BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00023669
         (761 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011100321.1| cytochrome P450 81D1-like [Sesamum indicum]       347   e-115
gb|PIN04635.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   308   1e-99
ref|XP_012852360.1| PREDICTED: cytochrome P450 81D1-like [Erythr...   309   1e-99
ref|XP_022872978.1| isoflavone 2'-hydroxylase-like [Olea europae...   295   2e-94
gb|EYU41656.1| hypothetical protein MIMGU_mgv1a026579mg [Erythra...   291   1e-92
gb|PHU20730.1| hypothetical protein BC332_11881 [Capsicum chinense]   290   2e-92
ref|XP_016568838.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   290   2e-92
ref|XP_009620154.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   290   4e-92
ref|XP_019262546.1| PREDICTED: isoflavone 2'-hydroxylase-like [N...   288   2e-91
gb|PHT51403.1| hypothetical protein CQW23_11150 [Capsicum baccatum]   287   4e-91
gb|KZV35074.1| hypothetical protein F511_04379 [Dorcoceras hygro...   282   4e-91
ref|XP_009796226.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   286   1e-90
ref|XP_018631766.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   285   2e-90
ref|XP_012831740.1| PREDICTED: cytochrome P450 81E8-like [Erythr...   285   4e-90
ref|XP_021612036.1| cytochrome P450 81E8-like [Manihot esculenta...   283   8e-90
gb|EEF52317.1| cytochrome P450, putative [Ricinus communis]           283   1e-89
ref|XP_010271541.1| PREDICTED: isoflavone 3'-hydroxylase-like [N...   282   3e-89
ref|XP_006362076.1| PREDICTED: cytochrome P450 81D1-like [Solanu...   282   3e-89
ref|XP_009796227.1| PREDICTED: isoflavone 2'-hydroxylase-like is...   281   5e-89
ref|XP_004238115.2| PREDICTED: cytochrome P450 81D1-like [Solanu...   288   7e-89

>ref|XP_011100321.1| cytochrome P450 81D1-like [Sesamum indicum]
          Length = 499

 Score =  347 bits (890), Expect = e-115
 Identities = 173/228 (75%), Positives = 191/228 (83%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           LIE L DLQR EPEYYTDETIRNL LVLVQGASHTSS TLEWAFSLLLQ+PDILT+AR+E
Sbjct: 281 LIEVLLDLQREEPEYYTDETIRNLFLVLVQGASHTSSNTLEWAFSLLLQHPDILTRARAE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           I         I +SD++KIPYL  IINETLRMHPAAPLLTPH SS  CIVGGF VPRGT+
Sbjct: 341 I---------IRDSDITKIPYLRGIINETLRMHPAAPLLTPHSSSRTCIVGGFHVPRGTI 391

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAWDIQN+P+ W+DPEKF PERFEGLE GKN+FK         GCPGEN+A  MVGL
Sbjct: 392 LLVNAWDIQNDPRTWDDPEKFVPERFEGLEEGKNDFKYLPFGWGRRGCPGENMAKQMVGL 451

Query: 199 ALGSLIQCFEWEKVGEIDMREGKGIITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSLIQCFEWE +GEIDM EGKG+ITP+VQPLRA+CIPRPFVV+L+S
Sbjct: 452 ALGSLIQCFEWESIGEIDMSEGKGVITPKVQPLRARCIPRPFVVDLLS 499


>gb|PIN04635.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 485

 Score =  308 bits (789), Expect = 1e-99
 Identities = 152/228 (66%), Positives = 176/228 (77%), Gaps = 1/228 (0%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           LI+ L DL+R +PEYYTDETIRNLLLVLVQGASH++S TLEWAFSLLL+NPD +T+AR+E
Sbjct: 277 LIKVLLDLERGQPEYYTDETIRNLLLVLVQGASHSTSNTLEWAFSLLLENPDTMTRARAE 336

