BLASTX nr result
ID: Rheum21_contig00000960
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rheum21_contig00000960
(1529 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003543239.1| PREDICTED: proton pump-interactor 1-like iso... 278 3e-72
ref|XP_004487714.1| PREDICTED: proton pump-interactor 1-like [Ci... 277 1e-71
ref|XP_006582166.1| PREDICTED: proton pump-interactor 1-like iso... 273 1e-70
ref|XP_003527243.1| PREDICTED: proton pump-interactor 1-like iso... 273 1e-70
gb|ESW05176.1| hypothetical protein PHAVU_011G158400g [Phaseolus... 273 2e-70
emb|CAN81802.1| hypothetical protein VITISV_007014 [Vitis vinifera] 273 2e-70
ref|XP_002285016.2| PREDICTED: uncharacterized protein LOC100267... 272 2e-70
gb|ESW21460.1| hypothetical protein PHAVU_005G072500g [Phaseolus... 271 7e-70
emb|CBI20294.3| unnamed protein product [Vitis vinifera] 266 1e-68
gb|EXB42553.1| hypothetical protein L484_011324 [Morus notabilis] 263 2e-67
ref|XP_004141360.1| PREDICTED: proton pump-interactor 1-like [Cu... 257 1e-65
gb|EOY13017.1| Proton pump interactor 1 isoform 2 [Theobroma cacao] 256 2e-65
gb|EOY13016.1| Proton pump interactor 1 isoform 1 [Theobroma cacao] 256 2e-65
ref|XP_006464761.1| PREDICTED: proton pump-interactor 1-like iso... 256 2e-65
ref|XP_006451833.1| hypothetical protein CICLE_v10008004mg [Citr... 256 2e-65
ref|XP_003596760.1| hypothetical protein MTR_2g085330 [Medicago ... 252 3e-64
ref|XP_003596759.1| hypothetical protein MTR_2g085330 [Medicago ... 252 3e-64
ref|XP_002316898.2| hypothetical protein POPTR_0011s12040g [Popu... 250 1e-63
gb|ACJ85701.1| unknown [Medicago truncatula] 249 2e-63
ref|XP_002329130.1| predicted protein [Populus trichocarpa] 247 1e-62
>ref|XP_003543239.1| PREDICTED: proton pump-interactor 1-like isoform X1 [Glycine max]
gi|571501181|ref|XP_006594761.1| PREDICTED: proton
pump-interactor 1-like isoform X2 [Glycine max]
Length = 616
Score = 278 bits (712), Expect = 3e-72
Identities = 160/288 (55%), Positives = 202/288 (70%), Gaps = 2/288 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQ++REIKQLEGTR KVIANAAMRAK+QES+GQKE +QD+VKLIG
Sbjct: 190 LQYRIQHESIPLTEEKQILREIKQLEGTREKVIANAAMRAKLQESMGQKETIQDQVKLIG 249
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+++D LKAID DI SLQ +LT+V +KRDKA E++ +LRKQR+
Sbjct: 250 GDLDGVKKEREAIRSKIKQIDDALKAIDKDIQSLQEELTAVSQKRDKAFESMQQLRKQRE 309
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GN F+Q+RT+LN +ELAA+K++ AL E+S EVEKFMA WN K+FR DYEKRI AS
Sbjct: 310 EGNTYFYQSRTVLNKARELAAKKDINALDEVSQTEVEKFMALWNKDKAFRNDYEKRILAS 369
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGRMRNPDEKP +L+ K AE + A
Sbjct: 370 LDMRQLSRDGRMRNPDEKP--ILEEPKPAEAEALPKTFTKQPKEEPKPSPQETL----PA 423
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKET-VKHDVVDEAKL 663
+KESK K R D K+ + ++ E D Y + P+KE K +D AKL
Sbjct: 424 QKESKNKGR-DLKSKPESKDVAETDEYEFKNPQKEAPAKECEIDPAKL 470
>ref|XP_004487714.1| PREDICTED: proton pump-interactor 1-like [Cicer arietinum]
Length = 615
Score = 277 bits (708), Expect = 1e-71
