BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00016441
         (478 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KZV26181.1| hypothetical protein F511_06348 [Dorcoceras hygro...    72   2e-11
ref|XP_016679133.1| PREDICTED: uncharacterized protein LOC107898...    45   2e-09
ref|XP_022136882.1| dr1-associated corepressor homolog isoform X...    64   6e-09
emb|CAN62182.1| hypothetical protein VITISV_044400 [Vitis vinifera]    45   6e-09
ref|XP_016452669.1| PREDICTED: uncharacterized protein LOC107777...    53   1e-08
ref|XP_015382182.1| PREDICTED: uncharacterized protein LOC107175...    63   2e-08
emb|CAN83392.1| hypothetical protein VITISV_041406 [Vitis vinifera]    63   2e-08
ref|XP_010050843.1| PREDICTED: uncharacterized protein LOC104439...    62   4e-08
ref|XP_022152240.1| uncharacterized protein LOC111020007 [Momord...    60   4e-08
ref|XP_018808216.1| PREDICTED: uncharacterized protein LOC108981...    62   5e-08
gb|OIT00609.1| hypothetical protein A4A49_60155, partial [Nicoti...    60   5e-08
emb|CAN71138.1| hypothetical protein VITISV_008660 [Vitis vinifera]    52   6e-08
ref|XP_008341689.1| PREDICTED: uncharacterized protein LOC103404...    61   7e-08
ref|XP_017648096.1| PREDICTED: uncharacterized protein LOC108488...    47   8e-08
ref|XP_018825237.1| PREDICTED: uncharacterized protein LOC108994...    60   8e-08
ref|XP_008246085.1| PREDICTED: flocculation protein FLO11-like [...    60   9e-08
emb|CAN60188.1| hypothetical protein VITISV_002117 [Vitis vinifera]    44   1e-07
emb|CAN68623.1| hypothetical protein VITISV_010681 [Vitis vinifera]    51   1e-07
ref|XP_018818564.1| PREDICTED: uncharacterized protein LOC108989...    48   1e-07
ref|XP_015383711.1| PREDICTED: uncharacterized protein LOC107176...    60   1e-07

>gb|KZV26181.1| hypothetical protein F511_06348 [Dorcoceras hygrometricum]
          Length = 706

 Score = 71.6 bits (174), Expect = 2e-11
 Identities = 35/65 (53%), Positives = 47/65 (72%), Gaps = 2/65 (3%)
 Frame = -2

Query: 384 QFNPHS--FNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGN 211
           QFN  S  + PSA  +T++ES S+  WYPDS AS+HV  D+GNL+++SE+ G  KV +GN
Sbjct: 246 QFNRSSPSYPPSAFASTKSESASEEWWYPDSGASHHVTNDLGNLSVSSEYTGGSKVQVGN 305

Query: 210 GAGSS 196
           GAG S
Sbjct: 306 GAGLS 310


>ref|XP_016679133.1| PREDICTED: uncharacterized protein LOC107898098 [Gossypium
           hirsutum]
          Length = 704

 Score = 45.1 bits (105), Expect(2) = 2e-09
 Identities = 22/53 (41%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
 Frame = -2

Query: 363 NPSAQLTTRNESDS--DCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGN 211
           N S+Q+  R  + S  D  WYPDS A+NH+  ++ NL  AS + G   V +GN
Sbjct: 253 NSSSQVPLRASTSSQPDSIWYPDSGATNHITPEVSNLTTASLYSGANHVTMGN 305



 Score = 44.3 bits (103), Expect(2) = 2e-09
 Identities = 25/68 (36%), Positives = 38/68 (55%)
 Frame = -3

Query: 206 QDQATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQGRRHSLSANAIHSKKVPSLA 27
           +D  T  TLL+G ++ GLY FQL  +     AP S S   G     S + ++S ++ S  
Sbjct: 335 KDIQTWMTLLEGHMHKGLYRFQLFKS-----APKSVSTAAGYSSKSSPSLLNSAQLSSST 389

Query: 26  IWHSRLGH 3
           +WH++LGH
Sbjct: 390 LWHNKLGH 397


>ref|XP_022136882.1| dr1-associated corepressor homolog isoform X1 [Momordica charantia]
          Length = 326

 Score = 63.9 bits (154), Expect = 6e-09
 Identities = 37/104 (35%), Positives = 53/104 (50%), Gaps = 9/104 (8%)
 Frame = -2

Query: 453 QSHLLNQNFGQPTPSNN----VVGNYYQ-----FNPHSFNPSAQLTTRNESDSDCTWYPD 301
           QSH+ ++  G    S N      GN  Q     F P+S +  A    + + + D  WYPD
Sbjct: 221 QSHMQHKFSGGSNSSINSNMTAFGNQQQAFTNGFQPNSNSHMAAFLAQQDFNRDTNWYPD 280