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+LVG+                    +TLRMHPAAPLLTPH SS++CIVGGF VP GTM
Sbjct: 337 IDNLVGKE-------------------QTLRMHPAAPLLTPHYSSEKCIVGGFSVPPGTM 377

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKN-EFKXXXXXXXXXGCPGENLAMHMVG 203
           LLVN WD QNNPQ W DPEKF P+RF G    K+  FK          CPGENLAM+MVG
Sbjct: 378 LLVNVWDFQNNPQTWKDPEKFMPDRFTGKNSYKDYNFKFVPFGRGRRACPGENLAMNMVG 437

Query: 202 LALGSLIQCFEWEKVGEIDMREGKGIITPRVQPLRAKCIPRPFVVNLV 59
           LALG+LIQCFEWEK GEIDM EG+G+ITPR+QPLRAKCIPRPF+V+L+
Sbjct: 438 LALGTLIQCFEWEKYGEIDMGEGRGVITPRIQPLRAKCIPRPFLVDLL 485


>ref|XP_012852360.1| PREDICTED: cytochrome P450 81D1-like [Erythranthe guttata]
 gb|EYU24831.1| hypothetical protein MIMGU_mgv1a022968mg [Erythranthe guttata]
          Length = 515

 Score =  309 bits (791), Expect = 1e-99
 Identities = 158/230 (68%), Positives = 183/230 (79%), Gaps = 5/230 (2%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           LI+ L DLQR EPEYYTD+TIRNLLLVL+QGA+H +S TLEWAFS LLQNP ILT+AR  
Sbjct: 280 LIQFLLDLQREEPEYYTDQTIRNLLLVLLQGATHATSATLEWAFSHLLQNPHILTRARFH 339

Query: 559 IDS-LVGEHR-LITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRG 386
           ID+ L+G+ R LITES+V++IPYL  IINETLRMHP APLLTPHLSS EC +GGF VPRG
Sbjct: 340 IDNHLIGKERHLITESEVAQIPYLHLIINETLRMHPPAPLLTPHLSSQECTIGGFRVPRG 399

Query: 385 TMLLVNAWDIQNNPQYWNDPEKFKPERFEGL-EGGKNEFKXXXXXXXXXGCPGENLAMHM 209
           TMLLVNAWDI NNP+ W +P +F PERFEG+ +  KN F          GCPGE LA+ M
Sbjct: 400 TMLLVNAWDIHNNPETWENPNEFLPERFEGVGKNNKNGFDFLPFGWGRRGCPGEKLAVAM 459

Query: 208 VGLALGSLIQCFEWEKV--GEIDMREGKGIITPRVQPLRAKCIPRPFVVN 65
           +G  LG LIQCFEWE+V  GEIDMREGKG+IT RVQPL+AKC PR F+V+
Sbjct: 460 IGAVLGCLIQCFEWERVGGGEIDMREGKGVITTRVQPLKAKCCPRSFLVD 509


>ref|XP_022872978.1| isoflavone 2'-hydroxylase-like [Olea europaea var. sylvestris]
          Length = 500

 Score =  295 bits (756), Expect = 2e-94
 Identities = 143/227 (62%), Positives = 174/227 (76%)
 Frame = -2

Query: 736 IEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSEI 557
           ++ L DLQR EPEYY+DETIRNLLLVLV GA+HT+S T+EWAFS LL NP IL +A+ +I
Sbjct: 271 LQILLDLQREEPEYYSDETIRNLLLVLVHGATHTTSVTMEWAFSHLLDNPYILRRAQDDI 330

Query: 556 DSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTML 377
           D+LVG+ RLI ESD++++PYL  I+NETLR+HP  PLL PH+SS EC VGGF VP GT+L
Sbjct: 331 DTLVGKERLIDESDIAELPYLRCIVNETLRLHPPLPLLLPHVSSGECTVGGFRVPLGTLL 390

Query: 376 LVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGLA 197
            VN W +QN+ + W D EKFKPERFEG   GK+E K          CPGENLAM +V LA
Sbjct: 391 FVNVWAVQNDDEKWVDAEKFKPERFEGFR-GKDELKFFPFGWGRRACPGENLAMRVVELA 449

