BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rheum21_contig00012531
         (1048 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  328   2e-87
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   328   2e-87
emb|CAN73910.1| hypothetical protein VITISV_031171 [Vitis vinifera]   328   3e-87
dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]              318   2e-84
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        318   3e-84
emb|CBI26520.3| unnamed protein product [Vitis vinifera]              317   5e-84
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          316   8e-84
gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana...   315   2e-83
ref|NP_001237270.1| 4-coumarate:CoA ligase isoenzyme 3 [Glycine ...   314   4e-83
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]          313   5e-83
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   313   5e-83
gb|AGO89321.1| Ca4CL4 [Salix arbutifolia]                             313   7e-83
gb|ADU15550.1| 4CL [Gossypium hirsutum]                               313   9e-83
gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]             311   2e-82
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            311   2e-82
ref|XP_003610843.1| 4-coumarate CoA ligase [Medicago truncatula]...   311   3e-82
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  311   4e-82
gb|ESW29061.1| hypothetical protein PHAVU_002G040100g [Phaseolus...   310   5e-82
gb|AGW47866.1| 4-coumarate:coenzyme A ligase 1 [Phaseolus vulgaris]   307   4e-81
sp|P31687.2|4CL2_SOYBN RecName: Full=4-coumarate--CoA ligase 2; ...   306   7e-81

>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  328 bits (842), Expect = 2e-87
 Identities = 158/244 (64%), Positives = 193/244 (79%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +H+F+S+LP IPISNHLPLH YCF N  +  DRPCLIVGST K +SFAETHL+ RK  AG
Sbjct: 29   DHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNKTYSFAETHLISRKVGAG 88

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
             ++LG+++GD +M+LL N            M+GAV TTANP+YTS+EI KQL+ SKAK+V
Sbjct: 89   FAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFYTSAEIFKQLNASKAKIV 148

Query: 676  ITHAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEG 855
            +T AQYVDKLR++    ++   +GF VIT+DDPPENC+HFSV+S+A++ +L      E  
Sbjct: 149  VTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPENCMHFSVVSEANESELP-----EVS 203

Query: 856  FDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFH 1035
             +S + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC+LPLFH
Sbjct: 204  INSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCVLPLFH 263

Query: 1036 IYSL 1047
            IYSL
Sbjct: 264  IYSL 267


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  328 bits (842), Expect = 2e-87
 Identities = 158/244 (64%), Positives = 193/244 (79%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +H+F+S+LP IPISNHLPLH YCF N  +  DRPCLIVGST K +SFAETHL+ RK  AG
Sbjct: 29   DHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNKTYSFAETHLISRKVGAG 88

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
             ++LG+++GD +M+LL N            M+GAV TTANP+YTS+EI KQL+ SKAK+V
Sbjct: 89   FAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFYTSAEIFKQLNASKAKIV 148

Query: 676  ITHAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEG 855
            +T AQYVDKLR++    ++   +GF VIT+DDPPENC+HFSV+S+A++ +L      E  
Sbjct: 149  VTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPENCMHFSVVSEANESELP-----EVS 203

Query: 856  FDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFH 1035
             +S + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC+LPLFH
Sbjct: 204  INSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCVLPLFH 263

Query: 1036 IYSL 1047
            IYSL
Sbjct: 264  IYSL 267


>emb|CAN73910.1| hypothetical protein VITISV_031171 [Vitis vinifera]
          Length = 531

 Score =  328 bits (840), Expect = 3e-87
 Identities = 158/244 (64%), Positives = 192/244 (78%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +H+F+S+LP IPISNHLPLH YCF N  +  DRPCLIVGST K +SFAETHL+ RK  AG
Sbjct: 7    DHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNKTYSFAETHLISRKVGAG 66

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
             ++LG+++GD +M+LL N            M+GAV TTANP+YTS+EI KQL+ SKAK+V
Sbjct: 67   FAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFYTSAEIFKQLNASKAKIV 126

Query: 676  ITHAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEG 855
            +T AQYVDKLR++    ++   +GF VIT+DDPPENC+HFSV S+A++ +L      E  
Sbjct: 127  VTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPENCMHFSVXSEANESELP-----EVS 181

