BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rheum21_contig00022327
         (647 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola...   289   4e-76
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|...   289   4e-76
gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]                 288   9e-76
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ...   286   4e-75
gb|EXB39337.1| Isoamylase 2 [Morus notabilis]                         285   8e-75
gb|EMJ02949.1| hypothetical protein PRUPE_ppa001199mg [Prunus pe...   282   5e-74
ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   281   2e-73
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   280   2e-73
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu...   279   4e-73
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ...   276   5e-72
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr...   276   5e-72
gb|ESW21083.1| hypothetical protein PHAVU_005G040300g [Phaseolus...   275   6e-72
gb|AAY89379.1| isoamylase isoform 2 [Nicotiana langsdorffii x Ni...   275   1e-71
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   274   1e-71
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   274   2e-71
ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Caps...   273   3e-71
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   272   7e-71
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   272   7e-71
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ...   269   6e-70
ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata sub...   269   6e-70

>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
          Length = 878

 Score =  289 bits (740), Expect = 4e-76
 Identities = 143/234 (61%), Positives = 173/234 (73%), Gaps = 19/234 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +F   G+ NFA FSR+A SV+LCLY D + EKP+LEI+LDPY+NR+GDIWHA+LD +  +
Sbjct: 227 SFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPF 286

Query: 182 MNYGYRIK-------NKVLLDPYAKVISK------------SGLAKLSRIPAFDWGGDMP 304
             YGYR K         VLLDPYAKVI +              L +L   P +DW GD+P
Sbjct: 287 KTYGYRCKATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVP 346

Query: 305 PSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPF 484
           PSLPME+L++YRLNV +FTKDKSSKLP   AGTF+G++EK  HFK LGVNA+LLEP+FPF
Sbjct: 347 PSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPF 406

Query: 485 DERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           DE+KG Y+P HFFSP N+YGP GD  SAI SMK+MVK +HANGIEV LEVVFTH
Sbjct: 407 DEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTH 460


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
           isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  289 bits (740), Expect = 4e-76
 Identities = 143/234 (61%), Positives = 173/234 (73%), Gaps = 19/234 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +F   G+ NFA FSR+A SV+LCLY D + EKP+LEI+LDPY+NR+GDIWHA+LD +  +
Sbjct: 227 SFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPF 286

Query: 182 MNYGYRIK-------NKVLLDPYAKVISK------------SGLAKLSRIPAFDWGGDMP 304
             YGYR K         VLLDPYAKVI +              L +L   P +DW GD+P
Sbjct: 287 KTYGYRCKATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVP 346

Query: 305 PSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPF 484
           PSLPME+L++YRLNV +FTKDKSSKLP   AGTF+G++EK  HFK LGVNA+LLEP+FPF
Sbjct: 347 PSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPF 406

Query: 485 DERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           DE+KG Y+P HFFSP N+YGP GD  SAI SMK+MVK +HANGIEV LEVVFTH
Sbjct: 407 DEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTH 460


>gb|EOX94834.1| Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  288 bits (737), Expect = 9e-76
 Identities = 143/235 (60%), Positives = 176/235 (74%), Gaps = 20/235 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FS+ G+ NFA +SRNA+S++LCLY D+ +EKPALE++LDP+VNRTGDIWHAS++ +  +
Sbjct: 226 SFSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTF 285

Query: 182 MNYGYRIKN--------KVLLDPYAKVISKS------------GLAKLSRIPAFDWGGDM 301
           + YGYR K         +VLLDPYA++I  S             L +L + PAFDW  D+
Sbjct: 286 VRYGYRCKGDTDAFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSSDV 345

Query: 302 PPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFP 481
            P+LPME+LVVYRLNVL FT+DKSSKLP    GTFAGVTEK+QH K LGVNAVLLEP+F 
Sbjct: 346 CPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPIFT 405

Query: 482 FDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           FDE+KG Y+P HFFSP NLYGP     SAI S+KEMVK +HANG+EV LEVVFTH
Sbjct: 406 FDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTH 460


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
           vesca]
          Length = 868

