BLASTX nr result
ID: Rehmannia32_contig00023391
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Rehmannia32_contig00023391
(409 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|PIM99977.1| Histone acetyltransferase [Handroanthus impetigin... 167 3e-45
ref|XP_011089969.1| uncharacterized protein LOC105170763 [Sesamu... 164 3e-44
ref|XP_020552448.1| uncharacterized protein LOC105170882 [Sesamu... 162 6e-44
gb|EYU36123.1| hypothetical protein MIMGU_mgv1a0009921mg, partia... 163 7e-44
ref|XP_012838583.1| PREDICTED: uncharacterized protein LOC105959... 163 7e-44
gb|KZV30763.1| hypothetical protein F511_40713 [Dorcoceras hygro... 142 2e-36
gb|EPS71321.1| hypothetical protein M569_03436, partial [Genlise... 129 4e-32
ref|XP_010651762.1| PREDICTED: uncharacterized protein LOC100260... 124 2e-30
emb|CBI17122.3| unnamed protein product, partial [Vitis vinifera] 124 2e-30
emb|CAN79441.1| hypothetical protein VITISV_017668 [Vitis vinifera] 124 2e-30
ref|XP_010651761.1| PREDICTED: uncharacterized protein LOC100260... 124 2e-30
gb|AAS79577.1| putative PHD zinc finger protein [Ipomoea trifida... 124 2e-30
emb|CDP07019.1| unnamed protein product [Coffea canephora] 124 4e-30
gb|OWM73735.1| hypothetical protein CDL15_Pgr026839 [Punica gran... 124 4e-30
gb|PKI64566.1| hypothetical protein CRG98_014998 [Punica granatum] 124 4e-30
emb|CBI17123.3| unnamed protein product, partial [Vitis vinifera] 122 1e-29
emb|CAN78676.1| hypothetical protein VITISV_001802 [Vitis vinifera] 122 1e-29
ref|XP_002272497.2| PREDICTED: uncharacterized protein LOC100255... 122 1e-29
dbj|BAF36342.1| hypothetical protein [Ipomoea trifida] 122 2e-29
ref|XP_011657045.1| PREDICTED: uncharacterized protein LOC101214... 121 3e-29
>gb|PIM99977.1| Histone acetyltransferase [Handroanthus impetiginosus]
Length = 901
Score = 167 bits (422), Expect = 3e-45
Identities = 81/113 (71%), Positives = 92/113 (81%), Gaps = 5/113 (4%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
RR+PES L GVI+GTGILCSCD+CKGR+VVTPN FE HA SGNKRPPEYIYLENGKSLRD
Sbjct: 270 RRRPESGLHGVIQGTGILCSCDECKGRKVVTPNQFELHARSGNKRPPEYIYLENGKSLRD 329
Query: 227 VLNACKANRSDSLALVIQNAIGRSNFTTAFCINCKGDIL-----WTLFFCKSC 370
VLNACK N S+SL LVI+NAIGR ++T AFC+NCKG I ++ C SC
Sbjct: 330 VLNACKVNHSESLELVIRNAIGRPSYTNAFCLNCKGLIPEAGAGRSMLLCDSC 382
>ref|XP_011089969.1| uncharacterized protein LOC105170763 [Sesamum indicum]
ref|XP_020552596.1| uncharacterized protein LOC105170763 [Sesamum indicum]
ref|XP_020552597.1| uncharacterized protein LOC105170763 [Sesamum indicum]
Length = 908
Score = 164 bits (415), Expect = 3e-44
Identities = 81/115 (70%), Positives = 94/115 (81%), Gaps = 6/115 (5%)
Frame = +2
Query: 44 GRRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
GRR+PESEL GVI+G GILCSCD+CK R++VTPN FE HA SGNKRPPEYI+L+NGKSLR