Query: 196 LGSLIQCFEWEKVGEIDMREGKGIITPRVQPLRAKCIPRPFVVNLVS 56
           LGSLIQCFEWEK+G+IDM E KGI+T ++  L AKC PRP V NL++
Sbjct: 450 LGSLIQCFEWEKIGKIDMSEDKGIVTSKIHSLIAKCNPRPSVYNLIN 496


>gb|EYU41656.1| hypothetical protein MIMGU_mgv1a026579mg [Erythranthe guttata]
          Length = 523

 Score =  291 bits (745), Expect = 1e-92
 Identities = 138/229 (60%), Positives = 172/229 (75%), Gaps = 1/229 (0%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQR+EP+YYTDETI+NLLLVL+QG S TS+  LEWAFSLLL NP+ L KA+SE
Sbjct: 295 MVEVLLDLQRSEPDYYTDETIKNLLLVLLQGGSSTSTIALEWAFSLLLDNPETLKKAQSE 354

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG  RLITE+DV+ +PYL +++ ETLR+HP   +L PH SS EC VGGF +P  T+
Sbjct: 355 IDARVGFDRLITETDVAALPYLRHVVLETLRLHPPVSILMPHRSSAECTVGGFRIPANTI 414

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKN-EFKXXXXXXXXXGCPGENLAMHMVG 203
           LLVN WDI +NP+ W DPE+F+PERFEG +G K   F+          CPGENL +  +G
Sbjct: 415 LLVNIWDIHHNPEIWPDPEEFRPERFEGFDGKKELGFRFFPFGSGRRACPGENLGISNIG 474

Query: 202 LALGSLIQCFEWEKVGEIDMREGKGIITPRVQPLRAKCIPRPFVVNLVS 56
           L LGSLIQCF+WEK+GEIDM EG G  TP+VQPL A+C PR F+ N +S
Sbjct: 475 LGLGSLIQCFDWEKIGEIDMSEGAGTTTPKVQPLTARCKPRHFLENFIS 523


>gb|PHU20730.1| hypothetical protein BC332_11881 [Capsicum chinense]
          Length = 506

 Score =  290 bits (742), Expect = 2e-92
 Identities = 144/231 (62%), Positives = 177/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL NP+IL KA++E
Sbjct: 274 IMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLDNPEILKKAQTE 333

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL PHLSS+EC V G+ VPRGT+
Sbjct: 334 IDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLVPHLSSEECKVAGYRVPRGTV 393

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G E  K   K         GCPGENLA+H++GL
Sbjct: 394 LLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIPFGSGRRGCPGENLAIHVIGL 453

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEW+K     IDM EG G  ++P+VQPL AKC PRP +V L+S
Sbjct: 454 ALGSLLQCFEWDKPNREIIDMSEGTGFTLSPKVQPLLAKCSPRPHMVKLLS 504


>ref|XP_016568838.1| PREDICTED: isoflavone 2'-hydroxylase-like [Capsicum annuum]
          Length = 506

 Score =  290 bits (742), Expect = 2e-92
 Identities = 144/231 (62%), Positives = 177/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL NP+IL KA++E
Sbjct: 274 IMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLDNPEILKKAQTE 333

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL PHLSS+EC V G+ VPRGT+
Sbjct: 334 IDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLVPHLSSEECKVAGYRVPRGTV 393

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G E  K   K         GCPGENLA+H++GL
Sbjct: 394 LLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIPFGSGRRGCPGENLAIHVIGL 453

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEW+K     IDM EG G  ++P+VQPL AKC PRP +V L+S
Sbjct: 454 ALGSLLQCFEWDKPNREIIDMSEGTGFTLSPKVQPLLAKCSPRPHMVKLLS 504


>ref|XP_009620154.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Nicotiana
           tomentosiformis]
 ref|XP_016477062.1| PREDICTED: isoflavone 2'-hydroxylase-like [Nicotiana tabacum]
          Length = 513