Identities = 159/288 (55%), Positives = 204/288 (70%), Gaps = 2/288 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQL+REIKQLEGTR KVIANAAMR K+Q+++GQK+A+QD+VKLIG
Sbjct: 191 LQYRIQHESIPLTEEKQLLREIKQLEGTREKVIANAAMRTKLQDTIGQKDAIQDQVKLIG 250
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+++D LK ID DI SLQ +LT++ +KR+K+ E+I +LRKQRD
Sbjct: 251 GDLDGVKKERQAIRSKIKQIDDVLKTIDTDIQSLQDELTAITQKREKSFESIQQLRKQRD 310
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GN+ F+Q+RTLL +ELAA+K++ AL ELS EVEKFM+ WNS K+FR DYEKRI +S
Sbjct: 311 EGNSYFYQSRTLLTKARELAAKKDINALDELSQTEVEKFMSLWNSGKTFRDDYEKRILSS 370
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGRMRNPDEKP LL+ K AE + + ET+A A
Sbjct: 371 LDMRQLSRDGRMRNPDEKP--LLEEPKPAEAE-ALPKSVLKQAKEEPKPSPLETLA---A 424
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKET-VKHDVVDEAKL 663
+KESK K + ++ E D Y E P+KET K +D AKL
Sbjct: 425 QKESKNKAKDLKTNKSDNKDLAEVDDYEFENPQKETAAKEPAIDPAKL 472
>ref|XP_006582166.1| PREDICTED: proton pump-interactor 1-like isoform X2 [Glycine max]
gi|571462004|ref|XP_006582167.1| PREDICTED: proton
pump-interactor 1-like isoform X3 [Glycine max]
gi|571462006|ref|XP_006582168.1| PREDICTED: proton
pump-interactor 1-like isoform X4 [Glycine max]
Length = 621
Score = 273 bits (699), Expect = 1e-70
Identities = 158/287 (55%), Positives = 202/287 (70%), Gaps = 1/287 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L+YR+Q E I EKQ++REIKQLEGTR KVIANAAMRAK+Q+S+GQKEA+QD+VKLIG
Sbjct: 192 LHYRIQHESIPFAEEKQILREIKQLEGTREKVIANAAMRAKVQDSMGQKEAIQDQVKLIG 251
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+LED +KA+D DI SLQ +L +V EKR+KA E+I + RKQRD
Sbjct: 252 GDLDGVKKERQAIRSKIKQLEDTVKALDKDIQSLQDELGAVTEKREKAYESIQQQRKQRD 311
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
GN+ F+Q+RT+LN +ELAA+K++ A++EL+ EVEK M+ WNS KSFR DYEKR+ S
Sbjct: 312 QGNSYFYQSRTILNKARELAAKKDINAIEELAQTEVEKAMSLWNSDKSFRDDYEKRLLPS 371
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQL DGRMRNPDEKP LL+ K AE T ETV +
Sbjct: 372 LDMRQLCRDGRMRNPDEKP--LLEEPKPAETDTLPKSSVKQPKEEEPKSSPPETVPEQKI 429
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+K+K + D K+ L + E+ D Y ELPK K +D KL
Sbjct: 430 QKETKKKGK-DLKSNLN-KVLEDTDEYEFELPKVN--KEPPIDPEKL 472
>ref|XP_003527243.1| PREDICTED: proton pump-interactor 1-like isoform X1 [Glycine max]
Length = 665
Score = 273 bits (699), Expect = 1e-70
Identities = 158/287 (55%), Positives = 202/287 (70%), Gaps = 1/287 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L+YR+Q E I EKQ++REIKQLEGTR KVIANAAMRAK+Q+S+GQKEA+QD+VKLIG
Sbjct: 236 LHYRIQHESIPFAEEKQILREIKQLEGTREKVIANAAMRAKVQDSMGQKEAIQDQVKLIG 295
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+LED +KA+D DI SLQ +L +V EKR+KA E+I + RKQRD
Sbjct: 296 GDLDGVKKERQAIRSKIKQLEDTVKALDKDIQSLQDELGAVTEKREKAYESIQQQRKQRD 355
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
GN+ F+Q+RT+LN +ELAA+K++ A++EL+ EVEK M+ WNS KSFR DYEKR+ S
Sbjct: 356 QGNSYFYQSRTILNKARELAAKKDINAIEELAQTEVEKAMSLWNSDKSFRDDYEKRLLPS 415
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQL DGRMRNPDEKP LL+ K AE T ETV +
Sbjct: 416 LDMRQLCRDGRMRNPDEKP--LLEEPKPAETDTLPKSSVKQPKEEEPKSSPPETVPEQKI 473