Query: 856  FDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFH 1035
             +S + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC+LPLFH
Sbjct: 182  INSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCVLPLFH 241

Query: 1036 IYSL 1047
            IYSL
Sbjct: 242  IYSL 245


>dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]
          Length = 569

 Score =  318 bits (815), Expect = 2e-84
 Identities = 164/243 (67%), Positives = 189/243 (77%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            HIF+SRLP IPI N +PLH YCF NL E  DR CLIVGSTGK +SFAETHL+CRK A+GL
Sbjct: 27   HIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDRTCLIVGSTGKTYSFAETHLICRKVASGL 86

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            + LGV+KGD IM L  N            MIGAV TTANP+YT +EI KQ+S SKAKVVI
Sbjct: 87   AKLGVKKGDVIMTLFQNCPEFVFSFMGASMIGAVTTTANPFYTKAEIFKQMSASKAKVVI 146

Query: 679  THAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEGF 858
            T + YVDKL++       +G+D F V+T+DDPPENCLHFSVLS+AD++D+    TVE   
Sbjct: 147  TQSVYVDKLKDSGEEYPKIGED-FSVVTIDDPPENCLHFSVLSEADEEDMPA--TVEIAP 203

Query: 859  DSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFHI 1038
            D  +AVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL+L  DDVVLC+LPLFHI
Sbjct: 204  D--DAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHI 261

Query: 1039 YSL 1047
            +SL
Sbjct: 262  FSL 264


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  318 bits (814), Expect = 3e-84
 Identities = 157/251 (62%), Positives = 192/251 (76%), Gaps = 8/251 (3%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+F+S+LP IPI NHLPLH YCF NLPE  D+PCLIVGSTGK +SF+ETHL+ +KT AGL
Sbjct: 58   HVFRSKLPDIPIPNHLPLHTYCFQNLPEFSDKPCLIVGSTGKSYSFSETHLISQKTGAGL 117

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG++KGD IM+LL N            +IGAV TTANP+YTS+EI KQ+  + AK++I
Sbjct: 118  SNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKLII 177

Query: 679  THAQYVDKLREHHPAGLSVGD--------DGFVVITVDDPPENCLHFSVLSDADDKDLKV 834
            T +QYVDKLRE HP+     D        + F V+T+DDPPENCLHFSV S+A++K+L  
Sbjct: 178  TQSQYVDKLRE-HPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELP- 235

Query: 835  KETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVL 1014
                +   D+ + VALPFSSGTTGLPKGV+LTHKSL+TSVAQQVDGENPNL+L  DDVVL
Sbjct: 236  ----DVVIDAEDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVL 291

Query: 1015 CLLPLFHIYSL 1047
            C+LPLFHI+SL
Sbjct: 292  CVLPLFHIFSL 302


>emb|CBI26520.3| unnamed protein product [Vitis vinifera]
          Length = 512

 Score =  317 bits (812), Expect = 5e-84
 Identities = 156/244 (63%), Positives = 187/244 (76%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +H+F+S+LP IPISNHLPLH YCF N  +  DRPCLIVGST K +SFAETHL+ RK  AG
Sbjct: 7    DHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNKTYSFAETHLISRKVGAG 66

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
             ++LG+++GD +M+LL N            M+GAV TTANP+YTS+EI KQL+ SKAK+V
Sbjct: 67   FAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFYTSAEIFKQLNASKAKIV 126

Query: 676  ITHAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEG 855
            +T AQYV K+ E           GF VIT+DDPPENC+HFSV+S+A++ +L      E  
Sbjct: 127  VTQAQYVAKIGE-----------GFTVITIDDPPENCMHFSVVSEANESELP-----EVS 170

Query: 856  FDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFH 1035
             +S + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC+LPLFH
Sbjct: 171  INSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCVLPLFH 230

Query: 1036 IYSL 1047
            IYSL
Sbjct: 231  IYSL 234


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  316 bits (810), Expect = 8e-84
 Identities = 161/252 (63%), Positives = 195/252 (77%), Gaps = 8/252 (3%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +HIF+S+LP IPISNHLPLH YCF NLP + D+PCLI GS+GKI+SF+ETHL+ +KTAAG
Sbjct: 26   DHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCLISGSSGKIYSFSETHLIAQKTAAG 85

