BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00023464
         (846 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum...   399   e-130
gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branchin...   391   e-127
ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea...   373   e-120
ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea...   373   e-120
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythra...   358   e-115
ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Eryt...   358   e-114
gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]        356   e-114
gb|AOQ26242.1| ISA2 [Actinidia deliciosa]                             352   e-112
ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Sola...   349   e-111
ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   348   e-110
gb|PON65768.1| Glycoside hydrolase [Parasponia andersonii]            348   e-110
gb|PHT55867.1| Isoamylase 2, chloroplastic [Capsicum baccatum]        347   e-110
gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]        347   e-110
ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like ...   347   e-110
gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]          346   e-110
ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like ...   346   e-110
gb|PON52856.1| Glycoside hydrolase [Trema orientalis]                 346   e-109
emb|CDP13520.1| unnamed protein product [Coffea canephora]            346   e-109
ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   345   e-109
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico...   345   e-109

>ref|XP_011092269.1| isoamylase 2, chloroplastic [Sesamum indicum]
 ref|XP_011092270.1| isoamylase 2, chloroplastic [Sesamum indicum]
          Length = 857

 Score =  399 bits (1025), Expect = e-130
 Identities = 196/245 (80%), Positives = 212/245 (86%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFS NFITRNVGLTLVDLVSFS S    + SWNCGEEGPTNKT+VLE RLKQIRNFLFI
Sbjct: 613  PAFSINFITRNVGLTLVDLVSFSSSTFGSELSWNCGEEGPTNKTLVLERRLKQIRNFLFI 672

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQSTGGS  Y DR+PLDWN L SGFG QV +FISFLS L+ RRS
Sbjct: 673  LFISLGVPVLNMGDECGQSTGGSQEYADRRPLDWNALRSGFGTQVTQFISFLSLLRRRRS 732

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            D+LQ RSFLKE+NIEW GT+++PP WDD SCKFLAMTLKADA  SQ TSGS NAGGDLF+
Sbjct: 733  DILQRRSFLKEDNIEWHGTDRTPPMWDDPSCKFLAMTLKADALPSQVTSGSPNAGGDLFV 792

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS +H +KV LP LAV +TWV LVDTALPFPGFFTVDGVP++AGLATYEMKSHSCILF
Sbjct: 793  AFNSANHLQKVALPQLAVGSTWVRLVDTALPFPGFFTVDGVPLDAGLATYEMKSHSCILF 852

Query: 721  EAWSL 735
            EA  L
Sbjct: 853  EARKL 857


>gb|PIM97765.1| 1,4-alpha-glucan branching enzyme/starch branching enzyme II
            [Handroanthus impetiginosus]
          Length = 853

 Score =  391 bits (1005), Expect = e-127
 Identities = 198/245 (80%), Positives = 211/245 (86%), Gaps = 1/245 (0%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNFITRNVGLTLVDLVSFS SDLE + SWNCGEEGPTNK+VVLE RLKQIRNFLFI
Sbjct: 611  PAFSFNFITRNVGLTLVDLVSFSNSDLESELSWNCGEEGPTNKSVVLERRLKQIRNFLFI 670

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGS AY DRK LDWN L SGFGIQV +FISFLSSL+IRRS
Sbjct: 671  LFISLGVPVLNMGDECGQSSGGSTAYADRKLLDWNALKSGFGIQVTQFISFLSSLRIRRS 730

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ RSFLKE+NIEW GT+QSPP WDD SCKFLAMTLKA+A    S +     GGDLFI
Sbjct: 731  DLLQKRSFLKEDNIEWHGTQQSPPTWDDPSCKFLAMTLKAEAPPLSSPN---VIGGDLFI 787

Query: 541  AFNSGDHSEKVTLPPLA-VDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCIL 717
            AFNS DHS KV +PPLA VD TWV LVDTALP PGFF+ DGVP++AGL TYEMKSHSCIL
Sbjct: 788  AFNSADHSAKVAVPPLASVDATWVRLVDTALPLPGFFSKDGVPLDAGLVTYEMKSHSCIL 847