 Score =  286 bits (732), Expect = 4e-75
 Identities = 143/237 (60%), Positives = 177/237 (74%), Gaps = 22/237 (9%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           ++SS G+ NFA FSRNA+SV+LCLY D TA++PALE++LDPYVNR+GDIWHAS +SA  +
Sbjct: 225 SYSSDGSINFAIFSRNAESVVLCLYDDPTAQEPALELDLDPYVNRSGDIWHASFESAWTF 284

Query: 182 MNYGYRIKN------------KVLLDPYAKVISKS-------GLAKLSRI---PAFDWGG 295
           ++YGYR K              VLLDPYAKVI +S       GL  L R+   P FDW G
Sbjct: 285 LSYGYRFKGTSLRNTNSLDEGNVLLDPYAKVIDESIANNRGTGLKLLGRLCEEPVFDWDG 344

Query: 296 DMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPV 475
           D+ P LP+E+LVVYRLN+ +FT+ KSSKLP   AGTF+G+T+KL HF+ LGVNA+LLEP+
Sbjct: 345 DVRPLLPIEKLVVYRLNLKRFTEHKSSKLPTNVAGTFSGLTQKLDHFQDLGVNAILLEPI 404

Query: 476 FPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           FPFDE KG Y+P HFFSP+N +GP G   ++I SMKEMVK  HANGIEV LEV+FTH
Sbjct: 405 FPFDEEKGPYFPCHFFSPMNCFGPSGGPIASINSMKEMVKEFHANGIEVILEVIFTH 461


>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
          Length = 825

 Score =  285 bits (729), Expect = 8e-75
 Identities = 139/236 (58%), Positives = 179/236 (75%), Gaps = 21/236 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FS  G+ NF+ FSR+A+SV+LCLY D+TA+ PALE++LDPYVNRTGDIWHAS +S+S +
Sbjct: 186 SFSRDGSLNFSIFSRSAESVVLCLYDDTTADNPALELDLDPYVNRTGDIWHASFESSSTF 245

Query: 182 MNYGYRIKN-----------KVLLDPYAKVISKSG----------LAKLSRIPAFDWGGD 298
           ++YGYR+K            +++LDPYAK+I KS           L ++S+ P FDW GD
Sbjct: 246 VSYGYRLKGSRLKGKKDEDARIVLDPYAKIIGKSTSSDHGIGPKYLGRISKEPGFDWSGD 305

Query: 299 MPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVF 478
           + P+LP+E+L VYRLNV+ FT+ KSS+L P  AGTF+G+ EKL+HF  LGVNAVLLEP+F
Sbjct: 306 IRPNLPIEKLTVYRLNVMHFTEHKSSQLSPDIAGTFSGLIEKLEHFTDLGVNAVLLEPIF 365

Query: 479 PFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           PFDE+KG  +P HFFSP +LYGP G   SAI +MKEMVK +HA+GIEV LEVVFTH
Sbjct: 366 PFDEQKGPCFPCHFFSPTSLYGPSGGAVSAINTMKEMVKKLHASGIEVLLEVVFTH 421


>gb|EMJ02949.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score =  282 bits (722), Expect = 5e-74
 Identities = 144/237 (60%), Positives = 175/237 (73%), Gaps = 23/237 (9%)
 Frame = +2

Query: 5   FSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGYM 184
           FS+ G+ NFA FSRNA+SV LCLY ++TAEKPALE++LDPYVNR+GDIWH S  SA  ++
Sbjct: 241 FSNDGSMNFAIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFV 300

Query: 185 NYGYRIKNK-------------VLLDPYAKVISKS-------GLAKLSRI---PAFDWGG 295
           +YGY+ K               VLLDPYAKVI+KS       GL  L R+   PAFDW G
Sbjct: 301 SYGYKFKGNLLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGLKYLGRLCEEPAFDWAG 360

Query: 296 DMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPV 475
           D+ P L ME+LVVYRLNV +FT+ KSS+LP    G+F+G+TEKL+HFK LGVNAVLLEP+
Sbjct: 361 DVRPDLSMEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPI 420

