BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Scutellaria22_contig00002726
         (1744 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   595   e-168
gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      592   e-166
gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   591   e-166
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   582   e-163
ref|XP_003531282.1| PREDICTED: cytochrome P450 716B2-like isofor...   560   e-157

>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
            vinifera]
          Length = 473

 Score =  595 bits (1535), Expect = e-168
 Identities = 281/445 (63%), Positives = 351/445 (78%)
 Frame = +3

Query: 180  NLPPGSYGWPILGETGEFLHAGLDGTPDKFVKERMEKYKSRVFKTSVMGEHMAVLCSTAG 359
            NLPPGS GWP +GET  FL +G DG P +F++ERM+KY S VFKTS++GE M V C  AG
Sbjct: 30   NLPPGSLGWPRIGETLVFLRSGEDGEPQRFIRERMDKYDSGVFKTSLLGEPMMVFCGPAG 89

Query: 360  NKFLFSNENKQVTVWWPSSVRKLLGPCLATSGGDEGKQMRKMVSYFVSPDAFTRLYIKTM 539
            NKFLF NENK V+VWWPSSVRKL   CL T+ GDE K+MR+M+  F++PDA  R Y++TM
Sbjct: 90   NKFLFGNENKLVSVWWPSSVRKLFRSCLVTATGDEAKRMRRMLLTFLNPDALKR-YVETM 148

Query: 540  DLVSQQHIKNHWQGQEKVKVFPIIKLYTFELACRLFMSLEDGEQISKLATLFNVFLKGII 719
            DLV+Q HI+  W+G E+++V+PIIKLYTFELACRLF S++D   ISKLA  FNVFLKGII
Sbjct: 149  DLVTQHHIRTQWEGNEEMQVYPIIKLYTFELACRLFASMDDPGHISKLAAHFNVFLKGII 208

Query: 720  SIPINFPGTRFYNAKRATSAIKTQLEKIVRQRRVALEEKRAAPSQDLLSHLLVTPDENGK 899
              PINFPGT+F  A +A +AI+ +L  +  +RRVALEEK A+ +QDLLSHLLVT D +G+
Sbjct: 209  DFPINFPGTKFXYATKAANAIREELRVMPMERRVALEEKTASATQDLLSHLLVTADASGR 268

Query: 900  FMSEAVIVNNILMLLFAGHDTSSASITMLMKNLAQLPHVYEKVLKEQNEIASRKEQGEFL 1079
            F++E  I++NIL+LLFAGHDT++++IT+L+K L +LP VY KVL+E+ +I   KE GE L
Sbjct: 269  FLTEMEIIDNILLLLFAGHDTTASAITLLIKYLGELPEVYAKVLREKIDITESKEPGELL 328

Query: 1080 QWGDLQKMRYSWTVVCEAMRLSPPIIGAFRQALTDISYAGYDIPKGWKLYWSSSFTHRDP 1259
            QW D QKMRYSW V  E MRLSPP  G+FR+AL D SYAGY+IPKGWKLYW +  T RDP
Sbjct: 329  QWKDXQKMRYSWHVASEVMRLSPPASGSFREALVDFSYAGYNIPKGWKLYWGTGSTQRDP 388

Query: 1260 SSYENCEVFDPSRFEEKGPAPYSYVPFGGGPRMCLGKEFARLEILIFLHNVIRRFKWDLM 1439
            + + N + FD SRFE  GPAP+SYVPFGGGPRMCLG+EFARL+IL+F+HN+++RF WDL+
Sbjct: 389  AFFRNPDNFDASRFEGAGPAPFSYVPFGGGPRMCLGQEFARLQILVFMHNIVKRFTWDLL 448

Query: 1440 IPDEKIAYDPMPTPVEGLPIFLHSH 1514
             PDEKI + PM  P+EGLPI LH H
Sbjct: 449  NPDEKIEHSPMLAPLEGLPIRLHPH 473


>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  592 bits (1525), Expect = e-166
 Identities = 278/453 (61%), Positives = 354/453 (78%), Gaps = 1/453 (0%)
 Frame = +3

Query: 159  KSTKRPLNLPPGSYGWPILGETGEFLHAGLDGTPDKFVKERMEKYKSR-VFKTSVMGEHM 335
            K  K  +NLPPGS+GWP LGET   L AG D  P++FV+ER++K+ S  VFKTS+ G+  
Sbjct: 22   KHQKTKINLPPGSFGWPFLGETLALLRAGWDSEPERFVRERIKKHGSPLVFKTSLFGDRF 81