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+K+K + D K+ L + E+ D Y ELPK K +D KL
Sbjct: 474 QKETKKKGK-DLKSNLN-KVLEDTDEYEFELPKVN--KEPPIDPEKL 516
>gb|ESW05176.1| hypothetical protein PHAVU_011G158400g [Phaseolus vulgaris]
Length = 632
Score = 273 bits (697), Expect = 2e-70
Identities = 156/287 (54%), Positives = 203/287 (70%), Gaps = 1/287 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L Y +Q E I L EKQ++REIKQLEGTR KV+ANA+MRAK+Q+SLGQKE +QD+VKLIG
Sbjct: 201 LQYHIQHESIPLAEEKQIIREIKQLEGTREKVLANASMRAKVQDSLGQKEDIQDQVKLIG 260
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDG KKER V++SKIK+L+D +KA+D DI SLQ +L +V EKRDKA E I +LRKQRD
Sbjct: 261 GDLDGAKKERQVIRSKIKQLDDGVKALDKDIQSLQEELGAVTEKRDKAYENIQQLRKQRD 320
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
GN+ F+Q+RT+LN +ELAA+K++ AL+E EVEK M+ WNS KSFR DYEKR+ S
Sbjct: 321 QGNSYFYQSRTILNKARELAAKKDINALEEFVQIEVEKAMSLWNSDKSFRDDYEKRLLPS 380
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGRMRNPDEKP LL+ K E T A ET +
Sbjct: 381 LDMRQLSRDGRMRNPDEKP--LLEVPKPIETGTLA-KSSVKQPKGESKPAPQETEPEQTF 437
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+K+K + D K+ ++ + E+ D Y E+PK+ TVK +++ KL
Sbjct: 438 QKETKKKGK-DMKSNVENKVLEDADEYEFEMPKETTVKEPPINQEKL 483
>emb|CAN81802.1| hypothetical protein VITISV_007014 [Vitis vinifera]
Length = 536
Score = 273 bits (697), Expect = 2e-70
Identities = 154/289 (53%), Positives = 199/289 (68%), Gaps = 1/289 (0%)
Frame = -1
Query: 1526 QRLNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKL 1347
Q L+Y MQ E I L EKQ++REIKQLE TR KVIA AAMRAKIQ+SLGQKEA+QD+VKL
Sbjct: 191 QSLHYHMQHESIPLAEEKQILREIKQLEATREKVIAGAAMRAKIQDSLGQKEAIQDQVKL 250
Query: 1346 IGTNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQ 1170
IG +LDGV+KE+ VV +KIK+L D+LK ID +I SL +L +V EKRDKA E + LRKQ
Sbjct: 251 IGVDLDGVRKEKQVVMAKIKQLRDDLKGIDSEIDSLMEELKAVSEKRDKAYEGLQALRKQ 310
Query: 1169 RDDGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRIS 990
RD+GNA F+QNR LLNN +ELA +K+++AL+ELS EVEKFM+ W+S+K+FR+DYEKRI
Sbjct: 311 RDEGNAHFYQNRALLNNARELAGKKDIKALEELSHSEVEKFMSLWSSNKAFREDYEKRIL 370
Query: 989 ASLDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVE 810
SLD RQLS DGRMRNPDEKPL +L+ +E +T A +T+ +
Sbjct: 371 PSLDSRQLSRDGRMRNPDEKPLVVLEAPTPSEXETVAKAPVKRSKEDSKPPPQHDTLPSQ 430
Query: 809 NAEKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+ + T+ + E+E +E +K + VD AKL
Sbjct: 431 KVQKEANDRATDSGITLERGDKEEKEIIPGLEKHQKGPSLDNEVDAAKL 479
>ref|XP_002285016.2| PREDICTED: uncharacterized protein LOC100267528 [Vitis vinifera]
Length = 627
Score = 272 bits (696), Expect = 2e-70
Identities = 154/289 (53%), Positives = 199/289 (68%), Gaps = 1/289 (0%)
Frame = -1
Query: 1526 QRLNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKL 1347
Q L+Y MQ E I L EKQ++REIKQLE TR KVIA AAMRAKIQ+SLGQKEA+QD+VKL
Sbjct: 191 QSLHYHMQHESIPLAEEKQILREIKQLEATREKVIAGAAMRAKIQDSLGQKEAIQDQVKL 250
Query: 1346 IGTNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQ 1170
IG +LDGV+KE+ VV +KIK+L D+LK ID +I SL +L +V EKRDKA E + LRKQ