Query: 300 SAASNHVAYDMGNLNIASEFQGDEKVHIGNGAGSSYRGHTSSRD 169
           S A+NHV  +  NL  ++E+ GD +V IGNG G S   H + R+
Sbjct: 281 SGATNHVTSNFNNLATSTEYTGDNQVRIGNGTGPSNWPHFTPRE 324


>emb|CAN62182.1| hypothetical protein VITISV_044400 [Vitis vinifera]
          Length = 920

 Score = 45.4 bits (106), Expect(2) = 6e-09
 Identities = 21/56 (37%), Positives = 34/56 (60%)
 Frame = -2

Query: 378 NPHSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGN 211
           N +  N  A + + + S +D +WY DS AS+H+  ++GNL   S + G ++V IGN
Sbjct: 173 NGNQNNILAMVASFSNSSADESWYLDSGASHHLTQNLGNLTSTSPYTGTDRVTIGN 228



 Score = 42.4 bits (98), Expect(2) = 6e-09
 Identities = 25/68 (36%), Positives = 35/68 (51%)
 Frame = -3

Query: 206 QDQATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQGRRHSLSANAIHSKKVPSLA 27
           +D+ T   L QG + NGLY F + +N K    P S   N    HS  ++ I +K      
Sbjct: 253 KDRHTKMVLAQGKLENGLYKFPVFSNKK----PYSSVNNASAFHSHFSSTIENK----AE 304

Query: 26  IWHSRLGH 3
           +WH+RLGH
Sbjct: 305 LWHNRLGH 312


>ref|XP_016452669.1| PREDICTED: uncharacterized protein LOC107777190 [Nicotiana tabacum]
          Length = 222

 Score = 52.8 bits (125), Expect(2) = 1e-08
 Identities = 28/82 (34%), Positives = 44/82 (53%)
 Frame = -2

Query: 459 FTQSHLLNQNFGQPTPSNNVVGNYYQFNPHSFNPSAQLTTRNESDSDCTWYPDSAASNHV 280
           FTQ +L  ++ G   PS N   N   ++ + +  S     +  +  D  WYPDS  +NH+
Sbjct: 79  FTQVNLTTKSSGH-NPSRNKHENKRNYSTNQYQ-SGTYVAQPLTVVDPLWYPDSGTTNHL 136

Query: 279 AYDMGNLNIASEFQGDEKVHIG 214
             D+ N+NI  E+ G E++HIG
Sbjct: 137 TNDLNNMNIMGEYSGFEQMHIG 158



 Score = 33.9 bits (76), Expect(2) = 1e-08
 Identities = 24/70 (34%), Positives = 31/70 (44%)
 Frame = -3

Query: 212 MGQDQATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQGRRHSLSANAIHSKKVPS 33
           M Q+  T   LLQG + +GLY+              S  F   R  S     + SK   S
Sbjct: 159 MVQELTTRKVLLQGRLEHGLYTL------------CSARFQSNRGSSQHQALLVSKS--S 204

Query: 32  LAIWHSRLGH 3
           + +WHSRLGH
Sbjct: 205 VDLWHSRLGH 214


>ref|XP_015382182.1| PREDICTED: uncharacterized protein LOC107175291 [Citrus sinensis]
          Length = 433

 Score = 62.8 bits (151), Expect = 2e-08
 Identities = 35/88 (39%), Positives = 48/88 (54%), Gaps = 9/88 (10%)
 Frame = -2

Query: 432 NFGQPTPSNNVVGNYYQ-FNP--HSFNPSAQLTTRN------ESDSDCTWYPDSAASNHV 280
           NF  PT  +N    +Y  FNP  HS  PSA+  T        E   D  WY DS A++H+
Sbjct: 240 NFAAPTVEDNYAAPFYSGFNPMYHSSYPSAEFNTPEAYLTNYEGPVDDGWYLDSGATHHL 299

Query: 279 AYDMGNLNIASEFQGDEKVHIGNGAGSS 196
             +M N+N+  EF+G +++ IGNG G S
Sbjct: 300 TNNMANMNVREEFKGSDQLVIGNGQGLS 327


>emb|CAN83392.1| hypothetical protein VITISV_041406 [Vitis vinifera]
          Length = 1262

 Score = 62.8 bits (151), Expect = 2e-08
 Identities = 23/44 (52%), Positives = 33/44 (75%)
 Frame = -2