Query: 476 FPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           FPFDE+KG Y+P HFFSP++ +GP     SA+ SMKEMV+  HANGIEV LEVVFTH
Sbjct: 421 FPFDEQKGPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTH 477


>ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
           [Solanum lycopersicum]
          Length = 878

 Score =  281 bits (718), Expect = 2e-73
 Identities = 138/234 (58%), Positives = 167/234 (71%), Gaps = 19/234 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +F   G  NFA +SR+A  V+LCLY D + EKP+LEI+LDPY+N++GDIWHA+LD +  +
Sbjct: 227 SFQPDGYVNFALYSRSAKGVVLCLYDDISVEKPSLEIDLDPYINQSGDIWHAALDCSLPF 286

Query: 182 MNYGYRIK-------NKVLLDPYAKVISK------------SGLAKLSRIPAFDWGGDMP 304
             YGYR K         VLLDPYAKVI                L +L   P +DW GD+P
Sbjct: 287 KTYGYRFKAATSGKGELVLLDPYAKVIRSVIPRQGGSEIRPKYLGELCLEPGYDWSGDVP 346

Query: 305 PSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPF 484
           PSLPME+L++YR+NV  FTKDKSSKLP   AGTF+G++EK  H K LGVNA+LLEP+FPF
Sbjct: 347 PSLPMEKLIIYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPF 406

Query: 485 DERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           DE+KG YYP HFFSP N+YGP GD  S I SMK+MVK +HANGIEV LEVVFTH
Sbjct: 407 DEQKGPYYPWHFFSPGNMYGPSGDPLSVIKSMKDMVKKLHANGIEVFLEVVFTH 460


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
           isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  280 bits (717), Expect = 2e-73
 Identities = 141/239 (58%), Positives = 173/239 (72%), Gaps = 24/239 (10%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FS+ G+ NFAFFSRN + ++LCLY DST +KPALE++LDPYVNRTGD+WHASL+ A  +
Sbjct: 229 SFSTDGSMNFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTF 288

Query: 182 MNYGYRIKNK-------------VLLDPYAKVISKSG-----------LAKLSRIPAFDW 289
            +YGYR K               VLLDPYA+VI+ S            L +L   PAF+W
Sbjct: 289 TSYGYRCKGAILQGNTSKVDMECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEW 348

Query: 290 GGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLE 469
           G D+ P+L ME+L+VYRLNV +FT+ KS KL    AGTFAG+ EK+ HF++LGVNAVLLE
Sbjct: 349 GSDIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLE 408

Query: 470 PVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           P+FPFDE+KG Y+P HFFSP N+YGP G   SAI SMKEMVK +HAN IEV LEVVFTH
Sbjct: 409 PIFPFDEQKGPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTH 467


>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
           gi|550345605|gb|ERP64619.1| hypothetical protein
           POPTR_0002s22530g [Populus trichocarpa]
          Length = 857

 Score =  279 bits (714), Expect = 4e-73
 Identities = 141/239 (58%), Positives = 173/239 (72%), Gaps = 24/239 (10%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FS+ G+ NFAFFSRNA   +LCLY DST+ KPALE++LDPYVNR+GDIWHASL+ A  +
Sbjct: 217 SFSTDGSMNFAFFSRNAAGCVLCLYDDSTSGKPALELDLDPYVNRSGDIWHASLEGAWTF 276

Query: 182 MNYGYRIKN-------------KVLLDPYAKVISKSG-----------LAKLSRIPAFDW 289
           ++YGYR K              +VLLDPY+K+I  S            L +L + P FDW
Sbjct: 277 LSYGYRCKGAALQSDADKFDAGRVLLDPYSKIIINSVTDNVSGLLPKYLGRLCKEPVFDW 336

Query: 290 GGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLE 469
             D+PP L ME+LVVYR+NV++FTKD SS++    AGTFAG+ EKL HFK+LGVNAVLLE
Sbjct: 337 SDDVPPHLEMEKLVVYRVNVMRFTKDNSSQISSDAAGTFAGLIEKLSHFKNLGVNAVLLE 396