Sbjct: 251 IGVDLDGVRKEKQVVMAKIKQLRDDLKGIDSEIDSLMEELKAVSEKRDKAYEGLQALRKQ 310
Query: 1169 RDDGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRIS 990
RD+GNA F+QNR LLNN +ELA +K+++AL+ELS EVEKFM+ W+S+K+FR+DYEKRI
Sbjct: 311 RDEGNAHFYQNRALLNNARELAGKKDIKALEELSHSEVEKFMSLWSSNKAFREDYEKRIL 370
Query: 989 ASLDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVE 810
SLD RQLS DGRMRNPDEKPL +L+ +E +T A +T+ +
Sbjct: 371 PSLDSRQLSRDGRMRNPDEKPLVVLEAPTPSETETVAKAPVKRSKEDSKPPPQHDTLPSQ 430
Query: 809 NAEKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+ + T+ + E+E +E +K + VD AKL
Sbjct: 431 KVQKEANDRATDSGITLERGDKEEKEIIPGLEKHQKGPSLDNEVDAAKL 479
>gb|ESW21460.1| hypothetical protein PHAVU_005G072500g [Phaseolus vulgaris]
Length = 616
Score = 271 bits (692), Expect = 7e-70
Identities = 156/288 (54%), Positives = 203/288 (70%), Gaps = 2/288 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQL+REIKQLEGTR KVIANAAMR K+QES+GQKEA+QD+VKLIG
Sbjct: 189 LQYRIQHESIPLTEEKQLLREIKQLEGTREKVIANAAMRTKLQESMGQKEAIQDQVKLIG 248
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+++D LKAID DI SLQ +LT+V +KRDK+ E++ +L++QR+
Sbjct: 249 GDLDGVKKEREAIRSKIKQIDDALKAIDKDIQSLQEELTAVSQKRDKSFESMQQLKRQRE 308
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GN+ F+++RT+LN ++LAA+K++ AL E+S EVE FMA WNS K+FR DYEKRI AS
Sbjct: 309 EGNSYFYRSRTVLNKARDLAAKKDINALDEVSQTEVETFMALWNSDKAFRNDYEKRILAS 368
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGRMRNPDEKP +L+ K AE + A
Sbjct: 369 LDMRQLSRDGRMRNPDEKP--ILEEPKPAEAEAIPKTVPKQPKEEPKPSPQETL----PA 422
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHD-VVDEAKL 663
+KESK K R D K+ +++ D Y E PKK T ++ +D AKL
Sbjct: 423 QKESKNKGR-DLKSKPDIKDSAVTDEYEFENPKKATPTNEPEIDPAKL 469
>emb|CBI20294.3| unnamed protein product [Vitis vinifera]
Length = 478
Score = 266 bits (681), Expect = 1e-68
Identities = 141/217 (64%), Positives = 174/217 (80%), Gaps = 1/217 (0%)
Frame = -1
Query: 1526 QRLNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKL 1347
Q L+Y MQ E I L EKQ++REIKQLE TR KVIA AAMRAKIQ+SLGQKEA+QD+VKL
Sbjct: 221 QSLHYHMQHESIPLAEEKQILREIKQLEATREKVIAGAAMRAKIQDSLGQKEAIQDQVKL 280
Query: 1346 IGTNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQ 1170
IG +LDGV+KE+ VV +KIK+L D+LK ID +I SL +L +V EKRDKA E + LRKQ
Sbjct: 281 IGVDLDGVRKEKQVVMAKIKQLRDDLKGIDSEIDSLMEELKAVSEKRDKAYEGLQALRKQ 340
Query: 1169 RDDGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRIS 990
RD+GNA F+QNR LLNN +ELA +K+++AL+ELS EVEKFM+ W+S+K+FR+DYEKRI
Sbjct: 341 RDEGNAHFYQNRALLNNARELAGKKDIKALEELSHSEVEKFMSLWSSNKAFREDYEKRIL 400
Query: 989 ASLDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSA 879
SLD RQLS DGRMRNPDEKPL +L+ +E +T A
Sbjct: 401 PSLDSRQLSRDGRMRNPDEKPLVVLEAPTPSETETVA 437
>gb|EXB42553.1| hypothetical protein L484_011324 [Morus notabilis]
Length = 686
Score = 263 bits (671), Expect = 2e-67
Identities = 151/290 (52%), Positives = 197/290 (67%), Gaps = 4/290 (1%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQ++REIKQLE TR KV+ANAAMRAKIQES+ QKE +QD+VKLIG
Sbjct: 245 LQYRIQHESIPLSEEKQILREIKQLESTREKVVANAAMRAKIQESMVQKEVLQDQVKLIG 304
