BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00026368
         (531 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythra...   318   e-102
ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Eryt...   318   e-101
gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branchin...   311   4e-98
ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum...   301   2e-94
ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea...   296   6e-93
ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea...   296   9e-93
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] >gi...   280   4e-86
ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [...   280   4e-86
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]        279   4e-86
ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Sola...   273   7e-84
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             273   1e-83
ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Sola...   273   1e-83
ref|XP_019161030.1| PREDICTED: isoamylase 2, chloroplastic [Ipom...   273   1e-83
ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like ...   271   4e-83
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   270   1e-82
dbj|BAW33107.1| isoamylase [Ipomoea batatas]                          269   3e-82
ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like ...   268   8e-82
ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]        267   2e-81
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   266   3e-81
ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like ...   266   3e-81

>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythranthe guttata]
          Length = 747

 Score =  318 bits (814), Expect = e-102
 Identities = 149/176 (84%), Positives = 165/176 (93%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNLGVNA+LLEPIFPFDEQKGPYFPWHF
Sbjct: 257 LNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNAVLLEPIFPFDEQKGPYFPWHF 316

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVLLEVVFTHTSE+++LREIDNSSY+ V
Sbjct: 317 FSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVLLEVVFTHTSEISALREIDNSSYHHV 376

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFHIDGFCF+N+SSLTRG HGEFL
Sbjct: 377 EGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFHIDGFCFINSSSLTRGFHGEFL 432


>ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842326.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842327.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842328.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
          Length = 840

 Score =  318 bits (814), Expect = e-101
 Identities = 149/176 (84%), Positives = 165/176 (93%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNV RFT DKSSKLP DIAG+F+GIS+KLH+FKNLGVNA+LLEPIFPFDEQKGPYFPWHF
Sbjct: 350 LNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNAVLLEPIFPFDEQKGPYFPWHF 409

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPGSLYGPSGDP  VA S+KEMVKKLHA+G+EVLLEVVFTHTSE+++LREIDNSSY+ V
Sbjct: 410 FSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVLLEVVFTHTSEISALREIDNSSYHHV 469

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GGE+LKSRN+LNCNYPIVQQMILE LRYWV EFHIDGFCF+N+SSLTRG HGEFL
Sbjct: 470 EGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFHIDGFCFINSSSLTRGFHGEFL 525


>gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branching enzyme II
           [Handroanthus impetiginosus]
          Length = 853

 Score =  311 bits (796), Expect = 4e-98
 Identities = 149/176 (84%), Positives = 160/176 (90%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVTRFT   SSKLPGDIAG+F+G+SEKLHHFK+LGVNA+LLEPIFPFDE+K PYFP HF
Sbjct: 352 LNVTRFTRHNSSKLPGDIAGSFSGVSEKLHHFKDLGVNAVLLEPIFPFDEKKRPYFPAHF 411

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG+LYGPSG+P +VAKSMKEMVKKLHASGIEVLLEVVFTHT E  SLREIDNS YY +
Sbjct: 412 FSPGNLYGPSGNPNSVAKSMKEMVKKLHASGIEVLLEVVFTHTGETGSLREIDNSCYYHI 471

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GGEDLK  NALNCNYPIVQQMILESLRYWV EFHIDGFCF+NASSLTRG HGEFL
Sbjct: 472 KGGEDLKLGNALNCNYPIVQQMILESLRYWVTEFHIDGFCFINASSLTRGFHGEFL 527


>ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum]
 ref|XP_011092270.1| isoamylase 2, chloroplastic [Sesamum indicum]
          Length = 857

 Score =  301 bits (771), Expect = 2e-94
 Identities = 145/176 (82%), Positives = 158/176 (89%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LN+T FT +KSSKLPGDIAGTF+G+SEKLHHFK+LGVNAILLEPIFPFDE+KGPYFPWHF
Sbjct: 355 LNITGFTKNKSSKLPGDIAGTFSGLSEKLHHFKDLGVNAILLEPIFPFDEKKGPYFPWHF 414

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSP  L GP+GDP TVA+SMKEMVKKLH SGIEVLLEVVFTHT E  +LR+ID SSYY  
Sbjct: 415 FSPERLCGPTGDPTTVAQSMKEMVKKLHGSGIEVLLEVVFTHTGEGCALRDID-SSYYHT 473

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG+DL SRNALNCNYPIVQQM+LESLRYWVIEFHIDGFCF+NAS L RG HGEFL
Sbjct: 474 EGGDDLTSRNALNCNYPIVQQMVLESLRYWVIEFHIDGFCFINASHLMRGFHGEFL 529


>ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea europaea var.
           sylvestris]
          Length = 829

 Score =  296 bits (759), Expect = 6e-93
 Identities = 141/176 (80%), Positives = 161/176 (91%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNAILLEPIFPFDEQ GPYFPWHF
Sbjct: 327 LNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAILLEPIFPFDEQTGPYFPWHF 386