Query: 470 PVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
            +FPFDE+KG Y+P HFFSP N+YGP     +AI SMKEMVK +HANGIEV LEVVFTH
Sbjct: 397 SIFPFDEQKGPYFPCHFFSPSNVYGPSNGSVAAISSMKEMVKRLHANGIEVLLEVVFTH 455


>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
           sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
           isoamylase 2, chloroplastic-like isoform X2 [Citrus
           sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
           isoamylase 2, chloroplastic-like isoform X3 [Citrus
           sinensis]
          Length = 889

 Score =  276 bits (705), Expect = 5e-72
 Identities = 139/239 (58%), Positives = 172/239 (71%), Gaps = 25/239 (10%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FS  G+ NFA FSRNA  V+LCLY D+TA++PALE++LDPY+NR+GDIWHAS++S   +
Sbjct: 244 SFSIDGSLNFAIFSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMESTWNF 303

Query: 182 MNYGYRIKNK-------------VLLDPYAKVISKS------------GLAKLSRIPAFD 286
           ++YGYR K               VLLDPYAK+I  S             L +L + P FD
Sbjct: 304 VSYGYRFKGSFSQGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFD 363

Query: 287 WGGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLL 466
           WGGD+  +LPME+LVVYRLNV++F++ KSSKLPP  AGTF+GVTEK+ H K LGVNA+LL
Sbjct: 364 WGGDVHLNLPMEKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNAILL 423

Query: 467 EPVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFT 643
           EP+  FDE+KG Y+P HFFSP  L+GP     SAI SMKEMVK +HANGIEV LEVVFT
Sbjct: 424 EPILSFDEQKGPYFPRHFFSPTKLHGPSRGSISAINSMKEMVKKLHANGIEVLLEVVFT 482


>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
           gi|557546394|gb|ESR57372.1| hypothetical protein
           CICLE_v10023436mg [Citrus clementina]
          Length = 840

 Score =  276 bits (705), Expect = 5e-72
 Identities = 139/239 (58%), Positives = 172/239 (71%), Gaps = 25/239 (10%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FS  G+ NFA FSRNA  V+LCLY D+TA++PALE++LDPY+NR+GDIWHAS++S   +
Sbjct: 195 SFSIDGSLNFAIFSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMESTWNF 254

Query: 182 MNYGYRIKNK-------------VLLDPYAKVISKS------------GLAKLSRIPAFD 286
           ++YGYR K               VLLDPYAK+I  S             L +L + P FD
Sbjct: 255 VSYGYRFKGSFSQGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFD 314

Query: 287 WGGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLL 466
           WGGD+  +LPME+LVVYRLNV++F++ KSSKLPP  AGTF+GVTEK+ H K LGVNA+LL
Sbjct: 315 WGGDVHLNLPMEKLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNAILL 374

Query: 467 EPVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFT 643
           EP+  FDE+KG Y+P HFFSP  L+GP     SAI SMKEMVK +HANGIEV LEVVFT
Sbjct: 375 EPILSFDEQKGPYFPRHFFSPTKLHGPSRGSISAINSMKEMVKKLHANGIEVLLEVVFT 433


>gb|ESW21083.1| hypothetical protein PHAVU_005G040300g [Phaseolus vulgaris]
          Length = 853

 Score =  275 bits (704), Expect = 6e-72
 Identities = 135/234 (57%), Positives = 170/234 (72%), Gaps = 19/234 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           ++S  G+ NFA FSR+A+ V+LCLY +   EKPALE++LDP++NR+GD+WH S +S   Y
Sbjct: 225 SYSPDGSVNFAIFSRHAEGVVLCLYDEKGVEKPALEVDLDPFMNRSGDMWHVSFESVKSY 284

Query: 182 MNYGYRIKN----------KVLLDPYAKVISKS---------GLAKLSRIPAFDWGGDMP 304
           ++YGYR +            V+LDPYAK++  S          L  L + PAFDWGGD  
Sbjct: 285 VSYGYRCRGGVHNGDSSAEHVVLDPYAKIVGHSYPGGLGLVQNLGWLRKEPAFDWGGDFH 344

