BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Rehmannia32_contig00015330
         (648 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011069755.1| isoflavone 3'-hydroxylase-like [Sesamum indi...   265   5e-83
gb|KZV56200.1| hypothetical protein F511_13804 [Dorcoceras hygro...   251   1e-73
emb|CDP01234.1| unnamed protein product [Coffea canephora]            238   2e-72
gb|AJD25198.1| cytochrome P450 CYP81Q42 [Salvia miltiorrhiza]         228   5e-69
gb|PIM99210.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   228   8e-69
gb|KZN00085.1| hypothetical protein DCAR_008839 [Daucus carota s...   217   7e-67
gb|PIN02408.1| Cytochrome P450 CYP2 subfamily [Handroanthus impe...   222   2e-66
ref|XP_024166813.1| cytochrome P450 81E8-like [Rosa chinensis] >...   207   6e-61
gb|PON48894.1| Cytochrome P450, E-class, group I [Parasponia and...   201   2e-60
ref|XP_007227313.2| cytochrome P450 81D11 [Prunus persica] >gi|1...   205   6e-60
gb|PON31981.1| Cytochrome P450, E-class, group I, partial [Trema...   198   2e-59
ref|XP_021820295.1| isoflavone 3'-hydroxylase-like [Prunus avium]     204   2e-59
gb|POF08330.1| cytochrome p450 81e8 [Quercus suber]                   195   3e-59
ref|XP_017605887.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   202   6e-59
ref|XP_004290938.1| PREDICTED: isoflavone 3'-hydroxylase-like [F...   203   7e-59
ref|XP_002510139.1| PREDICTED: isoflavone 2'-hydroxylase [Ricinu...   202   7e-59
ref|XP_012068273.1| isoflavone 2'-hydroxylase [Jatropha curcas] ...   202   8e-59
ref|XP_022772222.1| isoflavone 2'-hydroxylase-like [Durio zibeth...   201   3e-58
gb|POE89360.1| isoflavone 2'-hydroxylase [Quercus suber]              196   6e-58
ref|XP_016749332.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   199   9e-58

>ref|XP_011069755.1| isoflavone 3'-hydroxylase-like [Sesamum indicum]
          Length = 509

 Score =  265 bits (676), Expect = 5e-83
 Identities = 135/206 (65%), Positives = 163/206 (79%)
 Frame = +1

Query: 31  MEITLWYIILSAIFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSE 210
           ME+TL+  IL  +F+L L++FL   Q              IGHLHLVKEPL++ LQALS 
Sbjct: 1   MELTLYCTIL-LVFLLTLRHFLVKQQRNNKPPSPPALPL-IGHLHLVKEPLNQTLQALSA 58

Query: 211 KYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYG 390
           KYG+IL L+LG RKVL+V SP+AVEECFT+ND++FA RP TL+TKHFSY++TTIS+APYG
Sbjct: 59  KYGDILSLRLGVRKVLVVTSPAAVEECFTKNDVIFANRPSTLSTKHFSYNYTTISIAPYG 118

Query: 391 DHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEI 570
           DHWRNLRRLAALEIFS ARI+MF   R +EL +LL+EL R+++  GGSTKVDL SKF E+
Sbjct: 119 DHWRNLRRLAALEIFSPARIAMFTATRRRELSLLLNELLRDAEAGGGSTKVDLKSKFIEL 178

Query: 571 VFNVLSMTIAGKRYYGENVVDARKVR 648
            FNVLSMTIAGKRYYGENVVDA + R
Sbjct: 179 AFNVLSMTIAGKRYYGENVVDAEEAR 204


>gb|KZV56200.1| hypothetical protein F511_13804 [Dorcoceras hygrometricum]
          Length = 1082

 Score =  251 bits (640), Expect = 1e-73
 Identities = 128/206 (62%), Positives = 155/206 (75%)
 Frame = +1

Query: 31  MEITLWYIILSAIFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSE 210
           ME T  YIIL  +  L++       +              IGHLHL+KEP++R L+ALS 
Sbjct: 1   MEQTFCYIILLILSSLLVSTHFVVKKRRRNGPPSPFGLPVIGHLHLIKEPMNRTLEALSG 60