Query: 336  AVLCSTAGNKFLFSNENKQVTVWWPSSVRKLLGPCLATSGGDEGKQMRKMVSYFVSPDAF 515
            AVLC  AGNKFLF NENK V  WWP  VRKL G  L T  GDE K MRKM+  ++ PDAF
Sbjct: 82   AVLCGPAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTIRGDEAKWMRKMLLSYLGPDAF 141

Query: 516  TRLYIKTMDLVSQQHIKNHWQGQEKVKVFPIIKLYTFELACRLFMSLEDGEQISKLATLF 695
               Y  TMD+V+++HI  HW+G+E+V VF  +KLY FELACRLFM+L+D   I+KL +LF
Sbjct: 142  ATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGSLF 201

Query: 696  NVFLKGIISIPINFPGTRFYNAKRATSAIKTQLEKIVRQRRVALEEKRAAPSQDLLSHLL 875
            N+FLKGII +PI+ PGTRFY++K+A +AI+ +L+K+++ R++ L+E + + SQDLLSHLL
Sbjct: 202  NIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKPSSSQDLLSHLL 261

Query: 876  VTPDENGKFMSEAVIVNNILMLLFAGHDTSSASITMLMKNLAQLPHVYEKVLKEQNEIAS 1055
             +PDENG F++E  IV+NIL+LLFAGHDTS+ SIT+LMK L +   VY+KVLKEQ EI+ 
Sbjct: 262  TSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKALGEHSDVYDKVLKEQLEISK 321

Query: 1056 RKEQGEFLQWGDLQKMRYSWTVVCEAMRLSPPIIGAFRQALTDISYAGYDIPKGWKLYWS 1235
             KE GE L+W D+QKM+YSW+VVCE MRL+PP+IGA+R+AL DI YAGY IPKGWKL+WS
Sbjct: 322  GKEAGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYREALVDIDYAGYTIPKGWKLHWS 381

Query: 1236 SSFTHRDPSSYENCEVFDPSRFEEKGPAPYSYVPFGGGPRMCLGKEFARLEILIFLHNVI 1415
            +  T RD +++E+   FDPSRFE  GP P+++VPFGGGPRMCLGKEFARLE+L FLHN++
Sbjct: 382  AVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFARLEVLAFLHNIV 441

Query: 1416 RRFKWDLMIPDEKIAYDPMPTPVEGLPIFLHSH 1514
              FKWDL+IPDEKI YDP+ TP +GLPI LH H
Sbjct: 442  INFKWDLLIPDEKIEYDPIATPAKGLPIRLHPH 474


>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
            gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
            13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  591 bits (1524), Expect = e-166
 Identities = 277/453 (61%), Positives = 353/453 (77%), Gaps = 1/453 (0%)
 Frame = +3

Query: 159  KSTKRPLNLPPGSYGWPILGETGEFLHAGLDGTPDKFVKERMEKYKSR-VFKTSVMGEHM 335
            K  K  +NLPPGS+GWP LGET   L AG D  P++FV+ER++K+ S  VFKTS+ G+  
Sbjct: 22   KHQKTKINLPPGSFGWPFLGETLALLRAGWDSEPERFVRERIKKHGSPLVFKTSLFGDRF 81

Query: 336  AVLCSTAGNKFLFSNENKQVTVWWPSSVRKLLGPCLATSGGDEGKQMRKMVSYFVSPDAF 515
            AVLC  AGNKFLF NENK V  WWP  VRKL G  L T  GDE K MRKM+  ++ PDAF
Sbjct: 82   AVLCGPAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTIRGDEAKWMRKMLLSYLGPDAF 141

Query: 516  TRLYIKTMDLVSQQHIKNHWQGQEKVKVFPIIKLYTFELACRLFMSLEDGEQISKLATLF 695
               Y  TMD+V+++HI  HW+G+E+V VF  +KLY FELACRLFM+L+D   I+KL +LF
Sbjct: 142  ATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGSLF 201

Query: 696  NVFLKGIISIPINFPGTRFYNAKRATSAIKTQLEKIVRQRRVALEEKRAAPSQDLLSHLL 875
            N+FLKGII +PI+ PGTRFY++K+A +AI+ +L+K+++ R++ L+E +A+ SQDLLSHLL
Sbjct: 202  NIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKASSSQDLLSHLL 261