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFTHT+E+A+L++ID  SYY V
Sbjct: 387 FSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFTHTAEVAALKQIDR-SYYHV 445

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF++ASSL +G HGEFL
Sbjct: 446 GGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCFIDASSLMKGFHGEFL 501


>ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea europaea var.
           sylvestris]
          Length = 849

 Score =  296 bits (759), Expect = 9e-93
 Identities = 141/176 (80%), Positives = 161/176 (91%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVTRFT D+SSKLPG+I GTFAGISEK+HHFK+LGVNAILLEPIFPFDEQ GPYFPWHF
Sbjct: 347 LNVTRFTKDESSKLPGEIVGTFAGISEKVHHFKDLGVNAILLEPIFPFDEQTGPYFPWHF 406

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPGS YGPSGD + V K MKEMVKKLH +GIEVL+EVVFTHT+E+A+L++ID  SYY V
Sbjct: 407 FSPGSPYGPSGDSEFVVKLMKEMVKKLHLNGIEVLVEVVFTHTAEVAALKQIDR-SYYHV 465

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GGEDLKSRNALNCN+PIVQQ+IL+SLR+WVIE+HIDGFCF++ASSL +G HGEFL
Sbjct: 466 GGGEDLKSRNALNCNHPIVQQLILDSLRHWVIEYHIDGFCFIDASSLMKGFHGEFL 521


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum]
 gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  280 bits (715), Expect = 4e-86
 Identities = 135/176 (76%), Positives = 153/176 (86%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVT+FT DKSSKLP D+AGTF+GISEK HHFK+LGVNA+LLEPIFPFDEQKGPYFPWHF
Sbjct: 359 LNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWHF 418

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG++YGPSGDP +  KSMK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN S Y +
Sbjct: 419 FSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFS-YCI 477

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG+ L  +NALNCNYPIVQQMIL+ LR+WVIEFHIDGF FVNASSL RG +GE L
Sbjct: 478 KGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEIL 533


>ref|XP_006355876.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
 ref|XP_015168040.1| PREDICTED: isoamylase isoform 2 isoform X1 [Solanum tuberosum]
          Length = 878

 Score =  280 bits (715), Expect = 4e-86
 Identities = 135/176 (76%), Positives = 153/176 (86%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVT+FT DKSSKLP D+AGTF+GISEK HHFK+LGVNA+LLEPIFPFDEQKGPYFPWHF
Sbjct: 359 LNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWHF 418

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG++YGPSGDP +  KSMK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN S Y +
Sbjct: 419 FSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFS-YCI 477

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG+ L  +NALNCNYPIVQQMIL+ LR+WVIEFHIDGF FVNASSL RG +GE L
Sbjct: 478 KGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEIL 533


>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
          Length = 844

 Score =  279 bits (713), Expect = 4e-86
 Identities = 137/176 (77%), Positives = 150/176 (85%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVTRFT DKSSKLP   AGTF GI EKLHHFK+LGVNAILLEPIFP DE+K PYFPWHF
Sbjct: 344 LNVTRFTKDKSSKLPTSAAGTFYGIYEKLHHFKDLGVNAILLEPIFPSDERKEPYFPWHF 403

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPGS YGPS D   + KSMKEMVKKLHASGIEVLLEVVFTHT E  +L+ ID S Y+S+
Sbjct: 404 FSPGSSYGPSDDLSYIVKSMKEMVKKLHASGIEVLLEVVFTHTGEDVTLKGIDESYYHSI 463

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
              EDLKS+N LNCNYP+VQQ+ILESLR+WVIE+HIDGFCF+NASSL RG HGEFL
Sbjct: 464 ---EDLKSKNMLNCNYPVVQQLILESLRHWVIEYHIDGFCFINASSLLRGKHGEFL 516


>ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Solanum pennellii]
          Length = 866

 Score =  273 bits (699), Expect = 7e-84
 Identities = 131/176 (74%), Positives = 152/176 (86%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           +NVT FT DKSSKLP ++AGTF+GISEK HH K+LGVNA+LLEPIFPFDEQKGPY+PWHF
Sbjct: 359 INVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYPWHF 418

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG++YGPSGDP +V K+MK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN S Y +
Sbjct: 419 FSPGNMYGPSGDPLSVIKTMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFS-YCI 477

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG+DL  +NALNCN PIVQQMIL+ LR+WVIEFHIDGF FVNASSL RG +GE L
Sbjct: 478 KGGQDLNIQNALNCNCPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEIL 533


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  273 bits (698), Expect = 1e-83
 Identities = 130/176 (73%), Positives = 148/176 (84%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           +NV  FT  KSS LP D+AG+F GI+EKL H K+LGVNAILLEP+FPFDE+KGPYFP+HF
Sbjct: 359 VNVAHFTKHKSSGLPNDVAGSFTGITEKLQHCKDLGVNAILLEPVFPFDEKKGPYFPFHF 418