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGV+KE+ V++KIK+LED LK ID DI SLQ +LT++ EKRDKA E I +LRK+RD
Sbjct: 305 GDLDGVRKEQQTVRAKIKQLEDALKVIDKDISSLQDELTAISEKRDKAREIIQQLRKERD 364
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
DGN+ F+Q+R++LN ++LAA+K+++AL++LS E EKFM+ W+S+KSFR DYEKRI S
Sbjct: 365 DGNSYFYQSRSVLNRARDLAAKKDIQALEDLSNLEFEKFMSLWSSTKSFRDDYEKRILPS 424
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD RQLS DGRMRNPDEKPL + + E +T A + + +
Sbjct: 425 LDSRQLSRDGRMRNPDEKPLVAPEDSAPVESETVA-KALPKRAKEDPKPPQVDALPAQKV 483
Query: 803 EKESKRKQRGDPKTILKPRNAEE---EDYYVVELPKKETVKHDVVDEAKL 663
+KE K K + AEE + +E PKK K + +D AKL
Sbjct: 484 QKEVKNKSTNSKPAVDHTDVAEEVPGPEKLPIETPKK---KDNEIDAAKL 530
>ref|XP_004141360.1| PREDICTED: proton pump-interactor 1-like [Cucumis sativus]
gi|449498721|ref|XP_004160615.1| PREDICTED: proton
pump-interactor 1-like [Cucumis sativus]
Length = 639
Score = 257 bits (656), Expect = 1e-65
Identities = 148/290 (51%), Positives = 200/290 (68%), Gaps = 2/290 (0%)
Frame = -1
Query: 1526 QRLNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKL 1347
Q L Y +Q E I L EKQ++REIKQLEGTR KVIANAAMRAK+Q+S+ QKEA+QD+VK+
Sbjct: 187 QSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQKEALQDQVKI 246
Query: 1346 IGTNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQ 1170
IG +LDGV+KE+ V++KIK+L+D LKAID +I +LQ +LTSV EKR +A E+I +LRK
Sbjct: 247 IGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAHESIQQLRKN 306
Query: 1169 RDDGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRIS 990
RD+GNA F+Q+R+LLN ++LAA+K+++AL+EL+ EVEKFM+ WN K+FR +YEKRI
Sbjct: 307 RDEGNAHFYQSRSLLNKARDLAAQKDVKALEELASNEVEKFMSLWNGDKAFRDEYEKRIL 366
Query: 989 ASLDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTS-AXXXXXXXXXXXXXXXXXETVAV 813
SLD RQ+S DGR+RNPDEKP +L + A QT A +T+
Sbjct: 367 PSLDGRQMSRDGRIRNPDEKP--ILAPAEPAPPQTEMAAKPNIKRTKEEPKPVPSDTLPA 424
Query: 812 ENAEKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+ +KE K K G L+ + E++ + +E K+ K VD AKL
Sbjct: 425 QKVDKEVKHKV-GKSARPLEQEDKAEDEIHGLEKLSKDIPKEPEVDLAKL 473
>gb|EOY13017.1| Proton pump interactor 1 isoform 2 [Theobroma cacao]
Length = 627
Score = 256 bits (654), Expect = 2e-65
Identities = 135/213 (63%), Positives = 172/213 (80%), Gaps = 1/213 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQL+REIKQLEGTR KVIANAAMRAKIQ+SLGQKEA+QD+VKL+G
Sbjct: 192 LQYRIQHESIPLSEEKQLLREIKQLEGTREKVIANAAMRAKIQDSLGQKEAIQDQVKLMG 251
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGV+KE+ + SK ++++++KAI+ I SLQ +LT+V +KRDKA ETI +LRKQRD
Sbjct: 252 VDLDGVRKEQQALWSKKNQIKEKVKAIESKIDSLQEELTAVTQKRDKAYETIQELRKQRD 311
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GNA F+Q+R+LLN KELAA+K+++AL+ELS EVEKFMA W K+FR DYEKRI S
Sbjct: 312 EGNAHFYQSRSLLNKAKELAAKKDIKALEELSIAEVEKFMALWRGKKAFRDDYEKRILPS 371
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQT 885
LD RQ+S DGR+RNPDEKPL + + +E +T
Sbjct: 372 LDSRQMSRDGRIRNPDEKPLVVPEAPLPSETET 404
>gb|EOY13016.1| Proton pump interactor 1 isoform 1 [Theobroma cacao]
Length = 636
Score = 256 bits (654), Expect = 2e-65