 Score =  290 bits (741), Expect = 4e-92
 Identities = 146/231 (63%), Positives = 176/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNLLLVL+Q  S TS+ TLEWAFS LL N +IL KA++E
Sbjct: 281 IMEVLLDLQKTDPEYYTDETIRNLLLVLLQAGSDTSAVTLEWAFSHLLDNLEILKKAQAE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS EC V G+ VPRGT+
Sbjct: 341 IDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSKECKVAGYRVPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G EG K   K         GCPGENLA H++GL
Sbjct: 401 LLVNAWGIHHDPKVWEDPEKFNPDRFIGFEGVKEGCKFIPFGSGRRGCPGENLAFHVIGL 460

Query: 199 ALGSLIQCFEWEKV--GEIDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEWEK   G IDM EG G  ++P+VQPL AKC PRP +V L+S
Sbjct: 461 ALGSLLQCFEWEKPNRGIIDMSEGTGFTLSPKVQPLLAKCSPRPNMVKLLS 511


>ref|XP_019262546.1| PREDICTED: isoflavone 2'-hydroxylase-like [Nicotiana attenuata]
 gb|OIT37725.1| cytochrome p450 81d1 [Nicotiana attenuata]
          Length = 513

 Score =  288 bits (737), Expect = 2e-91
 Identities = 143/231 (61%), Positives = 176/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +P YYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL N +IL KA++E
Sbjct: 281 IMEVLLDLQKTDPGYYTDETIRNLMLVLLQAGSETSAVTLEWAFSHLLDNLEILKKAQAE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS+EC V G+ +PRGT+
Sbjct: 341 IDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSEECKVAGYRIPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF PERF G EG K   K         GCPGENLA H++GL
Sbjct: 401 LLVNAWGIHHDPKVWEDPEKFNPERFIGFEGIKEGCKFIPFGSGRRGCPGENLAFHVIGL 460

Query: 199 ALGSLIQCFEWEKV--GEIDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEWEK   G IDM EG G  ++P+VQPL AKC PRP +V L++
Sbjct: 461 ALGSLLQCFEWEKPNRGSIDMSEGTGFTLSPKVQPLLAKCSPRPNMVKLLA 511


>gb|PHT51403.1| hypothetical protein CQW23_11150 [Capsicum baccatum]
          Length = 507

 Score =  287 bits (734), Expect = 4e-91
 Identities = 143/231 (61%), Positives = 176/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL NP+IL KA++E
Sbjct: 275 IMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLDNPEILRKAQTE 334

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD+ ++PY+  IINETLRMHPAAPLL PHLSS+EC V G+ VPRGT+
Sbjct: 335 IDNHVGQDRLIDESDLPQLPYIRCIINETLRMHPAAPLLVPHLSSEECKVAGYRVPRGTV 394

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G E  K   K         GCPGENLA+H++GL
Sbjct: 395 LLVNAWGIHHDPKVWADPEKFNPDRFIGFECFKESCKFIPFGSGRRGCPGENLAIHIIGL 454

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEW+K     IDM EG G  ++P+VQPL AKC  RP +V L+S
Sbjct: 455 ALGSLLQCFEWDKPNREIIDMSEGTGFTLSPKVQPLLAKCSTRPHMVKLLS 505


>gb|KZV35074.1| hypothetical protein F511_04379 [Dorcoceras hygrometricum]
          Length = 370

 Score =  282 bits (722), Expect = 4e-91
 Identities = 136/228 (59%), Positives = 170/228 (74%), Gaps = 1/228 (0%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQR+EP+YYTDETI++LLLVL+QG S TS+  LEWAFSLLL NP +L KA+SE
Sbjct: 142 MVEVLLDLQRSEPKYYTDETIKSLLLVLLQGGSSTSAIALEWAFSLLLDNPQVLKKAQSE 201