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
            LSNLG++KGDAIM+LLHN            MIGAV TTANP+YTS EI KQ   ++AK++
Sbjct: 86   LSNLGIQKGDAIMILLHNCAEFVFAFMGASMIGAVSTTANPFYTSKEIFKQFKAARAKLI 145

Query: 676  ITHAQYVDKLR--------EHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLK 831
            IT +QYVDKL+        E+ P    +G+D F VIT+DDPPENCL FS+LS+A++ ++ 
Sbjct: 146  ITQSQYVDKLKDTNNNDGDENFP---KIGED-FKVITIDDPPENCLDFSMLSEANENEIP 201

Query: 832  VKETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVV 1011
                 +      + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL+LT DDVV
Sbjct: 202  -----QVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTHDDVV 256

Query: 1012 LCLLPLFHIYSL 1047
            LC+LPLFHIYSL
Sbjct: 257  LCVLPLFHIYSL 268


>gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana var. lobata]
          Length = 567

 Score =  315 bits (806), Expect = 2e-83
 Identities = 157/243 (64%), Positives = 190/243 (78%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+FKS+LP IPISNH+PLHAYCF  L +  DRPCLIVG+  K +++AETHL+ RK AAGL
Sbjct: 30   HVFKSKLPDIPISNHIPLHAYCFEKLSDFADRPCLIVGAASKTYTYAETHLISRKIAAGL 89

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG+RKGD +M+LL N            MIGAV TTANP+YT++EI KQ ++SKAK++I
Sbjct: 90   SNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAVGTTANPFYTAAEIFKQFTVSKAKLII 149

Query: 679  THAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEGF 858
            T A YVDKLR H   G ++G+D F V+TVDDPPENCLHFSVLS+A++      E  E   
Sbjct: 150  TQAMYVDKLRHHD--GTNLGED-FKVVTVDDPPENCLHFSVLSEANE-----SEVPEVEI 201

Query: 859  DSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFHI 1038
               +AVA+PFSSGTTGLPKGVVLTHKSL TSVAQQVDGENPNL+LT +DV+LC+LPLFHI
Sbjct: 202  HPDDAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHI 261

Query: 1039 YSL 1047
            +SL
Sbjct: 262  FSL 264


>ref|NP_001237270.1| 4-coumarate:CoA ligase isoenzyme 3 [Glycine max]
            gi|4038971|gb|AAC97389.1| 4-coumarate:CoA ligase
            isoenzyme 3 [Glycine max] gi|4038973|gb|AAC97599.1|
            4-coumarate:CoA ligase isoenzyme 3 [Glycine max]
          Length = 570

 Score =  314 bits (804), Expect = 4e-83
 Identities = 156/243 (64%), Positives = 188/243 (77%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+FKS+LP IPISNHLPLHAYCF  L +  DRPCLIVG   K ++++ETHL+ RK AAGL
Sbjct: 32   HVFKSKLPDIPISNHLPLHAYCFQKLSQFSDRPCLIVGPAAKTYTYSETHLISRKIAAGL 91

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG+RKGD +M+LL N            MIGAV TTANP+YT++EI KQ ++SK K++I
Sbjct: 92   SNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAVATTANPFYTAAEIFKQFTVSKTKLII 151

Query: 679  THAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEGF 858
            T A YVDKLR +H  G  +G+D F V+TVDDPPENCLHFSVLS+A++ D       E   
Sbjct: 152  TQAMYVDKLR-NHDDGAKLGED-FKVVTVDDPPENCLHFSVLSEANESD-----APEVDI 204

Query: 859  DSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFHI 1038
               +AVA+PFSSGTTGLPKGVVLTHKSL TSVAQQVDGENPNL+LT +DV+LC+LPLFHI
Sbjct: 205  QPDDAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHI 264