Identities = 135/213 (63%), Positives = 172/213 (80%), Gaps = 1/213 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQL+REIKQLEGTR KVIANAAMRAKIQ+SLGQKEA+QD+VKL+G
Sbjct: 192 LQYRIQHESIPLSEEKQLLREIKQLEGTREKVIANAAMRAKIQDSLGQKEAIQDQVKLMG 251
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGV+KE+ + SK ++++++KAI+ I SLQ +LT+V +KRDKA ETI +LRKQRD
Sbjct: 252 VDLDGVRKEQQALWSKKNQIKEKVKAIESKIDSLQEELTAVTQKRDKAYETIQELRKQRD 311
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GNA F+Q+R+LLN KELAA+K+++AL+ELS EVEKFMA W K+FR DYEKRI S
Sbjct: 312 EGNAHFYQSRSLLNKAKELAAKKDIKALEELSIAEVEKFMALWRGKKAFRDDYEKRILPS 371
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQT 885
LD RQ+S DGR+RNPDEKPL + + +E +T
Sbjct: 372 LDSRQMSRDGRIRNPDEKPLVVPEAPLPSETET 404
>ref|XP_006464761.1| PREDICTED: proton pump-interactor 1-like isoform X1 [Citrus sinensis]
gi|568820517|ref|XP_006464762.1| PREDICTED: proton
pump-interactor 1-like isoform X2 [Citrus sinensis]
gi|568820519|ref|XP_006464763.1| PREDICTED: proton
pump-interactor 1-like isoform X3 [Citrus sinensis]
Length = 607
Score = 256 bits (653), Expect = 2e-65
Identities = 144/287 (50%), Positives = 192/287 (66%), Gaps = 1/287 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E+I L EKQ++REIKQLEGTR KV+ANAAMRAKIQES+G+KE +QD+VKL+G
Sbjct: 186 LQYRIQHEIIPLSEEKQILREIKQLEGTREKVMANAAMRAKIQESMGKKEDIQDQVKLMG 245
Query: 1340 TNLDGVKKE-RVVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
++LDGVKKE + V +KI LE ++KA+D +I +LQ ++ V EKRDKA I +LRKQRD
Sbjct: 246 SDLDGVKKESQAVWAKISHLEGKVKALDEEIEALQQEVNDVAEKRDKAFANIKELRKQRD 305
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GNA FFQ R LLN K ++ +K+++ LKELS EVEK+M WN++K+FR DYEKR+ S
Sbjct: 306 EGNAYFFQYRALLNEAKAMSVKKDVQGLKELSNSEVEKYMTLWNNNKAFRDDYEKRLLQS 365
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGR+RNPDEKPL + + + +E + A +T+ +
Sbjct: 366 LDMRQLSRDGRIRNPDEKPLVVAETSVPSEPEIVAKPNVKQSKGDSKSASQKDTLPTQKV 425
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+ +K+ D P VVE P E VDEAKL
Sbjct: 426 QKEAAKKETEDTADFEIP---------VVEKPASE----KKVDEAKL 459
>ref|XP_006451833.1| hypothetical protein CICLE_v10008004mg [Citrus clementina]
gi|567919656|ref|XP_006451834.1| hypothetical protein
CICLE_v10008004mg [Citrus clementina]
gi|557555059|gb|ESR65073.1| hypothetical protein
CICLE_v10008004mg [Citrus clementina]
gi|557555060|gb|ESR65074.1| hypothetical protein
CICLE_v10008004mg [Citrus clementina]
Length = 520
Score = 256 bits (653), Expect = 2e-65
Identities = 144/287 (50%), Positives = 192/287 (66%), Gaps = 1/287 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E+I L EKQ++REIKQLEGTR KV+ANAAMRAKIQES+G+KE +QD+VKL+G
Sbjct: 186 LQYRIQHEIIPLSEEKQILREIKQLEGTREKVMANAAMRAKIQESMGKKEDIQDQVKLMG 245
Query: 1340 TNLDGVKKE-RVVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
++LDGVKKE + V +KI LE ++KA+D +I +LQ ++ V EKRDKA I +LRKQRD
Sbjct: 246 SDLDGVKKESQAVWAKISHLEGKVKALDEEIEALQQEVNDVAEKRDKAFANIKELRKQRD 305
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GNA FFQ R LLN K ++ +K+++ LKELS EVEK+M WN++K+FR DYEKR+ S
