BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00019323
         (1881 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011086391.1| alpha-amylase 3, chloroplastic [Sesamum indi...   956   0.0  
gb|PIN17014.1| Alpha-amylase [Handroanthus impetiginosus]             875   0.0  
ref|XP_022866855.1| alpha-amylase 3, chloroplastic [Olea europae...   834   0.0  
ref|XP_012855272.1| PREDICTED: alpha-amylase 3, chloroplastic [E...   778   0.0  
emb|CDP00291.1| unnamed protein product [Coffea canephora]            747   0.0  
ref|XP_009599231.1| PREDICTED: alpha-amylase 3, chloroplastic is...   743   0.0  
ref|XP_009599230.1| PREDICTED: alpha-amylase 3, chloroplastic is...   738   0.0  
ref|XP_009758349.1| PREDICTED: alpha-amylase 3, chloroplastic is...   736   0.0  
gb|AAX33233.1| plastid alpha-amylase [Actinidia chinensis]            731   0.0  
ref|XP_009758348.1| PREDICTED: alpha-amylase 3, chloroplastic is...   731   0.0  
gb|AFO84072.1| alpha-amylase [Actinidia chinensis]                    730   0.0  
ref|XP_019256898.1| PREDICTED: alpha-amylase 3, chloroplastic [N...   727   0.0  
ref|XP_019170778.1| PREDICTED: alpha-amylase 3, chloroplastic is...   726   0.0  
ref|XP_023928052.1| alpha-amylase 3, chloroplastic isoform X2 [Q...   717   0.0  
ref|XP_010256483.1| PREDICTED: alpha-amylase 3, chloroplastic-li...   717   0.0  
gb|POE91280.1| alpha-amylase 3, chloroplastic [Quercus suber]         717   0.0  
gb|OMP12226.1| hypothetical protein CCACVL1_00071 [Corchorus cap...   705   0.0  
ref|XP_022776933.1| alpha-amylase 3, chloroplastic-like isoform ...   708   0.0  
ref|XP_016547075.1| PREDICTED: alpha-amylase 3, chloroplastic [C...   712   0.0  
ref|XP_015075683.1| PREDICTED: alpha-amylase 3, chloroplastic [S...   711   0.0  

>ref|XP_011086391.1| alpha-amylase 3, chloroplastic [Sesamum indicum]
          Length = 929

 Score =  956 bits (2470), Expect = 0.0
 Identities = 473/629 (75%), Positives = 524/629 (83%), Gaps = 3/629 (0%)
 Frame = -3

Query: 1879 KMSVVTTDRLFL---HHHTCTTFKPVQKFQFISKKLQPFNPICPRPRTRRANICSRHFYI 1709
            KMSVVTTD  FL   HH   T+FKPV KF+ IS++ QPF+  C +    R N+ +RH   
Sbjct: 20   KMSVVTTDPRFLLLHHHRRATSFKPVSKFKLISQRRQPFHLNCRQTPNSRGNVGTRHSCP 79

Query: 1708 PKALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLP 1529
              ALSSSG++VVETSESS+VTF ETF L+RPEKLEGKITIRLD+G+NEE  KLTVGC+LP
Sbjct: 80   LNALSSSGSSVVETSESSDVTFRETFRLQRPEKLEGKITIRLDHGENEEYGKLTVGCNLP 139

Query: 1528 GKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKI 1349
            GKWVLHWGVNY+GDVGSEWDQPPLDMRP GSIPIKDYAIETP  RSP  SEGE F+EVKI
Sbjct: 140  GKWVLHWGVNYVGDVGSEWDQPPLDMRPPGSIPIKDYAIETPLGRSPALSEGEVFYEVKI 199

Query: 1348 DFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGAL 1169
            DF+T+SSIAAINFVLKDEE G WYQHRGRDFK+PLIDYLQDDGN++GAKK LG+W  GAL
Sbjct: 200  DFSTNSSIAAINFVLKDEEGGNWYQHRGRDFKIPLIDYLQDDGNILGAKKSLGLWP-GAL 258

Query: 1168 GQISNMLHKPEAADSKGDDIRESNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERA 989
            GQIS+++ K   AD KGDDI ESNLQKRPLQ FYEEHSVFKE +TDN++S+S  +CLERA
Sbjct: 259  GQISSVILKSNTADYKGDDIVESNLQKRPLQGFYEEHSVFKEFVTDNAMSISVRYCLERA 318

Query: 988  KNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGS 809
            KNIL IETDLPGDVV+HWGV  DE K+WEIP EPYPPET  FKNKALRTLLQ+KDDG GS
Sbjct: 319  KNILFIETDLPGDVVLHWGVHKDESKSWEIPPEPYPPETTMFKNKALRTLLQQKDDGCGS 378

Query: 808  WGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEF 629
            WG FTLD  FSAFVFVLKLNENTWLNC G+DF+IP T SV TD+ S LTHS+ EE LK+F
Sbjct: 379  WGLFTLDDVFSAFVFVLKLNENTWLNCKGDDFFIPLTRSVVTDKDSGLTHSQDEEQLKDF 438

Query: 628  GSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAA 449
            GS      TS+SN+A  A+ DGIINEIRNLV+DI           EAQESIL EIEKLAA
Sbjct: 439  GSSEKFGDTSDSNQAGPAYADGIINEIRNLVNDISSEKHGKTKSVEAQESILHEIEKLAA 498

Query: 448  EAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMELY 269
            EAYSIFRSSMPT+ DT++SED+DLQ           GYEILCQGFNWESHKSG WYMEL+
Sbjct: 499  EAYSIFRSSMPTFIDTNLSEDEDLQPPVKISSGTGSGYEILCQGFNWESHKSGNWYMELH 558

Query: 268  EKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKV 89
            EKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGN+DQLK LV +LHEVGI+V
Sbjct: 559  EKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNLDQLKVLVKRLHEVGIRV 618

Query: 88   LGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            LGDVVLNHRCAQYKNQNGVWNIFGGRLNW
Sbjct: 619  LGDVVLNHRCAQYKNQNGVWNIFGGRLNW 647


>gb|PIN17014.1| Alpha-amylase [Handroanthus impetiginosus]
          Length = 903

 Score =  875 bits (2261), Expect = 0.0
 Identities = 444/628 (70%), Positives = 498/628 (79%), Gaps = 3/628 (0%)
 Frame = -3

Query: 1876 MSVVTTDRLFLHHHTCTTFKPVQKFQFISKKLQPFNPICPRPRTRRAN--ICSRHFYIPK 1703
            M+ VT   LF H    T+ KP+ KFQ IS K  PF+  C    +   N  I SR+F   K
Sbjct: 1    MATVTMGPLFHHR---TSLKPIPKFQLISTKPPPFHLNCTLTPSSPFNGAIFSRYFKASK 57

Query: 1702 ALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEE-CWKLTVGCSLPG 1526
            A+SS+GA VVET ESSNVTFSE F L+R +K+EGKIT+R D GKN+E CW+LTVGC LPG
Sbjct: 58   AVSSTGAAVVETLESSNVTFSENFKLERAQKVEGKITVRFDCGKNDENCWRLTVGCGLPG 117

Query: 1525 KWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKID 1346
            KW+LHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYA+ETPF+R   S+ GEAF E+ I+
Sbjct: 118  KWILHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYALETPFQRK--SAGGEAFSEIMIE 175

Query: 1345 FNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALG 1166
              + SS+AAINFVLKD ESG WYQHRGRDFKVPLID LQDD NVVG   GL  W  GA+G
Sbjct: 176  IKSKSSVAAINFVLKDAESGNWYQHRGRDFKVPLIDSLQDDDNVVGTINGLSTWP-GAVG 234

Query: 1165 QISNMLHKPEAADSKGDDIRESNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERAK 986
            Q+SN L KPEAA+SKG+DI + NL+ RPLQ FYEEHSVFKEI+  NS+SVS  HC E+AK
Sbjct: 235  QLSNKLLKPEAAESKGEDIGKLNLRSRPLQGFYEEHSVFKEIVAFNSLSVSVRHCQEKAK 294

Query: 985  NILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGSW 806
            N+L+IETDL GDVV+HWGVC DE +NWEIPAEPYPPET  F+NKALRTLLQ+K +G GS 
Sbjct: 295  NVLYIETDLLGDVVVHWGVCKDESRNWEIPAEPYPPETTVFRNKALRTLLQQKTEGDGSC 354

Query: 805  GSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFG 626
            GSFTLD+   AFVFVLKLNENTWLNC GNDFYIPF++SV    HS  THS+ +E  +EF 
Sbjct: 355  GSFTLDEGLFAFVFVLKLNENTWLNCKGNDFYIPFSTSVVPHRHSGPTHSEVKEEHEEFS 414

Query: 625  SPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAAE 446
            SPV     S++N+A SA+TD IINEIRNLVSDI           EAQESILQEIEKLAAE
Sbjct: 415  SPVISTDASKTNQADSAYTDEIINEIRNLVSDISSEKSRKTKSKEAQESILQEIEKLAAE 474

Query: 445  AYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMELYE 266
            AYSIFRSS PT  +TDV E++ LQ           G+EILCQGFNWESHKSGKWYMEL E
Sbjct: 475  AYSIFRSSQPTSPETDVLEEEALQAPLKISSGTGSGFEILCQGFNWESHKSGKWYMELQE 534

Query: 265  KASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKVL 86
            KAS+LSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLK LV +LHEVGI VL
Sbjct: 535  KASKLSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKVLVKRLHEVGIMVL 594

Query: 85   GDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            GDVVLNHRCAQYKNQNGVWNIFGGRLNW
Sbjct: 595  GDVVLNHRCAQYKNQNGVWNIFGGRLNW 622


>ref|XP_022866855.1| alpha-amylase 3, chloroplastic [Olea europaea var. sylvestris]
          Length = 910

 Score =  834 bits (2155), Expect = 0.0
 Identities = 416/591 (70%), Positives = 480/591 (81%), Gaps = 5/591 (0%)
 Frame = -3