Sbjct: 278 GRRRPESELCGVIQGAGILCSCDECKERKIVTPNQFEMHARSGNKRPPEYIFLDNGKSLR 337
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNFTTAFCINCKGDIL------WTLFFCKSC 370
DVLNACKAN S+SL VI NAIGRSN+TTAFCINCK D++ ++ C SC
Sbjct: 338 DVLNACKANLSESLESVILNAIGRSNYTTAFCINCK-DLIPQAGAGRSMLLCDSC 391
>ref|XP_020552448.1| uncharacterized protein LOC105170882 [Sesamum indicum]
Length = 769
Score = 162 bits (411), Expect = 6e-44
Identities = 76/96 (79%), Positives = 85/96 (88%)
Frame = +2
Query: 44 GRRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
GR+ PE EL GVI+G GILCSCD+CK R++VTPN FE HAGSGNKRPPEYI+L+NGKSLR
Sbjct: 180 GRKWPEFELPGVIQGAGILCSCDECKERKIVTPNQFEMHAGSGNKRPPEYIFLDNGKSLR 239
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNFTTAFCINCK 331
DVLNACKAN S+SL VI NAIGRSN+TTAFCINCK
Sbjct: 240 DVLNACKANLSESLESVILNAIGRSNYTTAFCINCK 275
>gb|EYU36123.1| hypothetical protein MIMGU_mgv1a0009921mg, partial [Erythranthe
guttata]
Length = 905
Score = 163 bits (412), Expect = 7e-44
Identities = 80/114 (70%), Positives = 91/114 (79%), Gaps = 5/114 (4%)
Frame = +2
Query: 44 GRRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
GRR+PESELQG I+GTGILCSCD+C G +VVTPN FE HA SGNKRPPEYIYL+NGKSLR
Sbjct: 274 GRRRPESELQGTIQGTGILCSCDECNGSKVVTPNQFELHAHSGNKRPPEYIYLDNGKSLR 333
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNFTTAFCINCKGDIL-----WTLFFCKSC 370
DVLNACK + S+SL VIQNAIGRS + TAFCI+CKG I ++ C SC
Sbjct: 334 DVLNACKVDLSNSLEFVIQNAIGRSVYITAFCISCKGLIPEAGAGRSMLLCNSC 387
>ref|XP_012838583.1| PREDICTED: uncharacterized protein LOC105959098 [Erythranthe
guttata]
Length = 918
Score = 163 bits (412), Expect = 7e-44
Identities = 80/114 (70%), Positives = 91/114 (79%), Gaps = 5/114 (4%)
Frame = +2
Query: 44 GRRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
GRR+PESELQG I+GTGILCSCD+C G +VVTPN FE HA SGNKRPPEYIYL+NGKSLR
Sbjct: 287 GRRRPESELQGTIQGTGILCSCDECNGSKVVTPNQFELHAHSGNKRPPEYIYLDNGKSLR 346
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNFTTAFCINCKGDIL-----WTLFFCKSC 370
DVLNACK + S+SL VIQNAIGRS + TAFCI+CKG I ++ C SC
Sbjct: 347 DVLNACKVDLSNSLEFVIQNAIGRSVYITAFCISCKGLIPEAGAGRSMLLCNSC 400
>gb|KZV30763.1| hypothetical protein F511_40713 [Dorcoceras hygrometricum]
Length = 796
Score = 142 bits (357), Expect = 2e-36
Identities = 70/100 (70%), Positives = 81/100 (81%), Gaps = 2/100 (2%)
Frame = +2
Query: 47 RRQPESE-LQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
R QP SE L+G I+GTGILCSC++CKG +VVTPN FE HAGSGNKRPPEYIYLENGK+LR
Sbjct: 179 REQPGSENLRGFIRGTGILCSCEECKGEKVVTPNQFELHAGSGNKRPPEYIYLENGKNLR 238
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDI 340
DVLNACK SDSL +VI+N IGR++ A C+ CKG I
Sbjct: 239 DVLNACKREHSDSLDVVIRNFIGRADLRPPASCLKCKGSI 278
>gb|EPS71321.1| hypothetical protein M569_03436, partial [Genlisea aurea]
Length = 668
Score = 129 bits (324), Expect = 4e-32