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG  RLITESDV+ +PYL  II ET R+HP   ++ PH SS  C VGG+ +P GT+
Sbjct: 202 IDAHVGTDRLITESDVAALPYLRRIILETFRLHPPVSIIMPHRSSTACTVGGYRIPAGTI 261

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKN-EFKXXXXXXXXXGCPGENLAMHMVG 203
           LLVN W+I NNP+ W DPEKF+P+RF GL+  K+  F+          CPGE LA+  +G
Sbjct: 262 LLVNTWEIHNNPKTWADPEKFRPDRFLGLDCKKHLGFRIVPFGWGRRACPGEKLAISSIG 321

Query: 202 LALGSLIQCFEWEKVGEIDMREGKGIITPRVQPLRAKCIPRPFVVNLV 59
           L LGSLIQCF+W+ VG+IDM EG G+ TP+VQPL AKC PRPFV  +V
Sbjct: 322 LGLGSLIQCFDWKTVGKIDMSEGLGVTTPKVQPLLAKCTPRPFVKKIV 369


>ref|XP_009796226.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Nicotiana
           sylvestris]
 ref|XP_016503270.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X1 [Nicotiana
           tabacum]
          Length = 513

 Score =  286 bits (731), Expect = 1e-90
 Identities = 144/231 (62%), Positives = 176/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL N +IL KA++E
Sbjct: 281 IMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLDNLEILKKAQAE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++ Y+  IINETLRMHPAAPLL PHLSS+EC V  + VPRGT+
Sbjct: 341 IDNHVGQDRLIDESDLAQLHYIRCIINETLRMHPAAPLLVPHLSSEECNVASYRVPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G EG K   K         GCPGENLA H++GL
Sbjct: 401 LLVNAWGIHHDPKVWEDPEKFYPDRFIGFEGIKEGCKFIPFGSGRRGCPGENLAFHVIGL 460

Query: 199 ALGSLIQCFEWEKV--GEIDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEWEK   G IDM EG G  ++P+VQPL AKC PRP +V L+S
Sbjct: 461 ALGSLLQCFEWEKPNRGSIDMSEGTGFTLSPKVQPLLAKCSPRPNMVKLLS 511


>ref|XP_018631766.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X2 [Nicotiana
           tomentosiformis]
          Length = 508

 Score =  285 bits (730), Expect = 2e-90
 Identities = 143/224 (63%), Positives = 171/224 (76%), Gaps = 3/224 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNLLLVL+Q  S TS+ TLEWAFS LL N +IL KA++E
Sbjct: 281 IMEVLLDLQKTDPEYYTDETIRNLLLVLLQAGSDTSAVTLEWAFSHLLDNLEILKKAQAE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PH SS EC V G+ VPRGT+
Sbjct: 341 IDNHVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHFSSKECKVAGYRVPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G EG K   K         GCPGENLA H++GL
Sbjct: 401 LLVNAWGIHHDPKVWEDPEKFNPDRFIGFEGVKEGCKFIPFGSGRRGCPGENLAFHVIGL 460

Query: 199 ALGSLIQCFEWEKV--GEIDMREGKGI-ITPRVQPLRAKCIPRP 77
           ALGSL+QCFEWEK   G IDM EG G  ++P+VQPL AKC PRP
Sbjct: 461 ALGSLLQCFEWEKPNRGIIDMSEGTGFTLSPKVQPLLAKCSPRP 504


>ref|XP_012831740.1| PREDICTED: cytochrome P450 81E8-like [Erythranthe guttata]
          Length = 528

 Score =  285 bits (729), Expect = 4e-90
 Identities = 138/234 (58%), Positives = 172/234 (73%), Gaps = 6/234 (2%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLV-----LVQGASHTSSTTLEWAFSLLLQNPDILT 575
           ++E L DLQR+EP+YYTDETI+NLLLV     L+QG S TS+  LEWAFSLLL NP+ L 
Sbjct: 295 MVEVLLDLQRSEPDYYTDETIKNLLLVRLFQVLLQGGSSTSTIALEWAFSLLLDNPETLK 354