Query: 1759 PRPRTRRA-NICSRHFYIPKALS----SSGATVVETSESSNVTFSETFHLKRPEKLEGKI 1595
            PRP    A ++ S HF   KALS    S    VVETSESS+  FSETF LKRP+K+EGKI
Sbjct: 41   PRPIFNDACSLSSHHFKPAKALSLRTSSIDTAVVETSESSDFVFSETFQLKRPQKVEGKI 100

Query: 1594 TIRLDNGKNEECWKLTVGCSLPGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYA 1415
            TIRL++G +++ W+LTVGC LPGKW+LHWGVNYIGDVGSEWDQPP DMRP GSIPIKDYA
Sbjct: 101  TIRLESGNSDDNWRLTVGCCLPGKWILHWGVNYIGDVGSEWDQPPPDMRPPGSIPIKDYA 160

Query: 1414 IETPFERSPTSSEGEAFHEVKIDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDY 1235
            IETP E+S  SSEGE+ +EV IDFNT+SSIAAINFVLKDEE+G WYQHRGRDFKVPLIDY
Sbjct: 161  IETPLEKSLASSEGESIYEVNIDFNTNSSIAAINFVLKDEETGSWYQHRGRDFKVPLIDY 220

Query: 1234 LQDDGNVVGAKKGLGIWQAGALGQISNMLHKPEAADSKGDDIRESNLQKRPLQAFYEEHS 1055
            LQDDGN+VGAKKGLG W  G LGQISNML KPEAA+SK +DI E ++QKR LQ FYEE +
Sbjct: 221  LQDDGNIVGAKKGLGTWP-GPLGQISNMLLKPEAANSK-EDIGELSVQKRGLQGFYEEQA 278

Query: 1054 VFKEIITDNSISVSASHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPE 875
            V KEI+ DNS+SVS  HCLE AKN+L+IETDLPGDV++HWG+C D+GK WEIP+EPYPPE
Sbjct: 279  VVKEILVDNSVSVSVRHCLETAKNLLYIETDLPGDVIVHWGICKDDGKTWEIPSEPYPPE 338

Query: 874  TIRFKNKALRTLLQRKDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTS 695
            T  FK+KALRTLLQRK+ G GS GSF LD+   AFVFVLKLNENTWLNC+GNDF IP +S
Sbjct: 339  TSMFKDKALRTLLQRKNGGHGSLGSFILDEGSVAFVFVLKLNENTWLNCHGNDFCIPLSS 398

Query: 694  SVATDEHSSLTHSKGEEHLKEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXX 515
                  HS   HS+ EE   E GS +     SE+N+ VSA+TD II+EIR+LV+DI    
Sbjct: 399  FSVPPIHSGQPHSEDEEQNNESGSLLISGNASEANQEVSAYTDEIIDEIRSLVTDISSEK 458

Query: 514  XXXXXXXEAQESILQEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGY 335
                   +AQESILQEIEKLAAEAYSIFRSS+PT+T+TD+ E++ L+           G+
Sbjct: 459  NRRTKSKKAQESILQEIEKLAAEAYSIFRSSIPTFTETDLLEEEALEPPVKISSGTGSGF 518

Query: 334  EILCQGFNWESHKSGKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNS 155
            EILCQGFNWESHKSGKWY++L EKAS+L+SLGFTV+WLPPPTDSVS EGYMPRDLYNLNS
Sbjct: 519  EILCQGFNWESHKSGKWYIDLREKASKLASLGFTVLWLPPPTDSVSSEGYMPRDLYNLNS 578

Query: 154  RYGNIDQLKSLVSKLHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            RYGN+D+LK LV +LHEVGI+VLGDVVLNHRCAQYKNQNG+WNIFGGRLNW
Sbjct: 579  RYGNMDELKILVKELHEVGIRVLGDVVLNHRCAQYKNQNGIWNIFGGRLNW 629


>ref|XP_012855272.1| PREDICTED: alpha-amylase 3, chloroplastic [Erythranthe guttata]
 gb|EYU22438.1| hypothetical protein MIMGU_mgv1a001104mg [Erythranthe guttata]
          Length = 888

 Score =  778 bits (2009), Expect = 0.0
 Identities = 399/629 (63%), Positives = 462/629 (73%), Gaps = 4/629 (0%)
 Frame = -3

Query: 1876 MSVVTTDRLFLHHHTCTTFKPVQKFQFISKKLQPFNPICPRPRTRR----ANICSRHFYI 1709
            MS V T+ LF H     + K + KF  IS+K   FN IC    T R      +CSR++  
Sbjct: 1    MSAVATELLFHHRVHRISPKLIPKFHLISRKSPTFNLICDTTTTDRRFVGTGLCSRYYKP 60

Query: 1708 PKALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLP 1529
             KA+SS+GA V+ETSESS +TFSETF L+R EK+EGKITI+LD G+ EECW+L VGCS+P
Sbjct: 61   LKAVSSAGAAVLETSESSTITFSETFQLERVEKVEGKITIKLDKGETEECWQLIVGCSIP 120

Query: 1528 GKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKI 1349
            GKW+LHWGVNY+ DVGSEWDQPPLDMRP GSIP+KDYAIETP E     +EGEAF EV I
Sbjct: 121  GKWILHWGVNYVSDVGSEWDQPPLDMRPPGSIPVKDYAIETPLESKSELAEGEAFSEVAI 180

Query: 1348 DFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGAL 1169
             F+T SSIA+INFVLKDEE+G WYQHRGRDFKVPLI++ Q D      K G      G +
Sbjct: 181  VFDTKSSIASINFVLKDEETGRWYQHRGRDFKVPLIEHPQID-----VKAG-----EGDV 230

Query: 1168 GQISNMLHKPEAADSKGDDIRESNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERA 989
             Q++ +L KPEAA+SKGDDI    LQ+ PLQ+FYEE SV KE +  NSISVS  H  E+ 
Sbjct: 231  AQLTTVLVKPEAAESKGDDIGGVRLQRGPLQSFYEEFSVVKETVNSNSISVSVKHTEEKD 290

Query: 988  KNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGS 809
            K +L+IETDLPGDVV+HWGVC DE K WEIP EPYPP TI FKNKALRTLLQRK+DG GS
Sbjct: 291  KCVLYIETDLPGDVVVHWGVCRDESKKWEIPVEPYPPGTIVFKNKALRTLLQRKNDGDGS 350

Query: 808  WGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEF 629
             GSFTL +    FVFVLKLNEN WLNC G+DFYIP   +V  D+ S              
Sbjct: 351  GGSFTLGEGLLGFVFVLKLNENAWLNCKGSDFYIPLPRTVVPDKLSV------------- 397

Query: 628  GSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAA 449
             SP+  +  SESN+  S +TDGII+EIR+LV+DI           + +ESILQEIEKLAA
Sbjct: 398  -SPLISENASESNQTSSTYTDGIISEIRSLVTDISSEKTRQTKNKKVRESILQEIEKLAA 456

Query: 448  EAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMELY 269
            EAYSIFRSS+PT    D++ED+ L+           G EI+CQGFNWESHKSG WY+EL 
Sbjct: 457  EAYSIFRSSIPTVPKIDLAEDEVLEPPVKVCSGTGSGNEIVCQGFNWESHKSGNWYIELQ 516

Query: 268  EKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKV 89
            EKAS+LS LGFTVIWLPPPTDSVSPEGYMP DLYNLNSRYG+IDQLK LV KLHEVGI V
Sbjct: 517  EKASKLSELGFTVIWLPPPTDSVSPEGYMPSDLYNLNSRYGSIDQLKVLVKKLHEVGIMV 576

Query: 88   LGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            LGD VLNHRCAQ++NQNGVWNIFGGRLNW
Sbjct: 577  LGDAVLNHRCAQFQNQNGVWNIFGGRLNW 605


>emb|CDP00291.1| unnamed protein product [Coffea canephora]
          Length = 894

 Score =  747 bits (1928), Expect = 0.0
 Identities = 385/634 (60%), Positives = 464/634 (73%), Gaps = 9/634 (1%)
 Frame = -3

Query: 1876 MSVVTTDRLFLHHHTCTTFKPVQKFQFISKKLQPFNPICPR-PRTRRANICSRHFYIPKA 1700
            MS V T+ L   H++      V      S++  P    C R P +   ++ S     PKA
Sbjct: 1    MSAVATELLV--HYSLRRRTLVPGIHAYSRRAPPLYLNCTRRPLSAGPSLSSFELKAPKA 58

Query: 1699 LSSSGATVVETSE--SSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPG 1526
            L+   +   +T+    S+V F ETF LKRP K+EGKITIRL NGK+EE W+L VGCSLPG
Sbjct: 59   LTLRASASTDTAAVVESDVVFGETFSLKRPVKVEGKITIRLVNGKDEENWQLIVGCSLPG 118

Query: 1525 KWVLHWGVNYIGDVG--SEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVK 1352
            KWVLHWGV YIGDVG  SEWDQPP +MRP GSI IKDYAIE+P E+S T  EGE+F+EVK
Sbjct: 119  KWVLHWGVKYIGDVGRCSEWDQPPPEMRPPGSIAIKDYAIESPLEKSSTL-EGESFYEVK 177

Query: 1351 IDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGA 1172
            IDFNT++SI AINFVLKDE+SG WYQHRGRDFKVPL +Y  DDGNVVGAKKG GIW  GA
Sbjct: 178  IDFNTNNSITAINFVLKDEDSGSWYQHRGRDFKVPLTEYRHDDGNVVGAKKGFGIWP-GA 236

Query: 1171 LGQISNMLHKPEAADSKGD----DIRESNLQKRPLQAFYEEHSVFKEIITDNSISVSASH 1004
             GQ+SNML K E A++K D    + +    Q   L+ FYEEH + +E + DNS++VS + 
Sbjct: 237  FGQLSNMLLKSEGAENKMDFTSCESKAPPQQNSRLEGFYEEHPIVRETLVDNSVTVSVTQ 296