Identities = 69/115 (60%), Positives = 78/115 (67%), Gaps = 7/115 (6%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R P EL+G+I+GTGILC CD C GR+ VTPN FE HA SGNK PPE IYLENGKSLR+
Sbjct: 31 RIPPNFELRGIIQGTGILCFCDVCDGRKAVTPNKFELHAHSGNKSPPECIYLENGKSLRN 90
Query: 227 VLNACKANRSD--SLALVIQNAIGRSNFTTAFCINCKG-----DILWTLFFCKSC 370
VLN CKAN SD S+ VI +AIG SN TTA C CKG D + C +C
Sbjct: 91 VLNLCKANDSDSGSVVQVILDAIGCSNSTTALCHACKGIIPEADADSDMLLCNAC 145
>ref|XP_010651762.1| PREDICTED: uncharacterized protein LOC100260233 isoform X2 [Vitis
vinifera]
Length = 768
Score = 124 bits (312), Expect = 2e-30
Identities = 65/99 (65%), Positives = 73/99 (73%), Gaps = 1/99 (1%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R ES L+GVIKG+GILCSC+ CKG +VVTPNLFE HAGS NKRPPEYIYLENG SLR
Sbjct: 235 RGSGESGLRGVIKGSGILCSCNSCKGTKVVTPNLFELHAGSSNKRPPEYIYLENGTSLRG 294
Query: 227 VLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDI 340
V+NA K DSL I+ AIG S + FC+NCKG I
Sbjct: 295 VMNAWKNAALDSLDEAIRVAIGCSMIKKSTFCLNCKGRI 333
>emb|CBI17122.3| unnamed protein product, partial [Vitis vinifera]
Length = 824
Score = 124 bits (312), Expect = 2e-30
Identities = 65/99 (65%), Positives = 73/99 (73%), Gaps = 1/99 (1%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R ES L+GVIKG+GILCSC+ CKG +VVTPNLFE HAGS NKRPPEYIYLENG SLR
Sbjct: 235 RGSGESGLRGVIKGSGILCSCNSCKGTKVVTPNLFELHAGSSNKRPPEYIYLENGTSLRG 294
Query: 227 VLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDI 340
V+NA K DSL I+ AIG S + FC+NCKG I
Sbjct: 295 VMNAWKNAALDSLDEAIRVAIGCSMIKKSTFCLNCKGRI 333
>emb|CAN79441.1| hypothetical protein VITISV_017668 [Vitis vinifera]
Length = 848
Score = 124 bits (312), Expect = 2e-30
Identities = 65/99 (65%), Positives = 73/99 (73%), Gaps = 1/99 (1%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R ES L+GVIKG+GILCSC+ CKG +VVTPNLFE HAGS NKRPPEYIYLENG SLR
Sbjct: 235 RGSGESGLRGVIKGSGILCSCNSCKGTKVVTPNLFELHAGSSNKRPPEYIYLENGTSLRG 294
Query: 227 VLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDI 340
V+NA K DSL I+ AIG S + FC+NCKG I
Sbjct: 295 VMNAWKNAALDSLDEAIRVAIGCSMIKKSTFCLNCKGRI 333
>ref|XP_010651761.1| PREDICTED: uncharacterized protein LOC100260233 isoform X1 [Vitis
vinifera]
Length = 856
Score = 124 bits (312), Expect = 2e-30
Identities = 65/99 (65%), Positives = 73/99 (73%), Gaps = 1/99 (1%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R ES L+GVIKG+GILCSC+ CKG +VVTPNLFE HAGS NKRPPEYIYLENG SLR
Sbjct: 235 RGSGESGLRGVIKGSGILCSCNSCKGTKVVTPNLFELHAGSSNKRPPEYIYLENGTSLRG 294
Query: 227 VLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDI 340
V+NA K DSL I+ AIG S + FC+NCKG I
Sbjct: 295 VMNAWKNAALDSLDEAIRVAIGCSMIKKSTFCLNCKGRI 333
>gb|AAS79577.1| putative PHD zinc finger protein [Ipomoea trifida]
dbj|BAF36299.1| hypothetical protein [Ipomoea trifida]
Length = 1047
Score = 124 bits (312), Expect = 2e-30