Query: 718  FEAWS 732
            FEA S
Sbjct: 848  FEARS 852


>ref|XP_022895653.1| isoamylase 2, chloroplastic isoform X2 [Olea europaea var.
            sylvestris]
          Length = 829

 Score =  373 bits (957), Expect = e-120
 Identities = 180/245 (73%), Positives = 201/245 (82%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNFI+RN GL LVDLV FS S+L  + SWNCGEEGPTNK  VLE RLKQIRN+LFI
Sbjct: 585  PAFSFNFISRNAGLPLVDLVCFSSSELASELSWNCGEEGPTNKNAVLERRLKQIRNYLFI 644

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LF SLGVPVLNMGDECGQS+GGSPA+ DRKPLDWN L SGF IQ  KFI+FLSSLK+RRS
Sbjct: 645  LFFSLGVPVLNMGDECGQSSGGSPAHADRKPLDWNALKSGFAIQTTKFITFLSSLKMRRS 704

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQNR+F KEENIEW G++ SPP+WD+ SC+FLAMTLKAD++ SQ TSG     GDLFI
Sbjct: 705  DLLQNRNFWKEENIEWHGSDGSPPRWDEPSCRFLAMTLKADSKESQPTSGLHEMIGDLFI 764

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE + LPPL  D  W  LVDT+LPFPGFFT DGVP++ G  TYEMKSHSC+LF
Sbjct: 765  AFNSAGRSENIVLPPLTEDIAWFRLVDTSLPFPGFFTTDGVPLKDGSVTYEMKSHSCVLF 824

Query: 721  EAWSL 735
            EA SL
Sbjct: 825  EARSL 829


>ref|XP_022895652.1| isoamylase 2, chloroplastic isoform X1 [Olea europaea var.
            sylvestris]
          Length = 849

 Score =  373 bits (957), Expect = e-120
 Identities = 180/245 (73%), Positives = 201/245 (82%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNFI+RN GL LVDLV FS S+L  + SWNCGEEGPTNK  VLE RLKQIRN+LFI
Sbjct: 605  PAFSFNFISRNAGLPLVDLVCFSSSELASELSWNCGEEGPTNKNAVLERRLKQIRNYLFI 664

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LF SLGVPVLNMGDECGQS+GGSPA+ DRKPLDWN L SGF IQ  KFI+FLSSLK+RRS
Sbjct: 665  LFFSLGVPVLNMGDECGQSSGGSPAHADRKPLDWNALKSGFAIQTTKFITFLSSLKMRRS 724

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQNR+F KEENIEW G++ SPP+WD+ SC+FLAMTLKAD++ SQ TSG     GDLFI
Sbjct: 725  DLLQNRNFWKEENIEWHGSDGSPPRWDEPSCRFLAMTLKADSKESQPTSGLHEMIGDLFI 784

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE + LPPL  D  W  LVDT+LPFPGFFT DGVP++ G  TYEMKSHSC+LF
Sbjct: 785  AFNSAGRSENIVLPPLTEDIAWFRLVDTSLPFPGFFTTDGVPLKDGSVTYEMKSHSCVLF 844

Query: 721  EAWSL 735
            EA SL
Sbjct: 845  EARSL 849


>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythranthe guttata]
          Length = 747

 Score =  358 bits (918), Expect = e-115
 Identities = 179/245 (73%), Positives = 198/245 (80%), Gaps = 1/245 (0%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNF+TRNVG TLVDLVS++  +     SWNCGEEGPTNK +VLETRLKQIRNF+F+
Sbjct: 516  PAFSFNFVTRNVGPTLVDLVSYNSIE----SSWNCGEEGPTNKILVLETRLKQIRNFIFV 571

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LF+SLGVPVLNMGDECGQST GSP Y+DR   DW+ L SGFG QV +FISFLSSL+IRR+
Sbjct: 572  LFVSLGVPVLNMGDECGQSTRGSPEYSDRISFDWSALKSGFGTQVTEFISFLSSLRIRRN 631

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ RSFLKE+NIEW G E  PP WDD SCKFLAMTLKAD  LS           DLF+
Sbjct: 632  DLLQKRSFLKEDNIEWHGAEGGPPMWDDPSCKFLAMTLKADPPLS-----------DLFV 680