Query: 1003 CLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKD 824
            C E AKN+L+IETDLPGDV++HWGVC D+G+ WE+P +PYP ET  FKNKALRTLLQRK+
Sbjct: 297  CPETAKNLLYIETDLPGDVIVHWGVCKDKGRKWELPEQPYPSETKVFKNKALRTLLQRKE 356

Query: 823  DGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEE 644
             G+ S GSFTLD   +AFVF LKLNENTWLN  G DFYIP +SS   ++  S +HS    
Sbjct: 357  GGTCSSGSFTLDVGLTAFVFALKLNENTWLNNMGKDFYIPLSSSRVLNKEHSQSHS---- 412

Query: 643  HLKEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEI 464
                          +++ E+ +A+TDGIINEIRNLVSDI           EAQESILQEI
Sbjct: 413  -------------ANKTKESSTAYTDGIINEIRNLVSDIASEKSRKTKIKEAQESILQEI 459

Query: 463  EKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKW 284
            EKLAAEAYSIFRS++PT+T+ +VSE + L+           G+E++CQGFNWESHKSG+W
Sbjct: 460  EKLAAEAYSIFRSAIPTFTEEEVSEAEVLKPSVKIASGTGSGFEVVCQGFNWESHKSGRW 519

Query: 283  YMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHE 104
            YMEL++KA+ELSSLGFTV+WLPPPT+SVSPEGYMP+DLYNLNSRYG+ID+LKSLV + HE
Sbjct: 520  YMELHQKAAELSSLGFTVVWLPPPTESVSPEGYMPKDLYNLNSRYGSIDELKSLVKRFHE 579

Query: 103  VGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            VGI VLGD VLNHRCA YKNQNG+WNIFGGRLNW
Sbjct: 580  VGIMVLGDAVLNHRCAHYKNQNGIWNIFGGRLNW 613


>ref|XP_009599231.1| PREDICTED: alpha-amylase 3, chloroplastic isoform X2 [Nicotiana
            tomentosiformis]
 ref|XP_016465752.1| PREDICTED: alpha-amylase 3, chloroplastic-like [Nicotiana tabacum]
          Length = 902

 Score =  743 bits (1917), Expect = 0.0
 Identities = 367/567 (64%), Positives = 432/567 (76%), Gaps = 2/567 (0%)
 Frame = -3

Query: 1696 SSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPGKWV 1517
            SS+ A V+ETSE S+V  +ETF +KRPE+ EGKI IRLD GK+EE W L+VGCS+PGKW+
Sbjct: 59   SSTDAAVIETSEQSDVVLTETFSVKRPERAEGKIAIRLDKGKDEENWHLSVGCSVPGKWI 118

Query: 1516 LHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKIDFNT 1337
            LHWGV+Y GD GSEWDQPP +MRPSGSI IKDYAIETP + S T  EGE F EVKID  +
Sbjct: 119  LHWGVHYAGDAGSEWDQPPPEMRPSGSIAIKDYAIETPLKGSSTILEGEKFQEVKIDIRS 178

Query: 1336 SSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALGQIS 1157
            + SIAAINFVLKDEE+G WYQHRGRDFK+PLIDYL+DD N+VG KKG GIW  G LGQ+S
Sbjct: 179  TWSIAAINFVLKDEETGVWYQHRGRDFKIPLIDYLEDDANIVGVKKGSGIWP-GPLGQLS 237

Query: 1156 NMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERAKN 983
            N+L KPEAA SKG+      S+++ + L+ FYE H++ KE + DN++SVS  HC E  KN
Sbjct: 238  NILLKPEAAPSKGEASSNDGSSVKSKRLEGFYEVHTIVKETLVDNTVSVSVKHCPETKKN 297

Query: 982  ILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGSWG 803
            IL+I+TDLPG+VV+HWGVC D+ K WE+P  PYP ET+ FKNKALRTLLQRK+ G+GS G
Sbjct: 298  ILYIDTDLPGNVVLHWGVCKDDAKTWELPTMPYPAETVVFKNKALRTLLQRKEGGNGSSG 357

Query: 802  SFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFGS 623
             FTLD   S FVFV+KL+ENTWLNC GNDFYIP +  +       L  +K +E   E  S
Sbjct: 358  LFTLDGGLSGFVFVVKLDENTWLNCKGNDFYIPLSGGIYQSSTKHLEETKQDE---ESNS 414

Query: 622  PVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAAEA 443
               ++   E  +A S  TD II EIRNLV+DI           E QE+ILQEIEKLAAEA
Sbjct: 415  SEILNRAPEEKQAGSVFTDEIIKEIRNLVTDISSEKSRKTKSKETQENILQEIEKLAAEA 474

Query: 442  YSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMELYEK 263
            YSIFRSS+PT  +  VSE +  Q           G+EILCQGFNWESHKSG+WY+EL+EK
Sbjct: 475  YSIFRSSIPTIPENVVSEAEVSQPDVKVSSGTGSGFEILCQGFNWESHKSGRWYVELHEK 534

Query: 262  ASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKVLG 83
            A+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG+ D+LK  V K HEVGIKVLG
Sbjct: 535  ATELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSFDELKVTVKKFHEVGIKVLG 594

Query: 82   DVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            DVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 595  DVVLNHRCASERNQNGIWNIFGGRLNW 621


>ref|XP_009599230.1| PREDICTED: alpha-amylase 3, chloroplastic isoform X1 [Nicotiana
            tomentosiformis]
          Length = 903

 Score =  738 bits (1905), Expect = 0.0
 Identities = 367/568 (64%), Positives = 432/568 (76%), Gaps = 3/568 (0%)
 Frame = -3

Query: 1696 SSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPGKWV 1517
            SS+ A V+ETSE S+V  +ETF +KRPE+ EGKI IRLD GK+EE W L+VGCS+PGKW+
Sbjct: 59   SSTDAAVIETSEQSDVVLTETFSVKRPERAEGKIAIRLDKGKDEENWHLSVGCSVPGKWI 118

Query: 1516 LHWGVNYIGDVGS-EWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKIDFN 1340
            LHWGV+Y GD GS EWDQPP +MRPSGSI IKDYAIETP + S T  EGE F EVKID  
Sbjct: 119  LHWGVHYAGDAGSSEWDQPPPEMRPSGSIAIKDYAIETPLKGSSTILEGEKFQEVKIDIR 178

Query: 1339 TSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALGQI 1160
            ++ SIAAINFVLKDEE+G WYQHRGRDFK+PLIDYL+DD N+VG KKG GIW  G LGQ+
Sbjct: 179  STWSIAAINFVLKDEETGVWYQHRGRDFKIPLIDYLEDDANIVGVKKGSGIWP-GPLGQL 237

Query: 1159 SNMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERAK 986
            SN+L KPEAA SKG+      S+++ + L+ FYE H++ KE + DN++SVS  HC E  K
Sbjct: 238  SNILLKPEAAPSKGEASSNDGSSVKSKRLEGFYEVHTIVKETLVDNTVSVSVKHCPETKK 297

Query: 985  NILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGSW 806
            NIL+I+TDLPG+VV+HWGVC D+ K WE+P  PYP ET+ FKNKALRTLLQRK+ G+GS 
Sbjct: 298  NILYIDTDLPGNVVLHWGVCKDDAKTWELPTMPYPAETVVFKNKALRTLLQRKEGGNGSS 357

Query: 805  GSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFG 626
            G FTLD   S FVFV+KL+ENTWLNC GNDFYIP +  +       L  +K +E   E  
Sbjct: 358  GLFTLDGGLSGFVFVVKLDENTWLNCKGNDFYIPLSGGIYQSSTKHLEETKQDE---ESN 414

Query: 625  SPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAAE 446
            S   ++   E  +A S  TD II EIRNLV+DI           E QE+ILQEIEKLAAE
Sbjct: 415  SSEILNRAPEEKQAGSVFTDEIIKEIRNLVTDISSEKSRKTKSKETQENILQEIEKLAAE 474

Query: 445  AYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMELYE 266
            AYSIFRSS+PT  +  VSE +  Q           G+EILCQGFNWESHKSG+WY+EL+E
Sbjct: 475  AYSIFRSSIPTIPENVVSEAEVSQPDVKVSSGTGSGFEILCQGFNWESHKSGRWYVELHE 534

Query: 265  KASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKVL 86
            KA+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG+ D+LK  V K HEVGIKVL
Sbjct: 535  KATELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSFDELKVTVKKFHEVGIKVL 594

Query: 85   GDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            GDVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 595  GDVVLNHRCASERNQNGIWNIFGGRLNW 622


>ref|XP_009758349.1| PREDICTED: alpha-amylase 3, chloroplastic isoform X2 [Nicotiana
            sylvestris]
 ref|XP_016456173.1| PREDICTED: alpha-amylase 3, chloroplastic-like isoform X2 [Nicotiana
            tabacum]
          Length = 905

 Score =  736 bits (1899), Expect = 0.0
 Identities = 369/570 (64%), Positives = 431/570 (75%), Gaps = 5/570 (0%)
 Frame = -3

Query: 1696 SSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPGKWV 1517
            SS+ A V+ETSE S+V  +ETF +KRPE+ EGKI IRLD GK+EE W L+VGCS+PGKW+
Sbjct: 59   SSTDAAVIETSEKSDVVLTETFLVKRPERAEGKIAIRLDKGKDEENWHLSVGCSVPGKWI 118

Query: 1516 LHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKIDFNT 1337
            LHWGVNY GD GSEWDQPP +MRPSGSI IKDYAIETP + S T  EGE F EVKID ++
Sbjct: 119  LHWGVNYAGDAGSEWDQPPPEMRPSGSITIKDYAIETPLKGSSTILEGEKFQEVKIDISS 178

Query: 1336 SSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALGQIS 1157
              SIAAINFVLKDEE+G WYQHRGRDFK+PLIDYL+DD N+VG KKG GIW  G LGQ+S
Sbjct: 179  KWSIAAINFVLKDEETGVWYQHRGRDFKIPLIDYLEDDANIVGVKKGSGIW-PGPLGQLS 237