Query: 305 PSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPF 484
           P LPMEELVVYRLNV +FT+ KSS+LP   AG F G+ EK+QHFK LGVNAVLLEPVF F
Sbjct: 345 PDLPMEELVVYRLNVKRFTQHKSSQLPSGSAGIFTGLAEKVQHFKDLGVNAVLLEPVFTF 404

Query: 485 DERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           DE+KG Y+PSHFFS +++YGP GD  S + SMKEMVKTMHANGIEV +EVVF++
Sbjct: 405 DEKKGPYFPSHFFSLMHIYGPSGDPVSTMASMKEMVKTMHANGIEVLMEVVFSN 458


>gb|AAY89379.1| isoamylase isoform 2 [Nicotiana langsdorffii x Nicotiana sanderae]
          Length = 556

 Score =  275 bits (702), Expect = 1e-71
 Identities = 136/234 (58%), Positives = 168/234 (71%), Gaps = 19/234 (8%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           ++   G  NFA FSR+A SV+LCLY D + E P+LEI+LDPYVNR+GDIWHA+LD +  +
Sbjct: 91  SYQPDGTMNFALFSRSAKSVVLCLYDDISVENPSLEIDLDPYVNRSGDIWHAALDCSLPF 150

Query: 182 MNYGYRIK-------NKVLLDPYAKVISK------------SGLAKLSRIPAFDWGGDMP 304
             YGYR K         VLLDPY+KVI                L +L + P +DW GD+P
Sbjct: 151 KTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQGGSELRPKYLGELCKEPGYDWSGDVP 210

Query: 305 PSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPF 484
           PSLPME+L++YRLNV +FTKD SSKLP   AGTF+G+TEKL HFK LGVNAVLLEP+FPF
Sbjct: 211 PSLPMEKLIIYRLNVTEFTKDGSSKLPDDLAGTFSGITEKLSHFKHLGVNAVLLEPIFPF 270

Query: 485 DERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           +E+KG Y+PSHFFSP N++G   D  S I SM++MVK +HANG E+ LEVVFTH
Sbjct: 271 EEQKGPYFPSHFFSPGNMHGRSSDPLSVINSMEDMVKKLHANGKEIFLEVVFTH 324


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score =  274 bits (701), Expect = 1e-71
 Identities = 140/237 (59%), Positives = 176/237 (74%), Gaps = 22/237 (9%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           ++S  G+ NF+ FSR+A+SV+LCLY ++  EKPALE++LDPYVNRTGDIWH S +SA G+
Sbjct: 225 SYSPDGSVNFSIFSRHAESVVLCLYDENGVEKPALELDLDPYVNRTGDIWHVSFESAKGF 284

Query: 182 MNYGYRI------KNK-------VLLDPYAKVISKS-----GLAK----LSRIPAFDWGG 295
           ++YGYR       KNK       V+LDPYAK++  S     GL K    L + P FDWGG
Sbjct: 285 VSYGYRCRRGVLKKNKDDGFAEHVVLDPYAKIVGNSYPDGVGLVKNLGCLRKEPFFDWGG 344

Query: 296 DMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPV 475
           D  P L ME+LVVYRLNV +FT+ +SS+LP   AGTF G+ +K+QHFK LGVNAVLLEPV
Sbjct: 345 DRHPDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNAVLLEPV 404

Query: 476 FPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           F FDE+KG Y+P HFFS +++YGP G   SAI +MKEMVKTMHANGIEV +EVVF++
Sbjct: 405 FTFDEKKGPYFPCHFFSLMHIYGPSGGPVSAIAAMKEMVKTMHANGIEVLVEVVFSN 461


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  274 bits (700), Expect = 2e-71
 Identities = 141/239 (58%), Positives = 172/239 (71%), Gaps = 24/239 (10%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           +FSS G+ NFAFFSRNA  V+LCLY D T++KPALEI+LDPYVNRTGDIWHAS++S   +
Sbjct: 239 SFSSDGSPNFAFFSRNAGGVVLCLY-DGTSDKPALEIDLDPYVNRTGDIWHASMESVGSF 297