Query: 211 KYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYG 390
           K+G+ILQLQLG RKVL++ SPSAVEECFT+ND++FA RP TL+TKHFSY+ TTIS+APYG
Sbjct: 61  KHGDILQLQLGVRKVLVLTSPSAVEECFTKNDMIFANRPSTLSTKHFSYNNTTISIAPYG 120

Query: 391 DHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEI 570
           +HWRNLRR+AA EIFS ARI+MF   R +EL +LL EL R+SK+ GGST VDL SKF E+
Sbjct: 121 EHWRNLRRIAAHEIFSPARIAMFANTRKRELDLLLGELLRDSKLGGGSTVVDLKSKFIEL 180

Query: 571 VFNVLSMTIAGKRYYGENVVDARKVR 648
            FNVLSMTIAGKRYYGENVVDA + R
Sbjct: 181 SFNVLSMTIAGKRYYGENVVDAEEAR 206


>emb|CDP01234.1| unnamed protein product [Coffea canephora]
          Length = 514

 Score =  238 bits (606), Expect = 2e-72
 Identities = 120/200 (60%), Positives = 146/200 (73%)
 Frame = +1

Query: 49  YIILSAIFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSEKYGNIL 228
           ++ +S IFIL L  F+                  IGHLHL+KEPL++ LQ+L  KYG+IL
Sbjct: 10  FLAISIIFILNL--FIRKRALQKKQPPSPPSLPLIGHLHLLKEPLNQTLQSLCSKYGDIL 67

Query: 229 QLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYGDHWRNL 408
            L+LG RKVL+V SPSA EECFT+NDI+FA RP T+++KHFSY++TTISVAPYGDHWRNL
Sbjct: 68  LLRLGVRKVLIVTSPSAAEECFTKNDIIFANRPKTMSSKHFSYNYTTISVAPYGDHWRNL 127

Query: 409 RRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEIVFNVLS 588
           RRLAALEIFS AR++ F   R KELM+LLSEL +   + GGS KVDL S   EI FN+LS
Sbjct: 128 RRLAALEIFSPARMASFASTRRKELMLLLSELMQKCSVGGGSAKVDLTSMSAEITFNILS 187

Query: 589 MTIAGKRYYGENVVDARKVR 648
           MT+AGKRYYGEN  D  + R
Sbjct: 188 MTLAGKRYYGENAADDEEAR 207


>gb|AJD25198.1| cytochrome P450 CYP81Q42 [Salvia miltiorrhiza]
          Length = 493

 Score =  228 bits (581), Expect = 5e-69
 Identities = 119/205 (58%), Positives = 148/205 (72%)
 Frame = +1

Query: 34  EITLWYIILSAIFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSEK 213
           E TL++ IL  + +L+L       +              IGHLHL+K+P+ + LQ+LS K
Sbjct: 3   EFTLFFTILVILVLLLL--IQHKKKQRQNRPPSPPSLPLIGHLHLLKDPMTQTLQSLSAK 60

Query: 214 YGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYGD 393
           YG++L L+ G R VL++ SP+AV+ECFT NDIVFA RP TL+TKHFSY+ TTIS+APYGD
Sbjct: 61  YGDVLSLKFGVRDVLVITSPTAVDECFTTNDIVFANRPSTLSTKHFSYNNTTISIAPYGD 120

Query: 394 HWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEIV 573
           HWRNLRRLAALEIFS ARI+ F   R  ELM+L+ +L RNS   GG++ VDL SKF E+ 
Sbjct: 121 HWRNLRRLAALEIFSPARIAAFTATRRTELMLLMRDLARNS--GGGTSPVDLKSKFIELA 178

Query: 574 FNVLSMTIAGKRYYGENVVDARKVR 648
           FNVLSMTIAGKRYYGENV DA + R
Sbjct: 179 FNVLSMTIAGKRYYGENVGDAEEAR 203


>gb|PIM99210.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 498

 Score =  228 bits (580), Expect = 8e-69
 Identities = 124/205 (60%), Positives = 150/205 (73%), Gaps = 1/205 (0%)
 Frame = +1