Query: 1156 NMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERAKN 983
            N+L KPEAA SKG+      S+L+ + L+ FYEE ++ KE   DN++SV   HC + AKN
Sbjct: 238  NILLKPEAAPSKGEASSNDGSSLKNKRLEGFYEEQTIVKETFVDNTVSVIVKHCPDTAKN 297

Query: 982  ILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGSWG 803
            IL+I+TDLPG+VV+HWGVC D+ K WE+P  PYP ET+ FKNKALRTLLQRK+ G GS G
Sbjct: 298  ILYIDTDLPGNVVLHWGVCKDDAKTWELPTTPYPAETVVFKNKALRTLLQRKEGGDGSSG 357

Query: 802  SFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFGS 623
             FTLD   S FVFV+KL+ENTWLNC GNDFYIP +S       SS  HS+  +  +E  S
Sbjct: 358  LFTLDGGLSGFVFVVKLDENTWLNCKGNDFYIPLSSG---KYQSSTIHSEERKQDEESNS 414

Query: 622  PVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAAEA 443
               ++   E  +A S  TD II EIRNLV+ I           E QE+ILQEIEKLAAEA
Sbjct: 415  SEILNRAPEEKQAGSMFTDEIIKEIRNLVTGISSEKSRKTKSKETQENILQEIEKLAAEA 474

Query: 442  YSIFRSS---MPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMEL 272
            YSIFRSS   +PT ++  VSE +  Q           G+EILCQGFNWESHKSG+WY EL
Sbjct: 475  YSIFRSSTPTVPTISENVVSEAEVSQPDVKVSSGTGSGFEILCQGFNWESHKSGRWYREL 534

Query: 271  YEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIK 92
            +EKA+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG+ D+LK  V K HEVGIK
Sbjct: 535  HEKAAELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSFDELKVTVKKFHEVGIK 594

Query: 91   VLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            VLGDVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 595  VLGDVVLNHRCASERNQNGIWNIFGGRLNW 624


>gb|AAX33233.1| plastid alpha-amylase [Actinidia chinensis]
          Length = 895

 Score =  731 bits (1886), Expect = 0.0
 Identities = 358/592 (60%), Positives = 451/592 (76%), Gaps = 7/592 (1%)
 Frame = -3

Query: 1756 RPRTRRANICSRHFYIPKALS----SSGATVVETSESSNVTFSETFHLKRPEKLEGKITI 1589
            RP +  ++ C+  F  P+ LS    S+   VVETS+S +V F ETF LKR EK+EG I+I
Sbjct: 37   RPLSHGSSFCN--FRPPQPLSVRASSADTAVVETSDSVDVLFKETFALKRIEKVEGHISI 94

Query: 1588 RLDNGKNEECWKLTVGCSLPGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIE 1409
            +LDNGK  E W+L+VGC+LPGKWVLHWGVNYI D+GSEWDQPP++MRP GS+PIKDYAIE
Sbjct: 95   KLDNGKERENWQLSVGCNLPGKWVLHWGVNYINDIGSEWDQPPVEMRPPGSVPIKDYAIE 154

Query: 1408 TPFERSPTSSEGEAFHEVKIDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQ 1229
            TP ++S    EG+ ++E+KIDF+T   IAAINFVLKDEE+G WYQ RGRDFKV LID L 
Sbjct: 155  TPLKKSSAVVEGDLYYELKIDFSTDKDIAAINFVLKDEETGAWYQRRGRDFKVXLIDXLH 214

Query: 1228 DDGNVVGAKKGLGIWQAGALGQISNMLHKPEAADSKGDDI---RESNLQKRPLQAFYEEH 1058
            +DGN +GAKKGLG+   G   Q+S++L K E A  KG+D    R+ +   + L+AFYEEH
Sbjct: 215  EDGNKLGAKKGLGVXP-GPFEQLSSLLLKSEEAHPKGEDSSDSRDPSKTTKCLEAFYEEH 273

Query: 1057 SVFKEIITDNSISVSASHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPP 878
            S+ +E++ +NS+SVSA  C + AKN+LHIETD+PGDVV+HWG+C D+G+NWEIPA+PYP 
Sbjct: 274  SIVREVLINNSVSVSARKCPKTAKNLLHIETDIPGDVVVHWGLCKDDGENWEIPAKPYPA 333

Query: 877  ETIRFKNKALRTLLQRKDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFT 698
            ETI FKNKALRTLL+ K+ G G W  FTLD+ ++ FVFVLK+NENTWLN  GNDFYIP +
Sbjct: 334  ETIVFKNKALRTLLKXKEGGKGGWSLFTLDEGYAGFVFVLKINENTWLNYMGNDFYIPLS 393

Query: 697  SSVATDEHSSLTHSKGEEHLKEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXX 518
            SS          H + E H +         + ++   + +A+TDGIIN+IR+LVSDI   
Sbjct: 394  SSSVLPAQPR--HDQSEGHXQ---------VETDQEVSPAAYTDGIINDIRSLVSDISSX 442

Query: 517  XXXXXXXXEAQESILQEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXG 338
                    E+Q+SILQEIEKLAAEAYSIFRSS+PTY +  + E ++++           G
Sbjct: 443  KSRQTKSKESQQSILQEIEKLAAEAYSIFRSSIPTYXEDVMVESEEVEPPAKISSGTGSG 502

Query: 337  YEILCQGFNWESHKSGKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLN 158
            +EILCQGFNWESHKSG+WYM+L E+A+E+SS+GFTV+WLPPPT+SVSPEGYMP DLYNLN
Sbjct: 503  FEILCQGFNWESHKSGRWYMQLLERAAEISSIGFTVVWLPPPTESVSPEGYMPGDLYNLN 562

Query: 157  SRYGNIDQLKSLVSKLHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            SRYGN+++LK +V + HEVGI+VLGDVVLNHRCAQYKNQNG+WNIFGGRLNW
Sbjct: 563  SRYGNVEELKLIVKRFHEVGIRVLGDVVLNHRCAQYKNQNGIWNIFGGRLNW 614


>ref|XP_009758348.1| PREDICTED: alpha-amylase 3, chloroplastic isoform X1 [Nicotiana
            sylvestris]
 ref|XP_016456172.1| PREDICTED: alpha-amylase 3, chloroplastic-like isoform X1 [Nicotiana
            tabacum]
          Length = 906

 Score =  731 bits (1887), Expect = 0.0
 Identities = 369/571 (64%), Positives = 431/571 (75%), Gaps = 6/571 (1%)
 Frame = -3

Query: 1696 SSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPGKWV 1517
            SS+ A V+ETSE S+V  +ETF +KRPE+ EGKI IRLD GK+EE W L+VGCS+PGKW+
Sbjct: 59   SSTDAAVIETSEKSDVVLTETFLVKRPERAEGKIAIRLDKGKDEENWHLSVGCSVPGKWI 118

Query: 1516 LHWGVNYIGDVGS-EWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKIDFN 1340
            LHWGVNY GD GS EWDQPP +MRPSGSI IKDYAIETP + S T  EGE F EVKID +
Sbjct: 119  LHWGVNYAGDAGSSEWDQPPPEMRPSGSITIKDYAIETPLKGSSTILEGEKFQEVKIDIS 178

Query: 1339 TSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALGQI 1160
            +  SIAAINFVLKDEE+G WYQHRGRDFK+PLIDYL+DD N+VG KKG GIW  G LGQ+
Sbjct: 179  SKWSIAAINFVLKDEETGVWYQHRGRDFKIPLIDYLEDDANIVGVKKGSGIWP-GPLGQL 237

Query: 1159 SNMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERAK 986
            SN+L KPEAA SKG+      S+L+ + L+ FYEE ++ KE   DN++SV   HC + AK
Sbjct: 238  SNILLKPEAAPSKGEASSNDGSSLKNKRLEGFYEEQTIVKETFVDNTVSVIVKHCPDTAK 297

Query: 985  NILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGSW 806
            NIL+I+TDLPG+VV+HWGVC D+ K WE+P  PYP ET+ FKNKALRTLLQRK+ G GS 
Sbjct: 298  NILYIDTDLPGNVVLHWGVCKDDAKTWELPTTPYPAETVVFKNKALRTLLQRKEGGDGSS 357

Query: 805  GSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFG 626
            G FTLD   S FVFV+KL+ENTWLNC GNDFYIP +S       SS  HS+  +  +E  
Sbjct: 358  GLFTLDGGLSGFVFVVKLDENTWLNCKGNDFYIPLSSG---KYQSSTIHSEERKQDEESN 414

Query: 625  SPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAAE 446
            S   ++   E  +A S  TD II EIRNLV+ I           E QE+ILQEIEKLAAE
Sbjct: 415  SSEILNRAPEEKQAGSMFTDEIIKEIRNLVTGISSEKSRKTKSKETQENILQEIEKLAAE 474

Query: 445  AYSIFRSS---MPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYME 275
            AYSIFRSS   +PT ++  VSE +  Q           G+EILCQGFNWESHKSG+WY E
Sbjct: 475  AYSIFRSSTPTVPTISENVVSEAEVSQPDVKVSSGTGSGFEILCQGFNWESHKSGRWYRE 534

Query: 274  LYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGI 95
            L+EKA+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG+ D+LK  V K HEVGI
Sbjct: 535  LHEKAAELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSFDELKVTVKKFHEVGI 594

Query: 94   KVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            KVLGDVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 595  KVLGDVVLNHRCASERNQNGIWNIFGGRLNW 625


>gb|AFO84072.1| alpha-amylase [Actinidia chinensis]
          Length = 895

 Score =  730 bits (1884), Expect = 0.0
 Identities = 356/592 (60%), Positives = 454/592 (76%), Gaps = 7/592 (1%)
 Frame = -3