Query: 333 ESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGNGAG 202
           E   +C W+PDS A+NHV +D+GNLN  +E+ G+ K+H+GNG G
Sbjct: 260 EDLQNCCWFPDSGATNHVTHDLGNLNSGAEYNGNSKIHMGNGTG 303


>ref|XP_010050843.1| PREDICTED: uncharacterized protein LOC104439476 [Eucalyptus
           grandis]
          Length = 381

 Score = 61.6 bits (148), Expect = 4e-08
 Identities = 29/68 (42%), Positives = 42/68 (61%), Gaps = 4/68 (5%)
 Frame = -2

Query: 390 YYQFNP----HSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKV 223
           Y +FNP    +S +PSA   +      D  WYPDS A++H+  DM NLNI S++ G +++
Sbjct: 303 YQRFNPGYKANSGSPSAMYASSIGESFDPYWYPDSGATHHLTTDMENLNIRSDYSGTDQI 362

Query: 222 HIGNGAGS 199
           H  NG+GS
Sbjct: 363 HTANGSGS 370


>ref|XP_022152240.1| uncharacterized protein LOC111020007 [Momordica charantia]
          Length = 168

 Score = 59.7 bits (143), Expect = 4e-08
 Identities = 26/50 (52%), Positives = 36/50 (72%)
 Frame = -2

Query: 348 LTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGNGAGS 199
           LT +++ + +  W+PDS  SNHV  D+ NL I++E+ GD KV IGNGAGS
Sbjct: 118 LTLQHDLNKENQWFPDSGTSNHVISDLANLGISTEYLGDNKVLIGNGAGS 167


>ref|XP_018808216.1| PREDICTED: uncharacterized protein LOC108981481 [Juglans regia]
          Length = 522

 Score = 61.6 bits (148), Expect = 5e-08
 Identities = 27/63 (42%), Positives = 45/63 (71%), Gaps = 2/63 (3%)
 Frame = -2

Query: 387 YQFNP-HSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASE-FQGDEKVHIG 214
           YQ++P  SF+ +   +    ++SD TWYPDSAA++H+  D+GNLN++SE + G E + +G
Sbjct: 323 YQYDPPQSFSANYTTSHNQTTNSDSTWYPDSAATHHITSDLGNLNLSSEPYHGPEAIRVG 382

Query: 213 NGA 205
           +G+
Sbjct: 383 DGS 385


>gb|OIT00609.1| hypothetical protein A4A49_60155, partial [Nicotiana attenuata]
          Length = 226

 Score = 60.5 bits (145), Expect = 5e-08
 Identities = 25/70 (35%), Positives = 42/70 (60%)
 Frame = -2

Query: 411 SNNVVGNYYQFNPHSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGD 232
           +N+     YQ+  H+ NPSA L+ +        WYPD+ A++H+A D+ +     E++G 
Sbjct: 154 TNHQAATCYQWYNHTINPSAHLSHQEPILLTQNWYPDTGATHHIAPDLSSFTYVEEYKGP 213

Query: 231 EKVHIGNGAG 202
           +++HIGNG G
Sbjct: 214 DQLHIGNGQG 223


>emb|CAN71138.1| hypothetical protein VITISV_008660 [Vitis vinifera]
          Length = 1138

 Score = 51.6 bits (122), Expect(2) = 6e-08
 Identities = 30/84 (35%), Positives = 41/84 (48%)
 Frame = -2

Query: 444 LLNQNFGQPTPSNNVVGNYYQFNPHSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMG 265
           L+ QN     PS      YY F+P        +   +E  +D  WYPD  ASNHV ++  
Sbjct: 321 LVPQNLSGRNPSLRA---YYSFSPQVNG----VIPTSEVFNDDNWYPDPGASNHVTHNPA 373

Query: 264 NLNIASEFQGDEKVHIGNGAGSSY 193
           NL  + EF    +VH+GNG  S +
Sbjct: 374 NLMKSVEFARQNQVHVGNGTVSKF 397



 Score = 32.7 bits (73), Expect(2) = 6e-08
 Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
 Frame = -3

Query: 200 QATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQGRRHSLSANAIHSKKVPS---- 33
           Q T A L+ G V +GLY+F  S  +      + P+ +  +  S+ A++  SK   +    
Sbjct: 417 QVTQAVLMVGKVRDGLYAFDSSHLA------LRPTQSLSKSPSVVASSFSSKVCTASLSS 470

Query: 32  -LAIWHSRLGH 3
              +WH RLGH
Sbjct: 471 TFDLWHRRLGH 481


>ref|XP_008341689.1| PREDICTED: uncharacterized protein LOC103404538 [Malus domestica]
          Length = 336