Query: 37  ITLWYIILSAI-FILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSEK 213
           +TL  IIL+ + F+LILK+ LA  +              IGHL+L+KEPL++ LQALS K
Sbjct: 4   VTLSCIILAPLLFLLILKHHLAKKKTYNHPPSPPSLPI-IGHLYLIKEPLNQTLQALSAK 62

Query: 214 YGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYGD 393
              I       RKVLLV SP+AV ECFT+NDIVFA RP TL+TKHFSY++TTIS+APYGD
Sbjct: 63  SAAI-------RKVLLVTSPAAVNECFTKNDIVFANRPSTLSTKHFSYNYTTISIAPYGD 115

Query: 394 HWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEIV 573
           HWRNLR LAALEIFS +RI+ F  +R +ELM+LL+E  R+SK  GG T VDL SKF E+ 
Sbjct: 116 HWRNLRCLAALEIFSPSRITTFTNIRRRELMLLLNEFLRDSKNGGGKTMVDLKSKFIELA 175

Query: 574 FNVLSMTIAGKRYYGENVVDARKVR 648
           F+VLSMTIAGKRYYGENV DA + R
Sbjct: 176 FDVLSMTIAGKRYYGENVEDAEEAR 200


>gb|KZN00085.1| hypothetical protein DCAR_008839 [Daucus carota subsp. sativus]
          Length = 316

 Score =  217 bits (553), Expect = 7e-67
 Identities = 103/166 (62%), Positives = 131/166 (78%)
 Frame = +1

Query: 151 IGHLHLVKEPLHRNLQALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPF 330
           +GHLHL+KEPLH+ L+ LS KYG+IL L+ G +KVL++ SPSA+EECFTRND VFA RP 
Sbjct: 39  LGHLHLIKEPLHQTLETLSSKYGDILLLRFGLKKVLVICSPSAIEECFTRNDTVFANRPV 98

Query: 331 TLATKHFSYDFTTISVAPYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFR 510
           ++AT H SYDFTT++VAPYGD WRNLRRLAALEIFS  RI+ F  +R  E+M+L+++L +
Sbjct: 99  SMATIHLSYDFTTMTVAPYGDLWRNLRRLAALEIFSPNRIAFFTDIRDNEIMLLINQLVK 158

Query: 511 NSKISGGSTKVDLNSKFTEIVFNVLSMTIAGKRYYGENVVDARKVR 648
             K  GG  KV+L + F E+ FN+LSMTI G+RYYGENV DA + R
Sbjct: 159 --KCQGGGEKVELKTTFNELAFNILSMTIGGRRYYGENVEDAEEAR 202


>gb|PIN02408.1| Cytochrome P450 CYP2 subfamily [Handroanthus impetiginosus]
          Length = 505

 Score =  222 bits (565), Expect = 2e-66
 Identities = 118/205 (57%), Positives = 145/205 (70%), Gaps = 1/205 (0%)
 Frame = +1

Query: 37  ITLWYIILSAI-FILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSEK 213
           +TL  IIL+ + F+LILK+ LA  +              IGHL+L+KE    N  +   K
Sbjct: 4   VTLSCIILAPLLFLLILKHHLAKKKTYNHPPSPPSLPI-IGHLYLIKETTKPNSPSSLRK 62

Query: 214 YGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYGD 393
            G +L L+   RKVLLV SP+AV ECFT+NDIVFA  P TL+TKHFSY++TTIS+APYGD
Sbjct: 63  IGGLLSLKFSIRKVLLVTSPAAVNECFTKNDIVFANHPSTLSTKHFSYNYTTISIAPYGD 122

Query: 394 HWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEIV 573
           HWRNLR LAALEIFS +RI+ F  +R +ELM+LL+E  R+ K  GG T VDL SKF E+ 
Sbjct: 123 HWRNLRCLAALEIFSPSRITTFTNIRRRELMLLLNEFLRDLKNGGGKTMVDLKSKFIELA 182

Query: 574 FNVLSMTIAGKRYYGENVVDARKVR 648
           F+VLSMTIAGKRYYGENV DA + R
Sbjct: 183 FDVLSMTIAGKRYYGENVEDAEEAR 207


>ref|XP_024166813.1| cytochrome P450 81E8-like [Rosa chinensis]
 gb|PRQ27780.1| putative oxidoreductase [Rosa chinensis]
          Length = 511