Identities = 64/116 (55%), Positives = 77/116 (66%), Gaps = 6/116 (5%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R +PE LQGVI+G+GILC C C G +VVTPN FE HAGS NKRPPEYIYL+NGK+LRD
Sbjct: 396 RGRPEKGLQGVIQGSGILCFCQNCGGTKVVTPNQFEMHAGSSNKRPPEYIYLQNGKTLRD 455
Query: 227 VLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDILWTLF-----FCKSCCT 376
VL ACK +D+L I+NA G + + FC+NCK + F C SC T
Sbjct: 456 VLVACKDAPADALEAAIRNATGAGDARKSTFCLNCKASLPEASFGRPRLQCDSCMT 511
>emb|CDP07019.1| unnamed protein product [Coffea canephora]
Length = 865
Score = 124 bits (310), Expect = 4e-30
Identities = 58/88 (65%), Positives = 69/88 (78%)
Frame = +2
Query: 68 LQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRDVLNACKA 247
L GVIKG+G+LC C C G +VVTPN FE HAGSGNKRPPEYIYLENGKSLRD+LN CK
Sbjct: 253 LSGVIKGSGVLCYCRDCNGSQVVTPNQFELHAGSGNKRPPEYIYLENGKSLRDILNICKD 312
Query: 248 NRSDSLALVIQNAIGRSNFTTAFCINCK 331
++SL L +Q+AIG++ T FC+ CK
Sbjct: 313 APAESLELAVQSAIGQARPT--FCLGCK 338
>gb|OWM73735.1| hypothetical protein CDL15_Pgr026839 [Punica granatum]
Length = 966
Score = 124 bits (310), Expect = 4e-30
Identities = 62/115 (53%), Positives = 78/115 (67%), Gaps = 6/115 (5%)
Frame = +2
Query: 44 GRRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
G Q + LQGVIKG+GILCSCDKCKG +VV+P +FE HAGS NKRPPEYI+LENG++LR
Sbjct: 298 GLLQGRTGLQGVIKGSGILCSCDKCKGVQVVSPTIFELHAGSSNKRPPEYIFLENGRTLR 357
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNFT-TAFCINCKGDIL-----WTLFFCKSC 370
DV+NA K + +++ +Q +G S+ CINCKG I T CK C
Sbjct: 358 DVMNAVKDSPLETVEEAVQAVLGTSSINKCTICINCKGPITEVEGGKTTLLCKIC 412
>gb|PKI64566.1| hypothetical protein CRG98_014998 [Punica granatum]
Length = 989
Score = 124 bits (310), Expect = 4e-30
Identities = 62/115 (53%), Positives = 78/115 (67%), Gaps = 6/115 (5%)
Frame = +2
Query: 44 GRRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLR 223
G Q + LQGVIKG+GILCSCDKCKG +VV+P +FE HAGS NKRPPEYI+LENG++LR
Sbjct: 298 GLLQGRTGLQGVIKGSGILCSCDKCKGVQVVSPTIFELHAGSSNKRPPEYIFLENGRTLR 357
Query: 224 DVLNACKANRSDSLALVIQNAIGRSNFT-TAFCINCKGDIL-----WTLFFCKSC 370
DV+NA K + +++ +Q +G S+ CINCKG I T CK C
Sbjct: 358 DVMNAVKDSPLETVEEAVQAVLGTSSINKCTICINCKGPITEVEGGKTTLLCKIC 412
>emb|CBI17123.3| unnamed protein product, partial [Vitis vinifera]
Length = 772
Score = 122 bits (307), Expect = 1e-29
Identities = 63/109 (57%), Positives = 72/109 (66%), Gaps = 5/109 (4%)
Frame = +2
Query: 59 ESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRDVLNA 238
ES L GVIKG+GILC CD CKG VVTPN+FE HAGS NKRPPEYIYLENG +LR V+ A
Sbjct: 305 ESGLHGVIKGSGILCYCDTCKGTNVVTPNVFELHAGSSNKRPPEYIYLENGNTLRSVMTA 364
Query: 239 CKANRSDSLALVIQNAIGRSNFTTAFCINCKGDILW-----TLFFCKSC 370
C +L I+ AIG S + FC NCKG I +L C+SC
Sbjct: 365 CSKATLKALDEDIRVAIGSSIKKSTFCFNCKGSISEVGTSDSLVLCESC 413
>emb|CAN78676.1| hypothetical protein VITISV_001802 [Vitis vinifera]
Length = 844
Score = 122 bits (307), Expect = 1e-29