 Score = 60.8 bits (146), Expect = 7e-08
 Identities = 33/71 (46%), Positives = 44/71 (61%)
 Frame = -2

Query: 384 QFNPHSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGNGA 205
           Q  PHS  P A  TT   S S  TW  DS AS+HV  ++  L+ AS + G +++ IGNG+
Sbjct: 265 QSGPHS-PPVANPTTHGRSTSSPTWLLDSGASHHVTSNVNTLSGASIYDGPDEIIIGNGS 323

Query: 204 GSSYRGHTSSR 172
           GS +RG +SSR
Sbjct: 324 GSEHRGGSSSR 334


>ref|XP_017648096.1| PREDICTED: uncharacterized protein LOC108488325 [Gossypium
           arboreum]
          Length = 860

 Score = 47.0 bits (110), Expect(2) = 8e-08
 Identities = 29/75 (38%), Positives = 39/75 (52%), Gaps = 4/75 (5%)
 Frame = -2

Query: 420 PTPS--NNVVGNYYQFNP-HSFNPSAQLTTRN-ESDSDCTWYPDSAASNHVAYDMGNLNI 253
           PTPS  N  VGN  Q N   S  P + +     E+  D  WYPDS A++H+ +   NL  
Sbjct: 128 PTPSWTNPFVGNPLQVNALTSAAPQSNVFLATAETIGDNAWYPDSGATHHLTHSASNLGD 187

Query: 252 ASEFQGDEKVHIGNG 208
           +    G  KV++GNG
Sbjct: 188 SFSHSGPGKVYVGNG 202



 Score = 37.0 bits (84), Expect(2) = 8e-08
 Identities = 24/67 (35%), Positives = 34/67 (50%)
 Frame = -3

Query: 203 DQATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQGRRHSLSANAIHSKKVPSLAI 24
           D  T   LL G+V +GLY   L   +++  A  +        H  +AN     +VP L++
Sbjct: 207 DLKTNEVLLHGSVRHGLYKLHLKAAAENGQAASTA-------HYFTANT----RVP-LSV 254

Query: 23  WHSRLGH 3
           WHSRLGH
Sbjct: 255 WHSRLGH 261


>ref|XP_018825237.1| PREDICTED: uncharacterized protein LOC108994468 [Juglans regia]
          Length = 244

 Score = 60.1 bits (144), Expect = 8e-08
 Identities = 27/63 (42%), Positives = 45/63 (71%), Gaps = 1/63 (1%)
 Frame = -2

Query: 387 YQFN-PHSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGN 211
           YQ+  PHSF+ +    T   + S+ TWYPD+AA++H+ +D+ NLN++SE  G E++ +G+
Sbjct: 166 YQYEAPHSFSTNF---TSPSAFSNATWYPDTAATHHITHDLTNLNLSSEPYGGEQIRVGD 222

Query: 210 GAG 202
           G+G
Sbjct: 223 GSG 225


>ref|XP_008246085.1| PREDICTED: flocculation protein FLO11-like [Prunus mume]
          Length = 260

 Score = 60.1 bits (144), Expect = 9e-08
 Identities = 30/79 (37%), Positives = 45/79 (56%), Gaps = 1/79 (1%)
 Frame = -2

Query: 441 LNQNFGQPTPSNNVVGNYYQFNPHSFNPSAQLTT-RNESDSDCTWYPDSAASNHVAYDMG 265
           LN  F  PTP +++  N        +NPSA  T+    S S  TWY DS A+NH+  ++ 
Sbjct: 161 LNMAFQGPTPPSSLTQNTTPSILGPYNPSASFTSFAPPSTSTTTWYADSGATNHITNNLA 220

Query: 264 NLNIASEFQGDEKVHIGNG 208
           NL++ S +QG+E + + NG
Sbjct: 221 NLSLPSPYQGNESITVANG 239


>emb|CAN60188.1| hypothetical protein VITISV_002117 [Vitis vinifera]
          Length = 1079

 Score = 43.9 bits (102), Expect(2) = 1e-07
 Identities = 19/41 (46%), Positives = 26/41 (63%)
 Frame = -2

Query: 333 ESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGN 211
           E+  D  WYPDS ASNHV  +  NL   + + G+E+V +GN
Sbjct: 339 ENICDTNWYPDSGASNHVTTNANNLVEHTPYYGNEQVRVGN 379



 Score = 39.7 bits (91), Expect(2) = 1e-07
 Identities = 27/82 (32%), Positives = 38/82 (46%), Gaps = 12/82 (14%)
 Frame = -3