Query: 1039 YSL 1047
            +SL
Sbjct: 265  FSL 267


>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
          Length = 574

 Score =  313 bits (803), Expect = 5e-83
 Identities = 159/250 (63%), Positives = 196/250 (78%), Gaps = 6/250 (2%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +HIF+S+LP IPISNHLPLH YCF NLP + D+PCLI GS+GKI+SF+ETHL+ +KTAAG
Sbjct: 26   DHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCLISGSSGKIYSFSETHLIAQKTAAG 85

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
            LS LG++KGDAIM+LLHN            MIGAV TTANP+YTS EI KQ   ++AK++
Sbjct: 86   LSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAVSTTANPFYTSKEIFKQFKAARAKLI 145

Query: 676  ITHAQYVDKLRE--HHPAGL----SVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVK 837
            IT +QYVDKL++  ++  G+     +G+D F VIT+DDPPENCL FSVLS+A++ ++   
Sbjct: 146  ITQSQYVDKLKDTNNNNDGVENFPKIGED-FKVITIDDPPENCLDFSVLSEANENEIP-- 202

Query: 838  ETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC 1017
               +      + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL+LT +DVVLC
Sbjct: 203  ---QVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTHEDVVLC 259

Query: 1018 LLPLFHIYSL 1047
            +LPLFHIYSL
Sbjct: 260  VLPLFHIYSL 269


>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 573

 Score =  313 bits (803), Expect = 5e-83
 Identities = 160/249 (64%), Positives = 194/249 (77%), Gaps = 1/249 (0%)
 Frame = +1

Query: 304  SDSPEHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRK 483
            S S  HIF+S+LP IPISNHLPLH YCF NL ++ D+PCLI GSTGK +SFAETHL+ +K
Sbjct: 28   SSSETHIFRSKLPDIPISNHLPLHTYCFENLFKLSDKPCLISGSTGKTYSFAETHLISQK 87

Query: 484  TAAGLSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSK 663
            TAAGLS+LG++KGD IM+LL N            MIGAV TTANP+YTS+EI KQL+ S+
Sbjct: 88   TAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGASMIGAVATTANPFYTSNEIFKQLNSSR 147

Query: 664  AKVVITHAQYVDKLRE-HHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKE 840
             K++IT + YVDKLR+  H     +G+D F VIT+D+PPENCLHF+VLS+A + +  + E
Sbjct: 148  PKLIITQSLYVDKLRDPDHKIQPKLGED-FTVITIDEPPENCLHFTVLSEASETE--IPE 204

Query: 841  TVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCL 1020
             +    D  + VALPFSSGTTGLPKGV+LTHKSLITSVAQQVDGENPNL+L  DDVVLC+
Sbjct: 205  VINANPD--DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCV 262

Query: 1021 LPLFHIYSL 1047
            LPLFHIYSL
Sbjct: 263  LPLFHIYSL 271


>gb|AGO89321.1| Ca4CL4 [Salix arbutifolia]
          Length = 559

 Score =  313 bits (802), Expect = 7e-83
 Identities = 158/250 (63%), Positives = 189/250 (75%), Gaps = 2/250 (0%)
 Frame = +1

Query: 304  SDSPEHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRK 483
            SD  +H+F+S+LP IPISNHLPLH YCF NL +  DRPCLI GSTGK +SFAETHL+ RK
Sbjct: 25   SDETDHVFRSKLPDIPISNHLPLHTYCFENLSDFSDRPCLISGSTGKTYSFAETHLVSRK 84

Query: 484  TAAGLSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSK 663
             AAGLSNLG++KGD IM LL N            MIGAV TTANP+YT SEI KQ S S+
Sbjct: 85   VAAGLSNLGIKKGDVIMTLLQNCPEFIFSFVGASMIGAVTTTANPFYTPSEIFKQFSASR 144

Query: 664  AKVVITHAQYVDKLREHH--PAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVK 837
            AK++IT +QYV KLR+        + G+D FVVIT+DDPPENCLHF+VL++A++ ++   
Sbjct: 145  AKLIITQSQYVTKLRDSDCCENSKNPGED-FVVITIDDPPENCLHFTVLAEANESEMPT- 202

Query: 838  ETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC 1017
                      + VALPFSSGTTGLPKGV+LTHKSL+TSVAQQVDGENPNL+L  DDVVLC
Sbjct: 203  ----VSIHPDDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKQDDVVLC 258