Query: 876  VTPDENGKFMSEAVIVNNILMLLFAGHDTSSASITMLMKNLAQLPHVYEKVLKEQNEIAS 1055
             +PDENG F++E  IV+NIL+LLFAGHDTS+ SIT+LMK L +   VY+KVLKEQ EI+ 
Sbjct: 262  TSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKTLGEHSDVYDKVLKEQLEISK 321

Query: 1056 RKEQGEFLQWGDLQKMRYSWTVVCEAMRLSPPIIGAFRQALTDISYAGYDIPKGWKLYWS 1235
             KE  E L+W D+QKM+YSW+V+CE MRL+PP+IG +R+AL DI YAGY IPKGWKL+WS
Sbjct: 322  TKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYREALVDIDYAGYTIPKGWKLHWS 381

Query: 1236 SSFTHRDPSSYENCEVFDPSRFEEKGPAPYSYVPFGGGPRMCLGKEFARLEILIFLHNVI 1415
            +  T RD +++E+   FDPSRFE  GP P+++VPFGGGPRMCLGKEFARLE+L FLHN++
Sbjct: 382  AVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFARLEVLAFLHNIV 441

Query: 1416 RRFKWDLMIPDEKIAYDPMPTPVEGLPIFLHSH 1514
              FKWDL+IPDEKI YDPM TP +GLPI LH H
Sbjct: 442  TNFKWDLLIPDEKIEYDPMATPAKGLPIRLHPH 474


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
            annua]
          Length = 480

 Score =  582 bits (1499), Expect = e-163
 Identities = 272/460 (59%), Positives = 348/460 (75%), Gaps = 2/460 (0%)
 Frame = +3

Query: 141  FMKITRKSTKRPLNLPPGSYGWPILGETGEFLHAGLDGTPDKFVKERMEKYKSR-VFKTS 317
            F  + R ++    NLPPGS+GWP +GET  ++ +   G P++F KER+EKY S  VFKTS
Sbjct: 19   FFLVHRHNSTTTKNLPPGSFGWPFIGETLAYIRSKRGGDPERFTKERIEKYGSTLVFKTS 78

Query: 318  VMGEHMAVLCSTAGNKFLFSNENKQVTVWWPSSVRKLLGPCLATSGGDEGKQMRKMVSYF 497
            V GE MAV C   GNKFLF NENK V  WWP+SVR L   CL T  GDE K +RKM+  +
Sbjct: 79   VAGERMAVFCGPEGNKFLFGNENKLVASWWPNSVRILFEKCLITIRGDEAKWLRKMMFAY 138

Query: 498  VSPDAFTRLYIKTMDLVSQQHIKNHWQGQEKVKVFPIIKLYTFELACRLFMSLEDGEQIS 677
            + PDA +  Y  TM++V++ HI+NHWQG+ ++KVF  ++ Y FELACRLF+SL+D + ++
Sbjct: 139  LGPDALSNRYTGTMEVVTRLHIQNHWQGKSELKVFETVRPYLFELACRLFLSLDDPKHVA 198

Query: 678  KLATLFNVFLKGIISIPINFPGTRFYNAKRATSAIKTQLEKIVRQRRVAL-EEKRAAPSQ 854
            +L TLFN FLKG+  +PIN PGTRFY AKRA +AIK QL  I++QRR AL +E +++  +
Sbjct: 199  ELGTLFNTFLKGLTELPINIPGTRFYRAKRAANAIKKQLIVIIKQRRQALKQEDQSSSFE 258

Query: 855  DLLSHLLVTPDENGKFMSEAVIVNNILMLLFAGHDTSSASITMLMKNLAQLPHVYEKVLK 1034
            DLLSHLLV+ DENG+F+SEA I NN+L+LLFAGHDTS+ SIT+LMK+LA+ P VY+ VLK
Sbjct: 259  DLLSHLLVSSDENGRFLSEAEIANNVLLLLFAGHDTSAVSITLLMKSLAEHPQVYDNVLK 318

Query: 1035 EQNEIASRKEQGEFLQWGDLQKMRYSWTVVCEAMRLSPPIIGAFRQALTDISYAGYDIPK 1214
            EQ  I   K  GE L W D+QKMRYSW VVCE MRL PP++G+FR+AL D  YAGY IPK
Sbjct: 319  EQLGILEAKAPGEMLNWEDIQKMRYSWYVVCEVMRLIPPVVGSFREALVDFEYAGYTIPK 378