 Score =  207 bits (528), Expect = 6e-61
 Identities = 101/205 (49%), Positives = 138/205 (67%), Gaps = 2/205 (0%)
 Frame = +1

Query: 40  TLWYIILSAIFI--LILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQALSEK 213
           +LW+  L+ I    LI K F++N Q              IGHLHL+K+P HR LQALSE 
Sbjct: 4   SLWFSALAFIIFITLIYKLFISNRQKYHNLPPSPLSLPIIGHLHLLKQPAHRTLQALSEN 63

Query: 214 YGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAPYGD 393
           YG +L L+ G RKVLL+ +PSA EECFT+NDIVF  RP  L  K F YD+TT+S APYGD
Sbjct: 64  YGKVLLLRWGSRKVLLLTTPSAAEECFTKNDIVFTNRPRLLGGKDFHYDYTTVSFAPYGD 123

Query: 394 HWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFTEIV 573
            WRN+RR+  LE+FS++R+  F+ VR +E+ ++L ++ R S    G  KVDL +KF E+ 
Sbjct: 124 LWRNIRRVMTLELFSSSRLPAFQTVRQEEVRVVLDQIMRRSSNDNGMRKVDLKTKFLELS 183

Query: 574 FNVLSMTIAGKRYYGENVVDARKVR 648
           FN ++MT+ G+R+YGE+VVD  + +
Sbjct: 184 FNAMTMTMVGRRFYGEDVVDQAEAK 208


>gb|PON48894.1| Cytochrome P450, E-class, group I [Parasponia andersonii]
          Length = 337

 Score =  201 bits (512), Expect = 2e-60
 Identities = 104/211 (49%), Positives = 143/211 (67%), Gaps = 5/211 (2%)
 Frame = +1

Query: 31  MEITLWYII-LSAIFILILKYFLANTQXXXXXXXXXXXXXX-IGHLHLVKEPLHRNLQAL 204
           ME T +Y+  LS + I+I+ +   +T+               IGHLHL+KEP+HR LQ+L
Sbjct: 1   MENTYFYLYSLSTLVIIIILFIKFSTRNKRSRLPPSPPSLPFIGHLHLLKEPIHRTLQSL 60

Query: 205 SEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAP 384
           S KYG +L L  G R +LLV SPSA EECFT+NDIVFA RP TLA KHF YD +T++ A 
Sbjct: 61  SNKYGKVLLLNCGSRTILLVSSPSAAEECFTKNDIVFANRPHTLAGKHFHYDHSTVASAS 120

Query: 385 YGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFT 564
           YGDHWRNLRR+  LE+FS++R++ +  VR  E+ + +SE+        G+ K+DL SKFT
Sbjct: 121 YGDHWRNLRRVMTLELFSSSRLAAYSTVRQDEIRLSVSEI--KQSCEQGTRKIDLKSKFT 178

Query: 565 EIVFNVLSMTIAGKRYYGENVVD---ARKVR 648
           E+ FNV++M + GKR+YG+NV D   A+++R
Sbjct: 179 ELSFNVMTMILVGKRFYGDNVEDFGEAQRIR 209


>ref|XP_007227313.2| cytochrome P450 81D11 [Prunus persica]
 gb|ONI33501.1| hypothetical protein PRUPE_1G428700 [Prunus persica]
          Length = 507

 Score =  205 bits (521), Expect = 6e-60
 Identities = 104/208 (50%), Positives = 141/208 (67%), Gaps = 2/208 (0%)
 Frame = +1

Query: 31  MEITLWYIILSAIFILILKYFLANT--QXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQAL 204
           ME   WY +   IF L+LK        +              IGHLHLVK+P+HR LQAL
Sbjct: 1   MEEPFWYGLALIIFTLLLKLSTTKLGQKHKKNLPPSPPSLPMIGHLHLVKQPIHRTLQAL 60

Query: 205 SEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAP 384
           +EK+G +L L+ G R+VLLV SPS  EE FT++DI+FA+RP  L  KHF+YDFTT+++AP
Sbjct: 61  AEKFGKVLLLRWGSRRVLLVSSPSVAEELFTKHDIIFASRPRLLVGKHFAYDFTTVTLAP 120