Query: 1756 RPRTRRANICSRHFYIPKALS----SSGATVVETSESSNVTFSETFHLKRPEKLEGKITI 1589
            RP +  ++ C+  F  P+ LS    S+   VVETS+S +V F ETF LKR EK+EG I+I
Sbjct: 37   RPLSHGSSFCN--FRPPQPLSVRASSADTAVVETSDSVDVLFKETFALKRIEKVEGNISI 94

Query: 1588 RLDNGKNEECWKLTVGCSLPGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIE 1409
            +LDNGK+ E W+L+VGC+LPGKWVLHWGVNYI D+GSEWDQPP++MRP GS+PIKDYAIE
Sbjct: 95   KLDNGKDRENWQLSVGCNLPGKWVLHWGVNYINDIGSEWDQPPVEMRPPGSVPIKDYAIE 154

Query: 1408 TPFERSPTSSEGEAFHEVKIDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQ 1229
            TP ++S    EG+ ++E+KIDF+T + IAAINFVLKDEE+G WYQ RGRDFKV LID L 
Sbjct: 155  TPLKKSSAVVEGDLYYELKIDFSTDTDIAAINFVLKDEETGAWYQRRGRDFKVALIDDLH 214

Query: 1228 DDGNVVGAKKGLGIWQAGALGQISNMLHKPEAADSKGDDIRES---NLQKRPLQAFYEEH 1058
            +DG+ +GAKKGLG+ + G   Q+S++L K E A  KG+D  +S   + + + L+ FYEEH
Sbjct: 215  EDGSKLGAKKGLGV-RPGPFEQLSSLLLKSEEAHPKGEDNSDSRGPSKKTKCLEGFYEEH 273

Query: 1057 SVFKEIITDNSISVSASHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPP 878
            S+ KE++ +NS+SVSA  C + AKN+LHIETD+PGDVV+HWG+C ++G+ WEIPA+PYP 
Sbjct: 274  SIVKEVLINNSVSVSARKCPKTAKNLLHIETDIPGDVVVHWGLCKEDGEKWEIPAKPYPA 333

Query: 877  ETIRFKNKALRTLLQRKDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFT 698
            ETI FKNKALRTLLQRK+ G G W  FTLD+ ++ FVFVLK+NENTWLN  GNDFYIP +
Sbjct: 334  ETIVFKNKALRTLLQRKEGGKGGWSLFTLDEGYAGFVFVLKINENTWLNYMGNDFYIPLS 393

Query: 697  SSVATDEHSSLTHSKGEEHLKEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXX 518
            SS          H + E H +         + ++   + +A+TDGIIN+IR+LVSDI   
Sbjct: 394  SSSVLPAQPR--HDQSEGHRQ---------VETDQEVSPAAYTDGIINDIRSLVSDISSG 442

Query: 517  XXXXXXXXEAQESILQEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXG 338
                    E+Q+SILQEIEKLAAEAYSIFRSS+PTY++  + E ++++           G
Sbjct: 443  KSRQTKSKESQQSILQEIEKLAAEAYSIFRSSIPTYSEDVMVESEEVEPPAKISSGTGSG 502

Query: 337  YEILCQGFNWESHKSGKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLN 158
            +EILCQGFNWESHKSG+WYM+L E+A+E+SS+GFTV+WLPPPT+SVSPEGYMP DLYNLN
Sbjct: 503  FEILCQGFNWESHKSGRWYMQLLERAAEISSIGFTVVWLPPPTESVSPEGYMPGDLYNLN 562

Query: 157  SRYGNIDQLKSLVSKLHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            SRYGN+++LK +V + HEVGI+VLGDVVLNHRCAQYKNQNG+WNIFGGRLNW
Sbjct: 563  SRYGNVEELKLIVKRFHEVGIRVLGDVVLNHRCAQYKNQNGIWNIFGGRLNW 614


>ref|XP_019256898.1| PREDICTED: alpha-amylase 3, chloroplastic [Nicotiana attenuata]
 gb|OIS95831.1| alpha-amylase 3, chloroplastic [Nicotiana attenuata]
          Length = 898

 Score =  727 bits (1877), Expect = 0.0
 Identities = 362/567 (63%), Positives = 429/567 (75%), Gaps = 2/567 (0%)
 Frame = -3

Query: 1696 SSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPGKWV 1517
            SS+   V+ETSE S+V  +ETF +KRPE+ EGKI I+LD GK+EE W L+VGCS+PGKW+
Sbjct: 59   SSTDTAVMETSEQSDVVLTETFSVKRPERAEGKIAIKLDKGKDEENWHLSVGCSVPGKWI 118

Query: 1516 LHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKIDFNT 1337
            LHWGV+Y GD GSEWDQPP +MRPSGSI IKDYAIETP + S T  EGE F EVKID  +
Sbjct: 119  LHWGVHYAGDAGSEWDQPPPEMRPSGSIAIKDYAIETPLKGSSTILEGETFQEVKIDICS 178

Query: 1336 SSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALGQIS 1157
              SIAAINFVLKDEE+G WYQHRGRDFK+PL DYL DD NVVG KKG     +G LGQ+S
Sbjct: 179  KWSIAAINFVLKDEETGVWYQHRGRDFKIPLFDYLDDDANVVGVKKG-----SGPLGQLS 233

Query: 1156 NMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERAKN 983
            N+L KPEAA SKG+      S+++ + L+ F+EEH + KE + DN++SVS  HC E AKN
Sbjct: 234  NILLKPEAATSKGESSSSDGSSVKSKCLEGFFEEHVIVKETLVDNTVSVSVKHCPETAKN 293

Query: 982  ILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGSWG 803
            IL I+TDLPG+VV+HWGVC D+ K WE+PA PYP ET+ FKNKALRTLLQRK+ G+GS G
Sbjct: 294  ILCIDTDLPGNVVLHWGVCKDDAKTWELPATPYPAETVVFKNKALRTLLQRKEGGNGSSG 353

Query: 802  SFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFGS 623
             FTLD   S FVFV+KL ENTWLNC GNDFYIP ++ +     SS  H +  +  +E  S
Sbjct: 354  LFTLDGGLSGFVFVVKLGENTWLNCKGNDFYIPLSNGI---HPSSTIHLEERKQDEESNS 410

Query: 622  PVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLAAEA 443
               ++   E  +A S +TD II EIRNLV+ I           E QE+ILQEIEKLAAEA
Sbjct: 411  SEILNRAPEEKQAGSVYTDEIIKEIRNLVTGISSEKSRRTKSKETQENILQEIEKLAAEA 470

Query: 442  YSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMELYEK 263
            YSIFRSS+PT ++  VSE +  Q           G+EILCQGFNWESHKSG+WYMEL+EK
Sbjct: 471  YSIFRSSIPTMSEIVVSEAEISQPDIKVSSGTGTGFEILCQGFNWESHKSGRWYMELHEK 530

Query: 262  ASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKVLG 83
            A+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG+ ++LK  V K H+VGIKVLG
Sbjct: 531  AAELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSFEELKVTVKKFHKVGIKVLG 590

Query: 82   DVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            DVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 591  DVVLNHRCASERNQNGIWNIFGGRLNW 617


>ref|XP_019170778.1| PREDICTED: alpha-amylase 3, chloroplastic isoform X1 [Ipomoea nil]
          Length = 904

 Score =  726 bits (1874), Expect = 0.0
 Identities = 382/635 (60%), Positives = 464/635 (73%), Gaps = 10/635 (1%)
 Frame = -3

Query: 1876 MSVVTTDRLFLHHHTCTTFKPVQKFQFISKKLQPFNP-ICPRPRT---RRANICS-RHFY 1712
            MS VT + L +HH    +F+   KF    ++  P +     RP +    R + C+ +H  
Sbjct: 1    MSTVTLEPL-IHH----SFRRTPKFCLNHRQPSPSSVNYTRRPLSVGAARLSFCNCKHRP 55

Query: 1711 IPKALS-SSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCS 1535
              KAL+  + +TV+ETSE   V F ETF LKR +++EG+I+IR D+G++EE WKLT+GC+
Sbjct: 56   RRKALAVRASSTVIETSE---VVFEETFDLKRTQRVEGRISIRFDSGRDEENWKLTIGCN 112

Query: 1534 LPGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEV 1355
            LPGKWVLHWGV+Y+GD GSEWDQPPL+MRP GSI IKDYAIETP +R  T+ EGE+F+EV
Sbjct: 113  LPGKWVLHWGVHYVGDSGSEWDQPPLEMRPQGSITIKDYAIETPLKRLSTALEGESFYEV 172

Query: 1354 KIDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAG 1175
            +I  N +S IAAINFVLKDEE+G WYQHRG DF+VPL+DY  DD N+VG KKG GIW  G
Sbjct: 173  EIGLNINSQIAAINFVLKDEETGAWYQHRGMDFRVPLMDYAHDDSNMVGGKKGFGIW-TG 231

Query: 1174 ALGQISNMLHKPEAADSKGDD----IRESNLQKRPLQAFYEEHSVFKEIITDNSISVSAS 1007
            ALGQ+S+ML K E   +KG++      E   Q R L  FYEEH + KE + DNS++VS  
Sbjct: 232  ALGQLSDMLLKSEVDHTKGENGSNGSSEPREQTRCLAEFYEEHVIVKETLVDNSVTVSVK 291

Query: 1006 HCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRK 827
               E AKN+L I+TD+PGDV+IHWGVC DEGKNWE+PA+PYP ET  FKNKALRTLLQ+K
Sbjct: 292  EYPETAKNLLQIDTDIPGDVLIHWGVCRDEGKNWELPAKPYPTETTIFKNKALRTLLQQK 351

Query: 826  DDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGE 647
            DDGSGS  SFTLD+    FVFVLKL++ TWLNC GNDFY+P   S  T    S T S+ E
Sbjct: 352  DDGSGSQRSFTLDEGPVGFVFVLKLDDGTWLNCKGNDFYVPLPRS--TKGQLSATESEVE 409