Sbjct: 306 EGNAYFFQYRALLNEAKAMSVKKDVQGLKELSNSEVEKYMTLWNNNKAFRDDYEKRLLQS 365
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGR+RNPDEKPL + + + +E + A +T+ +
Sbjct: 366 LDMRQLSRDGRIRNPDEKPLVVAETSVPSEPEIVAKPNVKQSKGDSKSASQKDTLPTQKV 425
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+ +K+ D P VVE P E VDEAKL
Sbjct: 426 QKEAAKKETEDTADFEIP---------VVEKPASE----KKVDEAKL 459
>ref|XP_003596760.1| hypothetical protein MTR_2g085330 [Medicago truncatula]
gi|355485808|gb|AES67011.1| hypothetical protein
MTR_2g085330 [Medicago truncatula]
Length = 600
Score = 252 bits (644), Expect = 3e-64
Identities = 150/289 (51%), Positives = 193/289 (66%), Gaps = 3/289 (1%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQ++REIKQLEGTR KVIAN+AMR K+ +++G K+ +QD+VKLIG
Sbjct: 182 LQYRIQHESIPLTEEKQILREIKQLEGTREKVIANSAMRTKLHDTIGPKDTIQDQVKLIG 241
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+++D LKAIDIDI SLQ +L +V +KR++A E+I KLRKQRD
Sbjct: 242 GDLDGVKKERQAIRSKIKQIDDVLKAIDIDIQSLQAELVAVTQKREQAFESIQKLRKQRD 301
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GN+ F+Q+RTLL +ELAA+K++ A+ ELS E EKFMA WN K+FR DYEKRI +S
Sbjct: 302 EGNSYFYQSRTLLTKARELAAKKDVAAIDELSQTEGEKFMALWNGGKAFRADYEKRILSS 361
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGR+RN DEKP LL+ K A + ET+ A
Sbjct: 362 LDMRQLSRDGRIRNWDEKP--LLEEPKPAAEPEALPKPVAKQPKEAPKPSPVETLP---A 416
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKE--TVKHDVVDEAKL 663
+KE+K K R A +D Y E P KE K +D AKL
Sbjct: 417 QKETKSKGRD---------LAAIDDDYEFENPHKEAPAAKEPEIDPAKL 456
>ref|XP_003596759.1| hypothetical protein MTR_2g085330 [Medicago truncatula]
gi|355485807|gb|AES67010.1| hypothetical protein
MTR_2g085330 [Medicago truncatula]
Length = 609
Score = 252 bits (644), Expect = 3e-64
Identities = 150/289 (51%), Positives = 193/289 (66%), Gaps = 3/289 (1%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQ++REIKQLEGTR KVIAN+AMR K+ +++G K+ +QD+VKLIG
Sbjct: 191 LQYRIQHESIPLTEEKQILREIKQLEGTREKVIANSAMRTKLHDTIGPKDTIQDQVKLIG 250
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+++D LKAIDIDI SLQ +L +V +KR++A E+I KLRKQRD
Sbjct: 251 GDLDGVKKERQAIRSKIKQIDDVLKAIDIDIQSLQAELVAVTQKREQAFESIQKLRKQRD 310
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GN+ F+Q+RTLL +ELAA+K++ A+ ELS E EKFMA WN K+FR DYEKRI +S
Sbjct: 311 EGNSYFYQSRTLLTKARELAAKKDVAAIDELSQTEGEKFMALWNGGKAFRADYEKRILSS 370
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGR+RN DEKP LL+ K A + ET+ A
Sbjct: 371 LDMRQLSRDGRIRNWDEKP--LLEEPKPAAEPEALPKPVAKQPKEAPKPSPVETLP---A 425
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKE--TVKHDVVDEAKL 663
+KE+K K R A +D Y E P KE K +D AKL
Sbjct: 426 QKETKSKGRD---------LAAIDDDYEFENPHKEAPAAKEPEIDPAKL 465
>ref|XP_002316898.2| hypothetical protein POPTR_0011s12040g [Populus trichocarpa]
gi|550328199|gb|EEE97510.2| hypothetical protein
POPTR_0011s12040g [Populus trichocarpa]
Length = 619
Score = 250 bits (638), Expect = 1e-63
Identities = 145/292 (49%), Positives = 194/292 (66%), Gaps = 6/292 (2%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YRMQ E I L EKQ++REIKQLEGTR KVIANAAMR KIQ+SLGQKEA+QD+VKL+G