Query: 385 YGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFT 564
           YGD WRNLRR+  LE+FS+AR++ F  +R  E+ +LL++  ++   S   TKV+L SKFT
Sbjct: 121 YGDLWRNLRRVMTLEVFSSARLAQFSSIRQGEVRLLLNQTVKSCAKS--MTKVELKSKFT 178

Query: 565 EIVFNVLSMTIAGKRYYGENVVDARKVR 648
           E+ FNV++M   GKRYYGENV+DA + +
Sbjct: 179 ELSFNVMTMMAVGKRYYGENVLDAEEAK 206


>gb|PON31981.1| Cytochrome P450, E-class, group I, partial [Trema orientalis]
          Length = 304

 Score =  198 bits (503), Expect = 2e-59
 Identities = 101/205 (49%), Positives = 137/205 (66%), Gaps = 4/205 (1%)
 Frame = +1

Query: 31  MEITLWYII----LSAIFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQ 198
           ME T +Y+     L  I IL +K+   N +              IGHLHL+KEP+HR LQ
Sbjct: 1   MENTYFYLYSWSTLVIIIILFIKFSKKNKRSRLPPSPPSLPL--IGHLHLLKEPIHRTLQ 58

Query: 199 ALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISV 378
           +LS K+G +L L  G R +LLV SPSA EECFT+NDIVFA RP TLA KHF YD +T++ 
Sbjct: 59  SLSNKHGKVLLLNCGSRTILLVSSPSAAEECFTKNDIVFANRPHTLAGKHFHYDHSTVAS 118

Query: 379 APYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSK 558
           A YGDHWRNLRR+  LE+FS++R++ +  VR  E+ +L++++        G+ K+DL SK
Sbjct: 119 ASYGDHWRNLRRVMTLELFSSSRLAAYSTVRQDEIRLLVNQI--KQSCEQGTRKIDLKSK 176

Query: 559 FTEIVFNVLSMTIAGKRYYGENVVD 633
           FTE+ FNV++M + GKR+YG+NV D
Sbjct: 177 FTELSFNVMTMILVGKRFYGDNVED 201


>ref|XP_021820295.1| isoflavone 3'-hydroxylase-like [Prunus avium]
          Length = 511

 Score =  204 bits (518), Expect = 2e-59
 Identities = 104/208 (50%), Positives = 141/208 (67%), Gaps = 2/208 (0%)
 Frame = +1

Query: 31  MEITLWY-IILSAIFILILKYFLAN-TQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQAL 204
           ME   WY + L  IF L+LK  +A  TQ              IGHLHL+K+P+HR LQAL
Sbjct: 1   MEEPFWYSLALIIIFTLLLKLSIAKITQKHNNFPPSPPSLPIIGHLHLLKQPIHRTLQAL 60

Query: 205 SEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAP 384
           S K+G +L L+ G R+VLLV SPS  EE FT++DI+FA RP  L  KH +YDFTT+S+AP
Sbjct: 61  SGKFGKLLLLRWGSRRVLLVSSPSLAEELFTKHDIIFANRPRLLIGKHLAYDFTTVSLAP 120

Query: 385 YGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFT 564
           YGD WRNLRR   LE+FS +R++ F  +R  E+ +LL+E+ + S  +   TK++L SKFT
Sbjct: 121 YGDLWRNLRRTMTLELFSASRLAQFSSIRQGEVRLLLNEIMKKS-CTERKTKIELKSKFT 179

Query: 565 EIVFNVLSMTIAGKRYYGENVVDARKVR 648
           E+ FNV++M + GKR+YGENV+D  + +
Sbjct: 180 ELSFNVMTMMVVGKRFYGENVLDVEEAK 207


>gb|POF08330.1| cytochrome p450 81e8 [Quercus suber]
          Length = 242

 Score =  195 bits (496), Expect = 3e-59
 Identities = 101/208 (48%), Positives = 135/208 (64%), Gaps = 2/208 (0%)
 Frame = +1

