BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Scutellaria22_contig00001727
         (2193 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   874   0.0  
dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]    872   0.0  
sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,...   870   0.0  
gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus lon...   867   0.0  
gb|AFP93564.1| GCS [Cestrum nocturnum]                                865   0.0  

>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
            communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
            ligase, chloroplast precursor, putative [Ricinus
            communis]
          Length = 526

 Score =  874 bits (2258), Expect = 0.0
 Identities = 424/523 (81%), Positives = 467/523 (89%), Gaps = 4/523 (0%)
 Frame = -3

Query: 1993 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNLCSPSWSSLKVNKSW 1814
            M ++S    +  + ++I + K  +N V    N++E+ + KE      S S +S K+ +  
Sbjct: 1    MPVISQAGPSCYVPTDITRCKTGQNAVLGVFNSIETSKLKETCVRFSSLSCNSTKIPQLP 60

Query: 1813 SS----VGQRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEK 1646
            SS    V  RRG ++ VAASPP EDAVIATEPLTKEDLV YLASGCKPK+ WRIGTEHEK
Sbjct: 61   SSENSWVRSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEK 120

Query: 1645 FGFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSG 1466
            FGFE+GTLRPMKYEQI+ELLNGI+ERFDW+K+MEG+YIIGLKQGKQSISLEPGGQFELSG
Sbjct: 121  FGFELGTLRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSG 180

Query: 1465 APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYM 1286
            APLETLHQTCAEVNSHLYQVKAV EEMGIGFLGIGFQPKW  +DIP+MPKGRYEIMRNYM
Sbjct: 181  APLETLHQTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYM 240

Query: 1285 PKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDY 1106
            PKVG+LGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN Y
Sbjct: 241  PKVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGY 300

Query: 1105 LSMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD 926
            LSMRSQIWTDTD +R GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC G++FRD
Sbjct: 301  LSMRSQIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRD 360

Query: 925  FMAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL 746
            F+AGKL  +PGE PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+L
Sbjct: 361  FLAGKLPCIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLL 420

Query: 745  YDEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERR 566
            YDE+SLQNVLDMTADWTPEER+MLRNKVPK+GLKTPFRDGLLKHVA++V+  AKDGLERR
Sbjct: 421  YDEISLQNVLDMTADWTPEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERR 480

Query: 565  GFKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 437
            G+KE GFLNEVTEVV+TGVTPAE+LLELY+GKWGQSVDPVFEE
Sbjct: 481  GYKEIGFLNEVTEVVRTGVTPAERLLELYNGKWGQSVDPVFEE 523


>dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]
          Length = 523

 Score =  872 bits (2253), Expect = 0.0
 Identities = 429/525 (81%), Positives = 473/525 (90%), Gaps = 6/525 (1%)
 Frame = -3

Query: 1993 MALMSHPASAQSIHSEIIQLK-GRRNGVNSSANTME-SGRSKEVLFNLCSPSWSSLKVNK 1820
            M LMS  + +  IH+EI+Q K G  + +N ++NT     ++ +V F     S + LK  +
Sbjct: 1    MVLMSQASPSHGIHAEILQSKSGYSSLLNGASNTNAFRHQTSKVAF-----SRNYLKYTQ 55

Query: 1819 SW--SSVGQ--RRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEH 1652
            +    +VG   +RG ++ VAASPPTEDAV+ATEPLTKEDLV YLASGCKPK+NWRIGTEH
Sbjct: 56   AMHVDAVGGNFKRGNKVIVAASPPTEDAVVATEPLTKEDLVGYLASGCKPKENWRIGTEH 115

Query: 1651 EKFGFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFEL 1472
            EKFGF++ TLRPM YEQI+ LLN ISERFDW+K+MEG+ IIGLKQGKQSISLEPGGQFEL
Sbjct: 116  EKFGFDLKTLRPMTYEQIAHLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFEL 175

Query: 1471 SGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRN 1292
            SGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGF+GIGFQPKW R+DIP+MPKGRYEIMRN
Sbjct: 176  SGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFIGIGFQPKWERKDIPIMPKGRYEIMRN 235

Query: 1291 YMPKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPN 1112
            YMPKVGSLGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN
Sbjct: 236  YMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPN 295

Query: 1111 DYLSMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSF 932
             YLSMRSQIWTDTDN+R+GMLPFVFDDSFGFEQYV+YALDVPMYFVYRKKKYIDCAGLSF
Sbjct: 296  GYLSMRSQIWTDTDNDRSGMLPFVFDDSFGFEQYVEYALDVPMYFVYRKKKYIDCAGLSF 355