Query: 541  AFNSGDHSEKVTLPPL-AVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCIL 717
            AFN GD SEKV LPPL AVDT WVCLVDT+LPFPGFFT +GVP+E GL TYEMKSHSC+L
Sbjct: 681  AFNGGDCSEKVALPPLAAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLETYEMKSHSCVL 740

Query: 718  FEAWS 732
            FEA S
Sbjct: 741  FEARS 745


>ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842326.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842327.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
 ref|XP_012842328.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttata]
          Length = 840

 Score =  358 bits (918), Expect = e-114
 Identities = 179/245 (73%), Positives = 198/245 (80%), Gaps = 1/245 (0%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNF+TRNVG TLVDLVS++  +     SWNCGEEGPTNK +VLETRLKQIRNF+F+
Sbjct: 609  PAFSFNFVTRNVGPTLVDLVSYNSIE----SSWNCGEEGPTNKILVLETRLKQIRNFIFV 664

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LF+SLGVPVLNMGDECGQST GSP Y+DR   DW+ L SGFG QV +FISFLSSL+IRR+
Sbjct: 665  LFVSLGVPVLNMGDECGQSTRGSPEYSDRISFDWSALKSGFGTQVTEFISFLSSLRIRRN 724

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ RSFLKE+NIEW G E  PP WDD SCKFLAMTLKAD  LS           DLF+
Sbjct: 725  DLLQKRSFLKEDNIEWHGAEGGPPMWDDPSCKFLAMTLKADPPLS-----------DLFV 773

Query: 541  AFNSGDHSEKVTLPPL-AVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCIL 717
            AFN GD SEKV LPPL AVDT WVCLVDT+LPFPGFFT +GVP+E GL TYEMKSHSC+L
Sbjct: 774  AFNGGDCSEKVALPPLAAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLETYEMKSHSCVL 833

Query: 718  FEAWS 732
            FEA S
Sbjct: 834  FEARS 838


>gb|KZV17747.1| isoamylase isoform 2 [Dorcoceras hygrometricum]
          Length = 844

 Score =  356 bits (914), Expect = e-114
 Identities = 173/245 (70%), Positives = 197/245 (80%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+ITRNVGLTL+DLVSF   ++  Q SWNCGEEGPTNK  VLE RLKQIRNFLF+
Sbjct: 600  PAFSFNYITRNVGLTLIDLVSFGSEEIASQLSWNCGEEGPTNKISVLERRLKQIRNFLFL 659

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LF+SLGVPVLNMGDE GQS  GS AYTDR PLDWN L SGF IQ+ +FISFL SLK+RR 
Sbjct: 660  LFLSLGVPVLNMGDEFGQSCAGSLAYTDRTPLDWNSLRSGFSIQITQFISFLCSLKMRRG 719

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            D  Q +SFL+E+ IEW GT+ SPPKWDD S KF+A+TLK   +LSQSTS      GDLF+
Sbjct: 720  DFFQRKSFLEEDKIEWHGTDLSPPKWDDPSTKFVAVTLKVGNKLSQSTSAFPEPSGDLFV 779

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS D SE+V LPPL  +T WV LVDTAL FPGFF+VDGVP+E GL TYEM+SHSC+LF
Sbjct: 780  AFNSDDQSEEVALPPLPDNTAWVRLVDTALTFPGFFSVDGVPLEDGLTTYEMQSHSCVLF 839

Query: 721  EAWSL 735
            EA +L
Sbjct: 840  EARNL 844


>gb|AOQ26242.1| ISA2 [Actinidia deliciosa]
          Length = 866

 Score =  352 bits (904), Expect = e-112
 Identities = 172/248 (69%), Positives = 199/248 (80%), Gaps = 3/248 (1%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            P FSFN+ITRN GL LVDLVSFS S+L  + SWNCGEEGPTN T VLE RLKQIRN+LFI
Sbjct: 617  PGFSFNYITRNSGLPLVDLVSFSSSNLASELSWNCGEEGPTNNTAVLERRLKQIRNYLFI 676