Query: 212 MGQDQATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQ---GRRHSLSAN---AIH 51
           +G DQ +   +L+G +  GLY+F  S       AP   S +Q     +  L AN   +IH
Sbjct: 377 VGNDQVSKIVVLEGKLKGGLYAFDSSQIQLKKSAPSQVSLSQSVTSHQSGLRANCQVSIH 436

Query: 50  SKKVPS------LAIWHSRLGH 3
              + S      + IWH RLGH
Sbjct: 437 VSDLQSQNGSSTVGIWHDRLGH 458


>emb|CAN68623.1| hypothetical protein VITISV_010681 [Vitis vinifera]
          Length = 846

 Score = 51.2 bits (121), Expect(2) = 1e-07
 Identities = 25/61 (40%), Positives = 35/61 (57%), Gaps = 1/61 (1%)
 Frame = -2

Query: 372 HSFNPSAQ-LTTRNESDSDCTWYPDSAASNHVAYDMGNLNIASEFQGDEKVHIGNGAGSS 196
           +SF+P    +   +E  SD  WYPDS  SNHV     N+  ++EF G  +VH+GNG  S 
Sbjct: 80  YSFSPQVNGVIPTSEVFSDDNWYPDSRVSNHVTPIPANMMKSAEFAGQNQVHVGNGTVSK 139

Query: 195 Y 193
           +
Sbjct: 140 F 140



 Score = 32.3 bits (72), Expect(2) = 1e-07
 Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
 Frame = -3

Query: 206 QDQATGATLLQGTVNNGLYSFQLS------TNSKSI-PAPVSPSFNQGRRHSLSANAIHS 48
           +DQ     L+ G + +GLY+F  S      T S S+ P+ V+ SF        S+    +
Sbjct: 158 KDQVIQVVLMVGKIRDGLYAFDSSHLALRPTQSLSMSPSVVASSF--------SSKVCTA 209

Query: 47  KKVPSLAIWHSRLGH 3
               +  +WH RLGH
Sbjct: 210 SLSSTFDLWHRRLGH 224


>ref|XP_018818564.1| PREDICTED: uncharacterized protein LOC108989421 [Juglans regia]
          Length = 226

 Score = 48.1 bits (113), Expect(2) = 1e-07
 Identities = 28/85 (32%), Positives = 46/85 (54%), Gaps = 8/85 (9%)
 Frame = -2

Query: 414 PSNNVVGNYYQFN-------PHSFNPSAQLTTRNESDSDCTWYPDSAASNHVAYDMGNLN 256
           P +  +  Y++FN       P SF  +A  TT +   S   W+PD+ A++H  YD  NLN
Sbjct: 18  PGHTAIRCYHRFNQAYQSPPPSSF--TANFTTTSPVPSSNAWFPDTIATSHFTYDFSNLN 75

Query: 255 I-ASEFQGDEKVHIGNGAGSSYRGH 184
           + A+ +Q  ++V IG+   +S  G+
Sbjct: 76  LDAAVYQDPDQVSIGSANLNSSSGN 100



 Score = 35.4 bits (80), Expect(2) = 1e-07
 Identities = 23/68 (33%), Positives = 28/68 (41%)
 Frame = -3

Query: 206 QDQATGATLLQGTVNNGLYSFQLSTNSKSIPAPVSPSFNQGRRHSLSANAIHSKKVPSLA 27
           +D  T  TLLQG V NGLY   +  +     + VSP      R              S  
Sbjct: 140 KDSQTRETLLQGHVRNGLYELHVDVSVSPPSSNVSPQACLSER-------------TSPQ 186

Query: 26  IWHSRLGH 3
           +WH RLGH
Sbjct: 187 MWHFRLGH 194


>ref|XP_015383711.1| PREDICTED: uncharacterized protein LOC107176156 [Citrus sinensis]
          Length = 509

 Score = 60.5 bits (145), Expect = 1e-07
 Identities = 33/88 (37%), Positives = 49/88 (55%), Gaps = 9/88 (10%)
 Frame = -2

Query: 432 NFGQPTPSNNVVGNYYQ-FNP--HSFNPSAQLT------TRNESDSDCTWYPDSAASNHV 280
           N+  PT  +N    +Y  FNP  HS  PSA+        T  E  +D  WY DS A++H+
Sbjct: 234 NYAAPTVEDNYAAPFYSGFNPMYHSSYPSAEFNAPEAYLTNYEGPADDGWYLDSGATHHL 293

Query: 279 AYDMGNLNIASEFQGDEKVHIGNGAGSS 196
             +M N+N+  EF+G +++ IG+G G S
Sbjct: 294 TNNMANMNVREEFKGSDQLVIGDGHGLS 321