Query: 1215 GWKLYWSSSFTHRDPSSYENCEVFDPSRFEEKGPAPYSYVPFGGGPRMCLGKEFARLEIL 1394
            GWK+ WS+  TH++ +++ N   FDPSRFE  GP P++YVPFGGGPRMCLGKE AR+ IL
Sbjct: 379  GWKIIWSAVMTHKEENNFPNATKFDPSRFEGAGPTPFTYVPFGGGPRMCLGKELARVRIL 438

Query: 1395 IFLHNVIRRFKWDLMIPDEKIAYDPMPTPVEGLPIFLHSH 1514
            +FLHN++ +FKWDL+IPDEKI YDP+ TPV+GLP+ LH H
Sbjct: 439  VFLHNIMTKFKWDLLIPDEKIGYDPLATPVKGLPVRLHPH 478


>ref|XP_003531282.1| PREDICTED: cytochrome P450 716B2-like isoform 1 [Glycine max]
          Length = 481

 Score =  560 bits (1443), Expect = e-157
 Identities = 260/444 (58%), Positives = 344/444 (77%)
 Frame = +3

Query: 177  LNLPPGSYGWPILGETGEFLHAGLDGTPDKFVKERMEKYKSRVFKTSVMGEHMAVLCSTA 356
            LNLPPG  G PI+GET EFL    +G   +F++ER+EKY +RVFKTS+ G+ + V C  A
Sbjct: 35   LNLPPGRLGCPIVGETLEFLRTMNEGNVLRFIQERVEKYDARVFKTSMFGDPVVVFCGPA 94

Query: 357  GNKFLFSNENKQVTVWWPSSVRKLLGPCLATSGGDEGKQMRKMVSYFVSPDAFTRLYIKT 536
            GNKFLFSNENK V VWWPSSVRKLL   L    GDE K +R+++  F++ +   R Y+  
Sbjct: 95   GNKFLFSNENKNVQVWWPSSVRKLLRLSLVNKVGDEAKMVRRLLMSFLNAETL-RNYLPK 153

Query: 537  MDLVSQQHIKNHWQGQEKVKVFPIIKLYTFELACRLFMSLEDGEQISKLATLFNVFLKGI 716
            MD ++Q+HI  +W+G+E+V V+PI++LYTFELAC LF+S+ED + ISKL+  F+ FLKGI
Sbjct: 154  MDSIAQRHIDTYWEGKEQVLVYPIVQLYTFELACCLFLSIEDSDHISKLSLKFDEFLKGI 213

Query: 717  ISIPINFPGTRFYNAKRATSAIKTQLEKIVRQRRVALEEKRAAPSQDLLSHLLVTPDENG 896
            I +P+N PGTRF+ A +A   I+ ++E I+++R+V LEEKRA+P+QDLLSH+LVT D NG
Sbjct: 214  IGLPLNIPGTRFHRAMKAADVIRNEIEMILKKRKVDLEEKRASPTQDLLSHMLVTSDPNG 273

Query: 897  KFMSEAVIVNNILMLLFAGHDTSSASITMLMKNLAQLPHVYEKVLKEQNEIASRKEQGEF 1076
            +FM+E  I++NIL+LLFAGHD+S + ++++MK L QLP VYE VLKEQ EI+  KE G+ 
Sbjct: 274  RFMTEMEIIDNILLLLFAGHDSSRSVLSLVMKYLGQLPQVYEHVLKEQLEISQGKEAGQL 333

Query: 1077 LQWGDLQKMRYSWTVVCEAMRLSPPIIGAFRQALTDISYAGYDIPKGWKLYWSSSFTHRD 1256
            LQW D+QKM+YSW V  E MRLSPP+ GA+R+A+ D +Y  Y+IPKGWKL+W++  +H D
Sbjct: 334  LQWEDVQKMKYSWNVASEVMRLSPPVSGAYREAIKDFTYGDYNIPKGWKLHWNTGSSHED 393

Query: 1257 PSSYENCEVFDPSRFEEKGPAPYSYVPFGGGPRMCLGKEFARLEILIFLHNVIRRFKWDL 1436
            P+ + N E FD SRFE  GP P+SYVPFGGGPRMCLG+EFARLEIL+F+HN+++RFKWDL
Sbjct: 394  PALFSNPETFDASRFEGAGPTPFSYVPFGGGPRMCLGQEFARLEILVFMHNIVKRFKWDL 453

Query: 1437 MIPDEKIAYDPMPTPVEGLPIFLH 1508
            +IPDEK  YDP+  PV+GL I LH
Sbjct: 454  VIPDEKFKYDPLLEPVKGLAIRLH 477