Query: 31  MEITLWYIILSAIFILIL--KYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQAL 204
           ME  L Y  LS + +L +  K+ L                  +GHLHL+K+P HR  +  
Sbjct: 1   MEDMLLYSSLSLLLLLAVAFKFLLQTRTKHKHLPPSPPSLPILGHLHLLKKPFHRTFRHF 60

Query: 205 SEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVAP 384
           S+KYGNI  L+ G + V++V SPSAV+ECFT+NDI+ A RP  LATKH +Y+ TT++ A 
Sbjct: 61  SQKYGNIFSLKFGSQLVVVVSSPSAVDECFTKNDIILANRPRFLATKHIAYNHTTVAAAS 120

Query: 385 YGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKFT 564
           YGDHWRNLRR++ALEIFST R++MF  +RT E+  LL +L RNS    G  KV+L S FT
Sbjct: 121 YGDHWRNLRRISALEIFSTNRLNMFLGIRTDEVKHLLHKLSRNS--CQGFAKVELKSMFT 178

Query: 565 EIVFNVLSMTIAGKRYYGENVVDARKVR 648
           E+ FN++   + GKRYYGE+V D  + R
Sbjct: 179 ELTFNIIMRMVVGKRYYGEDVKDEEEAR 206


>ref|XP_017605887.1| PREDICTED: isoflavone 2'-hydroxylase-like [Gossypium arboreum]
          Length = 517

 Score =  202 bits (515), Expect = 6e-59
 Identities = 104/206 (50%), Positives = 140/206 (67%), Gaps = 3/206 (1%)
 Frame = +1

Query: 31  MEITLWYIILSA---IFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQA 201
           ME+TL+ IIL     +F L+ K  +                  IGHLHL+KEP HR L  
Sbjct: 1   MEVTLFNIILCISIFLFALVFKSIVQKCPTPRNLPPSPPALPIIGHLHLLKEPFHRTLHQ 60

Query: 202 LSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISVA 381
           LS+KYG IL LQ G RKVL+V S SA EECFT+NDI+FA RP  LA KH +Y+++TI +A
Sbjct: 61  LSQKYGPILLLQFGTRKVLIVSSASAAEECFTKNDIIFANRPQMLAGKHLNYNYSTIGLA 120

Query: 382 PYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSKF 561
           PYGD+WRNL+RL  +E+FST+R++MF  +R +E+ +LL ELF  S  +    KV+L+SK 
Sbjct: 121 PYGDYWRNLKRLTTVELFSTSRLAMFAGIRQEEMQLLLKELFLAS--TRKPAKVELSSKL 178

Query: 562 TEIVFNVLSMTIAGKRYYGENVVDAR 639
            ++VFN++   IAGKRYYG++VVD +
Sbjct: 179 MDLVFNIILRMIAGKRYYGKDVVDKK 204


>ref|XP_004290938.1| PREDICTED: isoflavone 3'-hydroxylase-like [Fragaria vesca subsp.
           vesca]
          Length = 543

 Score =  203 bits (516), Expect = 7e-59
 Identities = 99/211 (46%), Positives = 143/211 (67%), Gaps = 2/211 (0%)
 Frame = +1

Query: 22  AKKMEITLWYIILSAIFIL--ILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNL 195
           + KM  +LW+  L+ I  +  I K F++N Q              IGHLHL+K+P+HR L
Sbjct: 29  SNKMVDSLWFSSLALIIFITVIHKLFISNEQKYHNLPPSPPSLPIIGHLHLLKQPVHRTL 88

Query: 196 QALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTIS 375
           Q+LSE YG +L L+ G RKVLL+ SPSA EECFT+NDI+F  RP  LA K   YD+TT++
Sbjct: 89  QSLSETYGKVLLLRWGSRKVLLLTSPSAAEECFTKNDIIFTNRPRMLAGKVLHYDYTTVT 148

Query: 376 VAPYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNS 555
            AP+GD WRNLRR+  LE+FS++R++ F+ VR +E+ ++L ++ R S  + G  KVDL +
Sbjct: 149 FAPHGDLWRNLRRVMTLELFSSSRLAAFQSVREEEVRVVLDQIMRRSVNNNGMRKVDLKT 208