Query: 574 KARSEIDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCV 395
           KA+SEID+ VG  RLITE+DV+ +PYL +++ ETLR+HP   +L PH SS EC VGGF +
Sbjct: 355 KAQSEIDARVGFDRLITETDVAALPYLRHVVLETLRLHPPVSILMPHRSSAECTVGGFRI 414

Query: 394 PRGTMLLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKN-EFKXXXXXXXXXGCPGENLA 218
           P  T+LLVN WDI +NP+ W DPE+F+PERFEG +G K   F+          CPGENL 
Sbjct: 415 PANTILLVNIWDIHHNPEIWPDPEEFRPERFEGFDGKKELGFRFFPFGSGRRACPGENLG 474

Query: 217 MHMVGLALGSLIQCFEWEKVGEIDMREGKGIITPRVQPLRAKCIPRPFVVNLVS 56
           +  +GL LGSLIQCF+WEK+GEIDM EG G  TP+VQPL A+C PR F+ N +S
Sbjct: 475 ISNIGLGLGSLIQCFDWEKIGEIDMSEGAGTTTPKVQPLTARCKPRHFLENFIS 528


>ref|XP_021612036.1| cytochrome P450 81E8-like [Manihot esculenta]
 gb|OAY50994.1| hypothetical protein MANES_05G179100 [Manihot esculenta]
          Length = 495

 Score =  283 bits (724), Expect = 8e-90
 Identities = 136/231 (58%), Positives = 174/231 (75%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           LIE L  LQ+++PEYYTD+TI+NL+LVL+   + TS+ T+EWA SLL+ NP+IL KA+ E
Sbjct: 263 LIEVLLTLQQSDPEYYTDQTIKNLMLVLLGAGTDTSAATMEWAMSLLVNNPEILKKAQKE 322

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID ++G +RLITESD SKIPYL  II+E LRM+PA PLL PH SS+EC VGG+ VP GTM
Sbjct: 323 IDIVIGHNRLITESDTSKIPYLQCIISEVLRMYPAGPLLVPHESSEECSVGGYRVPPGTM 382

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLE-GGKNEFKXXXXXXXXXGCPGENLAMHMVG 203
           LL N W IQN+PQ W++P+KFKPERF+G E G ++ F+          CPGE LA+ MVG
Sbjct: 383 LLANIWSIQNDPQVWDEPQKFKPERFDGREVGVRDGFRLMPFGSGRRSCPGEGLALRMVG 442

Query: 202 LALGSLIQCFEWEKVGE--IDMREGKGIITPRVQPLRAKCIPRPFVVNLVS 56
           L LGS++ CFEWE+VG+  +DM EG G+  P+ QPL  KC PRP +VNL+S
Sbjct: 443 LTLGSILHCFEWERVGKEIVDMTEGVGLTMPKAQPLLVKCRPRPSIVNLLS 493


>gb|EEF52317.1| cytochrome P450, putative [Ricinus communis]
          Length = 500

 Score =  283 bits (723), Expect = 1e-89
 Identities = 132/230 (57%), Positives = 171/230 (74%), Gaps = 2/230 (0%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           +IE L  LQ +EPEYYTDETI++L+LVL+   + TS+ T+EWA SLL+ NP+ L KA++E
Sbjct: 269 MIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTSAATMEWAMSLLVNNPEALKKAQTE 328

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           IDS++G  RLI ESD SK+PYL  IINE +RM+PA PLL PH SS+EC +GG+ VP GTM
Sbjct: 329 IDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAGPLLVPHESSEECFIGGYRVPAGTM 388

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVN W IQN+P+ W +P  FKPERFEG EG ++ F+          CPGE LA+ MVGL
Sbjct: 389 LLVNLWSIQNDPRVWEEPRNFKPERFEGCEGVRDGFRLMPFGSGRRSCPGEGLALRMVGL 448