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LF+S GVPVLNMGDECGQS+GGSPAY DRKP DWN L +GFGIQ  +FISFLSSL++RRS
Sbjct: 677  LFVSFGVPVLNMGDECGQSSGGSPAYGDRKPYDWNALRTGFGIQTTQFISFLSSLRVRRS 736

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ RSFLKEENI+W G++QS P WDD S +FLAMTLK + + ++S+S   +  GDLF+
Sbjct: 737  DLLQKRSFLKEENIDWYGSDQSLPTWDDRSSRFLAMTLKVEREENESSSEFSSLNGDLFV 796

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPV---EAGLATYEMKSHSC 711
            AFNS D SE+V LPP   +  W+ LVDTA+PFPGFF+ DG PV    AGL TYEMKSHSC
Sbjct: 797  AFNSADQSERVILPPPPPEMVWLRLVDTAVPFPGFFSRDGEPVLEQMAGLVTYEMKSHSC 856

Query: 712  ILFEAWSL 735
            +LFEA  L
Sbjct: 857  VLFEAKKL 864


>ref|XP_015087879.1| PREDICTED: isoamylase 2, chloroplastic [Solanum pennellii]
          Length = 866

 Score =  349 bits (895), Expect = e-111
 Identities = 170/245 (69%), Positives = 195/245 (79%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I RN GLTLVDLVSFS S++  + SWNCG+EG T  ++VLE RLKQ+RNFLFI
Sbjct: 617  PAFSFNYIARNSGLTLVDLVSFSNSEVASELSWNCGQEGATTNSIVLERRLKQVRNFLFI 676

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSPAY  RK LDWN L +GFG Q+A+FISFLS+L++RRS
Sbjct: 677  LFISLGVPVLNMGDECGQSSGGSPAYDARKSLDWNTLKTGFGTQIAQFISFLSNLRMRRS 736

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ R+FLKEENI+W G++QSPPKWDD S KFLAMTLKADA++S +    +  GGDLF+
Sbjct: 737  DLLQKRNFLKEENIQWHGSDQSPPKWDDPSSKFLAMTLKADAEVSHTLLSDI--GGDLFV 794

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFN    SE V LPP   D  W  LVDTALPFPGFF   G PVE  L  YEMKSHSC+LF
Sbjct: 795  AFNGAGDSESVILPPPPTDMVWYRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCVLF 854

Query: 721  EAWSL 735
            EA  L
Sbjct: 855  EAQRL 859


>ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
 ref|XP_009761455.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
          Length = 863

 Score =  348 bits (893), Expect = e-110
 Identities = 173/245 (70%), Positives = 196/245 (80%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEEGPT   +VLETRLKQ+RNFLFI
Sbjct: 618  PAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRLKQVRNFLFI 677

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSP +  RK  DW+ L +GFGIQ  +FISFL+SL++RRS
Sbjct: 678  LFISLGVPVLNMGDECGQSSGGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRS 737

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ R+FLKEENI+W G++QSPPKWDD S +FLAM+LKADA+ SQ TS S N GGDLF+
Sbjct: 738  DLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMSLKADAEGSQ-TSVSDN-GGDLFV 795

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFPGFF   G PVE GL  YEMK HSC+LF
Sbjct: 796  AFNSAGESESVILPPPPTDVVWHRLVDTALPFPGFFAEKGTPVEDGLVAYEMKPHSCLLF 855

Query: 721  EAWSL 735
            EA  L
Sbjct: 856  EAQKL 860


>gb|PON65768.1| Glycoside hydrolase [Parasponia andersonii]
          Length = 880

 Score =  348 bits (894), Expect = e-110
 Identities = 172/247 (69%), Positives = 198/247 (80%), Gaps = 5/247 (2%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNF+TRN GL LVDLVSFSG +L  + SWNCGEEGPTNKT+VLE RLKQIRNFLFI
Sbjct: 631  PAFSFNFVTRNSGLPLVDLVSFSGEELASELSWNCGEEGPTNKTLVLERRLKQIRNFLFI 690