Query: 646  EHLKEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQE 467
               KE  S  +   TSE+ EA S +TD IINEIRNLV+DI           EAQE ILQE
Sbjct: 410  TQNKELDSLGASGSTSEAMEA-SLYTDEIINEIRNLVTDISSEKNRNTKTKEAQEGILQE 468

Query: 466  IEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGK 287
            IEKLAAEAYSIFRSS+PT++++ + E + L+           G+EILCQGFNWESHKSG+
Sbjct: 469  IEKLAAEAYSIFRSSVPTFSESALLEAEALKPPTKISSGTGSGHEILCQGFNWESHKSGR 528

Query: 286  WYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLH 107
            WY+EL EKA  LSSLG TV+WLPPPT+SVSPEGYMP+DLYNLNSRYG ID+LK +V + H
Sbjct: 529  WYLELQEKAELLSSLGITVVWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKVIVKRFH 588

Query: 106  EVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            EVGI+VLGDVVLNHRCA  +NQNGVWNIFGGRLNW
Sbjct: 589  EVGIQVLGDVVLNHRCASSRNQNGVWNIFGGRLNW 623


>ref|XP_023928052.1| alpha-amylase 3, chloroplastic isoform X2 [Quercus suber]
          Length = 912

 Score =  717 bits (1851), Expect = 0.0
 Identities = 369/637 (57%), Positives = 450/637 (70%), Gaps = 22/637 (3%)
 Frame = -3

Query: 1846 LHHHTCTTFKPVQKFQFISKKLQPFNPIC-PRPRTRRAN---ICSRHF----------YI 1709
            L HH+    +   K+   SK  +PF+  C P+ +T+        SR F           +
Sbjct: 9    LRHHS---LRENPKYSPKSKAFKPFSLNCSPQTQTQTQTKLFFHSRRFCNFRAPKHSHVV 65

Query: 1708 PKALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLP 1529
             KA S++   VV+T ES+ + F ETF LKR E +EGKI +RLD  KN + W+LTVGC+LP
Sbjct: 66   VKAASAAETAVVQTFESAELFFKETFPLKRTETVEGKIFVRLDQVKNSQIWQLTVGCNLP 125

Query: 1528 GKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKI 1349
            GKW+LHWGV+Y+GDVGSEWDQPP  MRP  SIPIKDYAIETP ++S +SS G+ F+EVKI
Sbjct: 126  GKWILHWGVSYVGDVGSEWDQPPSQMRPPDSIPIKDYAIETPMKKS-SSSGGDGFYEVKI 184

Query: 1348 DFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDG---NVVGAKKGLGIWQA 1178
            D + +S+IAAINFVLKD+E+G WYQHRGRDFKVPL+DY+Q DG   N+ GAK G  IW  
Sbjct: 185  DLSPNSAIAAINFVLKDDETGTWYQHRGRDFKVPLVDYVQGDGPDSNIAGAKWGFSIWP- 243

Query: 1177 GALGQISNMLHKPEAADSKGDD----IRESNLQKRPLQAFYEEHSVFKEIITDNSISVSA 1010
            GA GQ+S+++ K EA+ + G D     R+   + R L+ FY+E  + K++  DN+++VS 
Sbjct: 244  GAFGQLSSLILKGEASTTTGPDNSTNSRDPKQESRSLERFYDEQPIAKQVPVDNTVTVSV 303

Query: 1009 SHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQR 830
              C E AKN LH+ETDLPGDVVIHWGVC+D  K WEIPA PYPPETI FK+KALRT LQ 
Sbjct: 304  RKCPETAKNHLHVETDLPGDVVIHWGVCLDGAKKWEIPAAPYPPETIAFKDKALRTRLQP 363

Query: 829  KDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKG 650
            K+ G G WGSFT+++    F+FVLKLNE+TWLNC GNDFYIP +SS  +   +       
Sbjct: 364  KEGGDGCWGSFTVEEGLEGFLFVLKLNESTWLNCMGNDFYIPLSSSSLSARSTQSLSG-- 421

Query: 649  EEHLKEFGSPVSVDITSESNE-AVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESIL 473
                   G+ +   I  E+ E ++S +T GII EIRNLV DI           E QESIL
Sbjct: 422  -------GTQIPEKIAKENQEGSLSTYTSGIIKEIRNLVGDISSEKSQKTKSKEIQESIL 474

Query: 472  QEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKS 293
            +EIEKLAAEAYSIFRSS+PT  +  V E K+ +           G+EILCQGFNWESHKS
Sbjct: 475  REIEKLAAEAYSIFRSSVPTVIEEAVVEPKEEKAPVKICPGTGTGFEILCQGFNWESHKS 534

Query: 292  GKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSK 113
            G+WYMEL EKA ELSS+GFTVIWLPPPT+S+SPEGYMPRDLYNLNSRYGNIDQLK +V K
Sbjct: 535  GRWYMELKEKAEELSSIGFTVIWLPPPTESISPEGYMPRDLYNLNSRYGNIDQLKEVVKK 594

Query: 112  LHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
             HEVGIKVLGD VLNHRCA YKNQNGVWNI+GGRLNW
Sbjct: 595  FHEVGIKVLGDAVLNHRCAHYKNQNGVWNIYGGRLNW 631


>ref|XP_010256483.1| PREDICTED: alpha-amylase 3, chloroplastic-like [Nelumbo nucifera]
          Length = 924

 Score =  717 bits (1850), Expect = 0.0
 Identities = 368/649 (56%), Positives = 463/649 (71%), Gaps = 24/649 (3%)
 Frame = -3

Query: 1876 MSVVTTDRLFLHHHTCTTFKPVQKFQFISKKLQPFNP-ICPRPRTRRANICSRHFYIPKA 1700
            MS VT + L    H C   + +  F+  SKKL+P      P+    R   C+   Y  + 
Sbjct: 1    MSTVTLEPLL---HQCCRQRVI--FRLESKKLRPSRVNYSPKLCYHRRCFCNSKPYRFRT 55

Query: 1699 L--SSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPG 1526
            +  SS+ A +VE SE+++V+F ETF LKR E++EGKI++RLD GK+EE W+LTVGC LPG
Sbjct: 56   VRSSSTDAALVEASEAADVSFRETFQLKRTERVEGKISVRLDPGKDEENWQLTVGCDLPG 115

Query: 1525 KWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKID 1346
            KW+LHWGVNYI DVGSEWDQPP +M P GSIPIKDYAIETP ++S ++SEGE FHE KI 
Sbjct: 116  KWLLHWGVNYIDDVGSEWDQPPPEMIPPGSIPIKDYAIETPLKKSSSTSEGETFHEAKIK 175

Query: 1345 FNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALG 1166
            FN +SSI AINFVLKDEESG W QHRGRD+KVPLI YL +D N++GAKK  GIW  GALG
Sbjct: 176  FNCNSSIVAINFVLKDEESGAWCQHRGRDYKVPLISYLHEDANIIGAKKSFGIWP-GALG 234

Query: 1165 QISNMLHKPEAADSKGDDIR-ESNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLERA 989
            QI ++L KPE    + D    +   Q + L+ FYEEH +FKE+   N ++VS   C ++ 
Sbjct: 235  QIPSILLKPEKPTHEEDTGETDDKKQNKCLEGFYEEHPIFKEVPVQNYMTVSVRKCPDKD 294

Query: 988  KNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSGS 809
            KN++H++TDLPGDV++HWGVC D+ K WEIPA P+PP+T  FK KALRTLLQ K+DG G 
Sbjct: 295  KNLIHLDTDLPGDVIVHWGVCRDDDKKWEIPAAPHPPQTQVFKKKALRTLLQPKEDGHGC 354

Query: 808  WGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGE---EHL 638
            WG F+LD+ F A +FVLKLNENTWLN  G DFY+P + + ++   SS + ++G+   + L
Sbjct: 355  WGLFSLDREFKALLFVLKLNENTWLNYMGCDFYVPLSKANSSPVQSSQSQTEGQGKQDIL 414

Query: 637  KEFGSPVSVDITSESNEAVSA-----------------HTDGIINEIRNLVSDIXXXXXX 509
                S VS  + +E +E+ S+                 +TDGIINEIRNLVSDI      
Sbjct: 415  YLPKSEVSEVVINERDESSSSGISGKMADADKVVAQGGYTDGIINEIRNLVSDISSEKSH 474

Query: 508  XXXXXEAQESILQEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEI 329
                 E QE IL+EIEKLAAEAYSIFRSS PT+ +  +S+ + L+           GYEI
Sbjct: 475  KTKNKEVQEIILEEIEKLAAEAYSIFRSSTPTFLEEAISDAETLKPPLKICSGTGSGYEI 534

Query: 328  LCQGFNWESHKSGKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRY 149
            LCQGFNWESHKSG+WYMEL E+ASELSSLGFT++WLPPPT+SVSPEGYMP+DLYNLNSRY
Sbjct: 535  LCQGFNWESHKSGRWYMELTERASELSSLGFTILWLPPPTESVSPEGYMPKDLYNLNSRY 594

Query: 148  GNIDQLKSLVSKLHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            G+ ++LK +V   H+VGIKVLGDVVLNHRCA Y+N++GVWNIFGG+LNW
Sbjct: 595  GSTEELKLVVKCFHQVGIKVLGDVVLNHRCAHYQNKSGVWNIFGGKLNW 643


>gb|POE91280.1| alpha-amylase 3, chloroplastic [Quercus suber]
          Length = 954

 Score =  717 bits (1851), Expect = 0.0
 Identities = 369/637 (57%), Positives = 450/637 (70%), Gaps = 22/637 (3%)
 Frame = -3

Query: 1846 LHHHTCTTFKPVQKFQFISKKLQPFNPIC-PRPRTRRAN---ICSRHF----------YI 1709
            L HH+    +   K+   SK  +PF+  C P+ +T+        SR F           +
Sbjct: 9    LRHHS---LRENPKYSPKSKAFKPFSLNCSPQTQTQTQTKLFFHSRRFCNFRAPKHSHVV 65