Query: 931  RDFMAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 752
            RDF+AGKL  +PGE+PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG
Sbjct: 356  RDFLAGKLPPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG 415

Query: 751  ILYDEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLE 572
            +LYD++SLQNVLDMTADWT EERQMLRNKVP +GLKTPFRDGLLKHVA+EV+ FAKDGLE
Sbjct: 416  VLYDDISLQNVLDMTADWTQEERQMLRNKVPVAGLKTPFRDGLLKHVAEEVLKFAKDGLE 475

Query: 571  RRGFKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 437
            RRG+KETGFLNEV EVV+TG+TPAEKLLELYHGKWGQSVDPVFEE
Sbjct: 476  RRGYKETGFLNEVAEVVRTGLTPAEKLLELYHGKWGQSVDPVFEE 520


>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
            Full=Gamma-ECS; Short=GCS; AltName:
            Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
            gi|111380512|gb|ABD98695.2| chloroplast
            gamma-glutamylcysteine synthetase [Nicotiana tabacum]
          Length = 522

 Score =  870 bits (2247), Expect = 0.0
 Identities = 431/522 (82%), Positives = 463/522 (88%), Gaps = 3/522 (0%)
 Frame = -3

Query: 1993 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNLCSPSWSSLKVNKSW 1814
            MALMS   S+  I+SE ++     +G +S  + ME  + K++ F   S   SS  +   +
Sbjct: 1    MALMSQAGSSHCIYSEKMKCI---SGHSSITSNMEMLKMKDICFGNISSRNSSKPMQGIY 57

Query: 1813 SS---VGQRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEKF 1643
                 V +RRG    VAASPPTEDAV+A EPLTKEDLV YLASGCK K+ WRIGTEHEKF
Sbjct: 58   LDRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKF 117

Query: 1642 GFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSGA 1463
            GFE GTLRPMKYEQI+ELLNGI+ERFDW+K+MEG+ IIGLKQGKQSISLEPGGQFELSGA
Sbjct: 118  GFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGA 177

Query: 1462 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYMP 1283
            PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKW  +DIPVMPKGRYEIMRNYMP
Sbjct: 178  PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMP 237

Query: 1282 KVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDYL 1103
            KVGSLGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN YL
Sbjct: 238  KVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 297

Query: 1102 SMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 923
            SMRS IWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAG+SFRDF
Sbjct: 298  SMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDF 357

Query: 922  MAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 743
            M GKLS +PG++PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY
Sbjct: 358  MNGKLSPIPGDYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 417

Query: 742  DEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERRG 563
            DEVSLQ VLDMT+DWT EER+MLRNKVP SGLKTPFRDGLLKHVAQ+VV  AK+GLERRG
Sbjct: 418  DEVSLQTVLDMTSDWTAEEREMLRNKVPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRG 477

Query: 562  FKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 437
            +KETGFLNEVTEVV+TGVTPAEKLLELYHGKWG+SVDPVFEE
Sbjct: 478  YKETGFLNEVTEVVRTGVTPAEKLLELYHGKWGRSVDPVFEE 519


>gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus longan]
          Length = 511

 Score =  867 bits (2240), Expect = 0.0
 Identities = 430/519 (82%), Positives = 458/519 (88%)
 Frame = -3

Query: 1993 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNLCSPSWSSLKVNKSW 1814
            MAL+S   +   + SEI    G R GV    N +E+ R KE        S+SSL     +
Sbjct: 1    MALVSQGGATYRVRSEITP--GHR-GVFGLVNNVEASRVKESCV-----SFSSLSC---Y 49

Query: 1813 SSVGQRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEKFGFE 1634
            S+ G RRG R  VAASPPT+DAVIA EPLTKEDLV YLASGCKP++ WRIGTEHEKFGFE
Sbjct: 50   STKGSRRGYRAIVAASPPTDDAVIAAEPLTKEDLVGYLASGCKPREKWRIGTEHEKFGFE 109

Query: 1633 IGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSGAPLE 1454
            IGTLRPMKYEQI+ELLN I+ERFDW+KIME   IIGLKQGKQSISLEPGGQFELSGAPLE
Sbjct: 110  IGTLRPMKYEQIAELLNSIAERFDWEKIMEDNNIIGLKQGKQSISLEPGGQFELSGAPLE 169