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            L++SLGVPVLNMGDECGQ+TGGSPAY DRK  DWN L +GFGIQ+ +FISFLSSL+ RRS
Sbjct: 691  LYVSLGVPVLNMGDECGQTTGGSPAYNDRKSFDWNALKTGFGIQITQFISFLSSLRRRRS 750

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQL--SQSTSGSLNAGGDL 534
            DLLQ R FLKEENI+W G++QSPP W++ + KFLAM+L+AD     +Q TS S ++ GDL
Sbjct: 751  DLLQKRKFLKEENIDWHGSDQSPPNWEEPTSKFLAMSLRADEDSVENQMTSESSSSKGDL 810

Query: 535  FIAFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPV---EAGLATYEMKSH 705
            FIAFN+ DH E V LPP A  T W  LVDTALPFPGFF+ DG PV   E+GL  YEMK  
Sbjct: 811  FIAFNAADHPESVILPPPAEGTVWHRLVDTALPFPGFFSTDGEPVVKQESGLFAYEMKPL 870

Query: 706  SCILFEA 726
            SC +FEA
Sbjct: 871  SCTMFEA 877


>gb|PHT55867.1| Isoamylase 2, chloroplastic [Capsicum baccatum]
          Length = 860

 Score =  347 bits (891), Expect = e-110
 Identities = 172/245 (70%), Positives = 193/245 (78%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLFI
Sbjct: 615  PAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFI 674

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSPAY  RK  DWN L +GFGIQ  +FISFLS+L++RRS
Sbjct: 675  LFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFISFLSNLRMRRS 734

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ  +FLKEENI W G +QSPPKWDD S KFLAMTLKADA++SQ+    +  GGDLF+
Sbjct: 735  DLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSDI--GGDLFV 792

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFPGFF   G PVE  L  YEMKSHSC+LF
Sbjct: 793  AFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLF 852

Query: 721  EAWSL 735
            EA  L
Sbjct: 853  EAQRL 857


>gb|PHU26272.1| Isoamylase 2, chloroplastic [Capsicum chinense]
          Length = 862

 Score =  347 bits (891), Expect = e-110
 Identities = 172/245 (70%), Positives = 193/245 (78%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLFI
Sbjct: 617  PAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFI 676

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSPAY  RK  DWN L +GFGIQ  +FISFLS+L++RRS
Sbjct: 677  LFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFISFLSNLRMRRS 736

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ  +FLKEENI W G +QSPPKWDD S KFLAMTLKADA++SQ+    +  GGDLF+
Sbjct: 737  DLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSEI--GGDLFV 794

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFPGFF   G PVE  L  YEMKSHSC+LF
Sbjct: 795  AFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLF 854

Query: 721  EAWSL 735
            EA  L
Sbjct: 855  EAHRL 859


>ref|XP_016475506.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
 ref|XP_016475508.1| PREDICTED: isoamylase 2, chloroplastic-like [Nicotiana tabacum]
          Length = 863

 Score =  347 bits (890), Expect = e-110
 Identities = 172/245 (70%), Positives = 196/245 (80%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEEGPT   +VLETR+KQ+RNFLFI
Sbjct: 618  PAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRVKQVRNFLFI 677

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSP +  RK  DW+ L +GFGIQ  +FISFL+SL++RRS
Sbjct: 678  LFISLGVPVLNMGDECGQSSGGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRS 737

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ R+FLKEENI+W G++QSPPKWDD S +FLAM+LKADA+ SQ TS S N GGDLF+
Sbjct: 738  DLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMSLKADAEGSQ-TSVSDN-GGDLFV 795

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFPGFF   G PVE GL  YEMK HSC+LF
Sbjct: 796  AFNSAGESESVILPPPPTDVVWHRLVDTALPFPGFFAEKGTPVEDGLVAYEMKPHSCLLF 855

Query: 721  EAWSL 735
            EA  L
Sbjct: 856  EAQKL 860


>gb|PHT89964.1| Isoamylase 2, chloroplastic [Capsicum annuum]
          Length = 862

 Score =  346 bits (888), Expect = e-110
 Identities = 171/245 (69%), Positives = 193/245 (78%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLFI
Sbjct: 617  PAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFI 676