Query: 1708 PKALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLP 1529
             KA S++   VV+T ES+ + F ETF LKR E +EGKI +RLD  KN + W+LTVGC+LP
Sbjct: 66   VKAASAAETAVVQTFESAELFFKETFPLKRTETVEGKIFVRLDQVKNSQIWQLTVGCNLP 125

Query: 1528 GKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKI 1349
            GKW+LHWGV+Y+GDVGSEWDQPP  MRP  SIPIKDYAIETP ++S +SS G+ F+EVKI
Sbjct: 126  GKWILHWGVSYVGDVGSEWDQPPSQMRPPDSIPIKDYAIETPMKKS-SSSGGDGFYEVKI 184

Query: 1348 DFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDG---NVVGAKKGLGIWQA 1178
            D + +S+IAAINFVLKD+E+G WYQHRGRDFKVPL+DY+Q DG   N+ GAK G  IW  
Sbjct: 185  DLSPNSAIAAINFVLKDDETGTWYQHRGRDFKVPLVDYVQGDGPDSNIAGAKWGFSIWP- 243

Query: 1177 GALGQISNMLHKPEAADSKGDD----IRESNLQKRPLQAFYEEHSVFKEIITDNSISVSA 1010
            GA GQ+S+++ K EA+ + G D     R+   + R L+ FY+E  + K++  DN+++VS 
Sbjct: 244  GAFGQLSSLILKGEASTTTGPDNSTNSRDPKQESRSLERFYDEQPIAKQVPVDNTVTVSV 303

Query: 1009 SHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQR 830
              C E AKN LH+ETDLPGDVVIHWGVC+D  K WEIPA PYPPETI FK+KALRT LQ 
Sbjct: 304  RKCPETAKNHLHVETDLPGDVVIHWGVCLDGAKKWEIPAAPYPPETIAFKDKALRTRLQP 363

Query: 829  KDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKG 650
            K+ G G WGSFT+++    F+FVLKLNE+TWLNC GNDFYIP +SS  +   +       
Sbjct: 364  KEGGDGCWGSFTVEEGLEGFLFVLKLNESTWLNCMGNDFYIPLSSSSLSARSTQSLSG-- 421

Query: 649  EEHLKEFGSPVSVDITSESNE-AVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESIL 473
                   G+ +   I  E+ E ++S +T GII EIRNLV DI           E QESIL
Sbjct: 422  -------GTQIPEKIAKENQEGSLSTYTSGIIKEIRNLVGDISSEKSQKTKSKEIQESIL 474

Query: 472  QEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKS 293
            +EIEKLAAEAYSIFRSS+PT  +  V E K+ +           G+EILCQGFNWESHKS
Sbjct: 475  REIEKLAAEAYSIFRSSVPTVIEEAVVEPKEEKAPVKICPGTGTGFEILCQGFNWESHKS 534

Query: 292  GKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSK 113
            G+WYMEL EKA ELSS+GFTVIWLPPPT+S+SPEGYMPRDLYNLNSRYGNIDQLK +V K
Sbjct: 535  GRWYMELKEKAEELSSIGFTVIWLPPPTESISPEGYMPRDLYNLNSRYGNIDQLKEVVKK 594

Query: 112  LHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
             HEVGIKVLGD VLNHRCA YKNQNGVWNI+GGRLNW
Sbjct: 595  FHEVGIKVLGDAVLNHRCAHYKNQNGVWNIYGGRLNW 631


>gb|OMP12226.1| hypothetical protein CCACVL1_00071 [Corchorus capsularis]
          Length = 626

 Score =  705 bits (1819), Expect = 0.0
 Identities = 368/637 (57%), Positives = 451/637 (70%), Gaps = 12/637 (1%)
 Frame = -3

Query: 1876 MSVVTTDRLFLHHHTCTTFKPVQKFQFISKKLQPFNPICPRPR----TRRANICS----R 1721
            M+ V  D L    +   +F+P  K +   K  +  N  C R R    +R A  CS    R
Sbjct: 1    MTTVAIDSLL--QNPALSFRP--KAKVFLKPSRSLN--CSRNRKLLFSRAACSCSFKPRR 54

Query: 1720 HFYIPKALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVG 1541
              ++ KA SS+   +++TS S +V + ETF +KR EK+EGKI I LD  K+++ W+LTVG
Sbjct: 55   KIHVVKA-SSTDTALIDTSSSDDVLYKETFPVKRIEKVEGKIFIMLDQSKDQQNWQLTVG 113

Query: 1540 CSLPGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFH 1361
            CSLPGKW+LHWGV+Y+GD GSEWDQPP DMRP GSIPI+DYAIETP ++    SEG+ FH
Sbjct: 114  CSLPGKWILHWGVSYVGDKGSEWDQPPKDMRPPGSIPIRDYAIETPLKKL---SEGDMFH 170

Query: 1360 EVKIDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQ 1181
            EVKIDFN S +IAAI+FVLKDEE+G WYQHRGRDFKVPL+DYL+DDGN+VGAK+G GIW 
Sbjct: 171  EVKIDFNPSCAIAAIHFVLKDEETGAWYQHRGRDFKVPLVDYLEDDGNMVGAKRGFGIWP 230

Query: 1180 AGALGQISNMLHKPEAADSKGD----DIRESNLQKRPLQAFYEEHSVFKEIITDNSISVS 1013
             GALGQ SNML   EA+         + ++S  +KR L+ FYEE  + KE+   N +SV+
Sbjct: 231  -GALGQFSNMLLNSEASHGSSQNGSSESKDSKREKRQLEGFYEEQPIVKEVPIGNLVSVT 289

Query: 1012 ASHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQ 833
               C E AK+IL +ETD+PGDVV+HWGVC D+ + WEIPA PYP ET  FK+KALRTLLQ
Sbjct: 290  VRKCSETAKDILCLETDIPGDVVVHWGVCRDDARTWEIPAAPYPLETTIFKSKALRTLLQ 349

Query: 832  RKDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSK 653
             K  G+GS   FTL++    F+FVLKL +NTW+N  GNDFYIP   +      ++ + S 
Sbjct: 350  PKGTGNGSTALFTLEEELVGFLFVLKLEDNTWVNFKGNDFYIPLAGATGVVSQNAKSDS- 408

Query: 652  GEEHLKEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESIL 473
                       VS  ++ E++    A+T+GIINEIRNLVS +           EAQESIL
Sbjct: 409  -----------VSDSVSEETSN--KAYTEGIINEIRNLVSGLNSEKSQKTKTKEAQESIL 455

Query: 472  QEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKS 293
            QEIEKLAAEAYSIFR+S+PT+++  V E +  +           G+EILCQGFNWES+KS
Sbjct: 456  QEIEKLAAEAYSIFRTSVPTFSEEAVLETEAPEPAVKISSGTGSGFEILCQGFNWESNKS 515

Query: 292  GKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSK 113
            G+WYMEL EKASE+SSLGFTVIWLPPPT+SVSPEGYMP DLYNLNSRYG ID+LK LV  
Sbjct: 516  GRWYMELKEKASEISSLGFTVIWLPPPTESVSPEGYMPSDLYNLNSRYGTIDELKELVKS 575

Query: 112  LHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            LHEVG+KVLGD VLNHRCAQYKNQNGVWNIFGGRLNW
Sbjct: 576  LHEVGLKVLGDAVLNHRCAQYKNQNGVWNIFGGRLNW 612


>ref|XP_022776933.1| alpha-amylase 3, chloroplastic-like isoform X3 [Durio zibethinus]
          Length = 750

 Score =  708 bits (1827), Expect = 0.0
 Identities = 358/601 (59%), Positives = 438/601 (72%), Gaps = 14/601 (2%)
 Frame = -3

Query: 1762 CPRPR----TRRANICS----RHFYIPKALSSSGAT--VVETSESSNVTFSETFHLKRPE 1613
            C R R    +R A  CS    R  ++ K+ SS  A     +TS S ++ + ETF +KR E
Sbjct: 33   CSRNRKLLFSRAARFCSSKPIRKIHVVKSSSSDTALFDTFDTSSSDDILYKETFPVKRIE 92

Query: 1612 KLEGKITIRLDNGKNEECWKLTVGCSLPGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSI 1433
            K+EGK+ IRLD  K++  W+L+VGCSLPGKW+LHWGV+Y+GD GSEWDQPP DMRP GS+
Sbjct: 93   KVEGKLVIRLDQSKDQHNWQLSVGCSLPGKWILHWGVSYLGDFGSEWDQPPKDMRPPGSV 152

Query: 1432 PIKDYAIETPFERSPTSSEGEAFHEVKIDFNTSSSIAAINFVLKDEESGYWYQHRGRDFK 1253
            PI+DYAIETP ++    SEG+ FHEVKIDFN SS+IAAI+FVLKDEE+G WYQHRGRDFK
Sbjct: 153  PIRDYAIETPLKKP---SEGDMFHEVKIDFNPSSAIAAIHFVLKDEETGAWYQHRGRDFK 209

Query: 1252 VPLIDYLQDDGNVVGAKKGLGIWQAGALGQISNMLHKPEAADSKG----DDIRESNLQKR 1085
            VPL+DYL+DDGN+VGAK+G GIW  GALGQ SN+L K EA+ + G    ++ ++S  + R
Sbjct: 210  VPLVDYLEDDGNMVGAKRGFGIWP-GALGQFSNVLLKAEASHANGPNSNNESKDSKNENR 268

Query: 1084 PLQAFYEEHSVFKEIITDNSISVSASHCLERAKNILHIETDLPGDVVIHWGVCIDEGKNW 905
             L+ FYEE  + KE+   N +S++     + AKN+L++ETD+PGDVV+HWGVC D+ K W
Sbjct: 269  RLEGFYEEQPIVKEVSIGNLVSIAVRKSPDTAKNVLYMETDIPGDVVVHWGVCRDDAKTW 328