Query: 556 KFTEIVFNVLSMTIAGKRYYGENVVDARKVR 648
           KF E+ FN ++MT+ G+R+YGE+V D  + +
Sbjct: 209 KFLELSFNAMTMTMVGRRFYGEDVEDQAEAK 239


>ref|XP_002510139.1| PREDICTED: isoflavone 2'-hydroxylase [Ricinus communis]
 gb|EEF52326.1| cytochrome P450, putative [Ricinus communis]
          Length = 514

 Score =  202 bits (514), Expect = 7e-59
 Identities = 100/207 (48%), Positives = 143/207 (69%), Gaps = 6/207 (2%)
 Frame = +1

Query: 31  MEITLWY----IILSAIFILI--LKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRN 192
           ME++LW+    +++  +F+L+  L +F    +              +GHLHL+K+P+HR 
Sbjct: 1   MEVSLWFKLAVLVVVLLFVLVPKLTFFHKLDRSPKNLPPSPPARPIVGHLHLLKQPVHRT 60

Query: 193 LQALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTI 372
           L  LS KYG+IL L+ G RKVL++ SPSA+EECFTRND++FA RP +LA KH +Y+ TT+
Sbjct: 61  LHELSSKYGDILLLRYGTRKVLVISSPSAIEECFTRNDVIFANRPQSLAGKHLNYNSTTM 120

Query: 373 SVAPYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLN 552
             + YGDHWRNLRRL  +E+FSTA  +MF  +R +E+ +LL +LF++S+  G S KV L 
Sbjct: 121 GFSSYGDHWRNLRRLTTVELFSTAHTAMFSGIRAEEVRLLLKQLFQDSR--GESAKVALT 178

Query: 553 SKFTEIVFNVLSMTIAGKRYYGENVVD 633
           SKF ++ FN +   IAGKRYYG++VVD
Sbjct: 179 SKFLDLTFNNMMRIIAGKRYYGKDVVD 205


>ref|XP_012068273.1| isoflavone 2'-hydroxylase [Jatropha curcas]
 gb|KDP41667.1| hypothetical protein JCGZ_16074 [Jatropha curcas]
          Length = 520

 Score =  202 bits (514), Expect = 8e-59
 Identities = 102/209 (48%), Positives = 138/209 (66%), Gaps = 8/209 (3%)
 Frame = +1

Query: 31  MEITLW--------YIILSAIFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLH 186
           ME+T W         ++   +F+L  K+     +              IGHLH++K+P+H
Sbjct: 1   MEVTYWSNTAALVLVLVPVLLFVLAAKFSFQKLKRSKKFPPSPPARPVIGHLHMLKQPIH 60

Query: 187 RNLQALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFT 366
           R L  LSEKYG+IL L+ G RKVLL+ SPSA EECFTRNDI+FA RP +LA K+ +YD T
Sbjct: 61  RTLHQLSEKYGDILFLRYGTRKVLLISSPSAAEECFTRNDIIFANRPRSLAGKYLNYDST 120

Query: 367 TISVAPYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVD 546
           T+  + YGDHWRNLRRL  +E+FST+R +MF  +RT+E+ +LL +LF++S+      KV 
Sbjct: 121 TMGFSSYGDHWRNLRRLTTIELFSTSRATMFSGIRTEEVRLLLKQLFQDSRKE--PAKVA 178

Query: 547 LNSKFTEIVFNVLSMTIAGKRYYGENVVD 633
           L SKF E+ FN +   IAGKRYYG+NV+D
Sbjct: 179 LTSKFLELTFNNIMRMIAGKRYYGKNVMD 207


>ref|XP_022772222.1| isoflavone 2'-hydroxylase-like [Durio zibethinus]
          Length = 519

 Score =  201 bits (510), Expect = 3e-58
 Identities = 107/206 (51%), Positives = 138/206 (66%), Gaps = 5/206 (2%)
 Frame = +1

Query: 31  MEI-TLWYIILSA----IFILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNL 195
           MEI +L+YII  +    +F L+LK  +                  IGHLHL+KEP+HR L
Sbjct: 1   MEIASLFYIITLSFPIFLFALVLKSIIQIRNSPRNFPPSPPALPIIGHLHLLKEPIHRTL 60