Query: 1453 TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYMPKVG 1274
            TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW  +DIPVMPKGRYEIM+NYMPKVG
Sbjct: 170  TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMKNYMPKVG 229

Query: 1273 SLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDYLSMR 1094
            SLGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPFTEGKPN YLSMR
Sbjct: 230  SLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMR 289

Query: 1093 SQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFMAG 914
            SQIWTDTDNNR GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC G+SFRDF+ G
Sbjct: 290  SQIWTDTDNNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFLVG 349

Query: 913  KLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEV 734
            KL  LPGE PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDEV
Sbjct: 350  KLPCLPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEV 409

Query: 733  SLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERRGFKE 554
            SLQN LD+TADWT EERQMLRNKVPK+GLKTPFRDGLL+HVAQ+++  AKDGLERRGFKE
Sbjct: 410  SLQNALDLTADWTSEERQMLRNKVPKTGLKTPFRDGLLRHVAQDILKLAKDGLERRGFKE 469

Query: 553  TGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 437
            +GFLN V EVV+TGVTPAEKLLE+YHGKWGQSVDPVFEE
Sbjct: 470  SGFLNAVAEVVRTGVTPAEKLLEMYHGKWGQSVDPVFEE 508


>gb|AFP93564.1| GCS [Cestrum nocturnum]
          Length = 518

 Score =  865 bits (2236), Expect = 0.0
 Identities = 432/523 (82%), Positives = 461/523 (88%), Gaps = 4/523 (0%)
 Frame = -3

Query: 1993 MALMSHPASAQSIHSEIIQLKGRRNGVNSSANTMESGRSKEVLFNL-CSPSWSS-LKVNK 1820
            MALMS   S+  IH E I+ KG  N        ME  + KE+ F +  SP  SS  K   
Sbjct: 1    MALMSQAGSSHCIHPEKIRCKGGHN--------MEIFKLKEICFGVHISPRNSSNAKQGI 52

Query: 1819 SWSSVG--QRRGCRLTVAASPPTEDAVIATEPLTKEDLVDYLASGCKPKDNWRIGTEHEK 1646
                VG  +RRG    VAASPPT+DAV+A EPLTKEDLV YLASGCK K+ WRIGTEHEK
Sbjct: 53   CLRRVGAERRRGRLSIVAASPPTDDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEK 112

Query: 1645 FGFEIGTLRPMKYEQISELLNGISERFDWDKIMEGEYIIGLKQGKQSISLEPGGQFELSG 1466
            FGFE GTLRPM YEQI+ELL G+SERFDW+K+MEG+ IIGLKQGKQSISLEPGGQFELSG
Sbjct: 113  FGFEFGTLRPMNYEQIAELLYGVSERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSG 172

Query: 1465 APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPVMPKGRYEIMRNYM 1286
            APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW  +DIPVMPKGRYEIMRNYM
Sbjct: 173  APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYM 232

Query: 1285 PKVGSLGLDMMFRTCTVQVNLDFDSEADMIRKFRAGLALQPIATALFANSPFTEGKPNDY 1106
            PKVG+LGLDMMFRTCTVQVNLDF SEADMIRKFRAGLALQPIATALFANSPF EGKPN Y
Sbjct: 233  PKVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFKEGKPNGY 292

Query: 1105 LSMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD 926
            LSMRS IWTDTDNNR+GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD
Sbjct: 293  LSMRSHIWTDTDNNRSGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRD 352

Query: 925  FMAGKLSSLPGEFPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL 746
            F+ GKL+ +PGE+PTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL
Sbjct: 353  FLNGKLAPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGIL 412

Query: 745  YDEVSLQNVLDMTADWTPEERQMLRNKVPKSGLKTPFRDGLLKHVAQEVVNFAKDGLERR 566
            YDE SLQ+VLDMTADWT EER+MLRNKVPKSGLKTPFRDGLLKHVAQ+V+  AK+GLERR
Sbjct: 413  YDEASLQSVLDMTADWTAEEREMLRNKVPKSGLKTPFRDGLLKHVAQDVMKLAKEGLERR 472

Query: 565  GFKETGFLNEVTEVVKTGVTPAEKLLELYHGKWGQSVDPVFEE 437
            G+KETGFLNEVTEVV+TGVTPAEKLL+LYHGKWGQSVDPVFEE
Sbjct: 473  GYKETGFLNEVTEVVRTGVTPAEKLLDLYHGKWGQSVDPVFEE 515