Query: 904  EIPAEPYPPETIRFKNKALRTLLQRKDDGSGSWGSFTLDQRFSAFVFVLKLNENTWLNCN 725
            EIPA PYPPET  FKNKALRTLLQ K  G+GS   FTLD+    F+FVLKL++NTWL   
Sbjct: 329  EIPAAPYPPETAVFKNKALRTLLQPKATGNGSRALFTLDEELVGFLFVLKLDDNTWLKFK 388

Query: 724  GNDFYIPFTSSVATDEHSSLTHSKGEEHLKEFGSPVSVDITSESNEAVSAHTDGIINEIR 545
             NDFY+P + +++       + S  EE            I S+      A+TD IINEIR
Sbjct: 389  ENDFYVPLSGAISVPGQHGQSDSTSEE------------IPSK------AYTDDIINEIR 430

Query: 544  NLVSDIXXXXXXXXXXXEAQESILQEIEKLAAEAYSIFRSSMPTYTDTDVSEDKDLQXXX 365
            NLVS +           EAQESILQEIEKLAAEAYSIFRSS+PT+++  V E +  +   
Sbjct: 431  NLVSGLNSEKSQKTKTKEAQESILQEIEKLAAEAYSIFRSSVPTFSEETVLETEATKPAV 490

Query: 364  XXXXXXXXGYEILCQGFNWESHKSGKWYMELYEKASELSSLGFTVIWLPPPTDSVSPEGY 185
                    G+EILCQGFNWESHKSG+WYMEL EKASE+SSLGFTVIWLPPPT+SVSPEGY
Sbjct: 491  TISSGTGTGFEILCQGFNWESHKSGRWYMELKEKASEISSLGFTVIWLPPPTESVSPEGY 550

Query: 184  MPRDLYNLNSRYGNIDQLKSLVSKLHEVGIKVLGDVVLNHRCAQYKNQNGVWNIFGGRLN 5
            MP+DLYNLNSRYG ID+LK LV  LHEVG+KVLGDVVLNHRCAQ++NQNGVWNIFGGRLN
Sbjct: 551  MPKDLYNLNSRYGTIDELKELVKSLHEVGLKVLGDVVLNHRCAQFQNQNGVWNIFGGRLN 610

Query: 4    W 2
            W
Sbjct: 611  W 611


>ref|XP_016547075.1| PREDICTED: alpha-amylase 3, chloroplastic [Capsicum annuum]
          Length = 890

 Score =  712 bits (1838), Expect = 0.0
 Identities = 358/570 (62%), Positives = 429/570 (75%), Gaps = 2/570 (0%)
 Frame = -3

Query: 1705 KALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSLPG 1526
            +A+S+  A +  + E S+V F E+F LKRPEK EGKI IRLD G +EE W L+VGCSLPG
Sbjct: 60   RAISTDAAVIETSEEQSDVVFKESFSLKRPEKAEGKIAIRLDKGNDEENWHLSVGCSLPG 119

Query: 1525 KWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVKID 1346
            KW+LHWGV+Y GD GSEWDQPP +M PSGSI IKDYAIETP +       GEAF EVKID
Sbjct: 120  KWILHWGVHYAGDTGSEWDQPPREMWPSGSIAIKDYAIETPLK-------GEAFQEVKID 172

Query: 1345 FNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGALG 1166
             ++  SIAAINFVLKDEE+G WYQHRGRDFK+PL+D L DD N VG KKG GIW +G+LG
Sbjct: 173  ISSKWSIAAINFVLKDEETGVWYQHRGRDFKIPLVDCLDDDANTVGVKKGSGIW-SGSLG 231

Query: 1165 QISNMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCLER 992
            ++SN+L KPEAA SKG+      S+++ R L+ FYEEH++ KE + DN++SVS   C E 
Sbjct: 232  KLSNILLKPEAAPSKGESSSNDGSSVKSRRLEGFYEEHAIVKETLVDNTVSVSVKQCPET 291

Query: 991  AKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDGSG 812
            AKNIL+I+TDLPG+V++HWG+C  + K WE+PA PYP ET+ FKN ALRTLLQRK+ G+G
Sbjct: 292  AKNILYIDTDLPGNVILHWGICKGDTKIWELPAMPYPTETVVFKNNALRTLLQRKEGGNG 351

Query: 811  SWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHLKE 632
            S G FTLD   S F FV+KL+ENTWLNC G+DFYIP  S     +H S  +       +E
Sbjct: 352  SKGLFTLDGGPSGFAFVVKLDENTWLNCKGDDFYIPLPSG----QHQSKEN-------EE 400

Query: 631  FGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEKLA 452
              S   V+ + E ++A S +TD II EIRNLVSDI           E QE+ILQEIEKLA
Sbjct: 401  SNSSQIVNRSPEQSQAGSVYTDEIIKEIRNLVSDISSEKSRKTKTKETQETILQEIEKLA 460

Query: 451  AEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYMEL 272
            AEAYSIFRSS+PT ++  VSE + LQ           G+EILCQGFNWESHKSG+WY EL
Sbjct: 461  AEAYSIFRSSIPTISEV-VSEAEVLQPDVKVTSGTGTGFEILCQGFNWESHKSGRWYKEL 519

Query: 271  YEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVGIK 92
            ++KA+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG++D+LK  V K HEVGIK
Sbjct: 520  HDKAAELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSLDELKVTVKKFHEVGIK 579

Query: 91   VLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            VLGDVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 580  VLGDVVLNHRCASERNQNGIWNIFGGRLNW 609


>ref|XP_015075683.1| PREDICTED: alpha-amylase 3, chloroplastic [Solanum pennellii]
          Length = 892

 Score =  711 bits (1836), Expect = 0.0
 Identities = 353/572 (61%), Positives = 426/572 (74%), Gaps = 2/572 (0%)
 Frame = -3

Query: 1711 IPKALSSSGATVVETSESSNVTFSETFHLKRPEKLEGKITIRLDNGKNEECWKLTVGCSL 1532
            +P   SS+ A V+ETSE S+V F ETF LKRPE+ EGKI+IRLD GK+EE W LTVGCSL
Sbjct: 56   VPIRASSTDAVVIETSEQSDVVFKETFSLKRPERAEGKISIRLDKGKDEENWNLTVGCSL 115

Query: 1531 PGKWVLHWGVNYIGDVGSEWDQPPLDMRPSGSIPIKDYAIETPFERSPTSSEGEAFHEVK 1352
            PGKW+LHWGV+Y  D GSEWDQPP +MRPSGSI IKDYAIETP +       G+ F EVK
Sbjct: 116  PGKWILHWGVHYTDDTGSEWDQPPPEMRPSGSIAIKDYAIETPLQ-------GDTFQEVK 168

Query: 1351 IDFNTSSSIAAINFVLKDEESGYWYQHRGRDFKVPLIDYLQDDGNVVGAKKGLGIWQAGA 1172
            ID ++  SIAAINFVLKDEE+G WYQHRGRDFK+PL+D L DD N+VG KK   IW +G+
Sbjct: 169  IDISSKWSIAAINFVLKDEETGVWYQHRGRDFKIPLVDCLDDDANIVGVKKESNIW-SGS 227

Query: 1171 LGQISNMLHKPEAADSKGDDIRE--SNLQKRPLQAFYEEHSVFKEIITDNSISVSASHCL 998
            LG++SN+L  PEA+ SKG+      S+ + R L+ FYEEH++ KE + DN ++VS   C 
Sbjct: 228  LGKLSNILLNPEASPSKGESSSNEGSSAKNRRLEGFYEEHAIVKETLVDNIVNVSVKQCP 287

Query: 997  ERAKNILHIETDLPGDVVIHWGVCIDEGKNWEIPAEPYPPETIRFKNKALRTLLQRKDDG 818
            E AKNIL ++TDLPG+V++HWG+   + K WE+PA PYP ET+ FKNKALRTLLQRK+ G
Sbjct: 288  ETAKNILCVDTDLPGNVILHWGIYKGDAKIWELPAMPYPAETVVFKNKALRTLLQRKEGG 347

Query: 817  SGSWGSFTLDQRFSAFVFVLKLNENTWLNCNGNDFYIPFTSSVATDEHSSLTHSKGEEHL 638
            +GS G FTLD   + FVFV+KL+ENTWLNC G+DFY+P +S           H +  +  
Sbjct: 348  NGSSGLFTLDVGLAGFVFVVKLDENTWLNCKGDDFYVPLSSGTL--------HLEESKQS 399

Query: 637  KEFGSPVSVDITSESNEAVSAHTDGIINEIRNLVSDIXXXXXXXXXXXEAQESILQEIEK 458
            +E  S   V+ T E ++  S +TD II EIR+LVSDI           E QE+ILQEIEK
Sbjct: 400  EESNSSQIVNRTPEESQIGSVYTDEIIKEIRSLVSDISSEKSRKTKNRETQETILQEIEK 459

Query: 457  LAAEAYSIFRSSMPTYTDTDVSEDKDLQXXXXXXXXXXXGYEILCQGFNWESHKSGKWYM 278
            LAAEAY IFRSS+PT  +T VSE + +Q           G+EILCQGFNWESHKSG+WY 
Sbjct: 460  LAAEAYGIFRSSIPTILETVVSESELVQPDVKVTSGTGTGFEILCQGFNWESHKSGRWYK 519

Query: 277  ELYEKASELSSLGFTVIWLPPPTDSVSPEGYMPRDLYNLNSRYGNIDQLKSLVSKLHEVG 98
            EL++KA+ELSSLGF+VIWLPPPTDSVSPEGYMPRDLYNLNSRYG+ D+LK  V K HEVG
Sbjct: 520  ELHDKAAELSSLGFSVIWLPPPTDSVSPEGYMPRDLYNLNSRYGSFDELKVTVKKFHEVG 579

Query: 97   IKVLGDVVLNHRCAQYKNQNGVWNIFGGRLNW 2
            IKVLGDVVLNHRCA  +NQNG+WNIFGGRLNW
Sbjct: 580  IKVLGDVVLNHRCASERNQNGIWNIFGGRLNW 611