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSP  LYGPS DP     SMKEMVK LHA+GIEVLLE+VFTHT+E  SLREID SSYY +
Sbjct: 419 FSPMKLYGPSDDPSATVNSMKEMVKILHANGIEVLLEIVFTHTAESCSLREIDKSSYY-I 477

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            G EDL ++NALNCNYPIVQQMIL+SLRYW IEFH+DGFCF+NASSL +G HGEFL
Sbjct: 478 TGDEDLGAKNALNCNYPIVQQMILDSLRYWAIEFHVDGFCFINASSLLKGFHGEFL 533


>ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Solanum lycopersicum]
          Length = 866

 Score =  273 bits (698), Expect = 1e-83
 Identities = 130/176 (73%), Positives = 152/176 (86%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           +NVT FT DKSSKLP ++AGTF+GISEK HH K+LGVNA+LLEPIFPFDEQKGPY+PWHF
Sbjct: 359 INVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYPWHF 418

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG++YGPSGDP +V KSMK+MVKKLHA+GIEV LEVVFTHT+E A L  +DN S Y +
Sbjct: 419 FSPGNMYGPSGDPLSVIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFS-YCI 477

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            G +D+  +NALNCNYPIVQQMIL+ LR+WVIEFHIDGF FV+ASSL RG +GE L
Sbjct: 478 KGSQDMNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVDASSLLRGFNGEIL 533


>ref|XP_019161030.1| PREDICTED: isoamylase 2, chloroplastic [Ipomoea nil]
          Length = 865

 Score =  273 bits (697), Expect = 1e-83
 Identities = 130/176 (73%), Positives = 149/176 (84%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNV  FT DKSSKLP  +AGTF+GI+EKL HFK++GVNAILLEPIFPFDEQKGPYFP HF
Sbjct: 361 LNVCNFTKDKSSKLPAQVAGTFSGITEKLQHFKDIGVNAILLEPIFPFDEQKGPYFPMHF 420

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG L GP GDP +V  SMK+MVK++HA+GIEV L+VVFTHT+E ASL  ID SSYY +
Sbjct: 421 FSPGHLCGPPGDPSSVINSMKQMVKEVHANGIEVFLQVVFTHTAEGASLLHIDKSSYYYL 480

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            G   +KS N+LNCNYP VQQMIL+SLR+WV+E+HIDGFCFVNAS LTRG HGEFL
Sbjct: 481 EGDMGMKSGNSLNCNYPPVQQMILDSLRHWVVEYHIDGFCFVNASFLTRGFHGEFL 536


>ref|XP_016450982.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score =  271 bits (694), Expect = 4e-83
 Identities = 132/176 (75%), Positives = 151/176 (85%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNAILLEPIFPF+EQKGPYFP HF
Sbjct: 360 LNVTEFTNDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFPSHF 419

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG+++G SGDP +V KSMK+MVKKLHA+GIE+ LEVVFTHT+E A L  +DNSSYYS+
Sbjct: 420 FSPGNMHGHSGDPLSVIKSMKDMVKKLHANGIEIFLEVVFTHTAEDAPLMHVDNSSYYSI 479

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF F+NASSL +G HGE L
Sbjct: 480 KGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFINASSLLKGFHGEIL 534


>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
 ref|XP_018625653.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
          Length = 863

 Score =  270 bits (690), Expect = 1e-82
 Identities = 131/174 (75%), Positives = 150/174 (86%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNAILLEPIFPF+EQKGPYFP HF
Sbjct: 360 LNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFPSHF 419

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG+++G SGDP +V KSMK+MVKKLHA+GIE+ LEVVFTHT+E A L  +DNSSYYS+
Sbjct: 420 FSPGNMHGRSGDPLSVIKSMKDMVKKLHANGIEIFLEVVFTHTAEDAPLMHVDNSSYYSI 479

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGE 9
            GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF F+NASSL +G HGE
Sbjct: 480 KGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFINASSLLKGFHGE 532


>dbj|BAW33107.1| isoamylase [Ipomoea batatas]
          Length = 865

 Score =  269 bits (688), Expect = 3e-82
 Identities = 128/176 (72%), Positives = 149/176 (84%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNV+ FT DKSSKLP  +AGTF+GI+EKL HFK+LGVNAILLEPIFPFDEQKGPYFP HF
Sbjct: 361 LNVSNFTKDKSSKLPAQVAGTFSGITEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPVHF 420

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG L GP GDP ++  SMK+MVK++HA+GIEV L+VVFTHT+E ASL  ID SSYY +
Sbjct: 421 FSPGHLCGPPGDPSSIINSMKQMVKEVHANGIEVFLQVVFTHTAEGASLLHIDKSSYYYL 480