Query: 182 MNYGYRIKNKVL-------------LDPYAKVISKS-----------GLAKLSRIPAFDW 289
           ++YGYR K   L             LDPYAK+I  S            L +L + PAF+W
Sbjct: 298 VSYGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNW 357

Query: 290 GGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLE 469
             D+ P +PME+LVVYRLNV+ FTKD+SS++    AGTF+G+ EKL HFK LGVNAVLLE
Sbjct: 358 NDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLE 417

Query: 470 PVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           P+F FDE+KG Y+P HFFSP+N+YGP     S I S+KEMVK +HANGIEV LEVVFTH
Sbjct: 418 PIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTH 476


>ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Capsella rubella]
           gi|482571937|gb|EOA36124.1| hypothetical protein
           CARUB_v10012076mg [Capsella rubella]
          Length = 882

 Score =  273 bits (698), Expect = 3e-71
 Identities = 132/224 (58%), Positives = 165/224 (73%), Gaps = 17/224 (7%)
 Frame = +2

Query: 26  NFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGYMNYGYRIK 205
           NFAFFSRN+ SV+LCLY DST +KPALE++LDPYVNRTGD+WHAS+D+   ++ YGYR K
Sbjct: 246 NFAFFSRNSTSVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCK 305

Query: 206 NK-------------VLLDPYAKVISKSG----LAKLSRIPAFDWGGDMPPSLPMEELVV 334
                          V LDPYA VI KS     L  L + P+FDWG D+ P++P+E+L+V
Sbjct: 306 EMAQSEEDEDVEAQPVALDPYATVIDKSFSQKFLGSLLKTPSFDWGEDVSPNIPLEKLIV 365

Query: 335 YRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPFDERKGQYYPS 514
           YRLNV  FT+ KSSKLP    GTF+GV EK+ H K+LG+NAVLLEP+F F E+KG Y+P 
Sbjct: 366 YRLNVKGFTQHKSSKLPSNVTGTFSGVAEKVNHLKTLGINAVLLEPIFSFSEQKGPYFPV 425

Query: 515 HFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           HFFSP+++YGP   + SA+ SMKEMVK +H+ GIEV LEVVFTH
Sbjct: 426 HFFSPMDMYGPSNGFESAVKSMKEMVKKLHSQGIEVLLEVVFTH 469


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
           [Cucumis sativus]
          Length = 885

 Score =  272 bits (695), Expect = 7e-71
 Identities = 131/242 (54%), Positives = 178/242 (73%), Gaps = 27/242 (11%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           + S  G+ NF+ FS +A+S++LCLY DST+EKP LE++LDPY+NR+G+IWHAS + AS +
Sbjct: 239 SISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 298

Query: 182 MNYGYRIK-------------NKVLLDPYAKVISKS--------------GLAKLSRIPA 280
           ++YGY+ K             +++++DPYAK+++ S               L ++S++P 
Sbjct: 299 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 358

Query: 281 FDWGGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAV 460
           FDW G++ P+LPME+L VYRLNV +FT DKSS+LP   AGTF+G+T+KL HFK+LGVNAV
Sbjct: 359 FDWDGEVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 418

Query: 461 LLEPVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVF 640
           LLEP+F FDE++G Y+P HFFSP N YGP G   SAI SMKEMVK +HANG+EV LEVV+
Sbjct: 419 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 478

Query: 641 TH 646
           TH
Sbjct: 479 TH 480


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  272 bits (695), Expect = 7e-71
 Identities = 131/242 (54%), Positives = 178/242 (73%), Gaps = 27/242 (11%)
 Frame = +2

Query: 2   AFSSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGY 181
           + S  G+ NF+ FS +A+S++LCLY DST+EKP LE++LDPY+NR+G+IWHAS + AS +
Sbjct: 239 SISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKF 298

Query: 182 MNYGYRIK-------------NKVLLDPYAKVISKS--------------GLAKLSRIPA 280
           ++YGY+ K             +++++DPYAK+++ S               L ++S++P 
Sbjct: 299 VSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPT 358