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSPAY  RK  DWN L +GFGIQ  +FI+FLS+L++RRS
Sbjct: 677  LFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFIAFLSNLRMRRS 736

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ  +FLKEENI W G +QSPPKWDD S KFLAMTLKADA++SQ+    +  GGDLF+
Sbjct: 737  DLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSDI--GGDLFV 794

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFPGFF   G PVE  L  YEMKSHSC+LF
Sbjct: 795  AFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLF 854

Query: 721  EAWSL 735
            EA  L
Sbjct: 855  EAQRL 859


>ref|XP_016559778.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559779.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
 ref|XP_016559780.1| PREDICTED: isoamylase 2, chloroplastic-like [Capsicum annuum]
          Length = 862

 Score =  346 bits (888), Expect = e-110
 Identities = 171/245 (69%), Positives = 193/245 (78%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I+RN GLTLVDLVSFS S++  + SWNCGEEG T  T+VLE RLKQ+RNFLFI
Sbjct: 617  PAFSFNYISRNFGLTLVDLVSFSSSEVASELSWNCGEEGATTNTIVLERRLKQVRNFLFI 676

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSPAY  RK  DWN L +GFGIQ  +FI+FLS+L++RRS
Sbjct: 677  LFISLGVPVLNMGDECGQSSGGSPAYEARKSFDWNTLKTGFGIQTTQFIAFLSNLRMRRS 736

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ  +FLKEENI W G +QSPPKWDD S KFLAMTLKADA++SQ+    +  GGDLF+
Sbjct: 737  DLLQKSTFLKEENIRWHGIDQSPPKWDDPSSKFLAMTLKADAEVSQTPVSDI--GGDLFV 794

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFPGFF   G PVE  L  YEMKSHSC+LF
Sbjct: 795  AFNSAGDSECVILPPPPTDMVWHRLVDTALPFPGFFDEKGTPVEDELVAYEMKSHSCLLF 854

Query: 721  EAWSL 735
            EA  L
Sbjct: 855  EAQRL 859


>gb|PON52856.1| Glycoside hydrolase [Trema orientalis]
          Length = 880

 Score =  346 bits (888), Expect = e-109
 Identities = 172/247 (69%), Positives = 197/247 (79%), Gaps = 5/247 (2%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNF+TRN GL LVDLVSFSG +L  + SWNCGEEGPTNKT+VLE RLKQIRNFLFI
Sbjct: 631  PAFSFNFVTRNSGLPLVDLVSFSGEELASELSWNCGEEGPTNKTLVLERRLKQIRNFLFI 690

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            L++SLGVPVLNMGDECGQ+TGGSPAY DR   DWN L +GFGIQ+ +FISFLSSL+ RRS
Sbjct: 691  LYVSLGVPVLNMGDECGQTTGGSPAYNDRISFDWNALKTGFGIQITQFISFLSSLRRRRS 750

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQL--SQSTSGSLNAGGDL 534
            DLLQ R FLKEENI+W G++QSPP W++ + KFLAM L+AD     +Q TS S ++ GDL
Sbjct: 751  DLLQKRKFLKEENIDWHGSDQSPPNWEEPTSKFLAMRLRADEDTVENQITSESSSSKGDL 810

Query: 535  FIAFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPV---EAGLATYEMKSH 705
            +IAFN+ DH E V LPP A  T W  LVDTALPFPGFF+ DG PV   E+GL  YEMKS 
Sbjct: 811  YIAFNAADHPESVILPPPAEGTAWHRLVDTALPFPGFFSTDGEPVVKQESGLFAYEMKSL 870

Query: 706  SCILFEA 726
            SC LFEA
Sbjct: 871  SCTLFEA 877


>emb|CDP13520.1| unnamed protein product [Coffea canephora]
          Length = 871

 Score =  346 bits (887), Expect = e-109
 Identities = 172/242 (71%), Positives = 193/242 (79%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFNFI RN GL+LVDLVSFS S L  + SWNCGEEG TNK  VLE RLKQIRNFLFI
Sbjct: 626  PAFSFNFIARNFGLSLVDLVSFSSSKLAKEFSWNCGEEGATNKNDVLERRLKQIRNFLFI 685