Query: 196 QALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTIS 375
             LSEKYG IL LQ G RKVL+V S SA EEC T+NDI+FA RP  LA KH +Y+ TT+ 
Sbjct: 61  HELSEKYGPILYLQFGTRKVLIVSSASAAEECLTKNDIIFANRPQMLAGKHLNYNNTTMG 120

Query: 376 VAPYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNS 555
            APYGD+WRNLRRL  LE+FST+R+ MF  +R +E+ +LL +LF  S+    S KV++ S
Sbjct: 121 FAPYGDYWRNLRRLTTLELFSTSRLDMFASIRQEEVQLLLKDLFLASRRK--SAKVEVTS 178

Query: 556 KFTEIVFNVLSMTIAGKRYYGENVVD 633
           K  E+VFN++  TI GKRYYG++VVD
Sbjct: 179 KLIELVFNIILRTIGGKRYYGKDVVD 204


>gb|POE89360.1| isoflavone 2'-hydroxylase [Quercus suber]
          Length = 383

 Score =  196 bits (499), Expect = 6e-58
 Identities = 94/161 (58%), Positives = 122/161 (75%)
 Frame = +1

Query: 151 IGHLHLVKEPLHRNLQALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPF 330
           IGHLHL+KEPLHR L  LSEKYG++L L+ G RKVL++ SPSAVEECFT+ DI+FA RP 
Sbjct: 43  IGHLHLLKEPLHRTLHELSEKYGHVLFLRCGIRKVLVISSPSAVEECFTKKDIIFANRPR 102

Query: 331 TLATKHFSYDFTTISVAPYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFR 510
            LA KH +Y++ TI  + YGDHWRNLRRL  LE+FST+R++MF  VR  E+ +L+ +LF+
Sbjct: 103 MLAGKHLNYNYKTIGFSSYGDHWRNLRRLTTLELFSTSRLAMFTSVRQDEVRLLVKQLFQ 162

Query: 511 NSKISGGSTKVDLNSKFTEIVFNVLSMTIAGKRYYGENVVD 633
           +S      TKV+L  K  ++ FN++  TIAGKRYYG+ VVD
Sbjct: 163 DSS-REKPTKVELRPKLLDLSFNIMMRTIAGKRYYGKEVVD 202


>ref|XP_016749332.1| PREDICTED: isoflavone 2'-hydroxylase-like [Gossypium hirsutum]
 ref|XP_016749333.1| PREDICTED: isoflavone 2'-hydroxylase-like [Gossypium hirsutum]
          Length = 518

 Score =  199 bits (507), Expect = 9e-58
 Identities = 103/207 (49%), Positives = 138/207 (66%), Gaps = 4/207 (1%)
 Frame = +1

Query: 31  MEITLWYIILSAI----FILILKYFLANTQXXXXXXXXXXXXXXIGHLHLVKEPLHRNLQ 198
           ME+T  + I+  I    F L+ K  +                  IGHLHL+KEP HR L 
Sbjct: 1   MEVTTLFNIILCISIFLFALVFKSIVQKCPTPRNLPPSPPALPIIGHLHLLKEPFHRTLH 60

Query: 199 ALSEKYGNILQLQLGFRKVLLVDSPSAVEECFTRNDIVFATRPFTLATKHFSYDFTTISV 378
            LS+KYG IL LQ G RKVL+V S SA EECFT+NDI+FA RP  LA KH +Y+++TI +
Sbjct: 61  QLSQKYGPILLLQFGTRKVLIVSSASAAEECFTKNDIIFANRPQMLAGKHLNYNYSTIGL 120

Query: 379 APYGDHWRNLRRLAALEIFSTARISMFEKVRTKELMILLSELFRNSKISGGSTKVDLNSK 558
           APYGD+WRNL+RL  +E+FST+R++MF  +R +E+ +LL ELF  S  +    KV+L+SK
Sbjct: 121 APYGDYWRNLKRLTTVELFSTSRLAMFAGIRQEEVQLLLKELFLAS--TRKPAKVELSSK 178

Query: 559 FTEIVFNVLSMTIAGKRYYGENVVDAR 639
             ++VFN++   IAGKRYYGE+VVD +
Sbjct: 179 LMDLVFNIILRMIAGKRYYGEDVVDKK 205