Sbjct: 191 LQYRMQHESIPLTEEKQILREIKQLEGTREKVIANAAMRTKIQDSLGQKEAIQDQVKLMG 250
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGV+KER + K+ LE +++A+D +I +LQ +L +V++KR+K ET+ +LRKQRD
Sbjct: 251 VDLDGVRKERQALWEKLDGLEAKVQALDAEIKTLQDELEAVIQKREKTYETLQELRKQRD 310
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+ NA F+Q+R LL KELAA+K+++AL+EL+ EVEKFM+ W +KSFR DYEKRI S
Sbjct: 311 EANANFYQSRMLLTKAKELAAKKDVKALEELALDEVEKFMSLWCHNKSFRDDYEKRILPS 370
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD RQLS DGR+RNP+EKPL +L+ K +E + +T+ +
Sbjct: 371 LDRRQLSRDGRIRNPNEKPLVILETPKPSEPEPVVKAIPKRAKEDPKPIPPKDTLPTQRV 430
Query: 803 EKESKRKQRGDPKTILKPRN-----AEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE KT LKP + ++E + L K + VDEAKL
Sbjct: 431 QKEI-------TKTELKPTSEHSDIVDKEISGLENLSKNPSPAEKEVDEAKL 475
>gb|ACJ85701.1| unknown [Medicago truncatula]
Length = 577
Score = 249 bits (637), Expect = 2e-63
Identities = 149/289 (51%), Positives = 192/289 (66%), Gaps = 3/289 (1%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQ++REIKQLEGTR KVIAN+AMR K+ +++G K+ +QD+VK IG
Sbjct: 191 LQYRIQHESIPLTEEKQILREIKQLEGTREKVIANSAMRTKLHDTIGPKDTIQDQVKPIG 250
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGVKKER ++SKIK+++D LKAIDIDI SLQ +L +V +KR++A E+I KLRKQRD
Sbjct: 251 GDLDGVKKERQAIRSKIKQIDDVLKAIDIDIQSLQAELVAVTQKREQAFESIQKLRKQRD 310
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+GN+ F+Q+RTLL +ELAA+K++ A+ ELS E EKFMA WN K+FR DYEKRI +S
Sbjct: 311 EGNSYFYQSRTLLTKARELAAKKDVAAIDELSQTEGEKFMALWNGGKAFRADYEKRILSS 370
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD+RQLS DGR+RN DEKP LL+ K A + ET+ A
Sbjct: 371 LDMRQLSRDGRIRNWDEKP--LLEEPKPAAEPEALPKPAAKQPKEAPKPSPVETLP---A 425
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKE--TVKHDVVDEAKL 663
+KE+K K R A +D Y E P KE K +D AKL
Sbjct: 426 QKETKSKGRD---------LAAIDDDYEFENPHKEAPAAKEPEIDPAKL 465
>ref|XP_002329130.1| predicted protein [Populus trichocarpa]
Length = 616
Score = 247 bits (630), Expect = 1e-62
Identities = 140/287 (48%), Positives = 194/287 (67%), Gaps = 1/287 (0%)
Frame = -1
Query: 1520 LNYRMQREVISLPVEKQLMREIKQLEGTRAKVIANAAMRAKIQESLGQKEAMQDKVKLIG 1341
L YR+Q E I L EKQ++REIKQ EGTR KVIANAAMRAKIQ+S+GQKEA+QD+VKLIG
Sbjct: 191 LQYRIQHESIPLTEEKQILREIKQFEGTREKVIANAAMRAKIQDSMGQKEAIQDQVKLIG 250
Query: 1340 TNLDGVKKER-VVKSKIKELEDELKAIDIDIVSLQLDLTSVLEKRDKAVETIHKLRKQRD 1164
+LDGV+KE+ V+ K+ L+ ++KA+D +I +LQ +LT+V +KRDKA ETI +LRKQRD
Sbjct: 251 VDLDGVRKEQQVLWGKVDGLDGKVKALDAEIKTLQDELTAVTQKRDKAYETIQELRKQRD 310
Query: 1163 DGNACFFQNRTLLNNVKELAAEKNLEALKELSCKEVEKFMASWNSSKSFRKDYEKRISAS 984
+ NA F+Q R LL KELAA K++ AL+EL+ EVE FM+ W+ +K+FR DY+KRI S
Sbjct: 311 EANANFYQCRMLLTKAKELAARKDVPALEELAHAEVENFMSLWSHNKAFRDDYKKRILPS 370
Query: 983 LDIRQLSEDGRMRNPDEKPLFLLQHTKQAEGQTSAXXXXXXXXXXXXXXXXXETVAVENA 804
LD RQ+S+DGR+RNPDEKPL +L+ K +E + + + +
Sbjct: 371 LDRRQMSKDGRIRNPDEKPLVILETPKLSEPEPVVKAIAKRPKEDPKPPSQKDALPPQKV 430
Query: 803 EKESKRKQRGDPKTILKPRNAEEEDYYVVELPKKETVKHDVVDEAKL 663
+KE+ + + KT + + +++ +E +K VDEAKL
Sbjct: 431 QKETTKTEL---KTTSEHSDIIDKEVSGLENLQKNPSAEKEVDEAKL 474