Identities = 63/109 (57%), Positives = 72/109 (66%), Gaps = 5/109 (4%)
Frame = +2
Query: 59 ESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRDVLNA 238
ES L GVIKG+GILC CD CKG VVTPN+FE HAGS NKRPPEYIYLENG +LR V+ A
Sbjct: 243 ESGLHGVIKGSGILCYCDTCKGTNVVTPNVFELHAGSSNKRPPEYIYLENGNTLRSVMTA 302
Query: 239 CKANRSDSLALVIQNAIGRSNFTTAFCINCKGDILW-----TLFFCKSC 370
C +L I+ AIG S + FC NCKG I +L C+SC
Sbjct: 303 CSKATLKALDEDIRVAIGSSIKKSTFCFNCKGSISEVGTSDSLVLCESC 351
>ref|XP_002272497.2| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_010651755.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_010651756.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_010651757.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_010651758.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_010651760.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_019076200.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_019076201.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
ref|XP_019076203.1| PREDICTED: uncharacterized protein LOC100255152 [Vitis vinifera]
Length = 863
Score = 122 bits (307), Expect = 1e-29
Identities = 63/109 (57%), Positives = 72/109 (66%), Gaps = 5/109 (4%)
Frame = +2
Query: 59 ESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRDVLNA 238
ES L GVIKG+GILC CD CKG VVTPN+FE HAGS NKRPPEYIYLENG +LR V+ A
Sbjct: 243 ESGLHGVIKGSGILCYCDTCKGTNVVTPNVFELHAGSSNKRPPEYIYLENGNTLRSVMTA 302
Query: 239 CKANRSDSLALVIQNAIGRSNFTTAFCINCKGDILW-----TLFFCKSC 370
C +L I+ AIG S + FC NCKG I +L C+SC
Sbjct: 303 CSKATLKALDEDIRVAIGSSIKKSTFCFNCKGSISEVGTSDSLVLCESC 351
>dbj|BAF36342.1| hypothetical protein [Ipomoea trifida]
Length = 770
Score = 122 bits (305), Expect = 2e-29
Identities = 63/116 (54%), Positives = 76/116 (65%), Gaps = 6/116 (5%)
Frame = +2
Query: 47 RRQPESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRD 226
R +PE LQGVI+G+GILC C C G +VVTPN FE HAGS NKRPPEYIYL+NGK+LRD
Sbjct: 391 RGRPEKGLQGVIQGSGILCFCQNCGGTKVVTPNQFEMHAGSSNKRPPEYIYLQNGKTLRD 450
Query: 227 VLNACKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDILWTLF-----FCKSCCT 376
VL ACK +D+L I+NA G + + C+NCK + F C SC T
Sbjct: 451 VLVACKDAPADALEAAIRNATGAGDARKSTVCLNCKASLPEASFGRPRLQCDSCMT 506
>ref|XP_011657045.1| PREDICTED: uncharacterized protein LOC101214170 isoform X2 [Cucumis
sativus]
Length = 910
Score = 121 bits (304), Expect = 3e-29
Identities = 62/111 (55%), Positives = 76/111 (68%), Gaps = 3/111 (2%)
Frame = +2
Query: 59 ESELQGVIKGTGILCSCDKCKGREVVTPNLFETHAGSGNKRPPEYIYLENGKSLRDVLNA 238
E+ L GVI G+GI+C C+ CKG+EVV+P LFE HAGS NKRPPEYIYLE G +LRD++NA
Sbjct: 335 ETGLGGVISGSGIICFCNNCKGKEVVSPTLFELHAGSSNKRPPEYIYLETGNTLRDIMNA 394
Query: 239 CKANRSDSLALVIQNAIGRSNF-TTAFCINCKGDI--LWTLFFCKSCCTCV 382
C+ D IQ+AIGRS TA C+NCKG I T CC+C+
Sbjct: 395 CQNFSFDQTEEFIQSAIGRSLVKRTAICLNCKGRIPESDTGIAMLLCCSCM 445