Query: 1018 LLPLFHIYSL 1047
            +LPLFHI+SL
Sbjct: 259  VLPLFHIFSL 268


>gb|ADU15550.1| 4CL [Gossypium hirsutum]
          Length = 574

 Score =  313 bits (801), Expect = 9e-83
 Identities = 159/250 (63%), Positives = 196/250 (78%), Gaps = 6/250 (2%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +HIF+S+LP IPISNHLPLH YCF NLP + D+PCLI GS+GKI+SF+ETHL+ +KTAAG
Sbjct: 26   DHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCLISGSSGKIYSFSETHLIAQKTAAG 85

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
            LS LG++KGDAIM+LLHN            MIGAV TTANP+YTS EI KQ   ++AK++
Sbjct: 86   LSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAVSTTANPFYTSKEIFKQSKAARAKLI 145

Query: 676  ITHAQYVDKLRE--HHPAGL----SVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVK 837
            IT +QYVDKL++  ++  G+     +G+D F VIT+DDPPENCL FSVLS+A++ ++   
Sbjct: 146  ITQSQYVDKLKDTNNNNDGVENFPKIGED-FKVITIDDPPENCLDFSVLSEANENEIP-- 202

Query: 838  ETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLC 1017
               +      + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL+LT +DVVLC
Sbjct: 203  ---QVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTHEDVVLC 259

Query: 1018 LLPLFHIYSL 1047
            +LPLFHIYSL
Sbjct: 260  VLPLFHIYSL 269


>gb|EOY10077.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
          Length = 575

 Score =  311 bits (798), Expect = 2e-82
 Identities = 156/246 (63%), Positives = 193/246 (78%), Gaps = 2/246 (0%)
 Frame = +1

Query: 316  EHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAG 495
            +HIF+S+LP IPISNHLPLH YCF NL    D+PCLI GS+GK +SF+ETHL+ +KTAAG
Sbjct: 33   DHIFRSKLPDIPISNHLPLHTYCFENLSSFPDKPCLISGSSGKTYSFSETHLIAQKTAAG 92

Query: 496  LSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVV 675
            LSNLG+RKGD IM+LL N            MIGAV TTANP+YTS+EI KQ  +++AK++
Sbjct: 93   LSNLGIRKGDVIMILLQNCAEFVFSFMGASMIGAVSTTANPFYTSTEIFKQYKVAQAKLI 152

Query: 676  ITHAQYVDKLRE--HHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVE 849
            +T +QYVDKL++  ++     +G+D F VIT+DDPPENCLHF+VLS+A++ ++      +
Sbjct: 153  VTQSQYVDKLKDTSNNENFPKMGED-FKVITIDDPPENCLHFTVLSEANENEIP-----Q 206

Query: 850  EGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPL 1029
               D  + VALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL+L  DDVVLC+LPL
Sbjct: 207  VSIDPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLKHDDVVLCVLPL 266

Query: 1030 FHIYSL 1047
            FHIYSL
Sbjct: 267  FHIYSL 272


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  311 bits (798), Expect = 2e-82
 Identities = 154/250 (61%), Positives = 191/250 (76%), Gaps = 8/250 (3%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+F+S+LP I I NHLPLH YCF NLPE  DRPCLIVGSTGK +SF+ETHL+ +KT AGL
Sbjct: 58   HVFRSKLPDIAIPNHLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGAGL 117

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG++KGD IM+LL N            +IGAV TTANP+YT++EI KQ+  + AK++I
Sbjct: 118  SNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKLII 177

Query: 679  THAQYVDKLREHHPAGLSVGD--------DGFVVITVDDPPENCLHFSVLSDADDKDLKV 834
            T +QYV+KLRE HP+     D        + F V+T+DDPPENCLHFSVLS+A++K+L  
Sbjct: 178  TQSQYVNKLRE-HPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVLSEANEKELP- 235

Query: 835  KETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVL 1014
                +   D+ + VALPFSSGTTGLPKGV+LTHK+L+TSVAQQVDGENPNL+L  DDVVL
Sbjct: 236  ----DVVIDAEDPVALPFSSGTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVL 291