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GGSPAY DR   DWN L+SGF IQ  +FISFL+SL+IRRS
Sbjct: 686  LFISLGVPVLNMGDECGQSSGGSPAYGDRNSFDWNALSSGFSIQTVQFISFLTSLRIRRS 745

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ R+FL+EE+IEW G+ Q+PP+WDD + +FLAMTLKA ++  +S S   NA GDLF 
Sbjct: 746  DLLQKRNFLREESIEWHGSNQAPPRWDDAASRFLAMTLKASSEDIESNSVP-NACGDLFA 804

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFN  D SE +TLPP   D  W  LVDTALPFPGFFT +G  +E GLATYEMKSHSC LF
Sbjct: 805  AFNGADLSESITLPPPPADMVWFRLVDTALPFPGFFTANGACIEDGLATYEMKSHSCALF 864

Query: 721  EA 726
            EA
Sbjct: 865  EA 866


>ref|XP_019231687.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana attenuata]
 gb|OIT28559.1| isoamylase 2, chloroplastic [Nicotiana attenuata]
          Length = 863

 Score =  345 bits (885), Expect = e-109
 Identities = 172/245 (70%), Positives = 194/245 (79%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEEGP    +VLETRLKQ+RNFLFI
Sbjct: 618  PAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEEGPMTNNIVLETRLKQVRNFLFI 677

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+GG P +  RK  DW+ L +GFGIQ  +FISFL+SL++RRS
Sbjct: 678  LFISLGVPVLNMGDECGQSSGGFPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRS 737

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ R+FLKEENI+W G++QSPPKWDD S +FLAMTLKADA+ SQ TS S N GGDLF+
Sbjct: 738  DLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMTLKADAEDSQ-TSVSDN-GGDLFV 795

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDT LPFPGFF   G PVE GL  YEMKSHSC+LF
Sbjct: 796  AFNSAGESESVILPPPPTDMVWHRLVDTGLPFPGFFAEKGTPVEDGLVAYEMKSHSCLLF 855

Query: 721  EAWSL 735
            EA  L
Sbjct: 856  EAQKL 860


>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
 ref|XP_018625653.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
          Length = 863

 Score =  345 bits (885), Expect = e-109
 Identities = 173/245 (70%), Positives = 195/245 (79%)
 Frame = +1

Query: 1    PAFSFNFITRNVGLTLVDLVSFSGSDLEPQCSWNCGEEGPTNKTVVLETRLKQIRNFLFI 180
            PAFSFN+I RN GLTLVDLVSFS S++  + SWNCGEEGPT   +VLETRLKQ+RNFLFI
Sbjct: 618  PAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEEGPTTNNIVLETRLKQVRNFLFI 677

Query: 181  LFISLGVPVLNMGDECGQSTGGSPAYTDRKPLDWNGLNSGFGIQVAKFISFLSSLKIRRS 360
            LFISLGVPVLNMGDECGQS+ GSP +  RK  DW+ L +GFGIQ  +FISFL+SL++RRS
Sbjct: 678  LFISLGVPVLNMGDECGQSSRGSPVHGARKSFDWSTLKTGFGIQTTQFISFLTSLRMRRS 737

Query: 361  DLLQNRSFLKEENIEWRGTEQSPPKWDDESCKFLAMTLKADAQLSQSTSGSLNAGGDLFI 540
            DLLQ R+FLKEENI+W G++QSPPKWDD S +FLAMTLKADA+ SQ TS S N GGDLF+
Sbjct: 738  DLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMTLKADAEDSQ-TSVSDN-GGDLFV 795

Query: 541  AFNSGDHSEKVTLPPLAVDTTWVCLVDTALPFPGFFTVDGVPVEAGLATYEMKSHSCILF 720
            AFNS   SE V LPP   D  W  LVDTALPFP FF   G PVE GL  YEMKSHSC+LF
Sbjct: 796  AFNSAGESESVILPPPPTDMVWHRLVDTALPFPEFFAEKGTPVEDGLVAYEMKSHSCLLF 855

Query: 721  EAWSL 735
            EA  L
Sbjct: 856  EAQKL 860