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
                +KS N+LNCNYP VQQ+IL+SLR+WV+E+HIDGFCFVNAS LTRG HGEFL
Sbjct: 481 EDDMGMKSGNSLNCNYPPVQQLILDSLRHWVVEYHIDGFCFVNASFLTRGFHGEFL 536


>ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
          Length = 870

 Score =  268 bits (685), Expect = 8e-82
 Identities = 127/176 (72%), Positives = 148/176 (84%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVTRFT  KSS LP +IAGTF+G++EKL H K+LGVNA+LLEPIFPFDEQKGPYFP HF
Sbjct: 363 LNVTRFTEHKSSNLPTNIAGTFSGLTEKLEHLKHLGVNAVLLEPIFPFDEQKGPYFPIHF 422

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSP + +GPS  P +   SMK+MVKK HA GIEVLLEVVFTHT+E  +L+ ID SSYY +
Sbjct: 423 FSPMNWFGPSRGPVSAVNSMKDMVKKFHADGIEVLLEVVFTHTAEGEALQGIDISSYYHI 482

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
           NG  DLK+RNALNCNYP+VQQM+L+SLRYWV EFH+DGFCF+NASSL RG  GE+L
Sbjct: 483 NGVADLKARNALNCNYPVVQQMVLDSLRYWVTEFHVDGFCFINASSLMRGSKGEYL 538


>ref|XP_021805716.1| isoamylase 2, chloroplastic [Prunus avium]
          Length = 883

 Score =  267 bits (683), Expect = 2e-81
 Identities = 126/176 (71%), Positives = 150/176 (85%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNV RFT  KSS+LP DI G+F+G++EKL HFK+LGVNA+LLEPIFPFDEQKGPYFP HF
Sbjct: 376 LNVMRFTEHKSSQLPTDIGGSFSGLTEKLEHFKDLGVNAVLLEPIFPFDEQKGPYFPRHF 435

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSP   +GPSG P +   SMKEMV+K HA+GIEVLLEVVFTHT+E  +L+ ID SSYY V
Sbjct: 436 FSPMDCFGPSGGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAEGEALQGIDISSYYHV 495

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
           N  EDL++RNALNCNYP+VQQ++L+SLRYWV EFH+DGFCF+NASSL RG +GE+L
Sbjct: 496 NEVEDLEARNALNCNYPVVQQLVLDSLRYWVTEFHVDGFCFINASSLLRGFNGEYL 551


>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
 gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
          Length = 863

 Score =  266 bits (681), Expect = 3e-81
 Identities = 130/176 (73%), Positives = 150/176 (85%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVT FT D SSKLP D+AGTF+GI+EKL HFK+LGVNAILLEPIFPF+EQKGPYFP HF
Sbjct: 360 LNVTEFTKDGSSKLPDDLAGTFSGITEKLSHFKHLGVNAILLEPIFPFEEQKGPYFPSHF 419

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG+++G S DP +V  SMK+MVKKLHA+GIE+ LEVVFTH +E A L  +DNSS+YS+
Sbjct: 420 FSPGNMHGRSSDPLSVINSMKDMVKKLHANGIEIFLEVVFTHIAEDAPLMHVDNSSHYSI 479

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG DL  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF FVNASSL++G HGE L
Sbjct: 480 KGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFVNASSLSKGFHGEIL 534


>ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
 ref|XP_016475508.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score =  266 bits (681), Expect = 3e-81
 Identities = 130/176 (73%), Positives = 149/176 (84%)
 Frame = -2

Query: 530 LNVTRFTIDKSSKLPGDIAGTFAGISEKLHHFKNLGVNAILLEPIFPFDEQKGPYFPWHF 351
           LNVT FT D SSKLP D+AGTF+GI+EK  HFK+LGVNAILLEPIFPF+EQKGPYFP HF
Sbjct: 360 LNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFPSHF 419

Query: 350 FSPGSLYGPSGDPKTVAKSMKEMVKKLHASGIEVLLEVVFTHTSELASLREIDNSSYYSV 171
           FSPG+++G S DP +V KSMK+MVKKLHA+GIE+ LEV+FTHT+E A L  IDNS YYS+
Sbjct: 420 FSPGNMHGRSSDPLSVIKSMKDMVKKLHANGIEIFLEVIFTHTAEDAPLMHIDNSCYYSI 479

Query: 170 NGGEDLKSRNALNCNYPIVQQMILESLRYWVIEFHIDGFCFVNASSLTRGGHGEFL 3
            GG D+  + ALNCNYPIVQQMIL+ LRYWVIEFHIDGF FVNASSL +G HGE L
Sbjct: 480 KGG-DMNIQFALNCNYPIVQQMILDCLRYWVIEFHIDGFVFVNASSLLKGFHGEIL 534