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGIITPRVQPLRAKCIPRPFVVNLVS 56
            +G+L+QCF+WE+VG+  IDM EG G+  P+ QPL  +C PRP +VNL+S
Sbjct: 449 GIGTLLQCFDWERVGKEMIDMTEGVGLTMPKAQPLVVQCSPRPSMVNLLS 498


>ref|XP_010271541.1| PREDICTED: isoflavone 3'-hydroxylase-like [Nelumbo nucifera]
          Length = 512

 Score =  282 bits (722), Expect = 3e-89
 Identities = 137/231 (59%), Positives = 177/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           +I+ L  LQ+AEPEYYTD+ I+ L+L L+   + TS+ T+EWA SLLL NPD+L KA+ E
Sbjct: 280 MIDVLLSLQKAEPEYYTDKIIKGLVLGLLSAGTDTSAGTMEWAMSLLLNNPDVLKKAQDE 339

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           +D+ VG+ RL+ ESD+SK+PYL  IINETLRM+PA PLL PH SS++C++GGF +PRGTM
Sbjct: 340 VDNHVGQGRLLDESDLSKLPYLRCIINETLRMYPAGPLLVPHESSEDCVIGGFDIPRGTM 399

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVN W I N+P+ W +P KF+PERFEGLEG ++ FK         GCPGE LA  +VGL
Sbjct: 400 LLVNLWAIHNDPKIWEEPGKFEPERFEGLEGRRDGFKLMPFGSGRRGCPGEGLAERVVGL 459

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGIIT-PRVQPLRAKCIPRPFVVNLVS 56
           ALGSLIQCFEWE+VG+  +DM EG G +T P+ Q L+AKC PRP ++NL+S
Sbjct: 460 ALGSLIQCFEWERVGDDLVDMTEGAGGLTLPKAQALKAKCKPRPKMMNLLS 510


>ref|XP_006362076.1| PREDICTED: cytochrome P450 81D1-like [Solanum tuberosum]
          Length = 513

 Score =  282 bits (722), Expect = 3e-89
 Identities = 140/231 (60%), Positives = 176/231 (76%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIR+L+LVL+Q  S TS+ TLEWAFS LL NP+IL  A++E
Sbjct: 281 IMEVLLDLQKTDPEYYTDETIRSLMLVLLQAGSDTSAVTLEWAFSHLLDNPEILKTAQTE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+EC V G+ VPRGT+
Sbjct: 341 IDNQVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHLSSEECKVAGYRVPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W +P+KF P RF G EG K   K         GCPGENLA+H++GL
Sbjct: 401 LLVNAWGIHHDPKVWEEPQKFNPGRFLGFEGVKEGCKFIPFGSGRRGCPGENLAIHVIGL 460

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEW+K     IDM EG G  ++P+VQPL AKC PR  +V L+S
Sbjct: 461 ALGSLLQCFEWDKPNREIIDMSEGTGFTLSPKVQPLLAKCSPRLHMVKLLS 511


>ref|XP_009796227.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X2 [Nicotiana
           sylvestris]
 ref|XP_016503271.1| PREDICTED: isoflavone 2'-hydroxylase-like isoform X2 [Nicotiana
           tabacum]
          Length = 508

 Score =  281 bits (720), Expect = 5e-89
 Identities = 141/224 (62%), Positives = 171/224 (76%), Gaps = 3/224 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIRNL+LVL+Q  S TS+ TLEWAFS LL N +IL KA++E
Sbjct: 281 IMEVLLDLQKTDPEYYTDETIRNLMLVLLQAGSDTSAVTLEWAFSHLLDNLEILKKAQAE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++ Y+  IINETLRMHPAAPLL PHLSS+EC V  + VPRGT+
Sbjct: 341 IDNHVGQDRLIDESDLAQLHYIRCIINETLRMHPAAPLLVPHLSSEECNVASYRVPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W DPEKF P+RF G EG K   K         GCPGENLA H++GL
Sbjct: 401 LLVNAWGIHHDPKVWEDPEKFYPDRFIGFEGIKEGCKFIPFGSGRRGCPGENLAFHVIGL 460