Query: 281 FDWGGDMPPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAV 460
           FDW G++ P+LPME+L VYRLNV +FT DKSS+LP   AGTF+G+T+KL HFK+LGVNAV
Sbjct: 359 FDWDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAV 418

Query: 461 LLEPVFPFDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVF 640
           LLEP+F FDE++G Y+P HFFSP N YGP G   SAI SMKEMVK +HANG+EV LEVV+
Sbjct: 419 LLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVY 478

Query: 641 TH 646
           TH
Sbjct: 479 TH 480


>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 866

 Score =  269 bits (687), Expect = 6e-70
 Identities = 136/235 (57%), Positives = 168/235 (71%), Gaps = 22/235 (9%)
 Frame = +2

Query: 8   SSGGATNFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGYMN 187
           S  G+ NFA FSR A+SV+LCLY ++  EKPALE++LDPYVNRTGDIWH + +SA G+M+
Sbjct: 228 SPDGSVNFAIFSRRAESVVLCLYDENDMEKPALELDLDPYVNRTGDIWHVAFESAKGFMS 287

Query: 188 YGYRIKNKVL-------------LDPYAKVISKS---------GLAKLSRIPAFDWGGDM 301
           YGY  +  VL             LDPYAK++  S          L  L + P FDWGGD 
Sbjct: 288 YGYSCRGGVLKRNKDDGFAEHVVLDPYAKIVGNSYPDGVGFVKNLGWLGKEPDFDWGGDC 347

Query: 302 PPSLPMEELVVYRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFP 481
              L ME+LVVYRLNV +FT+ +SS+LP   AGTF G+ +K+QHFK LGVNA+LLEPVF 
Sbjct: 348 HLDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNAILLEPVFT 407

Query: 482 FDERKGQYYPSHFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           FDE+KG Y+PSHFFS +++YGP G   SAI SMKEMVKTMHANGIEV +EVVF++
Sbjct: 408 FDEKKGPYFPSHFFSLMHIYGPSGGPVSAIASMKEMVKTMHANGIEVLVEVVFSN 462


>ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata subsp. lyrata]
           gi|297335294|gb|EFH65711.1| ATISA2/BE2/DBE1/ISA2
           [Arabidopsis lyrata subsp. lyrata]
          Length = 872

 Score =  269 bits (687), Expect = 6e-70
 Identities = 130/224 (58%), Positives = 164/224 (73%), Gaps = 17/224 (7%)
 Frame = +2

Query: 26  NFAFFSRNADSVILCLYKDSTAEKPALEIELDPYVNRTGDIWHASLDSASGYMNYGYRIK 205
           NFAFFSRN+ +V+LCLY D+T  KPALE++LDPYVNRTGD+WHAS+D+   ++ YGYR K
Sbjct: 236 NFAFFSRNSTNVVLCLYDDTTTNKPALELDLDPYVNRTGDVWHASIDNTWDFVRYGYRCK 295

Query: 206 NK-------------VLLDPYAKVISKSG----LAKLSRIPAFDWGGDMPPSLPMEELVV 334
                          ++LDPYA VI KS     L  L + P+FDWG D+ P++P+E+L+V
Sbjct: 296 ETAHSEEDVDVEAEPIVLDPYATVIGKSVSQKFLGSLFKNPSFDWGEDVSPNIPLEKLIV 355

Query: 335 YRLNVLKFTKDKSSKLPPRYAGTFAGVTEKLQHFKSLGVNAVLLEPVFPFDERKGQYYPS 514
           YRLNV  FT+ KSSKLP   AGTF+GV EK+ H K+LGVN VLLEP+F F E+KG Y+P 
Sbjct: 356 YRLNVKGFTQHKSSKLPSNVAGTFSGVAEKVSHLKTLGVNGVLLEPIFSFSEQKGPYFPF 415

Query: 515 HFFSPVNLYGPFGDYRSAIVSMKEMVKTMHANGIEVHLEVVFTH 646
           HFFSP+++YGP     SA+ SMKEMVK +H+ GIEV LEVVFTH
Sbjct: 416 HFFSPMDIYGPSNSLESAVNSMKEMVKKLHSQGIEVLLEVVFTH 459