Query: 1015 CLLPLFHIYS 1044
            C+LPLFHI+S
Sbjct: 292  CVLPLFHIFS 301


>ref|XP_003610843.1| 4-coumarate CoA ligase [Medicago truncatula]
            gi|355512178|gb|AES93801.1| 4-coumarate CoA ligase
            [Medicago truncatula]
          Length = 587

 Score =  311 bits (797), Expect = 3e-82
 Identities = 155/248 (62%), Positives = 188/248 (75%)
 Frame = +1

Query: 304  SDSPEHIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRK 483
            S+   HIFKS+LP IPISNHLPLH YCF NL E  DRPCLIV +TGK +++AET+L C+K
Sbjct: 49   SNQTTHIFKSKLPDIPISNHLPLHTYCFENLSEFADRPCLIVAATGKTYTYAETYLQCQK 108

Query: 484  TAAGLSNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSK 663
             AAGLS LG++KGD IM+LL N            MIGAV TTANP+YTS+EI KQ+  S+
Sbjct: 109  IAAGLSKLGIQKGDVIMILLQNSAEFVLSFIAASMIGAVATTANPFYTSAEIFKQIKASE 168

Query: 664  AKVVITHAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKET 843
             K++IT A YVDKLR+    G    +  F VIT D+PP NCLHFSV+S+++++D+ V E 
Sbjct: 169  TKLIITQAMYVDKLRQKDQVG---AEFDFKVITTDEPPLNCLHFSVISESNEEDIPVVE- 224

Query: 844  VEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLL 1023
                 D  +AVALPFSSGTTGLPKGV+LTHKSL TSVAQQVDGENPNLHLT +DV+LC+L
Sbjct: 225  ----IDPEDAVALPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLHLTTEDVLLCVL 280

Query: 1024 PLFHIYSL 1047
            PLFHI+SL
Sbjct: 281  PLFHIFSL 288


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  311 bits (796), Expect = 4e-82
 Identities = 158/251 (62%), Positives = 189/251 (75%), Gaps = 8/251 (3%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+FKS+LP IPI NHLPLH YCF  L E  DRPCLIV STGK +SF++T+L+ +KT AGL
Sbjct: 58   HVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIVSSTGKSYSFSDTYLISQKTGAGL 117

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG++KGD IM+LL N            MIGA+ TTANP+YT++EI KQ   S AK++I
Sbjct: 118  SNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNAKLII 177

Query: 679  THAQYVDKLREHHPAG--------LSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKV 834
            TH+Q+VDKLRE    G        L +G+  F V+TVDDPPENCLHFSV+S+A++K+L  
Sbjct: 178  THSQFVDKLREAPADGAAPDQNNYLKLGEH-FKVVTVDDPPENCLHFSVISEANEKELP- 235

Query: 835  KETVEEGFDSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVL 1014
                E   D  + VALPFSSGTTGLPKGV+LTHKSLITSVAQQVDGENPNL+L  DDVVL
Sbjct: 236  ----EVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVL 291

Query: 1015 CLLPLFHIYSL 1047
            C+LPLFHIYSL
Sbjct: 292  CVLPLFHIYSL 302


>gb|ESW29061.1| hypothetical protein PHAVU_002G040100g [Phaseolus vulgaris]
          Length = 566

 Score =  310 bits (795), Expect = 5e-82
 Identities = 155/243 (63%), Positives = 188/243 (77%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+FKS+LP IPISNHL LH YCF NL +  DRPCLIVGS  K +++A+T+L+ RK AAG 
Sbjct: 24   HVFKSKLPDIPISNHLSLHDYCFQNLSQFADRPCLIVGSASKTYTYAQTYLISRKIAAGF 83

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG+RKGD +M+LL N            M+GAV TTANP+YT++EI KQ + SKAK++I
Sbjct: 84   SNLGIRKGDVVMILLQNSAEFVLSFLAASMVGAVATTANPFYTAAEIFKQFTASKAKLII 143

