query: 1156 NCLMVFGRKY--SDLEQIGFKSVIAETMELAARFNLADYFPYIGVLDIQGINRRLKHLSK 983
+C MV G+KY DL++ GFK+V+ E M LAA N+ DY PYI LD+QG+ +R+K + K
Sbjct: 217 SCRMVLGKKYMDRDLDEKGFKAVMQEGMHLAATPNMGDYIPYIAALDLQGLTKRMKVVGK 276
Query: 982 IFDGIVEKIINDHAQNKHKDKENEDFVD 899
IFD EKII++H Q++ + +DFVD
Sbjct: 277 IFDDFFEKIIDEHLQSEKGEDRTKDFVD 304
Score = 58.5 bits (140), Expect(2) = 5e-80
Identities = 28/46 (60%), Positives = 40/46 (86%), Gaps = 1/46 (2%)
Frame = -3
Query: 798 IAGT-DTSATSIEWTMSELLRHPHVMKKLKKELESVVGLGNMVDET 664
+AG+ DTSAT+IEWT+SELL++P VMKK++ ELE+VVG+ V+E+
Sbjct: 332 LAGSMDTSATAIEWTLSELLKNPRVMKKVQMELETVVGMKRKVEES 377
Score = 135 bits (341), Expect = 3e-29
Identities = 65/112 (58%), Positives = 75/112 (66%)
Frame = -1
Query: 521 KSQIIVNAWAIMRDPNVWPDPDTFNPERFVGMDIDVRGLDFKLIPFGSGRRGCPGMXXXX 342
KS++IVNAWAIMRDP+ W + + F PERF G IDVRG DF+LIPFGSGRRGCPG+
Sbjct: 421 KSRVIVNAWAIMRDPSAWDEAEKFWPERFEGSSIDVRGRDFELIPFGSGRRGCPGLQLGL 480
Query: 341 XXXXXXXXXXVHCFDWDLPDGMQPADLDMSEHFGIVTNRAKPLMAIPKYRLN 186
VHCFDW LP + P DLDM E FG+ RA L AIP YRL+
Sbjct: 481 TVVRLTVAQIVHCFDWKLPKDILPDDLDMKEEFGLTMPRANHLHAIPTYRLS 532