Query: 199 ALGSLIQCFEWEKV--GEIDMREGKGI-ITPRVQPLRAKCIPRP 77
           ALGSL+QCFEWEK   G IDM EG G  ++P+VQPL AKC PRP
Sbjct: 461 ALGSLLQCFEWEKPNRGSIDMSEGTGFTLSPKVQPLLAKCSPRP 504


>ref|XP_004238115.2| PREDICTED: cytochrome P450 81D1-like [Solanum lycopersicum]
          Length = 755

 Score =  288 bits (736), Expect = 7e-89
 Identities = 141/231 (61%), Positives = 178/231 (77%), Gaps = 3/231 (1%)
 Frame = -2

Query: 739 LIEALFDLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSE 560
           ++E L DLQ+ +PEYYTDETIR+L+LVL+Q  S TS+ TLEWAFS LL NP+IL  A++E
Sbjct: 281 IMEVLLDLQKTDPEYYTDETIRSLMLVLLQAGSDTSAVTLEWAFSHLLDNPEILKTAQTE 340

Query: 559 IDSLVGEHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTM 380
           ID+ VG+ RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+EC V G+ VPRGT+
Sbjct: 341 IDNKVGQDRLIDESDLAQLPYIRCIINETLRMHPAAPLLVPHLSSEECKVAGYRVPRGTV 400

Query: 379 LLVNAWDIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGL 200
           LLVNAW I ++P+ W +P+KF P+RF G EG K   K         GCPGENLA+H++GL
Sbjct: 401 LLVNAWGIHHDPKVWKEPQKFNPDRFLGFEGVKEGCKFIPFGSGRRGCPGENLAIHVIGL 460

Query: 199 ALGSLIQCFEWEKVGE--IDMREGKGI-ITPRVQPLRAKCIPRPFVVNLVS 56
           ALGSL+QCFEW+K     IDM EG G  ++P+VQPL AKC PRP +V L+S
Sbjct: 461 ALGSLLQCFEWDKPNREIIDMSEGTGFTLSPKVQPLLAKCSPRPHMVKLLS 511



 Score =  271 bits (693), Expect = 2e-82
 Identities = 134/216 (62%), Positives = 165/216 (76%), Gaps = 3/216 (1%)
 Frame = -2

Query: 721  DLQRAEPEYYTDETIRNLLLVLVQGASHTSSTTLEWAFSLLLQNPDILTKARSEIDSLVG 542
            DLQ+ +P+YYTD+ IRNLLL+L+Q  S TS+ TLEWAFS LL NP+IL  A++EID+ VG
Sbjct: 540  DLQKTDPKYYTDKMIRNLLLILLQAGSDTSAITLEWAFSRLLDNPEILKTAQTEIDNQVG 599

Query: 541  EHRLITESDVSKIPYLLYIINETLRMHPAAPLLTPHLSSDECIVGGFCVPRGTMLLVNAW 362
            + RLI ESD++++PY+  IINETLRMHPAAPLL PHLSS+EC V G+ VPRGT+LLVNAW
Sbjct: 600  QDRLIDESDLAQLPYIRCIINETLRMHPAAPLLLPHLSSEECKVAGYRVPRGTILLVNAW 659

Query: 361  DIQNNPQYWNDPEKFKPERFEGLEGGKNEFKXXXXXXXXXGCPGENLAMHMVGLALGSLI 182
             I ++P+ W DPEKF P+RF G E  K   K         GCPGENLA+H++GLALGSL+
Sbjct: 660  GIHHDPKVWEDPEKFNPDRFIGFESVKEGCKFIPFGSGRRGCPGENLAIHVIGLALGSLL 719

Query: 181  QCFEWEKVGE--IDMREGKGI-ITPRVQPLRAKCIP 83
            QCFEW+K     IDM EG G  I+P+VQPL AKC P
Sbjct: 720  QCFEWDKPNSETIDMSEGTGFTISPKVQPLLAKCSP 755