Query: 679  THAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEGF 858
            T A YV+KLR  H AG S+G+D F V+TVDDPPENCLHFSVLS+A++ D       E   
Sbjct: 144  TQAMYVEKLRS-HDAGWSLGED-FKVVTVDDPPENCLHFSVLSEANESD-----APEVEI 196

Query: 859  DSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFHI 1038
               +AVALPFSSGTTGLPKGV+LTHKSL TSVAQQVDGENPNL+LT +DV+LC+LPLFHI
Sbjct: 197  HPDDAVALPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHI 256

Query: 1039 YSL 1047
            +SL
Sbjct: 257  FSL 259


>gb|AGW47866.1| 4-coumarate:coenzyme A ligase 1 [Phaseolus vulgaris]
          Length = 570

 Score =  307 bits (787), Expect = 4e-81
 Identities = 154/243 (63%), Positives = 187/243 (76%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+FKS+LP IPISNHL LH YCF NL +  D PCLIVGS  K +++A+T+L+ RK AAG 
Sbjct: 27   HVFKSKLPDIPISNHLSLHDYCFQNLSQFADCPCLIVGSASKTYTYAQTYLISRKIAAGF 86

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG+RKGD +M+LL N            M+GAV TTANP+YT++EI KQ + SKAK++I
Sbjct: 87   SNLGIRKGDVVMILLQNSAEFVLSFLAASMVGAVATTANPFYTAAEIFKQFTASKAKLII 146

Query: 679  THAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEGF 858
            T A YV+KLR  H AG S+G+D F V+TVDDPPENCLHFSVLS+A++ D       E   
Sbjct: 147  TQAMYVEKLRS-HDAGWSLGED-FKVVTVDDPPENCLHFSVLSEANESD-----APEVEI 199

Query: 859  DSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFHI 1038
               +AVALPFSSGTTGLPKGV+LTHKSL TSVAQQVDGENPNL+LT +DV+LC+LPLFHI
Sbjct: 200  HPDDAVALPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHI 259

Query: 1039 YSL 1047
            +SL
Sbjct: 260  FSL 262


>sp|P31687.2|4CL2_SOYBN RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2; AltName: Full=Clone
            4CL16 gi|13559169|emb|CAC36095.1| 4-coumarate:Coenzyme A
            ligase isoenzyme 4 [Glycine max]
          Length = 562

 Score =  306 bits (785), Expect = 7e-81
 Identities = 153/243 (62%), Positives = 185/243 (76%)
 Frame = +1

Query: 319  HIFKSRLPPIPISNHLPLHAYCFHNLPEIGDRPCLIVGSTGKIHSFAETHLLCRKTAAGL 498
            H+FKS+LP IPISNHLPLH+YCF NL +   RPCLIVG   K  ++A+THL+  K AAGL
Sbjct: 25   HVFKSKLPDIPISNHLPLHSYCFQNLSQFAHRPCLIVGPASKTFTYADTHLISSKIAAGL 84

Query: 499  SNLGVRKGDAIMLLLHNXXXXXXXXXXXXMIGAVVTTANPYYTSSEIAKQLSLSKAKVVI 678
            SNLG+ KGD +M+LL N            MIGAV TTANP+YT+ EI KQ ++SKAK++I
Sbjct: 85   SNLGILKGDVVMILLQNSADFVFSFLAISMIGAVATTANPFYTAPEIFKQFTVSKAKLII 144

Query: 679  THAQYVDKLREHHPAGLSVGDDGFVVITVDDPPENCLHFSVLSDADDKDLKVKETVEEGF 858
            T A YVDKLR H   G  +G+D F V+TVDDPPENCLHFSVLS+A++ D+      E   
Sbjct: 145  TQAMYVDKLRNHD--GAKLGED-FKVVTVDDPPENCLHFSVLSEANESDVP-----EVEI 196

Query: 859  DSTEAVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCLLPLFHI 1038
               +AVA+PFSSGTTGLPKGV+LTHKSL TSVAQQVDGENPNL+LT +DV+LC+LPLFHI
Sbjct: 197  HPDDAVAMPFSSGTTGLPKGVILTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHI 256

Query: 1039 YSL 1047
            +SL
Sbjct: 257  FSL 259